pycom15g38730

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000598
Physical Location & Seq
Forward (+)
38492000 .. 38495492
3493 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g38730.1

Sequence Viewer

Length: 3273 bp
ATGGCAGCTGAAGGTGGTGGGAGGAGCTTCGCCAGGAGAGACCACCTCCTTGAGATTGAGACAAAGGTTCGAAGCTGGTGGGAAGAGAAGGATGTTTTCAGAGCTGAATCTTGTGAAAAGCCTCCCGAACCTGGGGAGAAGTTCTTTGGAAACTTTCCATTTCCGTACATGAATGGTTTTTTGCATCTTGGACATGCATTCTCACTCTCCAAGCTAGAGTTTGCTGCAGCTTACCATAGGTTAAGAGGTGCCAATGTGCTTTTGCCATTTGCTTTCCATTGCACTGGCATGCCTATCAAAGCGTCGGCTGATAAACTTGCGAGAGAGATTCAACTGTTTGGCGACCCTCCAGTTTTTCCAAAAGAAGTGGAACAGGGGAGTCAGGAAGTTGAAGCAGAAACAGAAGGTGCAAATAGTGGAGCACCTCCAGATAAGTTCAAGGGAAAGAAGTCAAAGGCTGCATCAAAATCAGGTGGACAAGCATACCAATGGGAAATCATGCGTAGTTTTGGCCTCTCCGATAGCGAGATATCCAAATTCCAAAATCCATACAACTGGTTGACATTCTTTCCTCCATTGGCAGTGAAAGACCTCAAGGCTTTTGGATTGGGATGTGATTGGAGGCGGTCATTTATTACTACAGACATGAACCCAGTTTTTGATGCCTTTGTGAGGTGGCAGGTGAGGAAATTAAAATCCATGGGCAAGATTGTGAAAGATGTCCGTTACACAATTTACTCTCCATTGGATGGGCAGCCATGTGCAGATCATGATAGGGCAAGTGGCGAAGGTGTTCAGCCCCAGGAATACATCCTCATCAAAATGGAGGTGGTTGCACCTTTCCCATCTAAACTGAAAGTGTTGGAGGGAAGGAAAGTGTTCCTTGCTGCAGCAACACTGAGACCTGAGACCATGTATGGACAAACAAATGCATGGGTATTGCCTGATGGGAAATATGGAGCCTTTGAAATCAATGAAACAGAGGTACTTATTCTAACGCAGAGAGCAGCACTTAATCTTGCCTATCAGAAGTATTCTAGGGTCCCAGAGAAACCTACTTGTTTGGTGGAGCTCACGGGCCGTGATTTGATTGGCCTTCCATTGAAGTCACCACGTGCAATCAATCAGATTATTTACACTCTTCCTATGTTGACTGTCCTGACAGACAAAGGTACTGGGATTGTGACCAGTGTGCCTGCTGATTCTCCTGATGATTATATGGCCTTACATGATTTAAAAGCCAAACCCGCTCTTAGAGCAAAATATGGCGTGAAGGATGAGTGGGTCATGCCCTTTGAGATTATTCCAATTATCAATATTCCTGAATTTGGAAATAAAGCTGCGGAAAAGGTCTGTGCAGATCTACAAATCAAGAGCCAGAATGAGAAAGAGAAGCTAGCAGAAGCCAAGAGGCTGACTTACCTGAAAGGATTCACTGAAGGAACACTTATTGTGGGTGAATTCGAAGGATGGAAAGTCCAGGAGGCAAAACCGTTGATAAGGAGCAAGCTCATAGAGGCAAATGAAGCAATTGTATATAGTGAACCTGAGAAGCGGGTGGTGTCACGATCAGGTGATGAATGTGTTGTGGCTCTCACAGATCAATGGTATATCACATATGGGGAACCCGAATGGAAAAAACTGGCTGAGGAGTGCTTGTCCAGCATGAATTTGTATCATGACGAGACAAGACATGGATTTGAACACACATTGGGTTGGTTGAATCAATGGGCTTGTTCACGATCTTTTGGGCTTGGGACTCGTATTCCCTGGGATGAAGAGTTCTTAGTTGAGTCGTTATCTGATTCAACTATTTACATGGCTTATTACACTATTGCTCACATTTTACACAATGGAGACATGTATGGTTCCAGCAAATCTGTAATTAAACCTGAACAAATGACTGATGAGGTCTGGGAGTATATTTTCTGTGATGGCCCATATCCTGAATCATCTGACATCTCGTCATCCATTCTTAATAAGATGAAGCAGGAATTTGAGTATTGGTATCCTTTTGATCATCAAGTGTCTGGCATCGATCTAATCCAAAATCATTTAACCTTCTGCATTTACAACCACACGGCAATTATGCGCAAGAAGCACTGGCCTCGTGGGTTTAGATGCAATGGGCACATCATGTTAAATGCTGAGAAGATGTCTAAGTCTACAGGGAACTTTAGGACCATTCGCCAGGCAATTGAGGAATTTTCTGCTGATGCCACGCGATTCTCATTGGCTGATGCTGGAGATGGTGTTGATGATGCAAATTTCGTATTTGAGACTGCAAATGCTGCTATTCTAAAGCTAACTAAAGAGATCGCATGGATGGAAGAAGTTTTAGCTGCAGAATCCTCTTTGAGAATAGGTCCTCCTTCTACTTATGCTGACAGGGTCTTTTTGAATGAGATAAATATTGCTGTGAACAGGACTGAACTGGGTTTAATCGATCTTCAGGCTTCTAGGGATGAGTACAGGCTTTCATGTGGTTCTGGGGGCATGAACCATGAATTGCTGTGGCGTTTTATGGATGTGCAGACACGTCTCATTACCCCAATCTGTCCGCACTATGCCGAATATGTATGGAGGGAACTTTTAAAGAAGGAAGGGTTTGTGGTTAATGCAGGATGGCCTGTGGCTGATTCTCCAGATCTGATCCTCCAGAGTGCCAACAAGTATTTGCAACATTCCATTGTTGTTATGAGGAAGCTTCTTGAAAAGCAAACTTCAGGCTCAACAAAAGCCAATAAGAAGGGTCCTCCAGTTAAAACCTCGATGGAAACCAAAAGGTTGATTGGTTTGATATATGTAAATGAGCAGTTCGATGGATGGAAGGCGGAGTGTTTGAGGATACTACAAAGTAACTTTGACCGAGATACTCGCACTTTTGCTTCAGACAAGGTAATTCTGGAGGCTTTGCAAAGAAGTTCAATTGGTCAGAGTAAAGATTTTAGACAAACCCAAAAGCTTTGTATGCCTTTTTTGAGGTTCAAAAAGGATGAGGCGGTTACTCTTGGGGTCCATGCCTTAGACTTGAAGCTACCATTTGGAGAGATTGAGGTCCTTCAAGAGAACTTGGACTTAATTAAGAGACAAGTGAAGGTGGACACTAAGGGACAAGTCGAGCTTGAAGAAGTAGAAGTTCTGTCTGGTACCGACCCTGATGCTCTTGCTAAAGCTGGTTCACTTGTTAAATTAATAGAGCAGAATCCGCCATCCCCTGGAAGCCCAACTGCCATCTTCTTGACCCGGGGAAAATTACTTTATATAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

1091

Amino Acids

123.25

Weight (kDa)

6.12

Isoelectric Point (pI)

47.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1 PF00133 24 - 105 1.7e-08 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1 PF00133 196 - 756 1.6e-15 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g PF09334 662 - 763 1.6e-07 tRNA synthetases class I (M)
tRNA-synt_1e PF01406 671 - 746 7.5e-08 tRNA synthetases class I (C) catalytic domain
Anticodon_1 PF08264 836 - 907 3.7e-09 Anticodon-binding domain of tRNA ligase
RBD_LARS1 PF24810 937 - 1013 8.9e-07 Leucine--tRNA ligase, RagD-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000598)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09620
fragaria_vesca FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_3g34581 FvH4_4g33720 FvH4_4g33720
malus_domestica MD08G1244600.v1.1 MD15G1439400.v1.1
prunus_persica Prupe.1G582000_v2.0.a1 Prupe.2G015100_v2.0.a1 Prupe.2G123100_v2.0.a1
pyrus_communis pycom08g21210 pycom08g21220 pycom15g38730
rosa_chinensis RchiOBHm_Chr1g0336121 RchiOBHm_Chr1g0353811 RchiOBHm_Chr1g0373331 RchiOBHm_Chr4g0443191 RchiOBHm_Chr4g0443201 RchiOBHm_Chr4g0443211
rosa_laevigata RLG00000003122 RLG00000005948 RLG00000026826 RLG00000028251 RLG00000028252 RLG00000028253 RLG00000029377
rosa_multiflora Rmu_co8002498.1_g000001 Rmu_co8262279.1_g000001 Rmu_co8314139.1_g000001 Rmu_co8393711.1_g000001 Rmu_co8419167.1_g000001 Rmu_sc0007968.1_g000004 Rmu_sc0018919.1_g000004 Rmu_sc0027447.1_g000001
rosa_roxburghii Rroxscaffold_4G00284260 Rroxscaffold_4G00301490 Rroxscaffold_4G00315860 Rroxscaffold_5G00383790 Rroxscaffold_5G00383800
rosa_rugosa Rorug01G0123100 Rorug01G0238700 Rorug01G0377100 Rorug01G0377100 Rorug01G0377200 Rorug01G0377300 Rorug04G0344700 Rorug05G0347900.1
rosa_samantha Rh1AG143800 Rh1AG250300 Rh1AG386300 Rh1BG113400 Rh1BG220800 Rh1BG220900 Rh1BG350400 Rh1CG135500 Rh1CG233100 Rh1CG363500 Rh1DG150200 Rh1DG247700 Rh1DG247800 Rh1DG381300 Rh4AG399400 Rh4AG399500 Rh4BG411000 Rh4BG411100 Rh4BG411200 Rh4CG426500 Rh4CG426600 Rh4DG406100 Rh4DG406200 Rh4DG406300
rosa_wichuraiana Rw0G015190 Rw1G021850 Rw1G034870 Rw4G034440 Rw4G034450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 670
Acc16I TGCGCA 1 cut(s) 2093
Acc36I ACCTGC 1 cut(s) 670
Acc65I GGTACC 1 cut(s) 3149
AccB1I GGYRCC 2 cut(s) 248, 3149
AccB7I CCANNNNNTGG 2 cut(s) 749, 3218
AccBSI CCGCTC 1 cut(s) 1250
AccI GTMKAC 1 cut(s) 2165
AccII CGCG 1 cut(s) 2224
AciI CCGC 8 cut(s) 625, 1248, 1343, 1555, 2561, 2834, 3002, 3209
AclWI GGATC 1 cut(s) 2647
AcsI RAATTY 7 cut(s) 536, 1325, 1460, 1669, 1994, 2204, 2266
AcuI CTGAAG 5 cut(s) 30, 1458, 2435, 2709, 2874
AcvI CACGTG 1 cut(s) 1115
AdeI CACNNNGTG 1 cut(s) 1115
AfaI GTAC 5 cut(s) 167, 987, 1174, 2471, 3151
AfiI CCNNNNNNNGG 6 cut(s) 131, 132, 672, 749, 1328, 3218
AflIII ACRYGT 2 cut(s) 1860, 2537
AhdI GACNNNNNGTC 1 cut(s) 1963
AjiI CACGTC 1 cut(s) 2540
AjnI CCWGG 7 cut(s) 32, 130, 801, 1479, 1769, 2190, 3217
AloI GAACNNNNNNTCC 2 cut(s) 1766, 1798
Alw21I GWGCWC 2 cut(s) 424, 1074
Alw26I GTCTC 9 cut(s) 33, 53, 895, 902, 1679, 1851, 2273, 2546, 3082
AlwI GGATC 1 cut(s) 2647
Ama87I CYCGRG 1 cut(s) 3246
AoxI GGCC 7 cut(s) 511, 1078, 1093, 1221, 1936, 2105, 2627
ApoI RAATTY 7 cut(s) 536, 1325, 1460, 1669, 1994, 2204, 2266
AseI ATTAAT 1 cut(s) 3194
Asp700I GAANNNNTTC 3 cut(s) 792, 878, 1430
Asp718I GGTACC 1 cut(s) 3149
AspLEI GCGC 1 cut(s) 2094
AspS9I GGNCC 8 cut(s) 1042, 1078, 1937, 2181, 2366, 2753, 3016, 3058
AsuC2I CCSGG 2 cut(s) 3247, 3248
AsuHPI GGTGA 4 cut(s) 694, 1101, 1469, 1586
AsuII TTCGAA 2 cut(s) 70, 1464
AsuNHI GCTAGC 1 cut(s) 1396
AvaI CYCGRG 1 cut(s) 3246
AvaII GGWCC 6 cut(s) 1042, 2181, 2366, 2753, 3016, 3058
BaeGI GKGCMC 1 cut(s) 2133
BanI GGYRCC 2 cut(s) 248, 3149
BanII GRGCYC 1 cut(s) 1074
BauI CACGAG 1 cut(s) 2109
BbrPI CACGTG 1 cut(s) 1115
Bbv12I GWGCWC 2 cut(s) 424, 1074
BbvCI CCTCAGC 1 cut(s) 1647
BceAI ACGGC 2 cut(s) 1065, 2097
BcgI CGANNNNNNTGC 4 cut(s) 2090, 2124, 3143, 3177
BciT130I CCWGG 7 cut(s) 34, 132, 803, 1481, 1771, 2192, 3219
BciVI GTATCC 2 cut(s) 2019, 2841
BclI TGATCA 1 cut(s) 2017
BcnI CCSGG 2 cut(s) 3247, 3248
BcoDI GTCTC 9 cut(s) 33, 53, 895, 902, 1679, 1851, 2273, 2546, 3082
BfaI CTAG 4 cut(s) 215, 1038, 1397, 2460
BfmI CTRYAG 5 cut(s) 225, 639, 888, 2166, 2343
BfuAI ACCTGC 1 cut(s) 670
BfuI GTATCC 2 cut(s) 2019, 2841
BglII AGATCT 2 cut(s) 1360, 2647
Bme1390I CCNGG 9 cut(s) 34, 132, 803, 1481, 1771, 2192, 3219, 3247, 3248
Bme18I GGWCC 6 cut(s) 1042, 2181, 2366, 2753, 3016, 3058
BmeRI GACNNNNNGTC 1 cut(s) 1963
BmeT110I CYCGRG 1 cut(s) 3246
BmgBI CACGTC 1 cut(s) 2540
BmgT120I GGNCC 8 cut(s) 1042, 1078, 1937, 2181, 2366, 2753, 3016, 3058
BmiI GGNNCC 9 cut(s) 250, 961, 1043, 1044, 1626, 1870, 2754, 3017, 3151
BmrFI CCNGG 9 cut(s) 34, 132, 803, 1481, 1771, 2192, 3219, 3247, 3248
BmrI ACTGGG 3 cut(s) 647, 1185, 2444
BmsI GCATC 9 cut(s) 193, 470, 652, 2043, 2111, 2206, 2230, 2251, 3151
BmtI GCTAGC 1 cut(s) 1400
BmuI ACTGGG 3 cut(s) 647, 1185, 2444
BplI GAGNNNNNCTC 2 cut(s) 30, 62
BpmI CTGGAG 7 cut(s) 333, 411, 2265, 2628, 2642, 2742, 2927
Bpu10I CCTNAGC 1 cut(s) 1647
Bpu14I TTCGAA 2 cut(s) 70, 1464
BpuEI CTTGAG 2 cut(s) 71, 578
BpuMI CCSGG 2 cut(s) 3247, 3248
Bsa29I ATCGAT 2 cut(s) 2037, 2445
BsaAI YACGTR 1 cut(s) 1115
BsaI GGTCTC 3 cut(s) 33, 895, 902
BsaJI CCNNGG 8 cut(s) 131, 699, 801, 1769, 1770, 3217, 3246, 3247
BsaXI ACNNNNNCTCC 4 cut(s) 1643, 1673, 2238, 2268
Bsc4I CCNNNNNNNGG 6 cut(s) 131, 132, 672, 749, 1328, 3218
Bse3DI GCAATG 2 cut(s) 277, 2131
BseBI CCWGG 7 cut(s) 34, 132, 803, 1481, 1771, 2192, 3219
BseCI ATCGAT 2 cut(s) 2037, 2445
BseDI CCNNGG 8 cut(s) 131, 699, 801, 1769, 1770, 3217, 3246, 3247
BseLI CCNNNNNNNGG 6 cut(s) 131, 132, 672, 749, 1328, 3218
BseMI GCAATG 2 cut(s) 277, 2131
BseMII CTCAG 5 cut(s) 890, 897, 1539, 1638, 2139
BseRI GAGGAG 2 cut(s) 37, 1664
BseSI GKGCMC 1 cut(s) 2133
BsgI GTGCAG 3 cut(s) 783, 1377, 2552
Bsh1236I CGCG 1 cut(s) 2224
BshFI GGCC 7 cut(s) 513, 1080, 1095, 1223, 1938, 2107, 2629
BshNI GGYRCC 2 cut(s) 248, 3149
BshVI ATCGAT 2 cut(s) 2037, 2445
BsiHKAI GWGCWC 2 cut(s) 424, 1074
BsiHKCI CYCGRG 1 cut(s) 3246
BsiSI CCGG 1 cut(s) 3247
BslFI GGGAC 3 cut(s) 1028, 1771, 3126
BslI CCNNNNNNNGG 6 cut(s) 131, 132, 672, 749, 1328, 3218
BsmAI GTCTC 9 cut(s) 33, 53, 895, 902, 1679, 1851, 2273, 2546, 3082
BsmBI CGTCTC 1 cut(s) 2546
BsmFI GGGAC 3 cut(s) 1028, 1771, 3126
BsmI GAATGC 1 cut(s) 197
BsnI GGCC 7 cut(s) 513, 1080, 1095, 1223, 1938, 2107, 2629
Bso31I GGTCTC 3 cut(s) 33, 895, 902
BsoBI CYCGRG 1 cut(s) 3246
Bsp119I TTCGAA 2 cut(s) 70, 1464
Bsp1286I GDGCHC 3 cut(s) 424, 1074, 2133
Bsp19I CCATGG 1 cut(s) 699
BspACI CCGC 8 cut(s) 625, 1248, 1343, 1555, 2561, 2834, 3002, 3209
BspANI GGCC 7 cut(s) 513, 1080, 1095, 1223, 1938, 2107, 2629
BspCNI CTCAG 5 cut(s) 891, 898, 1540, 1639, 2140
BspDI ATCGAT 2 cut(s) 2037, 2445
BspFNI CGCG 1 cut(s) 2224
BspHI TCATGA 2 cut(s) 769, 1678
BspLI GGNNCC 9 cut(s) 250, 961, 1043, 1044, 1626, 1870, 2754, 3017, 3151
BspMAI CTGCAG 3 cut(s) 229, 892, 2347
BspMI ACCTGC 1 cut(s) 670
BspOI GCTAGC 1 cut(s) 1400
BspPI GGATC 1 cut(s) 2647
BspT104I TTCGAA 2 cut(s) 70, 1464
BspT107I GGYRCC 2 cut(s) 248, 3149
BspTNI GGTCTC 3 cut(s) 33, 895, 902
BsrBI CCGCTC 1 cut(s) 1250
BsrDI GCAATG 2 cut(s) 277, 2131
BssECI CCNNGG 8 cut(s) 131, 699, 801, 1769, 1770, 3217, 3246, 3247
BssSI CACGAG 1 cut(s) 2109
BssT1I CCWWGG 1 cut(s) 699
Bst2BI CACGAG 1 cut(s) 2109
Bst2UI CCWGG 7 cut(s) 34, 132, 803, 1481, 1771, 2192, 3219
Bst4CI ACNGT 3 cut(s) 336, 1156, 1494
Bst6I CTCTTC 3 cut(s) 78, 1146, 1773
BstAPI GCANNNNNTGC 1 cut(s) 2291
BstBAI YACGTR 1 cut(s) 1115
BstBI TTCGAA 2 cut(s) 70, 1464
BstC8I GCNNGC 4 cut(s) 290, 1197, 1398, 1508
BstDSI CCRYGG 1 cut(s) 699
BstENI CCTNNNNNAGG 1 cut(s) 670
BstFNI CGCG 1 cut(s) 2224
BstHHI GCGC 1 cut(s) 2094
BstMAI GTCTC 9 cut(s) 33, 53, 895, 902, 1679, 1851, 2273, 2546, 3082
BstMWI GCNNNNNNNGC 8 cut(s) 1247, 1256, 1526, 1662, 2098, 2291, 2971, 3208
BstNI CCWGG 7 cut(s) 34, 132, 803, 1481, 1771, 2192, 3219
BstNSI RCATGY 3 cut(s) 197, 292, 1864
BstSCI CCNGG 9 cut(s) 32, 130, 801, 1479, 1769, 2190, 3217, 3245, 3246
BstSFI CTRYAG 5 cut(s) 225, 639, 888, 2166, 2343
BstSLI GKGCMC 1 cut(s) 2133
BstUI CGCG 1 cut(s) 2224
BstX2I RGATCY 2 cut(s) 1360, 2647
BstXI CCANNNNNNTGG 2 cut(s) 284, 555
BstYI RGATCY 2 cut(s) 1360, 2647
Bsu15I ATCGAT 2 cut(s) 2037, 2445
BsuI GTATCC 2 cut(s) 2019, 2841
BsuRI GGCC 7 cut(s) 513, 1080, 1095, 1223, 1938, 2107, 2629
BsuTUI ATCGAT 2 cut(s) 2037, 2445
BtgI CCRYGG 1 cut(s) 699
BtrI CACGTC 1 cut(s) 2540
BtsI GCAGTG 1 cut(s) 588
BtsIMutI CAGTG 6 cut(s) 282, 588, 896, 1195, 1434, 2101
BveI ACCTGC 1 cut(s) 670
Cac8I GCNNGC 4 cut(s) 290, 1197, 1398, 1508
CciI TCATGA 2 cut(s) 769, 1678
CfoI GCGC 1 cut(s) 2094
Cfr13I GGNCC 8 cut(s) 1042, 1078, 1937, 2181, 2366, 2753, 3016, 3058
Cfr9I CCCGGG 1 cut(s) 3246
ClaI ATCGAT 2 cut(s) 2037, 2445
CseI GACGC 1 cut(s) 291
Csp6I GTAC 5 cut(s) 166, 986, 1173, 2470, 3150
CspCI CAANNNNNGTGG 2 cut(s) 348, 383
CviQI GTAC 5 cut(s) 166, 986, 1173, 2470, 3150
DraI TTTAAA 2 cut(s) 1236, 2595
DraIII CACNNNGTG 1 cut(s) 1115
DriI GACNNNNNGTC 1 cut(s) 1963
Eam1104I CTCTTC 3 cut(s) 78, 1146, 1773
Eam1105I GACNNNNNGTC 1 cut(s) 1963
EarI CTCTTC 3 cut(s) 78, 1146, 1773
EciI GGCGGA 2 cut(s) 2849, 3198
Ecl136II GAGCTC 1 cut(s) 1072
Eco130I CCWWGG 1 cut(s) 699
Eco24I GRGCYC 1 cut(s) 1074
Eco31I GGTCTC 3 cut(s) 33, 895, 902
Eco32I GATATC 1 cut(s) 531
Eco47I GGWCC 6 cut(s) 1042, 2181, 2366, 2753, 3016, 3058
Eco53kI GAGCTC 1 cut(s) 1072
Eco57I CTGAAG 5 cut(s) 30, 1458, 2435, 2709, 2874
Eco72I CACGTG 1 cut(s) 1115
Eco88I CYCGRG 1 cut(s) 3246
EcoICRI GAGCTC 1 cut(s) 1072
EcoNI CCTNNNNNAGG 1 cut(s) 670
EcoO109I RGGNCCY 4 cut(s) 1042, 2366, 2753, 3058
EcoRI GAATTC 1 cut(s) 1460
EcoRII CCWGG 7 cut(s) 32, 130, 801, 1479, 1769, 2190, 3217
EcoRV GATATC 1 cut(s) 531
EcoT14I CCWWGG 1 cut(s) 699
EcoT22I ATGCAT 2 cut(s) 199, 934
EcoT38I GRGCYC 1 cut(s) 1074
ErhI CCWWGG 1 cut(s) 699
Esp3I CGTCTC 1 cut(s) 2546
FalI AAGNNNNNCTT 6 cut(s) 867, 899, 1431, 1463, 3108, 3140
FaqI GGGAC 3 cut(s) 1028, 1771, 3126
FauI CCCGC 2 cut(s) 1255, 1548
FauNDI CATATG 1 cut(s) 1618
FbaI TGATCA 1 cut(s) 2017
FblI GTMKAC 1 cut(s) 2165
FriOI GRGCYC 1 cut(s) 1074
FspBI CTAG 4 cut(s) 215, 1038, 1397, 2460
FspI TGCGCA 1 cut(s) 2093
GlaI GCGC 1 cut(s) 2093
GsuI CTGGAG 7 cut(s) 333, 411, 2265, 2628, 2642, 2742, 2927
HaeIII GGCC 7 cut(s) 513, 1080, 1095, 1223, 1938, 2107, 2629
HapII CCGG 1 cut(s) 3247
HgaI GACGC 1 cut(s) 291
HhaI GCGC 1 cut(s) 2094
Hin6I GCGC 1 cut(s) 2092
HinP1I GCGC 1 cut(s) 2092
HincII GTYRAC 2 cut(s) 561, 1152
HindII GTYRAC 2 cut(s) 561, 1152
HindIII AAGCTT 2 cut(s) 2705, 2963
HpaII CCGG 1 cut(s) 3247
HphI GGTGA 4 cut(s) 694, 1101, 1469, 1586
Hpy166II GTNNAC 9 cut(s) 476, 561, 1152, 1544, 1739, 2166, 2422, 3103, 3182
Hpy188I TCNGA 9 cut(s) 101, 520, 1029, 1128, 1804, 1957, 2652, 2893, 2937
Hpy8I GTNNAC 9 cut(s) 476, 561, 1152, 1544, 1739, 2166, 2422, 3103, 3182
Hpy99I CGWCG 1 cut(s) 307
HpyCH4III ACNGT 3 cut(s) 336, 1156, 1494
HpyCH4IV ACGT 2 cut(s) 1114, 2539
HpyF10VI GCNNNNNNNGC 8 cut(s) 1247, 1256, 1526, 1662, 2098, 2291, 2971, 3208
HpySE526I ACGT 2 cut(s) 1114, 2539
HspAI GCGC 1 cut(s) 2092
KflI GGGWCCC 1 cut(s) 1042
KpnI GGTACC 1 cut(s) 3153
Ksp22I TGATCA 1 cut(s) 2017
LmnI GCTCC 5 cut(s) 24, 419, 959, 1069, 1503
LweI GCATC 9 cut(s) 193, 470, 652, 2043, 2111, 2206, 2230, 2251, 3151
MaeI CTAG 4 cut(s) 215, 1038, 1397, 2460
MaeII ACGT 2 cut(s) 1114, 2539
MaeIII GTNAC 6 cut(s) 725, 1107, 1183, 1563, 2858, 3004
MbiI CCGCTC 1 cut(s) 1250
MboII GAAGA 8 cut(s) 95, 1133, 1790, 2164, 2342, 2441, 3140, 3229
MfeI CAATTG 3 cut(s) 1530, 2196, 2928
MflI RGATCY 2 cut(s) 1360, 2647
MhlI GDGCHC 3 cut(s) 424, 1074, 2133
MlyI GAGTC 3 cut(s) 388, 1753, 1802
MmeI TCCRAC 1 cut(s) 843
Mph1103I ATGCAT 2 cut(s) 199, 934
MroXI GAANNNNTTC 3 cut(s) 792, 878, 1430
MslI CAYNNNNRTG 3 cut(s) 287, 487, 821
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 1 cut(s) 3247
MspR9I CCNGG 9 cut(s) 34, 132, 803, 1481, 1771, 2192, 3219, 3247, 3248
MunI CAATTG 3 cut(s) 1530, 2196, 2928
Mva1269I GAATGC 1 cut(s) 197
MvaI CCWGG 7 cut(s) 34, 132, 803, 1481, 1771, 2192, 3219
MvnI CGCG 1 cut(s) 2224
MwoI GCNNNNNNNGC 8 cut(s) 1247, 1256, 1526, 1662, 2098, 2291, 2971, 3208
NciI CCSGG 2 cut(s) 3247, 3248
NcoI CCATGG 1 cut(s) 699
NdeI CATATG 1 cut(s) 1618
NheI GCTAGC 1 cut(s) 1396
NlaIV GGNNCC 9 cut(s) 250, 961, 1043, 1044, 1626, 1870, 2754, 3017, 3151
NmuCI GTSAC 3 cut(s) 1107, 1183, 1563
NsbI TGCGCA 1 cut(s) 2093
NsiI ATGCAT 2 cut(s) 199, 934
NspI RCATGY 3 cut(s) 197, 292, 1864
NspV TTCGAA 2 cut(s) 70, 1464
PacI TTAATTAA 1 cut(s) 3084
PaeI GCATGC 1 cut(s) 292
PagI TCATGA 2 cut(s) 769, 1678
PaqCI CACCTGC 1 cut(s) 670
PasI CCCWGGG 1 cut(s) 1770
PciI ACATGT 1 cut(s) 1860
PctI GAATGC 1 cut(s) 197
PdmI GAANNNNTTC 3 cut(s) 792, 878, 1430
PflFI GACNNNGTC 1 cut(s) 2390
PflMI CCANNNNNTGG 2 cut(s) 749, 3218
PfoI TCCNGGA 1 cut(s) 1479
PleI GAGTC 3 cut(s) 387, 1753, 1801
PmaCI CACGTG 1 cut(s) 1115
PmlI CACGTG 1 cut(s) 1115
PpsI GAGTC 3 cut(s) 387, 1753, 1801
Ppu21I YACGTR 1 cut(s) 1115
PpuMI RGGWCCY 4 cut(s) 1042, 2366, 2753, 3058
PscI ACATGT 1 cut(s) 1860
PshBI ATTAAT 1 cut(s) 3194
Psp124BI GAGCTC 1 cut(s) 1074
Psp5II RGGWCCY 4 cut(s) 1042, 2366, 2753, 3058
Psp6I CCWGG 7 cut(s) 32, 130, 801, 1479, 1769, 2190, 3217
PspCI CACGTG 1 cut(s) 1115
PspGI CCWGG 7 cut(s) 32, 130, 801, 1479, 1769, 2190, 3217
PspN4I GGNNCC 9 cut(s) 250, 961, 1043, 1044, 1626, 1870, 2754, 3017, 3151
PspPI GGNCC 8 cut(s) 1042, 1078, 1937, 2181, 2366, 2753, 3016, 3058
PspPPI RGGWCCY 4 cut(s) 1042, 2366, 2753, 3058
PstI CTGCAG 3 cut(s) 229, 892, 2347
PsuI RGATCY 2 cut(s) 1360, 2647
PsyI GACNNNGTC 1 cut(s) 2390
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 5 cut(s) 167, 987, 1174, 2471, 3151
RsaNI GTAC 5 cut(s) 166, 986, 1173, 2470, 3150
RseI CAYNNNNRTG 3 cut(s) 287, 487, 821
SacI GAGCTC 1 cut(s) 1074
Sau96I GGNCC 8 cut(s) 1042, 1078, 1937, 2181, 2366, 2753, 3016, 3058
SchI GAGTC 3 cut(s) 388, 1753, 1802
ScrFI CCNGG 9 cut(s) 34, 132, 803, 1481, 1771, 2192, 3219, 3247, 3248
SduI GDGCHC 3 cut(s) 424, 1074, 2133
SfaNI GCATC 9 cut(s) 193, 470, 652, 2043, 2111, 2206, 2230, 2251, 3151
SfcI CTRYAG 5 cut(s) 225, 639, 888, 2166, 2343
SfuI TTCGAA 2 cut(s) 70, 1464
SinI GGWCC 6 cut(s) 1042, 2181, 2366, 2753, 3016, 3058
SmaI CCCGGG 1 cut(s) 3248
SmiMI CAYNNNNRTG 3 cut(s) 287, 487, 821
SmlI CTYRAG 2 cut(s) 50, 593
SmoI CTYRAG 2 cut(s) 50, 593
SphI GCATGC 1 cut(s) 292
SsiI CCGC 8 cut(s) 625, 1248, 1343, 1555, 2561, 2834, 3002, 3209
SspI AATATT 2 cut(s) 1318, 2413
SspMI CTAG 4 cut(s) 215, 1038, 1397, 2460
SstI GAGCTC 1 cut(s) 1074
StyD4I CCNGG 9 cut(s) 32, 130, 801, 1479, 1769, 2190, 3217, 3245, 3246
StyI CCWWGG 1 cut(s) 699
TaaI ACNGT 3 cut(s) 336, 1156, 1494
TaiI ACGT 2 cut(s) 1117, 2542
TaqI TCGA 7 cut(s) 70, 1464, 2037, 2445, 2771, 2820, 3120
TaqII GACCGA 1 cut(s) 2883
TatI WGTACW 1 cut(s) 2469
TscAI CASTG 6 cut(s) 289, 588, 903, 1195, 1441, 2108
TseFI GTSAC 3 cut(s) 1107, 1183, 1563
Tsp45I GTSAC 3 cut(s) 1107, 1183, 1563
TspGWI ACGGA 2 cut(s) 153, 713
TspMI CCCGGG 1 cut(s) 3246
TspRI CASTG 6 cut(s) 289, 588, 903, 1195, 1441, 2108
Tth111I GACNNNGTC 1 cut(s) 2390
Van91I CCANNNNNTGG 2 cut(s) 749, 3218
VpaK11BI GGWCC 6 cut(s) 1042, 2181, 2366, 2753, 3016, 3058
VspI ATTAAT 1 cut(s) 3194
XagI CCTNNNNNAGG 1 cut(s) 670
XapI RAATTY 7 cut(s) 536, 1325, 1460, 1669, 1994, 2204, 2266
XceI RCATGY 3 cut(s) 197, 292, 1864
XmaI CCCGGG 1 cut(s) 3246
XmiI GTMKAC 1 cut(s) 2165
XmnI GAANNNNTTC 3 cut(s) 792, 878, 1430
XspI CTAG 4 cut(s) 215, 1038, 1397, 2460
Zsp2I ATGCAT 2 cut(s) 199, 934
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.