RLG00000028252

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000598
Physical Location & Seq
Forward (+)
20550788 .. 20552506
1719 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028252

Sequence Viewer

Length: 1719 bp
ATGAATGGTTTCCTTCATCTGGGACATGCATTCACCATCTCCAAGCTTGAGTTTGCTGCCTCTTATCATAGGCTAAGAGGTGTCAATGTGCTCTTACCATTTGCTTTCCATTGCACCGGAATGCCCATCAAAGCTGCTGCCGATAAACTTGCAAGAGAGCTTCAACAGTTTGGCTACCCTCCAGTATTTCCCATTGATGTGAAGCAAGATAAAATTGAAGCCGAAGCAAAAGCCAAAGCTGGAGCAGAGAAAAAACATAAGGGAAAGAAGTGCAAGGCTGCAGCAAAGTCCTCCGGACAAGTCTTCCAATGGGAAATTATGCGTGATGGTTTTGGATTGTCTGACAGTGAGATATCCCAGTTCCAGGACCCGGAACATTGGTTGAAGTTTTTCCCACCACGTGGGGTGGAAGACCTGAAAGCTTTTGGGTTGGGATGTGATTGGAGACGTTCCTTTATTACTACTGACATGAACCCTTTCTTTGACAACTTTGTGAAATGGCAGGTTAGGAAACTCAAATCCAAGGGTAAGATTGTGAAAGATGTCCGGTACACTATATTCTCCCCATTCGATGGGCAGCCGTGTGCAGATCATGATAGGGCAAGAGGAGAAGGAGTTCAGCCACAAGAATACATTGTCATCAAAATGGAGGTAGTAGCACCTTTACCCTCAAAATTTGGATCAGTGTTGAAAGGGAGGAAGGTATTCCTAGCAGCAGCAACGTTGAGACCCGAGACCATGTATGGACAAACAAATGCATGGGTATTACCCGATGCTAAGTATGGAGCCTTTGAGATCAACGATACGGATGTCTTTATTCTTACACATAGAGCAGCACTTAATCTTGCCTATCAGAAGTACTCTAGGGTCCCAGAGGAACCTACTTGCTTGGTCGAACTGACTGGTTATGATTTGATTGGCCTTCCATTGAAGTCACCACTTGCATTCAATCAGATCATATATGCCCTTCCTATGTTGATCATCCCAACTGACAAAGGCACTGGGATAGTCACCAGCGTTCCTAGTGATGCTCCTGATGATTATATGGCTCTGCATAATTTAAAGTCAAAATCTGCTTTGAGGGAAAAATATGGTGTGAAGGAAGAATGGGTGATGCCGTTTGAAGTTATCCCAATCATCAAAGAAGGAACACTGACCGTGGGAGAATTCGTAGGAAGGGAAGTGCAGGAGGCGAAACCCTTAATAAAAAGGAAGCTGATAGAGGTGGGAGAAGGAATTATATATTATGAACCTGAGGAGCAAGTCGTGTCCCGGTCGGGTGATGAATGTGTTGTGGCACTTACAGATCAGTGGTACATCACTTATGGGGAAACGGAATGGAAAAAGATGGCTGAAGAGTGCTTGTCAGGCATGAACTTATATTCCAATGAGACTCGGCATGGATTTGAGCACACTATAGGGTGGTTGAATCAGTGGGCTTGTTCACGATCTTTTGGACTTGGGACTCGTATCCCCTGGGATGAAGAATTCTTAGTTGAGTCACTGTCTGATTCAACTATTTACATGGCTTATTACACCATTGCTCATTTCTTACACAATGGAGATATGTATGGTGCCAGCAGGTCAGAAGTTAAACCTGAGCAAATGACTGACGACGTCTGGGATTTTATTTTCTGTGATGGCCCATATCCCAAGTCATCTGACATCTCATCATCAATTCTCATCATCAATTCTAAAGAAAATGAAGAAAGAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

573

Amino Acids

64.76

Weight (kDa)

5.82

Isoelectric Point (pI)

41.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1 PF00133 137 - 492 5.8e-07 tRNA synthetases class I (I, L, M and V)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000598)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09620
fragaria_vesca FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_3g34581 FvH4_4g33720 FvH4_4g33720
malus_domestica MD08G1244600.v1.1 MD15G1439400.v1.1
prunus_persica Prupe.1G582000_v2.0.a1 Prupe.2G015100_v2.0.a1 Prupe.2G123100_v2.0.a1
pyrus_communis pycom08g21210 pycom08g21220 pycom15g38730
rosa_chinensis RchiOBHm_Chr1g0336121 RchiOBHm_Chr1g0353811 RchiOBHm_Chr1g0373331 RchiOBHm_Chr4g0443191 RchiOBHm_Chr4g0443201 RchiOBHm_Chr4g0443211
rosa_laevigata RLG00000003122 RLG00000005948 RLG00000026826 RLG00000028251 RLG00000028252 RLG00000028253 RLG00000029377
rosa_multiflora Rmu_co8002498.1_g000001 Rmu_co8262279.1_g000001 Rmu_co8314139.1_g000001 Rmu_co8393711.1_g000001 Rmu_co8419167.1_g000001 Rmu_sc0007968.1_g000004 Rmu_sc0018919.1_g000004 Rmu_sc0027447.1_g000001
rosa_roxburghii Rroxscaffold_4G00284260 Rroxscaffold_4G00301490 Rroxscaffold_4G00315860 Rroxscaffold_5G00383790 Rroxscaffold_5G00383800
rosa_rugosa Rorug01G0123100 Rorug01G0238700 Rorug01G0377100 Rorug01G0377100 Rorug01G0377200 Rorug01G0377300 Rorug04G0344700 Rorug05G0347900.1
rosa_samantha Rh1AG143800 Rh1AG250300 Rh1AG386300 Rh1BG113400 Rh1BG220800 Rh1BG220900 Rh1BG350400 Rh1CG135500 Rh1CG233100 Rh1CG363500 Rh1DG150200 Rh1DG247700 Rh1DG247800 Rh1DG381300 Rh4AG399400 Rh4AG399500 Rh4BG411000 Rh4BG411100 Rh4BG411200 Rh4CG426500 Rh4CG426600 Rh4DG406100 Rh4DG406200 Rh4DG406300
rosa_wichuraiana Rw0G015190 Rw1G021850 Rw1G034870 Rw4G034440 Rw4G034450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1620
Acc36I ACCTGC 2 cut(s) 493, 1572
AccB1I GGYRCC 1 cut(s) 1574
AccB7I CCANNNNNTGG 2 cut(s) 401, 572
AccIII TCCGGA 1 cut(s) 293
AclI AACGTT 1 cut(s) 722
AclWI GGATC 1 cut(s) 688
AcsI RAATTY 3 cut(s) 674, 1166, 1487
AcuI CTGAAG 1 cut(s) 1374
AcvI CACGTG 1 cut(s) 401
AcyI GRCGYC 1 cut(s) 1617
AdeI CACNNNGTG 1 cut(s) 401
AfaI GTAC 3 cut(s) 551, 860, 1316
AfiI CCNNNNNNNGG 5 cut(s) 19, 364, 370, 401, 572
AgsI TTSAA 9 cut(s) 164, 218, 385, 691, 931, 949, 1124, 1429, 1515
AjnI CCWGG 2 cut(s) 363, 1475
AloI GAACNNNNNNTCC 4 cut(s) 600, 632, 1367, 1399
AluBI AGCT 6 cut(s) 46, 134, 160, 239, 422, 1216
AluI AGCT 6 cut(s) 46, 134, 160, 239, 422, 1216
Alw21I GWGCWC 2 cut(s) 93, 1413
Alw26I GTCTC 4 cut(s) 439, 721, 728, 1385
AlwI GGATC 1 cut(s) 688
Ama87I CYCGRG 1 cut(s) 731
Aor13HI TCCGGA 1 cut(s) 293
AoxI GGCC 2 cut(s) 919, 1642
ApeKI GCWGC 9 cut(s) 56, 134, 137, 278, 281, 577, 713, 716, 833
ApoI RAATTY 3 cut(s) 674, 1166, 1487
Asp700I GAANNNNTTC 5 cut(s) 8, 389, 476, 615, 704
AspS9I GGNCC 3 cut(s) 367, 868, 1643
AsuC2I CCSGG 2 cut(s) 371, 1273
AsuHPI GGTGA 5 cut(s) 25, 927, 1003, 1123, 1292
AvaI CYCGRG 1 cut(s) 731
AvaII GGWCC 2 cut(s) 367, 868
AxyI CCTNAGG 1 cut(s) 1254
BaeI ACNNNNGTAYC 2 cut(s) 795, 828
BanI GGYRCC 1 cut(s) 1574
BbrPI CACGTG 1 cut(s) 401
BbsI GAAGAC 2 cut(s) 295, 417
Bbv12I GWGCWC 2 cut(s) 93, 1413
BbvI GCAGC 9 cut(s) 43, 121, 124, 265, 293, 589, 725, 728, 845
BccI CCATC 6 cut(s) 44, 134, 320, 566, 1342, 1634
BceAI ACGGC 2 cut(s) 565, 1102
BcgI CGANNNNNNTGC 2 cut(s) 131, 165
BciT130I CCWGG 2 cut(s) 365, 1477
BciVI GTATCC 1 cut(s) 1481
BclI TGATCA 1 cut(s) 978
BcnI CCSGG 2 cut(s) 371, 1273
BcoDI GTCTC 4 cut(s) 439, 721, 728, 1385
BfaI CTAG 3 cut(s) 710, 864, 1023
BfmI CTRYAG 2 cut(s) 279, 1416
BfuAI ACCTGC 2 cut(s) 493, 1572
BfuI GTATCC 1 cut(s) 1481
BisI GCNGC 9 cut(s) 57, 135, 138, 279, 282, 578, 714, 717, 834
BlsI GCNGC 9 cut(s) 58, 136, 139, 280, 283, 579, 715, 718, 835
BmcAI AGTACT 1 cut(s) 860
Bme1390I CCNGG 4 cut(s) 365, 371, 1273, 1477
Bme18I GGWCC 2 cut(s) 367, 868
BmeT110I CYCGRG 1 cut(s) 731
BmgT120I GGNCC 3 cut(s) 367, 868, 1643
BmiI GGNNCC 6 cut(s) 369, 787, 869, 870, 879, 1576
BmrFI CCNGG 4 cut(s) 365, 371, 1273, 1477
BmrI ACTGGG 2 cut(s) 352, 1011
BmsI GCATC 3 cut(s) 763, 1018, 1104
BmuI ACTGGG 2 cut(s) 352, 1011
BpiI GAAGAC 2 cut(s) 295, 417
BpmI CTGGAG 2 cut(s) 165, 261
Bpu10I CCTNAGC 1 cut(s) 1599
BpuEI CTTGAG 1 cut(s) 68
BpuMI CCSGG 2 cut(s) 371, 1273
BsaAI YACGTR 1 cut(s) 401
BsaHI GRCGYC 1 cut(s) 1617
BsaI GGTCTC 2 cut(s) 721, 728
BsaJI CCNNGG 4 cut(s) 522, 1158, 1475, 1476
BsaWI WCCGGW 3 cut(s) 116, 293, 546
BsaXI ACNNNNNCTCC 4 cut(s) 600, 630, 1155, 1185
Bsc4I CCNNNNNNNGG 5 cut(s) 19, 364, 370, 401, 572
Bse1I ACTGG 4 cut(s) 182, 358, 907, 1006
Bse21I CCTNAGG 1 cut(s) 1254
Bse3DI GCAATG 2 cut(s) 109, 1539
BseAI TCCGGA 1 cut(s) 293
BseBI CCWGG 2 cut(s) 365, 1477
BseDI CCNNGG 4 cut(s) 522, 1158, 1475, 1476
BseGI GGATG 4 cut(s) 440, 814, 981, 1486
BseLI CCNNNNNNNGG 5 cut(s) 19, 364, 370, 401, 572
BseMI GCAATG 2 cut(s) 109, 1539
BseMII CTCAG 2 cut(s) 1245, 1590
BseNI ACTGG 4 cut(s) 182, 358, 907, 1006
BseRI GAGGAG 2 cut(s) 621, 1271
BseXI GCAGC 9 cut(s) 43, 121, 124, 265, 293, 589, 725, 728, 845
BsgI GTGCAG 2 cut(s) 606, 1205
Bsh1285I CGRYCG 1 cut(s) 1277
BshFI GGCC 2 cut(s) 921, 1644
BshNI GGYRCC 1 cut(s) 1574
BsiEI CGRYCG 1 cut(s) 1277
BsiHKAI GWGCWC 2 cut(s) 93, 1413
BsiHKCI CYCGRG 1 cut(s) 731
BsiSI CCGG 5 cut(s) 117, 294, 371, 547, 1273
BslFI GGGAC 4 cut(s) 36, 854, 1255, 1477
BslI CCNNNNNNNGG 5 cut(s) 19, 364, 370, 401, 572
BsmAI GTCTC 4 cut(s) 439, 721, 728, 1385
BsmBI CGTCTC 1 cut(s) 439
BsmFI GGGAC 4 cut(s) 36, 854, 1255, 1477
BsmI GAATGC 3 cut(s) 29, 126, 944
BsnI GGCC 2 cut(s) 921, 1644
Bso31I GGTCTC 2 cut(s) 721, 728
BsoBI CYCGRG 1 cut(s) 731
Bsp1286I GDGCHC 2 cut(s) 93, 1413
Bsp13I TCCGGA 1 cut(s) 293
Bsp143I GATC 7 cut(s) 589, 680, 795, 954, 978, 1306, 1448
BspANI GGCC 2 cut(s) 921, 1644
BspCNI CTCAG 2 cut(s) 1246, 1591
BspEI TCCGGA 1 cut(s) 293
BspHI TCATGA 1 cut(s) 592
BspLI GGNNCC 6 cut(s) 369, 787, 869, 870, 879, 1576
BspMAI CTGCAG 1 cut(s) 283
BspMI ACCTGC 2 cut(s) 493, 1572
BspPI GGATC 1 cut(s) 688
BspT107I GGYRCC 1 cut(s) 1574
BspTNI GGTCTC 2 cut(s) 721, 728
BsrDI GCAATG 2 cut(s) 109, 1539
BsrI ACTGG 4 cut(s) 182, 358, 907, 1006
BssECI CCNNGG 4 cut(s) 522, 1158, 1475, 1476
BssMI GATC 7 cut(s) 589, 680, 795, 954, 978, 1306, 1448
BssNI GRCGYC 1 cut(s) 1617
BssT1I CCWWGG 1 cut(s) 522
Bst2UI CCWGG 2 cut(s) 365, 1477
Bst4CI ACNGT 4 cut(s) 168, 347, 1159, 1506
Bst6I CTCTTC 1 cut(s) 1350
BstACI GRCGYC 1 cut(s) 1617
BstBAI YACGTR 1 cut(s) 401
BstC8I GCNNGC 1 cut(s) 1579
BstDEI CTNAG 5 cut(s) 74, 777, 1254, 1492, 1599
BstDSI CCRYGG 1 cut(s) 1158
BstF5I GGATG 4 cut(s) 440, 814, 981, 1486
BstKTI GATC 7 cut(s) 592, 683, 798, 957, 981, 1309, 1451
BstMAI GTCTC 4 cut(s) 439, 721, 728, 1385
BstMBI GATC 7 cut(s) 589, 680, 795, 954, 978, 1306, 1448
BstMCI CGRYCG 1 cut(s) 1277
BstMWI GCNNNNNNNGC 1 cut(s) 1368
BstNI CCWGG 2 cut(s) 365, 1477
BstNSI RCATGY 1 cut(s) 29
BstSCI CCNGG 4 cut(s) 363, 369, 1271, 1475
BstSFI CTRYAG 2 cut(s) 279, 1416
BstV1I GCAGC 9 cut(s) 43, 121, 124, 265, 293, 589, 725, 728, 845
BstV2I GAAGAC 2 cut(s) 295, 417
Bsu36I CCTNAGG 1 cut(s) 1254
BsuI GTATCC 1 cut(s) 1481
BsuRI GGCC 2 cut(s) 921, 1644
BtgI CCRYGG 1 cut(s) 1158
BtsCI GGATG 4 cut(s) 440, 814, 981, 1486
BtsIMutI CAGTG 7 cut(s) 352, 690, 999, 1151, 1316, 1439, 1502
BveI ACCTGC 2 cut(s) 493, 1572
Cac8I GCNNGC 1 cut(s) 1579
CciI TCATGA 1 cut(s) 592
Cfr13I GGNCC 3 cut(s) 367, 868, 1643
Csp6I GTAC 3 cut(s) 550, 859, 1315
CviAII CATG 8 cut(s) 26, 469, 593, 739, 759, 1372, 1400, 1525
CviQI GTAC 3 cut(s) 550, 859, 1315
DdeI CTNAG 5 cut(s) 74, 777, 1254, 1492, 1599
DpnI GATC 7 cut(s) 591, 682, 797, 956, 980, 1308, 1450
DpnII GATC 7 cut(s) 589, 680, 795, 954, 978, 1306, 1448
DraI TTTAAA 1 cut(s) 1062
DraIII CACNNNGTG 1 cut(s) 401
Eam1104I CTCTTC 1 cut(s) 1350
EarI CTCTTC 1 cut(s) 1350
Eco130I CCWWGG 1 cut(s) 522
Eco31I GGTCTC 2 cut(s) 721, 728
Eco32I GATATC 1 cut(s) 354
Eco47I GGWCC 2 cut(s) 367, 868
Eco57I CTGAAG 1 cut(s) 1374
Eco72I CACGTG 1 cut(s) 401
Eco81I CCTNAGG 1 cut(s) 1254
Eco88I CYCGRG 1 cut(s) 731
EcoO109I RGGNCCY 2 cut(s) 367, 868
EcoRI GAATTC 2 cut(s) 1166, 1487
EcoRII CCWGG 2 cut(s) 363, 1475
EcoRV GATATC 1 cut(s) 354
EcoT14I CCWWGG 1 cut(s) 522
EcoT22I ATGCAT 2 cut(s) 31, 760
ErhI CCWWGG 1 cut(s) 522
Esp3I CGTCTC 1 cut(s) 439
FaeI CATG 8 cut(s) 29, 472, 596, 742, 762, 1375, 1403, 1528
FaqI GGGAC 4 cut(s) 36, 854, 1255, 1477
FatI CATG 8 cut(s) 25, 468, 592, 738, 758, 1371, 1399, 1524
FbaI TGATCA 1 cut(s) 978
Fnu4HI GCNGC 9 cut(s) 57, 135, 138, 279, 282, 578, 714, 717, 834
FokI GGATG 4 cut(s) 447, 821, 968, 1493
Fsp4HI GCNGC 9 cut(s) 57, 135, 138, 279, 282, 578, 714, 717, 834
FspBI CTAG 3 cut(s) 710, 864, 1023
GluI GCNGC 9 cut(s) 57, 135, 138, 279, 282, 578, 714, 717, 834
GsuI CTGGAG 2 cut(s) 165, 261
HaeIII GGCC 2 cut(s) 921, 1644
HapII CCGG 5 cut(s) 117, 294, 371, 547, 1273
Hin1I GRCGYC 1 cut(s) 1617
Hin1II CATG 8 cut(s) 29, 472, 596, 742, 762, 1375, 1403, 1528
HindIII AAGCTT 2 cut(s) 44, 420
HinfI GANTC 5 cut(s) 1393, 1429, 1465, 1499, 1511
HpaII CCGG 5 cut(s) 117, 294, 371, 547, 1273
HphI GGTGA 5 cut(s) 25, 927, 1003, 1123, 1292
Hpy166II GTNNAC 2 cut(s) 552, 1445
Hpy188I TCNGA 6 cut(s) 343, 855, 954, 1510, 1588, 1663
Hpy188III TCNNGA 4 cut(s) 294, 593, 1034, 1446
Hpy8I GTNNAC 2 cut(s) 552, 1445
Hpy99I CGWCG 1 cut(s) 1619
HpyAV CCTTC 9 cut(s) 23, 605, 694, 932, 977, 1093, 1139, 1170, 1226
HpyCH4III ACNGT 4 cut(s) 168, 347, 1159, 1506
HpyCH4IV ACGT 4 cut(s) 400, 448, 722, 1617
HpyF10VI GCNNNNNNNGC 1 cut(s) 1368
HpyF3I CTNAG 5 cut(s) 74, 777, 1254, 1492, 1599
HpySE526I ACGT 4 cut(s) 400, 448, 722, 1617
Hsp92I GRCGYC 1 cut(s) 1617
Hsp92II CATG 8 cut(s) 29, 472, 596, 742, 762, 1375, 1403, 1528
KflI GGGWCCC 1 cut(s) 868
Kpn2I TCCGGA 1 cut(s) 293
Ksp22I TGATCA 1 cut(s) 978
Kzo9I GATC 7 cut(s) 589, 680, 795, 954, 978, 1306, 1448
LmnI GCTCC 4 cut(s) 242, 785, 1036, 1258
Lsp1109I GCAGC 9 cut(s) 43, 121, 124, 265, 293, 589, 725, 728, 845
LweI GCATC 3 cut(s) 763, 1018, 1104
MaeI CTAG 3 cut(s) 710, 864, 1023
MaeII ACGT 4 cut(s) 400, 448, 722, 1617
MaeIII GTNAC 3 cut(s) 933, 1009, 1500
MalI GATC 7 cut(s) 591, 682, 797, 956, 980, 1308, 1450
MboI GATC 7 cut(s) 589, 680, 795, 954, 978, 1306, 1448
MboII GAAGA 6 cut(s) 295, 422, 1115, 1367, 1496, 1718
MhlI GDGCHC 2 cut(s) 93, 1413
MluCI AATT 9 cut(s) 213, 315, 674, 1057, 1166, 1236, 1487, 1677, 1690
MlyI GAGTC 3 cut(s) 1387, 1459, 1508
Mph1103I ATGCAT 2 cut(s) 31, 760
MroI TCCGGA 1 cut(s) 293
MroXI GAANNNNTTC 5 cut(s) 8, 389, 476, 615, 704
MseI TTAA 4 cut(s) 840, 1061, 1202, 1593
MslI CAYNNNNRTG 3 cut(s) 119, 197, 644
MspI CCGG 5 cut(s) 117, 294, 371, 547, 1273
MspR9I CCNGG 4 cut(s) 365, 371, 1273, 1477
Mva1269I GAATGC 3 cut(s) 29, 126, 944
MvaI CCWGG 2 cut(s) 365, 1477
MwoI GCNNNNNNNGC 1 cut(s) 1368
NciI CCSGG 2 cut(s) 371, 1273
NdeII GATC 7 cut(s) 589, 680, 795, 954, 978, 1306, 1448
NlaIII CATG 8 cut(s) 29, 472, 596, 742, 762, 1375, 1403, 1528
NlaIV GGNNCC 6 cut(s) 369, 787, 869, 870, 879, 1576
NmeAIII GCCGAG 1 cut(s) 1375
NmuCI GTSAC 3 cut(s) 933, 1009, 1500
NsiI ATGCAT 2 cut(s) 31, 760
NspI RCATGY 1 cut(s) 29
PagI TCATGA 1 cut(s) 592
PasI CCCWGGG 1 cut(s) 1476
PctI GAATGC 3 cut(s) 29, 126, 944
PdmI GAANNNNTTC 5 cut(s) 8, 389, 476, 615, 704
PfeI GAWTC 2 cut(s) 1429, 1511
PflFI GACNNNGTC 1 cut(s) 1616
PflMI CCANNNNNTGG 2 cut(s) 401, 572
PfoI TCCNGGA 1 cut(s) 363
PkrI GCNGC 9 cut(s) 58, 136, 139, 280, 283, 579, 715, 718, 835
PleI GAGTC 3 cut(s) 1387, 1459, 1507
PmaCI CACGTG 1 cut(s) 401
PmlI CACGTG 1 cut(s) 401
PpsI GAGTC 3 cut(s) 1387, 1459, 1507
Ppu21I YACGTR 1 cut(s) 401
PpuMI RGGWCCY 2 cut(s) 367, 868
Psp1406I AACGTT 1 cut(s) 722
Psp5II RGGWCCY 2 cut(s) 367, 868
Psp6I CCWGG 2 cut(s) 363, 1475
PspCI CACGTG 1 cut(s) 401
PspGI CCWGG 2 cut(s) 363, 1475
PspN4I GGNNCC 6 cut(s) 369, 787, 869, 870, 879, 1576
PspPI GGNCC 3 cut(s) 367, 868, 1643
PspPPI RGGWCCY 2 cut(s) 367, 868
PstI CTGCAG 1 cut(s) 283
PsyI GACNNNGTC 1 cut(s) 1616
RsaI GTAC 3 cut(s) 551, 860, 1316
RsaNI GTAC 3 cut(s) 550, 859, 1315
RseI CAYNNNNRTG 3 cut(s) 119, 197, 644
SaqAI TTAA 4 cut(s) 840, 1061, 1202, 1593
SatI GCNGC 9 cut(s) 57, 135, 138, 279, 282, 578, 714, 717, 834
Sau3AI GATC 7 cut(s) 589, 680, 795, 954, 978, 1306, 1448
Sau96I GGNCC 3 cut(s) 367, 868, 1643
ScaI AGTACT 1 cut(s) 860
SchI GAGTC 3 cut(s) 1387, 1459, 1508
ScrFI CCNGG 4 cut(s) 365, 371, 1273, 1477
SduI GDGCHC 2 cut(s) 93, 1413
SfaNI GCATC 3 cut(s) 763, 1018, 1104
SfcI CTRYAG 2 cut(s) 279, 1416
SinI GGWCC 2 cut(s) 367, 868
SmiMI CAYNNNNRTG 3 cut(s) 119, 197, 644
SmlI CTYRAG 1 cut(s) 47
SmoI CTYRAG 1 cut(s) 47
Sse9I AATT 9 cut(s) 213, 315, 674, 1057, 1166, 1236, 1487, 1677, 1690
SspMI CTAG 3 cut(s) 710, 864, 1023
StyD4I CCNGG 4 cut(s) 363, 369, 1271, 1475
StyI CCWWGG 1 cut(s) 522
TaaI ACNGT 4 cut(s) 168, 347, 1159, 1506
TaiI ACGT 4 cut(s) 403, 451, 725, 1620
TaqI TCGA 2 cut(s) 570, 894
TasI AATT 9 cut(s) 213, 315, 674, 1057, 1166, 1236, 1487, 1677, 1690
TatI WGTACW 1 cut(s) 858
TfiI GAWTC 2 cut(s) 1429, 1511
Tru1I TTAA 4 cut(s) 840, 1061, 1202, 1593
Tru9I TTAA 4 cut(s) 840, 1061, 1202, 1593
TscAI CASTG 7 cut(s) 352, 690, 1006, 1158, 1316, 1439, 1509
TseFI GTSAC 3 cut(s) 933, 1009, 1500
TseI GCWGC 9 cut(s) 56, 134, 137, 278, 281, 577, 713, 716, 833
Tsp45I GTSAC 3 cut(s) 933, 1009, 1500
TspDTI ATGAA 8 cut(s) 5, 17, 485, 1263, 1299, 1388, 1497, 1719
TspGWI ACGGA 2 cut(s) 821, 1349
TspRI CASTG 7 cut(s) 352, 690, 1006, 1158, 1316, 1439, 1509
Tth111I GACNNNGTC 1 cut(s) 1616
Van91I CCANNNNNTGG 2 cut(s) 401, 572
VpaK11BI GGWCC 2 cut(s) 367, 868
XapI RAATTY 3 cut(s) 674, 1166, 1487
XceI RCATGY 1 cut(s) 29
XmnI GAANNNNTTC 5 cut(s) 8, 389, 476, 615, 704
XspI CTAG 3 cut(s) 710, 864, 1023
ZraI GACGTC 1 cut(s) 1618
ZrmI AGTACT 1 cut(s) 860
Zsp2I ATGCAT 2 cut(s) 31, 760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.