FvH4_1g25930

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000598
Physical Location & Seq
Reverse (-)
17821483 .. 17826900
5418 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g25930.t3

Sequence Viewer

Length: 3261 bp
ATGGCATCGGAAGGCGGGAAGAGCTTTGCGAGGAGAGACCGTCTCCTGGAAATCGAGGCCAAAGCGCGAGCCTGGTGGGACGACAACCATGTCTTCAAGGCCGAAGCTCGTGAGAAGCTTCCAGAACCCGGGGAGAAGTTCTTCGGAAACTTCCCCTTCCCTTACATGAACGGCTTTCTGCATCTCGGACACGCCTTTTCGCTTTCCAAGTTGGAGTTCGCGGCGGCTTATCACCGCTTGAGGGGCGCCAATGTGCTTCTGCCGTTTGCCTTCCACTGCACCGGAATGCCTATCAAGGCCTCTGCCGATAAGCTTGCCAGGGAGATTCAGCTCTTCGGGGAGCCTCCGGTGTTTCCCGCCGCTCAGGAGAAGCAGGAGGAGGCTCAGGAGGCGGAACCGGAAGCGGAGAATGCCAATGGGGGTGCTAAGTACAAGGGGAAGAAGTCCAAGGTTGCGGCAAAGTCCGCCGGACAAGTGTACCAGTGGGAAATTATGCGCAGTTTTGGTCTGTCGGACAGTGAGATATCCAAATTCCAGGATCCTTATAAGTGGTTGACGTTTTTTCCGCCATTGGCAGTGGAGGACCTCAAGGCTTTCGGGCTCGGCTGTGACTGGAGACGCTCGTTTATTACTACAGACATGAACCCTTTTTTCGATGCCTTTGTCAAGTGGCAGGTTAGGAAACTTAAAGACATGGGAAAGATTGTGAAAGATGTTCGTTACGCTGTATACTCTCCATTAGATGGGCAGCCGTGTGCAGATCATGACCGAGCCAGTGGCGAAGGAGTTCAGCCCCAAGAATATACCGTCATTAAAATGGAACTGGTGGGACCTTTTCCTTCTAAATTGGCTGTGTTGGAGGGGAAGAAAGTGTTCCTGGCTGCAGCAACTCTAAGACCTGAGACCATGTACGGCCAAACAAATGCATGGGTATTGCCTGATGGGGAGTATGGAGCCTTTGAAATCAATGAAACTGAGGTATTCATTCTTACGGAAAGAGCAGCTCTTAACCTTGCCTATCAAAACTACTCCAGGATCCCACAGAAACCTACTTGCTTGGTTGAACTTACTGGTCAGGATTTGATTGGCCTTCCATTGAAGTCGCCACTCGCATTGAATCAGATTATTTATGCCCTTCCTATGTTGACTGTTCTAACAGACAAAGGTACTGGGATTGTGACCAGTGTACCCTCTGATTCTCCTGATGATTATATGGCCTTGCATGATTTGAAAAAAAAAGATGCTCTCAGGGAGAAATATGGTGTAAAAAATGAGTGGGTTCTTCCCTTTGATATTATCCCAATCATTGATATTCCAGAATATGGAAATAAAGCTGCAGAAACGGTCTGCGCGAAACTCAATATCAAAAGCCAGAATGAGAAAGAGAAGCTAGCAGAAGCCAAGAAGGAGACCTACTTGAAAGGATTCAATGAAGGAACACTTATTGTGGGGGAATTCAGCGGAAGGAAAGTCCAGGAGGTGAAGCCCCTGATAAGAAGCATGCTTATAGAGGCGGGCGAGGCAATTTCGTACAGTGAGCCTGAGAAGCGGGTAGTATCACGATCAGGGGATGAATGTGTTGTGGCGCTTACAGATCAGTGGTATATCACATACGGGGAAACCGAATGGAAGAAACTGGCTGAGGAGTGCTTGGCCGGTATGAGTTTGTATTCTGATGAAACACGGCATGGGTTTGAACATACTTTGGGCTGGTTAAATCAGTGGGCATGTTCACGATCTTTTGGGCTTGGTACTCGTATTCCCTGGGATGAAGAATTCTTAGTCGAGTCATTGTCTGATTCAACTATTTACATGGCTTATTACACCATCGCTCAGTTCCTCCACAATGGAGATATGTATGGTTCCAGCAAATCCGCAATTAAACCTGAACAAATGACTGATGAGGTCTGGGATTATATCTTCTGTGATGGCCCAGAACCTAAATCATCTGGCATCTCATCATCAACTCTTCGTAATATGAAGCATGAGTTTGAGTATTGGTATCCTTTTGATCTTCGAGTGTCTGGCAAAGATCTCATACAAAATCATTTAACCTTCTGCATCTACAACCATACAGCTATTATGTCCAAGAAGCACTGGCCTCGTGGTTTCAGATGCAATGGGCATATCATGCTTAATGCTGAGAAGATGTCCAAGTCTACAGGGAATTTTAGGACACTTCGTCAGGCAATTGAGGAATTTTCTGCTGATGCTACTCGGTTTTCTTTGGCTGATGCTGGAGACGGTGTTGACGATGCAAATTTTGTATTTGAGACTGCCAATGCTGCAATTCTGCGGCTTACTAAAGAGATCTCATGGATGGAAGAAGTTTTGGCTGCAGAATCTTCCTTGAGACCAGGCCCCCCAACTACTTATGCTGACAAGGTCTTTGCAAATGAGATAAATATTGCTGTGAACAGGACTCAGCAGAATTACAGTGATTACATGTTCCGGGACGCCCTGAAGACTGGATTTTATGATCTTCAAACTGCTAGGGATGAGTACCGCTTTTCGTGTGGTGCTGGGGGAATGAACCATGATTTGGTGTGGAGATTTATGGATGTGCAGACACGTCTAATTACCCCAATCTGTCCACACTATGGAGAATATGTCTGGAGGGAACTGTTGAAGAAGGAAGGGTTTGTGGTAAATGCAGGATGGCCTGTGGCTGATGCTCCAGACCTAACCCTCCAGAGTGCCAATAAGTATTTGCAGGACTCCATCATTTTAATGAGGAAGCTCCTCCAAAAGCAAGTTTTAGGCTCAAAGAAAGGTAACAAGAAGGGTACCTCAGTTACATCGGTGACAGAAGACAACAAGCTAAGCGGCTTGATTTACGTAAATGAGCACTTTGATGGGTGGAAGGCAGAGTGCTTGAAAATACTCCAGATTAACTTTGACAAAGACAGTCGTACCTTTTCTTCAGACATGGTGATACAAGAGGCTCTGCAAAACAGTTCTATTGGTCAGTCAAAAGATTTTAAACAAATCCAAAAGCTTTGTATGCCTTTCATGAAGTTTAAGAAGGATGCGGCAGTTAAACTTGGGCCTCAGGCCTTAGATTTGAAGCTACCTTTTGGCGAGATTGAGGTCCTGCAAGAGAACTTGGACTTAATTAAGAGACAACTTGGTCTTCAAGATGTAGAAGTATTGTCTGGTACAGCTCCTGATGCACTTTCTAAAGCTGGTTCTCTTGTCAAACTAATACAGCAGAATCCTCCAACACCAGGAAACCCCACCGCCATCTTCTTGACCCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

1087

Amino Acids

122.4

Weight (kDa)

5.82

Isoelectric Point (pI)

44.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1 PF00133 24 - 104 6.5e-08 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1 PF00133 194 - 754 3.5e-16 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g PF09334 661 - 760 4.5e-08 tRNA synthetases class I (M)
tRNA-synt_1e PF01406 668 - 744 4.1e-08 tRNA synthetases class I (C) catalytic domain
Anticodon_1 PF08264 794 - 923 1.4e-13 Anticodon-binding domain of tRNA ligase
RBD_LARS1 PF24810 948 - 1024 6e-06 Leucine--tRNA ligase, RagD-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000598)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09620
fragaria_vesca FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_3g34581 FvH4_4g33720 FvH4_4g33720
malus_domestica MD08G1244600.v1.1 MD15G1439400.v1.1
prunus_persica Prupe.1G582000_v2.0.a1 Prupe.2G015100_v2.0.a1 Prupe.2G123100_v2.0.a1
pyrus_communis pycom08g21210 pycom08g21220 pycom15g38730
rosa_chinensis RchiOBHm_Chr1g0336121 RchiOBHm_Chr1g0353811 RchiOBHm_Chr1g0373331 RchiOBHm_Chr4g0443191 RchiOBHm_Chr4g0443201 RchiOBHm_Chr4g0443211
rosa_laevigata RLG00000003122 RLG00000005948 RLG00000026826 RLG00000028251 RLG00000028252 RLG00000028253 RLG00000029377
rosa_multiflora Rmu_co8002498.1_g000001 Rmu_co8262279.1_g000001 Rmu_co8314139.1_g000001 Rmu_co8393711.1_g000001 Rmu_co8419167.1_g000001 Rmu_sc0007968.1_g000004 Rmu_sc0018919.1_g000004 Rmu_sc0027447.1_g000001
rosa_roxburghii Rroxscaffold_4G00284260 Rroxscaffold_4G00301490 Rroxscaffold_4G00315860 Rroxscaffold_5G00383790 Rroxscaffold_5G00383800
rosa_rugosa Rorug01G0123100 Rorug01G0238700 Rorug01G0377100 Rorug01G0377100 Rorug01G0377200 Rorug01G0377300 Rorug04G0344700 Rorug05G0347900.1
rosa_samantha Rh1AG143800 Rh1AG250300 Rh1AG386300 Rh1BG113400 Rh1BG220800 Rh1BG220900 Rh1BG350400 Rh1CG135500 Rh1CG233100 Rh1CG363500 Rh1DG150200 Rh1DG247700 Rh1DG247800 Rh1DG381300 Rh4AG399400 Rh4AG399500 Rh4BG411000 Rh4BG411100 Rh4BG411200 Rh4CG426500 Rh4CG426600 Rh4DG406100 Rh4DG406200 Rh4DG406300
rosa_wichuraiana Rw0G015190 Rw1G021850 Rw1G034870 Rw4G034440 Rw4G034450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 546
AasI GACNNNNNNGTC 2 cut(s) 89, 3129
Acc16I TGCGCA 1 cut(s) 497
Acc36I ACCTGC 1 cut(s) 664
Acc65I GGTACC 1 cut(s) 2786
AccB1I GGYRCC 2 cut(s) 245, 2786
AccB7I CCANNNNNTGG 4 cut(s) 743, 1322, 2542, 2600
AccBSI CCGCTC 1 cut(s) 362
AccI GTMKAC 2 cut(s) 729, 2159
AccII CGCG 3 cut(s) 67, 221, 1352
AclWI GGATC 4 cut(s) 533, 546, 1030, 1043
AcoI YGGCCR 2 cut(s) 913, 1653
AcsI RAATTY 6 cut(s) 530, 1454, 1775, 2167, 2198, 2260
AcuI CTGAAG 2 cut(s) 2483, 2907
AcyI GRCGYC 2 cut(s) 246, 2457
AfiI CCNNNNNNNGG 8 cut(s) 46, 128, 241, 743, 1322, 2542, 2600, 3227
AflIII ACRYGT 2 cut(s) 2445, 2570
AhdI GACNNNNNGTC 1 cut(s) 2181
AjiI CACGTC 1 cut(s) 2573
AjuI GAANNNNNNNTTGG 4 cut(s) 200, 232, 2525, 2557
Alw21I GWGCWC 1 cut(s) 2850
Alw26I GTCTC 9 cut(s) 30, 47, 610, 896, 1403, 2235, 2267, 2347, 3115
AlwI GGATC 4 cut(s) 533, 546, 1030, 1043
AlwNI CAGNNNCTG 1 cut(s) 3167
Ama87I CYCGRG 1 cut(s) 128
ApeKI GCWGC 7 cut(s) 748, 881, 884, 1001, 1334, 2285, 2336
ApoI RAATTY 6 cut(s) 530, 1454, 1775, 2167, 2198, 2260
ArsI GACNNNNNNTTYG 2 cut(s) 2372, 2404
Asp700I GAANNNNTTC 4 cut(s) 140, 786, 872, 1424
Asp718I GGTACC 1 cut(s) 2786
AspLEI GCGC 5 cut(s) 67, 248, 498, 1352, 1588
AspS9I GGNCC 6 cut(s) 583, 830, 1931, 2360, 3047, 3091
AsuC2I CCSGG 4 cut(s) 129, 130, 2453, 3256
AsuHPI GGTGA 4 cut(s) 224, 1492, 2815, 2944
AsuNHI GCTAGC 1 cut(s) 1390
AvaI CYCGRG 1 cut(s) 128
AvaII GGWCC 3 cut(s) 583, 830, 3091
AxyI CCTNAGG 1 cut(s) 3051
BaeI ACNNNNGTAYC 8 cut(s) 461, 494, 1170, 1203, 2778, 2811, 2896, 2929
BamHI GGATCC 2 cut(s) 538, 1035
BanI GGYRCC 2 cut(s) 245, 2786
BanII GRGCYC 1 cut(s) 603
BarI GAAGNNNNNNTAC 4 cut(s) 1397, 1429, 2905, 2937
BauI CACGAG 2 cut(s) 108, 2103
BbsI GAAGAC 4 cut(s) 85, 2471, 2817, 3125
Bbv12I GWGCWC 1 cut(s) 2850
BbvCI CCTCAGC 1 cut(s) 1641
BbvI GCAGC 7 cut(s) 760, 868, 896, 1013, 1321, 2272, 2323
BccI CCATC 9 cut(s) 737, 935, 1835, 1922, 2314, 2652, 2729, 2849, 3251
BceAI ACGGC 5 cut(s) 187, 247, 736, 928, 1700
BcgI CGANNNNNNTGC 4 cut(s) 296, 330, 2084, 2118
BciVI GTATCC 1 cut(s) 2013
BcnI CCSGG 4 cut(s) 129, 130, 2453, 3256
BcoDI GTCTC 9 cut(s) 30, 47, 610, 896, 1403, 2235, 2267, 2347, 3115
BfaI CTAG 2 cut(s) 1391, 2493
BfmI CTRYAG 5 cut(s) 633, 882, 1335, 2160, 2337
BfoI RGCGCY 2 cut(s) 249, 1589
BfuAI ACCTGC 1 cut(s) 664
BfuI GTATCC 1 cut(s) 2013
BglII AGATCT 2 cut(s) 2032, 2310
BlpI GCTNAGC 1 cut(s) 2822
Bme18I GGWCC 3 cut(s) 583, 830, 3091
BmeRI GACNNNNNGTC 1 cut(s) 2181
BmeT110I CYCGRG 1 cut(s) 128
BmgBI CACGTC 1 cut(s) 2573
BmgT120I GGNCC 6 cut(s) 583, 830, 1931, 2360, 3047, 3091
BmrI ACTGGG 1 cut(s) 1179
BmtI GCTAGC 1 cut(s) 1394
BmuI ACTGGG 1 cut(s) 1179
BpiI GAAGAC 4 cut(s) 85, 2471, 2817, 3125
BplI GAGNNNNNCTC 2 cut(s) 27, 59
BpmI CTGGAG 7 cut(s) 634, 1015, 2259, 2635, 2661, 2675, 2870
Bpu10I CCTNAGC 3 cut(s) 363, 384, 1641
Bpu1102I GCTNAGC 1 cut(s) 2822
BpuEI CTTGAG 3 cut(s) 259, 572, 2371
BpuMI CCSGG 4 cut(s) 129, 130, 2453, 3256
BsaAI YACGTR 1 cut(s) 2839
BsaHI GRCGYC 2 cut(s) 246, 2457
BsaI GGTCTC 4 cut(s) 30, 896, 1403, 2347
BsaJI CCNNGG 6 cut(s) 128, 129, 318, 447, 1763, 1764
BsaWI WCCGGW 3 cut(s) 281, 346, 397
BsaXI ACNNNNNCTCC 6 cut(s) 25, 55, 2232, 2262, 2595, 2625
Bsc4I CCNNNNNNNGG 8 cut(s) 46, 128, 241, 743, 1322, 2542, 2600, 3227
Bse118I RCCGGY 1 cut(s) 1655
Bse21I CCTNAGG 1 cut(s) 3051
Bse3DI GCAATG 1 cut(s) 2125
BseDI CCNNGG 6 cut(s) 128, 129, 318, 447, 1763, 1764
BseGI GGATG 7 cut(s) 1576, 1774, 2325, 2503, 2566, 2663, 3034
BseLI CCNNNNNNNGG 8 cut(s) 46, 128, 241, 743, 1322, 2542, 2600, 3227
BseMI GCAATG 1 cut(s) 2125
BseRI GAGGAG 4 cut(s) 46, 392, 1658, 2732
BseXI GCAGC 7 cut(s) 760, 868, 896, 1013, 1321, 2272, 2323
BseYI CCCAGC 1 cut(s) 2522
BsgI GTGCAG 3 cut(s) 262, 777, 2585
Bsh1236I CGCG 3 cut(s) 67, 221, 1352
BshNI GGYRCC 2 cut(s) 245, 2786
BsiHKAI GWGCWC 1 cut(s) 2850
BsiHKCI CYCGRG 1 cut(s) 128
BsiSI CCGG 8 cut(s) 129, 282, 347, 398, 468, 1656, 2452, 3256
BslFI GGGAC 3 cut(s) 92, 843, 2468
BslI CCNNNNNNNGG 8 cut(s) 46, 128, 241, 743, 1322, 2542, 2600, 3227
BsmAI GTCTC 9 cut(s) 30, 47, 610, 896, 1403, 2235, 2267, 2347, 3115
BsmBI CGTCTC 3 cut(s) 47, 610, 2235
BsmFI GGGAC 3 cut(s) 92, 843, 2468
BsmI GAATGC 2 cut(s) 291, 415
Bso31I GGTCTC 4 cut(s) 30, 896, 1403, 2347
BsoBI CYCGRG 1 cut(s) 128
Bsp1286I GDGCHC 2 cut(s) 603, 2850
Bsp1720I GCTNAGC 1 cut(s) 2822
BspFNI CGCG 3 cut(s) 67, 221, 1352
BspHI TCATGA 2 cut(s) 763, 3012
BspMAI CTGCAG 3 cut(s) 886, 1339, 2341
BspMI ACCTGC 1 cut(s) 664
BspOI GCTAGC 1 cut(s) 1394
BspPI GGATC 4 cut(s) 533, 546, 1030, 1043
BspQI GCTCTTC 2 cut(s) 14, 338
BspT107I GGYRCC 2 cut(s) 245, 2786
BspTNI GGTCTC 4 cut(s) 30, 896, 1403, 2347
BsrBI CCGCTC 1 cut(s) 362
BsrDI GCAATG 1 cut(s) 2125
BsrFI RCCGGY 1 cut(s) 1655
BssAI RCCGGY 1 cut(s) 1655
BssECI CCNNGG 6 cut(s) 128, 129, 318, 447, 1763, 1764
BssNAI GTATAC 1 cut(s) 730
BssNI GRCGYC 2 cut(s) 246, 2457
BssSI CACGAG 2 cut(s) 108, 2103
BssT1I CCWWGG 1 cut(s) 447
Bst1107I GTATAC 1 cut(s) 730
Bst2BI CACGAG 2 cut(s) 108, 2103
Bst6I CTCTTC 3 cut(s) 14, 338, 1974
BstACI GRCGYC 2 cut(s) 246, 2457
BstAPI GCANNNNNTGC 1 cut(s) 2131
BstBAI YACGTR 1 cut(s) 2839
BstC8I GCNNGC 5 cut(s) 69, 315, 1392, 1502, 1516
BstF5I GGATG 7 cut(s) 1576, 1774, 2325, 2503, 2566, 2663, 3034
BstFNI CGCG 3 cut(s) 67, 221, 1352
BstH2I RGCGCY 2 cut(s) 249, 1589
BstHHI GCGC 5 cut(s) 67, 248, 498, 1352, 1588
BstMAI GTCTC 9 cut(s) 30, 47, 610, 896, 1403, 2235, 2267, 2347, 3115
BstNSI RCATGY 3 cut(s) 1504, 1731, 2449
BstSFI CTRYAG 5 cut(s) 633, 882, 1335, 2160, 2337
BstSNI TACGTA 1 cut(s) 2839
BstUI CGCG 3 cut(s) 67, 221, 1352
BstV1I GCAGC 7 cut(s) 760, 868, 896, 1013, 1321, 2272, 2323
BstV2I GAAGAC 4 cut(s) 85, 2471, 2817, 3125
BstX2I RGATCY 4 cut(s) 538, 1035, 2032, 2310
BstYI RGATCY 4 cut(s) 538, 1035, 2032, 2310
BstZ17I GTATAC 1 cut(s) 730
Bsu36I CCTNAGG 1 cut(s) 3051
BsuI GTATCC 1 cut(s) 2013
BtgZI GCGATG 1 cut(s) 1813
BtrI CACGTC 1 cut(s) 2573
BtsCI GGATG 7 cut(s) 1576, 1774, 2325, 2503, 2566, 2663, 3034
BtsI GCAGTG 2 cut(s) 274, 582
BveI ACCTGC 1 cut(s) 664
Cac8I GCNNGC 5 cut(s) 69, 315, 1392, 1502, 1516
CaiI CAGNNNCTG 1 cut(s) 3167
CciI TCATGA 2 cut(s) 763, 3012
CfoI GCGC 5 cut(s) 67, 248, 498, 1352, 1588
Cfr10I RCCGGY 1 cut(s) 1655
Cfr13I GGNCC 6 cut(s) 583, 830, 1931, 2360, 3047, 3091
Cfr9I CCCGGG 1 cut(s) 128
CseI GACGC 2 cut(s) 627, 2465
CspCI CAANNNNNGTGG 2 cut(s) 1095, 1130
DinI GGCGCC 1 cut(s) 247
DraI TTTAAA 1 cut(s) 2983
DrdI GACNNNNNNGTC 2 cut(s) 89, 3129
DriI GACNNNNNGTC 1 cut(s) 2181
DseDI GACNNNNNNGTC 2 cut(s) 89, 3129
EaeI YGGCCR 2 cut(s) 913, 1653
Eam1104I CTCTTC 3 cut(s) 14, 338, 1974
Eam1105I GACNNNNNGTC 1 cut(s) 2181
EarI CTCTTC 3 cut(s) 14, 338, 1974
EciI GGCGGA 3 cut(s) 407, 454, 555
Eco105I TACGTA 1 cut(s) 2839
Eco130I CCWWGG 1 cut(s) 447
Eco147I AGGCCT 2 cut(s) 299, 3056
Eco24I GRGCYC 1 cut(s) 603
Eco31I GGTCTC 4 cut(s) 30, 896, 1403, 2347
Eco32I GATATC 1 cut(s) 525
Eco47I GGWCC 3 cut(s) 583, 830, 3091
Eco57I CTGAAG 2 cut(s) 2483, 2907
Eco81I CCTNAGG 1 cut(s) 3051
Eco88I CYCGRG 1 cut(s) 128
EcoO109I RGGNCCY 4 cut(s) 583, 830, 2360, 3091
EcoRI GAATTC 2 cut(s) 1454, 1775
EcoRV GATATC 1 cut(s) 525
EcoT14I CCWWGG 1 cut(s) 447
EcoT22I ATGCAT 1 cut(s) 928
EcoT38I GRGCYC 1 cut(s) 603
EgeI GGCGCC 1 cut(s) 247
EheI GGCGCC 1 cut(s) 247
ErhI CCWWGG 1 cut(s) 447
Esp3I CGTCTC 3 cut(s) 47, 610, 2235
FalI AAGNNNNNCTT 2 cut(s) 1425, 1457
FaqI GGGAC 3 cut(s) 92, 843, 2468
FauI CCCGC 4 cut(s) 8, 364, 1507, 1542
FblI GTMKAC 2 cut(s) 729, 2159
FokI GGATG 7 cut(s) 1583, 1781, 2332, 2510, 2573, 2670, 3041
FriOI GRGCYC 1 cut(s) 603
FspBI CTAG 2 cut(s) 1391, 2493
FspI TGCGCA 1 cut(s) 497
GlaI GCGC 5 cut(s) 66, 247, 497, 1351, 1587
GsaI CCCAGC 1 cut(s) 2526
GsuI CTGGAG 7 cut(s) 634, 1015, 2259, 2635, 2661, 2675, 2870
HaeII RGCGCY 2 cut(s) 249, 1589
HapII CCGG 8 cut(s) 129, 282, 347, 398, 468, 1656, 2452, 3256
HgaI GACGC 2 cut(s) 627, 2465
HhaI GCGC 5 cut(s) 67, 248, 498, 1352, 1588
Hin1I GRCGYC 2 cut(s) 246, 2457
Hin6I GCGC 5 cut(s) 65, 246, 496, 1350, 1586
HinP1I GCGC 5 cut(s) 65, 246, 496, 1350, 1586
HincII GTYRAC 3 cut(s) 555, 1146, 2251
HindII GTYRAC 3 cut(s) 555, 1146, 2251
HindIII AAGCTT 3 cut(s) 116, 311, 2996
HpaII CCGG 8 cut(s) 129, 282, 347, 398, 468, 1656, 2452, 3256
HphI GGTGA 4 cut(s) 224, 1492, 2815, 2944
HpyCH4IV ACGT 3 cut(s) 557, 2572, 2838
HpySE526I ACGT 3 cut(s) 557, 2572, 2838
Hsp92I GRCGYC 2 cut(s) 246, 2457
HspAI GCGC 5 cut(s) 65, 246, 496, 1350, 1586
KasI GGCGCC 1 cut(s) 245
KpnI GGTACC 1 cut(s) 2790
LguI GCTCTTC 2 cut(s) 14, 338
LmnI GCTCC 5 cut(s) 340, 953, 2680, 2745, 3169
Lsp1109I GCAGC 7 cut(s) 760, 868, 896, 1013, 1321, 2272, 2323
MaeI CTAG 2 cut(s) 1391, 2493
MaeII ACGT 3 cut(s) 557, 2572, 2838
MaeIII GTNAC 6 cut(s) 608, 719, 1177, 2774, 2794, 2803
MbiI CCGCTC 1 cut(s) 362
MfeI CAATTG 1 cut(s) 2190
MflI RGATCY 4 cut(s) 538, 1035, 2032, 2310
MhlI GDGCHC 2 cut(s) 603, 2850
Mly113I GGCGCC 1 cut(s) 246
MlyI GAGTC 3 cut(s) 1796, 2416, 2711
MmeI TCCRAC 4 cut(s) 192, 492, 837, 3245
Mph1103I ATGCAT 1 cut(s) 928
MroXI GAANNNNTTC 4 cut(s) 140, 786, 872, 1424
MslI CAYNNNNRTG 4 cut(s) 284, 815, 2729, 2853
MspA1I CMGCKG 1 cut(s) 1461
MspI CCGG 8 cut(s) 129, 282, 347, 398, 468, 1656, 2452, 3256
MunI CAATTG 1 cut(s) 2190
Mva1269I GAATGC 2 cut(s) 291, 415
MvnI CGCG 3 cut(s) 67, 221, 1352
NarI GGCGCC 1 cut(s) 246
NciI CCSGG 4 cut(s) 129, 130, 2453, 3256
NheI GCTAGC 1 cut(s) 1390
NmeAIII GCCGAG 1 cut(s) 582
NmuCI GTSAC 3 cut(s) 608, 1177, 2803
NsbI TGCGCA 1 cut(s) 497
NsiI ATGCAT 1 cut(s) 928
NspI RCATGY 3 cut(s) 1504, 1731, 2449
PacI TTAATTAA 1 cut(s) 3117
PaeI GCATGC 1 cut(s) 1504
PagI TCATGA 2 cut(s) 763, 3012
PasI CCCWGGG 1 cut(s) 1764
PceI AGGCCT 2 cut(s) 299, 3056
PciI ACATGT 1 cut(s) 2445
PciSI GCTCTTC 2 cut(s) 14, 338
PcsI WCGNNNNNNNCGW 3 cut(s) 1349, 1620, 2250
PctI GAATGC 2 cut(s) 291, 415
PdmI GAANNNNTTC 4 cut(s) 140, 786, 872, 1424
PfeI GAWTC 7 cut(s) 325, 1117, 1196, 1425, 1799, 2342, 3214
PflFI GACNNNGTC 1 cut(s) 2384
PflMI CCANNNNNTGG 4 cut(s) 743, 1322, 2542, 2600
PfoI TCCNGGA 5 cut(s) 45, 534, 1031, 1473, 2451
PleI GAGTC 3 cut(s) 1795, 2416, 2711
PluTI GGCGCC 1 cut(s) 249
PpsI GAGTC 3 cut(s) 1795, 2416, 2711
Ppu21I YACGTR 1 cut(s) 2839
PpuMI RGGWCCY 3 cut(s) 583, 830, 3091
PscI ACATGT 1 cut(s) 2445
PsiI TTATAA 1 cut(s) 546
Psp5II RGGWCCY 3 cut(s) 583, 830, 3091
PspFI CCCAGC 1 cut(s) 2522
PspPI GGNCC 6 cut(s) 583, 830, 1931, 2360, 3047, 3091
PspPPI RGGWCCY 3 cut(s) 583, 830, 3091
PstI CTGCAG 3 cut(s) 886, 1339, 2341
PstNI CAGNNNCTG 1 cut(s) 3167
PsuI RGATCY 4 cut(s) 538, 1035, 2032, 2310
PsyI GACNNNGTC 1 cut(s) 2384
RseI CAYNNNNRTG 4 cut(s) 284, 815, 2729, 2853
SapI GCTCTTC 2 cut(s) 14, 338
Sau96I GGNCC 6 cut(s) 583, 830, 1931, 2360, 3047, 3091
SchI GAGTC 3 cut(s) 1796, 2416, 2711
SduI GDGCHC 2 cut(s) 603, 2850
SfcI CTRYAG 5 cut(s) 633, 882, 1335, 2160, 2337
SfoI GGCGCC 1 cut(s) 247
SinI GGWCC 3 cut(s) 583, 830, 3091
SmaI CCCGGG 1 cut(s) 130
SmiMI CAYNNNNRTG 4 cut(s) 284, 815, 2729, 2853
SmlI CTYRAG 3 cut(s) 238, 587, 2350
SmoI CTYRAG 3 cut(s) 238, 587, 2350
SnaBI TACGTA 1 cut(s) 2839
SphI GCATGC 1 cut(s) 1504
SseBI AGGCCT 2 cut(s) 299, 3056
SspDI GGCGCC 1 cut(s) 245
SspI AATATT 1 cut(s) 2407
SspMI CTAG 2 cut(s) 1391, 2493
StuI AGGCCT 2 cut(s) 299, 3056
StyI CCWWGG 1 cut(s) 447
TaiI ACGT 3 cut(s) 560, 2575, 2841
TaqI TCGA 4 cut(s) 54, 654, 1785, 2017
TaqII GACCGA 1 cut(s) 783
TatI WGTACW 1 cut(s) 429
TauI GCSGC 7 cut(s) 224, 227, 362, 458, 2299, 2829, 3035
TfiI GAWTC 7 cut(s) 325, 1117, 1196, 1425, 1799, 2342, 3214
TseFI GTSAC 3 cut(s) 608, 1177, 2803
TseI GCWGC 7 cut(s) 748, 881, 884, 1001, 1334, 2285, 2336
Tsp45I GTSAC 3 cut(s) 608, 1177, 2803
TspGWI ACGGA 1 cut(s) 1007
TspMI CCCGGG 1 cut(s) 128
Tth111I GACNNNGTC 1 cut(s) 2384
Van91I CCANNNNNTGG 4 cut(s) 743, 1322, 2542, 2600
VpaK11BI GGWCC 3 cut(s) 583, 830, 3091
XapI RAATTY 6 cut(s) 530, 1454, 1775, 2167, 2198, 2260
XceI RCATGY 3 cut(s) 1504, 1731, 2449
XmaI CCCGGG 1 cut(s) 128
XmiI GTMKAC 2 cut(s) 729, 2159
XmnI GAANNNNTTC 4 cut(s) 140, 786, 872, 1424
XspI CTAG 2 cut(s) 1391, 2493
Zsp2I ATGCAT 1 cut(s) 928
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.