pycom08g21210

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000598
Physical Location & Seq
Forward (+)
20760441 .. 20763690
3250 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g21210.2

Sequence Viewer

Length: 2313 bp
ATGATTCATGATATGAAAATTTCTCTCACAACAAGAAGTCCGAGTCCCCAAAACCCTAACTTACGCCTCACCTTCTTCTTCTCAGCTGCTTGCTACCTTCTCTCCCTCCCTCCCTCTTCTGCTAATATCACGTCGTTTTCTTCTCAGAGGAAGAAGGAAACCCTAATCTCCCTCCTGGCTTTTCTTCAAGCTCGAGAAAGCGCGCGCAAACTAACCTATAATTCCCTTCCCTTTTCAATCTTGAACCGCAGTTTCTGTAAGATTACTGAAATGGCGGCTGAAGGTGGTGGGAAGAGCTTTGCCAGGAGAGATCACCTTCTCGAGATTGAAACAAAGGTTCGAAGCTGGTGGGAAGAGAAGGATGTTTTCAGGGCTGAATCATGTGAAAAGCTCCCTGAACCTGGGGAGAAGTTCTTTGGAAACTTCCCGTTTCCTTACATGAATGGCTTTTTGCATCTTGGACATGCATTCTCACTCTCCAAGCTAGAGTTTGCAGCAGCTTACCATAGGTTAAGAGGTGCCAATGTGCTTTTGCCTTTTGGTTTTCACTGCACTGGCATGCCTATTAAAGCGTCTGCTGATAAACTTGCGAGAGAGATTGAACTATTTGGCAACCCGCCGGTTTTTCCAAAAGAAGTAGAACAGGGGAGTCAGGAAGTAGAAGCAGAAGATGCAAATAATGGAGCACCTCCCGACAAGTTCAAGGGAAAGAAGTCGAAGGCTGCATCAAAATCAGGTGGACAAGTATACCAATGGGAAATCATGCGTAGTTTTGGCCTCTCAGATAGCGAGATATCCAAATTCCAAGATCCATACAATTGGTTGACATTCTTCCCTCCATTGGCAGTGGAAGACCTCAAGGCTTTTGGATTGGGATGTGACTGGAGACGGTCATTTATTACTACAGACATGAACCCATTTTTTGATGCCTTTGTGAGGTGGCAGATGAGGAAACTAAAATCTATGGGCAAGATTGTGAAAGACGTCCGTTATGCAATTTACTCTCCACTGGATGGGCAGCCCTGTGCAGATCATGACAGGGCAAGTGGCGAAGGAGTTCAGCCCCAGGAATACACCCTCATCAAAATGGAGGTGGTGGCACCTTTTCCATCTAAACTGAAAGTGTTGGAGGGAAGGAAAGTGTTCCTTGCTGCAGCGACACTTAGGCCTGAGACCATGTATGGACAGACAAATGCATGGGTATTGCCTGATGGGAAATATGGAGCCTTCGAAATCAACGAAACAGAGGTATTTATTGTAACGCAAAGAGCAGCACTTAATCTTGCCTATCAGAAGTATTCTAGGGTTCCAGAGAAACCTACTTGCTTAGTGGAGCTCACGGGTCATGATTTGATTGGCCTTCCATTGAAGTCACCACGCACAATCAATCAGATCATTTACACTCTTCCTATGTTGACTGTCCTGACAGACAAAGGTACTGGGATTGTGACCAGTGTGCCTGCTGATTCTCCTGATGATTATATGGCCTTGCATGATTTAAAAGCAAAACCTGCTCTTAGAGCAAAATATGGCGTGAAGGATGAGTGGGTCATGCCCTTTGAGATTATTCCAATTATCAATATTCCTGATTTCGGGAATAAGGCTGCAGAAAAGGTCTGTGCAGATCTAAAAATCAAGAGCCAGAATGAGAAAGAGAAGCTAGCAGAAGCCAAGAGGCTGACATACCTGAAAGGATTCACTGAAGGAACACTTATCGTGGGTGAATTTGAAGGAAGGAAGGTCCAGGAGGCGAAACCGTTGATAAGGAGCAAGCTCATAGAGTCAAATGAGGCAATTGTATATAGTGAACCTGAGAAGCGGGTGGTGTCACGATCAGCTGATGAGTGTGTTGTTGCTCTCACAGATCAATGGTACATCACATATGGGGAACCTGAATGGAAAAAACTGGCGGAGGAGTGCTTGTCCAGCATGAATTTGTATCATGACGAGACAAAACATGGATTTGAACACACATTGGATTGGTTGAATCAGTGGGCTTGTTCACGATCTTTTGGGCTTGGGACTCGTATTCCCTGGGATGAAGAGTTCTTAGTTGAGTCGTTGTCTGATTCAACTATTTACATGGCTTATTACACTATCGCTCACTTTTTACACAATGGAGACATGTATGGTCCCAGCAAATCTGCAATTCAACCTGAACAAATGACTGATGAGGTCTGGGAGTATATTTTCTGTGATGGCCCATATCCTGAATCATCTAACATATCGTCATCCATCCTTAATAAGATGAAGCAGGAGTTTGAGTATTGGTATCCTTTTGATCTTCGAGTGTCTGGCAAGCGATCGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

771

Amino Acids

87.38

Weight (kDa)

6.24

Isoelectric Point (pI)

49.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000598)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09620
fragaria_vesca FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_3g34581 FvH4_4g33720 FvH4_4g33720
malus_domestica MD08G1244600.v1.1 MD15G1439400.v1.1
prunus_persica Prupe.1G582000_v2.0.a1 Prupe.2G015100_v2.0.a1 Prupe.2G123100_v2.0.a1
pyrus_communis pycom08g21210 pycom08g21220 pycom15g38730
rosa_chinensis RchiOBHm_Chr1g0336121 RchiOBHm_Chr1g0353811 RchiOBHm_Chr1g0373331 RchiOBHm_Chr4g0443191 RchiOBHm_Chr4g0443201 RchiOBHm_Chr4g0443211
rosa_laevigata RLG00000003122 RLG00000005948 RLG00000026826 RLG00000028251 RLG00000028252 RLG00000028253 RLG00000029377
rosa_multiflora Rmu_co8002498.1_g000001 Rmu_co8262279.1_g000001 Rmu_co8314139.1_g000001 Rmu_co8393711.1_g000001 Rmu_co8419167.1_g000001 Rmu_sc0007968.1_g000004 Rmu_sc0018919.1_g000004 Rmu_sc0027447.1_g000001
rosa_roxburghii Rroxscaffold_4G00284260 Rroxscaffold_4G00301490 Rroxscaffold_4G00315860 Rroxscaffold_5G00383790 Rroxscaffold_5G00383800
rosa_rugosa Rorug01G0123100 Rorug01G0238700 Rorug01G0377100 Rorug01G0377100 Rorug01G0377200 Rorug01G0377300 Rorug04G0344700 Rorug05G0347900.1
rosa_samantha Rh1AG143800 Rh1AG250300 Rh1AG386300 Rh1BG113400 Rh1BG220800 Rh1BG220900 Rh1BG350400 Rh1CG135500 Rh1CG233100 Rh1CG363500 Rh1DG150200 Rh1DG247700 Rh1DG247800 Rh1DG381300 Rh4AG399400 Rh4AG399500 Rh4BG411000 Rh4BG411100 Rh4BG411200 Rh4CG426500 Rh4CG426600 Rh4DG406100 Rh4DG406200 Rh4DG406300
rosa_wichuraiana Rw0G015190 Rw1G021850 Rw1G034870 Rw4G034440 Rw4G034450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 987
Acc36I ACCTGC 1 cut(s) 1519
AccB1I GGYRCC 2 cut(s) 518, 1099
AccB7I CCANNNNNTGG 1 cut(s) 1013
AccI GTMKAC 1 cut(s) 747
AccII CGCG 2 cut(s) 203, 205
AciI CCGC 5 cut(s) 247, 275, 617, 1819, 1910
AclWI GGATC 1 cut(s) 803
AcsI RAATTY 4 cut(s) 18, 800, 1724, 1933
AcuI CTGAAG 2 cut(s) 300, 1722
AcyI GRCGYC 1 cut(s) 984
AfaI GTAC 2 cut(s) 1438, 1874
AfiI CCNNNNNNNGG 5 cut(s) 401, 841, 936, 1013, 1592
AflIII ACRYGT 1 cut(s) 2124
AjiI CACGTC 1 cut(s) 132
AjnI CCWGG 6 cut(s) 174, 302, 400, 1065, 1743, 2033
AloI GAACNNNNNNTCC 2 cut(s) 2030, 2062
Alw21I GWGCWC 2 cut(s) 688, 1338
Alw26I GTCTC 4 cut(s) 880, 1166, 1943, 2115
AlwI GGATC 1 cut(s) 803
AlwNI CAGNNNCTG 1 cut(s) 255
Ama87I CYCGRG 2 cut(s) 192, 320
AoxI GGCC 5 cut(s) 775, 1166, 1357, 1485, 2200
ApeKI GCWGC 9 cut(s) 86, 494, 497, 722, 1018, 1151, 1154, 1271, 1604
ApoI RAATTY 4 cut(s) 18, 800, 1724, 1933
Asp700I GAANNNNTTC 3 cut(s) 1056, 1142, 1694
AspLEI GCGC 3 cut(s) 203, 205, 207
AspS9I GGNCC 3 cut(s) 1741, 2132, 2201
AsuHPI GGTGA 4 cut(s) 61, 305, 1365, 1733
AsuII TTCGAA 2 cut(s) 340, 1230
AsuNHI GCTAGC 1 cut(s) 1660
AvaI CYCGRG 2 cut(s) 192, 320
AvaII GGWCC 2 cut(s) 1741, 2132
BanI GGYRCC 2 cut(s) 518, 1099
BanII GRGCYC 1 cut(s) 1338
BbsI GAAGAC 1 cut(s) 858
Bbv12I GWGCWC 2 cut(s) 688, 1338
BbvI GCAGC 9 cut(s) 73, 506, 509, 709, 1030, 1138, 1166, 1283, 1591
BccI CCATC 5 cut(s) 1007, 1117, 1205, 2192, 2243
BciT130I CCWGG 6 cut(s) 176, 304, 402, 1067, 1745, 2035
BciVI GTATCC 1 cut(s) 2283
BcoDI GTCTC 4 cut(s) 880, 1166, 1943, 2115
BfaI CTAG 3 cut(s) 485, 1302, 1661
BfmI CTRYAG 3 cut(s) 903, 1152, 1605
BfuAI ACCTGC 1 cut(s) 1519
BfuI GTATCC 1 cut(s) 2283
BglII AGATCT 1 cut(s) 1624
Bme1390I CCNGG 6 cut(s) 176, 304, 402, 1067, 1745, 2035
Bme18I GGWCC 2 cut(s) 1741, 2132
BmeT110I CYCGRG 2 cut(s) 192, 320
BmgBI CACGTC 1 cut(s) 132
BmgT120I GGNCC 3 cut(s) 1741, 2132, 2201
BmiI GGNNCC 6 cut(s) 520, 1101, 1225, 1308, 1890, 2134
BmrFI CCNGG 6 cut(s) 176, 304, 402, 1067, 1745, 2035
BmrI ACTGGG 1 cut(s) 1449
BmsI GCATC 4 cut(s) 463, 661, 734, 916
BmtI GCTAGC 1 cut(s) 1664
BmuI ACTGGG 1 cut(s) 1449
BpiI GAAGAC 1 cut(s) 858
BpmI CTGGAG 1 cut(s) 904
Bpu14I TTCGAA 2 cut(s) 340, 1230
BpuEI CTTGAG 1 cut(s) 842
Bsa29I ATCGAT 1 cut(s) 2306
BsaHI GRCGYC 1 cut(s) 984
BsaI GGTCTC 1 cut(s) 1166
BsaJI CCNNGG 4 cut(s) 401, 1065, 2033, 2034
BsaXI ACNNNNNCTCC 6 cut(s) 86, 116, 1907, 1937, 2249, 2279
Bsc4I CCNNNNNNNGG 5 cut(s) 401, 841, 936, 1013, 1592
Bse118I RCCGGY 1 cut(s) 619
Bse1I ACTGG 6 cut(s) 559, 887, 1014, 1444, 1452, 1911
BseBI CCWGG 6 cut(s) 176, 304, 402, 1067, 1745, 2035
BseCI ATCGAT 1 cut(s) 2306
BseDI CCNNGG 4 cut(s) 401, 1065, 2033, 2034
BseGI GGATG 7 cut(s) 367, 881, 1018, 1546, 2044, 2231, 2235
BseLI CCNNNNNNNGG 5 cut(s) 401, 841, 936, 1013, 1592
BseMII CTCAG 5 cut(s) 96, 158, 795, 1161, 1803
BseNI ACTGG 6 cut(s) 559, 887, 1014, 1444, 1452, 1911
BsePI GCGCGC 2 cut(s) 201, 203
BseRI GAGGAG 1 cut(s) 1928
BseXI GCAGC 9 cut(s) 73, 506, 509, 709, 1030, 1138, 1166, 1283, 1591
BseYI CCCAGC 1 cut(s) 2135
BsgI GTGCAG 3 cut(s) 535, 1047, 1641
Bsh1236I CGCG 2 cut(s) 203, 205
Bsh1285I CGRYCG 1 cut(s) 2306
BshFI GGCC 5 cut(s) 777, 1168, 1359, 1487, 2202
BshNI GGYRCC 2 cut(s) 518, 1099
BshVI ATCGAT 1 cut(s) 2306
BsiEI CGRYCG 1 cut(s) 2306
BsiHKAI GWGCWC 2 cut(s) 688, 1338
BsiHKCI CYCGRG 2 cut(s) 192, 320
BsiSI CCGG 1 cut(s) 620
BslFI GGGAC 3 cut(s) 30, 2035, 2118
BslI CCNNNNNNNGG 5 cut(s) 401, 841, 936, 1013, 1592
BsmAI GTCTC 4 cut(s) 880, 1166, 1943, 2115
BsmBI CGTCTC 1 cut(s) 880
BsmFI GGGAC 3 cut(s) 30, 2035, 2118
BsmI GAATGC 1 cut(s) 467
BsnI GGCC 5 cut(s) 777, 1168, 1359, 1487, 2202
Bso31I GGTCTC 1 cut(s) 1166
BsoBI CYCGRG 2 cut(s) 192, 320
Bsp119I TTCGAA 2 cut(s) 340, 1230
Bsp1286I GDGCHC 2 cut(s) 688, 1338
BspACI CCGC 5 cut(s) 247, 275, 617, 1819, 1910
BspANI GGCC 5 cut(s) 777, 1168, 1359, 1487, 2202
BspCNI CTCAG 5 cut(s) 95, 157, 794, 1162, 1804
BspDI ATCGAT 1 cut(s) 2306
BspFNI CGCG 2 cut(s) 203, 205
BspHI TCATGA 4 cut(s) 7, 1033, 1345, 1942
BspLI GGNNCC 6 cut(s) 520, 1101, 1225, 1308, 1890, 2134
BspMAI CTGCAG 2 cut(s) 1156, 1609
BspMI ACCTGC 1 cut(s) 1519
BspOI GCTAGC 1 cut(s) 1664
BspPI GGATC 1 cut(s) 803
BspQI GCTCTTC 1 cut(s) 287
BspT104I TTCGAA 2 cut(s) 340, 1230
BspT107I GGYRCC 2 cut(s) 518, 1099
BspTNI GGTCTC 1 cut(s) 1166
BsrFI RCCGGY 1 cut(s) 619
BsrI ACTGG 6 cut(s) 559, 887, 1014, 1444, 1452, 1911
BssAI RCCGGY 1 cut(s) 619
BssECI CCNNGG 4 cut(s) 401, 1065, 2033, 2034
BssHII GCGCGC 2 cut(s) 201, 203
BssNAI GTATAC 1 cut(s) 748
BssNI GRCGYC 1 cut(s) 984
Bst1107I GTATAC 1 cut(s) 748
Bst2UI CCWGG 6 cut(s) 176, 304, 402, 1067, 1745, 2035
Bst4CI ACNGT 3 cut(s) 891, 1420, 1758
Bst6I CTCTTC 5 cut(s) 121, 287, 348, 1410, 2037
BstACI GRCGYC 1 cut(s) 984
BstAPI GCANNNNNTGC 2 cut(s) 671, 1511
BstBI TTCGAA 2 cut(s) 340, 1230
BstC8I GCNNGC 8 cut(s) 91, 203, 205, 560, 1461, 1662, 1772, 2300
BstDEI CTNAG 9 cut(s) 82, 144, 781, 1163, 1170, 1327, 1517, 1812, 2050
BstENI CCTNNNNNAGG 1 cut(s) 934
BstF5I GGATG 7 cut(s) 367, 881, 1018, 1546, 2044, 2231, 2235
BstFNI CGCG 2 cut(s) 203, 205
BstHHI GCGC 3 cut(s) 203, 205, 207
BstMAI GTCTC 4 cut(s) 880, 1166, 1943, 2115
BstMCI CGRYCG 1 cut(s) 2306
BstMWI GCNNNNNNNGC 4 cut(s) 671, 1511, 1520, 1926
BstNI CCWGG 6 cut(s) 176, 304, 402, 1067, 1745, 2035
BstNSI RCATGY 3 cut(s) 467, 562, 2128
BstSCI CCNGG 6 cut(s) 174, 302, 400, 1065, 1743, 2033
BstSFI CTRYAG 3 cut(s) 903, 1152, 1605
BstUI CGCG 2 cut(s) 203, 205
BstV1I GCAGC 9 cut(s) 73, 506, 509, 709, 1030, 1138, 1166, 1283, 1591
BstV2I GAAGAC 1 cut(s) 858
BstX2I RGATCY 2 cut(s) 808, 1624
BstXI CCANNNNNNTGG 1 cut(s) 819
BstYI RGATCY 2 cut(s) 808, 1624
BstZ17I GTATAC 1 cut(s) 748
Bsu15I ATCGAT 1 cut(s) 2306
BsuI GTATCC 1 cut(s) 2283
BsuRI GGCC 5 cut(s) 777, 1168, 1359, 1487, 2202
BsuTUI ATCGAT 1 cut(s) 2306
BtrI CACGTC 1 cut(s) 132
BtsCI GGATG 7 cut(s) 367, 881, 1018, 1546, 2044, 2231, 2235
BtsI GCAGTG 2 cut(s) 547, 852
BtsIMutI CAGTG 7 cut(s) 547, 552, 852, 1007, 1459, 1698, 1997
BveI ACCTGC 1 cut(s) 1519
Cac8I GCNNGC 8 cut(s) 91, 203, 205, 560, 1461, 1662, 1772, 2300
CaiI CAGNNNCTG 1 cut(s) 255
CciI TCATGA 4 cut(s) 7, 1033, 1345, 1942
CfoI GCGC 3 cut(s) 203, 205, 207
Cfr10I RCCGGY 1 cut(s) 619
Cfr13I GGNCC 3 cut(s) 1741, 2132, 2201
ClaI ATCGAT 1 cut(s) 2306
CseI GACGC 1 cut(s) 561
Csp6I GTAC 2 cut(s) 1437, 1873
CviQI GTAC 2 cut(s) 1437, 1873
DdeI CTNAG 9 cut(s) 82, 144, 781, 1163, 1170, 1327, 1517, 1812, 2050
DraI TTTAAA 1 cut(s) 1500
Eam1104I CTCTTC 5 cut(s) 121, 287, 348, 1410, 2037
EarI CTCTTC 5 cut(s) 121, 287, 348, 1410, 2037
EciI GGCGGA 1 cut(s) 1925
Ecl136II GAGCTC 1 cut(s) 1336
Eco147I AGGCCT 1 cut(s) 1168
Eco24I GRGCYC 1 cut(s) 1338
Eco31I GGTCTC 1 cut(s) 1166
Eco32I GATATC 1 cut(s) 795
Eco47I GGWCC 2 cut(s) 1741, 2132
Eco53kI GAGCTC 1 cut(s) 1336
Eco57I CTGAAG 2 cut(s) 300, 1722
Eco88I CYCGRG 2 cut(s) 192, 320
EcoICRI GAGCTC 1 cut(s) 1336
EcoNI CCTNNNNNAGG 1 cut(s) 934
EcoRII CCWGG 6 cut(s) 174, 302, 400, 1065, 1743, 2033
EcoRV GATATC 1 cut(s) 795
EcoT22I ATGCAT 2 cut(s) 469, 1198
EcoT38I GRGCYC 1 cut(s) 1338
Esp3I CGTCTC 1 cut(s) 880
FalI AAGNNNNNCTT 4 cut(s) 1131, 1163, 1695, 1727
FaqI GGGAC 3 cut(s) 30, 2035, 2118
FauI CCCGC 2 cut(s) 624, 1812
FauNDI CATATG 1 cut(s) 1882
FblI GTMKAC 1 cut(s) 747
FokI GGATG 7 cut(s) 374, 888, 1025, 1553, 2051, 2218, 2222
FriOI GRGCYC 1 cut(s) 1338
FspBI CTAG 3 cut(s) 485, 1302, 1661
GlaI GCGC 3 cut(s) 202, 204, 206
GsaI CCCAGC 1 cut(s) 2139
GsuI CTGGAG 1 cut(s) 904
HaeIII GGCC 5 cut(s) 777, 1168, 1359, 1487, 2202
HapII CCGG 1 cut(s) 620
HgaI GACGC 1 cut(s) 561
HhaI GCGC 3 cut(s) 203, 205, 207
Hin1I GRCGYC 1 cut(s) 984
Hin6I GCGC 3 cut(s) 201, 203, 205
HinP1I GCGC 3 cut(s) 201, 203, 205
HincII GTYRAC 2 cut(s) 825, 1416
HindII GTYRAC 2 cut(s) 825, 1416
HpaII CCGG 1 cut(s) 620
HphI GGTGA 4 cut(s) 61, 305, 1365, 1733
Hpy166II GTNNAC 6 cut(s) 740, 748, 825, 1416, 1808, 2003
Hpy188I TCNGA 6 cut(s) 42, 147, 784, 1293, 1392, 2068
Hpy8I GTNNAC 6 cut(s) 740, 748, 825, 1416, 1808, 2003
Hpy99I CGWCG 1 cut(s) 136
HpyCH4III ACNGT 3 cut(s) 891, 1420, 1758
HpyCH4IV ACGT 2 cut(s) 131, 984
HpyF10VI GCNNNNNNNGC 4 cut(s) 671, 1511, 1520, 1926
HpyF3I CTNAG 9 cut(s) 82, 144, 781, 1163, 1170, 1327, 1517, 1812, 2050
HpySE526I ACGT 2 cut(s) 131, 984
Hsp92I GRCGYC 1 cut(s) 984
HspAI GCGC 3 cut(s) 201, 203, 205
LguI GCTCTTC 1 cut(s) 287
LmnI GCTCC 5 cut(s) 396, 683, 1223, 1333, 1767
Lsp1109I GCAGC 9 cut(s) 73, 506, 509, 709, 1030, 1138, 1166, 1283, 1591
LweI GCATC 4 cut(s) 463, 661, 734, 916
MaeI CTAG 3 cut(s) 485, 1302, 1661
MaeII ACGT 2 cut(s) 131, 984
MaeIII GTNAC 5 cut(s) 878, 1258, 1371, 1447, 1827
MfeI CAATTG 2 cut(s) 817, 1794
MflI RGATCY 2 cut(s) 808, 1624
MhlI GDGCHC 2 cut(s) 688, 1338
MlyI GAGTC 5 cut(s) 52, 658, 1790, 2017, 2066
MmeI TCCRAC 1 cut(s) 1107
Mph1103I ATGCAT 2 cut(s) 469, 1198
MroXI GAANNNNTTC 3 cut(s) 1056, 1142, 1694
MseI TTAA 5 cut(s) 512, 567, 1278, 1499, 2241
MslI CAYNNNNRTG 2 cut(s) 557, 1085
MspA1I CMGCKG 2 cut(s) 86, 1838
MspI CCGG 1 cut(s) 620
MspR9I CCNGG 6 cut(s) 176, 304, 402, 1067, 1745, 2035
MunI CAATTG 2 cut(s) 817, 1794
Mva1269I GAATGC 1 cut(s) 467
MvaI CCWGG 6 cut(s) 176, 304, 402, 1067, 1745, 2035
MvnI CGCG 2 cut(s) 203, 205
MwoI GCNNNNNNNGC 4 cut(s) 671, 1511, 1520, 1926
NdeI CATATG 1 cut(s) 1882
NheI GCTAGC 1 cut(s) 1660
NlaIV GGNNCC 6 cut(s) 520, 1101, 1225, 1308, 1890, 2134
NmuCI GTSAC 4 cut(s) 878, 1371, 1447, 1827
NsiI ATGCAT 2 cut(s) 469, 1198
NspI RCATGY 3 cut(s) 467, 562, 2128
NspV TTCGAA 2 cut(s) 340, 1230
PaeI GCATGC 1 cut(s) 562
PaeR7I CTCGAG 2 cut(s) 192, 320
PagI TCATGA 4 cut(s) 7, 1033, 1345, 1942
PasI CCCWGGG 1 cut(s) 2034
PauI GCGCGC 2 cut(s) 201, 203
PceI AGGCCT 1 cut(s) 1168
PciI ACATGT 1 cut(s) 2124
PciSI GCTCTTC 1 cut(s) 287
PcsI WCGNNNNNNNCGW 1 cut(s) 1236
PctI GAATGC 1 cut(s) 467
PdmI GAANNNNTTC 3 cut(s) 1056, 1142, 1694
PfeI GAWTC 7 cut(s) 4, 377, 1466, 1695, 1987, 2069, 2213
PflMI CCANNNNNTGG 1 cut(s) 1013
PfoI TCCNGGA 1 cut(s) 1743
Ple19I CGATCG 1 cut(s) 2306
PleI GAGTC 5 cut(s) 51, 657, 1789, 2017, 2065
PpsI GAGTC 5 cut(s) 51, 657, 1789, 2017, 2065
PscI ACATGT 1 cut(s) 2124
Psp124BI GAGCTC 1 cut(s) 1338
Psp6I CCWGG 6 cut(s) 174, 302, 400, 1065, 1743, 2033
PspFI CCCAGC 1 cut(s) 2135
PspGI CCWGG 6 cut(s) 174, 302, 400, 1065, 1743, 2033
PspN4I GGNNCC 6 cut(s) 520, 1101, 1225, 1308, 1890, 2134
PspPI GGNCC 3 cut(s) 1741, 2132, 2201
PstI CTGCAG 2 cut(s) 1156, 1609
PstNI CAGNNNCTG 1 cut(s) 255
PsuI RGATCY 2 cut(s) 808, 1624
PteI GCGCGC 2 cut(s) 201, 203
PvuI CGATCG 1 cut(s) 2306
PvuII CAGCTG 2 cut(s) 86, 1838
RsaI GTAC 2 cut(s) 1438, 1874
RsaNI GTAC 2 cut(s) 1437, 1873
RseI CAYNNNNRTG 2 cut(s) 557, 1085
SacI GAGCTC 1 cut(s) 1338
SapI GCTCTTC 1 cut(s) 287
SaqAI TTAA 5 cut(s) 512, 567, 1278, 1499, 2241
Sau96I GGNCC 3 cut(s) 1741, 2132, 2201
SchI GAGTC 5 cut(s) 52, 658, 1790, 2017, 2066
ScrFI CCNGG 6 cut(s) 176, 304, 402, 1067, 1745, 2035
SduI GDGCHC 2 cut(s) 688, 1338
SfaNI GCATC 4 cut(s) 463, 661, 734, 916
SfcI CTRYAG 3 cut(s) 903, 1152, 1605
Sfr274I CTCGAG 2 cut(s) 192, 320
SfuI TTCGAA 2 cut(s) 340, 1230
SinI GGWCC 2 cut(s) 1741, 2132
SlaI CTCGAG 2 cut(s) 192, 320
SmiMI CAYNNNNRTG 2 cut(s) 557, 1085
SmlI CTYRAG 3 cut(s) 192, 320, 857
SmoI CTYRAG 3 cut(s) 192, 320, 857
SphI GCATGC 1 cut(s) 562
SseBI AGGCCT 1 cut(s) 1168
SsiI CCGC 5 cut(s) 247, 275, 617, 1819, 1910
SspI AATATT 1 cut(s) 1582
SspMI CTAG 3 cut(s) 485, 1302, 1661
SstI GAGCTC 1 cut(s) 1338
StuI AGGCCT 1 cut(s) 1168
StyD4I CCNGG 6 cut(s) 174, 302, 400, 1065, 1743, 2033
TaaI ACNGT 3 cut(s) 891, 1420, 1758
TaiI ACGT 2 cut(s) 134, 987
TaqI TCGA 7 cut(s) 193, 321, 340, 716, 1230, 2287, 2306
TauI GCSGC 1 cut(s) 278
TfiI GAWTC 7 cut(s) 4, 377, 1466, 1695, 1987, 2069, 2213
Tru1I TTAA 5 cut(s) 512, 567, 1278, 1499, 2241
Tru9I TTAA 5 cut(s) 512, 567, 1278, 1499, 2241
TscAI CASTG 7 cut(s) 554, 559, 852, 1014, 1459, 1705, 1997
TseFI GTSAC 4 cut(s) 878, 1371, 1447, 1827
TseI GCWGC 9 cut(s) 86, 494, 497, 722, 1018, 1151, 1154, 1271, 1604
Tsp45I GTSAC 4 cut(s) 878, 1371, 1447, 1827
TspDTI ATGAA 6 cut(s) 29, 455, 926, 1946, 2055, 2264
TspGWI ACGGA 1 cut(s) 977
TspRI CASTG 7 cut(s) 554, 559, 852, 1014, 1459, 1705, 1997
Van91I CCANNNNNTGG 1 cut(s) 1013
VpaK11BI GGWCC 2 cut(s) 1741, 2132
XagI CCTNNNNNAGG 1 cut(s) 934
XapI RAATTY 4 cut(s) 18, 800, 1724, 1933
XceI RCATGY 3 cut(s) 467, 562, 2128
XhoI CTCGAG 2 cut(s) 192, 320
XmiI GTMKAC 1 cut(s) 747
XmnI GAANNNNTTC 3 cut(s) 1056, 1142, 1694
XspI CTAG 3 cut(s) 485, 1302, 1661
ZraI GACGTC 1 cut(s) 985
Zsp2I ATGCAT 2 cut(s) 469, 1198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.