Rroxscaffold_4G00315860

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000598
Physical Location & Seq
Reverse (-)
40421965 .. 40426932
4968 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00315860.1

Sequence Viewer

Length: 3336 bp
ATGAGAATAAAGTGGACGACAAAGCTCCACTGTCGCAGTTTGTTTAAAAGGTACTTTTTTAGTAGTACTGAGATGGCGGAGGAAGGCGGCAAGAGCTTTGCCAGGAGAGACCGTCTCCTAGAGATTGAGGCAAAGGCTCGAAACTGGTGGGAAGACAAACAAGTTTTCAGGGCCCAGTCTCGTGCAGAGCCGCCCGGGCCAGGGGAGAAGTTCTTTGGAAACTTCCCCTTCCCTTATATGAACGGTTTTCTGCATCTTGGACATGCCTTCTCACTGTCGAAGCTGGAGTTTGCTGCAGCCTATCATCGATTGAGAGGCGCAAATGTCCTCTTGCCATTTGCTTTTCACTGTACTGGCATGCCCATCAAAGCCTCTGCCGATAAGCTTATTAGGGAGATTCAACAGTTTGGAGAACCTCCTGTTTTTCCCAAAAAAGTGGAGAAGGGGAACGAGGAAGTGGAAGTGGAAGTGGAAGCAGAGGATGTCAATGCAGGCGAACCTCCAGCCAAGCATAAGGGAAAGAAGTCGAAGGCTGCATCAAAATCAAGCGGCGAAGTGTACCAATGGGAAATTATGCAGAGCTTTGGCCTCTCCGACAGTGACATATCCAAATTTCAGGACCCTTATAAGTGGTTGACTTATTTTCCCCCATTGGCTGTGGAGGACCTCAAGGCTTTTGGCCTGGGCTGTGACTGGAGACGTTCCTTTATTACTACAGACATGAACCCTTTTTTCGACGGCTTTGTGAGGTGGCAGGTTAGGAAACTGAAATCCATGGGTAAGATTGTCAAAGATCTCCGTTACACAATATACTCTCCGATGGATGGGCAACCCTGTGCAGATCATGATAGGTCCAGTGGTGAAGGAGTTCAGCCTCAAGAATATACTGTCATCAAAATGGAACTGATGGCACCTTTTCCTTCTAAATTGGGAGTGTTGGAGGGGAGGAAAGTGTTCCTTGCTGCAGCAACACTGAGACCTGAGACCATGTATGGACAAACAAATGCATGGGTATTACCTGATGGTAAGTATGGTGCATATGAAATCAATGAAACAGATGTGTTCATTCTTACACAGAGAGCAGCACGTAATCTTGCTTATCAAAAGTACTCTAGGGTCCCAGACAAACCTACTTGCTTGGTTGAACTGACTGGTTACGATTTGATTGGCCTTCCATTGAAGTCGCCACTAGCAGTGAATCAGATTATTTATGCTCTTCCTATGTTGACTGTCCTAACAGACAAAGGTACTGGGATTGTGACCAGTGTACCTACTGATTCTCCGGATGACTATATGGCCTTGCATGACTTGAAATCAAAACCTGCTTTTAGGGCAAAATTTGGTGTGAAAGATGAGTGGGTACTGCCCTTTGAGATTATCCCAATCATCGATATTCCAGGATATGGAAATAAGGCTGCGGAAAAGGTCTGTGCAGATCTCAAGATCAAAAGCCAGAATGAGAAAGAGAAGCTAGCAGAAGCCAAGAGGTTGACATACTTGAAAGGATTCAATGAAGGAACACTGATTGTGGGGGAATTTAAGGGAAGGAAAGTCCAGGAGGTTAAACCCTTGATAAGGAGCAAGCTTATAGAGGCGGGGGAGGCAATATCATATAGCGAACCTGAGAAGCGGGTACTATCACGATCAGGTGATGAATGTGTTGTGGCGCTTACAGATCAATGGTACATCACTTACGGGGAAACTGAATGGAAAAAACTGGCTGAGGAGTGCTTGTCCGGCATGAATTTGTATTCTGATGAGACACGGCATGGATTTGAACACACATTGAGCTGGTTGAATCAGTGGGCTTGTTCACGATCTTTTGGTCTTGGGACTCGCATCCCCTGGGATGAAGAATTCTTAGTTGAGTCATTGTCTGATTCAACTATTTACATGGCTTATTACACCATTGCTCACTTCCTACACAATGGAGACATGTATGGTTCCAGCAAATCTGCAATTAAACCTGAACAAATGACTGATGAGGTCTGGGATTTTATTTTCTGTGATGGCCCATATCCTAAATCATCCGACATCTCATCATCAATTCTGAGTGAGATGAAGCAGGAGTTTGAATATTGGTATCCTTTTGATCTTCGGGTGTCTGGCAAGGATCTAATACAAAATCATTTAACCTTCTGCATCTACAACCATACAGCTATTATGTCCAAGAAGCACTGGCCTCGTGGTTTCAGATGCAATGGGCACATAATGCTCAATGCTCAGAAAATGTCCAAGTCCACAGGAAACTTTAGGACAATTCGCCAGGCAATTGTGGAATTTTCTGCTGATGCCACTCGCTTTTCTTTGGCTGATGCTGGAGATGGTGTTGATGATGCAAATTTTGCATTTGACACTGCCAATGCTGCAATCCTGCGGCTCACTAAAGAGATCTCATGGATGGAAGAAGTTTTAGGTGCAAACTCTTCTTTGAGGCCAGGACCCCCAACTACTTATGCTGACAGGGTCTTTGCGAATGAGATAAATATTGCTGTGAACAGGACTGAGCAGAATTACAACGATTACATGTTCCGAGAAGCCCTTAAGACTGGATTTTATGATCTTCAGGCTGCTAGGGATGAGTATCGGTTTTCATGTGGTGCTGGGGGAATGAATCGTGATTTGGTGTGGCGTTTTATGGATGTGCAGACACGTCTTATTACTCCAATCTGCCCACACTACGGAGAATACGTATGGAGGGAACTTTTGAAGAAGGATGGCTTTGTGGTAAACGCAGGATGGCCTTTGGCTGACGCTCCGGACCTAACCCTCCAGAGTGCCAATAAGTATTTGCAGGACTCAATCATTTCAATGAGGAAGCTCCTCCAAAAGCAAGTTTCAAGCTCAAAGAAGGGTAAGAAGGGTACTCCAGTCACAGCGTTGACAGAAGACAACAATCTGACCGGCTTAATCTATGTGAACGAGCACTTTGATGGATGGAAGGCAGAGTGCCTGAAAATCCTCCAAATTAACTTTAACAGAGACGGTCGTACTTTTTCTTCAGACAAGGTGATACAAGAAGAGTTGCAAAAAAGTTCTATTGGTCAGTCGAAAGATTTTAGACAAATCCAAAAGCTTTGTATGCCTTTCATGAAGTTCAAGAAGGATGAGGCAATTGCAATTGGGGCTCAGGCCTTGGATTTGAAGCTACCTTTTGGCGAGATTGAGGTCCTTCAAGAGAACTTGGACTTAATTAAGAGACAACTTGGTCTTCAAGAGGTAGAAATATTGTCTGCTACAAATCCTGATGCACTTTGTAAAGCCGGTTCTCTTGTCAAACTGTTAGAGCAGAATCCTCCAGCACCCGGAAGCCCTACTGCCATCTTCTTGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

1111

Amino Acids

125.93

Weight (kDa)

6.58

Isoelectric Point (pI)

43.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1 PF00133 48 - 130 5.5e-08 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1 PF00133 219 - 781 8.7e-16 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g PF09334 689 - 787 1.4e-07 tRNA synthetases class I (M)
tRNA-synt_1e PF01406 695 - 771 3e-08 tRNA synthetases class I (C) catalytic domain
Anticodon_1 PF08264 821 - 945 3.8e-13 Anticodon-binding domain of tRNA ligase
RBD_LARS1 PF24810 974 - 1050 5.8e-07 Leucine--tRNA ligase, RagD-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000598)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09620
fragaria_vesca FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_3g34581 FvH4_4g33720 FvH4_4g33720
malus_domestica MD08G1244600.v1.1 MD15G1439400.v1.1
prunus_persica Prupe.1G582000_v2.0.a1 Prupe.2G015100_v2.0.a1 Prupe.2G123100_v2.0.a1
pyrus_communis pycom08g21210 pycom08g21220 pycom15g38730
rosa_chinensis RchiOBHm_Chr1g0336121 RchiOBHm_Chr1g0353811 RchiOBHm_Chr1g0373331 RchiOBHm_Chr4g0443191 RchiOBHm_Chr4g0443201 RchiOBHm_Chr4g0443211
rosa_laevigata RLG00000003122 RLG00000005948 RLG00000026826 RLG00000028251 RLG00000028252 RLG00000028253 RLG00000029377
rosa_multiflora Rmu_co8002498.1_g000001 Rmu_co8262279.1_g000001 Rmu_co8314139.1_g000001 Rmu_co8393711.1_g000001 Rmu_co8419167.1_g000001 Rmu_sc0007968.1_g000004 Rmu_sc0018919.1_g000004 Rmu_sc0027447.1_g000001
rosa_roxburghii Rroxscaffold_4G00284260 Rroxscaffold_4G00301490 Rroxscaffold_4G00315860 Rroxscaffold_5G00383790 Rroxscaffold_5G00383800
rosa_rugosa Rorug01G0123100 Rorug01G0238700 Rorug01G0377100 Rorug01G0377100 Rorug01G0377200 Rorug01G0377300 Rorug04G0344700 Rorug05G0347900.1
rosa_samantha Rh1AG143800 Rh1AG250300 Rh1AG386300 Rh1BG113400 Rh1BG220800 Rh1BG220900 Rh1BG350400 Rh1CG135500 Rh1CG233100 Rh1CG363500 Rh1DG150200 Rh1DG247700 Rh1DG247800 Rh1DG381300 Rh4AG399400 Rh4AG399500 Rh4BG411000 Rh4BG411100 Rh4BG411200 Rh4CG426500 Rh4CG426600 Rh4DG406100 Rh4DG406200 Rh4DG406300
rosa_wichuraiana Rw0G015190 Rw1G021850 Rw1G034870 Rw4G034440 Rw4G034450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 627
AasI GACNNNNNNGTC 1 cut(s) 3207
Acc36I ACCTGC 2 cut(s) 745, 1330
AccB1I GGYRCC 1 cut(s) 910
AccB7I CCANNNNNTGG 1 cut(s) 1403
AccIII TCCGGA 2 cut(s) 1282, 2758
AciI CCGC 8 cut(s) 77, 87, 191, 549, 1418, 1595, 1630, 2377
AclWI GGATC 1 cut(s) 2121
AcsI RAATTY 7 cut(s) 611, 1337, 1535, 1744, 1856, 2279, 2341
AcuI CTGAAG 2 cut(s) 2549, 2985
AfiI CCNNNNNNNGG 7 cut(s) 200, 201, 824, 1403, 1575, 2681, 3305
AflII CTTAAG 1 cut(s) 2543
AflIII ACRYGT 3 cut(s) 1935, 2526, 2651
AjiI CACGTC 1 cut(s) 2654
AjnI CCWGG 8 cut(s) 101, 199, 681, 1396, 1554, 1844, 2265, 2437
AjuI GAANNNNNNNTTGG 2 cut(s) 2606, 2638
Alw21I GWGCWC 1 cut(s) 2928
AlwI GGATC 1 cut(s) 2121
Ama87I CYCGRG 1 cut(s) 194
Aor13HI TCCGGA 2 cut(s) 1282, 2758
ApaI GGGCCC 1 cut(s) 175
ApeKI GCWGC 9 cut(s) 293, 296, 533, 962, 965, 1082, 1415, 2366, 2570
ApoI RAATTY 7 cut(s) 611, 1337, 1535, 1744, 1856, 2279, 2341
ArsI GACNNNNNNTTYG 2 cut(s) 2453, 2485
Asp700I GAANNNNTTC 3 cut(s) 867, 953, 1505
AspLEI GCGC 2 cut(s) 320, 1669
AsuC2I CCSGG 3 cut(s) 195, 196, 3306
AsuHPI GGTGA 3 cut(s) 872, 1661, 3022
AsuNHI GCTAGC 1 cut(s) 1471
AvaI CYCGRG 1 cut(s) 194
AvaII GGWCC 7 cut(s) 619, 664, 852, 1117, 2441, 2761, 3169
BaeGI GKGCMC 2 cut(s) 175, 2208
BaeI ACNNNNGTAYC 4 cut(s) 1251, 1284, 2856, 2889
BanI GGYRCC 1 cut(s) 910
BanII GRGCYC 2 cut(s) 175, 3130
BarI GAAGNNNNNNTAC 2 cut(s) 2983, 3015
BauI CACGAG 2 cut(s) 180, 2184
BbsI GAAGAC 3 cut(s) 159, 2895, 3203
Bbv12I GWGCWC 1 cut(s) 2928
BbvCI CCTCAGC 1 cut(s) 1722
BbvI GCAGC 9 cut(s) 280, 308, 520, 949, 977, 1094, 1402, 2353, 2557
BceAI ACGGC 2 cut(s) 754, 1781
BcgI CGANNNNNNTGC 2 cut(s) 2165, 2199
BciT130I CCWGG 8 cut(s) 103, 201, 683, 1398, 1556, 1846, 2267, 2439
BciVI GTATCC 1 cut(s) 2094
BcnI CCSGG 3 cut(s) 195, 196, 3306
BfaI CTAG 5 cut(s) 119, 1113, 1190, 1472, 2574
BfmI CTRYAG 3 cut(s) 294, 714, 963
BfoI RGCGCY 1 cut(s) 1670
BfrI CTTAAG 1 cut(s) 2543
BfuAI ACCTGC 2 cut(s) 745, 1330
BfuI GTATCC 1 cut(s) 2094
BglI GCCNNNNNGGC 1 cut(s) 196
BglII AGATCT 3 cut(s) 793, 1435, 2391
BmcAI AGTACT 2 cut(s) 67, 1109
Bme18I GGWCC 7 cut(s) 619, 664, 852, 1117, 2441, 2761, 3169
BmeT110I CYCGRG 1 cut(s) 194
BmgBI CACGTC 1 cut(s) 2654
BmiI GGNNCC 7 cut(s) 173, 621, 912, 1118, 1119, 1945, 2443
BmrI ACTGGG 2 cut(s) 169, 1260
BmsI GCATC 9 cut(s) 262, 545, 1848, 2151, 2186, 2281, 2305, 2326, 3238
BmtI GCTAGC 1 cut(s) 1475
BmuI ACTGGG 2 cut(s) 169, 1260
BpiI GAAGAC 3 cut(s) 159, 2895, 3203
BplI GAGNNNNNCTC 2 cut(s) 99, 131
BpmI CTGGAG 7 cut(s) 305, 486, 715, 2340, 2756, 2853, 3282
Bpu10I CCTNAGC 2 cut(s) 1722, 3129
BpuEI CTTGAG 3 cut(s) 653, 861, 1424
BpuMI CCSGG 3 cut(s) 195, 196, 3306
Bsa29I ATCGAT 2 cut(s) 307, 1389
BsaAI YACGTR 2 cut(s) 1088, 2692
BsaBI GATNNNNATC 1 cut(s) 2583
BsaI GGTCTC 3 cut(s) 102, 970, 977
BsaJI CCNNGG 7 cut(s) 194, 200, 682, 774, 1844, 1845, 3135
BsaWI WCCGGW 2 cut(s) 1282, 2758
Bsc4I CCNNNNNNNGG 7 cut(s) 200, 201, 824, 1403, 1575, 2681, 3305
Bse118I RCCGGY 2 cut(s) 2903, 3263
Bse3DI GCAATG 2 cut(s) 1908, 2206
Bse8I GATNNNNATC 1 cut(s) 2583
BseAI TCCGGA 2 cut(s) 1282, 2758
BseBI CCWGG 8 cut(s) 103, 201, 683, 1398, 1556, 1846, 2267, 2439
BseCI ATCGAT 2 cut(s) 307, 1389
BseDI CCNNGG 7 cut(s) 194, 200, 682, 774, 1844, 1845, 3135
BseJI GATNNNNATC 1 cut(s) 2583
BseLI CCNNNNNNNGG 7 cut(s) 200, 201, 824, 1403, 1575, 2681, 3305
BseMI GCAATG 2 cut(s) 1908, 2206
BseMII CTCAG 9 cut(s) 60, 965, 972, 1614, 1713, 2042, 2237, 2496, 3143
BseRI GAGGAG 2 cut(s) 1739, 2813
BseSI GKGCMC 2 cut(s) 175, 2208
BseXI GCAGC 9 cut(s) 280, 308, 520, 949, 977, 1094, 1402, 2353, 2557
BseYI CCCAGC 1 cut(s) 2603
BsgI GTGCAG 4 cut(s) 204, 858, 1452, 2666
Bsh1285I CGRYCG 1 cut(s) 2989
BshNI GGYRCC 1 cut(s) 910
BshVI ATCGAT 2 cut(s) 307, 1389
BsiEI CGRYCG 1 cut(s) 2989
BsiHKAI GWGCWC 1 cut(s) 2928
BsiHKCI CYCGRG 1 cut(s) 194
BsiSI CCGG 7 cut(s) 195, 1283, 1737, 2759, 2904, 3264, 3306
BslFI GGGAC 2 cut(s) 1103, 1846
BslI CCNNNNNNNGG 7 cut(s) 200, 201, 824, 1403, 1575, 2681, 3305
BsmBI CGTCTC 3 cut(s) 119, 691, 2976
BsmFI GGGAC 2 cut(s) 1103, 1846
Bso31I GGTCTC 3 cut(s) 102, 970, 977
BsoBI CYCGRG 1 cut(s) 194
Bsp120I GGGCCC 1 cut(s) 171
Bsp1286I GDGCHC 4 cut(s) 175, 2208, 2928, 3130
Bsp13I TCCGGA 2 cut(s) 1282, 2758
Bsp19I CCATGG 1 cut(s) 774
BspACI CCGC 8 cut(s) 77, 87, 191, 549, 1418, 1595, 1630, 2377
BspCNI CTCAG 9 cut(s) 61, 966, 973, 1615, 1714, 2043, 2236, 2497, 3142
BspDI ATCGAT 2 cut(s) 307, 1389
BspEI TCCGGA 2 cut(s) 1282, 2758
BspHI TCATGA 2 cut(s) 844, 3090
BspLI GGNNCC 7 cut(s) 173, 621, 912, 1118, 1119, 1945, 2443
BspMAI CTGCAG 2 cut(s) 298, 967
BspMI ACCTGC 2 cut(s) 745, 1330
BspOI GCTAGC 1 cut(s) 1475
BspPI GGATC 1 cut(s) 2121
BspQI GCTCTTC 1 cut(s) 1221
BspT107I GGYRCC 1 cut(s) 910
BspTI CTTAAG 1 cut(s) 2543
BspTNI GGTCTC 3 cut(s) 102, 970, 977
BsrDI GCAATG 2 cut(s) 1908, 2206
BsrFI RCCGGY 2 cut(s) 2903, 3263
BssAI RCCGGY 2 cut(s) 2903, 3263
BssECI CCNNGG 7 cut(s) 194, 200, 682, 774, 1844, 1845, 3135
BssSI CACGAG 2 cut(s) 180, 2184
BssT1I CCWWGG 2 cut(s) 774, 3135
Bst2BI CACGAG 2 cut(s) 180, 2184
Bst2UI CCWGG 8 cut(s) 103, 201, 683, 1398, 1556, 1846, 2267, 2439
Bst6I CTCTTC 3 cut(s) 1221, 2431, 3015
BstAFI CTTAAG 1 cut(s) 2543
BstAPI GCANNNNNTGC 2 cut(s) 2212, 2345
BstBAI YACGTR 2 cut(s) 1088, 2692
BstC8I GCNNGC 4 cut(s) 359, 493, 1473, 1583
BstDSI CCRYGG 1 cut(s) 774
BstENI CCTNNNNNAGG 1 cut(s) 1573
BstH2I RGCGCY 1 cut(s) 1670
BstHHI GCGC 2 cut(s) 320, 1669
BstMCI CGRYCG 1 cut(s) 2989
BstNI CCWGG 8 cut(s) 103, 201, 683, 1398, 1556, 1846, 2267, 2439
BstNSI RCATGY 4 cut(s) 266, 361, 1939, 2530
BstSFI CTRYAG 3 cut(s) 294, 714, 963
BstSLI GKGCMC 2 cut(s) 175, 2208
BstSNI TACGTA 1 cut(s) 2692
BstV1I GCAGC 9 cut(s) 280, 308, 520, 949, 977, 1094, 1402, 2353, 2557
BstV2I GAAGAC 3 cut(s) 159, 2895, 3203
BstX2I RGATCY 4 cut(s) 793, 1435, 2113, 2391
BstXI CCANNNNNNTGG 1 cut(s) 436
BstYI RGATCY 4 cut(s) 793, 1435, 2113, 2391
Bsu15I ATCGAT 2 cut(s) 307, 1389
BsuI GTATCC 1 cut(s) 2094
BsuTUI ATCGAT 2 cut(s) 307, 1389
BtgI CCRYGG 1 cut(s) 774
BtrI CACGTC 1 cut(s) 2654
BtsI GCAGTG 2 cut(s) 1200, 2355
BveI ACCTGC 2 cut(s) 745, 1330
Cac8I GCNNGC 4 cut(s) 359, 493, 1473, 1583
CciI TCATGA 2 cut(s) 844, 3090
CfoI GCGC 2 cut(s) 320, 1669
Cfr10I RCCGGY 2 cut(s) 2903, 3263
Cfr9I CCCGGG 1 cut(s) 194
ClaI ATCGAT 2 cut(s) 307, 1389
CseI GACGC 1 cut(s) 2762
DraI TTTAAA 1 cut(s) 46
DrdI GACNNNNNNGTC 1 cut(s) 3207
DseDI GACNNNNNNGTC 1 cut(s) 3207
Eam1104I CTCTTC 3 cut(s) 1221, 2431, 3015
EarI CTCTTC 3 cut(s) 1221, 2431, 3015
EciI GGCGGA 1 cut(s) 92
Eco105I TACGTA 1 cut(s) 2692
Eco130I CCWWGG 2 cut(s) 774, 3135
Eco147I AGGCCT 1 cut(s) 3134
Eco24I GRGCYC 2 cut(s) 175, 3130
Eco31I GGTCTC 3 cut(s) 102, 970, 977
Eco47I GGWCC 7 cut(s) 619, 664, 852, 1117, 2441, 2761, 3169
Eco57I CTGAAG 2 cut(s) 2549, 2985
Eco88I CYCGRG 1 cut(s) 194
EcoNI CCTNNNNNAGG 1 cut(s) 1573
EcoO109I RGGNCCY 6 cut(s) 171, 619, 664, 1117, 2441, 3169
EcoRI GAATTC 1 cut(s) 1856
EcoRII CCWGG 8 cut(s) 101, 199, 681, 1396, 1554, 1844, 2265, 2437
EcoT14I CCWWGG 2 cut(s) 774, 3135
EcoT22I ATGCAT 1 cut(s) 1009
EcoT38I GRGCYC 2 cut(s) 175, 3130
ErhI CCWWGG 2 cut(s) 774, 3135
Esp3I CGTCTC 3 cut(s) 119, 691, 2976
FalI AAGNNNNNCTT 4 cut(s) 942, 974, 2705, 2737
FaqI GGGAC 2 cut(s) 1103, 1846
FauI CCCGC 2 cut(s) 1588, 1623
FauNDI CATATG 1 cut(s) 1039
FriOI GRGCYC 2 cut(s) 175, 3130
FspBI CTAG 5 cut(s) 119, 1113, 1190, 1472, 2574
GlaI GCGC 2 cut(s) 319, 1668
GsaI CCCAGC 1 cut(s) 2607
GsuI CTGGAG 7 cut(s) 305, 486, 715, 2340, 2756, 2853, 3282
HaeII RGCGCY 1 cut(s) 1670
HapII CCGG 7 cut(s) 195, 1283, 1737, 2759, 2904, 3264, 3306
HgaI GACGC 1 cut(s) 2762
HhaI GCGC 2 cut(s) 320, 1669
Hin6I GCGC 2 cut(s) 318, 1667
HinP1I GCGC 2 cut(s) 318, 1667
HincII GTYRAC 4 cut(s) 636, 1227, 1491, 2883
HindII GTYRAC 4 cut(s) 636, 1227, 1491, 2883
HindIII AAGCTT 3 cut(s) 383, 1583, 3074
HpaII CCGG 7 cut(s) 195, 1283, 1737, 2759, 2904, 3264, 3306
HphI GGTGA 3 cut(s) 872, 1661, 3022
Hpy99I CGWCG 1 cut(s) 740
HpyCH4IV ACGT 4 cut(s) 700, 1087, 2653, 2691
HpySE526I ACGT 4 cut(s) 700, 1087, 2653, 2691
HspAI GCGC 2 cut(s) 318, 1667
KflI GGGWCCC 1 cut(s) 1117
Kpn2I TCCGGA 2 cut(s) 1282, 2758
LguI GCTCTTC 1 cut(s) 1221
LmnI GCTCC 4 cut(s) 30, 1578, 2761, 2826
Lsp1109I GCAGC 9 cut(s) 280, 308, 520, 949, 977, 1094, 1402, 2353, 2557
LweI GCATC 9 cut(s) 262, 545, 1848, 2151, 2186, 2281, 2305, 2326, 3238
MaeI CTAG 5 cut(s) 119, 1113, 1190, 1472, 2574
MaeII ACGT 4 cut(s) 700, 1087, 2653, 2691
MaeIII GTNAC 6 cut(s) 599, 689, 800, 1154, 1258, 2872
MfeI CAATTG 3 cut(s) 2271, 3114, 3120
MflI RGATCY 4 cut(s) 793, 1435, 2113, 2391
MhlI GDGCHC 4 cut(s) 175, 2208, 2928, 3130
MlyI GAGTC 3 cut(s) 1828, 1877, 2792
MmeI TCCRAC 3 cut(s) 618, 918, 2055
Mph1103I ATGCAT 1 cut(s) 1009
MroI TCCGGA 2 cut(s) 1282, 2758
MroXI GAANNNNTTC 3 cut(s) 867, 953, 1505
MslI CAYNNNNRTG 3 cut(s) 896, 2810, 2931
MspCI CTTAAG 1 cut(s) 2543
MspI CCGG 7 cut(s) 195, 1283, 1737, 2759, 2904, 3264, 3306
MunI CAATTG 3 cut(s) 2271, 3114, 3120
MvaI CCWGG 8 cut(s) 103, 201, 683, 1398, 1556, 1846, 2267, 2439
NciI CCSGG 3 cut(s) 195, 196, 3306
NcoI CCATGG 1 cut(s) 774
NdeI CATATG 1 cut(s) 1039
NheI GCTAGC 1 cut(s) 1471
NlaIV GGNNCC 7 cut(s) 173, 621, 912, 1118, 1119, 1945, 2443
NmuCI GTSAC 4 cut(s) 599, 689, 1258, 2872
NsiI ATGCAT 1 cut(s) 1009
NspI RCATGY 4 cut(s) 266, 361, 1939, 2530
PacI TTAATTAA 1 cut(s) 3195
PaeI GCATGC 1 cut(s) 361
PagI TCATGA 2 cut(s) 844, 3090
PasI CCCWGGG 1 cut(s) 1845
PceI AGGCCT 1 cut(s) 3134
PciI ACATGT 2 cut(s) 1935, 2526
PciSI GCTCTTC 1 cut(s) 1221
PcsI WCGNNNNNNNCGW 1 cut(s) 2688
PdmI GAANNNNTTC 3 cut(s) 867, 953, 1505
PfeI GAWTC 8 cut(s) 397, 1198, 1277, 1506, 1798, 1880, 2614, 3292
PflFI GACNNNGTC 1 cut(s) 2465
PflMI CCANNNNNTGG 1 cut(s) 1403
PfoI TCCNGGA 2 cut(s) 1396, 1554
PleI GAGTC 3 cut(s) 1828, 1876, 2792
PpsI GAGTC 3 cut(s) 1828, 1876, 2792
Ppu21I YACGTR 2 cut(s) 1088, 2692
PpuMI RGGWCCY 5 cut(s) 619, 664, 1117, 2441, 3169
PscI ACATGT 2 cut(s) 1935, 2526
PsiI TTATAA 1 cut(s) 627
Psp5II RGGWCCY 5 cut(s) 619, 664, 1117, 2441, 3169
Psp6I CCWGG 8 cut(s) 101, 199, 681, 1396, 1554, 1844, 2265, 2437
PspFI CCCAGC 1 cut(s) 2603
PspGI CCWGG 8 cut(s) 101, 199, 681, 1396, 1554, 1844, 2265, 2437
PspN4I GGNNCC 7 cut(s) 173, 621, 912, 1118, 1119, 1945, 2443
PspOMI GGGCCC 1 cut(s) 171
PspPPI RGGWCCY 5 cut(s) 619, 664, 1117, 2441, 3169
PsrI GAACNNNNNNTAC 2 cut(s) 3250, 3282
PstI CTGCAG 2 cut(s) 298, 967
PsuI RGATCY 4 cut(s) 793, 1435, 2113, 2391
PsyI GACNNNGTC 1 cut(s) 2465
RseI CAYNNNNRTG 3 cut(s) 896, 2810, 2931
SapI GCTCTTC 1 cut(s) 1221
ScaI AGTACT 2 cut(s) 67, 1109
SchI GAGTC 3 cut(s) 1828, 1877, 2792
SduI GDGCHC 4 cut(s) 175, 2208, 2928, 3130
SfaNI GCATC 9 cut(s) 262, 545, 1848, 2151, 2186, 2281, 2305, 2326, 3238
SfcI CTRYAG 3 cut(s) 294, 714, 963
SinI GGWCC 7 cut(s) 619, 664, 852, 1117, 2441, 2761, 3169
SmaI CCCGGG 1 cut(s) 196
SmiMI CAYNNNNRTG 3 cut(s) 896, 2810, 2931
SmlI CTYRAG 4 cut(s) 668, 876, 1439, 2543
SmoI CTYRAG 4 cut(s) 668, 876, 1439, 2543
SnaBI TACGTA 1 cut(s) 2692
SphI GCATGC 1 cut(s) 361
SrfI GCCCGGGC 1 cut(s) 196
SseBI AGGCCT 1 cut(s) 3134
SsiI CCGC 8 cut(s) 77, 87, 191, 549, 1418, 1595, 1630, 2377
SspI AATATT 3 cut(s) 2078, 2488, 3228
SspMI CTAG 5 cut(s) 119, 1113, 1190, 1472, 2574
StuI AGGCCT 1 cut(s) 3134
StyI CCWWGG 2 cut(s) 774, 3135
TaiI ACGT 4 cut(s) 703, 1090, 2656, 2694
TaqI TCGA 7 cut(s) 139, 278, 307, 527, 735, 1389, 3050
TatI WGTACW 3 cut(s) 65, 350, 1107
TauI GCSGC 4 cut(s) 90, 193, 552, 2380
TfiI GAWTC 8 cut(s) 397, 1198, 1277, 1506, 1798, 1880, 2614, 3292
TseFI GTSAC 4 cut(s) 599, 689, 1258, 2872
TseI GCWGC 9 cut(s) 293, 296, 533, 962, 965, 1082, 1415, 2366, 2570
Tsp45I GTSAC 4 cut(s) 599, 689, 1258, 2872
TspGWI ACGGA 2 cut(s) 788, 2697
TspMI CCCGGG 1 cut(s) 194
Tth111I GACNNNGTC 1 cut(s) 2465
Van91I CCANNNNNTGG 1 cut(s) 1403
Vha464I CTTAAG 1 cut(s) 2543
VpaK11BI GGWCC 7 cut(s) 619, 664, 852, 1117, 2441, 2761, 3169
XagI CCTNNNNNAGG 1 cut(s) 1573
XapI RAATTY 7 cut(s) 611, 1337, 1535, 1744, 1856, 2279, 2341
XceI RCATGY 4 cut(s) 266, 361, 1939, 2530
XmaI CCCGGG 1 cut(s) 194
XmnI GAANNNNTTC 3 cut(s) 867, 953, 1505
XspI CTAG 5 cut(s) 119, 1113, 1190, 1472, 2574
ZrmI AGTACT 2 cut(s) 67, 1109
Zsp2I ATGCAT 1 cut(s) 1009
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.