Prupe.2G015100_v2.0.a1

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000598
Physical Location & Seq
Reverse (-)
1343821 .. 1347707
3887 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G015100.1

Sequence Viewer

Length: 3336 bp
ATGGAAGCCGAAGCTGAGAAGAGCTTAGTTAAGAGAAATTTTCTCAGAGACAATGAAGTAAAGATTCAAATGTTGTGGAAAGAGCATGATGTTTACAGGGCTGAATCTTGTGAAAAGCCTCCTGAACCAGGGGAGAAGTTCTTTGGAAACTTCCCATTCCCTTACATGAATGGTTCTTTGCATCATGCACATGCATTCTCACTCTCAAAGCTAGAGTTTTCTGCACGTTATCATAGGTTAAGAGGCGCCGATGTTCTCTTGCCTTTTGCTTTTCACGGCACTGGCATGCCCATCAAAGCCTCAGCTGATAAGCTTGCACGAGAGATCAAACAGTTTGGGAACCCACCAGTGTTTCCCCAAAGTGACAAAGAGGAGCAAGGGAACCAGCAGGAAGCAGAAGATGAAAAAGCAGGTGCACCTCCAGATAATAAGTTAAAGGGAAAGAATGAGATACCTGAATTCCAAGAACCGTCTAAGTGGTTGAACTATTTCCCTCCATTGGCAGTGGAATACCTAAAGGCTTTTGGCTTGGGATGTGACTGGAGCCGCTCCTTTGTTACTACAGACTTGAATCCATATTTTGATAAGTTTGTGAGGTGGCAGATGTGGAAATTGAAAGAAATAGGTAAGATTGTGAAAGATAAACGTTACACAATCTACTCTCCATTGGATGGAAAGCCATGTGCAGATCATGATAGGGCAACTGGTGAAGGAGTTCAGCCCCAAGAATACACAATCATCAAAATGGAGGTAGTGGCACCTTTTCCTGCTAAGTTGGGAGTATTGGAAGGGAGGAAAGTTTTCCTTGCTGCAGCAACTTTGAGACCAGAAACCATGTGTGGACAAACAAATGCATGGGTTTTGCCCAATGGAAAGTATGGAGCCTTTGAAATCAACGACACTGATGTATTTGTTCTTACTCAGAGAGCAGCACTTAACCTCGCCTATCAGGAGTATTCTAGAGTCCCTGAGAAGCCTACTTGCTTGGTTGATCTCACTGGTTATGATTTGATTGGTCTTCCATTGAAATCGCCACTTGCACTCAATCAGATCATTTACGCCCTTCCAATGTTGACAATCCTAACAGACAAAGGTACTGGGATTGTGACCAGTGTCCCTTCTGATGCTCCTGATGATTTTATGGCCTTGCATGATTTAAAATTGAAGCCAGCTTTCAGGTCAAAATATGATAAGGCTGCAGAAAAGGTGTGTGTAGATCTCAAAATCAAAAGCCAGAATGACAGAGAGAAGCTAGCAGAAGCCAAGAGGTTGACCTACTTGAAAGGATTTACAGAAGGAACAATGCTTGTGGGAGAATTCAACGGAAGGAAAGTTCAGGAGGCGAAAGCACTGTTAAGGAGCAAGCTGATAGAGGCAGGTGATGCAATTATGTATAGTGAACCTGAGAAGCGTGTAGTGCCAAGGTCGGGTGATGAATGTCTTGTGGCATGTACCGAGCCATGGTACATCACATATGGCGAAGCAGAATGGAAAAAACAGGCTCAGGAGTACTTATCCAGCATGAACTTTTATTCTGACATGACACGGCATGGATTTGAACATACATTGACTTGGTTGAATCAGTGGGCTTGCTCACGATCATTTGGGCTTGGGACGCGCATTCCCTGGGATGAAAAGTACTTTGTCGAATCGTTGTCTGATTCCACCATTTACATGGCTTATTACACCATTGCTCACTTGTTGCACAATGAAGACATGTATGGGGGTTCCAGCACATCTGGGGTTACACCTGAGCAAATGACCAATGAAGTTTGGGATTTTATTTTCTGTGATGGCCCATTTCCAAAATCATCTGAGGTGTCACAATTAATTCTGAATAAGATGAAGCAGGAGTTTGAGTACTGGTATCCTTTTGATCTTCGAGTGTCTGGTAAGGATCTAATCCAGAATCATTTAACCTTTTGCATTTACAACGACACAGCAATTATGTCCAAGAAGCACTGGCCTCGTGGATTTAGATGCAATGGGCACCTCATGCTTGATTCAATGAAGATGTCCAAGTCCACAGGGAACTTTATGACATTGCGTGAGGCAATTGCAGAATATTCTGCTGATGCCACACGGTTCTCTTTGGCTGATGCTGGTGATGGTGTTGATGATGCAAATTTTGTTTCTAGTACTGCAAATAAGGCCATCCTAGATCTCAGTAAAGAGATCTCATGGATGGAAGAACAATTGGGTGCAGACACGTTGAGAATAGGTCCCCCTTCTACTTTTGCTGACAGGGTCTTTGCGAATGAGATGAATATTGCTGTGAAGATGACTGAGCAGAATTACCAGGCTTGCAAGTTCCGAGAGGCCCTTATTACTGGCTTTGTTGGTCTTCAAGCTGCCAGGAAGTGGCACCGGATTTCATGTGGCTCTCAAGAAATGAACCGTGATTTGGTGTGGCGCTTTATGGATGTACAGACACGTTTAATTGCTCCAATCTGTCCACATTATACAGAATATGTCTGGAGGGAGCTTTTGAAGAAGGATGGGTTTGTGGTAAATGCAGGCTGGCCTGCAGCTGATGCTCCAGACTTAACCCTCCGGAGTGCCAAAAAGTATGTGGAAGACTTGATTGGTTCAATGATGAAGCTGTACAACAAGCAAAAGGCAAATTTGACCAATAAGAAGGTGATAGGCTTGATATGTGTTAAGGAACAATTTGATGAATGGAAGATAGAGTGCTTGAGGATACTCCAGAATAACTTCAACAGAGAGACTCGTTTTGCTGCAGACAGTGTCATATTGGAGGCATTGCAAAGTAGTTCTGTCAATCAGGGTAAAGATTTTAGACAAACCCAAAAGCTTTGTATGCCTTTCTTGAAGTCAAAGAAGAAGGATGCAGTTGAACTTGGGGCTCAGGCCTTAGACTTAAAGCTACCATTTGGAGAGATTGAGGTCCTTCAACAGAACTTGGACTTGGTTAAGAGACAAGTCAAACTTGAAGAGGTGGAAGTTTTGTCTGCTACCAACCCAGATGATCGTGCTAAAGCGGGCTCTCATGTTAAACAGATAGAGCAAAATCCTCCATCCCCTGGAAGTCCAACGACCATCTTCTTGACCTGTTCATTAGCTATAATCTTTTTGGTTGATATCTACAATTTACTTACAGAACTCTCTTTATTCTTGGAATATTCTCCAGAACATCATATTGTTCTTCAAATTGGTGAAAATAATTTGGAAAACCTGGCCAAAGTGACTGAGCTTGCTGAACCTTATAAATATGATAAGATCAATTTTAATTGTGGATGTCCAAGTCCAAGAGTGGCTGGACATGGGTGCTTTGGTCTTTACAAGGAAAGTTTGTTGCTGAGTCTATGTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

1112

Amino Acids

126.13

Weight (kDa)

6.06

Isoelectric Point (pI)

42.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000598)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09620
fragaria_vesca FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_3g34581 FvH4_4g33720 FvH4_4g33720
malus_domestica MD08G1244600.v1.1 MD15G1439400.v1.1
prunus_persica Prupe.1G582000_v2.0.a1 Prupe.2G015100_v2.0.a1 Prupe.2G123100_v2.0.a1
pyrus_communis pycom08g21210 pycom08g21220 pycom15g38730
rosa_chinensis RchiOBHm_Chr1g0336121 RchiOBHm_Chr1g0353811 RchiOBHm_Chr1g0373331 RchiOBHm_Chr4g0443191 RchiOBHm_Chr4g0443201 RchiOBHm_Chr4g0443211
rosa_laevigata RLG00000003122 RLG00000005948 RLG00000026826 RLG00000028251 RLG00000028252 RLG00000028253 RLG00000029377
rosa_multiflora Rmu_co8002498.1_g000001 Rmu_co8262279.1_g000001 Rmu_co8314139.1_g000001 Rmu_co8393711.1_g000001 Rmu_co8419167.1_g000001 Rmu_sc0007968.1_g000004 Rmu_sc0018919.1_g000004 Rmu_sc0027447.1_g000001
rosa_roxburghii Rroxscaffold_4G00284260 Rroxscaffold_4G00301490 Rroxscaffold_4G00315860 Rroxscaffold_5G00383790 Rroxscaffold_5G00383800
rosa_rugosa Rorug01G0123100 Rorug01G0238700 Rorug01G0377100 Rorug01G0377100 Rorug01G0377200 Rorug01G0377300 Rorug04G0344700 Rorug05G0347900.1
rosa_samantha Rh1AG143800 Rh1AG250300 Rh1AG386300 Rh1BG113400 Rh1BG220800 Rh1BG220900 Rh1BG350400 Rh1CG135500 Rh1CG233100 Rh1CG363500 Rh1DG150200 Rh1DG247700 Rh1DG247800 Rh1DG381300 Rh4AG399400 Rh4AG399500 Rh4BG411000 Rh4BG411100 Rh4BG411200 Rh4CG426500 Rh4CG426600 Rh4DG406100 Rh4DG406200 Rh4DG406300
rosa_wichuraiana Rw0G015190 Rw1G021850 Rw1G034870 Rw4G034440 Rw4G034450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3228
AarI CACCTGC 2 cut(s) 401, 1367
Acc36I ACCTGC 2 cut(s) 401, 1367
AccB1I GGYRCC 4 cut(s) 245, 757, 1989, 2364
AccB7I CCANNNNNTGG 3 cut(s) 671, 2361, 3044
AccBSI CCGCTC 1 cut(s) 549
AccII CGCG 1 cut(s) 1618
AccIII TCCGGA 1 cut(s) 2553
AciI CCGC 2 cut(s) 547, 3002
AclI AACGTT 1 cut(s) 646
AclWI GGATC 1 cut(s) 1905
AcoI YGGCCR 1 cut(s) 3198
AcsI RAATTY 5 cut(s) 37, 458, 1316, 2125, 2623
AcyI GRCGYC 1 cut(s) 246
AfaI GTAC 9 cut(s) 1096, 1453, 1466, 1511, 1640, 1862, 2140, 2427, 2606
AfiI CCNNNNNNNGG 8 cut(s) 128, 499, 671, 1427, 1461, 2361, 2404, 3044
AflIII ACRYGT 3 cut(s) 1716, 2208, 2432
AjnI CCWGG 6 cut(s) 127, 1625, 2298, 2354, 3043, 3195
AjuI GAANNNNNNNTTGG 6 cut(s) 2387, 2419, 2568, 2600, 2912, 2944
AloI GAACNNNNNNTCC 2 cut(s) 1317, 1349
Alw21I GWGCWC 1 cut(s) 418
Alw26I GTCTC 4 cut(s) 42, 817, 2720, 2932
Alw44I GTGCAC 1 cut(s) 414
AlwI GGATC 1 cut(s) 1905
Aor13HI TCCGGA 1 cut(s) 2553
AoxI GGCC 8 cut(s) 1143, 1795, 1964, 2151, 2319, 2522, 2871, 3198
ApaLI GTGCAC 1 cut(s) 414
ApeKI GCWGC 7 cut(s) 809, 812, 929, 1196, 2351, 2528, 2738
ApoI RAATTY 5 cut(s) 37, 458, 1316, 2125, 2623
ArsI GACNNNNNNTTYG 2 cut(s) 2234, 2266
AseI ATTAAT 1 cut(s) 1829
Asp700I GAANNNNTTC 4 cut(s) 488, 714, 800, 2714
AspLEI GCGC 3 cut(s) 248, 1620, 2415
AspS9I GGNCC 4 cut(s) 1796, 2222, 2320, 2908
AsuHPI GGTGA 6 cut(s) 719, 1391, 1442, 2117, 2653, 3188
AsuNHI GCTAGC 1 cut(s) 1252
AvaII GGWCC 2 cut(s) 2222, 2908
BaeGI GKGCMC 2 cut(s) 418, 1992
BalI TGGCCA 1 cut(s) 3200
BanI GGYRCC 4 cut(s) 245, 757, 1989, 2364
BanII GRGCYC 2 cut(s) 2869, 3008
BauI CACGAG 2 cut(s) 318, 1968
BbsI GAAGAC 4 cut(s) 1010, 1719, 2336, 2583
Bbv12I GWGCWC 1 cut(s) 418
BbvCI CCTCAGC 1 cut(s) 301
BbvI GCAGC 7 cut(s) 796, 824, 941, 1183, 2338, 2540, 2725
BccI CCATC 9 cut(s) 299, 665, 1787, 2102, 2162, 2179, 2492, 3046, 3068
BceAI ACGGC 2 cut(s) 292, 1562
BcgI CGANNNNNNTGC 2 cut(s) 1949, 1983
BciT130I CCWGG 6 cut(s) 129, 1627, 2300, 2356, 3045, 3197
BciVI GTATCC 2 cut(s) 1878, 2694
BcoDI GTCTC 4 cut(s) 42, 817, 2720, 2932
BfaI CTAG 5 cut(s) 212, 960, 1253, 2136, 2159
BfmI CTRYAG 5 cut(s) 561, 810, 1197, 2526, 2739
BfoI RGCGCY 2 cut(s) 249, 2416
BfuAI ACCTGC 2 cut(s) 401, 1367
BfuI GTATCC 2 cut(s) 1878, 2694
BglII AGATCT 3 cut(s) 1216, 2161, 2175
BisI GCNGC 8 cut(s) 547, 810, 813, 930, 1197, 2352, 2529, 2739
BlsI GCNGC 8 cut(s) 548, 811, 814, 931, 1198, 2353, 2530, 2740
BmcAI AGTACT 4 cut(s) 1511, 1640, 1862, 2140
Bme1390I CCNGG 6 cut(s) 129, 1627, 2300, 2356, 3045, 3197
Bme18I GGWCC 2 cut(s) 2222, 2908
BmgT120I GGNCC 4 cut(s) 1796, 2222, 2320, 2908
BmrFI CCNGG 6 cut(s) 129, 1627, 2300, 2356, 3045, 3197
BmrI ACTGGG 1 cut(s) 1107
BmsI GCATC 9 cut(s) 190, 1114, 1372, 1970, 2065, 2089, 2110, 2524, 2839
BmtI GCTAGC 1 cut(s) 1256
BmuI ACTGGG 1 cut(s) 1107
BoxI GACNNNNGTC 1 cut(s) 1112
BpiI GAAGAC 4 cut(s) 1010, 1719, 2336, 2583
BpmI CTGGAG 6 cut(s) 405, 562, 2497, 2523, 2690, 3132
Bpu10I CCTNAGC 4 cut(s) 301, 1503, 1752, 2868
BpuEI CTTGAG 2 cut(s) 2370, 2716
BsaHI GRCGYC 1 cut(s) 246
BsaI GGTCTC 1 cut(s) 817
BsaJI CCNNGG 6 cut(s) 128, 1421, 1460, 1625, 1626, 3043
BsaWI WCCGGW 2 cut(s) 2367, 2553
BsaXI ACNNNNNCTCC 2 cut(s) 1844, 1874
Bsc4I CCNNNNNNNGG 8 cut(s) 128, 499, 671, 1427, 1461, 2361, 2404, 3044
Bse3DI GCAATG 4 cut(s) 1689, 1990, 2042, 2762
BseAI TCCGGA 1 cut(s) 2553
BseBI CCWGG 6 cut(s) 129, 1627, 2300, 2356, 3045, 3197
BseDI CCNNGG 6 cut(s) 128, 1421, 1460, 1625, 1626, 3043
BseLI CCNNNNNNNGG 8 cut(s) 128, 499, 671, 1427, 1461, 2361, 2404, 3044
BseMI GCAATG 4 cut(s) 1689, 1990, 2042, 2762
BseRI GAGGAG 1 cut(s) 386
BseSI GKGCMC 2 cut(s) 418, 1992
BseXI GCAGC 7 cut(s) 796, 824, 941, 1183, 2338, 2540, 2725
BsgI GTGCAG 3 cut(s) 207, 705, 2223
Bsh1236I CGCG 1 cut(s) 1618
BshFI GGCC 8 cut(s) 1145, 1797, 1966, 2153, 2321, 2524, 2873, 3200
BshNI GGYRCC 4 cut(s) 245, 757, 1989, 2364
BsiHKAI GWGCWC 1 cut(s) 418
BsiSI CCGG 2 cut(s) 2368, 2554
BslFI GGGAC 4 cut(s) 950, 1100, 1627, 2208
BslI CCNNNNNNNGG 8 cut(s) 128, 499, 671, 1427, 1461, 2361, 2404, 3044
BsmAI GTCTC 4 cut(s) 42, 817, 2720, 2932
BsmFI GGGAC 4 cut(s) 950, 1100, 1627, 2208
BsmI GAATGC 2 cut(s) 194, 1620
BsnI GGCC 8 cut(s) 1145, 1797, 1966, 2153, 2321, 2524, 2873, 3200
Bso31I GGTCTC 1 cut(s) 817
Bsp1286I GDGCHC 4 cut(s) 418, 1992, 2869, 3008
Bsp13I TCCGGA 1 cut(s) 2553
Bsp1407I TGTACA 2 cut(s) 2425, 2604
Bsp19I CCATGG 1 cut(s) 1460
BspACI CCGC 2 cut(s) 547, 3002
BspANI GGCC 8 cut(s) 1145, 1797, 1966, 2153, 2321, 2524, 2873, 3200
BspEI TCCGGA 1 cut(s) 2553
BspFNI CGCG 1 cut(s) 1618
BspHI TCATGA 1 cut(s) 691
BspMAI CTGCAG 4 cut(s) 814, 1201, 2530, 2743
BspMI ACCTGC 2 cut(s) 401, 1367
BspOI GCTAGC 1 cut(s) 1256
BspPI GGATC 1 cut(s) 1905
BspQI GCTCTTC 1 cut(s) 14
BspT107I GGYRCC 4 cut(s) 245, 757, 1989, 2364
BspTNI GGTCTC 1 cut(s) 817
BsrBI CCGCTC 1 cut(s) 549
BsrDI GCAATG 4 cut(s) 1689, 1990, 2042, 2762
BsrGI TGTACA 2 cut(s) 2425, 2604
BssECI CCNNGG 6 cut(s) 128, 1421, 1460, 1625, 1626, 3043
BssNI GRCGYC 1 cut(s) 246
BssSI CACGAG 2 cut(s) 318, 1968
BssT1I CCWWGG 2 cut(s) 1421, 1460
Bst2BI CACGAG 2 cut(s) 318, 1968
Bst2UI CCWGG 6 cut(s) 129, 1627, 2300, 2356, 3045, 3197
Bst4CI ACNGT 6 cut(s) 333, 471, 1353, 2085, 2399, 2747
Bst6I CTCTTC 2 cut(s) 14, 2949
BstACI GRCGYC 1 cut(s) 246
BstAPI GCANNNNNTGC 3 cut(s) 1382, 1996, 2534
BstAUI TGTACA 2 cut(s) 2425, 2604
BstDSI CCRYGG 1 cut(s) 1460
BstENI CCTNNNNNAGG 1 cut(s) 126
BstFNI CGCG 1 cut(s) 1618
BstH2I RGCGCY 2 cut(s) 249, 2416
BstHHI GCGC 3 cut(s) 248, 1620, 2415
BstMAI GTCTC 4 cut(s) 42, 817, 2720, 2932
BstMWI GCNNNNNNNGC 7 cut(s) 1382, 1417, 1615, 1996, 2150, 2534, 2821
BstNI CCWGG 6 cut(s) 129, 1627, 2300, 2356, 3045, 3197
BstNSI RCATGY 4 cut(s) 194, 289, 1452, 1720
BstPAI GACNNNNGTC 1 cut(s) 1112
BstSCI CCNGG 6 cut(s) 127, 1625, 2298, 2354, 3043, 3195
BstSFI CTRYAG 5 cut(s) 561, 810, 1197, 2526, 2739
BstSLI GKGCMC 2 cut(s) 418, 1992
BstUI CGCG 1 cut(s) 1618
BstV1I GCAGC 7 cut(s) 796, 824, 941, 1183, 2338, 2540, 2725
BstV2I GAAGAC 4 cut(s) 1010, 1719, 2336, 2583
BstX2I RGATCY 4 cut(s) 1216, 1897, 2161, 2175
BstXI CCANNNNNNTGG 1 cut(s) 1675
BstYI RGATCY 4 cut(s) 1216, 1897, 2161, 2175
BsuI GTATCC 2 cut(s) 1878, 2694
BsuRI GGCC 8 cut(s) 1145, 1797, 1966, 2153, 2321, 2524, 2873, 3200
BtgI CCRYGG 1 cut(s) 1460
BtsI GCAGTG 1 cut(s) 510
BveI ACCTGC 2 cut(s) 401, 1367
CciI TCATGA 1 cut(s) 691
CfoI GCGC 3 cut(s) 248, 1620, 2415
Cfr13I GGNCC 4 cut(s) 1796, 2222, 2320, 2908
CseI GACGC 1 cut(s) 1624
Csp6I GTAC 9 cut(s) 1095, 1452, 1465, 1510, 1639, 1861, 2139, 2426, 2605
CspCI CAANNNNNGTGG 4 cut(s) 56, 91, 1290, 1325
CviQI GTAC 9 cut(s) 1095, 1452, 1465, 1510, 1639, 1861, 2139, 2426, 2605
DinI GGCGCC 1 cut(s) 247
DraI TTTAAA 1 cut(s) 1158
EaeI YGGCCR 1 cut(s) 3198
Eam1104I CTCTTC 2 cut(s) 14, 2949
EarI CTCTTC 2 cut(s) 14, 2949
Eco130I CCWWGG 2 cut(s) 1421, 1460
Eco147I AGGCCT 1 cut(s) 2873
Eco24I GRGCYC 2 cut(s) 2869, 3008
Eco31I GGTCTC 1 cut(s) 817
Eco32I GATATC 1 cut(s) 3103
Eco47I GGWCC 2 cut(s) 2222, 2908
EcoNI CCTNNNNNAGG 1 cut(s) 126
EcoO109I RGGNCCY 3 cut(s) 2222, 2320, 2908
EcoRI GAATTC 2 cut(s) 458, 1316
EcoRII CCWGG 6 cut(s) 127, 1625, 2298, 2354, 3043, 3195
EcoRV GATATC 1 cut(s) 3103
EcoT14I CCWWGG 2 cut(s) 1421, 1460
EcoT22I ATGCAT 2 cut(s) 196, 856
EcoT38I GRGCYC 2 cut(s) 2869, 3008
EgeI GGCGCC 1 cut(s) 247
EheI GGCGCC 1 cut(s) 247
ErhI CCWWGG 2 cut(s) 1421, 1460
FalI AAGNNNNNCTT 4 cut(s) 789, 821, 2934, 2966
FaqI GGGAC 4 cut(s) 950, 1100, 1627, 2208
FauI CCCGC 1 cut(s) 2995
FauNDI CATATG 1 cut(s) 1474
Fnu4HI GCNGC 8 cut(s) 547, 810, 813, 930, 1197, 2352, 2529, 2739
FriOI GRGCYC 2 cut(s) 2869, 3008
Fsp4HI GCNGC 8 cut(s) 547, 810, 813, 930, 1197, 2352, 2529, 2739
FspBI CTAG 5 cut(s) 212, 960, 1253, 2136, 2159
GlaI GCGC 3 cut(s) 247, 1619, 2414
GluI GCNGC 8 cut(s) 547, 810, 813, 930, 1197, 2352, 2529, 2739
GsuI CTGGAG 6 cut(s) 405, 562, 2497, 2523, 2690, 3132
HaeII RGCGCY 2 cut(s) 249, 2416
HaeIII GGCC 8 cut(s) 1145, 1797, 1966, 2153, 2321, 2524, 2873, 3200
HapII CCGG 2 cut(s) 2368, 2554
HgaI GACGC 1 cut(s) 1624
HhaI GCGC 3 cut(s) 248, 1620, 2415
Hin1I GRCGYC 1 cut(s) 246
Hin6I GCGC 3 cut(s) 246, 1618, 2413
HinP1I GCGC 3 cut(s) 246, 1618, 2413
HincII GTYRAC 2 cut(s) 1074, 1272
HindII GTYRAC 2 cut(s) 1074, 1272
HindIII AAGCTT 2 cut(s) 311, 2813
HpaII CCGG 2 cut(s) 2368, 2554
HphI GGTGA 6 cut(s) 719, 1391, 1442, 2117, 2653, 3188
Hpy166II GTNNAC 8 cut(s) 94, 416, 842, 1074, 1272, 1400, 2025, 2456
Hpy188I TCNGA 9 cut(s) 47, 924, 1050, 1123, 1537, 1660, 1816, 1836, 2315
Hpy8I GTNNAC 8 cut(s) 94, 416, 842, 1074, 1272, 1400, 2025, 2456
HpyCH4III ACNGT 6 cut(s) 333, 471, 1353, 2085, 2399, 2747
HpyCH4IV ACGT 4 cut(s) 226, 646, 2210, 2434
HpyF10VI GCNNNNNNNGC 7 cut(s) 1382, 1417, 1615, 1996, 2150, 2534, 2821
HpySE526I ACGT 4 cut(s) 226, 646, 2210, 2434
Hsp92I GRCGYC 1 cut(s) 246
HspAI GCGC 3 cut(s) 246, 1618, 2413
KasI GGCGCC 1 cut(s) 245
Kpn2I TCCGGA 1 cut(s) 2553
LguI GCTCTTC 1 cut(s) 14
LmnI GCTCC 9 cut(s) 373, 543, 554, 881, 1132, 1359, 2449, 2482, 2542
Lsp1109I GCAGC 7 cut(s) 796, 824, 941, 1183, 2338, 2540, 2725
LweI GCATC 9 cut(s) 190, 1114, 1372, 1970, 2065, 2089, 2110, 2524, 2839
MaeI CTAG 5 cut(s) 212, 960, 1253, 2136, 2159
MaeII ACGT 4 cut(s) 226, 646, 2210, 2434
MaeIII GTNAC 8 cut(s) 362, 536, 556, 647, 1105, 1744, 1821, 3205
MbiI CCGCTC 1 cut(s) 549
MfeI CAATTG 2 cut(s) 2055, 2195
MflI RGATCY 4 cut(s) 1216, 1897, 2161, 2175
MhlI GDGCHC 4 cut(s) 418, 1992, 2869, 3008
MlsI TGGCCA 1 cut(s) 3200
MluNI TGGCCA 1 cut(s) 3200
Mly113I GGCGCC 1 cut(s) 246
MlyI GAGTC 3 cut(s) 972, 2722, 3331
MmeI TCCRAC 1 cut(s) 3077
Mox20I TGGCCA 1 cut(s) 3200
Mph1103I ATGCAT 2 cut(s) 196, 856
MroI TCCGGA 1 cut(s) 2553
MroXI GAANNNNTTC 4 cut(s) 488, 714, 800, 2714
MscI TGGCCA 1 cut(s) 3200
MslI CAYNNNNRTG 4 cut(s) 189, 284, 743, 1673
Msp20I TGGCCA 1 cut(s) 3200
MspA1I CMGCKG 2 cut(s) 305, 2531
MspI CCGG 2 cut(s) 2368, 2554
MspR9I CCNGG 6 cut(s) 129, 1627, 2300, 2356, 3045, 3197
MunI CAATTG 2 cut(s) 2055, 2195
Mva1269I GAATGC 2 cut(s) 194, 1620
MvaI CCWGG 6 cut(s) 129, 1627, 2300, 2356, 3045, 3197
MvnI CGCG 1 cut(s) 1618
MwoI GCNNNNNNNGC 7 cut(s) 1382, 1417, 1615, 1996, 2150, 2534, 2821
NarI GGCGCC 1 cut(s) 246
NcoI CCATGG 1 cut(s) 1460
NdeI CATATG 1 cut(s) 1474
NheI GCTAGC 1 cut(s) 1252
NmuCI GTSAC 5 cut(s) 362, 536, 1105, 1821, 3205
NsiI ATGCAT 2 cut(s) 196, 856
NspI RCATGY 4 cut(s) 194, 289, 1452, 1720
PaeI GCATGC 1 cut(s) 289
PagI TCATGA 1 cut(s) 691
PaqCI CACCTGC 2 cut(s) 401, 1367
PasI CCCWGGG 1 cut(s) 1626
PceI AGGCCT 1 cut(s) 2873
PciI ACATGT 1 cut(s) 1716
PciSI GCTCTTC 1 cut(s) 14
PctI GAATGC 2 cut(s) 194, 1620
PdmI GAANNNNTTC 4 cut(s) 488, 714, 800, 2714
PfeI GAWTC 8 cut(s) 64, 104, 571, 1579, 1649, 1661, 1909, 2003
PflFI GACNNNGTC 2 cut(s) 2246, 2747
PflMI CCANNNNNTGG 3 cut(s) 671, 2361, 3044
PkrI GCNGC 8 cut(s) 548, 811, 814, 931, 1198, 2353, 2530, 2740
PleI GAGTC 3 cut(s) 971, 2722, 3330
PluTI GGCGCC 1 cut(s) 249
PpsI GAGTC 3 cut(s) 971, 2722, 3330
PpuMI RGGWCCY 2 cut(s) 2222, 2908
PscI ACATGT 1 cut(s) 1716
PshAI GACNNNNGTC 1 cut(s) 1112
PshBI ATTAAT 1 cut(s) 1829
PsiI TTATAA 1 cut(s) 3228
Psp1406I AACGTT 1 cut(s) 646
Psp5II RGGWCCY 2 cut(s) 2222, 2908
Psp6I CCWGG 6 cut(s) 127, 1625, 2298, 2354, 3043, 3195
PspGI CCWGG 6 cut(s) 127, 1625, 2298, 2354, 3043, 3195
PspPI GGNCC 4 cut(s) 1796, 2222, 2320, 2908
PspPPI RGGWCCY 2 cut(s) 2222, 2908
PstI CTGCAG 4 cut(s) 814, 1201, 2530, 2743
PsuI RGATCY 4 cut(s) 1216, 1897, 2161, 2175
PsyI GACNNNGTC 2 cut(s) 2246, 2747
PvuII CAGCTG 2 cut(s) 305, 2531
RsaI GTAC 9 cut(s) 1096, 1453, 1466, 1511, 1640, 1862, 2140, 2427, 2606
RsaNI GTAC 9 cut(s) 1095, 1452, 1465, 1510, 1639, 1861, 2139, 2426, 2605
RseI CAYNNNNRTG 4 cut(s) 189, 284, 743, 1673
SapI GCTCTTC 1 cut(s) 14
SatI GCNGC 8 cut(s) 547, 810, 813, 930, 1197, 2352, 2529, 2739
Sau96I GGNCC 4 cut(s) 1796, 2222, 2320, 2908
ScaI AGTACT 4 cut(s) 1511, 1640, 1862, 2140
SchI GAGTC 3 cut(s) 972, 2722, 3331
ScrFI CCNGG 6 cut(s) 129, 1627, 2300, 2356, 3045, 3197
SduI GDGCHC 4 cut(s) 418, 1992, 2869, 3008
SfaNI GCATC 9 cut(s) 190, 1114, 1372, 1970, 2065, 2089, 2110, 2524, 2839
SfcI CTRYAG 5 cut(s) 561, 810, 1197, 2526, 2739
SfoI GGCGCC 1 cut(s) 247
SinI GGWCC 2 cut(s) 2222, 2908
SmiMI CAYNNNNRTG 4 cut(s) 189, 284, 743, 1673
SmlI CTYRAG 2 cut(s) 2385, 2695
SmoI CTYRAG 2 cut(s) 2385, 2695
SphI GCATGC 1 cut(s) 289
SseBI AGGCCT 1 cut(s) 2873
SsiI CCGC 2 cut(s) 547, 3002
SspDI GGCGCC 1 cut(s) 245
SspI AATATT 3 cut(s) 2066, 2269, 3143
SspMI CTAG 5 cut(s) 212, 960, 1253, 2136, 2159
StuI AGGCCT 1 cut(s) 2873
StyD4I CCNGG 6 cut(s) 127, 1625, 2298, 2354, 3043, 3195
StyI CCWWGG 2 cut(s) 1421, 1460
TaaI ACNGT 6 cut(s) 333, 471, 1353, 2085, 2399, 2747
TaiI ACGT 4 cut(s) 229, 649, 2213, 2437
TaqI TCGA 2 cut(s) 1647, 1882
TatI WGTACW 6 cut(s) 1509, 1638, 1860, 2138, 2425, 2604
TauI GCSGC 1 cut(s) 549
TfiI GAWTC 8 cut(s) 64, 104, 571, 1579, 1649, 1661, 1909, 2003
TseFI GTSAC 5 cut(s) 362, 536, 1105, 1821, 3205
TseI GCWGC 7 cut(s) 809, 812, 929, 1196, 2351, 2528, 2738
Tsp45I GTSAC 5 cut(s) 362, 536, 1105, 1821, 3205
TspGWI ACGGA 1 cut(s) 1338
Tth111I GACNNNGTC 2 cut(s) 2246, 2747
Van91I CCANNNNNTGG 3 cut(s) 671, 2361, 3044
VneI GTGCAC 1 cut(s) 414
VpaK11BI GGWCC 2 cut(s) 2222, 2908
VspI ATTAAT 1 cut(s) 1829
XagI CCTNNNNNAGG 1 cut(s) 126
XapI RAATTY 5 cut(s) 37, 458, 1316, 2125, 2623
XbaI TCTAGA 1 cut(s) 959
XceI RCATGY 4 cut(s) 194, 289, 1452, 1720
XcmI CCANNNNNNNNNTGG 2 cut(s) 1672, 2569
XmnI GAANNNNTTC 4 cut(s) 488, 714, 800, 2714
XspI CTAG 5 cut(s) 212, 960, 1253, 2136, 2159
ZrmI AGTACT 4 cut(s) 1511, 1640, 1862, 2140
Zsp2I ATGCAT 2 cut(s) 196, 856
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.