Rh4DG406100

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000598
Physical Location & Seq
Reverse (-)
62005477 .. 62009442
3966 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG406100.1

Sequence Viewer

Length: 2214 bp
ATGGACCGATCCATTCCTGCTTTAAGAAAACTGGTATCCAAAGGTGATAACATTCTCCTAGAGATTAAGATTCTCCTAGAGATTGAAGCAGAGGTTCGAGAATGGTGGGAAGAGAAAGGCGTTTTTGTCAATGCTGATCAATCTCCTGACAACCCTCACTATCCTAACGATGAACTGGGGGATGATCGGAAGAAGTGGTTTGAAAACTATCTGTTTGGCGGTTTATCGCGACGGGCATTCCTGATATCTAAGCTAGAGTGTTGTGCAGCTTATCATAGATTAAGAGATGACAATGTGCCATGGCAAATCAAAGCCTCAGGCGCAGACGAACTTCCGGAGAAGATTATAAATCTGTTGGTTGGAGAACCACCAGTATTTCCAACAGTTTGGAGAGCTGCCTATTCTACTGATGCCATGAGACTCTCCTTAGCTTGTTCTTGTGATGGTGATGATTCTCCGAGAATTTTATTTAATACTGCAAATTCTGCTATCCGAAAGCTCACTAAAGAAATGTCATGGATTAGTGAACAACTAGCTTATATGGCTGCTGCTGCAGACTCTTCTTCTTTTGTGAGAAGAATCAGAAGAGATGGCCCCCCCAGTACTTTTGCGGACAAGGTGTTTGCGAATGAGATAAATATTGCTGTGCATAACAGTGATAACTATTACCGGGCTTGCATGTTTCAAGAAGCCCTCATTTCTGGCTTTCTTCAACTTCAGGCTGCCAGGGACTCGTATAGAATTTCATGTGGTCCTCATGGCATGAACCATGATTTGGTGCTGCGCTATATTGATGTGCATACACGTCTAATTGCTCCAATCTGTCCACACTATGCTGAATATGTCTGGCGGGAATATTTAAAAAAGGAGGGGTTTGTGGTAATTAAGTCAGGCTGGCCTGCCGCTGATGCCCCGGATTTGATACTCCAAAGTGCCAATAAGTATTTGCAAGATCTAATTGAACTTATGAGGGAGCTTGAAAAGAAGTTTCGAGCTTTAAATGATGGTAATTATGATGTAACAGAAGAAGAAACGAAGGCAGTAGGTTTGATATATGTTAAGGAGGAAATTGATGGATGGGAGGGTAAGTGCTTGAGGATACTTCAAGATAACTTTGACAGCGAGACAAACACCTTTTCTGCTCAGGACGAGGACATGCTAGATGCATTAATATCGTACATATATGGGGATTTTACCATTCGTCAGGATATAGATTATAGGCAAACTGAACAGCTCTGTATGTCTTTTCTGAAGTTGAAGAAGGATGAAGCACTGAATTTTGGGGCTGATGCCTTAGAGTTGAAGCTACCATTTGGAGAGATTGAGGTCGTTCAAGAAAACTTGGACTTGATTAAGAGAGAAATTGGCCTTCATGAGGTTAAGGTACAAGTTTTCTCTGGTACCACCGGTCTTGAAGATTTTACACTGGATAGTCCTCCATCCCCTGGAAGGCCAGCTGCTGCCTTGGTGAGCCGTGACTTCTATGAGAAAATGGAACGTTCTGCTCCCGGAGTATGGGAATTGAAACGTCGTCTTCAGTTATCAGATAAAGTTCTTACTCCCTCGTCTCGTCCTCCCTCTGAGCTATTGTACCAGAAGAAAATGGAACATCCGTTTAAGTTGTTATTCCAGGAGAAAACGGAACATTCTGACCTTGGAGAACAGGAACCGAATGATGAACAATCCCCAGATAATATTCTTGCTCCATTATATCATCCTCCCTATGAGCTATTGTTTCAGGGCTCTTTCAAAAAGGCCAAACGTACTGCTTCTGCCGAGGACAAATGGCTCCTCGTGAACTTGCAATCGACTAAGGAATTCAGCTCACATCTGATTAATCAAGACACCTGGGCTAATGAAGCTGTCTCGAAAATCATCAGCACTAATTTTATCTTCTGGCAGGCATATGATGATTCAAATGAAGTCAAGAAGATTTGCAGATTCTACAACTTGGTATATACACCTGTAGTGCTTATCATTGACCCCATAAATGGTGAGAATATGCGTTCGTGGTATGGAATGGTTCAACCAGAACGTCTGCTGGAGGATCTGTTAGTACCATTTTTGGAAAATAGCCCCAAGGATCACCATCATCCAGGAGAAGAAAGTTCTCAGCCTCAGCCAGAGGAAAGTGAAGATGATTCGGTTGGTGACAAAACTGATGAAGAAGATGAGGAATTGCAGTGGGCATTGGAAGCTTCCATGAAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

737

Amino Acids

84.52

Weight (kDa)

4.82

Isoelectric Point (pI)

59.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anticodon_1 PF08264 205 - 330 8.2e-11 Anticodon-binding domain of tRNA ligase
Thioredoxin_7 PF13899 582 - 662 1.3e-12 Thioredoxin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000598)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09620
fragaria_vesca FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_3g34581 FvH4_4g33720 FvH4_4g33720
malus_domestica MD08G1244600.v1.1 MD15G1439400.v1.1
prunus_persica Prupe.1G582000_v2.0.a1 Prupe.2G015100_v2.0.a1 Prupe.2G123100_v2.0.a1
pyrus_communis pycom08g21210 pycom08g21220 pycom15g38730
rosa_chinensis RchiOBHm_Chr1g0336121 RchiOBHm_Chr1g0353811 RchiOBHm_Chr1g0373331 RchiOBHm_Chr4g0443191 RchiOBHm_Chr4g0443201 RchiOBHm_Chr4g0443211
rosa_laevigata RLG00000003122 RLG00000005948 RLG00000026826 RLG00000028251 RLG00000028252 RLG00000028253 RLG00000029377
rosa_multiflora Rmu_co8002498.1_g000001 Rmu_co8262279.1_g000001 Rmu_co8314139.1_g000001 Rmu_co8393711.1_g000001 Rmu_co8419167.1_g000001 Rmu_sc0007968.1_g000004 Rmu_sc0018919.1_g000004 Rmu_sc0027447.1_g000001
rosa_roxburghii Rroxscaffold_4G00284260 Rroxscaffold_4G00301490 Rroxscaffold_4G00315860 Rroxscaffold_5G00383790 Rroxscaffold_5G00383800
rosa_rugosa Rorug01G0123100 Rorug01G0238700 Rorug01G0377100 Rorug01G0377100 Rorug01G0377200 Rorug01G0377300 Rorug04G0344700 Rorug05G0347900.1
rosa_samantha Rh1AG143800 Rh1AG250300 Rh1AG386300 Rh1BG113400 Rh1BG220800 Rh1BG220900 Rh1BG350400 Rh1CG135500 Rh1CG233100 Rh1CG363500 Rh1DG150200 Rh1DG247700 Rh1DG247800 Rh1DG381300 Rh4AG399400 Rh4AG399500 Rh4BG411000 Rh4BG411100 Rh4BG411200 Rh4CG426500 Rh4CG426600 Rh4DG406100 Rh4DG406200 Rh4DG406300
rosa_wichuraiana Rw0G015190 Rw1G021850 Rw1G034870 Rw4G034440 Rw4G034450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 347
Acc65I GGTACC 1 cut(s) 1400
AccB1I GGYRCC 1 cut(s) 1400
AccB7I CCANNNNNTGG 2 cut(s) 775, 1445
AccII CGCG 1 cut(s) 229
AccIII TCCGGA 1 cut(s) 334
AciI CCGC 4 cut(s) 219, 611, 850, 903
AclI AACGTT 1 cut(s) 1498
AclWI GGATC 3 cut(s) 3, 2055, 2091
AcsI RAATTY 5 cut(s) 462, 481, 741, 1276, 1817
AcuI CTGAAG 3 cut(s) 701, 1271, 1520
AfaI GTAC 7 cut(s) 604, 1178, 1386, 1402, 1592, 1765, 2058
AfiI CCNNNNNNNGG 6 cut(s) 775, 1375, 1445, 1449, 1515, 1991
AflIII ACRYGT 1 cut(s) 803
AgeI ACCGGT 1 cut(s) 1406
AjiI CACGTC 1 cut(s) 806
AjnI CCWGG 5 cut(s) 725, 1444, 1629, 1847, 2095
AjuI GAANNNNNNNTTGG 2 cut(s) 758, 790
Alw26I GTCTC 4 cut(s) 412, 1118, 1572, 1870
AlwI GGATC 3 cut(s) 3, 2055, 2091
AlwNI CAGNNNCTG 1 cut(s) 1460
Aor13HI TCCGGA 1 cut(s) 334
AoxI GGCC 5 cut(s) 592, 896, 1366, 1451, 1755
ApeKI GCWGC 9 cut(s) 266, 395, 545, 548, 551, 722, 781, 1457, 1460
ApoI RAATTY 5 cut(s) 462, 481, 741, 1276, 1817
ArsI GACNNNNNNTTYG 2 cut(s) 605, 637
AseI ATTAAT 2 cut(s) 1169, 1836
AsiGI ACCGGT 1 cut(s) 1406
Asp718I GGTACC 1 cut(s) 1400
AspLEI GCGC 2 cut(s) 323, 786
AspS9I GGNCC 3 cut(s) 4, 593, 752
AsuC2I CCSGG 3 cut(s) 671, 914, 1509
AsuHPI GGTGA 6 cut(s) 56, 458, 1480, 2006, 2078, 2162
AvaII GGWCC 2 cut(s) 4, 752
AxyI CCTNAGG 1 cut(s) 316
BanI GGYRCC 1 cut(s) 1400
BanII GRGCYC 1 cut(s) 1745
BauI CACGAG 1 cut(s) 1793
BbsI GAAGAC 1 cut(s) 1526
BbvCI CCTCAGC 1 cut(s) 2118
BbvI GCAGC 9 cut(s) 278, 382, 532, 535, 538, 709, 768, 1444, 1447
BccI CCATC 7 cut(s) 437, 584, 998, 1067, 1071, 1447, 2097
BceAI ACGGC 1 cut(s) 1458
BcgI CGANNNNNNTGC 2 cut(s) 1155, 1189
BciT130I CCWGG 5 cut(s) 727, 1446, 1631, 1849, 2097
BciVI GTATCC 2 cut(s) 46, 1092
BclI TGATCA 1 cut(s) 136
BcnI CCSGG 3 cut(s) 671, 914, 1509
BcoDI GTCTC 4 cut(s) 412, 1118, 1572, 1870
BfaI CTAG 5 cut(s) 59, 77, 254, 533, 1160
BfmI CTRYAG 2 cut(s) 552, 1965
BfuI GTATCC 2 cut(s) 46, 1092
BglII AGATCT 1 cut(s) 952
BmcAI AGTACT 1 cut(s) 604
Bme1390I CCNGG 8 cut(s) 671, 727, 914, 1446, 1509, 1631, 1849, 2097
Bme18I GGWCC 2 cut(s) 4, 752
BmgBI CACGTC 1 cut(s) 806
BmgT120I GGNCC 3 cut(s) 4, 593, 752
BmiI GGNNCC 4 cut(s) 595, 1402, 1668, 1790
BmrFI CCNGG 8 cut(s) 671, 727, 914, 1446, 1509, 1631, 1849, 2097
BmrI ACTGGG 2 cut(s) 185, 594
BmsI GCATC 4 cut(s) 400, 898, 1153, 1279
BmuI ACTGGG 2 cut(s) 185, 594
BpiI GAAGAC 1 cut(s) 1526
BpmI CTGGAG 1 cut(s) 2063
Bpu10I CCTNAGC 3 cut(s) 427, 1143, 2118
BpuEI CTTGAG 1 cut(s) 1114
BpuMI CCSGG 3 cut(s) 671, 914, 1509
BsaBI GATNNNNATC 1 cut(s) 2088
BsaJI CCNNGG 9 cut(s) 299, 726, 912, 1444, 1464, 1654, 1776, 1848, 2079
BsaWI WCCGGW 2 cut(s) 334, 1406
BsaXI ACNNNNNCTCC 4 cut(s) 1073, 1103, 2036, 2066
Bsc4I CCNNNNNNNGG 6 cut(s) 775, 1375, 1445, 1449, 1515, 1991
Bse118I RCCGGY 1 cut(s) 1406
Bse1I ACTGG 5 cut(s) 36, 180, 371, 600, 1431
Bse21I CCTNAGG 1 cut(s) 316
Bse8I GATNNNNATC 1 cut(s) 2088
BseAI TCCGGA 1 cut(s) 334
BseBI CCWGG 5 cut(s) 727, 1446, 1631, 1849, 2097
BseDI CCNNGG 9 cut(s) 299, 726, 912, 1444, 1464, 1654, 1776, 1848, 2079
BseGI GGATG 7 cut(s) 187, 1082, 1270, 1439, 1609, 1714, 2092
BseJI GATNNNNATC 1 cut(s) 2088
BseLI CCNNNNNNNGG 6 cut(s) 775, 1375, 1445, 1449, 1515, 1991
BseMII CTCAG 5 cut(s) 330, 1157, 1572, 2126, 2132
BseNI ACTGG 5 cut(s) 36, 180, 371, 600, 1431
BseRI GAGGAG 1 cut(s) 1781
BseXI GCAGC 9 cut(s) 278, 382, 532, 535, 538, 709, 768, 1444, 1447
BsgI GTGCAG 1 cut(s) 285
Bsh1236I CGCG 1 cut(s) 229
BshFI GGCC 5 cut(s) 594, 898, 1368, 1453, 1757
BshNI GGYRCC 1 cut(s) 1400
BshTI ACCGGT 1 cut(s) 1406
BsiSI CCGG 5 cut(s) 335, 670, 914, 1407, 1509
BslFI GGGAC 1 cut(s) 743
BslI CCNNNNNNNGG 6 cut(s) 775, 1375, 1445, 1449, 1515, 1991
BsmAI GTCTC 4 cut(s) 412, 1118, 1572, 1870
BsmBI CGTCTC 1 cut(s) 1572
BsmFI GGGAC 1 cut(s) 743
BsmI GAATGC 1 cut(s) 236
BsnI GGCC 5 cut(s) 594, 898, 1368, 1453, 1757
Bsp1286I GDGCHC 1 cut(s) 1745
Bsp13I TCCGGA 1 cut(s) 334
Bsp143I GATC 6 cut(s) 8, 136, 184, 952, 2047, 2083
Bsp19I CCATGG 1 cut(s) 299
Bsp68I TCGCGA 1 cut(s) 229
BspACI CCGC 4 cut(s) 219, 611, 850, 903
BspANI GGCC 5 cut(s) 594, 898, 1368, 1453, 1757
BspCNI CTCAG 5 cut(s) 329, 1156, 1573, 2125, 2131
BspEI TCCGGA 1 cut(s) 334
BspFNI CGCG 1 cut(s) 229
BspHI TCATGA 1 cut(s) 1372
BspLI GGNNCC 4 cut(s) 595, 1402, 1668, 1790
BspMAI CTGCAG 1 cut(s) 556
BspPI GGATC 3 cut(s) 3, 2055, 2091
BspT107I GGYRCC 1 cut(s) 1400
BsrFI RCCGGY 1 cut(s) 1406
BsrI ACTGG 5 cut(s) 36, 180, 371, 600, 1431
BssAI RCCGGY 1 cut(s) 1406
BssECI CCNNGG 9 cut(s) 299, 726, 912, 1444, 1464, 1654, 1776, 1848, 2079
BssMI GATC 6 cut(s) 8, 136, 184, 952, 2047, 2083
BssSI CACGAG 1 cut(s) 1793
BssT1I CCWWGG 4 cut(s) 299, 1464, 1654, 2079
Bst2BI CACGAG 1 cut(s) 1793
Bst2UI CCWGG 5 cut(s) 727, 1446, 1631, 1849, 2097
Bst4CI ACNGT 2 cut(s) 385, 656
Bst6I CTCTTC 3 cut(s) 105, 565, 580
BstAPI GCANNNNNTGC 1 cut(s) 485
BstC8I GCNNGC 5 cut(s) 676, 896, 900, 1455, 1902
BstDEI CTNAG 9 cut(s) 249, 316, 427, 1143, 1294, 1581, 1812, 2112, 2118
BstDSI CCRYGG 1 cut(s) 299
BstENI CCTNNNNNAGG 1 cut(s) 1373
BstF5I GGATG 7 cut(s) 187, 1082, 1270, 1439, 1609, 1714, 2092
BstFNI CGCG 1 cut(s) 229
BstHHI GCGC 2 cut(s) 323, 786
BstKTI GATC 6 cut(s) 11, 139, 187, 955, 2050, 2086
BstMAI GTCTC 4 cut(s) 412, 1118, 1572, 1870
BstMBI GATC 6 cut(s) 8, 136, 184, 952, 2047, 2083
BstMWI GCNNNNNNNGC 7 cut(s) 320, 485, 542, 551, 908, 1859, 2195
BstNI CCWGG 5 cut(s) 727, 1446, 1631, 1849, 2097
BstNSI RCATGY 2 cut(s) 682, 1159
BstSCI CCNGG 8 cut(s) 669, 725, 912, 1444, 1507, 1629, 1847, 2095
BstSFI CTRYAG 2 cut(s) 552, 1965
BstUI CGCG 1 cut(s) 229
BstV1I GCAGC 9 cut(s) 278, 382, 532, 535, 538, 709, 768, 1444, 1447
BstV2I GAAGAC 1 cut(s) 1526
BstX2I RGATCY 2 cut(s) 952, 2047
BstXI CCANNNNNNTGG 1 cut(s) 387
BstYI RGATCY 2 cut(s) 952, 2047
Bsu36I CCTNAGG 1 cut(s) 316
BsuI GTATCC 2 cut(s) 46, 1092
BsuRI GGCC 5 cut(s) 594, 898, 1368, 1453, 1757
BtgI CCRYGG 1 cut(s) 299
BtrI CACGTC 1 cut(s) 806
BtsCI GGATG 7 cut(s) 187, 1082, 1270, 1439, 1609, 1714, 2092
BtsI GCAGTG 1 cut(s) 2189
BtsIMutI CAGTG 4 cut(s) 661, 1271, 1424, 2189
BtuMI TCGCGA 1 cut(s) 229
Cac8I GCNNGC 5 cut(s) 676, 896, 900, 1455, 1902
CaiI CAGNNNCTG 1 cut(s) 1460
CciI TCATGA 1 cut(s) 1372
CfoI GCGC 2 cut(s) 323, 786
Cfr10I RCCGGY 1 cut(s) 1406
Cfr13I GGNCC 3 cut(s) 4, 593, 752
Csp6I GTAC 7 cut(s) 603, 1177, 1385, 1401, 1591, 1764, 2057
CspAI ACCGGT 1 cut(s) 1406
CspCI CAANNNNNGTGG 2 cut(s) 1393, 1428
CviQI GTAC 7 cut(s) 603, 1177, 1385, 1401, 1591, 1764, 2057
DdeI CTNAG 9 cut(s) 249, 316, 427, 1143, 1294, 1581, 1812, 2112, 2118
DpnI GATC 6 cut(s) 10, 138, 186, 954, 2049, 2085
DpnII GATC 6 cut(s) 8, 136, 184, 952, 2047, 2083
DraI TTTAAA 2 cut(s) 861, 999
Eam1104I CTCTTC 3 cut(s) 105, 565, 580
EarI CTCTTC 3 cut(s) 105, 565, 580
Eco130I CCWWGG 4 cut(s) 299, 1464, 1654, 2079
Eco24I GRGCYC 1 cut(s) 1745
Eco32I GATATC 1 cut(s) 246
Eco47I GGWCC 2 cut(s) 4, 752
Eco57I CTGAAG 3 cut(s) 701, 1271, 1520
Eco81I CCTNAGG 1 cut(s) 316
EcoNI CCTNNNNNAGG 1 cut(s) 1373
EcoRI GAATTC 1 cut(s) 1817
EcoRII CCWGG 5 cut(s) 725, 1444, 1629, 1847, 2095
EcoRV GATATC 1 cut(s) 246
EcoT14I CCWWGG 4 cut(s) 299, 1464, 1654, 2079
EcoT22I ATGCAT 1 cut(s) 1168
EcoT38I GRGCYC 1 cut(s) 1745
ErhI CCWWGG 4 cut(s) 299, 1464, 1654, 2079
Esp3I CGTCTC 1 cut(s) 1572
FaqI GGGAC 1 cut(s) 743
FauI CCCGC 1 cut(s) 843
FauNDI CATATG 1 cut(s) 1906
FbaI TGATCA 1 cut(s) 136
FokI GGATG 7 cut(s) 194, 1089, 1277, 1426, 1596, 1701, 2079
FriOI GRGCYC 1 cut(s) 1745
FspBI CTAG 5 cut(s) 59, 77, 254, 533, 1160
GlaI GCGC 2 cut(s) 322, 785
GsuI CTGGAG 1 cut(s) 2063
HaeIII GGCC 5 cut(s) 594, 898, 1368, 1453, 1757
HapII CCGG 5 cut(s) 335, 670, 914, 1407, 1509
HhaI GCGC 2 cut(s) 323, 786
Hin6I GCGC 2 cut(s) 321, 784
HinP1I GCGC 2 cut(s) 321, 784
HindIII AAGCTT 1 cut(s) 2196
HinfI GANTC 9 cut(s) 70, 420, 452, 557, 579, 731, 1913, 1941, 2141
HpaII CCGG 5 cut(s) 335, 670, 914, 1407, 1509
HphI GGTGA 6 cut(s) 56, 458, 1480, 2006, 2078, 2162
Hpy166II GTNNAC 3 cut(s) 527, 827, 1798
Hpy188I TCNGA 9 cut(s) 189, 459, 494, 584, 1251, 1546, 1582, 1651, 1833
Hpy8I GTNNAC 3 cut(s) 527, 827, 1798
Hpy99I CGWCG 2 cut(s) 234, 1533
HpyAV CCTTC 4 cut(s) 1030, 1255, 1379, 1443
HpyCH4III ACNGT 2 cut(s) 385, 656
HpyCH4IV ACGT 5 cut(s) 805, 1498, 1528, 1762, 2035
HpyF10VI GCNNNNNNNGC 7 cut(s) 320, 485, 542, 551, 908, 1859, 2195
HpyF3I CTNAG 9 cut(s) 249, 316, 427, 1143, 1294, 1581, 1812, 2112, 2118
HpySE526I ACGT 5 cut(s) 805, 1498, 1528, 1762, 2035
HspAI GCGC 2 cut(s) 321, 784
Kpn2I TCCGGA 1 cut(s) 334
KpnI GGTACC 1 cut(s) 1404
Ksp22I TGATCA 1 cut(s) 136
Kzo9I GATC 6 cut(s) 8, 136, 184, 952, 2047, 2083
LmnI GCTCC 5 cut(s) 820, 973, 1510, 1708, 1794
Lsp1109I GCAGC 9 cut(s) 278, 382, 532, 535, 538, 709, 768, 1444, 1447
LweI GCATC 4 cut(s) 400, 898, 1153, 1279
MaeI CTAG 5 cut(s) 59, 77, 254, 533, 1160
MaeII ACGT 5 cut(s) 805, 1498, 1528, 1762, 2035
MaeIII GTNAC 3 cut(s) 1018, 1475, 2150
MalI GATC 6 cut(s) 10, 138, 186, 954, 2049, 2085
MboI GATC 6 cut(s) 8, 136, 184, 952, 2047, 2083
MflI RGATCY 2 cut(s) 952, 2047
MhlI GDGCHC 1 cut(s) 1745
MlyI GAGTC 3 cut(s) 414, 551, 725
MmeI TCCRAC 2 cut(s) 340, 404
Mph1103I ATGCAT 1 cut(s) 1168
MroI TCCGGA 1 cut(s) 334
MslI CAYNNNNRTG 1 cut(s) 654
MspA1I CMGCKG 2 cut(s) 905, 1457
MspI CCGG 5 cut(s) 335, 670, 914, 1407, 1509
MspR9I CCNGG 8 cut(s) 671, 727, 914, 1446, 1509, 1631, 1849, 2097
Mva1269I GAATGC 1 cut(s) 236
MvaI CCWGG 5 cut(s) 727, 1446, 1631, 1849, 2097
MvnI CGCG 1 cut(s) 229
MwoI GCNNNNNNNGC 7 cut(s) 320, 485, 542, 551, 908, 1859, 2195
NciI CCSGG 3 cut(s) 671, 914, 1509
NcoI CCATGG 1 cut(s) 299
NdeI CATATG 1 cut(s) 1906
NdeII GATC 6 cut(s) 8, 136, 184, 952, 2047, 2083
NlaIV GGNNCC 4 cut(s) 595, 1402, 1668, 1790
NmeAIII GCCGAG 1 cut(s) 1801
NmuCI GTSAC 2 cut(s) 1475, 2150
NruI TCGCGA 1 cut(s) 229
NsiI ATGCAT 1 cut(s) 1168
NspI RCATGY 2 cut(s) 682, 1159
PagI TCATGA 1 cut(s) 1372
PctI GAATGC 1 cut(s) 236
PfeI GAWTC 6 cut(s) 70, 452, 579, 1913, 1941, 2141
PflMI CCANNNNNTGG 2 cut(s) 775, 1445
PfoI TCCNGGA 3 cut(s) 1507, 1629, 2095
PinAI ACCGGT 1 cut(s) 1406
PleI GAGTC 3 cut(s) 414, 551, 725
PpsI GAGTC 3 cut(s) 414, 551, 725
PshBI ATTAAT 2 cut(s) 1169, 1836
PsiI TTATAA 1 cut(s) 347
Psp1406I AACGTT 1 cut(s) 1498
Psp6I CCWGG 5 cut(s) 725, 1444, 1629, 1847, 2095
PspGI CCWGG 5 cut(s) 725, 1444, 1629, 1847, 2095
PspN4I GGNNCC 4 cut(s) 595, 1402, 1668, 1790
PspPI GGNCC 3 cut(s) 4, 593, 752
PstI CTGCAG 1 cut(s) 556
PstNI CAGNNNCTG 1 cut(s) 1460
PsuI RGATCY 2 cut(s) 952, 2047
PvuII CAGCTG 1 cut(s) 1457
RruI TCGCGA 1 cut(s) 229
RsaI GTAC 7 cut(s) 604, 1178, 1386, 1402, 1592, 1765, 2058
RsaNI GTAC 7 cut(s) 603, 1177, 1385, 1401, 1591, 1764, 2057
RseI CAYNNNNRTG 1 cut(s) 654
Sau3AI GATC 6 cut(s) 8, 136, 184, 952, 2047, 2083
Sau96I GGNCC 3 cut(s) 4, 593, 752
ScaI AGTACT 1 cut(s) 604
SchI GAGTC 3 cut(s) 414, 551, 725
ScrFI CCNGG 8 cut(s) 671, 727, 914, 1446, 1509, 1631, 1849, 2097
SduI GDGCHC 1 cut(s) 1745
SfaNI GCATC 4 cut(s) 400, 898, 1153, 1279
SfcI CTRYAG 2 cut(s) 552, 1965
SinI GGWCC 2 cut(s) 4, 752
SmiMI CAYNNNNRTG 1 cut(s) 654
SmlI CTYRAG 1 cut(s) 1093
SmoI CTYRAG 1 cut(s) 1093
SsiI CCGC 4 cut(s) 219, 611, 850, 903
SspI AATATT 3 cut(s) 640, 857, 1696
SspMI CTAG 5 cut(s) 59, 77, 254, 533, 1160
StyD4I CCNGG 8 cut(s) 669, 725, 912, 1444, 1507, 1629, 1847, 2095
StyI CCWWGG 4 cut(s) 299, 1464, 1654, 2079
TaaI ACNGT 2 cut(s) 385, 656
TaiI ACGT 5 cut(s) 808, 1501, 1531, 1765, 2038
TaqI TCGA 4 cut(s) 97, 991, 1808, 1868
TaqII GACCGA 1 cut(s) 21
TatI WGTACW 1 cut(s) 602
TauI GCSGC 1 cut(s) 905
TfiI GAWTC 6 cut(s) 70, 452, 579, 1913, 1941, 2141
TscAI CASTG 4 cut(s) 661, 1278, 1431, 2189
TseFI GTSAC 2 cut(s) 1475, 2150
TseI GCWGC 9 cut(s) 266, 395, 545, 548, 551, 722, 781, 1457, 1460
Tsp45I GTSAC 2 cut(s) 1475, 2150
TspDTI ATGAA 9 cut(s) 186, 735, 779, 1281, 1361, 1692, 1872, 1935, 2178
TspGWI ACGGA 2 cut(s) 1602, 1655
TspRI CASTG 4 cut(s) 661, 1278, 1431, 2189
Van91I CCANNNNNTGG 2 cut(s) 775, 1445
VpaK11BI GGWCC 2 cut(s) 4, 752
VspI ATTAAT 2 cut(s) 1169, 1836
XagI CCTNNNNNAGG 1 cut(s) 1373
XapI RAATTY 5 cut(s) 462, 481, 741, 1276, 1817
XceI RCATGY 2 cut(s) 682, 1159
XspI CTAG 5 cut(s) 59, 77, 254, 533, 1160
ZrmI AGTACT 1 cut(s) 604
Zsp2I ATGCAT 1 cut(s) 1168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.