Rh1BG113400

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000598
Physical Location & Seq
Forward (+)
19326100 .. 19332550
6451 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG113400.1

Sequence Viewer

Length: 3336 bp
ATGAGAGTAAAGTGGACGACAAAGCTCCACTGTCGCAGTTTGTTTAAAAGGTACTTTAGTAGTACTGAGATGGCGGCGGAGGAAGGCGGGAAGAGCTTTGCCAGGAGAGACCGTCTCCTAGAGATTGAGGGAAAGGCTCGAAGCTGGTGGGAAGACGAACAAGTTTTCAGGGCCCAGTCTCATGCAGAGCCGCCCGGGCCAGGGGAGAAGTTCTTTGGGAACTTCCCCTTCCCTTATATGAACGGTATTCTGCATCTCGGACATGCTTTCTCACTGTCGAAGCTGGAGTTTGCTGCAGCCTATCATCGATTGAGAGGCGCAAATGTCCTCTTGCCTTTTGCTTTTCACTGTACTGGCATGCCCATCAAAGCCTCTGCCGATAAGCTTATTAGGGAGATTCAACAGTTTGGAGAACCTCCTGTTTTTCCCAAAAAAGTGGAGAAAGGGAACGAGGAAGTGGAAGTGGAAGCAGAGGATGTCAATGCAGGTGAACCTCCAGCCAAGTATAAGGGAAAGAAGTCGAAGGCTGCATCAAAATCAAGCGGCGAAGTGTACCAATGGGAAATTATGCAGAGCTTTGGCCTCTCCGACAGTGACATATCCAAATTTCAGGACCCTTATAAGTGGTTGACTTATTTTCCCCCATTGGCAGTGGAGGACCTCAAGGCTTTTGGCCTGGGCTGTGACTGGAGACGTTCCTTTATTACTACAGACATGAACCCTTTTTTCGACGCCTTTGTGAGGTGGCAGGTTAGGAAACTGAAATCCATGGGTAAGATTGTCAAAGATCTCCGTTACACGATATACTCTCCGATGGATGGGCAACCCTGTGCAGATCATGATAGGTCCAGTGGTGAAGGAGTTCAGCCCCAAGAATATACTGTCATCAAAATGGAACTGATGGCACCTTTTCCTTCTAAATTGGGAGTGTTGGAGGGGAGGAAAGTGTTCCTTGCTGCAGCAACACTGAGACCTGAGACCATGTATGGACAAACAAATGCATGGGTATTGCCTGATGGTAAGTATGGTGCATATGAAATCAATGAAACAGATGTGTTCATTCTTACACAGAGAGCAGCACGTAATCTTGCTTATCAAAAGTACTCTAGGGTCCCAGAGAAACTTACTTGCTTGGTTGAACTGACTGGTTACGATTTGATTGGCCTTCCATTGAAGTCGCCACTAGCAGTGAATCAGATTATTTATGCTCTTCCTATGTTGACTGTCCTAACAGACAAAGGTACTGGGATTGTGACCAGTGTACCTACTGATTCTCCGGATGACTATATGGCCTTGCATGACTTGAAATCAAAACCTGCTTTTAGGGCAAAATATGGTGTGAAAGATGAGTGGGTACTGCCCTTTGAGATTATCCCAATCATTGATATTCCAGGATATGGAAATAAGGCTGCAGAAAAGGTCTGTGCAGATCTCAAGATCAAAAGCCAGAATGAGAAAGAGAAGCTAGCAGAAGCCAAGAGGTTGACATACTTGAAAGGATTCAATGAAGGAACACTGATTGTGGGGGAATTTAAGGGAAGGAAAGTCCAGGAGGTTAAACCCTTGATAAGGAGCAAGCTTATAGAGGCGGGAGAGGCAATATCATACAGTGAACCTGAGAAGCGGGTACTATCACGATCAGGTGATGAATGTGTTGTGGCGCTTACAGATCAATGGTACATCACTTACGGGGAAACTGAATGGAAAAAACTGGCTGAGGAGTGCTTGTCCGGCATGAGTTTGTATTCTGATGAGACACGGCATGGATTTGAACACACATTGAGCTGGTTGAATCAGTGGGCTTGTTCACGATCTTTTGGTCTTGGGACTCGCATCCCCTGGGATGAAGAATTCTTAGTTGAGTCATTGTCTGATTCAACTATTTACATGGCTTATTACACCATTGCTCACTTCCTACACAATGGAGACATGTATGGTTCCAGCAAATCTGCAATTAAACCTGAACAAATGACTGATGAGGTCTGGGATTTTATTTTCTGTGATGGCCCATATCCTAAATCATCCGACATCTCATCATCAATTCTGAGTGAGATGAAGCAGGAGTTTGAATATTGGTATCCTTTTGATCTTCGGGTGTCTGGCAAGGATCTTATACAAAATCATTTAACCTTCTGCATCTACAACCATACAGCTATTATGTCCAAGAAGCACTGGCCCTGTGGTTTCAGATGCAATGGGCACATAATGCTCAATGCTCAGAAAATGTCCAAGTCCACAGGAAACTTTAGGACAATTCGCCAGGCAATTGAGGAATTTTCTGCTGATGCCACTCGCTTTTCTTTGGCTGATGCTGGAGATGGTGTTGATGATGCAAATTTTGCATTTGACACTGCCAATGCTGCAATCCTGCGGCTCACTAAAGAGATCTCATGGATGGAAGAAGTTATAGGTGCAGAATCTTCTTTGAGACCAGGACCCCCAACTACTTATGCTGACAGGGTCTTTGCAAATGAGATAAATATTGCTGTGAACAGGACTGAGCAGAATTACAGTGATTACATGTTCCGAGAAGCCCTCAAGACTGGATTTTATGATCTTCAGGCTGCTAGGGATGAGTATCGGTTTTCATGTGGTGCTGGGGGAATGAATCGTGATTTGTTGTGGCGTTTTATGGATGTGCAGACACGTCTTATTACTCCAATCTGCCCACACTATGGAGAATACGTATGGAGGGAACTTTTGAAGAAGGACGGCTTTGTGGTAAACGCAGGATGGCCTTTGGCTGACGCTCCGGACCTAACCCTCCAGAGTGCCAATAAGTATTTGCAGGACTCAATCATTTCAATGAGGAAGCTCCTCCAAAAGCAAGTTTCAAGCTCAAAGAAGGGTAAGAAGGGTACTCCAGTCACAGCGTTGACAGAAGACAACAATCTGACTGGCTTAATCTATGTGAATGAGCACTTTGATGGATGGAAGGCAGAGTGCCTGAAAATCCTCCAAATTAACTTTAACAGAGACAGTCGTACTTTTTCTTCAGACAAGGTGATACAAGAAGCGTTGCAAAAAAGTTCTATTGGTCAGTCGAAAGATTTTAGACAAATCCAAAAGCTTTGTATGCCTTTCATGAAGTTCAAGAAGGATGAGGCAATTGCAATTGGGGCTCAGGCCTTGGATTTGAAGCTACCTTTTGGCGAGATTGAGGTCCTTCAAGAGAACTTGGACTTAATTAAGAGACAACTTGGTCTTCAAGAGGTAGAAATATTGTCTGCTACAAATCCTGATGCACTTTCTAAAGCCGGTTCTCTTGTCAAACTGTTAGAGCAGAATCCTCCAGCACCCGGAAGCCCTACTGCTATCTTCTTGACCTGTATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

1111

Amino Acids

125.73

Weight (kDa)

6.23

Isoelectric Point (pI)

45.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1 PF00133 48 - 130 4.3e-08 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1 PF00133 217 - 779 3.1e-16 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g PF09334 687 - 785 6.6e-08 tRNA synthetases class I (M)
tRNA-synt_1e PF01406 693 - 769 4.8e-08 tRNA synthetases class I (C) catalytic domain
Anticodon_1 PF08264 819 - 943 2.1e-13 Anticodon-binding domain of tRNA ligase
RBD_LARS1 PF24810 972 - 1048 1.6e-06 Leucine--tRNA ligase, RagD-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000598)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09620
fragaria_vesca FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_3g34581 FvH4_4g33720 FvH4_4g33720
malus_domestica MD08G1244600.v1.1 MD15G1439400.v1.1
prunus_persica Prupe.1G582000_v2.0.a1 Prupe.2G015100_v2.0.a1 Prupe.2G123100_v2.0.a1
pyrus_communis pycom08g21210 pycom08g21220 pycom15g38730
rosa_chinensis RchiOBHm_Chr1g0336121 RchiOBHm_Chr1g0353811 RchiOBHm_Chr1g0373331 RchiOBHm_Chr4g0443191 RchiOBHm_Chr4g0443201 RchiOBHm_Chr4g0443211
rosa_laevigata RLG00000003122 RLG00000005948 RLG00000026826 RLG00000028251 RLG00000028252 RLG00000028253 RLG00000029377
rosa_multiflora Rmu_co8002498.1_g000001 Rmu_co8262279.1_g000001 Rmu_co8314139.1_g000001 Rmu_co8393711.1_g000001 Rmu_co8419167.1_g000001 Rmu_sc0007968.1_g000004 Rmu_sc0018919.1_g000004 Rmu_sc0027447.1_g000001
rosa_roxburghii Rroxscaffold_4G00284260 Rroxscaffold_4G00301490 Rroxscaffold_4G00315860 Rroxscaffold_5G00383790 Rroxscaffold_5G00383800
rosa_rugosa Rorug01G0123100 Rorug01G0238700 Rorug01G0377100 Rorug01G0377100 Rorug01G0377200 Rorug01G0377300 Rorug04G0344700 Rorug05G0347900.1
rosa_samantha Rh1AG143800 Rh1AG250300 Rh1AG386300 Rh1BG113400 Rh1BG220800 Rh1BG220900 Rh1BG350400 Rh1CG135500 Rh1CG233100 Rh1CG363500 Rh1DG150200 Rh1DG247700 Rh1DG247800 Rh1DG381300 Rh4AG399400 Rh4AG399500 Rh4BG411000 Rh4BG411100 Rh4BG411200 Rh4CG426500 Rh4CG426600 Rh4DG406100 Rh4DG406200 Rh4DG406300
rosa_wichuraiana Rw0G015190 Rw1G021850 Rw1G034870 Rw4G034440 Rw4G034450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 621
AarI CACCTGC 1 cut(s) 476
AasI GACNNNNNNGTC 1 cut(s) 3201
Acc36I ACCTGC 3 cut(s) 476, 739, 1324
AccB1I GGYRCC 1 cut(s) 904
AccB7I CCANNNNNTGG 2 cut(s) 1397, 2675
AccIII TCCGGA 2 cut(s) 1276, 2752
AciI CCGC 8 cut(s) 74, 77, 87, 191, 543, 1589, 1624, 2371
AclWI GGATC 1 cut(s) 2115
AcsI RAATTY 5 cut(s) 605, 1529, 1850, 2273, 2335
AcuI CTGAAG 2 cut(s) 2543, 2979
AcyI GRCGYC 1 cut(s) 732
AfiI CCNNNNNNNGG 8 cut(s) 200, 201, 741, 818, 1397, 1569, 2675, 3299
AflIII ACRYGT 3 cut(s) 1929, 2520, 2645
AjiI CACGTC 1 cut(s) 2648
AjnI CCWGG 8 cut(s) 101, 199, 675, 1390, 1548, 1838, 2259, 2431
Alw21I GWGCWC 1 cut(s) 2922
AlwI GGATC 1 cut(s) 2115
Ama87I CYCGRG 1 cut(s) 194
Aor13HI TCCGGA 2 cut(s) 1276, 2752
ApaI GGGCCC 1 cut(s) 175
ApeKI GCWGC 9 cut(s) 293, 296, 527, 956, 959, 1076, 1409, 2360, 2564
ApoI RAATTY 5 cut(s) 605, 1529, 1850, 2273, 2335
ArsI GACNNNNNNTTYG 2 cut(s) 2447, 2479
Asp700I GAANNNNTTC 3 cut(s) 861, 947, 1499
AspLEI GCGC 2 cut(s) 320, 1663
AsuC2I CCSGG 3 cut(s) 195, 196, 3300
AsuHPI GGTGA 4 cut(s) 500, 866, 1655, 3016
AsuNHI GCTAGC 1 cut(s) 1465
AvaI CYCGRG 1 cut(s) 194
AvaII GGWCC 7 cut(s) 613, 658, 846, 1111, 2435, 2755, 3163
BaeGI GKGCMC 2 cut(s) 175, 2202
BaeI ACNNNNGTAYC 6 cut(s) 43, 76, 1245, 1278, 2850, 2883
BanI GGYRCC 1 cut(s) 904
BanII GRGCYC 2 cut(s) 175, 3124
BarI GAAGNNNNNNTAC 2 cut(s) 2977, 3009
BbsI GAAGAC 3 cut(s) 159, 2889, 3197
Bbv12I GWGCWC 1 cut(s) 2922
BbvCI CCTCAGC 1 cut(s) 1716
BbvI GCAGC 9 cut(s) 280, 308, 514, 943, 971, 1088, 1396, 2347, 2551
BceAI ACGGC 2 cut(s) 1775, 2728
BciT130I CCWGG 8 cut(s) 103, 201, 677, 1392, 1550, 1840, 2261, 2433
BciVI GTATCC 1 cut(s) 2088
BcnI CCSGG 3 cut(s) 195, 196, 3300
BfaI CTAG 5 cut(s) 119, 1107, 1184, 1466, 2568
BfmI CTRYAG 4 cut(s) 294, 708, 957, 1410
BfoI RGCGCY 1 cut(s) 1664
BfuAI ACCTGC 3 cut(s) 476, 739, 1324
BfuI GTATCC 1 cut(s) 2088
BglI GCCNNNNNGGC 1 cut(s) 196
BglII AGATCT 3 cut(s) 787, 1429, 2385
BmcAI AGTACT 2 cut(s) 64, 1103
Bme18I GGWCC 7 cut(s) 613, 658, 846, 1111, 2435, 2755, 3163
BmeT110I CYCGRG 1 cut(s) 194
BmgBI CACGTC 1 cut(s) 2648
BmiI GGNNCC 7 cut(s) 173, 615, 906, 1112, 1113, 1939, 2437
BmrI ACTGGG 2 cut(s) 169, 1254
BmsI GCATC 9 cut(s) 262, 539, 1842, 2145, 2180, 2275, 2299, 2320, 3232
BmtI GCTAGC 1 cut(s) 1469
BmuI ACTGGG 2 cut(s) 169, 1254
BpiI GAAGAC 3 cut(s) 159, 2889, 3197
BplI GAGNNNNNCTC 4 cut(s) 99, 131, 2520, 2552
BpmI CTGGAG 7 cut(s) 305, 480, 709, 2334, 2750, 2847, 3276
Bpu10I CCTNAGC 2 cut(s) 1716, 3123
BpuEI CTTGAG 3 cut(s) 647, 1418, 2522
BpuMI CCSGG 3 cut(s) 195, 196, 3300
Bsa29I ATCGAT 1 cut(s) 307
BsaAI YACGTR 2 cut(s) 1082, 2686
BsaBI GATNNNNATC 1 cut(s) 2577
BsaHI GRCGYC 1 cut(s) 732
BsaI GGTCTC 4 cut(s) 102, 964, 971, 2422
BsaJI CCNNGG 7 cut(s) 194, 200, 676, 768, 1838, 1839, 3129
BsaWI WCCGGW 2 cut(s) 1276, 2752
Bsc4I CCNNNNNNNGG 8 cut(s) 200, 201, 741, 818, 1397, 1569, 2675, 3299
Bse118I RCCGGY 1 cut(s) 3257
Bse3DI GCAATG 2 cut(s) 1902, 2200
Bse8I GATNNNNATC 1 cut(s) 2577
BseAI TCCGGA 2 cut(s) 1276, 2752
BseBI CCWGG 8 cut(s) 103, 201, 677, 1392, 1550, 1840, 2261, 2433
BseCI ATCGAT 1 cut(s) 307
BseDI CCNNGG 7 cut(s) 194, 200, 676, 768, 1838, 1839, 3129
BseJI GATNNNNATC 1 cut(s) 2577
BseLI CCNNNNNNNGG 8 cut(s) 200, 201, 741, 818, 1397, 1569, 2675, 3299
BseMI GCAATG 2 cut(s) 1902, 2200
BseMII CTCAG 9 cut(s) 57, 959, 966, 1608, 1707, 2036, 2231, 2490, 3137
BseRI GAGGAG 2 cut(s) 1733, 2807
BseSI GKGCMC 2 cut(s) 175, 2202
BseXI GCAGC 9 cut(s) 280, 308, 514, 943, 971, 1088, 1396, 2347, 2551
BseYI CCCAGC 1 cut(s) 2597
BsgI GTGCAG 4 cut(s) 852, 1446, 2433, 2660
BshNI GGYRCC 1 cut(s) 904
BshVI ATCGAT 1 cut(s) 307
BsiHKAI GWGCWC 1 cut(s) 2922
BsiHKCI CYCGRG 1 cut(s) 194
BsiSI CCGG 6 cut(s) 195, 1277, 1731, 2753, 3258, 3300
BslFI GGGAC 2 cut(s) 1097, 1840
BslI CCNNNNNNNGG 8 cut(s) 200, 201, 741, 818, 1397, 1569, 2675, 3299
BsmBI CGTCTC 2 cut(s) 119, 685
BsmFI GGGAC 2 cut(s) 1097, 1840
Bso31I GGTCTC 4 cut(s) 102, 964, 971, 2422
BsoBI CYCGRG 1 cut(s) 194
Bsp120I GGGCCC 1 cut(s) 171
Bsp1286I GDGCHC 4 cut(s) 175, 2202, 2922, 3124
Bsp13I TCCGGA 2 cut(s) 1276, 2752
Bsp19I CCATGG 1 cut(s) 768
BspACI CCGC 8 cut(s) 74, 77, 87, 191, 543, 1589, 1624, 2371
BspCNI CTCAG 9 cut(s) 58, 960, 967, 1609, 1708, 2037, 2230, 2491, 3136
BspDI ATCGAT 1 cut(s) 307
BspEI TCCGGA 2 cut(s) 1276, 2752
BspHI TCATGA 2 cut(s) 838, 3084
BspLI GGNNCC 7 cut(s) 173, 615, 906, 1112, 1113, 1939, 2437
BspMAI CTGCAG 3 cut(s) 298, 961, 1414
BspMI ACCTGC 3 cut(s) 476, 739, 1324
BspOI GCTAGC 1 cut(s) 1469
BspPI GGATC 1 cut(s) 2115
BspQI GCTCTTC 2 cut(s) 86, 1215
BspT107I GGYRCC 1 cut(s) 904
BspTNI GGTCTC 4 cut(s) 102, 964, 971, 2422
BsrDI GCAATG 2 cut(s) 1902, 2200
BsrFI RCCGGY 1 cut(s) 3257
BssAI RCCGGY 1 cut(s) 3257
BssECI CCNNGG 7 cut(s) 194, 200, 676, 768, 1838, 1839, 3129
BssNI GRCGYC 1 cut(s) 732
BssT1I CCWWGG 2 cut(s) 768, 3129
Bst2UI CCWGG 8 cut(s) 103, 201, 677, 1392, 1550, 1840, 2261, 2433
Bst6I CTCTTC 2 cut(s) 86, 1215
BstACI GRCGYC 1 cut(s) 732
BstAPI GCANNNNNTGC 2 cut(s) 2206, 2339
BstBAI YACGTR 2 cut(s) 1082, 2686
BstC8I GCNNGC 3 cut(s) 359, 1467, 1577
BstDSI CCRYGG 1 cut(s) 768
BstENI CCTNNNNNAGG 2 cut(s) 739, 1567
BstH2I RGCGCY 1 cut(s) 1664
BstHHI GCGC 2 cut(s) 320, 1663
BstNI CCWGG 8 cut(s) 103, 201, 677, 1392, 1550, 1840, 2261, 2433
BstNSI RCATGY 4 cut(s) 266, 361, 1933, 2524
BstSFI CTRYAG 4 cut(s) 294, 708, 957, 1410
BstSLI GKGCMC 2 cut(s) 175, 2202
BstSNI TACGTA 1 cut(s) 2686
BstV1I GCAGC 9 cut(s) 280, 308, 514, 943, 971, 1088, 1396, 2347, 2551
BstV2I GAAGAC 3 cut(s) 159, 2889, 3197
BstX2I RGATCY 4 cut(s) 787, 1429, 2107, 2385
BstXI CCANNNNNNTGG 1 cut(s) 436
BstYI RGATCY 4 cut(s) 787, 1429, 2107, 2385
Bsu15I ATCGAT 1 cut(s) 307
BsuI GTATCC 1 cut(s) 2088
BsuTUI ATCGAT 1 cut(s) 307
BtgI CCRYGG 1 cut(s) 768
BtrI CACGTC 1 cut(s) 2648
BtsI GCAGTG 3 cut(s) 657, 1194, 2349
BveI ACCTGC 3 cut(s) 476, 739, 1324
Cac8I GCNNGC 3 cut(s) 359, 1467, 1577
CciI TCATGA 2 cut(s) 838, 3084
CfoI GCGC 2 cut(s) 320, 1663
Cfr10I RCCGGY 1 cut(s) 3257
Cfr9I CCCGGG 1 cut(s) 194
ClaI ATCGAT 1 cut(s) 307
CseI GACGC 2 cut(s) 740, 2756
DraI TTTAAA 1 cut(s) 46
DrdI GACNNNNNNGTC 1 cut(s) 3201
DseDI GACNNNNNNGTC 1 cut(s) 3201
Eam1104I CTCTTC 2 cut(s) 86, 1215
EarI CTCTTC 2 cut(s) 86, 1215
EciI GGCGGA 1 cut(s) 92
Eco105I TACGTA 1 cut(s) 2686
Eco130I CCWWGG 2 cut(s) 768, 3129
Eco147I AGGCCT 1 cut(s) 3128
Eco24I GRGCYC 2 cut(s) 175, 3124
Eco31I GGTCTC 4 cut(s) 102, 964, 971, 2422
Eco47I GGWCC 7 cut(s) 613, 658, 846, 1111, 2435, 2755, 3163
Eco57I CTGAAG 2 cut(s) 2543, 2979
Eco88I CYCGRG 1 cut(s) 194
EcoNI CCTNNNNNAGG 2 cut(s) 739, 1567
EcoO109I RGGNCCY 6 cut(s) 171, 613, 658, 1111, 2435, 3163
EcoRI GAATTC 1 cut(s) 1850
EcoRII CCWGG 8 cut(s) 101, 199, 675, 1390, 1548, 1838, 2259, 2431
EcoT14I CCWWGG 2 cut(s) 768, 3129
EcoT22I ATGCAT 1 cut(s) 1003
EcoT38I GRGCYC 2 cut(s) 175, 3124
ErhI CCWWGG 2 cut(s) 768, 3129
Esp3I CGTCTC 2 cut(s) 119, 685
FalI AAGNNNNNCTT 4 cut(s) 936, 968, 2699, 2731
FaqI GGGAC 2 cut(s) 1097, 1840
FauI CCCGC 3 cut(s) 80, 1582, 1617
FauNDI CATATG 1 cut(s) 1033
FriOI GRGCYC 2 cut(s) 175, 3124
FspBI CTAG 5 cut(s) 119, 1107, 1184, 1466, 2568
GlaI GCGC 2 cut(s) 319, 1662
GsaI CCCAGC 1 cut(s) 2601
GsuI CTGGAG 7 cut(s) 305, 480, 709, 2334, 2750, 2847, 3276
HaeII RGCGCY 1 cut(s) 1664
HapII CCGG 6 cut(s) 195, 1277, 1731, 2753, 3258, 3300
HgaI GACGC 2 cut(s) 740, 2756
HhaI GCGC 2 cut(s) 320, 1663
Hin1I GRCGYC 1 cut(s) 732
Hin6I GCGC 2 cut(s) 318, 1661
HinP1I GCGC 2 cut(s) 318, 1661
HincII GTYRAC 4 cut(s) 630, 1221, 1485, 2877
HindII GTYRAC 4 cut(s) 630, 1221, 1485, 2877
HindIII AAGCTT 3 cut(s) 383, 1577, 3068
HpaII CCGG 6 cut(s) 195, 1277, 1731, 2753, 3258, 3300
HphI GGTGA 4 cut(s) 500, 866, 1655, 3016
Hpy99I CGWCG 1 cut(s) 734
HpyCH4IV ACGT 4 cut(s) 694, 1081, 2647, 2685
HpySE526I ACGT 4 cut(s) 694, 1081, 2647, 2685
Hsp92I GRCGYC 1 cut(s) 732
HspAI GCGC 2 cut(s) 318, 1661
KflI GGGWCCC 1 cut(s) 1111
Kpn2I TCCGGA 2 cut(s) 1276, 2752
LguI GCTCTTC 2 cut(s) 86, 1215
LmnI GCTCC 4 cut(s) 30, 1572, 2755, 2820
Lsp1109I GCAGC 9 cut(s) 280, 308, 514, 943, 971, 1088, 1396, 2347, 2551
LweI GCATC 9 cut(s) 262, 539, 1842, 2145, 2180, 2275, 2299, 2320, 3232
MaeI CTAG 5 cut(s) 119, 1107, 1184, 1466, 2568
MaeII ACGT 4 cut(s) 694, 1081, 2647, 2685
MaeIII GTNAC 6 cut(s) 593, 683, 794, 1148, 1252, 2866
MfeI CAATTG 3 cut(s) 2265, 3108, 3114
MflI RGATCY 4 cut(s) 787, 1429, 2107, 2385
MhlI GDGCHC 4 cut(s) 175, 2202, 2922, 3124
MlyI GAGTC 3 cut(s) 1822, 1871, 2786
MmeI TCCRAC 3 cut(s) 612, 912, 2049
Mph1103I ATGCAT 1 cut(s) 1003
MroI TCCGGA 2 cut(s) 1276, 2752
MroXI GAANNNNTTC 3 cut(s) 861, 947, 1499
MslI CAYNNNNRTG 3 cut(s) 890, 2804, 2925
MspI CCGG 6 cut(s) 195, 1277, 1731, 2753, 3258, 3300
MunI CAATTG 3 cut(s) 2265, 3108, 3114
MvaI CCWGG 8 cut(s) 103, 201, 677, 1392, 1550, 1840, 2261, 2433
NciI CCSGG 3 cut(s) 195, 196, 3300
NcoI CCATGG 1 cut(s) 768
NdeI CATATG 1 cut(s) 1033
NheI GCTAGC 1 cut(s) 1465
NlaIV GGNNCC 7 cut(s) 173, 615, 906, 1112, 1113, 1939, 2437
NmuCI GTSAC 4 cut(s) 593, 683, 1252, 2866
NsiI ATGCAT 1 cut(s) 1003
NspI RCATGY 4 cut(s) 266, 361, 1933, 2524
PacI TTAATTAA 1 cut(s) 3189
PaeI GCATGC 1 cut(s) 361
PagI TCATGA 2 cut(s) 838, 3084
PaqCI CACCTGC 1 cut(s) 476
PasI CCCWGGG 1 cut(s) 1839
PceI AGGCCT 1 cut(s) 3128
PciI ACATGT 2 cut(s) 1929, 2520
PciSI GCTCTTC 2 cut(s) 86, 1215
PdmI GAANNNNTTC 3 cut(s) 861, 947, 1499
PfeI GAWTC 9 cut(s) 397, 1192, 1271, 1500, 1792, 1874, 2417, 2608, 3286
PflFI GACNNNGTC 1 cut(s) 2459
PflMI CCANNNNNTGG 2 cut(s) 1397, 2675
PfoI TCCNGGA 2 cut(s) 1390, 1548
PleI GAGTC 3 cut(s) 1822, 1870, 2786
PpsI GAGTC 3 cut(s) 1822, 1870, 2786
Ppu21I YACGTR 2 cut(s) 1082, 2686
PpuMI RGGWCCY 5 cut(s) 613, 658, 1111, 2435, 3163
PscI ACATGT 2 cut(s) 1929, 2520
PsiI TTATAA 1 cut(s) 621
Psp5II RGGWCCY 5 cut(s) 613, 658, 1111, 2435, 3163
Psp6I CCWGG 8 cut(s) 101, 199, 675, 1390, 1548, 1838, 2259, 2431
PspFI CCCAGC 1 cut(s) 2597
PspGI CCWGG 8 cut(s) 101, 199, 675, 1390, 1548, 1838, 2259, 2431
PspN4I GGNNCC 7 cut(s) 173, 615, 906, 1112, 1113, 1939, 2437
PspOMI GGGCCC 1 cut(s) 171
PspPPI RGGWCCY 5 cut(s) 613, 658, 1111, 2435, 3163
PstI CTGCAG 3 cut(s) 298, 961, 1414
PsuI RGATCY 4 cut(s) 787, 1429, 2107, 2385
PsyI GACNNNGTC 1 cut(s) 2459
RseI CAYNNNNRTG 3 cut(s) 890, 2804, 2925
SapI GCTCTTC 2 cut(s) 86, 1215
ScaI AGTACT 2 cut(s) 64, 1103
SchI GAGTC 3 cut(s) 1822, 1871, 2786
SduI GDGCHC 4 cut(s) 175, 2202, 2922, 3124
SfaNI GCATC 9 cut(s) 262, 539, 1842, 2145, 2180, 2275, 2299, 2320, 3232
SfcI CTRYAG 4 cut(s) 294, 708, 957, 1410
SinI GGWCC 7 cut(s) 613, 658, 846, 1111, 2435, 2755, 3163
SmaI CCCGGG 1 cut(s) 196
SmiMI CAYNNNNRTG 3 cut(s) 890, 2804, 2925
SmlI CTYRAG 3 cut(s) 662, 1433, 2537
SmoI CTYRAG 3 cut(s) 662, 1433, 2537
SnaBI TACGTA 1 cut(s) 2686
SphI GCATGC 1 cut(s) 361
SrfI GCCCGGGC 1 cut(s) 196
SseBI AGGCCT 1 cut(s) 3128
SsiI CCGC 8 cut(s) 74, 77, 87, 191, 543, 1589, 1624, 2371
SspI AATATT 3 cut(s) 2072, 2482, 3222
SspMI CTAG 5 cut(s) 119, 1107, 1184, 1466, 2568
StuI AGGCCT 1 cut(s) 3128
StyI CCWWGG 2 cut(s) 768, 3129
TaiI ACGT 4 cut(s) 697, 1084, 2650, 2688
TaqI TCGA 6 cut(s) 139, 278, 307, 521, 729, 3044
TatI WGTACW 3 cut(s) 62, 350, 1101
TauI GCSGC 4 cut(s) 77, 193, 546, 2374
TfiI GAWTC 9 cut(s) 397, 1192, 1271, 1500, 1792, 1874, 2417, 2608, 3286
TseFI GTSAC 4 cut(s) 593, 683, 1252, 2866
TseI GCWGC 9 cut(s) 293, 296, 527, 956, 959, 1076, 1409, 2360, 2564
Tsp45I GTSAC 4 cut(s) 593, 683, 1252, 2866
TspGWI ACGGA 1 cut(s) 782
TspMI CCCGGG 1 cut(s) 194
Tth111I GACNNNGTC 1 cut(s) 2459
Van91I CCANNNNNTGG 2 cut(s) 1397, 2675
VpaK11BI GGWCC 7 cut(s) 613, 658, 846, 1111, 2435, 2755, 3163
XagI CCTNNNNNAGG 2 cut(s) 739, 1567
XapI RAATTY 5 cut(s) 605, 1529, 1850, 2273, 2335
XceI RCATGY 4 cut(s) 266, 361, 1933, 2524
XmaI CCCGGG 1 cut(s) 194
XmnI GAANNNNTTC 3 cut(s) 861, 947, 1499
XspI CTAG 5 cut(s) 119, 1107, 1184, 1466, 2568
ZrmI AGTACT 2 cut(s) 64, 1103
Zsp2I ATGCAT 1 cut(s) 1003
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.