Belongs to the class-I aminoacyl-tRNA synthetase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 2 cut(s) | 89, 124 |
| AccII | CGCG | 1 cut(s) | 179 |
| AciI | CCGC | 1 cut(s) | 156 |
| AclWI | GGATC | 1 cut(s) | 158 |
| AcsI | RAATTY | 1 cut(s) | 187 |
| AcyI | GRCGYC | 3 cut(s) | 63, 86, 121 |
| AfaI | GTAC | 1 cut(s) | 112 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 107 |
| Alw26I | GTCTC | 2 cut(s) | 243, 290 |
| AlwI | GGATC | 1 cut(s) | 158 |
| ApoI | RAATTY | 1 cut(s) | 187 |
| AspS9I | GGNCC | 1 cut(s) | 98 |
| AsuC2I | CCSGG | 1 cut(s) | 321 |
| AsuHPI | GGTGA | 4 cut(s) | 89, 121, 155, 335 |
| AsuII | TTCGAA | 1 cut(s) | 259 |
| AvaII | GGWCC | 1 cut(s) | 98 |
| BbsI | GAAGAC | 2 cut(s) | 248, 279 |
| BccI | CCATC | 2 cut(s) | 39, 188 |
| BcnI | CCSGG | 1 cut(s) | 321 |
| BcoDI | GTCTC | 2 cut(s) | 243, 290 |
| BglII | AGATCT | 1 cut(s) | 21 |
| BisI | GCNGC | 1 cut(s) | 157 |
| BlsI | GCNGC | 1 cut(s) | 158 |
| Bme1390I | CCNGG | 1 cut(s) | 321 |
| Bme18I | GGWCC | 1 cut(s) | 98 |
| BmgT120I | GGNCC | 1 cut(s) | 98 |
| BmrFI | CCNGG | 1 cut(s) | 321 |
| BpiI | GAAGAC | 2 cut(s) | 248, 279 |
| Bpu10I | CCTNAGC | 1 cut(s) | 219 |
| Bpu14I | TTCGAA | 1 cut(s) | 259 |
| BpuMI | CCSGG | 1 cut(s) | 321 |
| BsaHI | GRCGYC | 3 cut(s) | 63, 86, 121 |
| BsaI | GGTCTC | 1 cut(s) | 243 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 238, 268 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 107 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 107 |
| BseRI | GAGGAG | 1 cut(s) | 308 |
| Bsh1236I | CGCG | 1 cut(s) | 179 |
| Bsh1285I | CGRYCG | 1 cut(s) | 171 |
| BsiEI | CGRYCG | 1 cut(s) | 171 |
| BsiSI | CCGG | 2 cut(s) | 203, 321 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 107 |
| BsmAI | GTCTC | 2 cut(s) | 243, 290 |
| BsmBI | CGTCTC | 1 cut(s) | 290 |
| Bso31I | GGTCTC | 1 cut(s) | 243 |
| Bsp119I | TTCGAA | 1 cut(s) | 259 |
| Bsp143I | GATC | 3 cut(s) | 21, 163, 168 |
| Bsp68I | TCGCGA | 1 cut(s) | 179 |
| BspACI | CCGC | 1 cut(s) | 156 |
| BspFNI | CGCG | 1 cut(s) | 179 |
| BspPI | GGATC | 1 cut(s) | 158 |
| BspT104I | TTCGAA | 1 cut(s) | 259 |
| BspTNI | GGTCTC | 1 cut(s) | 243 |
| BssMI | GATC | 3 cut(s) | 21, 163, 168 |
| BssNI | GRCGYC | 3 cut(s) | 63, 86, 121 |
| Bst4CI | ACNGT | 2 cut(s) | 254, 288 |
| Bst6I | CTCTTC | 1 cut(s) | 98 |
| BstACI | GRCGYC | 3 cut(s) | 63, 86, 121 |
| BstBI | TTCGAA | 1 cut(s) | 259 |
| BstC8I | GCNNGC | 1 cut(s) | 200 |
| BstDEI | CTNAG | 1 cut(s) | 219 |
| BstFNI | CGCG | 1 cut(s) | 179 |
| BstKTI | GATC | 3 cut(s) | 24, 166, 171 |
| BstMAI | GTCTC | 2 cut(s) | 243, 290 |
| BstMBI | GATC | 3 cut(s) | 21, 163, 168 |
| BstMCI | CGRYCG | 1 cut(s) | 171 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 156 |
| BstSCI | CCNGG | 1 cut(s) | 319 |
| BstUI | CGCG | 1 cut(s) | 179 |
| BstV2I | GAAGAC | 2 cut(s) | 248, 279 |
| BstX2I | RGATCY | 2 cut(s) | 21, 163 |
| BstYI | RGATCY | 2 cut(s) | 21, 163 |
| BtuMI | TCGCGA | 1 cut(s) | 179 |
| Cac8I | GCNNGC | 1 cut(s) | 200 |
| Cfr13I | GGNCC | 1 cut(s) | 98 |
| CseI | GACGC | 4 cut(s) | 52, 139, 284, 307 |
| Csp6I | GTAC | 1 cut(s) | 111 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 269, 304 |
| CviJI | RGCY | 4 cut(s) | 49, 174, 198, 314 |
| CviKI_1 | RGCY | 4 cut(s) | 49, 174, 198, 314 |
| CviQI | GTAC | 1 cut(s) | 111 |
| DdeI | CTNAG | 1 cut(s) | 219 |
| DpnI | GATC | 3 cut(s) | 23, 165, 170 |
| DpnII | GATC | 3 cut(s) | 21, 163, 168 |
| Eam1104I | CTCTTC | 1 cut(s) | 98 |
| EarI | CTCTTC | 1 cut(s) | 98 |
| Eco31I | GGTCTC | 1 cut(s) | 243 |
| Eco47I | GGWCC | 1 cut(s) | 98 |
| EcoRI | GAATTC | 1 cut(s) | 187 |
| Esp3I | CGTCTC | 1 cut(s) | 290 |
| FaiI | YATR | 3 cut(s) | 96, 134, 141 |
| Fnu4HI | GCNGC | 1 cut(s) | 157 |
| Fsp4HI | GCNGC | 1 cut(s) | 157 |
| GluI | GCNGC | 1 cut(s) | 157 |
| HapII | CCGG | 2 cut(s) | 203, 321 |
| HgaI | GACGC | 4 cut(s) | 52, 139, 284, 307 |
| Hin1I | GRCGYC | 3 cut(s) | 63, 86, 121 |
| HinfI | GANTC | 2 cut(s) | 7, 206 |
| HpaII | CCGG | 2 cut(s) | 203, 321 |
| HphI | GGTGA | 4 cut(s) | 89, 121, 155, 335 |
| Hpy188I | TCNGA | 3 cut(s) | 21, 168, 193 |
| Hpy188III | TCNNGA | 1 cut(s) | 178 |
| Hpy99I | CGWCG | 5 cut(s) | 45, 88, 123, 126, 155 |
| HpyAV | CCTTC | 1 cut(s) | 47 |
| HpyCH4III | ACNGT | 2 cut(s) | 254, 288 |
| HpyCH4IV | ACGT | 2 cut(s) | 86, 121 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 156 |
| HpyF3I | CTNAG | 1 cut(s) | 219 |
| HpySE526I | ACGT | 2 cut(s) | 86, 121 |
| Hsp92I | GRCGYC | 3 cut(s) | 63, 86, 121 |
| Kzo9I | GATC | 3 cut(s) | 21, 163, 168 |
| LpnPI | CCDG | 4 cut(s) | 197, 216, 295, 334 |
| MaeII | ACGT | 2 cut(s) | 86, 121 |
| MaeIII | GTNAC | 1 cut(s) | 87 |
| MalI | GATC | 3 cut(s) | 23, 165, 170 |
| MboI | GATC | 3 cut(s) | 21, 163, 168 |
| MboII | GAAGA | 5 cut(s) | 115, 173, 241, 248, 284 |
| MflI | RGATCY | 2 cut(s) | 21, 163 |
| MluCI | AATT | 1 cut(s) | 187 |
| MnlI | CCTC | 3 cut(s) | 262, 286, 342 |
| MseI | TTAA | 2 cut(s) | 69, 239 |
| MspI | CCGG | 2 cut(s) | 203, 321 |
| MspR9I | CCNGG | 1 cut(s) | 321 |
| MvnI | CGCG | 1 cut(s) | 179 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 156 |
| NciI | CCSGG | 1 cut(s) | 321 |
| NdeII | GATC | 3 cut(s) | 21, 163, 168 |
| NmuCI | GTSAC | 1 cut(s) | 87 |
| NruI | TCGCGA | 1 cut(s) | 179 |
| NspV | TTCGAA | 1 cut(s) | 259 |
| PfeI | GAWTC | 1 cut(s) | 206 |
| PkrI | GCNGC | 1 cut(s) | 158 |
| Ple19I | CGATCG | 1 cut(s) | 171 |
| PspPI | GGNCC | 1 cut(s) | 98 |
| PsuI | RGATCY | 2 cut(s) | 21, 163 |
| PvuI | CGATCG | 1 cut(s) | 171 |
| RruI | TCGCGA | 1 cut(s) | 179 |
| RsaI | GTAC | 1 cut(s) | 112 |
| RsaNI | GTAC | 1 cut(s) | 111 |
| SaqAI | TTAA | 2 cut(s) | 69, 239 |
| SatI | GCNGC | 1 cut(s) | 157 |
| Sau3AI | GATC | 3 cut(s) | 21, 163, 168 |
| Sau96I | GGNCC | 1 cut(s) | 98 |
| ScrFI | CCNGG | 1 cut(s) | 321 |
| SetI | ASST | 2 cut(s) | 89, 124 |
| SfuI | TTCGAA | 1 cut(s) | 259 |
| SinI | GGWCC | 1 cut(s) | 98 |
| Sse9I | AATT | 1 cut(s) | 187 |
| SsiI | CCGC | 1 cut(s) | 156 |
| SspI | AATATT | 1 cut(s) | 214 |
| StyD4I | CCNGG | 1 cut(s) | 319 |
| TaaI | ACNGT | 2 cut(s) | 254, 288 |
| TaiI | ACGT | 2 cut(s) | 89, 124 |
| TaqI | TCGA | 2 cut(s) | 40, 259 |
| TaqII | GACCGA | 1 cut(s) | 115 |
| TasI | AATT | 1 cut(s) | 187 |
| TauI | GCSGC | 1 cut(s) | 159 |
| TfiI | GAWTC | 1 cut(s) | 206 |
| Tru1I | TTAA | 2 cut(s) | 69, 239 |
| Tru9I | TTAA | 2 cut(s) | 69, 239 |
| TseFI | GTSAC | 1 cut(s) | 87 |
| Tsp45I | GTSAC | 1 cut(s) | 87 |
| TspDTI | ATGAA | 2 cut(s) | 242, 248 |
| VpaK11BI | GGWCC | 1 cut(s) | 98 |
| XapI | RAATTY | 1 cut(s) | 187 |
| ZraI | GACGTC | 2 cut(s) | 87, 122 |