Rh1DG150200

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000598
Physical Location & Seq
Forward (+)
31455726 .. 31461962
6237 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG150200.1

Sequence Viewer

Length: 3336 bp
ATGAGAGTAAAGTGGACGACAAAGCTCCACTGTCGCAGTTTGTTTAAAAGGTACTTTTTTAGTAGTAGTACTGAGATGGCGGAGGAAGGCGGCAAGAGCTTTGCCAGGAGAGACCATCTCCTAGAGATTGAGGCAAAGGCTCGAAACTGGTGGGAAGACAAACAAGTTTTCAGGGCCCAGTCTCGTGCAGAGCCGCCCGGCCCAGGGGAGAAGTTCTTTGGAAACTTCCCCTTCCCTTATATGAACGGTTTTCTGCATCTCGGACATGCCTTCTCACTGTCAAAGCTGGAGTTTGCTGCAGCCTATCATAGATTGAGAGGCGCAAATGTCCTCTTGCCATTTGCTTTTCACTGTACTGGCATGCCCATCAAAGCCTCTGCCGATAAGCTTGTTAGGGAGATTCAACAGTTTGGAGAACCTCCTGTTTTTCCCAAAAAAGTGGAGAAAGGGAACGAGGAAGTGGAAGTGGAAGCAGAGGATGTCAATGCAGGCGAACCTCCAGCCAAGCATAAGGGAAAGAAGTCGAAGGCTGCATCAAAATCAAGCGGCGAAGTGTACCAATGGGAAATTATGCAGAGCTTTGGCCTCTCCGACAGTGACATATCCAAATTTCAGGACCCTTATAAGTGGTTGACTTATTTTCCCCCATTGGCTGTGGAGGACCTCAAGGCTTTTGGCCTGGGCTGTGACTGGAGACGTTCCTTTATTACTACAGACATGAACCCTTTTTTCGACGGCTTTGTGAGGTGGCAGGTTAGGAAACTGAAATCCATGGGTAAGATTGTCAAAGATCTCCGTTACACGATATACTCTCCGATGGATGGGCAACCCTGTGCAGATCATGATAGGTCCAGTGGTGAAGGAGTTCAGCCCCAAGAATATACTGTCATCAAAATGGAACTGATGGCACCTTTTCCTTCTAAATTGGGAGTGTTGGAGGGGAGGAAAGTGTTCCTTGCTGCAGCAACACTGAGACCTGAGACCATGTATGGACAAACAAATGCATGGGTATTGCCTGATGGTAAGTATGGTGCATATGAAATCAATGAAACAGATGTGTTCATTCTTACACAGAGAGCAGCACGTAATCTTGCTTATCAAAAGTACTCTAGGGTCCCAGAGAAACCTACTTGCTTGGTTGAACTGACTGGTTACGATTTGATTGGCCTTCCATTGAAGTCGCCACTAGCAGTGAATCAGATTATTTATGCTCTTCCTATGTTGACTGTCCTAACAGACAAAGGTACTGGGATTGTGACTAGTGTACCTACTGATTCTCCGGATGACTATATGGCCTTGCATGACTTGAAATCAAAACCTGCTTTTAGGGCAAAATTTGGTGTGAAAGATGAGTGGGTACTGCCCTTTGAGATTATCCCAATCATCGATATTCCAGGATATGGAAATAAGGCTGCAGAAAAGGTCTGTGCAGATCTCAAGATCAAAAGCCAGAATGAGAAAGAGAAGCTAGCAGAAGCCAAGAGGTTGACATACTTGAAAGGATTCAATGAAGGAACACTGATTGTGGGGGAATTTAAGGGAAGGAAAGTCCAGGAGGTTAAACCCTTGATAAGGAGCAAGCTTATAGAGGCGGGGGAGGCAATATCATATAGTGAACCTGAGAAGCGGGTACTATCACGATCAGGTGATGAATGTGTTGTGGCGCTTACAGATCAATGGTACATCACTTATGGGGAAACTGAATGGAAAAAATTGGCTGAGGAGTGCTTGTCCGGCATGAATTTGTATTCTGATGAGACACGGCATGGATTTGAACACACATTGAGCTGGTTGAATCAGTGGGCTTGTTCACGATCTTTTGGTCTTGGGACTCGCATCCCCTGGGATGAAGAATTCTTAGTTGAGTCATTGTCTGATTCAACTATTTACATGGCTTATTACACCATTGCTCACTTCCTACACAATGGAGACATGTATGGTTCCAGCAAATCTGCAATTAAACCTGAACAAATGACTGATGAGGTCTGGGATTTTATTTTCTGTGATGGCCCATATCCTAAATCATCCGACATCTCATCATCAATTCTGAGTGAGATGAAGCAGGAGTTTGAATATTGGTATCCTTTTGATCTTCGGGTGTCTGGCAAGGATCTAATACAAAATCATTTAACCTTCTGCATCTACAACCATACAGCTATTATGTCCAAGAAGCACTGGCCTCGTGGTTTCAGATGCAATGGGCACATAATGCTCAATGCTCAGAAAATGTCCAAGTCCACAGGAAACTTTAGGACAATTCGCCAGGCAATTGTGGAATTTTCAGCTGATGCTACTCGCTTTTCTTTGGCTGATGCTGGAGATGGTGTTGATGATGCAAATTTTGCATTTGACACTGCCAATGCTGCAATCCTGCGGCTCACTAAAGAGATCTCATGGATGGAAGAAGTTTTAGGTGCAAACTCTTCTTTGAGACCAGGACCCCCAACTACTTATGCTGACAGGGTCTTTGCGAATGAGATAAATATTGCTGTGAACAGGACTGAGCAGAATTACAGCGATTACATGTTCCGAGAAGCCCTCAAGACTGGATTTTATGATCTTCAGGCTGCTAGGGATGAGTATCGGTTTTCATGTGGTGCTGGGGGAATGAATCGTGATTTGGTGTGGCGTTTTATGGATGTGCAGACACGTCTTATTACTCCAATCTGCCCACACTACGGAGAATACGTATGGAGGGAACTTTTGAAGAAGGATGGCTTTGTGGTAAACGCAGGATGGCCTTTGGCTGACGCTCCGGACCTAACCCTCCAGAGTGCCAATAAGTATTTGCAGGACTCAATCATTTCAATGAGGAAGCTCCTCCAAAAGCAAGTTTCTAGCTCAAAGAAGGGTAAGAAGGGTACTCCAGTCACAGTGTTGACAGAAGACAACAATCTGACCGGCTTAATCTATGTGAACGAGCACTTTGATGGATGGAAGGCAGAGTGCCTGAAAATCCTCCAAATTAACTTTAACAGAGACAGTCGTACTTTTTCTTCAGACAAGATGATACAAGAAGAGTTGCAAAAAAGTTCTATTGGTCAGTCGAAAGATTTTAGACAAATCCAAAAGCTTTGTATGCCTTTCATGAAGTTCAAGAAGGATGAGGCAATTGCAATTGGGGCTCAGGCCTTGGATTTGAAGCTGCCTTTTGGTGAGATTGAGGTCCTTCAAGAGAACTTGGACTTAATTAAGAGACAACTTGGTCTTCAAGAGGTAGAAATATTGTCTGCTACAAATCCTGATGCACTTTCTAAAGCCGGTTCTCTTGTCAAACTGTTAGAGCAGAATCCTCCAGCACCCGGAAGCCCTACTGCCATCTTCTTGACCTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

1111

Amino Acids

125.99

Weight (kDa)

6.6

Isoelectric Point (pI)

44.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1 PF00133 49 - 131 7.8e-08 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1 PF00133 218 - 780 8.7e-16 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g PF09334 688 - 786 1.4e-07 tRNA synthetases class I (M)
tRNA-synt_1e PF01406 694 - 770 3e-08 tRNA synthetases class I (C) catalytic domain
Anticodon_1 PF08264 820 - 944 7e-13 Anticodon-binding domain of tRNA ligase
RBD_LARS1 PF24810 973 - 1049 1.4e-06 Leucine--tRNA ligase, RagD-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000598)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09620
fragaria_vesca FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_3g34581 FvH4_4g33720 FvH4_4g33720
malus_domestica MD08G1244600.v1.1 MD15G1439400.v1.1
prunus_persica Prupe.1G582000_v2.0.a1 Prupe.2G015100_v2.0.a1 Prupe.2G123100_v2.0.a1
pyrus_communis pycom08g21210 pycom08g21220 pycom15g38730
rosa_chinensis RchiOBHm_Chr1g0336121 RchiOBHm_Chr1g0353811 RchiOBHm_Chr1g0373331 RchiOBHm_Chr4g0443191 RchiOBHm_Chr4g0443201 RchiOBHm_Chr4g0443211
rosa_laevigata RLG00000003122 RLG00000005948 RLG00000026826 RLG00000028251 RLG00000028252 RLG00000028253 RLG00000029377
rosa_multiflora Rmu_co8002498.1_g000001 Rmu_co8262279.1_g000001 Rmu_co8314139.1_g000001 Rmu_co8393711.1_g000001 Rmu_co8419167.1_g000001 Rmu_sc0007968.1_g000004 Rmu_sc0018919.1_g000004 Rmu_sc0027447.1_g000001
rosa_roxburghii Rroxscaffold_4G00284260 Rroxscaffold_4G00301490 Rroxscaffold_4G00315860 Rroxscaffold_5G00383790 Rroxscaffold_5G00383800
rosa_rugosa Rorug01G0123100 Rorug01G0238700 Rorug01G0377100 Rorug01G0377100 Rorug01G0377200 Rorug01G0377300 Rorug04G0344700 Rorug05G0347900.1
rosa_samantha Rh1AG143800 Rh1AG250300 Rh1AG386300 Rh1BG113400 Rh1BG220800 Rh1BG220900 Rh1BG350400 Rh1CG135500 Rh1CG233100 Rh1CG363500 Rh1DG150200 Rh1DG247700 Rh1DG247800 Rh1DG381300 Rh4AG399400 Rh4AG399500 Rh4BG411000 Rh4BG411100 Rh4BG411200 Rh4CG426500 Rh4CG426600 Rh4DG406100 Rh4DG406200 Rh4DG406300
rosa_wichuraiana Rw0G015190 Rw1G021850 Rw1G034870 Rw4G034440 Rw4G034450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 624
AasI GACNNNNNNGTC 1 cut(s) 3204
Acc36I ACCTGC 2 cut(s) 742, 1327
AccB1I GGYRCC 1 cut(s) 907
AccB7I CCANNNNNTGG 1 cut(s) 1400
AccIII TCCGGA 2 cut(s) 1279, 2755
AciI CCGC 7 cut(s) 80, 90, 194, 546, 1592, 1627, 2374
AclWI GGATC 1 cut(s) 2118
AcsI RAATTY 7 cut(s) 608, 1334, 1532, 1741, 1853, 2276, 2338
AcuI CTGAAG 2 cut(s) 2546, 2982
AfiI CCNNNNNNNGG 7 cut(s) 203, 204, 821, 1400, 1572, 2678, 3302
AflIII ACRYGT 3 cut(s) 1932, 2523, 2648
AhlI ACTAGT 1 cut(s) 1259
AjiI CACGTC 1 cut(s) 2651
AjnI CCWGG 9 cut(s) 104, 202, 678, 1393, 1551, 1841, 2262, 2434, 3329
AjuI GAANNNNNNNTTGG 2 cut(s) 2603, 2635
Alw21I GWGCWC 1 cut(s) 2925
AlwI GGATC 1 cut(s) 2118
Aor13HI TCCGGA 2 cut(s) 1279, 2755
ApaI GGGCCC 1 cut(s) 178
ApoI RAATTY 7 cut(s) 608, 1334, 1532, 1741, 1853, 2276, 2338
ArsI GACNNNNNNTTYG 2 cut(s) 2450, 2482
Asp700I GAANNNNTTC 3 cut(s) 864, 950, 1502
AspLEI GCGC 2 cut(s) 323, 1666
AsuC2I CCSGG 2 cut(s) 198, 3303
AsuHPI GGTGA 3 cut(s) 869, 1658, 3167
AsuNHI GCTAGC 1 cut(s) 1468
AvaII GGWCC 7 cut(s) 616, 661, 849, 1114, 2438, 2758, 3166
BaeGI GKGCMC 2 cut(s) 178, 2205
BaeI ACNNNNGTAYC 4 cut(s) 1248, 1281, 2853, 2886
BanI GGYRCC 1 cut(s) 907
BanII GRGCYC 2 cut(s) 178, 3127
BarI GAAGNNNNNNTAC 2 cut(s) 2980, 3012
BauI CACGAG 2 cut(s) 183, 2181
BbsI GAAGAC 3 cut(s) 162, 2892, 3200
Bbv12I GWGCWC 1 cut(s) 2925
BbvCI CCTCAGC 1 cut(s) 1719
BceAI ACGGC 2 cut(s) 751, 1778
BcgI CGANNNNNNTGC 2 cut(s) 2162, 2196
BciT130I CCWGG 9 cut(s) 106, 204, 680, 1395, 1553, 1843, 2264, 2436, 3331
BciVI GTATCC 1 cut(s) 2091
BcnI CCSGG 2 cut(s) 198, 3303
BcuI ACTAGT 1 cut(s) 1259
BfaI CTAG 7 cut(s) 122, 1110, 1187, 1260, 1469, 2571, 2838
BfmI CTRYAG 4 cut(s) 297, 711, 960, 1413
BfoI RGCGCY 1 cut(s) 1667
BfuAI ACCTGC 2 cut(s) 742, 1327
BfuI GTATCC 1 cut(s) 2091
BglII AGATCT 3 cut(s) 790, 1432, 2388
BmcAI AGTACT 2 cut(s) 70, 1106
Bme18I GGWCC 7 cut(s) 616, 661, 849, 1114, 2438, 2758, 3166
BmgBI CACGTC 1 cut(s) 2651
BmiI GGNNCC 7 cut(s) 176, 618, 909, 1115, 1116, 1942, 2440
BmrI ACTGGG 2 cut(s) 172, 1257
BmsI GCATC 9 cut(s) 265, 542, 1845, 2148, 2183, 2278, 2302, 2323, 3235
BmtI GCTAGC 1 cut(s) 1472
BmuI ACTGGG 2 cut(s) 172, 1257
BpiI GAAGAC 3 cut(s) 162, 2892, 3200
BplI GAGNNNNNCTC 4 cut(s) 102, 134, 2523, 2555
BpmI CTGGAG 7 cut(s) 308, 483, 712, 2337, 2753, 2850, 3279
Bpu10I CCTNAGC 2 cut(s) 1719, 3126
BpuEI CTTGAG 3 cut(s) 650, 1421, 2525
BpuMI CCSGG 2 cut(s) 198, 3303
Bsa29I ATCGAT 1 cut(s) 1386
BsaAI YACGTR 2 cut(s) 1085, 2689
BsaBI GATNNNNATC 1 cut(s) 2580
BsaI GGTCTC 4 cut(s) 105, 967, 974, 2425
BsaJI CCNNGG 7 cut(s) 202, 203, 679, 771, 1841, 1842, 3132
BsaWI WCCGGW 2 cut(s) 1279, 2755
BsaXI ACNNNNNCTCC 8 cut(s) 1261, 1291, 1715, 1745, 2310, 2340, 2673, 2703
Bsc4I CCNNNNNNNGG 7 cut(s) 203, 204, 821, 1400, 1572, 2678, 3302
Bse118I RCCGGY 2 cut(s) 2900, 3260
Bse3DI GCAATG 2 cut(s) 1905, 2203
Bse8I GATNNNNATC 1 cut(s) 2580
BseAI TCCGGA 2 cut(s) 1279, 2755
BseBI CCWGG 9 cut(s) 106, 204, 680, 1395, 1553, 1843, 2264, 2436, 3331
BseCI ATCGAT 1 cut(s) 1386
BseDI CCNNGG 7 cut(s) 202, 203, 679, 771, 1841, 1842, 3132
BseJI GATNNNNATC 1 cut(s) 2580
BseLI CCNNNNNNNGG 7 cut(s) 203, 204, 821, 1400, 1572, 2678, 3302
BseMI GCAATG 2 cut(s) 1905, 2203
BseMII CTCAG 9 cut(s) 63, 962, 969, 1611, 1710, 2039, 2234, 2493, 3140
BseRI GAGGAG 2 cut(s) 1736, 2810
BseSI GKGCMC 2 cut(s) 178, 2205
BseYI CCCAGC 1 cut(s) 2600
BsgI GTGCAG 4 cut(s) 207, 855, 1449, 2663
BshNI GGYRCC 1 cut(s) 907
BshVI ATCGAT 1 cut(s) 1386
BsiHKAI GWGCWC 1 cut(s) 2925
BsiSI CCGG 7 cut(s) 198, 1280, 1734, 2756, 2901, 3261, 3303
BslFI GGGAC 2 cut(s) 1100, 1843
BslI CCNNNNNNNGG 7 cut(s) 203, 204, 821, 1400, 1572, 2678, 3302
BsmBI CGTCTC 1 cut(s) 688
BsmFI GGGAC 2 cut(s) 1100, 1843
Bso31I GGTCTC 4 cut(s) 105, 967, 974, 2425
Bsp120I GGGCCC 1 cut(s) 174
Bsp1286I GDGCHC 4 cut(s) 178, 2205, 2925, 3127
Bsp13I TCCGGA 2 cut(s) 1279, 2755
Bsp19I CCATGG 1 cut(s) 771
BspACI CCGC 7 cut(s) 80, 90, 194, 546, 1592, 1627, 2374
BspCNI CTCAG 9 cut(s) 64, 963, 970, 1612, 1711, 2040, 2233, 2494, 3139
BspDI ATCGAT 1 cut(s) 1386
BspEI TCCGGA 2 cut(s) 1279, 2755
BspHI TCATGA 2 cut(s) 841, 3087
BspLI GGNNCC 7 cut(s) 176, 618, 909, 1115, 1116, 1942, 2440
BspMAI CTGCAG 3 cut(s) 301, 964, 1417
BspMI ACCTGC 2 cut(s) 742, 1327
BspOI GCTAGC 1 cut(s) 1472
BspPI GGATC 1 cut(s) 2118
BspQI GCTCTTC 1 cut(s) 1218
BspT107I GGYRCC 1 cut(s) 907
BspTNI GGTCTC 4 cut(s) 105, 967, 974, 2425
BsrDI GCAATG 2 cut(s) 1905, 2203
BsrFI RCCGGY 2 cut(s) 2900, 3260
BssAI RCCGGY 2 cut(s) 2900, 3260
BssECI CCNNGG 7 cut(s) 202, 203, 679, 771, 1841, 1842, 3132
BssSI CACGAG 2 cut(s) 183, 2181
BssT1I CCWWGG 2 cut(s) 771, 3132
Bst2BI CACGAG 2 cut(s) 183, 2181
Bst2UI CCWGG 9 cut(s) 106, 204, 680, 1395, 1553, 1843, 2264, 2436, 3331
Bst6I CTCTTC 3 cut(s) 1218, 2428, 3012
BstAPI GCANNNNNTGC 2 cut(s) 2209, 2342
BstBAI YACGTR 2 cut(s) 1085, 2689
BstC8I GCNNGC 4 cut(s) 362, 490, 1470, 1580
BstDSI CCRYGG 1 cut(s) 771
BstENI CCTNNNNNAGG 1 cut(s) 1570
BstH2I RGCGCY 1 cut(s) 1667
BstHHI GCGC 2 cut(s) 323, 1666
BstMWI GCNNNNNNNGC 9 cut(s) 96, 1328, 1598, 1734, 2209, 2342, 2363, 3079, 3122
BstNI CCWGG 9 cut(s) 106, 204, 680, 1395, 1553, 1843, 2264, 2436, 3331
BstNSI RCATGY 4 cut(s) 269, 364, 1936, 2527
BstSFI CTRYAG 4 cut(s) 297, 711, 960, 1413
BstSLI GKGCMC 2 cut(s) 178, 2205
BstSNI TACGTA 1 cut(s) 2689
BstV2I GAAGAC 3 cut(s) 162, 2892, 3200
BstX2I RGATCY 4 cut(s) 790, 1432, 2110, 2388
BstXI CCANNNNNNTGG 1 cut(s) 439
BstYI RGATCY 4 cut(s) 790, 1432, 2110, 2388
Bsu15I ATCGAT 1 cut(s) 1386
BsuI GTATCC 1 cut(s) 2091
BsuTUI ATCGAT 1 cut(s) 1386
BtgI CCRYGG 1 cut(s) 771
BtrI CACGTC 1 cut(s) 2651
BtsI GCAGTG 2 cut(s) 1197, 2352
BveI ACCTGC 2 cut(s) 742, 1327
Cac8I GCNNGC 4 cut(s) 362, 490, 1470, 1580
CciI TCATGA 2 cut(s) 841, 3087
CfoI GCGC 2 cut(s) 323, 1666
Cfr10I RCCGGY 2 cut(s) 2900, 3260
ClaI ATCGAT 1 cut(s) 1386
CseI GACGC 1 cut(s) 2759
CsiI ACCWGGT 1 cut(s) 3329
DraI TTTAAA 1 cut(s) 46
DrdI GACNNNNNNGTC 1 cut(s) 3204
DseDI GACNNNNNNGTC 1 cut(s) 3204
Eam1104I CTCTTC 3 cut(s) 1218, 2428, 3012
EarI CTCTTC 3 cut(s) 1218, 2428, 3012
EciI GGCGGA 1 cut(s) 95
Eco105I TACGTA 1 cut(s) 2689
Eco130I CCWWGG 2 cut(s) 771, 3132
Eco147I AGGCCT 1 cut(s) 3131
Eco24I GRGCYC 2 cut(s) 178, 3127
Eco31I GGTCTC 4 cut(s) 105, 967, 974, 2425
Eco47I GGWCC 7 cut(s) 616, 661, 849, 1114, 2438, 2758, 3166
Eco57I CTGAAG 2 cut(s) 2546, 2982
EcoNI CCTNNNNNAGG 1 cut(s) 1570
EcoO109I RGGNCCY 6 cut(s) 174, 616, 661, 1114, 2438, 3166
EcoRI GAATTC 1 cut(s) 1853
EcoRII CCWGG 9 cut(s) 104, 202, 678, 1393, 1551, 1841, 2262, 2434, 3329
EcoT14I CCWWGG 2 cut(s) 771, 3132
EcoT22I ATGCAT 1 cut(s) 1006
EcoT38I GRGCYC 2 cut(s) 178, 3127
ErhI CCWWGG 2 cut(s) 771, 3132
Esp3I CGTCTC 1 cut(s) 688
FalI AAGNNNNNCTT 4 cut(s) 939, 971, 2702, 2734
FaqI GGGAC 2 cut(s) 1100, 1843
FauI CCCGC 2 cut(s) 1585, 1620
FauNDI CATATG 1 cut(s) 1036
FriOI GRGCYC 2 cut(s) 178, 3127
FspBI CTAG 7 cut(s) 122, 1110, 1187, 1260, 1469, 2571, 2838
GlaI GCGC 2 cut(s) 322, 1665
GsaI CCCAGC 1 cut(s) 2604
GsuI CTGGAG 7 cut(s) 308, 483, 712, 2337, 2753, 2850, 3279
HaeII RGCGCY 1 cut(s) 1667
HapII CCGG 7 cut(s) 198, 1280, 1734, 2756, 2901, 3261, 3303
HgaI GACGC 1 cut(s) 2759
HhaI GCGC 2 cut(s) 323, 1666
Hin6I GCGC 2 cut(s) 321, 1664
HinP1I GCGC 2 cut(s) 321, 1664
HincII GTYRAC 4 cut(s) 633, 1224, 1488, 2880
HindII GTYRAC 4 cut(s) 633, 1224, 1488, 2880
HindIII AAGCTT 3 cut(s) 386, 1580, 3071
HpaII CCGG 7 cut(s) 198, 1280, 1734, 2756, 2901, 3261, 3303
HphI GGTGA 3 cut(s) 869, 1658, 3167
Hpy99I CGWCG 1 cut(s) 737
HpyCH4IV ACGT 4 cut(s) 697, 1084, 2650, 2688
HpyF10VI GCNNNNNNNGC 9 cut(s) 96, 1328, 1598, 1734, 2209, 2342, 2363, 3079, 3122
HpySE526I ACGT 4 cut(s) 697, 1084, 2650, 2688
HspAI GCGC 2 cut(s) 321, 1664
KflI GGGWCCC 1 cut(s) 1114
Kpn2I TCCGGA 2 cut(s) 1279, 2755
LguI GCTCTTC 1 cut(s) 1218
LmnI GCTCC 4 cut(s) 30, 1575, 2758, 2823
LweI GCATC 9 cut(s) 265, 542, 1845, 2148, 2183, 2278, 2302, 2323, 3235
MabI ACCWGGT 1 cut(s) 3329
MaeI CTAG 7 cut(s) 122, 1110, 1187, 1260, 1469, 2571, 2838
MaeII ACGT 4 cut(s) 697, 1084, 2650, 2688
MaeIII GTNAC 6 cut(s) 596, 686, 797, 1151, 1255, 2869
MfeI CAATTG 3 cut(s) 2268, 3111, 3117
MflI RGATCY 4 cut(s) 790, 1432, 2110, 2388
MhlI GDGCHC 4 cut(s) 178, 2205, 2925, 3127
MlyI GAGTC 3 cut(s) 1825, 1874, 2789
MmeI TCCRAC 3 cut(s) 615, 915, 2052
Mph1103I ATGCAT 1 cut(s) 1006
MroI TCCGGA 2 cut(s) 1279, 2755
MroXI GAANNNNTTC 3 cut(s) 864, 950, 1502
MslI CAYNNNNRTG 3 cut(s) 893, 2807, 2928
MspA1I CMGCKG 1 cut(s) 2285
MspI CCGG 7 cut(s) 198, 1280, 1734, 2756, 2901, 3261, 3303
MunI CAATTG 3 cut(s) 2268, 3111, 3117
MvaI CCWGG 9 cut(s) 106, 204, 680, 1395, 1553, 1843, 2264, 2436, 3331
MwoI GCNNNNNNNGC 9 cut(s) 96, 1328, 1598, 1734, 2209, 2342, 2363, 3079, 3122
NciI CCSGG 2 cut(s) 198, 3303
NcoI CCATGG 1 cut(s) 771
NdeI CATATG 1 cut(s) 1036
NheI GCTAGC 1 cut(s) 1468
NlaIV GGNNCC 7 cut(s) 176, 618, 909, 1115, 1116, 1942, 2440
NmuCI GTSAC 4 cut(s) 596, 686, 1255, 2869
NsiI ATGCAT 1 cut(s) 1006
NspI RCATGY 4 cut(s) 269, 364, 1936, 2527
PacI TTAATTAA 1 cut(s) 3192
PaeI GCATGC 1 cut(s) 364
PagI TCATGA 2 cut(s) 841, 3087
PasI CCCWGGG 2 cut(s) 203, 1842
PceI AGGCCT 1 cut(s) 3131
PciI ACATGT 2 cut(s) 1932, 2523
PciSI GCTCTTC 1 cut(s) 1218
PcsI WCGNNNNNNNCGW 1 cut(s) 2685
PdmI GAANNNNTTC 3 cut(s) 864, 950, 1502
PfeI GAWTC 8 cut(s) 400, 1195, 1274, 1503, 1795, 1877, 2611, 3289
PflFI GACNNNGTC 1 cut(s) 2462
PflMI CCANNNNNTGG 1 cut(s) 1400
PfoI TCCNGGA 2 cut(s) 1393, 1551
PleI GAGTC 3 cut(s) 1825, 1873, 2789
PpsI GAGTC 3 cut(s) 1825, 1873, 2789
Ppu21I YACGTR 2 cut(s) 1085, 2689
PpuMI RGGWCCY 5 cut(s) 616, 661, 1114, 2438, 3166
PscI ACATGT 2 cut(s) 1932, 2523
PsiI TTATAA 1 cut(s) 624
Psp5II RGGWCCY 5 cut(s) 616, 661, 1114, 2438, 3166
Psp6I CCWGG 9 cut(s) 104, 202, 678, 1393, 1551, 1841, 2262, 2434, 3329
PspFI CCCAGC 1 cut(s) 2600
PspGI CCWGG 9 cut(s) 104, 202, 678, 1393, 1551, 1841, 2262, 2434, 3329
PspN4I GGNNCC 7 cut(s) 176, 618, 909, 1115, 1116, 1942, 2440
PspOMI GGGCCC 1 cut(s) 174
PspPPI RGGWCCY 5 cut(s) 616, 661, 1114, 2438, 3166
PstI CTGCAG 3 cut(s) 301, 964, 1417
PsuI RGATCY 4 cut(s) 790, 1432, 2110, 2388
PsyI GACNNNGTC 1 cut(s) 2462
PvuII CAGCTG 1 cut(s) 2285
RseI CAYNNNNRTG 3 cut(s) 893, 2807, 2928
SapI GCTCTTC 1 cut(s) 1218
ScaI AGTACT 2 cut(s) 70, 1106
SchI GAGTC 3 cut(s) 1825, 1874, 2789
SduI GDGCHC 4 cut(s) 178, 2205, 2925, 3127
SexAI ACCWGGT 1 cut(s) 3329
SfaNI GCATC 9 cut(s) 265, 542, 1845, 2148, 2183, 2278, 2302, 2323, 3235
SfcI CTRYAG 4 cut(s) 297, 711, 960, 1413
SinI GGWCC 7 cut(s) 616, 661, 849, 1114, 2438, 2758, 3166
SmiMI CAYNNNNRTG 3 cut(s) 893, 2807, 2928
SmlI CTYRAG 3 cut(s) 665, 1436, 2540
SmoI CTYRAG 3 cut(s) 665, 1436, 2540
SnaBI TACGTA 1 cut(s) 2689
SpeI ACTAGT 1 cut(s) 1259
SphI GCATGC 1 cut(s) 364
SseBI AGGCCT 1 cut(s) 3131
SsiI CCGC 7 cut(s) 80, 90, 194, 546, 1592, 1627, 2374
SspI AATATT 3 cut(s) 2075, 2485, 3225
SspMI CTAG 7 cut(s) 122, 1110, 1187, 1260, 1469, 2571, 2838
StuI AGGCCT 1 cut(s) 3131
StyI CCWWGG 2 cut(s) 771, 3132
TaiI ACGT 4 cut(s) 700, 1087, 2653, 2691
TaqI TCGA 5 cut(s) 142, 524, 732, 1386, 3047
TatI WGTACW 3 cut(s) 68, 353, 1104
TauI GCSGC 4 cut(s) 93, 196, 549, 2377
TfiI GAWTC 8 cut(s) 400, 1195, 1274, 1503, 1795, 1877, 2611, 3289
TseFI GTSAC 4 cut(s) 596, 686, 1255, 2869
Tsp45I GTSAC 4 cut(s) 596, 686, 1255, 2869
TspGWI ACGGA 2 cut(s) 785, 2694
Tth111I GACNNNGTC 1 cut(s) 2462
Van91I CCANNNNNTGG 1 cut(s) 1400
VpaK11BI GGWCC 7 cut(s) 616, 661, 849, 1114, 2438, 2758, 3166
XagI CCTNNNNNAGG 1 cut(s) 1570
XapI RAATTY 7 cut(s) 608, 1334, 1532, 1741, 1853, 2276, 2338
XceI RCATGY 4 cut(s) 269, 364, 1936, 2527
XmnI GAANNNNTTC 3 cut(s) 864, 950, 1502
XspI CTAG 7 cut(s) 122, 1110, 1187, 1260, 1469, 2571, 2838
ZrmI AGTACT 2 cut(s) 70, 1106
Zsp2I ATGCAT 1 cut(s) 1006
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.