MD15G1439400.v1.1

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000598
Physical Location & Seq
Forward (+)
53947964 .. 53955064
7101 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1439400.v1.1.491

Sequence Viewer

Length: 3291 bp
ATGGCGGCTGAAGGTGGTGGGAGGAGCTTCGCCAGGAGAGACCACCTCCTCGAGATTGAGACAAAGGTTCGAAGCTGGTGGGAAGAGAAGGATGTTTTCAGAGCTGAATCTTGTGAAAAGCCTCCCGAACCTGGGGAGAAGTTCTTTGGAAACTTTCCATTTCCGTACATGAATGGTTTTTTGCATCTTGGACATGCATTCTCACTCTCCAAGCTAGAGTTTGCTGCAGCTTACCATAGGTTAAGAGGTGCCAATGTGCTTTTGCCTTTCGCTTTTCACTGCACTGGCATGCCTATCAAAGCGTCGGCTGATAAACTTGCGAGAGAGATTCAACTGTTTGGCGACCCTCCAGTTTTTCCAAAAGAAGTGGAACAGGGGAGTCAGGAAGTTGAAGCAGAAACAGAAGGTGCAAATAGTGGAGCACCTCCAGATAAGTTCAAGGGAAAGAAGTCGAAGGCTGCATCAAAATCAGGTGGACAAGCATACCAATGGGAAATCATGCGTAGTTTTGGCCTCTCTGATAGCGAGATATCCAAATTCCAAAATCCATACAACTGGTTGACATTCTTTCCTCCATTGGCAGTGGAAGACCTCAAGGCTTTTGGATTGGGATGTGATTGGAGGCGGTCATTTATTACTACAGACATGAACCCAGTTTTTGATGCCTTTGTGAGGTGGCAGGTGAGGAAATTAAAATCCATGGGCAAGATTGTGAAAGATGTCCGTTACGCAATTTACTCTCCATTGGATGGGCAGCCATGTGCAGATCATGACAGGGCAAGTGGCGAAGGTGTTCAGCCCCAGGAATACACCCTTATCAAAATGGAGGTGGTCGCACCTTTCCCGTCTAAACTGAAAGTGTTGGATGGAAGGAAAGTGTTCCTTGCTGCAGCAACACTGAGACCTGAGACCATGTATGGACAAACAAATGCATGGGTATTGCCTGATGGGAAATATGGAGCCTTTGAAATCAATGAAACAGAGGTACTTATTCTAACGCAGAGAGCAGCACTTAATCTTGCCTATCAGAAGTATTCTAGGGTCCCAGAGAAACCTACTTGTTTGGTGGAGCTCACAGGTCGTGATTTGATTGGCCTTCCATTGAAGTCACCACGCGCAATCAATCAGATTATTTACACTCTTCCTATGTTGACTGTCCTGACAGACAAAGGTACTGGGATTGTGACCAGCGTGCCTGCTGATTCTCCTGATGATTATATGGCCTTACATGATTTAAAAGCAAAACCCGCTCTTAGAGCAAAATATGGCGTGAAGGATGAGTGGGTCATGCCCTTTGAGATTATTCCAATTATCAATATTCCTGAATTCGGAAATAAGGCTGCGGAAAAGGTCTGTGCAGATCTAAAAATCAAGAGCCAGAATGAAAAAGAGAAGCTAGCAGAAGCCAAGAGACTGACATACCTGAAAGGATTCACTGAGGGAACACTTATTGTGGGTGAATTCGAAGGATGGAAAGTCCAGGAGGCGAAACCGTTGATAAGGAGCAAGCTCATAGAGGCAAATGAAGCAATTGTGTATAGTGAACCTGAGAAGCGGGTGGTGTCACGATCAGGTGATGAATGTGTTGTGGCTCTCACAGATCAATGGTACATCACATATGGGGAACCTGAATGGAAAAAACTGGCTGAGGAGTGCTTGTCCAGCATGAATTTGTATCATGACGAGACAAGACATGGCTTTGAACACACATTGGGTTGGTTGAATCAATGGGCTTGTTCACGATCTTTTGGGCTTGGGACTCGTATTCCCTGGGATGAAGAGTTCTTAGTTGAGTCGTTGTCTGATTCAACTATTTACATGGCTTATTACACTATTGCTCACTTTTTACACAATGGAGACATGTATGGTTCCAGCAAATCTGCAATTAAACCTGAACAAATGACTGATGAGGTCTGGGAGTATATTTTCTGTGATGGCCCTTATCCTGAATCATCTGACATCTCGTCATCCATTCTTAATAAGATGAAGCAGGAGTTTGAGTATTGGTATCCTTTTGATCTTCGAGTGTCTGGCAAGGATCTAATCCAAAATCATTTAACCTTCTGCATTTACAACCACACGGCAATTATGGCCAAGAAGCACTGGCCTTGTGGGTTTAGATGCAATGGGCACATCATGTTAAATGCTGAGAAAATGTCCAAGTCTACAGGGAACTTTAGGACCATTCGCCAGGCAATTGAGGAATTTTCTGCTGATGCCACGCGATTCTCATTGGCTGATGCTGGAGATGGTGTTGATGATGCAAATTTCGTATTTGAGACTGCAAATGCTGCTATTCTAAAGCTAACTAAAGAGATCGCATGGATGGAAGAAGTTTTAGCTGCAGAATCCTCTTTGAGAATAGGTCCTCCTTCTACTTATGCTGACAGGGTCTTTTTGAACGAGATAAATATTGCTGTGAACAGGACTGAGCAGAATTACCGGGATTACATGTTCCGAGATGCGCTCAAGACTGGGTTTTACGATCTTCAGGCTTCTAGGGATGAGTACAGGCTTTCATGTGGTTCTGGGGGTATGAACCGTGAATTGCTGTGGCGTTTTATGGATGTGCAGACACGTCTCATTACCCCAATCTGTCCACACTATGCCGAATATGTCTGGAGGGAACTTTTGAAGAAGGAAGGGTTTGTGGTTAATGCAGGATGGCCTGTGGCTGATTCTCCAGATCTGATCCTCCAGAGTGCCAACAAGTATTTGCAACATTCCATTGTTGTTATGAGGAAGCTTCTTGAAAAGCAAACTTCAGGCTCAACAAAAGGCAATAAGAAGGGTCCTCCAGCTAAAACCTCGACGGAAACCAAAAGGTTGATTGGTTTGATATATGTAAATGAGCAATTCGATGGATGGAAGGCTGAGTGTTTGAGGATACTACAAAGTAACTTTGACCAAGATACTCGCACTTTTGCTTCAGACAAGGTAATTCTGGAGGCTTTGCAAAGAAGTTCAATTGGTCAGAGTAAAGATTTTAGACAAACACAAAAGCTTTGTATGCCTTTTTTGAGGTTCAAGAAGGATGAGGCGGTTACTCTTGGGGCCCATGCCTTAGACTTGAAGCTACCATTTGGAGAGATTGAGGTCCTTCAAGAGAACTTGGACTTAATTAAGAGACAAGTGAAGGTGGACACTAAGGGACAAGTCGAGCTTGAAGAAGTAGAAGTTCTGTCTGGTACTGACCCTGATGCTCTTGCTAAAGCTGGTTCACTTGTTAAATTAATAGAGCAGAATCCGCCGTCCCCTGGAAGCCCAACTGCCATCTTCTTGACCCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

1097

Amino Acids

123.9

Weight (kDa)

5.96

Isoelectric Point (pI)

48.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1 PF00133 24 - 105 1.8e-08 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1 PF00133 196 - 756 7.1e-15 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g PF09334 663 - 763 5.9e-08 tRNA synthetases class I (M)
tRNA-synt_1e PF01406 670 - 746 5e-08 tRNA synthetases class I (C) catalytic domain
Anticodon_1 PF08264 796 - 920 1.7e-15 Anticodon-binding domain of tRNA ligase
RBD_LARS1 PF24810 950 - 1026 6.2e-07 Leucine--tRNA ligase, RagD-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000598)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09620
fragaria_vesca FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_3g34581 FvH4_4g33720 FvH4_4g33720
malus_domestica MD08G1244600.v1.1 MD15G1439400.v1.1
prunus_persica Prupe.1G582000_v2.0.a1 Prupe.2G015100_v2.0.a1 Prupe.2G123100_v2.0.a1
pyrus_communis pycom08g21210 pycom08g21220 pycom15g38730
rosa_chinensis RchiOBHm_Chr1g0336121 RchiOBHm_Chr1g0353811 RchiOBHm_Chr1g0373331 RchiOBHm_Chr4g0443191 RchiOBHm_Chr4g0443201 RchiOBHm_Chr4g0443211
rosa_laevigata RLG00000003122 RLG00000005948 RLG00000026826 RLG00000028251 RLG00000028252 RLG00000028253 RLG00000029377
rosa_multiflora Rmu_co8002498.1_g000001 Rmu_co8262279.1_g000001 Rmu_co8314139.1_g000001 Rmu_co8393711.1_g000001 Rmu_co8419167.1_g000001 Rmu_sc0007968.1_g000004 Rmu_sc0018919.1_g000004 Rmu_sc0027447.1_g000001
rosa_roxburghii Rroxscaffold_4G00284260 Rroxscaffold_4G00301490 Rroxscaffold_4G00315860 Rroxscaffold_5G00383790 Rroxscaffold_5G00383800
rosa_rugosa Rorug01G0123100 Rorug01G0238700 Rorug01G0377100 Rorug01G0377100 Rorug01G0377200 Rorug01G0377300 Rorug04G0344700 Rorug05G0347900.1
rosa_samantha Rh1AG143800 Rh1AG250300 Rh1AG386300 Rh1BG113400 Rh1BG220800 Rh1BG220900 Rh1BG350400 Rh1CG135500 Rh1CG233100 Rh1CG363500 Rh1DG150200 Rh1DG247700 Rh1DG247800 Rh1DG381300 Rh4AG399400 Rh4AG399500 Rh4BG411000 Rh4BG411100 Rh4BG411200 Rh4CG426500 Rh4CG426600 Rh4DG406100 Rh4DG406200 Rh4DG406300
rosa_wichuraiana Rw0G015190 Rw1G021850 Rw1G034870 Rw4G034440 Rw4G034450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 670
Acc36I ACCTGC 1 cut(s) 670
AccB1I GGYRCC 1 cut(s) 248
AccB7I CCANNNNNTGG 1 cut(s) 749
AccBSI CCGCTC 1 cut(s) 1250
AccI GTMKAC 1 cut(s) 2165
AccII CGCG 2 cut(s) 1116, 2224
AciI CCGC 7 cut(s) 5, 625, 1248, 1343, 1555, 3041, 3248
AclWI GGATC 2 cut(s) 2046, 2686
AcoI YGGCCR 1 cut(s) 2091
AcsI RAATTY 6 cut(s) 536, 1325, 1460, 1669, 2204, 2266
AcuI CTGAAG 4 cut(s) 30, 2474, 2748, 2913
AfaI GTAC 6 cut(s) 167, 987, 1174, 1610, 2510, 3190
AfiI CCNNNNNNNGG 6 cut(s) 131, 132, 672, 749, 1328, 3257
AflIII ACRYGT 3 cut(s) 1860, 2451, 2576
AhdI GACNNNNNGTC 1 cut(s) 1963
AjiI CACGTC 1 cut(s) 2579
AjnI CCWGG 7 cut(s) 32, 130, 801, 1479, 1769, 2190, 3256
AloI GAACNNNNNNTCC 4 cut(s) 1766, 1798, 2438, 2470
Alw21I GWGCWC 2 cut(s) 424, 1074
AlwI GGATC 2 cut(s) 2046, 2686
Ama87I CYCGRG 1 cut(s) 50
AoxI GGCC 8 cut(s) 511, 1093, 1221, 1936, 2091, 2105, 2666, 3054
ApaI GGGCCC 1 cut(s) 3058
ApoI RAATTY 6 cut(s) 536, 1325, 1460, 1669, 2204, 2266
AseI ATTAAT 1 cut(s) 3233
Asp700I GAANNNNTTC 3 cut(s) 792, 878, 1430
AspLEI GCGC 2 cut(s) 1118, 2467
AspS9I GGNCC 8 cut(s) 1042, 1937, 2181, 2366, 2792, 3054, 3055, 3097
AsuC2I CCSGG 2 cut(s) 2444, 3286
AsuHPI GGTGA 4 cut(s) 694, 1101, 1469, 1586
AsuII TTCGAA 2 cut(s) 70, 1464
AsuNHI GCTAGC 1 cut(s) 1396
AvaI CYCGRG 1 cut(s) 50
AvaII GGWCC 5 cut(s) 1042, 2181, 2366, 2792, 3097
BaeGI GKGCMC 2 cut(s) 2133, 3058
BalI TGGCCA 1 cut(s) 2093
BanI GGYRCC 1 cut(s) 248
BanII GRGCYC 2 cut(s) 1074, 3058
BbsI GAAGAC 1 cut(s) 594
Bbv12I GWGCWC 2 cut(s) 424, 1074
BbvCI CCTCAGC 1 cut(s) 1647
BceAI ACGGC 2 cut(s) 2097, 3235
BciT130I CCWGG 7 cut(s) 34, 132, 803, 1481, 1771, 2192, 3258
BciVI GTATCC 2 cut(s) 2019, 2880
BcnI CCSGG 2 cut(s) 2444, 3286
BfaI CTAG 4 cut(s) 215, 1038, 1397, 2499
BfmI CTRYAG 5 cut(s) 225, 639, 888, 2166, 2343
BfuAI ACCTGC 1 cut(s) 670
BfuI GTATCC 2 cut(s) 2019, 2880
BglII AGATCT 2 cut(s) 1360, 2686
Bme1390I CCNGG 9 cut(s) 34, 132, 803, 1481, 1771, 2192, 2444, 3258, 3286
Bme18I GGWCC 5 cut(s) 1042, 2181, 2366, 2792, 3097
BmeRI GACNNNNNGTC 1 cut(s) 1963
BmeT110I CYCGRG 1 cut(s) 50
BmgBI CACGTC 1 cut(s) 2579
BmgT120I GGNCC 8 cut(s) 1042, 1937, 2181, 2366, 2792, 3054, 3055, 3097
BmiI GGNNCC 9 cut(s) 250, 961, 1043, 1044, 1626, 1870, 2793, 3055, 3056
BmrFI CCNGG 9 cut(s) 34, 132, 803, 1481, 1771, 2192, 2444, 3258, 3286
BmrI ACTGGG 3 cut(s) 647, 1185, 2484
BmsI GCATC 9 cut(s) 193, 470, 652, 2111, 2206, 2230, 2251, 2452, 3190
BmtI GCTAGC 1 cut(s) 1400
BmuI ACTGGG 3 cut(s) 647, 1185, 2484
BpiI GAAGAC 1 cut(s) 594
BplI GAGNNNNNCTC 4 cut(s) 30, 62, 2451, 2483
BpmI CTGGAG 8 cut(s) 333, 411, 2265, 2641, 2667, 2681, 2781, 2966
Bpu10I CCTNAGC 1 cut(s) 1647
Bpu14I TTCGAA 2 cut(s) 70, 1464
BpuEI CTTGAG 2 cut(s) 578, 2453
BpuMI CCSGG 2 cut(s) 2444, 3286
BsaI GGTCTC 3 cut(s) 33, 895, 902
BsaJI CCNNGG 6 cut(s) 131, 699, 801, 1769, 1770, 3256
BsaXI ACNNNNNCTCC 6 cut(s) 1643, 1673, 1985, 2015, 2238, 2268
Bsc4I CCNNNNNNNGG 6 cut(s) 131, 132, 672, 749, 1328, 3257
Bse1I ACTGG 8 cut(s) 289, 350, 560, 653, 1180, 1647, 2108, 2479
Bse3DI GCAATG 1 cut(s) 2131
BseBI CCWGG 7 cut(s) 34, 132, 803, 1481, 1771, 2192, 3258
BseDI CCNNGG 6 cut(s) 131, 699, 801, 1769, 1770, 3256
BseLI CCNNNNNNNGG 6 cut(s) 131, 132, 672, 749, 1328, 3257
BseMI GCAATG 1 cut(s) 2131
BseMII CTCAG 8 cut(s) 890, 897, 1428, 1539, 1638, 2139, 2421, 2865
BseNI ACTGG 8 cut(s) 289, 350, 560, 653, 1180, 1647, 2108, 2479
BseRI GAGGAG 3 cut(s) 37, 38, 1664
BseSI GKGCMC 2 cut(s) 2133, 3058
BsgI GTGCAG 4 cut(s) 265, 783, 1377, 2591
Bsh1236I CGCG 2 cut(s) 1116, 2224
BshFI GGCC 8 cut(s) 513, 1095, 1223, 1938, 2093, 2107, 2668, 3056
BshNI GGYRCC 1 cut(s) 248
BsiHKAI GWGCWC 2 cut(s) 424, 1074
BsiHKCI CYCGRG 1 cut(s) 50
BsiSI CCGG 2 cut(s) 2443, 3286
BslFI GGGAC 4 cut(s) 1028, 1771, 3165, 3238
BslI CCNNNNNNNGG 6 cut(s) 131, 132, 672, 749, 1328, 3257
BsmBI CGTCTC 1 cut(s) 2585
BsmFI GGGAC 4 cut(s) 1028, 1771, 3165, 3238
BsmI GAATGC 1 cut(s) 197
BsnI GGCC 8 cut(s) 513, 1095, 1223, 1938, 2093, 2107, 2668, 3056
Bso31I GGTCTC 3 cut(s) 33, 895, 902
BsoBI CYCGRG 1 cut(s) 50
Bsp119I TTCGAA 2 cut(s) 70, 1464
Bsp120I GGGCCC 1 cut(s) 3054
Bsp1286I GDGCHC 4 cut(s) 424, 1074, 2133, 3058
Bsp19I CCATGG 1 cut(s) 699
BspACI CCGC 7 cut(s) 5, 625, 1248, 1343, 1555, 3041, 3248
BspANI GGCC 8 cut(s) 513, 1095, 1223, 1938, 2093, 2107, 2668, 3056
BspCNI CTCAG 8 cut(s) 891, 898, 1429, 1540, 1639, 2140, 2422, 2866
BspFNI CGCG 2 cut(s) 1116, 2224
BspHI TCATGA 2 cut(s) 769, 1678
BspLI GGNNCC 9 cut(s) 250, 961, 1043, 1044, 1626, 1870, 2793, 3055, 3056
BspMAI CTGCAG 3 cut(s) 229, 892, 2347
BspMI ACCTGC 1 cut(s) 670
BspOI GCTAGC 1 cut(s) 1400
BspPI GGATC 2 cut(s) 2046, 2686
BspT104I TTCGAA 2 cut(s) 70, 1464
BspT107I GGYRCC 1 cut(s) 248
BspTNI GGTCTC 3 cut(s) 33, 895, 902
BsrBI CCGCTC 1 cut(s) 1250
BsrDI GCAATG 1 cut(s) 2131
BsrI ACTGG 8 cut(s) 289, 350, 560, 653, 1180, 1647, 2108, 2479
BssECI CCNNGG 6 cut(s) 131, 699, 801, 1769, 1770, 3256
BssT1I CCWWGG 1 cut(s) 699
Bst2UI CCWGG 7 cut(s) 34, 132, 803, 1481, 1771, 2192, 3258
Bst4CI ACNGT 4 cut(s) 336, 1156, 1494, 2543
Bst6I CTCTTC 3 cut(s) 78, 1146, 1773
BstAPI GCANNNNNTGC 1 cut(s) 2291
BstBI TTCGAA 2 cut(s) 70, 1464
BstC8I GCNNGC 5 cut(s) 290, 1193, 1197, 1398, 1508
BstDSI CCRYGG 1 cut(s) 699
BstENI CCTNNNNNAGG 1 cut(s) 670
BstFNI CGCG 2 cut(s) 1116, 2224
BstHHI GCGC 2 cut(s) 1118, 2467
BstMWI GCNNNNNNNGC 8 cut(s) 1247, 1256, 1526, 1662, 2090, 2291, 3010, 3247
BstNI CCWGG 7 cut(s) 34, 132, 803, 1481, 1771, 2192, 3258
BstNSI RCATGY 4 cut(s) 197, 292, 1864, 2455
BstSCI CCNGG 9 cut(s) 32, 130, 801, 1479, 1769, 2190, 2442, 3256, 3284
BstSFI CTRYAG 5 cut(s) 225, 639, 888, 2166, 2343
BstSLI GKGCMC 2 cut(s) 2133, 3058
BstUI CGCG 2 cut(s) 1116, 2224
BstV2I GAAGAC 1 cut(s) 594
BstX2I RGATCY 3 cut(s) 1360, 2038, 2686
BstXI CCANNNNNNTGG 1 cut(s) 555
BstYI RGATCY 3 cut(s) 1360, 2038, 2686
BsuI GTATCC 2 cut(s) 2019, 2880
BsuRI GGCC 8 cut(s) 513, 1095, 1223, 1938, 2093, 2107, 2668, 3056
BtgI CCRYGG 1 cut(s) 699
BtrI CACGTC 1 cut(s) 2579
BtsI GCAGTG 2 cut(s) 277, 588
BtsIMutI CAGTG 6 cut(s) 277, 282, 588, 896, 1434, 2101
BveI ACCTGC 1 cut(s) 670
Cac8I GCNNGC 5 cut(s) 290, 1193, 1197, 1398, 1508
CciI TCATGA 2 cut(s) 769, 1678
CfoI GCGC 2 cut(s) 1118, 2467
Cfr13I GGNCC 8 cut(s) 1042, 1937, 2181, 2366, 2792, 3054, 3055, 3097
CseI GACGC 1 cut(s) 291
Csp6I GTAC 6 cut(s) 166, 986, 1173, 1609, 2509, 3189
CspCI CAANNNNNGTGG 2 cut(s) 348, 383
CviQI GTAC 6 cut(s) 166, 986, 1173, 1609, 2509, 3189
DraI TTTAAA 1 cut(s) 1236
DriI GACNNNNNGTC 1 cut(s) 1963
EaeI YGGCCR 1 cut(s) 2091
Eam1104I CTCTTC 3 cut(s) 78, 1146, 1773
Eam1105I GACNNNNNGTC 1 cut(s) 1963
EarI CTCTTC 3 cut(s) 78, 1146, 1773
EciI GGCGGA 1 cut(s) 3237
Ecl136II GAGCTC 1 cut(s) 1072
Eco130I CCWWGG 1 cut(s) 699
Eco24I GRGCYC 2 cut(s) 1074, 3058
Eco31I GGTCTC 3 cut(s) 33, 895, 902
Eco32I GATATC 1 cut(s) 531
Eco47I GGWCC 5 cut(s) 1042, 2181, 2366, 2792, 3097
Eco53kI GAGCTC 1 cut(s) 1072
Eco57I CTGAAG 4 cut(s) 30, 2474, 2748, 2913
Eco88I CYCGRG 1 cut(s) 50
EcoICRI GAGCTC 1 cut(s) 1072
EcoNI CCTNNNNNAGG 1 cut(s) 670
EcoO109I RGGNCCY 5 cut(s) 1042, 2366, 2792, 3054, 3097
EcoRI GAATTC 2 cut(s) 1325, 1460
EcoRII CCWGG 7 cut(s) 32, 130, 801, 1479, 1769, 2190, 3256
EcoRV GATATC 1 cut(s) 531
EcoT14I CCWWGG 1 cut(s) 699
EcoT22I ATGCAT 2 cut(s) 199, 934
EcoT38I GRGCYC 2 cut(s) 1074, 3058
ErhI CCWWGG 1 cut(s) 699
Esp3I CGTCTC 1 cut(s) 2585
FalI AAGNNNNNCTT 4 cut(s) 867, 899, 3147, 3179
FaqI GGGAC 4 cut(s) 1028, 1771, 3165, 3238
FauI CCCGC 2 cut(s) 1255, 1548
FauNDI CATATG 1 cut(s) 1618
FblI GTMKAC 1 cut(s) 2165
FriOI GRGCYC 2 cut(s) 1074, 3058
FspBI CTAG 4 cut(s) 215, 1038, 1397, 2499
GlaI GCGC 2 cut(s) 1117, 2466
GsuI CTGGAG 8 cut(s) 333, 411, 2265, 2641, 2667, 2681, 2781, 2966
HaeIII GGCC 8 cut(s) 513, 1095, 1223, 1938, 2093, 2107, 2668, 3056
HapII CCGG 2 cut(s) 2443, 3286
HgaI GACGC 1 cut(s) 291
HhaI GCGC 2 cut(s) 1118, 2467
Hin6I GCGC 2 cut(s) 1116, 2465
HinP1I GCGC 2 cut(s) 1116, 2465
HincII GTYRAC 2 cut(s) 561, 1152
HindII GTYRAC 2 cut(s) 561, 1152
HindIII AAGCTT 2 cut(s) 2744, 3002
HpaII CCGG 2 cut(s) 2443, 3286
HphI GGTGA 4 cut(s) 694, 1101, 1469, 1586
Hpy99I CGWCG 2 cut(s) 307, 2815
HpyCH4III ACNGT 4 cut(s) 336, 1156, 1494, 2543
HpyCH4IV ACGT 1 cut(s) 2578
HpyF10VI GCNNNNNNNGC 8 cut(s) 1247, 1256, 1526, 1662, 2090, 2291, 3010, 3247
HpySE526I ACGT 1 cut(s) 2578
HspAI GCGC 2 cut(s) 1116, 2465
KflI GGGWCCC 1 cut(s) 1042
LmnI GCTCC 5 cut(s) 24, 419, 959, 1069, 1503
LweI GCATC 9 cut(s) 193, 470, 652, 2111, 2206, 2230, 2251, 2452, 3190
MaeI CTAG 4 cut(s) 215, 1038, 1397, 2499
MaeII ACGT 1 cut(s) 2578
MaeIII GTNAC 6 cut(s) 725, 1107, 1183, 1563, 2897, 3043
MbiI CCGCTC 1 cut(s) 1250
MfeI CAATTG 3 cut(s) 1530, 2196, 2967
MflI RGATCY 3 cut(s) 1360, 2038, 2686
MhlI GDGCHC 4 cut(s) 424, 1074, 2133, 3058
MlsI TGGCCA 1 cut(s) 2093
MluNI TGGCCA 1 cut(s) 2093
MlyI GAGTC 3 cut(s) 388, 1753, 1802
MmeI TCCRAC 1 cut(s) 843
Mox20I TGGCCA 1 cut(s) 2093
Mph1103I ATGCAT 2 cut(s) 199, 934
MroXI GAANNNNTTC 3 cut(s) 792, 878, 1430
MscI TGGCCA 1 cut(s) 2093
MslI CAYNNNNRTG 2 cut(s) 287, 487
Msp20I TGGCCA 1 cut(s) 2093
MspI CCGG 2 cut(s) 2443, 3286
MspR9I CCNGG 9 cut(s) 34, 132, 803, 1481, 1771, 2192, 2444, 3258, 3286
MunI CAATTG 3 cut(s) 1530, 2196, 2967
Mva1269I GAATGC 1 cut(s) 197
MvaI CCWGG 7 cut(s) 34, 132, 803, 1481, 1771, 2192, 3258
MvnI CGCG 2 cut(s) 1116, 2224
MwoI GCNNNNNNNGC 8 cut(s) 1247, 1256, 1526, 1662, 2090, 2291, 3010, 3247
NciI CCSGG 2 cut(s) 2444, 3286
NcoI CCATGG 1 cut(s) 699
NdeI CATATG 1 cut(s) 1618
NheI GCTAGC 1 cut(s) 1396
NlaIV GGNNCC 9 cut(s) 250, 961, 1043, 1044, 1626, 1870, 2793, 3055, 3056
NmuCI GTSAC 3 cut(s) 1107, 1183, 1563
NsiI ATGCAT 2 cut(s) 199, 934
NspI RCATGY 4 cut(s) 197, 292, 1864, 2455
NspV TTCGAA 2 cut(s) 70, 1464
PacI TTAATTAA 1 cut(s) 3123
PaeI GCATGC 1 cut(s) 292
PaeR7I CTCGAG 1 cut(s) 50
PagI TCATGA 2 cut(s) 769, 1678
PaqCI CACCTGC 1 cut(s) 670
PasI CCCWGGG 1 cut(s) 1770
PciI ACATGT 2 cut(s) 1860, 2451
PctI GAATGC 1 cut(s) 197
PdmI GAANNNNTTC 3 cut(s) 792, 878, 1430
PflFI GACNNNGTC 1 cut(s) 2390
PflMI CCANNNNNTGG 1 cut(s) 749
PfoI TCCNGGA 1 cut(s) 1479
PleI GAGTC 3 cut(s) 387, 1753, 1801
PpsI GAGTC 3 cut(s) 387, 1753, 1801
PpuMI RGGWCCY 4 cut(s) 1042, 2366, 2792, 3097
PscI ACATGT 2 cut(s) 1860, 2451
PshBI ATTAAT 1 cut(s) 3233
Psp124BI GAGCTC 1 cut(s) 1074
Psp5II RGGWCCY 4 cut(s) 1042, 2366, 2792, 3097
Psp6I CCWGG 7 cut(s) 32, 130, 801, 1479, 1769, 2190, 3256
PspGI CCWGG 7 cut(s) 32, 130, 801, 1479, 1769, 2190, 3256
PspN4I GGNNCC 9 cut(s) 250, 961, 1043, 1044, 1626, 1870, 2793, 3055, 3056
PspOMI GGGCCC 1 cut(s) 3054
PspPI GGNCC 8 cut(s) 1042, 1937, 2181, 2366, 2792, 3054, 3055, 3097
PspPPI RGGWCCY 4 cut(s) 1042, 2366, 2792, 3097
PstI CTGCAG 3 cut(s) 229, 892, 2347
PsuI RGATCY 3 cut(s) 1360, 2038, 2686
PsyI GACNNNGTC 1 cut(s) 2390
RsaI GTAC 6 cut(s) 167, 987, 1174, 1610, 2510, 3190
RsaNI GTAC 6 cut(s) 166, 986, 1173, 1609, 2509, 3189
RseI CAYNNNNRTG 2 cut(s) 287, 487
SacI GAGCTC 1 cut(s) 1074
Sau96I GGNCC 8 cut(s) 1042, 1937, 2181, 2366, 2792, 3054, 3055, 3097
SchI GAGTC 3 cut(s) 388, 1753, 1802
ScrFI CCNGG 9 cut(s) 34, 132, 803, 1481, 1771, 2192, 2444, 3258, 3286
SduI GDGCHC 4 cut(s) 424, 1074, 2133, 3058
SfaNI GCATC 9 cut(s) 193, 470, 652, 2111, 2206, 2230, 2251, 2452, 3190
SfcI CTRYAG 5 cut(s) 225, 639, 888, 2166, 2343
Sfr274I CTCGAG 1 cut(s) 50
SfuI TTCGAA 2 cut(s) 70, 1464
SinI GGWCC 5 cut(s) 1042, 2181, 2366, 2792, 3097
SlaI CTCGAG 1 cut(s) 50
SmiMI CAYNNNNRTG 2 cut(s) 287, 487
SmlI CTYRAG 3 cut(s) 50, 593, 2468
SmoI CTYRAG 3 cut(s) 50, 593, 2468
SphI GCATGC 1 cut(s) 292
SsiI CCGC 7 cut(s) 5, 625, 1248, 1343, 1555, 3041, 3248
SspI AATATT 2 cut(s) 1318, 2413
SspMI CTAG 4 cut(s) 215, 1038, 1397, 2499
SstI GAGCTC 1 cut(s) 1074
StyD4I CCNGG 9 cut(s) 32, 130, 801, 1479, 1769, 2190, 2442, 3256, 3284
StyI CCWWGG 1 cut(s) 699
TaaI ACNGT 4 cut(s) 336, 1156, 1494, 2543
TaiI ACGT 1 cut(s) 2581
TaqI TCGA 8 cut(s) 51, 70, 452, 1464, 2023, 2810, 2859, 3159
TatI WGTACW 1 cut(s) 2508
TauI GCSGC 1 cut(s) 8
TscAI CASTG 6 cut(s) 284, 289, 588, 903, 1441, 2108
TseFI GTSAC 3 cut(s) 1107, 1183, 1563
Tsp45I GTSAC 3 cut(s) 1107, 1183, 1563
TspGWI ACGGA 3 cut(s) 153, 713, 2828
TspRI CASTG 6 cut(s) 284, 289, 588, 903, 1441, 2108
Tth111I GACNNNGTC 1 cut(s) 2390
Van91I CCANNNNNTGG 1 cut(s) 749
VpaK11BI GGWCC 5 cut(s) 1042, 2181, 2366, 2792, 3097
VspI ATTAAT 1 cut(s) 3233
XagI CCTNNNNNAGG 1 cut(s) 670
XapI RAATTY 6 cut(s) 536, 1325, 1460, 1669, 2204, 2266
XceI RCATGY 4 cut(s) 197, 292, 1864, 2455
XhoI CTCGAG 1 cut(s) 50
XmiI GTMKAC 1 cut(s) 2165
XmnI GAANNNNTTC 3 cut(s) 792, 878, 1430
XspI CTAG 4 cut(s) 215, 1038, 1397, 2499
Zsp2I ATGCAT 2 cut(s) 199, 934
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.