Rroxscaffold_4G00301490

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000598
Physical Location & Seq
Reverse (-)
21849312 .. 21849960
649 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00301490.1

Sequence Viewer

Length: 465 bp
ATGGTCTTCAACCACCACAGCTGCGGCTTCTCCTCCTCCAAATTCCAAATCAATCCCTTCTTCAGACTAACATCTTTATCTCGTATCCAGAAATTACAGCTTTTCACAAGCAAGTTTCAGGTTGGTACTCCAGTTACATTGGTCACTGGAGAGAACAAGCTAACTGGCTTGGTATATGTTAATGAGCACTTTGATGGGTGGAAGGCAGAGTGCTTGAAAATACTTCAAATTCACTTTAACAGTGACACTCGTACTTTTTCTTCAGACAAGTCGATACAGGAGGCGTTACAGAAAAGTTCTATTGTCACTCTAAAGATTTTCGATGGAGAAACCCTGAAGTTGTGTATGCCTTTTATGAAGTCAAAGAAGGATGAAGCAATTGCGCTTGGGGCTCGGGCCTTGGACTTAACGTTACCATTTGGAGAGATTGAGGTCCTTCTGTCCTTCATGAGAACTTGGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

154

Amino Acids

17.49

Weight (kDa)

9.05

Isoelectric Point (pI)

34.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RBD_LARS1 PF24810 61 - 138 2.9e-06 Leucine--tRNA ligase, RagD-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000598)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09620
fragaria_vesca FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_3g34581 FvH4_4g33720 FvH4_4g33720
malus_domestica MD08G1244600.v1.1 MD15G1439400.v1.1
prunus_persica Prupe.1G582000_v2.0.a1 Prupe.2G015100_v2.0.a1 Prupe.2G123100_v2.0.a1
pyrus_communis pycom08g21210 pycom08g21220 pycom15g38730
rosa_chinensis RchiOBHm_Chr1g0336121 RchiOBHm_Chr1g0353811 RchiOBHm_Chr1g0373331 RchiOBHm_Chr4g0443191 RchiOBHm_Chr4g0443201 RchiOBHm_Chr4g0443211
rosa_laevigata RLG00000003122 RLG00000005948 RLG00000026826 RLG00000028251 RLG00000028252 RLG00000028253 RLG00000029377
rosa_multiflora Rmu_co8002498.1_g000001 Rmu_co8262279.1_g000001 Rmu_co8314139.1_g000001 Rmu_co8393711.1_g000001 Rmu_co8419167.1_g000001 Rmu_sc0007968.1_g000004 Rmu_sc0018919.1_g000004 Rmu_sc0027447.1_g000001
rosa_roxburghii Rroxscaffold_4G00284260 Rroxscaffold_4G00301490 Rroxscaffold_4G00315860 Rroxscaffold_5G00383790 Rroxscaffold_5G00383800
rosa_rugosa Rorug01G0123100 Rorug01G0238700 Rorug01G0377100 Rorug01G0377100 Rorug01G0377200 Rorug01G0377300 Rorug04G0344700 Rorug05G0347900.1
rosa_samantha Rh1AG143800 Rh1AG250300 Rh1AG386300 Rh1BG113400 Rh1BG220800 Rh1BG220900 Rh1BG350400 Rh1CG135500 Rh1CG233100 Rh1CG363500 Rh1DG150200 Rh1DG247700 Rh1DG247800 Rh1DG381300 Rh4AG399400 Rh4AG399500 Rh4BG411000 Rh4BG411100 Rh4BG411200 Rh4CG426500 Rh4CG426600 Rh4DG406100 Rh4DG406200 Rh4DG406300
rosa_wichuraiana Rw0G015190 Rw1G021850 Rw1G034870 Rw4G034440 Rw4G034450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 24
AclI AACGTT 1 cut(s) 410
AcsI RAATTY 2 cut(s) 41, 228
AcuI CTGAAG 3 cut(s) 46, 246, 356
AfaI GTAC 2 cut(s) 127, 253
AgsI TTSAA 3 cut(s) 10, 217, 227
AluBI AGCT 3 cut(s) 21, 100, 160
AluI AGCT 3 cut(s) 21, 100, 160
Alw21I GWGCWC 1 cut(s) 189
Ama87I CYCGRG 1 cut(s) 393
AoxI GGCC 1 cut(s) 396
ApeKI GCWGC 1 cut(s) 21
ApoI RAATTY 2 cut(s) 41, 228
AspLEI GCGC 1 cut(s) 385
AspS9I GGNCC 2 cut(s) 396, 433
AvaI CYCGRG 1 cut(s) 393
AvaII GGWCC 1 cut(s) 433
BaeI ACNNNNGTAYC 2 cut(s) 117, 150
BanII GRGCYC 1 cut(s) 394
BarI GAAGNNNNNNTAC 2 cut(s) 244, 276
Bbv12I GWGCWC 1 cut(s) 189
BbvI GCAGC 1 cut(s) 8
BccI CCATC 2 cut(s) 188, 317
BciVI GTATCC 1 cut(s) 95
BfuI GTATCC 1 cut(s) 95
BisI GCNGC 2 cut(s) 22, 25
BlsI GCNGC 2 cut(s) 23, 26
Bme18I GGWCC 1 cut(s) 433
BmeT110I CYCGRG 1 cut(s) 393
BmgT120I GGNCC 2 cut(s) 396, 433
BpmI CTGGAG 2 cut(s) 114, 168
BsaJI CCNNGG 1 cut(s) 399
Bse1I ACTGG 3 cut(s) 131, 151, 169
BseDI CCNNGG 1 cut(s) 399
BseGI GGATG 1 cut(s) 376
BseNI ACTGG 3 cut(s) 131, 151, 169
BseRI GAGGAG 2 cut(s) 22, 25
BseXI GCAGC 1 cut(s) 8
BshFI GGCC 1 cut(s) 398
BsiHKAI GWGCWC 1 cut(s) 189
BsiHKCI CYCGRG 1 cut(s) 393
BsnI GGCC 1 cut(s) 398
BsoBI CYCGRG 1 cut(s) 393
Bsp1286I GDGCHC 2 cut(s) 189, 394
BspACI CCGC 1 cut(s) 24
BspANI GGCC 1 cut(s) 398
BspHI TCATGA 1 cut(s) 447
BsrI ACTGG 3 cut(s) 131, 151, 169
BssECI CCNNGG 1 cut(s) 399
BssT1I CCWWGG 1 cut(s) 399
Bst4CI ACNGT 1 cut(s) 242
BstF5I GGATG 1 cut(s) 376
BstHHI GCGC 1 cut(s) 385
BstMWI GCNNNNNNNGC 1 cut(s) 389
BstV1I GCAGC 1 cut(s) 8
BsuI GTATCC 1 cut(s) 95
BsuRI GGCC 1 cut(s) 398
BtsCI GGATG 1 cut(s) 376
BtsIMutI CAGTG 2 cut(s) 144, 247
CciI TCATGA 1 cut(s) 447
CfoI GCGC 1 cut(s) 385
Cfr13I GGNCC 2 cut(s) 396, 433
Csp6I GTAC 2 cut(s) 126, 252
CviAII CATG 1 cut(s) 448
CviJI RGCY 7 cut(s) 21, 27, 100, 160, 168, 392, 398
CviKI_1 RGCY 7 cut(s) 21, 27, 100, 160, 168, 392, 398
CviQI GTAC 2 cut(s) 126, 252
Eco130I CCWWGG 1 cut(s) 399
Eco24I GRGCYC 1 cut(s) 394
Eco47I GGWCC 1 cut(s) 433
Eco57I CTGAAG 3 cut(s) 46, 246, 356
Eco88I CYCGRG 1 cut(s) 393
EcoO109I RGGNCCY 1 cut(s) 433
EcoT14I CCWWGG 1 cut(s) 399
EcoT38I GRGCYC 1 cut(s) 394
ErhI CCWWGG 1 cut(s) 399
FaeI CATG 1 cut(s) 451
FaiI YATR 5 cut(s) 175, 177, 347, 356, 449
FatI CATG 1 cut(s) 447
Fnu4HI GCNGC 2 cut(s) 22, 25
FokI GGATG 1 cut(s) 383
FriOI GRGCYC 1 cut(s) 394
Fsp4HI GCNGC 2 cut(s) 22, 25
GlaI GCGC 1 cut(s) 384
GluI GCNGC 2 cut(s) 22, 25
GsuI CTGGAG 2 cut(s) 114, 168
HaeIII GGCC 1 cut(s) 398
HhaI GCGC 1 cut(s) 385
Hin1II CATG 1 cut(s) 451
Hin6I GCGC 1 cut(s) 383
HinP1I GCGC 1 cut(s) 383
Hpy188I TCNGA 2 cut(s) 65, 265
Hpy188III TCNNGA 2 cut(s) 88, 448
HpyAV CCTTC 5 cut(s) 67, 196, 361, 446, 454
HpyCH4III ACNGT 1 cut(s) 242
HpyCH4IV ACGT 1 cut(s) 410
HpyF10VI GCNNNNNNNGC 1 cut(s) 389
HpySE526I ACGT 1 cut(s) 410
Hsp92II CATG 1 cut(s) 451
HspAI GCGC 1 cut(s) 383
LpnPI CCDG 7 cut(s) 101, 104, 132, 144, 150, 263, 347
Lsp1109I GCAGC 1 cut(s) 8
MaeII ACGT 1 cut(s) 410
MaeIII GTNAC 6 cut(s) 133, 142, 242, 285, 304, 411
MboII GAAGA 2 cut(s) 52, 252
MfeI CAATTG 1 cut(s) 378
MhlI GDGCHC 2 cut(s) 189, 394
MluCI AATT 4 cut(s) 41, 92, 228, 378
MnlI CCTC 4 cut(s) 43, 46, 274, 424
MseI TTAA 3 cut(s) 180, 237, 407
MslI CAYNNNNRTG 1 cut(s) 192
MspA1I CMGCKG 1 cut(s) 21
MunI CAATTG 1 cut(s) 378
MwoI GCNNNNNNNGC 1 cut(s) 389
NlaIII CATG 1 cut(s) 451
NmuCI GTSAC 3 cut(s) 142, 242, 304
PagI TCATGA 1 cut(s) 447
PkrI GCNGC 2 cut(s) 23, 26
PpuMI RGGWCCY 1 cut(s) 433
Psp1406I AACGTT 1 cut(s) 410
Psp5II RGGWCCY 1 cut(s) 433
PspPI GGNCC 2 cut(s) 396, 433
PspPPI RGGWCCY 1 cut(s) 433
PvuII CAGCTG 1 cut(s) 21
RsaI GTAC 2 cut(s) 127, 253
RsaNI GTAC 2 cut(s) 126, 252
RseI CAYNNNNRTG 1 cut(s) 192
SaqAI TTAA 3 cut(s) 180, 237, 407
SatI GCNGC 2 cut(s) 22, 25
Sau96I GGNCC 2 cut(s) 396, 433
SduI GDGCHC 2 cut(s) 189, 394
SetI ASST 6 cut(s) 23, 102, 123, 162, 413, 435
SinI GGWCC 1 cut(s) 433
SmiMI CAYNNNNRTG 1 cut(s) 192
Sse9I AATT 4 cut(s) 41, 92, 228, 378
SsiI CCGC 1 cut(s) 24
StyI CCWWGG 1 cut(s) 399
TaaI ACNGT 1 cut(s) 242
TaiI ACGT 1 cut(s) 413
TaqI TCGA 2 cut(s) 272, 321
TasI AATT 4 cut(s) 41, 92, 228, 378
TauI GCSGC 1 cut(s) 27
Tru1I TTAA 3 cut(s) 180, 237, 407
Tru9I TTAA 3 cut(s) 180, 237, 407
TscAI CASTG 2 cut(s) 151, 247
TseFI GTSAC 3 cut(s) 142, 242, 304
TseI GCWGC 1 cut(s) 21
Tsp45I GTSAC 3 cut(s) 142, 242, 304
TspDTI ATGAA 3 cut(s) 371, 387, 436
TspRI CASTG 2 cut(s) 151, 247
VpaK11BI GGWCC 1 cut(s) 433
XapI RAATTY 2 cut(s) 41, 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.