Rh4BG411200

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000598
Physical Location & Seq
Reverse (-)
56014414 .. 56018958
4545 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG411200.1

Sequence Viewer

Length: 3057 bp
ATGGCAATCGAAGGTGGGAAGAAATCTAGGAGAGATCGTCTCAAAGCGATTGAGGAGAAGGCTCAAAAGTTGTGGGAAGAAAATGGTGTTTTCAGGGCTGAATCTTGTGAAAAGCCCCCTGAACCAGGGGAGAAGTTCTTTGGGAACTTCCCTCTTCCTTACATGAATGGTTTTTTGCATCTAGGGCATGCATTCTCACTCTCGAAGCTAGAGTTTTCTGCTCGTTTTCATAGGTTAAGAGGTGCTAATGTGCTACTCCCGGTTGGTTTCCACCTCACTGGCATGCCCATCAAAAGTATTGGCCTGTCTGCCATTGATATATCAGAACTCCAGAATCCATCGAACTGGTTGAGCTACTTCCCACGAGTTGCAGTGAAAGAGCTCAAGGCATTTGGCTTGGGCTGTGACTGGAGGCGCTCCTTTATTACCACAGACATGAACCCATACTTTGACAAGTTTGTGAGATGGCAGATGAGGAAACTAAAATCTATGGGTAAGATTGTGAAGGATGTCCGTTACACAATCTACTCTCCTTTGGATGGCCAGCCATGTGCAGATCATGATAGGGCAACTGGTGAAGGAGTTCGGCCCCAAGAGTACACCATCATCAAAATGGAGGTGGTGGCACCTTTTCCTCCTAAACTGGGAGTGTTGAAGGGAAAGAAAGTCTTCCTTGCTGCCGCAACATTGAGACCTGAGACTTCTTATGGTCAAACAAATGCATGGGTGTTGCCTGATGGCAAGTATGGAGCCTTTGAAATCAATGAAACAGATGTCTTCATTCTTACGCAGAGAGCAGGACTTAATCTTGCCTATCAGAAGTACTCTAGGGTTCCAGAAAAGCCTTCTTGTTTGGTTGAGATGACTGGCTATGATTTGATTGGTCTTCCATTGAAGTCTCCACTTGCACTCAATCAGATCGTTTATGCCCTTCCTATGTTGACCGTCCTAACAGACAAAGGTACTGGGATATTGACTAGTGTACCTAGTGATGCTCCTGATGATTATATGGCCTTGGAAGATTTAAAATTGAAACCAGCTTTTAGAGAAAAATATGGTGTGAAGGATGAATGGGTCATGCTCTTTGATATCATTCCAATAATCAAAATTCCGGAATATGGAGACAAGGCTGCTAAAAAGGTTGTGGAAGATCTCAAAATCAAAAGTCAGAATGAGATAGAGAAGCTAGCAGAAGCCAAGAGGTTGACATCCTTGAAAGGTTTAACTGAAGGAAAACTGCTTGTGGGAGAATTCAAAGAAAGGATAGTCCAGGAGGCAAAACCATTGATAAGGAGCAGGCTATTAGAGGCAGGTGATGCAATCATGTATAGCGAGCCTGAAAAGCAGGTAGTTTCACGATCTGGTGATGAATGTGTTGTTGCTCTTATAGATCAATGGTGCATCACATATGGGGAAGCAGAATGGAAAAAAACTGCTCAGGAGTGCTTGTCATGCATAAATTTATATTCTGACACAACACGACATGGATTTGTACACACATTGAATTGGTTGAATCAGTGGGCTTGTTCTCGATCTTTTGGGCTTGGTACACGCATTCCCTGGGATGAAGAATACTTGGTCGAGCCGTTATCCGATTCAACTCTTTACATGGCTTATTACACCATTGCTCAACTGCTACACAATGAAGACATGTATGGCAGCTCCAACAGCACATCTGCAGTAAGAGCTGAACAAATGACCGATGAGGTTTGGGATTTTATTTTCTGTGATGGCTCGTATCCACAATCATCTGATATCACACCATCAATTCTTAACAAGATGAAGCAGGAGTTTGAATATTGGTATCCTTTTGATCTTGAGGTGTCTGGTAAGGATCTAATCCAGAATCATACCTTCTGCATTTACAACCATACTGCACTTTTGCCCAAGAAGCACTGGCCTAGTGCGTTTAGATGCAATGGGCACCTGATGCTCAAATCCACGAAGTATGTTAATGGTGCTGGAAATTTTATATCATTGAGACACGCAATTGATCACTATTCTGCTGATGCCACGCGGTTTGCTTTGGCTGATATTAGTGATGGTATTGACGATGCATACTTTTCATTTGAAACTGCAAATGCCGCCATCATACGTCTCACTAAGGAGATAGATTGGATTGATCGGATGAAGGATAAAGATTTGGGTAAAGACTCTTCTCTGAGAATAGGTTCCCCTTGTACCTTCTCTGACCGGGTCTTTCTGAATGAAATAAATATTGCTGTGAAGAGGACTGAGCAAAATTACCAGGGTTGCATGTTCAGGGAAGCCCTAAAGACTGGCTTTTACGATCTGCAAGCTGCCAGGGATTGGTACAGGATTTCATGTGGTGGTACTAATGCAATGAACCACGATTTGGTGTGGCGTTTTATAGATGTGCAGACCCGTCTTATTGCTCCAATTTGTCCCCACTATGCAGAACATGTCTGGAGGGAACTTTTAGAGAGGGAAGGATTTGCAGTAAATGCAGGCTGGCCCGCGGCTGATGCTCCAGATCTAACACTCCAGAGTGCGAATAAGTATTTGCAAGACTCAATTGATTCAATAAGGAAGCAGCATGATAAACAACTATCAACTTCAAAGAAAACCAATATGGGTGCTTCAGCTACTTCTTTGACAGAAGCGTTGATATATGTTAAGGAGAAATATGACGGATGGAAAGCAGAATGCTTAAGAATACTCCAACATGACTTAAATAGAGATACTCTTACTTCTGCTCACGAGGACAAGGAGATATTTGAGGCATTGAAGGCTAGTTCTTTTGGTCAAGATGATGATTTTAGCCAAAAGAATTGTATGGAATTTTTGAAGTTCAAGAAGGAGAAGGCAGTTGCACTTGGGATTCAGGCATTGGACTTGAAGCTACCTTTCGGAGAGATTGAAGTCCTTGAACAAAACTTGGACTTGATTAAAAAACAAATACATGGTCTTGAAGAGGTACAAGTTTTGTCAGCTATCAATGCTGCTGATATTGCTAGAGCTGGTCCTCATGCTAGACTGCTAGAGAAGAATCCTCCCTCTCCTGGAAGCCCAACTACGGTTTTCTTGACCCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

1018

Amino Acids

115.34

Weight (kDa)

6.19

Isoelectric Point (pI)

41.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1 PF00133 22 - 686 4.5e-21 tRNA synthetases class I (I, L, M and V)
Anticodon_1 PF08264 731 - 854 1.6e-16 Anticodon-binding domain of tRNA ligase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000598)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09620
fragaria_vesca FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_3g34581 FvH4_4g33720 FvH4_4g33720
malus_domestica MD08G1244600.v1.1 MD15G1439400.v1.1
prunus_persica Prupe.1G582000_v2.0.a1 Prupe.2G015100_v2.0.a1 Prupe.2G123100_v2.0.a1
pyrus_communis pycom08g21210 pycom08g21220 pycom15g38730
rosa_chinensis RchiOBHm_Chr1g0336121 RchiOBHm_Chr1g0353811 RchiOBHm_Chr1g0373331 RchiOBHm_Chr4g0443191 RchiOBHm_Chr4g0443201 RchiOBHm_Chr4g0443211
rosa_laevigata RLG00000003122 RLG00000005948 RLG00000026826 RLG00000028251 RLG00000028252 RLG00000028253 RLG00000029377
rosa_multiflora Rmu_co8002498.1_g000001 Rmu_co8262279.1_g000001 Rmu_co8314139.1_g000001 Rmu_co8393711.1_g000001 Rmu_co8419167.1_g000001 Rmu_sc0007968.1_g000004 Rmu_sc0018919.1_g000004 Rmu_sc0027447.1_g000001
rosa_roxburghii Rroxscaffold_4G00284260 Rroxscaffold_4G00301490 Rroxscaffold_4G00315860 Rroxscaffold_5G00383790 Rroxscaffold_5G00383800
rosa_rugosa Rorug01G0123100 Rorug01G0238700 Rorug01G0377100 Rorug01G0377100 Rorug01G0377200 Rorug01G0377300 Rorug04G0344700 Rorug05G0347900.1
rosa_samantha Rh1AG143800 Rh1AG250300 Rh1AG386300 Rh1BG113400 Rh1BG220800 Rh1BG220900 Rh1BG350400 Rh1CG135500 Rh1CG233100 Rh1CG363500 Rh1DG150200 Rh1DG247700 Rh1DG247800 Rh1DG381300 Rh4AG399400 Rh4AG399500 Rh4BG411000 Rh4BG411100 Rh4BG411200 Rh4CG426500 Rh4CG426600 Rh4DG406100 Rh4DG406200 Rh4DG406300
rosa_wichuraiana Rw0G015190 Rw1G021850 Rw1G034870 Rw4G034440 Rw4G034450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1301
Acc36I ACCTGC 2 cut(s) 1301, 1336
AccB1I GGYRCC 2 cut(s) 625, 1923
AccB7I CCANNNNNTGG 2 cut(s) 2310, 2356
AccII CGCG 2 cut(s) 2017, 2480
AccIII TCCGGA 1 cut(s) 1111
AciI CCGC 5 cut(s) 681, 2017, 2085, 2478, 2480
AclWI GGATC 1 cut(s) 1842
AcoI YGGCCR 1 cut(s) 541
AcsI RAATTY 5 cut(s) 1107, 1250, 1459, 1966, 2801
AcuI CTGAAG 2 cut(s) 1248, 2586
AfiI CCNNNNNNNGG 8 cut(s) 125, 539, 644, 1118, 2310, 2356, 3023, 3037
AflII CTTAAG 1 cut(s) 2671
AflIII ACRYGT 2 cut(s) 1650, 2422
AhlI ACTAGT 1 cut(s) 977
AjnI CCWGG 6 cut(s) 124, 1269, 1559, 2247, 2303, 3022
AjuI GAANNNNNNNTTGG 2 cut(s) 2339, 2371
Alw21I GWGCWC 1 cut(s) 384
Alw26I GTCTC 7 cut(s) 44, 685, 692, 903, 1116, 1975, 2102
AlwI GGATC 1 cut(s) 1842
Aor13HI TCCGGA 1 cut(s) 1111
AoxI GGCC 6 cut(s) 301, 541, 587, 1011, 1898, 2474
ApeKI GCWGC 6 cut(s) 677, 1130, 1659, 2300, 2554, 2963
ApoI RAATTY 5 cut(s) 1107, 1250, 1459, 1966, 2801
ArsI GACNNNNNNTTYG 2 cut(s) 2911, 2943
Asp700I GAANNNNTTC 3 cut(s) 582, 668, 2170
AspLEI GCGC 1 cut(s) 417
AspS9I GGNCC 3 cut(s) 588, 2475, 2984
AsuC2I CCSGG 3 cut(s) 260, 2195, 3052
AsuHPI GGTGA 3 cut(s) 587, 1325, 1376
AsuNHI GCTAGC 1 cut(s) 1186
AvaII GGWCC 1 cut(s) 2984
BaeGI GKGCMC 1 cut(s) 1926
BaeI ACNNNNGTAYC 2 cut(s) 966, 999
BalI TGGCCA 1 cut(s) 543
BanI GGYRCC 2 cut(s) 625, 1923
BanII GRGCYC 1 cut(s) 384
BarI GAAGNNNNNNTAC 4 cut(s) 2140, 2172, 3019, 3051
BauI CACGAG 2 cut(s) 363, 2720
BbsI GAAGAC 4 cut(s) 661, 769, 878, 1653
Bbv12I GWGCWC 1 cut(s) 384
BbvI GCAGC 6 cut(s) 664, 1117, 1671, 2287, 2566, 2950
BceAI ACGGC 1 cut(s) 1570
BciT130I CCWGG 6 cut(s) 126, 1271, 1561, 2249, 2305, 3024
BciVI GTATCC 2 cut(s) 1749, 1815
BclI TGATCA 1 cut(s) 1993
BcnI CCSGG 3 cut(s) 260, 2195, 3052
BcoDI GTCTC 7 cut(s) 44, 685, 692, 903, 1116, 1975, 2102
BcuI ACTAGT 1 cut(s) 977
BfmI CTRYAG 1 cut(s) 1677
BfoI RGCGCY 1 cut(s) 418
BfrI CTTAAG 1 cut(s) 2671
BfuAI ACCTGC 2 cut(s) 1301, 1336
BfuI GTATCC 2 cut(s) 1749, 1815
BglII AGATCT 2 cut(s) 1150, 2494
BisI GCNGC 9 cut(s) 678, 681, 1131, 1660, 2085, 2301, 2481, 2555, 2964
BlsI GCNGC 9 cut(s) 679, 682, 1132, 1661, 2086, 2302, 2482, 2556, 2965
BmcAI AGTACT 1 cut(s) 824
Bme1390I CCNGG 9 cut(s) 126, 260, 1271, 1561, 2195, 2249, 2305, 3024, 3052
Bme18I GGWCC 1 cut(s) 2984
BmgT120I GGNCC 3 cut(s) 588, 2475, 2984
BmiI GGNNCC 6 cut(s) 590, 627, 751, 834, 1925, 2173
BmrFI CCNGG 9 cut(s) 126, 260, 1271, 1561, 2195, 2249, 2305, 3024, 3052
BmrI ACTGGG 2 cut(s) 653, 975
BmsI GCATC 9 cut(s) 187, 982, 1306, 1410, 1904, 1920, 1999, 2044, 2476
BmtI GCTAGC 1 cut(s) 1190
BmuI ACTGGG 2 cut(s) 653, 975
BpiI GAAGAC 4 cut(s) 661, 769, 878, 1653
BplI GAGNNNNNCTC 2 cut(s) 24, 56
BpmI CTGGAG 5 cut(s) 314, 430, 2449, 2475, 2489
Bpu10I CCTNAGC 1 cut(s) 1437
BpuEI CTTGAG 2 cut(s) 368, 1838
BpuMI CCSGG 3 cut(s) 260, 2195, 3052
BsaI GGTCTC 1 cut(s) 685
BsaJI CCNNGG 7 cut(s) 125, 1014, 1559, 1560, 2248, 2304, 2478
BsaWI WCCGGW 1 cut(s) 1111
BsaXI ACNNNNNCTCC 4 cut(s) 22, 52, 1433, 1463
Bsc4I CCNNNNNNNGG 8 cut(s) 125, 539, 644, 1118, 2310, 2356, 3023, 3037
Bse1I ACTGG 9 cut(s) 283, 350, 413, 577, 648, 871, 970, 1901, 2284
Bse3DI GCAATG 3 cut(s) 1623, 1924, 2349
BseAI TCCGGA 1 cut(s) 1111
BseBI CCWGG 6 cut(s) 126, 1271, 1561, 2249, 2305, 3024
BseDI CCNNGG 7 cut(s) 125, 1014, 1559, 1560, 2248, 2304, 2478
BseGI GGATG 7 cut(s) 514, 544, 1072, 1208, 1570, 2133, 2660
BseLI CCNNNNNNNGG 8 cut(s) 125, 539, 644, 1118, 2310, 2356, 3023, 3037
BseMI GCAATG 3 cut(s) 1623, 1924, 2349
BseMII CTCAG 4 cut(s) 687, 1451, 2153, 2226
BseNI ACTGG 9 cut(s) 283, 350, 413, 577, 648, 871, 970, 1901, 2284
BseRI GAGGAG 1 cut(s) 68
BseSI GKGCMC 1 cut(s) 1926
BseXI GCAGC 6 cut(s) 664, 1117, 1671, 2287, 2566, 2950
BsgI GTGCAG 3 cut(s) 573, 1860, 2399
Bsh1236I CGCG 2 cut(s) 2017, 2480
BshFI GGCC 6 cut(s) 303, 543, 589, 1013, 1900, 2476
BshNI GGYRCC 2 cut(s) 625, 1923
BsiHKAI GWGCWC 1 cut(s) 384
BsiSI CCGG 4 cut(s) 260, 1112, 2194, 3052
BslFI GGGAC 1 cut(s) 2391
BslI CCNNNNNNNGG 8 cut(s) 125, 539, 644, 1118, 2310, 2356, 3023, 3037
BsmAI GTCTC 7 cut(s) 44, 685, 692, 903, 1116, 1975, 2102
BsmBI CGTCTC 2 cut(s) 44, 2102
BsmFI GGGAC 1 cut(s) 2391
BsmI GAATGC 3 cut(s) 191, 1554, 2672
BsnI GGCC 6 cut(s) 303, 543, 589, 1013, 1900, 2476
Bso31I GGTCTC 1 cut(s) 685
Bsp1286I GDGCHC 2 cut(s) 384, 1926
Bsp13I TCCGGA 1 cut(s) 1111
Bsp1407I TGTACA 1 cut(s) 1492
BspACI CCGC 5 cut(s) 681, 2017, 2085, 2478, 2480
BspANI GGCC 6 cut(s) 303, 543, 589, 1013, 1900, 2476
BspCNI CTCAG 4 cut(s) 688, 1450, 2154, 2227
BspEI TCCGGA 1 cut(s) 1111
BspFNI CGCG 2 cut(s) 2017, 2480
BspHI TCATGA 1 cut(s) 559
BspLI GGNNCC 6 cut(s) 590, 627, 751, 834, 1925, 2173
BspMAI CTGCAG 1 cut(s) 1681
BspMI ACCTGC 2 cut(s) 1301, 1336
BspOI GCTAGC 1 cut(s) 1190
BspPI GGATC 1 cut(s) 1842
BspT107I GGYRCC 2 cut(s) 625, 1923
BspTI CTTAAG 1 cut(s) 2671
BspTNI GGTCTC 1 cut(s) 685
BsrDI GCAATG 3 cut(s) 1623, 1924, 2349
BsrGI TGTACA 1 cut(s) 1492
BsrI ACTGG 9 cut(s) 283, 350, 413, 577, 648, 871, 970, 1901, 2284
BssECI CCNNGG 7 cut(s) 125, 1014, 1559, 1560, 2248, 2304, 2478
BssSI CACGAG 2 cut(s) 363, 2720
BssT1I CCWWGG 1 cut(s) 1014
Bst2BI CACGAG 2 cut(s) 363, 2720
Bst2UI CCWGG 6 cut(s) 126, 1271, 1561, 2249, 2305, 3024
Bst4CI ACNGT 2 cut(s) 946, 3040
Bst6I CTCTTC 4 cut(s) 159, 2161, 2222, 2928
BstAFI CTTAAG 1 cut(s) 2671
BstAPI GCANNNNNTGC 3 cut(s) 1316, 1930, 2465
BstAUI TGTACA 1 cut(s) 1492
BstDEI CTNAG 5 cut(s) 696, 1437, 2103, 2162, 2235
BstDSI CCRYGG 1 cut(s) 2478
BstENI CCTNNNNNAGG 1 cut(s) 123
BstF5I GGATG 7 cut(s) 514, 544, 1072, 1208, 1570, 2133, 2660
BstFNI CGCG 2 cut(s) 2017, 2480
BstH2I RGCGCY 1 cut(s) 418
BstHHI GCGC 1 cut(s) 417
BstMAI GTCTC 7 cut(s) 44, 685, 692, 903, 1116, 1975, 2102
BstNI CCWGG 6 cut(s) 126, 1271, 1561, 2249, 2305, 3024
BstNSI RCATGY 5 cut(s) 191, 286, 1654, 2260, 2426
BstSCI CCNGG 9 cut(s) 124, 258, 1269, 1559, 2193, 2247, 2303, 3022, 3050
BstSFI CTRYAG 1 cut(s) 1677
BstSLI GKGCMC 1 cut(s) 1926
BstUI CGCG 2 cut(s) 2017, 2480
BstV1I GCAGC 6 cut(s) 664, 1117, 1671, 2287, 2566, 2950
BstV2I GAAGAC 4 cut(s) 661, 769, 878, 1653
BstX2I RGATCY 3 cut(s) 1150, 1834, 2494
BstXI CCANNNNNNTGG 2 cut(s) 278, 345
BstYI RGATCY 3 cut(s) 1150, 1834, 2494
BsuI GTATCC 2 cut(s) 1749, 1815
BsuRI GGCC 6 cut(s) 303, 543, 589, 1013, 1900, 2476
BtgI CCRYGG 1 cut(s) 2478
BtsCI GGATG 7 cut(s) 514, 544, 1072, 1208, 1570, 2133, 2660
BtsI GCAGTG 1 cut(s) 378
BtsIMutI CAGTG 4 cut(s) 276, 378, 1523, 1894
BveI ACCTGC 2 cut(s) 1301, 1336
CciI TCATGA 1 cut(s) 559
CfoI GCGC 1 cut(s) 417
Cfr13I GGNCC 3 cut(s) 588, 2475, 2984
Cfr42I CCGCGG 1 cut(s) 2481
CspCI CAANNNNNGTGG 4 cut(s) 53, 88, 2002, 2037
DdeI CTNAG 5 cut(s) 696, 1437, 2103, 2162, 2235
DraI TTTAAA 1 cut(s) 1026
EaeI YGGCCR 1 cut(s) 541
Eam1104I CTCTTC 4 cut(s) 159, 2161, 2222, 2928
EarI CTCTTC 4 cut(s) 159, 2161, 2222, 2928
Ecl136II GAGCTC 1 cut(s) 382
Eco130I CCWWGG 1 cut(s) 1014
Eco24I GRGCYC 1 cut(s) 384
Eco31I GGTCTC 1 cut(s) 685
Eco32I GATATC 2 cut(s) 1090, 1756
Eco47I GGWCC 1 cut(s) 2984
Eco53kI GAGCTC 1 cut(s) 382
Eco57I CTGAAG 2 cut(s) 1248, 2586
EcoICRI GAGCTC 1 cut(s) 382
EcoNI CCTNNNNNAGG 1 cut(s) 123
EcoRI GAATTC 1 cut(s) 1250
EcoRII CCWGG 6 cut(s) 124, 1269, 1559, 2247, 2303, 3022
EcoRV GATATC 2 cut(s) 1090, 1756
EcoT14I CCWWGG 1 cut(s) 1014
EcoT22I ATGCAT 4 cut(s) 193, 724, 1457, 2059
EcoT38I GRGCYC 1 cut(s) 384
ErhI CCWWGG 1 cut(s) 1014
Esp3I CGTCTC 2 cut(s) 44, 2102
FalI AAGNNNNNCTT 6 cut(s) 653, 685, 657, 689, 2267, 2299
FaqI GGGAC 1 cut(s) 2391
FauI CCCGC 1 cut(s) 2485
FauNDI CATATG 1 cut(s) 1408
FbaI TGATCA 1 cut(s) 1993
Fnu4HI GCNGC 9 cut(s) 678, 681, 1131, 1660, 2085, 2301, 2481, 2555, 2964
FokI GGATG 7 cut(s) 521, 551, 1079, 1195, 1577, 2140, 2667
FriOI GRGCYC 1 cut(s) 384
Fsp4HI GCNGC 9 cut(s) 678, 681, 1131, 1660, 2085, 2301, 2481, 2555, 2964
GlaI GCGC 1 cut(s) 416
GluI GCNGC 9 cut(s) 678, 681, 1131, 1660, 2085, 2301, 2481, 2555, 2964
GsuI CTGGAG 5 cut(s) 314, 430, 2449, 2475, 2489
HaeII RGCGCY 1 cut(s) 418
HaeIII GGCC 6 cut(s) 303, 543, 589, 1013, 1900, 2476
HapII CCGG 4 cut(s) 260, 1112, 2194, 3052
HhaI GCGC 1 cut(s) 417
Hin6I GCGC 1 cut(s) 415
HinP1I GCGC 1 cut(s) 415
HincII GTYRAC 2 cut(s) 942, 1206
HindII GTYRAC 2 cut(s) 942, 1206
HpaII CCGG 4 cut(s) 260, 1112, 2194, 3052
HphI GGTGA 3 cut(s) 587, 1325, 1376
Hpy166II GTNNAC 6 cut(s) 600, 942, 983, 1206, 1495, 1550
Hpy8I GTNNAC 6 cut(s) 600, 942, 983, 1206, 1495, 1550
HpyCH4III ACNGT 2 cut(s) 946, 3040
HpyCH4IV ACGT 1 cut(s) 2095
HpyF3I CTNAG 5 cut(s) 696, 1437, 2103, 2162, 2235
HpySE526I ACGT 1 cut(s) 2095
HspAI GCGC 1 cut(s) 415
Kpn2I TCCGGA 1 cut(s) 1111
Ksp22I TGATCA 1 cut(s) 1993
KspI CCGCGG 1 cut(s) 2481
LmnI GCTCC 7 cut(s) 422, 749, 1000, 1293, 1667, 2401, 2494
Lsp1109I GCAGC 6 cut(s) 664, 1117, 1671, 2287, 2566, 2950
LweI GCATC 9 cut(s) 187, 982, 1306, 1410, 1904, 1920, 1999, 2044, 2476
MaeII ACGT 1 cut(s) 2095
MaeIII GTNAC 2 cut(s) 404, 515
MfeI CAATTG 2 cut(s) 1989, 2535
MflI RGATCY 3 cut(s) 1150, 1834, 2494
MhlI GDGCHC 2 cut(s) 384, 1926
MlsI TGGCCA 1 cut(s) 543
MluNI TGGCCA 1 cut(s) 543
MlyI GAGTC 2 cut(s) 2147, 2525
MmeI TCCRAC 2 cut(s) 1689, 2707
Mox20I TGGCCA 1 cut(s) 543
Mph1103I ATGCAT 4 cut(s) 193, 724, 1457, 2059
MroI TCCGGA 1 cut(s) 1111
MroXI GAANNNNTTC 3 cut(s) 582, 668, 2170
MscI TGGCCA 1 cut(s) 543
MslI CAYNNNNRTG 3 cut(s) 281, 434, 611
Msp20I TGGCCA 1 cut(s) 543
MspA1I CMGCKG 1 cut(s) 2480
MspCI CTTAAG 1 cut(s) 2671
MspI CCGG 4 cut(s) 260, 1112, 2194, 3052
MspR9I CCNGG 9 cut(s) 126, 260, 1271, 1561, 2195, 2249, 2305, 3024, 3052
MunI CAATTG 2 cut(s) 1989, 2535
Mva1269I GAATGC 3 cut(s) 191, 1554, 2672
MvaI CCWGG 6 cut(s) 126, 1271, 1561, 2249, 2305, 3024
MvnI CGCG 2 cut(s) 2017, 2480
NciI CCSGG 3 cut(s) 260, 2195, 3052
NdeI CATATG 1 cut(s) 1408
NheI GCTAGC 1 cut(s) 1186
NlaIV GGNNCC 6 cut(s) 590, 627, 751, 834, 1925, 2173
NmuCI GTSAC 1 cut(s) 404
NsiI ATGCAT 4 cut(s) 193, 724, 1457, 2059
NspI RCATGY 5 cut(s) 191, 286, 1654, 2260, 2426
PaeI GCATGC 2 cut(s) 191, 286
PagI TCATGA 1 cut(s) 559
PaqCI CACCTGC 1 cut(s) 1301
PasI CCCWGGG 1 cut(s) 1560
PciI ACATGT 2 cut(s) 1650, 2422
PctI GAATGC 3 cut(s) 191, 1554, 2672
PdmI GAANNNNTTC 3 cut(s) 582, 668, 2170
PfeI GAWTC 8 cut(s) 101, 334, 1513, 1595, 1846, 2540, 2842, 3010
PflFI GACNNNGTC 1 cut(s) 2195
PflMI CCANNNNNTGG 2 cut(s) 2310, 2356
PfoI TCCNGGA 2 cut(s) 1269, 3022
PkrI GCNGC 9 cut(s) 679, 682, 1132, 1661, 2086, 2302, 2482, 2556, 2965
PleI GAGTC 2 cut(s) 2147, 2525
PpsI GAGTC 2 cut(s) 2147, 2525
PscI ACATGT 2 cut(s) 1650, 2422
Psp124BI GAGCTC 1 cut(s) 384
Psp6I CCWGG 6 cut(s) 124, 1269, 1559, 2247, 2303, 3022
PspGI CCWGG 6 cut(s) 124, 1269, 1559, 2247, 2303, 3022
PspN4I GGNNCC 6 cut(s) 590, 627, 751, 834, 1925, 2173
PspPI GGNCC 3 cut(s) 588, 2475, 2984
PstI CTGCAG 1 cut(s) 1681
PsuI RGATCY 3 cut(s) 1150, 1834, 2494
PsyI GACNNNGTC 1 cut(s) 2195
RseI CAYNNNNRTG 3 cut(s) 281, 434, 611
SacI GAGCTC 1 cut(s) 384
SacII CCGCGG 1 cut(s) 2481
SatI GCNGC 9 cut(s) 678, 681, 1131, 1660, 2085, 2301, 2481, 2555, 2964
Sau96I GGNCC 3 cut(s) 588, 2475, 2984
ScaI AGTACT 1 cut(s) 824
SchI GAGTC 2 cut(s) 2147, 2525
ScrFI CCNGG 9 cut(s) 126, 260, 1271, 1561, 2195, 2249, 2305, 3024, 3052
SduI GDGCHC 2 cut(s) 384, 1926
SfaNI GCATC 9 cut(s) 187, 982, 1306, 1410, 1904, 1920, 1999, 2044, 2476
SfcI CTRYAG 1 cut(s) 1677
Sfr303I CCGCGG 1 cut(s) 2481
SgrBI CCGCGG 1 cut(s) 2481
SinI GGWCC 1 cut(s) 2984
SmiMI CAYNNNNRTG 3 cut(s) 281, 434, 611
SmlI CTYRAG 3 cut(s) 383, 1817, 2671
SmoI CTYRAG 3 cut(s) 383, 1817, 2671
SpeI ACTAGT 1 cut(s) 977
SphI GCATGC 2 cut(s) 191, 286
SsiI CCGC 5 cut(s) 681, 2017, 2085, 2478, 2480
SspI AATATT 2 cut(s) 1799, 2218
SstI GAGCTC 1 cut(s) 384
StyD4I CCNGG 9 cut(s) 124, 258, 1269, 1559, 2193, 2247, 2303, 3022, 3050
StyI CCWWGG 1 cut(s) 1014
TaaI ACNGT 2 cut(s) 946, 3040
TaiI ACGT 1 cut(s) 2098
TaqI TCGA 5 cut(s) 9, 203, 341, 1531, 1581
TaqII GACCGA 1 cut(s) 1715
TatI WGTACW 3 cut(s) 597, 822, 1492
TauI GCSGC 3 cut(s) 683, 2087, 2483
TfiI GAWTC 8 cut(s) 101, 334, 1513, 1595, 1846, 2540, 2842, 3010
TscAI CASTG 4 cut(s) 283, 378, 1523, 1901
TseFI GTSAC 1 cut(s) 404
TseI GCWGC 6 cut(s) 677, 1130, 1659, 2300, 2554, 2963
Tsp45I GTSAC 1 cut(s) 404
TspGWI ACGGA 2 cut(s) 503, 2667
TspRI CASTG 4 cut(s) 283, 378, 1523, 1901
Tth111I GACNNNGTC 1 cut(s) 2195
Van91I CCANNNNNTGG 2 cut(s) 2310, 2356
Vha464I CTTAAG 1 cut(s) 2671
VpaK11BI GGWCC 1 cut(s) 2984
XagI CCTNNNNNAGG 1 cut(s) 123
XapI RAATTY 5 cut(s) 1107, 1250, 1459, 1966, 2801
XceI RCATGY 5 cut(s) 191, 286, 1654, 2260, 2426
XcmI CCANNNNNNNNNTGG 1 cut(s) 610
XmnI GAANNNNTTC 3 cut(s) 582, 668, 2170
ZrmI AGTACT 1 cut(s) 824
Zsp2I ATGCAT 4 cut(s) 193, 724, 1457, 2059
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.