Rh1CG233100

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0000598
Physical Location & Seq
Reverse (-)
49011916 .. 49022485
10570 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG233100.1

Sequence Viewer

Length: 3120 bp
ATGAAGTCAATAAGGAGAGACCGTCTTCGAAAGATTGAGGAAGACGCTCAAAGACGGTGGGAAGAGAAGCAAGTTTTCAGGGCTGAACCCCGTGAAAATCCTCCCCAACCAGGAGAGAAGTTTTTCGGAAATTTCCCTTTCCCGTACATGAATGGTTTCCTTCATCTGGGACATGCATTCACCATCTCCAAGCTTGAGTTTGCTGCCGCTTATCATAGGCTAAGAGGTGTCAATGTGCTCTTACCATTTGCTTTCCATTGCACCGGAATGCCCATCAAAGCTGCCGCCGATAAACTTGCAAGAGAGCTCCAACAGTTTGGCTACCCTCCAGTATTTCCCATTGATGTGAAGCAAGATGAAATTGCAGCCAAAGCAAAAGCCGAAGCTGGAGCAGAGAAAAAACATAAGGGAAAGAAGGCCAAGGCTGCAGCAAAGTCCGCCGGACAAGTCTTCCAATGGGAAATTATGCGTGATGGTTTTGGATTGTCTGACAGTGAGATATCCGAGTTCCAGGACCCGGAAAAGTGGTTGAAGTTTTTCCCACCACGTGGGGTGGAAGACCTGAAAGCTTTTGGGTTGGGATGTGATTGGAGACGTTCCTTTATTACTACTGATATGAACCCTTTCTTTGACAACTTTGTGAAATGGCAGGTTAGGAAGCTCAAATCCAAGGGTAAGATTGTGAAAGATGTCCGGTACACTATATTCTCCCCATTCGATGGGCAGCCGTGTGCAGATCATGATAGGGCAAGAGGAGAAGGAGTTCAGCCACAAGAATACATTGTCATCAAAATGGAGGTAGTAGCACCTTTACCCTCAAAATTTGGATCAGTGTTGAAAGGGAGGAAGGTATTCCTAGCAGCAGCAACGTTGAGACCCGAGACCATGTATGGACAAACAAATGCATGGGTATTACCCGATGCTAAGTATGGAGCCTTTGAGATCAACGATACGGATGTCTTTATTCTTACACATAGAGCAGCACTTAATCTTGCTTATCAGAAGTACTCTAGGGTCCCAGAGAAACCTACTTGCTTGGTCGAACTGACTGGTTATGATTTGATTGGCCTTCCATTGAAGTCACCACTTGCATTCAGTCATATCATTTATGCCCTTCCTATGTTGACCATCCCAACTGACAAAGGCACTGGGATAGTGACCAGCGTACCTAGTGATGCTCCTGATGATTTTATGGCTCTGCATCATTTAAAGTCAAAATCTGCTTTGAGGGAAAAATATGGTGTGAAGGAAGAATGGGTGATGCCGTTTGAGGTTATCCCAATCATCAAAGAAGGAACACTGACCGTGGGAGAATTCGTAGGAAGGGAAGTGCAGGAGGCGAAACCCTTAATAAAAAGGAAGCTGATAGAGGCGGGAGAAGGAATTATATATTATGAACCTGAGGAGCAAGTTGTGTCCCGGTCGGGTGATGAATGTGTTGTGGCACTTACAGATCAGTGGTACATCACATATGGGGAAACTGAATGGAAAAAGATGGCTGAAGAGTGCTTGTCCAGCATGAGCTTATATTCCGATGAGACTCGGCATGGATTTGAGCACACTATAGGGTGGTTGAATCAGTGGGCTTGTTCACGATCTTTTGGACTTGGGACTCGTATCCCCTGGGATGAAGAATTCTTAGTTGAGTCACTGTCTGATTCAACTATTTACATGGCTTATTACACCATTGCTCATTTCTTACACGATGGAGATATTTATGGTGCCAGCAGGTCAGAAGTTAAACCTGAGCAAATGACTGATGACGTCTGGGATTTTATTTTCTGTGATGGCCCATATCCCAAGTCTTCTGACATCTCATCATCAATTCTTAAGAAAATGAAGAAAGAGTTTGAATATTGGTATCCTTTTGATCTACGAGTGTCAGGCAAGGATTTGATCCAAAACCATCTAGCCTTCTGCATATACAACCACACGGCTATTATGACGTCCAACAAGTACTGGCCTCGTGGGTTCAGATGTAATGGGCACCTCTTGCTAAATGGTGAGAAGATGTCCAAGTCTACAGGGAACTTTAGGACCCTTCGCCAGGCAATTGAGGAATTTTCTGCTGATGCCACTCGGTTCTCTTTGGCTGATGCTGGAGATGGTGTGGATGATGCAAATTTTGTATTTGTGACTGCCAATGCTGCAATCTTGCGGCTCACTAAAGAGATTGCATGGATTGAACAAGTTTTGGCTTCAGACTCTTGTTTGAGAACAGGTCCCCCATCTACTTATGCTGACAGGGTCTTTGCTAATGAGATAAACATTGCTGTACACAGGACTGAGCTGAATTACCGAGATTACATGTTCCGAGAAGCCCTCAAGACTGGGTTTTATGACCTTCAAGCAGCCAGGGATGAGTACAAGTTTTCGTGCGGTGCTGGGGGCATGAACCGTGATTTGGTATGGCGTTTTGTGGATGTGCAGACGCGTCTTATTACTCCAATCTGCCCACACTATGCAGAATATGTGTGGAGGGAACTTTTGAACAAGGAAGGGTTTGTGGTAAATGCAGGATGGCCTGTTGCTGATGCTTCAGACTTAACCCTCCAGAGTGCCAATAAGTATTTGCAAGACTTCATTGTTTTAATGAGGAAGCTTTTTCACAAGCAAGTTTCAGGTTCAAATAAAGGCCATAAGGGTACTCCAGTTACATTGATCACTGGAGAGAACAAGCTAACTGGCTTGGTATATGTTAATGAGCATTTTGATGGGTGGAAGGCAGAGTGCTTGAAAATACTTCAAATTCACTTTAACAGTGACACTCGTACATTTTCTTCTGACAAGCTGATACAGGAGGCGCTACAAAAAAGTTCTATTGGTCACTCTAAAGATTTTCGAGAAACCCTGAAGTTGTGTATGCCTTTCATAAGGTCAAAGATGGATGAAGCAATTGCGATTGGGGCTCGTGCCTTGGACTTAATGTTGCCATTTGGAGAGATTGAGGTCCTTCATGAGAACTTGGACTTGATCAAGAGGCAAATCGGTCTTCAAGAGTTGCATGTTTTGTCCTTTGCCAACCCTGATGCTCGGGCTTTAGCTGGTCCTCATGTCAAGCAGATAGATCGGAATCCTCCGTTTCCTGGGAGCCCCACTGCCATCTTCTTGACTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

1039

Amino Acids

118.11

Weight (kDa)

6.65

Isoelectric Point (pI)

45.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1 PF00133 17 - 98 5.6e-08 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1 PF00133 186 - 708 4.4e-17 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g PF09334 613 - 712 9.1e-09 tRNA synthetases class I (M)
tRNA-synt_1e PF01406 621 - 698 5.8e-07 tRNA synthetases class I (C) catalytic domain
Anticodon_1 PF08264 748 - 868 6e-13 Anticodon-binding domain of tRNA ligase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000598)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09620
fragaria_vesca FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_3g34581 FvH4_4g33720 FvH4_4g33720
malus_domestica MD08G1244600.v1.1 MD15G1439400.v1.1
prunus_persica Prupe.1G582000_v2.0.a1 Prupe.2G015100_v2.0.a1 Prupe.2G123100_v2.0.a1
pyrus_communis pycom08g21210 pycom08g21220 pycom15g38730
rosa_chinensis RchiOBHm_Chr1g0336121 RchiOBHm_Chr1g0353811 RchiOBHm_Chr1g0373331 RchiOBHm_Chr4g0443191 RchiOBHm_Chr4g0443201 RchiOBHm_Chr4g0443211
rosa_laevigata RLG00000003122 RLG00000005948 RLG00000026826 RLG00000028251 RLG00000028252 RLG00000028253 RLG00000029377
rosa_multiflora Rmu_co8002498.1_g000001 Rmu_co8262279.1_g000001 Rmu_co8314139.1_g000001 Rmu_co8393711.1_g000001 Rmu_co8419167.1_g000001 Rmu_sc0007968.1_g000004 Rmu_sc0018919.1_g000004 Rmu_sc0027447.1_g000001
rosa_roxburghii Rroxscaffold_4G00284260 Rroxscaffold_4G00301490 Rroxscaffold_4G00315860 Rroxscaffold_5G00383790 Rroxscaffold_5G00383800
rosa_rugosa Rorug01G0123100 Rorug01G0238700 Rorug01G0377100 Rorug01G0377100 Rorug01G0377200 Rorug01G0377300 Rorug04G0344700 Rorug05G0347900.1
rosa_samantha Rh1AG143800 Rh1AG250300 Rh1AG386300 Rh1BG113400 Rh1BG220800 Rh1BG220900 Rh1BG350400 Rh1CG135500 Rh1CG233100 Rh1CG363500 Rh1DG150200 Rh1DG247700 Rh1DG247800 Rh1DG381300 Rh4AG399400 Rh4AG399500 Rh4BG411000 Rh4BG411100 Rh4BG411200 Rh4CG426500 Rh4CG426600 Rh4DG406100 Rh4DG406200 Rh4DG406300
rosa_wichuraiana Rw0G015190 Rw1G021850 Rw1G034870 Rw4G034440 Rw4G034450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 1767, 1949
Acc36I ACCTGC 2 cut(s) 640, 1719
AccB1I GGYRCC 2 cut(s) 1721, 1986
AccB7I CCANNNNNTGG 2 cut(s) 548, 719
AccI GTMKAC 1 cut(s) 2021
AccII CGCG 1 cut(s) 2434
AciI CCGC 6 cut(s) 207, 285, 438, 1373, 2158, 2379
AclI AACGTT 1 cut(s) 869
AclWI GGATC 2 cut(s) 835, 1891
AcsI RAATTY 7 cut(s) 130, 821, 1313, 1634, 2060, 2122, 2748
AcuI CTGAAG 4 cut(s) 1521, 2184, 2523, 2873
AcvI CACGTG 1 cut(s) 548
AcyI GRCGYC 2 cut(s) 1764, 1946
AdeI CACNNNGTG 1 cut(s) 548
AfiI CCNNNNNNNGG 8 cut(s) 110, 166, 517, 548, 719, 2047, 2404, 3086
AflII CTTAAG 1 cut(s) 1829
AflIII ACRYGT 2 cut(s) 2307, 2432
AjnI CCWGG 6 cut(s) 109, 510, 1622, 2046, 2354, 3085
AjuI GAANNNNNNNTTGG 2 cut(s) 2387, 2419
AloI GAACNNNNNNTCC 2 cut(s) 747, 779
Alw21I GWGCWC 3 cut(s) 240, 309, 1560
Alw26I GTCTC 5 cut(s) 12, 586, 868, 875, 1532
AlwI GGATC 2 cut(s) 835, 1891
Ama87I CYCGRG 2 cut(s) 878, 3033
AoxI GGCC 6 cut(s) 417, 1066, 1789, 1961, 2522, 2635
ApoI RAATTY 7 cut(s) 130, 821, 1313, 1634, 2060, 2122, 2748
ArsI GACNNNNNNTTYG 2 cut(s) 2234, 2266
Asp700I GAANNNNTTC 6 cut(s) 122, 155, 536, 623, 762, 851
AspLEI GCGC 1 cut(s) 2806
AspS9I GGNCC 7 cut(s) 514, 1015, 1790, 2037, 2222, 2950, 3047
AsuC2I CCSGG 2 cut(s) 518, 1420
AsuHPI GGTGA 5 cut(s) 172, 1074, 1270, 1439, 2015
AsuII TTCGAA 1 cut(s) 28
AvaI CYCGRG 2 cut(s) 878, 3033
AvaII GGWCC 6 cut(s) 514, 1015, 2037, 2222, 2950, 3047
AxyI CCTNAGG 1 cut(s) 1401
BaeGI GKGCMC 1 cut(s) 1989
BaeI ACNNNNGTAYC 6 cut(s) 942, 975, 1149, 1182, 2637, 2670
BanI GGYRCC 2 cut(s) 1721, 1986
BanII GRGCYC 3 cut(s) 309, 2911, 3095
BarI GAAGNNNNNNTAC 2 cut(s) 2764, 2796
BauI CACGAG 2 cut(s) 1965, 2910
BbrPI CACGTG 1 cut(s) 548
BbsI GAAGAC 6 cut(s) 17, 48, 442, 564, 1797, 2984
Bbv12I GWGCWC 3 cut(s) 240, 309, 1560
BceAI ACGGC 3 cut(s) 712, 1249, 1950
BcgI CGANNNNNNTGC 4 cut(s) 278, 312, 3050, 3084
BciT130I CCWGG 6 cut(s) 111, 512, 1624, 2048, 2356, 3087
BciVI GTATCC 2 cut(s) 1628, 1872
BclI TGATCA 2 cut(s) 2661, 2973
BcnI CCSGG 2 cut(s) 518, 1420
BcoDI GTCTC 5 cut(s) 12, 586, 868, 875, 1532
BfaI CTAG 4 cut(s) 857, 1011, 1170, 1910
BfmI CTRYAG 3 cut(s) 426, 1563, 2022
BfoI RGCGCY 1 cut(s) 2807
BfrI CTTAAG 1 cut(s) 1829
BfuAI ACCTGC 2 cut(s) 640, 1719
BfuI GTATCC 2 cut(s) 1628, 1872
BmcAI AGTACT 2 cut(s) 1007, 1958
Bme1390I CCNGG 8 cut(s) 111, 512, 518, 1420, 1624, 2048, 2356, 3087
Bme18I GGWCC 6 cut(s) 514, 1015, 2037, 2222, 2950, 3047
BmeT110I CYCGRG 2 cut(s) 878, 3033
BmgT120I GGNCC 7 cut(s) 514, 1015, 1790, 2037, 2222, 2950, 3047
BmiI GGNNCC 9 cut(s) 516, 934, 1016, 1017, 1723, 1988, 2039, 2224, 3092
BmrFI CCNGG 8 cut(s) 111, 512, 518, 1420, 1624, 2048, 2356, 3087
BmrI ACTGGG 2 cut(s) 1158, 2340
BmsI GCATC 9 cut(s) 910, 1165, 1210, 1251, 2062, 2086, 2107, 2524, 3019
BmuI ACTGGG 2 cut(s) 1158, 2340
BpiI GAAGAC 6 cut(s) 17, 48, 442, 564, 1797, 2984
BplI GAGNNNNNCTC 2 cut(s) 2307, 2339
BpmI CTGGAG 6 cut(s) 312, 408, 2121, 2537, 2634, 2688
Bpu10I CCTNAGC 1 cut(s) 1746
Bpu14I TTCGAA 1 cut(s) 28
BpuEI CTTGAG 2 cut(s) 215, 2309
BpuMI CCSGG 2 cut(s) 518, 1420
BsaAI YACGTR 1 cut(s) 548
BsaBI GATNNNNATC 1 cut(s) 3072
BsaHI GRCGYC 2 cut(s) 1764, 1946
BsaI GGTCTC 3 cut(s) 12, 868, 875
BsaJI CCNNGG 8 cut(s) 420, 669, 1305, 1622, 1623, 2355, 2916, 3086
BsaWI WCCGGW 2 cut(s) 263, 693
BsaXI ACNNNNNCTCC 8 cut(s) 7, 37, 747, 777, 1302, 1332, 2094, 2124
Bsc4I CCNNNNNNNGG 8 cut(s) 110, 166, 517, 548, 719, 2047, 2404, 3086
Bse1I ACTGG 8 cut(s) 329, 1054, 1153, 1964, 2335, 2651, 2671, 2689
Bse21I CCTNAGG 1 cut(s) 1401
Bse3DI GCAATG 3 cut(s) 256, 1686, 2268
Bse8I GATNNNNATC 1 cut(s) 3072
BseBI CCWGG 6 cut(s) 111, 512, 1624, 2048, 2356, 3087
BseDI CCNNGG 8 cut(s) 420, 669, 1305, 1622, 1623, 2355, 2916, 3086
BseGI GGATG 9 cut(s) 587, 961, 1128, 1633, 2119, 2365, 2428, 2525, 2893
BseJI GATNNNNATC 1 cut(s) 3072
BseLI CCNNNNNNNGG 8 cut(s) 110, 166, 517, 548, 719, 2047, 2404, 3086
BseMI GCAATG 3 cut(s) 256, 1686, 2268
BseMII CTCAG 3 cut(s) 1392, 1737, 2277
BseNI ACTGG 8 cut(s) 329, 1054, 1153, 1964, 2335, 2651, 2671, 2689
BseRI GAGGAG 2 cut(s) 768, 1418
BseSI GKGCMC 1 cut(s) 1989
BseYI CCCAGC 1 cut(s) 2384
BsgI GTGCAG 3 cut(s) 753, 1352, 2447
Bsh1236I CGCG 1 cut(s) 2434
Bsh1285I CGRYCG 1 cut(s) 1424
BshFI GGCC 6 cut(s) 419, 1068, 1791, 1963, 2524, 2637
BshNI GGYRCC 2 cut(s) 1721, 1986
BsiEI CGRYCG 1 cut(s) 1424
BsiHKAI GWGCWC 3 cut(s) 240, 309, 1560
BsiHKCI CYCGRG 2 cut(s) 878, 3033
BsiSI CCGG 5 cut(s) 264, 441, 518, 694, 1420
BslFI GGGAC 5 cut(s) 183, 1001, 1402, 1624, 2208
BslI CCNNNNNNNGG 8 cut(s) 110, 166, 517, 548, 719, 2047, 2404, 3086
BsmAI GTCTC 5 cut(s) 12, 586, 868, 875, 1532
BsmBI CGTCTC 1 cut(s) 586
BsmFI GGGAC 5 cut(s) 183, 1001, 1402, 1624, 2208
BsmI GAATGC 3 cut(s) 176, 273, 1091
BsnI GGCC 6 cut(s) 419, 1068, 1791, 1963, 2524, 2637
Bso31I GGTCTC 3 cut(s) 12, 868, 875
BsoBI CYCGRG 2 cut(s) 878, 3033
Bsp119I TTCGAA 1 cut(s) 28
Bsp1286I GDGCHC 6 cut(s) 240, 309, 1560, 1989, 2911, 3095
Bsp1407I TGTACA 1 cut(s) 2275
BspACI CCGC 6 cut(s) 207, 285, 438, 1373, 2158, 2379
BspANI GGCC 6 cut(s) 419, 1068, 1791, 1963, 2524, 2637
BspCNI CTCAG 3 cut(s) 1393, 1738, 2278
BspFNI CGCG 1 cut(s) 2434
BspHI TCATGA 2 cut(s) 739, 2956
BspLI GGNNCC 9 cut(s) 516, 934, 1016, 1017, 1723, 1988, 2039, 2224, 3092
BspMAI CTGCAG 1 cut(s) 430
BspMI ACCTGC 2 cut(s) 640, 1719
BspPI GGATC 2 cut(s) 835, 1891
BspT104I TTCGAA 1 cut(s) 28
BspT107I GGYRCC 2 cut(s) 1721, 1986
BspTI CTTAAG 1 cut(s) 1829
BspTNI GGTCTC 3 cut(s) 12, 868, 875
BsrDI GCAATG 3 cut(s) 256, 1686, 2268
BsrGI TGTACA 1 cut(s) 2275
BsrI ACTGG 8 cut(s) 329, 1054, 1153, 1964, 2335, 2651, 2671, 2689
BssECI CCNNGG 8 cut(s) 420, 669, 1305, 1622, 1623, 2355, 2916, 3086
BssNI GRCGYC 2 cut(s) 1764, 1946
BssSI CACGAG 2 cut(s) 1965, 2910
BssT1I CCWWGG 3 cut(s) 420, 669, 2916
Bst2BI CACGAG 2 cut(s) 1965, 2910
Bst2UI CCWGG 6 cut(s) 111, 512, 1624, 2048, 2356, 3087
Bst4CI ACNGT 8 cut(s) 23, 57, 315, 494, 1306, 1653, 2399, 2762
Bst6I CTCTTC 2 cut(s) 57, 1497
BstACI GRCGYC 2 cut(s) 1764, 1946
BstAFI CTTAAG 1 cut(s) 1829
BstAPI GCANNNNNTGC 1 cut(s) 1993
BstAUI TGTACA 1 cut(s) 2275
BstBAI YACGTR 1 cut(s) 548
BstBI TTCGAA 1 cut(s) 28
BstC8I GCNNGC 1 cut(s) 1726
BstDEI CTNAG 6 cut(s) 221, 924, 1401, 1639, 1746, 2286
BstDSI CCRYGG 1 cut(s) 1305
BstENI CCTNNNNNAGG 1 cut(s) 2045
BstF5I GGATG 9 cut(s) 587, 961, 1128, 1633, 2119, 2365, 2428, 2525, 2893
BstFNI CGCG 1 cut(s) 2434
BstH2I RGCGCY 1 cut(s) 2807
BstHHI GCGC 1 cut(s) 2806
BstMAI GTCTC 5 cut(s) 12, 586, 868, 875, 1532
BstMCI CGRYCG 1 cut(s) 1424
BstMWI GCNNNNNNNGC 7 cut(s) 371, 425, 437, 1515, 1993, 2147, 2906
BstNI CCWGG 6 cut(s) 111, 512, 1624, 2048, 2356, 3087
BstNSI RCATGY 3 cut(s) 176, 2311, 3008
BstSCI CCNGG 8 cut(s) 109, 510, 516, 1418, 1622, 2046, 2354, 3085
BstSFI CTRYAG 3 cut(s) 426, 1563, 2022
BstSLI GKGCMC 1 cut(s) 1989
BstUI CGCG 1 cut(s) 2434
BstV2I GAAGAC 6 cut(s) 17, 48, 442, 564, 1797, 2984
BstXI CCANNNNNNTGG 1 cut(s) 317
Bsu36I CCTNAGG 1 cut(s) 1401
BsuI GTATCC 2 cut(s) 1628, 1872
BsuRI GGCC 6 cut(s) 419, 1068, 1791, 1963, 2524, 2637
BtgI CCRYGG 1 cut(s) 1305
BtsCI GGATG 9 cut(s) 587, 961, 1128, 1633, 2119, 2365, 2428, 2525, 2893
BtsI GCAGTG 1 cut(s) 3096
BveI ACCTGC 2 cut(s) 640, 1719
Cac8I GCNNGC 1 cut(s) 1726
CciI TCATGA 2 cut(s) 739, 2956
CfoI GCGC 1 cut(s) 2806
Cfr13I GGNCC 7 cut(s) 514, 1015, 1790, 2037, 2222, 2950, 3047
CseI GACGC 3 cut(s) 53, 2423, 2440
CspCI CAANNNNNGTGG 2 cut(s) 38, 73
DdeI CTNAG 6 cut(s) 221, 924, 1401, 1639, 1746, 2286
DraI TTTAAA 1 cut(s) 1209
DraIII CACNNNGTG 1 cut(s) 548
Eam1104I CTCTTC 2 cut(s) 57, 1497
EarI CTCTTC 2 cut(s) 57, 1497
EciI GGCGGA 1 cut(s) 427
Ecl136II GAGCTC 1 cut(s) 307
Eco130I CCWWGG 3 cut(s) 420, 669, 2916
Eco24I GRGCYC 3 cut(s) 309, 2911, 3095
Eco31I GGTCTC 3 cut(s) 12, 868, 875
Eco32I GATATC 1 cut(s) 501
Eco47I GGWCC 6 cut(s) 514, 1015, 2037, 2222, 2950, 3047
Eco53kI GAGCTC 1 cut(s) 307
Eco57I CTGAAG 4 cut(s) 1521, 2184, 2523, 2873
Eco72I CACGTG 1 cut(s) 548
Eco81I CCTNAGG 1 cut(s) 1401
Eco88I CYCGRG 2 cut(s) 878, 3033
EcoICRI GAGCTC 1 cut(s) 307
EcoNI CCTNNNNNAGG 1 cut(s) 2045
EcoO109I RGGNCCY 5 cut(s) 514, 1015, 2037, 2222, 2950
EcoRI GAATTC 2 cut(s) 1313, 1634
EcoRII CCWGG 6 cut(s) 109, 510, 1622, 2046, 2354, 3085
EcoRV GATATC 1 cut(s) 501
EcoT14I CCWWGG 3 cut(s) 420, 669, 2916
EcoT22I ATGCAT 2 cut(s) 178, 907
EcoT38I GRGCYC 3 cut(s) 309, 2911, 3095
ErhI CCWWGG 3 cut(s) 420, 669, 2916
Esp3I CGTCTC 1 cut(s) 586
FaqI GGGAC 5 cut(s) 183, 1001, 1402, 1624, 2208
FauI CCCGC 1 cut(s) 1366
FauNDI CATATG 1 cut(s) 1471
FbaI TGATCA 2 cut(s) 2661, 2973
FblI GTMKAC 1 cut(s) 2021
FokI GGATG 9 cut(s) 594, 968, 1115, 1640, 2126, 2372, 2435, 2532, 2900
FriOI GRGCYC 3 cut(s) 309, 2911, 3095
FspBI CTAG 4 cut(s) 857, 1011, 1170, 1910
GlaI GCGC 1 cut(s) 2805
GsaI CCCAGC 1 cut(s) 2388
GsuI CTGGAG 6 cut(s) 312, 408, 2121, 2537, 2634, 2688
HaeII RGCGCY 1 cut(s) 2807
HaeIII GGCC 6 cut(s) 419, 1068, 1791, 1963, 2524, 2637
HapII CCGG 5 cut(s) 264, 441, 518, 694, 1420
HgaI GACGC 3 cut(s) 53, 2423, 2440
HhaI GCGC 1 cut(s) 2806
Hin1I GRCGYC 2 cut(s) 1764, 1946
Hin6I GCGC 1 cut(s) 2804
HinP1I GCGC 1 cut(s) 2804
HincII GTYRAC 1 cut(s) 1125
HindII GTYRAC 1 cut(s) 1125
HindIII AAGCTT 3 cut(s) 191, 567, 2600
HinfI GANTC 7 cut(s) 1540, 1576, 1612, 1646, 1658, 2204, 3073
HpaII CCGG 5 cut(s) 264, 441, 518, 694, 1420
HphI GGTGA 5 cut(s) 172, 1074, 1270, 1439, 2015
Hpy166II GTNNAC 5 cut(s) 699, 1125, 1592, 2022, 2278
Hpy8I GTNNAC 5 cut(s) 699, 1125, 1592, 2022, 2278
HpyCH4III ACNGT 8 cut(s) 23, 57, 315, 494, 1306, 1653, 2399, 2762
HpyCH4IV ACGT 5 cut(s) 547, 595, 869, 1764, 1946
HpyF10VI GCNNNNNNNGC 7 cut(s) 371, 425, 437, 1515, 1993, 2147, 2906
HpyF3I CTNAG 6 cut(s) 221, 924, 1401, 1639, 1746, 2286
HpySE526I ACGT 5 cut(s) 547, 595, 869, 1764, 1946
Hsp92I GRCGYC 2 cut(s) 1764, 1946
HspAI GCGC 1 cut(s) 2804
KflI GGGWCCC 1 cut(s) 1015
Ksp22I TGATCA 2 cut(s) 2661, 2973
LmnI GCTCC 6 cut(s) 312, 389, 932, 1183, 1405, 3090
LweI GCATC 9 cut(s) 910, 1165, 1210, 1251, 2062, 2086, 2107, 2524, 3019
MaeI CTAG 4 cut(s) 857, 1011, 1170, 1910
MaeII ACGT 5 cut(s) 547, 595, 869, 1764, 1946
MaeIII GTNAC 7 cut(s) 1080, 1156, 1647, 2134, 2653, 2762, 2825
MfeI CAATTG 2 cut(s) 2052, 2895
MhlI GDGCHC 6 cut(s) 240, 309, 1560, 1989, 2911, 3095
MluI ACGCGT 1 cut(s) 2432
MlyI GAGTC 4 cut(s) 1534, 1606, 1655, 2198
MmeI TCCRAC 2 cut(s) 334, 1974
Mph1103I ATGCAT 2 cut(s) 178, 907
MroXI GAANNNNTTC 6 cut(s) 122, 155, 536, 623, 762, 851
MslI CAYNNNNRTG 4 cut(s) 266, 344, 791, 2712
MspCI CTTAAG 1 cut(s) 1829
MspI CCGG 5 cut(s) 264, 441, 518, 694, 1420
MspR9I CCNGG 8 cut(s) 111, 512, 518, 1420, 1624, 2048, 2356, 3087
MunI CAATTG 2 cut(s) 2052, 2895
Mva1269I GAATGC 3 cut(s) 176, 273, 1091
MvaI CCWGG 6 cut(s) 111, 512, 1624, 2048, 2356, 3087
MvnI CGCG 1 cut(s) 2434
MwoI GCNNNNNNNGC 7 cut(s) 371, 425, 437, 1515, 1993, 2147, 2906
NciI CCSGG 2 cut(s) 518, 1420
NdeI CATATG 1 cut(s) 1471
NlaIV GGNNCC 9 cut(s) 516, 934, 1016, 1017, 1723, 1988, 2039, 2224, 3092
NmeAIII GCCGAG 1 cut(s) 1522
NmuCI GTSAC 6 cut(s) 1080, 1156, 1647, 2134, 2762, 2825
NsiI ATGCAT 2 cut(s) 178, 907
NspI RCATGY 3 cut(s) 176, 2311, 3008
NspV TTCGAA 1 cut(s) 28
PagI TCATGA 2 cut(s) 739, 2956
PasI CCCWGGG 1 cut(s) 1623
PciI ACATGT 1 cut(s) 2307
PctI GAATGC 3 cut(s) 176, 273, 1091
PdmI GAANNNNTTC 6 cut(s) 122, 155, 536, 623, 762, 851
PfeI GAWTC 3 cut(s) 1576, 1658, 3073
PflFI GACNNNGTC 1 cut(s) 2246
PflMI CCANNNNNTGG 2 cut(s) 548, 719
PfoI TCCNGGA 1 cut(s) 510
PleI GAGTC 4 cut(s) 1534, 1606, 1654, 2198
PmaCI CACGTG 1 cut(s) 548
PmlI CACGTG 1 cut(s) 548
PpsI GAGTC 4 cut(s) 1534, 1606, 1654, 2198
Ppu21I YACGTR 1 cut(s) 548
PpuMI RGGWCCY 5 cut(s) 514, 1015, 2037, 2222, 2950
PscI ACATGT 1 cut(s) 2307
Psp124BI GAGCTC 1 cut(s) 309
Psp1406I AACGTT 1 cut(s) 869
Psp5II RGGWCCY 5 cut(s) 514, 1015, 2037, 2222, 2950
Psp6I CCWGG 6 cut(s) 109, 510, 1622, 2046, 2354, 3085
PspCI CACGTG 1 cut(s) 548
PspFI CCCAGC 1 cut(s) 2384
PspGI CCWGG 6 cut(s) 109, 510, 1622, 2046, 2354, 3085
PspN4I GGNNCC 9 cut(s) 516, 934, 1016, 1017, 1723, 1988, 2039, 2224, 3092
PspPI GGNCC 7 cut(s) 514, 1015, 1790, 2037, 2222, 2950, 3047
PspPPI RGGWCCY 5 cut(s) 514, 1015, 2037, 2222, 2950
PstI CTGCAG 1 cut(s) 430
PsyI GACNNNGTC 1 cut(s) 2246
RseI CAYNNNNRTG 4 cut(s) 266, 344, 791, 2712
SacI GAGCTC 1 cut(s) 309
Sau96I GGNCC 7 cut(s) 514, 1015, 1790, 2037, 2222, 2950, 3047
ScaI AGTACT 2 cut(s) 1007, 1958
SchI GAGTC 4 cut(s) 1534, 1606, 1655, 2198
ScrFI CCNGG 8 cut(s) 111, 512, 518, 1420, 1624, 2048, 2356, 3087
SduI GDGCHC 6 cut(s) 240, 309, 1560, 1989, 2911, 3095
SfaNI GCATC 9 cut(s) 910, 1165, 1210, 1251, 2062, 2086, 2107, 2524, 3019
SfcI CTRYAG 3 cut(s) 426, 1563, 2022
SfuI TTCGAA 1 cut(s) 28
SinI GGWCC 6 cut(s) 514, 1015, 2037, 2222, 2950, 3047
SmiMI CAYNNNNRTG 4 cut(s) 266, 344, 791, 2712
SmlI CTYRAG 3 cut(s) 194, 1829, 2324
SmoI CTYRAG 3 cut(s) 194, 1829, 2324
SsiI CCGC 6 cut(s) 207, 285, 438, 1373, 2158, 2379
SspI AATATT 1 cut(s) 1856
SspMI CTAG 4 cut(s) 857, 1011, 1170, 1910
SstI GAGCTC 1 cut(s) 309
StyD4I CCNGG 8 cut(s) 109, 510, 516, 1418, 1622, 2046, 2354, 3085
StyI CCWWGG 3 cut(s) 420, 669, 2916
TaaI ACNGT 8 cut(s) 23, 57, 315, 494, 1306, 1653, 2399, 2762
TaiI ACGT 5 cut(s) 550, 598, 872, 1767, 1949
TaqI TCGA 4 cut(s) 28, 717, 1041, 2842
TaqII GACCGA 1 cut(s) 2978
TatI WGTACW 4 cut(s) 1005, 1956, 2275, 2364
TauI GCSGC 3 cut(s) 209, 287, 2161
TfiI GAWTC 3 cut(s) 1576, 1658, 3073
TseFI GTSAC 6 cut(s) 1080, 1156, 1647, 2134, 2762, 2825
Tsp45I GTSAC 6 cut(s) 1080, 1156, 1647, 2134, 2762, 2825
TspGWI ACGGA 2 cut(s) 968, 3069
Tth111I GACNNNGTC 1 cut(s) 2246
Van91I CCANNNNNTGG 2 cut(s) 548, 719
Vha464I CTTAAG 1 cut(s) 1829
VpaK11BI GGWCC 6 cut(s) 514, 1015, 2037, 2222, 2950, 3047
XagI CCTNNNNNAGG 1 cut(s) 2045
XapI RAATTY 7 cut(s) 130, 821, 1313, 1634, 2060, 2122, 2748
XceI RCATGY 3 cut(s) 176, 2311, 3008
XmiI GTMKAC 1 cut(s) 2021
XmnI GAANNNNTTC 6 cut(s) 122, 155, 536, 623, 762, 851
XspI CTAG 4 cut(s) 857, 1011, 1170, 1910
ZraI GACGTC 2 cut(s) 1765, 1947
ZrmI AGTACT 2 cut(s) 1007, 1958
Zsp2I ATGCAT 2 cut(s) 178, 907
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.