Rh4AG399500

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000598
Physical Location & Seq
Reverse (-)
70330278 .. 70334614
4337 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG399500.1

Sequence Viewer

Length: 2073 bp
ATGCCCATCAAAAGTATTGGCCTGTCTGCCATTGATATATCAGAACTCCAGAATCCATCGAACTGGTTGAGCTGCTTCCCTCGAGTTGCAGTGAAAGAGCTCAAGGCATTTGGCTTGGGCTGTGACTGGAGGCGCTCCTTTATTACCACAGACATGAACCCATACTTTGACAAGTTTGTGAGATGGCAGATGAGGAAACTAAAATCTATGGGTAAGATTGTGAAGGATGTCCGTTACACAATCTACTCTCCTTTGGATGGCCAGCCATGTGCAGATCATGATAGGGAAACTGGTGAAGGAGTTCGGCCCCAAGAGTACACCATCATCAAAATGGAGGTGGTGGCACCTTTTCCTCCTAAACTGGGAGTGTTGAAGGGAAAGAAAGTCTTCCTTGCTGCCGCAACATTGAGACCTGAGACTTCTTATGGTCAAACAAATGCATGGGTGTTGCCTGATGGCAAGTATGGAGCCTTTGAAATCAATGAAACAGATGTCTTCATTCTTACGCAGAGAGCAGCACTTAATCTTGCCTATCAGAAGTACTCTAGGGTTCCAGAAAAGCCTTCTTGCTTGGTTGAGATGACTGGCTATGATTTGATTGGCCTTCCATTGAAGTCTCCACTTGCACTCAATCAGATCATTTATGCCCTTCCTATGTTGACCGTCCTAACAGACAAAGGTACTGGGATATTGACTAGTGTACCTCGTGATGCTCCTGATGATTATATGGCCTTGGAAGATTTAAAATTGAAACCAGCTTTTAGAGAAAAATATGGTGTGAAGGATGAATGGGTCATGCCATTTGATATCATTCCAATAATCAAAATTCCGGAATATGGAGACAAGGCTGCTAAAAAGGTTGTGGAAGATCTCAAAATCAAAAGTCAGAATGAGATAGAGAAGCTAGCAGAAGCCAAGAGGTTGACATCCTTGAAAGGTTTAACTGAAGGAAAACTGCTTGTGGGAGAATTCAAAGAAAGGATAGTCCAGGAGGCAAAACCATTGATAAGGAGCAGGCTAATAGAGGCAGGTGATGCAATCATGTATAGCGAGCCTGAAAAGCAGGTAGTTTCACGATCTGGTGATGAATGTGTTGTTGCTCTTATAGATCAATGGTGCATCACATATGGGGAAGCAGAATGGAAAAAAACTGCTCAGGAGTGCTTGTCATGCATAAATTTATATTCTGACACGACACGACATGGATTTGTACACACATTGAATTGGTTGAATCAGTGGGCTTGTTCTCGATCTTTTGGTCTTGGTACACGCATTCCCTGGGATGAAGAATACTTGGTCGAGCCGTTATCCGATTCAACTCTTTACATGGCTTATTACACCGTTGCTCACCTGTTACACAATGGAGACATGTATGGCAGCTCCAACAGCACATCTGCAGTAAGAGCTGAACAAATGACTGATGAGGTTTGGGATTTTATTTTCTGTGATGGCTCGTATCCACAATCATCTGATATCACACCATCAATTCTTAACAAGATGAAGCAGGAGTTTGAATATTGGTATCCTTTTGATCTTGAGGTGTCTGGTAAGGATCTAATCCAGAATCATACCTTCTGCATTTACAACCATACTGCACTTCTGCGCAAGAAGCACTGGCCTAGTGCGTTTAGATGCAATGGGCACCTGATGCTCAAATCCACGAAGAATGCTAATGGTGCTGGAAATTTTATATCATTGAGACACGCAATTGATCACTATTCTGCTGATGCCACGCGGTTTGCTTTGGCTGATATTAGTGATGGTATTGACGATGCATACTTTTCATTTGAAACTGCAAATGCGGCCATCATACGTCTTACTAAGGAGATAGATTGGATTGATCGGATGAAAGATAAAGATTTGGGTAAAGACTCTTCTCTGAGAATAGGTTCCCCTTGTACCTTCTCTGACCGGGTCTTTCTGAATGAAATAAATATTTCTGTGAAGAGGACTGACCAAAATTACCAGGGTTGCATGTTCAGGGAAGCCCTAAAGACTGGCTTTTATGATCTTCAAGCTGCCAGGGATTGGTACAGGATTTCATGTGGTGGTACTAAACTACTAATCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

690

Amino Acids

78.4

Weight (kDa)

6.2

Isoelectric Point (pI)

39.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1 PF00133 30 - 592 5.1e-12 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g PF09334 501 - 600 5e-06 tRNA synthetases class I (M)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000598)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09620
fragaria_vesca FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_3g34581 FvH4_4g33720 FvH4_4g33720
malus_domestica MD08G1244600.v1.1 MD15G1439400.v1.1
prunus_persica Prupe.1G582000_v2.0.a1 Prupe.2G015100_v2.0.a1 Prupe.2G123100_v2.0.a1
pyrus_communis pycom08g21210 pycom08g21220 pycom15g38730
rosa_chinensis RchiOBHm_Chr1g0336121 RchiOBHm_Chr1g0353811 RchiOBHm_Chr1g0373331 RchiOBHm_Chr4g0443191 RchiOBHm_Chr4g0443201 RchiOBHm_Chr4g0443211
rosa_laevigata RLG00000003122 RLG00000005948 RLG00000026826 RLG00000028251 RLG00000028252 RLG00000028253 RLG00000029377
rosa_multiflora Rmu_co8002498.1_g000001 Rmu_co8262279.1_g000001 Rmu_co8314139.1_g000001 Rmu_co8393711.1_g000001 Rmu_co8419167.1_g000001 Rmu_sc0007968.1_g000004 Rmu_sc0018919.1_g000004 Rmu_sc0027447.1_g000001
rosa_roxburghii Rroxscaffold_4G00284260 Rroxscaffold_4G00301490 Rroxscaffold_4G00315860 Rroxscaffold_5G00383790 Rroxscaffold_5G00383800
rosa_rugosa Rorug01G0123100 Rorug01G0238700 Rorug01G0377100 Rorug01G0377100 Rorug01G0377200 Rorug01G0377300 Rorug04G0344700 Rorug05G0347900.1
rosa_samantha Rh1AG143800 Rh1AG250300 Rh1AG386300 Rh1BG113400 Rh1BG220800 Rh1BG220900 Rh1BG350400 Rh1CG135500 Rh1CG233100 Rh1CG363500 Rh1DG150200 Rh1DG247700 Rh1DG247800 Rh1DG381300 Rh4AG399400 Rh4AG399500 Rh4BG411000 Rh4BG411100 Rh4BG411200 Rh4CG426500 Rh4CG426600 Rh4DG406100 Rh4DG406200 Rh4DG406300
rosa_wichuraiana Rw0G015190 Rw1G021850 Rw1G034870 Rw4G034440 Rw4G034450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1019
Acc16I TGCGCA 1 cut(s) 1604
Acc36I ACCTGC 2 cut(s) 1019, 1054
AccB1I GGYRCC 2 cut(s) 343, 1641
AccB7I CCANNNNNTGG 1 cut(s) 2028
AccII CGCG 1 cut(s) 1735
AccIII TCCGGA 1 cut(s) 829
AciI CCGC 3 cut(s) 399, 1735, 1802
AclWI GGATC 1 cut(s) 1560
AcoI YGGCCR 2 cut(s) 259, 1803
AcsI RAATTY 4 cut(s) 825, 968, 1177, 1684
AcuI CTGAAG 1 cut(s) 966
AfaI GTAC 9 cut(s) 317, 542, 682, 702, 1212, 1267, 1900, 2033, 2053
AfiI CCNNNNNNNGG 4 cut(s) 257, 362, 836, 2028
AflIII ACRYGT 1 cut(s) 1368
AhlI ACTAGT 1 cut(s) 695
AjnI CCWGG 4 cut(s) 987, 1277, 1965, 2021
AluBI AGCT 7 cut(s) 72, 100, 758, 904, 1380, 1406, 2018
AluI AGCT 7 cut(s) 72, 100, 758, 904, 1380, 1406, 2018
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 6 cut(s) 403, 410, 621, 834, 1359, 1693
AlwI GGATC 1 cut(s) 1560
Ama87I CYCGRG 1 cut(s) 81
Aor13HI TCCGGA 1 cut(s) 829
AoxI GGCC 7 cut(s) 19, 259, 305, 601, 729, 1616, 1803
ApeKI GCWGC 6 cut(s) 72, 395, 515, 848, 1377, 2018
ApoI RAATTY 4 cut(s) 825, 968, 1177, 1684
Asp700I GAANNNNTTC 3 cut(s) 300, 386, 1888
AspLEI GCGC 2 cut(s) 135, 1605
AspS9I GGNCC 1 cut(s) 306
AsuC2I CCSGG 1 cut(s) 1913
AsuHPI GGTGA 4 cut(s) 305, 1043, 1094, 1340
AsuNHI GCTAGC 1 cut(s) 904
AvaI CYCGRG 1 cut(s) 81
BaeGI GKGCMC 1 cut(s) 1644
BaeI ACNNNNGTAYC 2 cut(s) 684, 717
BalI TGGCCA 1 cut(s) 261
BanI GGYRCC 2 cut(s) 343, 1641
BanII GRGCYC 1 cut(s) 102
BarI GAAGNNNNNNTAC 2 cut(s) 1858, 1890
BauI CACGAG 1 cut(s) 705
BbsI GAAGAC 2 cut(s) 379, 487
Bbv12I GWGCWC 1 cut(s) 102
BbvI GCAGC 6 cut(s) 59, 382, 527, 835, 1389, 2005
BceAI ACGGC 1 cut(s) 1288
BciT130I CCWGG 4 cut(s) 989, 1279, 1967, 2023
BciVI GTATCC 2 cut(s) 1467, 1533
BclI TGATCA 1 cut(s) 1711
BcnI CCSGG 1 cut(s) 1913
BcoDI GTCTC 6 cut(s) 403, 410, 621, 834, 1359, 1693
BcuI ACTAGT 1 cut(s) 695
BfaI CTAG 4 cut(s) 546, 696, 905, 1620
BfmI CTRYAG 1 cut(s) 1395
BfoI RGCGCY 1 cut(s) 136
BfuAI ACCTGC 2 cut(s) 1019, 1054
BfuI GTATCC 2 cut(s) 1467, 1533
BglII AGATCT 1 cut(s) 868
BisI GCNGC 8 cut(s) 73, 396, 399, 516, 849, 1378, 1803, 2019
BlsI GCNGC 8 cut(s) 74, 397, 400, 517, 850, 1379, 1804, 2020
BmcAI AGTACT 1 cut(s) 542
Bme1390I CCNGG 5 cut(s) 989, 1279, 1913, 1967, 2023
BmeT110I CYCGRG 1 cut(s) 81
BmgT120I GGNCC 1 cut(s) 306
BmiI GGNNCC 6 cut(s) 308, 345, 469, 552, 1643, 1891
BmrFI CCNGG 5 cut(s) 989, 1279, 1913, 1967, 2023
BmrI ACTGGG 2 cut(s) 371, 693
BmsI GCATC 7 cut(s) 700, 1024, 1128, 1622, 1638, 1717, 1762
BmtI GCTAGC 1 cut(s) 908
BmuI ACTGGG 2 cut(s) 371, 693
BpiI GAAGAC 2 cut(s) 379, 487
BpmI CTGGAG 2 cut(s) 32, 148
Bpu10I CCTNAGC 1 cut(s) 1155
BpuEI CTTGAG 2 cut(s) 86, 1556
BpuMI CCSGG 1 cut(s) 1913
BsaI GGTCTC 1 cut(s) 403
BsaJI CCNNGG 5 cut(s) 732, 1277, 1278, 1966, 2022
BsaWI WCCGGW 1 cut(s) 829
BsaXI ACNNNNNCTCC 2 cut(s) 1151, 1181
Bsc4I CCNNNNNNNGG 4 cut(s) 257, 362, 836, 2028
Bse1I ACTGG 8 cut(s) 68, 131, 295, 366, 589, 688, 1619, 2002
Bse3DI GCAATG 1 cut(s) 1642
BseAI TCCGGA 1 cut(s) 829
BseBI CCWGG 4 cut(s) 989, 1279, 1967, 2023
BseDI CCNNGG 5 cut(s) 732, 1277, 1278, 1966, 2022
BseGI GGATG 6 cut(s) 232, 262, 790, 926, 1288, 1851
BseLI CCNNNNNNNGG 4 cut(s) 257, 362, 836, 2028
BseMI GCAATG 1 cut(s) 1642
BseMII CTCAG 3 cut(s) 405, 1169, 1871
BseNI ACTGG 8 cut(s) 68, 131, 295, 366, 589, 688, 1619, 2002
BseSI GKGCMC 1 cut(s) 1644
BseXI GCAGC 6 cut(s) 59, 382, 527, 835, 1389, 2005
BsgI GTGCAG 2 cut(s) 291, 1578
Bsh1236I CGCG 1 cut(s) 1735
BshFI GGCC 7 cut(s) 21, 261, 307, 603, 731, 1618, 1805
BshNI GGYRCC 2 cut(s) 343, 1641
BsiHKAI GWGCWC 1 cut(s) 102
BsiHKCI CYCGRG 1 cut(s) 81
BsiSI CCGG 2 cut(s) 830, 1912
BslI CCNNNNNNNGG 4 cut(s) 257, 362, 836, 2028
BsmAI GTCTC 6 cut(s) 403, 410, 621, 834, 1359, 1693
BsmI GAATGC 2 cut(s) 1272, 1672
BsnI GGCC 7 cut(s) 21, 261, 307, 603, 731, 1618, 1805
Bso31I GGTCTC 1 cut(s) 403
BsoBI CYCGRG 1 cut(s) 81
Bsp1286I GDGCHC 2 cut(s) 102, 1644
Bsp13I TCCGGA 1 cut(s) 829
Bsp1407I TGTACA 1 cut(s) 1210
BspACI CCGC 3 cut(s) 399, 1735, 1802
BspANI GGCC 7 cut(s) 21, 261, 307, 603, 731, 1618, 1805
BspCNI CTCAG 3 cut(s) 406, 1168, 1872
BspEI TCCGGA 1 cut(s) 829
BspFNI CGCG 1 cut(s) 1735
BspHI TCATGA 1 cut(s) 277
BspLI GGNNCC 6 cut(s) 308, 345, 469, 552, 1643, 1891
BspMAI CTGCAG 1 cut(s) 1399
BspMI ACCTGC 2 cut(s) 1019, 1054
BspOI GCTAGC 1 cut(s) 908
BspPI GGATC 1 cut(s) 1560
BspT107I GGYRCC 2 cut(s) 343, 1641
BspTNI GGTCTC 1 cut(s) 403
BsrDI GCAATG 1 cut(s) 1642
BsrGI TGTACA 1 cut(s) 1210
BsrI ACTGG 8 cut(s) 68, 131, 295, 366, 589, 688, 1619, 2002
BssECI CCNNGG 5 cut(s) 732, 1277, 1278, 1966, 2022
BssSI CACGAG 1 cut(s) 705
BssT1I CCWWGG 1 cut(s) 732
Bst2BI CACGAG 1 cut(s) 705
Bst2UI CCWGG 4 cut(s) 989, 1279, 1967, 2023
Bst4CI ACNGT 2 cut(s) 664, 1342
Bst6I CTCTTC 2 cut(s) 1879, 1940
BstAPI GCANNNNNTGC 2 cut(s) 1034, 1648
BstAUI TGTACA 1 cut(s) 1210
BstC8I GCNNGC 4 cut(s) 263, 906, 1016, 1052
BstDEI CTNAG 4 cut(s) 414, 1155, 1821, 1880
BstF5I GGATG 6 cut(s) 232, 262, 790, 926, 1288, 1851
BstFNI CGCG 1 cut(s) 1735
BstH2I RGCGCY 1 cut(s) 136
BstHHI GCGC 2 cut(s) 135, 1605
BstMAI GTCTC 6 cut(s) 403, 410, 621, 834, 1359, 1693
BstMWI GCNNNNNNNGC 9 cut(s) 1034, 1060, 1170, 1386, 1403, 1609, 1648, 1676, 1802
BstNI CCWGG 4 cut(s) 989, 1279, 1967, 2023
BstNSI RCATGY 2 cut(s) 1372, 1978
BstSCI CCNGG 5 cut(s) 987, 1277, 1911, 1965, 2021
BstSFI CTRYAG 1 cut(s) 1395
BstSLI GKGCMC 1 cut(s) 1644
BstUI CGCG 1 cut(s) 1735
BstV1I GCAGC 6 cut(s) 59, 382, 527, 835, 1389, 2005
BstV2I GAAGAC 2 cut(s) 379, 487
BstX2I RGATCY 2 cut(s) 868, 1552
BstXI CCANNNNNNTGG 1 cut(s) 63
BstYI RGATCY 2 cut(s) 868, 1552
BsuI GTATCC 2 cut(s) 1467, 1533
BsuRI GGCC 7 cut(s) 21, 261, 307, 603, 731, 1618, 1805
BtsCI GGATG 6 cut(s) 232, 262, 790, 926, 1288, 1851
BtsI GCAGTG 1 cut(s) 96
BtsIMutI CAGTG 3 cut(s) 96, 1241, 1612
BveI ACCTGC 2 cut(s) 1019, 1054
Cac8I GCNNGC 4 cut(s) 263, 906, 1016, 1052
CciI TCATGA 1 cut(s) 277
CfoI GCGC 2 cut(s) 135, 1605
Cfr13I GGNCC 1 cut(s) 306
Csp6I GTAC 9 cut(s) 316, 541, 681, 701, 1211, 1266, 1899, 2032, 2052
CspCI CAANNNNNGTGG 2 cut(s) 1720, 1755
CviQI GTAC 9 cut(s) 316, 541, 681, 701, 1211, 1266, 1899, 2032, 2052
DdeI CTNAG 4 cut(s) 414, 1155, 1821, 1880
DraI TTTAAA 1 cut(s) 744
EaeI YGGCCR 2 cut(s) 259, 1803
Eam1104I CTCTTC 2 cut(s) 1879, 1940
EarI CTCTTC 2 cut(s) 1879, 1940
Ecl136II GAGCTC 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 732
Eco24I GRGCYC 1 cut(s) 102
Eco31I GGTCTC 1 cut(s) 403
Eco32I GATATC 2 cut(s) 808, 1474
Eco53kI GAGCTC 1 cut(s) 100
Eco57I CTGAAG 1 cut(s) 966
Eco88I CYCGRG 1 cut(s) 81
EcoICRI GAGCTC 1 cut(s) 100
EcoRI GAATTC 1 cut(s) 968
EcoRII CCWGG 4 cut(s) 987, 1277, 1965, 2021
EcoRV GATATC 2 cut(s) 808, 1474
EcoT14I CCWWGG 1 cut(s) 732
EcoT22I ATGCAT 3 cut(s) 442, 1175, 1777
EcoT38I GRGCYC 1 cut(s) 102
ErhI CCWWGG 1 cut(s) 732
FalI AAGNNNNNCTT 6 cut(s) 371, 403, 375, 407, 1985, 2017
FauNDI CATATG 1 cut(s) 1126
FbaI TGATCA 1 cut(s) 1711
Fnu4HI GCNGC 8 cut(s) 73, 396, 399, 516, 849, 1378, 1803, 2019
FokI GGATG 6 cut(s) 239, 269, 797, 913, 1295, 1858
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 8 cut(s) 73, 396, 399, 516, 849, 1378, 1803, 2019
FspBI CTAG 4 cut(s) 546, 696, 905, 1620
FspI TGCGCA 1 cut(s) 1604
GlaI GCGC 2 cut(s) 134, 1604
GluI GCNGC 8 cut(s) 73, 396, 399, 516, 849, 1378, 1803, 2019
GsuI CTGGAG 2 cut(s) 32, 148
HaeII RGCGCY 1 cut(s) 136
HaeIII GGCC 7 cut(s) 21, 261, 307, 603, 731, 1618, 1805
HapII CCGG 2 cut(s) 830, 1912
HhaI GCGC 2 cut(s) 135, 1605
Hin6I GCGC 2 cut(s) 133, 1603
HinP1I GCGC 2 cut(s) 133, 1603
HincII GTYRAC 2 cut(s) 660, 924
HindII GTYRAC 2 cut(s) 660, 924
HinfI GANTC 5 cut(s) 52, 1231, 1313, 1564, 1871
HpaII CCGG 2 cut(s) 830, 1912
HphI GGTGA 4 cut(s) 305, 1043, 1094, 1340
Hpy166II GTNNAC 6 cut(s) 318, 660, 701, 924, 1213, 1268
Hpy8I GTNNAC 6 cut(s) 318, 660, 701, 924, 1213, 1268
HpyCH4III ACNGT 2 cut(s) 664, 1342
HpyCH4IV ACGT 1 cut(s) 1813
HpyF10VI GCNNNNNNNGC 9 cut(s) 1034, 1060, 1170, 1386, 1403, 1609, 1648, 1676, 1802
HpyF3I CTNAG 4 cut(s) 414, 1155, 1821, 1880
HpySE526I ACGT 1 cut(s) 1813
HspAI GCGC 2 cut(s) 133, 1603
Kpn2I TCCGGA 1 cut(s) 829
Ksp22I TGATCA 1 cut(s) 1711
LmnI GCTCC 5 cut(s) 140, 467, 718, 1011, 1385
Lsp1109I GCAGC 6 cut(s) 59, 382, 527, 835, 1389, 2005
LweI GCATC 7 cut(s) 700, 1024, 1128, 1622, 1638, 1717, 1762
MaeI CTAG 4 cut(s) 546, 696, 905, 1620
MaeII ACGT 1 cut(s) 1813
MaeIII GTNAC 3 cut(s) 122, 233, 1353
MboII GAAGA 9 cut(s) 379, 487, 749, 878, 1298, 1675, 1866, 1957, 2003
MfeI CAATTG 1 cut(s) 1707
MflI RGATCY 2 cut(s) 868, 1552
MhlI GDGCHC 2 cut(s) 102, 1644
MlsI TGGCCA 1 cut(s) 261
MluCI AATT 9 cut(s) 746, 825, 968, 1177, 1222, 1485, 1684, 1707, 1960
MluNI TGGCCA 1 cut(s) 261
MlyI GAGTC 1 cut(s) 1865
MmeI TCCRAC 1 cut(s) 1407
Mox20I TGGCCA 1 cut(s) 261
Mph1103I ATGCAT 3 cut(s) 442, 1175, 1777
MroI TCCGGA 1 cut(s) 829
MroXI GAANNNNTTC 3 cut(s) 300, 386, 1888
MscI TGGCCA 1 cut(s) 261
MseI TTAA 4 cut(s) 522, 743, 941, 1491
MslI CAYNNNNRTG 2 cut(s) 152, 329
Msp20I TGGCCA 1 cut(s) 261
MspI CCGG 2 cut(s) 830, 1912
MspR9I CCNGG 5 cut(s) 989, 1279, 1913, 1967, 2023
MunI CAATTG 1 cut(s) 1707
Mva1269I GAATGC 2 cut(s) 1272, 1672
MvaI CCWGG 4 cut(s) 989, 1279, 1967, 2023
MvnI CGCG 1 cut(s) 1735
MwoI GCNNNNNNNGC 9 cut(s) 1034, 1060, 1170, 1386, 1403, 1609, 1648, 1676, 1802
NciI CCSGG 1 cut(s) 1913
NdeI CATATG 1 cut(s) 1126
NheI GCTAGC 1 cut(s) 904
NlaIV GGNNCC 6 cut(s) 308, 345, 469, 552, 1643, 1891
NmuCI GTSAC 1 cut(s) 122
NsbI TGCGCA 1 cut(s) 1604
NsiI ATGCAT 3 cut(s) 442, 1175, 1777
NspI RCATGY 2 cut(s) 1372, 1978
PaeR7I CTCGAG 1 cut(s) 81
PagI TCATGA 1 cut(s) 277
PaqCI CACCTGC 1 cut(s) 1019
PasI CCCWGGG 1 cut(s) 1278
PciI ACATGT 1 cut(s) 1368
PctI GAATGC 2 cut(s) 1272, 1672
PdmI GAANNNNTTC 3 cut(s) 300, 386, 1888
PfeI GAWTC 4 cut(s) 52, 1231, 1313, 1564
PflFI GACNNNGTC 1 cut(s) 1913
PflMI CCANNNNNTGG 1 cut(s) 2028
PfoI TCCNGGA 1 cut(s) 987
PkrI GCNGC 8 cut(s) 74, 397, 400, 517, 850, 1379, 1804, 2020
PleI GAGTC 1 cut(s) 1865
PpsI GAGTC 1 cut(s) 1865
PscI ACATGT 1 cut(s) 1368
Psp124BI GAGCTC 1 cut(s) 102
Psp6I CCWGG 4 cut(s) 987, 1277, 1965, 2021
PspGI CCWGG 4 cut(s) 987, 1277, 1965, 2021
PspN4I GGNNCC 6 cut(s) 308, 345, 469, 552, 1643, 1891
PspPI GGNCC 1 cut(s) 306
PspXI VCTCGAGB 1 cut(s) 81
PstI CTGCAG 1 cut(s) 1399
PsuI RGATCY 2 cut(s) 868, 1552
PsyI GACNNNGTC 1 cut(s) 1913
RsaI GTAC 9 cut(s) 317, 542, 682, 702, 1212, 1267, 1900, 2033, 2053
RsaNI GTAC 9 cut(s) 316, 541, 681, 701, 1211, 1266, 1899, 2032, 2052
RseI CAYNNNNRTG 2 cut(s) 152, 329
SacI GAGCTC 1 cut(s) 102
SaqAI TTAA 4 cut(s) 522, 743, 941, 1491
SatI GCNGC 8 cut(s) 73, 396, 399, 516, 849, 1378, 1803, 2019
Sau96I GGNCC 1 cut(s) 306
ScaI AGTACT 1 cut(s) 542
SchI GAGTC 1 cut(s) 1865
ScrFI CCNGG 5 cut(s) 989, 1279, 1913, 1967, 2023
SduI GDGCHC 2 cut(s) 102, 1644
SfaNI GCATC 7 cut(s) 700, 1024, 1128, 1622, 1638, 1717, 1762
SfcI CTRYAG 1 cut(s) 1395
Sfr274I CTCGAG 1 cut(s) 81
SlaI CTCGAG 1 cut(s) 81
SmiMI CAYNNNNRTG 2 cut(s) 152, 329
SmlI CTYRAG 3 cut(s) 81, 101, 1535
SmoI CTYRAG 3 cut(s) 81, 101, 1535
SpeI ACTAGT 1 cut(s) 695
Sse9I AATT 9 cut(s) 746, 825, 968, 1177, 1222, 1485, 1684, 1707, 1960
SsiI CCGC 3 cut(s) 399, 1735, 1802
SspI AATATT 2 cut(s) 1517, 1936
SspMI CTAG 4 cut(s) 546, 696, 905, 1620
SstI GAGCTC 1 cut(s) 102
StyD4I CCNGG 5 cut(s) 987, 1277, 1911, 1965, 2021
StyI CCWWGG 1 cut(s) 732
TaaI ACNGT 2 cut(s) 664, 1342
TaiI ACGT 1 cut(s) 1816
TaqI TCGA 4 cut(s) 59, 82, 1249, 1299
TasI AATT 9 cut(s) 746, 825, 968, 1177, 1222, 1485, 1684, 1707, 1960
TatI WGTACW 3 cut(s) 315, 540, 1210
TauI GCSGC 2 cut(s) 401, 1805
TfiI GAWTC 4 cut(s) 52, 1231, 1313, 1564
Tru1I TTAA 4 cut(s) 522, 743, 941, 1491
Tru9I TTAA 4 cut(s) 522, 743, 941, 1491
TscAI CASTG 3 cut(s) 96, 1241, 1619
TseFI GTSAC 1 cut(s) 122
TseI GCWGC 6 cut(s) 72, 395, 515, 848, 1377, 2018
Tsp45I GTSAC 1 cut(s) 122
TspGWI ACGGA 1 cut(s) 221
TspRI CASTG 3 cut(s) 96, 1241, 1619
Tth111I GACNNNGTC 1 cut(s) 1913
Van91I CCANNNNNTGG 1 cut(s) 2028
XapI RAATTY 4 cut(s) 825, 968, 1177, 1684
XceI RCATGY 2 cut(s) 1372, 1978
XcmI CCANNNNNNNNNTGG 1 cut(s) 328
XhoI CTCGAG 1 cut(s) 81
XmnI GAANNNNTTC 3 cut(s) 300, 386, 1888
XspI CTAG 4 cut(s) 546, 696, 905, 1620
ZrmI AGTACT 1 cut(s) 542
Zsp2I ATGCAT 3 cut(s) 442, 1175, 1777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.