RchiOBHm_Chr1g0336121

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000598
Physical Location & Seq
Forward (+)
27813339 .. 27818335
4997 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56410

Sequence Viewer

Length: 3279 bp
ATGGCGGAGGAAGGCGGCAAGAGCTTTGCCAGGAGAGACCATCTCCTAGAGATTGAGGCAAAGGCTCGAAGCTGGTGGGAAGACAAACAAGTTTTCAGGGCCCAGTCTCGTGCAGAGCCGCCCGGCCCAGGGGAGAAGTTCTTTGGAAACTTCCCCTTCCCTTATATGAACGGTTATCTGCATCTCGGACATGCCTTCTCACTGTCGAAGCTGGAGTTTGCTGCAGCCTATCATCGATTGAGAGGCGCAAATGTCCTCTTGCCTTTTGCTTTTCACTGTACTGGCATGCCCATCAAAGCCTCTGCCGATAAGCTTGTTAGGGAGATTCAACAGTTTGGAGAACCTCCTGTTTTTCCCAAACAAGTGGAGAAAGGGAACGAGGAAGTGGAAGTGGAAGCAGAGGATGTCAATGCAGGCGAACCTCCAGCCAAGCATAAGGGAAAGAAGTCAAAGGCTGCATCAAAATCAAGCGGCGAAGTGTACCAATGGGAAATTATGCAGAGCTTTGGCCTCTCCGACAGTGACATATCCAAATTTCAGGACCCTTATAAGTGGTTGACTTATTTTCCCCCATTGGCTGTGGAGGACCTCAAGGCTTTTGGCCTGGGCTGTGACTGGAGACGTTCCTTTATTACTACAGACATGAACCCTTTTTTCGACGGCTTTGTGAGGTGGCAGGTTAGGAAACTGAAATCCATGGGTAAGATTGTCAAAGATCTCCGTTACACGATATACTCTCCGATGGATGGGCAACCCTGTGCAGATCATGATAGGTCCAGTGGTGAAGGAGTTCAGCCCCAAGAATATACTGTCATCAAAATGGAACTGATGGCACCTTTTCCTTCTAAATTGGGAGTGTTGGAGGGGAGGAAAGTGTTCCTTGCTGCAGCAACACTGAGACCTGAGACCATGTATGGACAAACAAATGCATGGGTATTGCCTGATGGTAAGTATGGTGCATATGAAGTCAATGAAACAGATGTGTTCATTCTTACACAGAGAGCAGCACGTAATCTTGCTTATCAAAAGTACTCTAGGGTCCCAGAGAAACCTACTTGCTTGGTTGAACTGACTGGTTACGATTTGATTGGCCTTCCATTGAAGTCGCCACTAGCAGTGAATCAGATTATTTATGCTCTTCCTATGTTGACTGTCCTAACAGACAAAGGTACTGGGATTGTGACCAGTGTACCTACTGATTCTCCGGATGACTATATGGCCTTGCATGACTTGAAATCAAAACCTGCTTTTAGGGCAAAATTTGGTGTGAAAGATGAGTGGGTACTGCCCTTTGAGATTATCCCAATCATCGATATTCCAGGATATGGAAATAAGGCTGCGGAAAAGGTCTGTGCAGATCTCAAGATCAAAAGCCAGAATGAGAAAGAGAAGCTAGCAGAAGCCAAGAGGTTGACATACTTGAAAGGATTCAATGAAGGAACACTGATTGTGGGGGAATTTAAGGGAAGGAAAGTCCAGGAGGTTAAACCCTTGATAAGGAGCAAGCTTATAGAGGCAGGGGAGGCAATATCATATAGTGAACCTGAGAAGCGGGTACTATCACGATCAGGTGATGAATGTGTTGTGGCGCTTACAGATCAATGGTACATCACTTATGGGGAAACTGAATGGAAAAAATTGGCTGAGGAGTGCTTGTCCGGCATGAATTTGTATTCTGATGAGACACGGCATGGATTTGAACACACATTGAGCTGGTTGAATCAGTGGGCTTGTTCACGATCTTTTGGTCTTGGGACTCGCATCCCCTGGGATGAAGAATTCTTAGTTGAGTCATTGTCTGATTCAACTATTTACATGGCTTATTACACCATTGCTCACTTCCTACACAACGGAGACATGTATGGTTCCAGCAAATCTGCAATTAAACCTGAACAAATGACTGATGAGGTCTGGGATTTTATTTTCTGTGACGGCCCATATCCTAAATCATCCGACATCTCATCATCAATTCTGAGTGAGATGAAGCAGGAGTTTGAATATTGGTATCCTTTTGATCTTCGGGTGTCTGGCAAGGATCTAATACAAAATCATTTAACCTTCTGCATCTACAACCATACAGCTATTATGTCCAAGAAGCACTGGCCTCGTGGTTTCAGATGCAATGGGCACATAATGCTCAATGCTCAGAAAATGTCCAAGTCCACAGGAAACTTTAGGACAATTCGCCAGGCAATTGTGGAATTTTCAGCTGATGCCACTCGCTTTTCTTTGGCTGATGCTGGAGATGGTGTTGATGATGCAAATTTTGCATTTGACACTGCCAATGCTGCAATCCTGCGGCTCACTAAAGAGATCTCATGGATGGAAGAAGTTTTAGGTGCAAACTCTTCTTTGAGACCAGGACCCCCAACTACTTATGCTGACAGGGTCTTTGCGAATGAGATAAATATTGCTGTGAACAGGACTGAGCAGAATTACAGCGATTACATGTTCCGAGAAGCCCTCAAGACTGGATTTTATGATCTTCAGGCTGCTAGGGATGAGTATCGGTTTTCATGTGGTGCTGGGGGAATGAATCGTGATTTGGTGTGGCGTTTTATGGATGTGCAGACACGTCTTATTACTCCAATCTGCCCACACTACGGAGAATACGTATGGAGGGAACTTTTGAAGAAGGATGGCTTTGTGGTAAACGCAGGATGGCCTTTGGCTGACGCTCCGGACCTAACCCTCCAGAGTGCCAATAAATATTTGCAGGACTCAATCATTTCAATGAGGAAGCTCCTCCAAAAGCAAGTTTCAAGCTCAAAGAAGGGTAAGAAGGGTACTCCAGTCACAGTGTTGACAGAAGACAACAATCTGACCGGCTTAATCTATGTGAACGAGCACTTTGATGGATGGAAGGCAGAGTGCCTGAAAATCCTCCAAATTAACTTTAACAGAGACAGTCGTACTTTTTCTTCAGACAAGATGATACAAGAAGAGTTGCAAAAAAGTTCTATTGGTCAGTCGAAAGATTTTAGACAAATCCAAAAGCTTTGTATGCCTTTCATGAAGTTCAAGAAGGATGAGGCAATTGCAATTGGGGCTCAGGCCTTGGATTTGAAGCTGCCTTTTGGTGAGATTGAGGTCCTTCAAGAGAACTTGGACTTAATTAAGAGACAACTTGGTCTTCAAGAGGTAGAAATATTGTCTGCTACAAATCCTGATGCACTTTCTAAAGCCGGTTCGCTTGTCAAACTGTTAGAGCAGAATCCTCCAGCACCCGGAAGCCCTACTGCTATCTTCTTGACCTGTTCTCTTCACTACCATCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

1092

Amino Acids

123.52

Weight (kDa)

6.15

Isoelectric Point (pI)

45.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1 PF00133 24 - 106 6.1e-08 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1 PF00133 193 - 755 8.4e-16 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g PF09334 663 - 761 1.4e-07 tRNA synthetases class I (M)
tRNA-synt_1e PF01406 669 - 745 2.9e-08 tRNA synthetases class I (C) catalytic domain
Anticodon_1 PF08264 795 - 919 6.8e-13 Anticodon-binding domain of tRNA ligase
RBD_LARS1 PF24810 948 - 1024 1.4e-06 Leucine--tRNA ligase, RagD-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000598)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09620
fragaria_vesca FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_3g34581 FvH4_4g33720 FvH4_4g33720
malus_domestica MD08G1244600.v1.1 MD15G1439400.v1.1
prunus_persica Prupe.1G582000_v2.0.a1 Prupe.2G015100_v2.0.a1 Prupe.2G123100_v2.0.a1
pyrus_communis pycom08g21210 pycom08g21220 pycom15g38730
rosa_chinensis RchiOBHm_Chr1g0336121 RchiOBHm_Chr1g0353811 RchiOBHm_Chr1g0373331 RchiOBHm_Chr4g0443191 RchiOBHm_Chr4g0443201 RchiOBHm_Chr4g0443211
rosa_laevigata RLG00000003122 RLG00000005948 RLG00000026826 RLG00000028251 RLG00000028252 RLG00000028253 RLG00000029377
rosa_multiflora Rmu_co8002498.1_g000001 Rmu_co8262279.1_g000001 Rmu_co8314139.1_g000001 Rmu_co8393711.1_g000001 Rmu_co8419167.1_g000001 Rmu_sc0007968.1_g000004 Rmu_sc0018919.1_g000004 Rmu_sc0027447.1_g000001
rosa_roxburghii Rroxscaffold_4G00284260 Rroxscaffold_4G00301490 Rroxscaffold_4G00315860 Rroxscaffold_5G00383790 Rroxscaffold_5G00383800
rosa_rugosa Rorug01G0123100 Rorug01G0238700 Rorug01G0377100 Rorug01G0377100 Rorug01G0377200 Rorug01G0377300 Rorug04G0344700 Rorug05G0347900.1
rosa_samantha Rh1AG143800 Rh1AG250300 Rh1AG386300 Rh1BG113400 Rh1BG220800 Rh1BG220900 Rh1BG350400 Rh1CG135500 Rh1CG233100 Rh1CG363500 Rh1DG150200 Rh1DG247700 Rh1DG247800 Rh1DG381300 Rh4AG399400 Rh4AG399500 Rh4BG411000 Rh4BG411100 Rh4BG411200 Rh4CG426500 Rh4CG426600 Rh4DG406100 Rh4DG406200 Rh4DG406300
rosa_wichuraiana Rw0G015190 Rw1G021850 Rw1G034870 Rw4G034440 Rw4G034450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 549
AasI GACNNNNNNGTC 1 cut(s) 3129
Acc36I ACCTGC 2 cut(s) 667, 1252
AccB1I GGYRCC 1 cut(s) 832
AccB7I CCANNNNNTGG 1 cut(s) 1325
AccIII TCCGGA 2 cut(s) 1204, 2680
AciI CCGC 7 cut(s) 5, 15, 119, 471, 1340, 1552, 2299
AclWI GGATC 1 cut(s) 2043
AcsI RAATTY 7 cut(s) 533, 1259, 1457, 1666, 1778, 2201, 2263
AcuI CTGAAG 2 cut(s) 2471, 2907
AfiI CCNNNNNNNGG 7 cut(s) 128, 129, 746, 1325, 1497, 2603, 3227
AflIII ACRYGT 3 cut(s) 1857, 2448, 2573
AjiI CACGTC 1 cut(s) 2576
AjnI CCWGG 8 cut(s) 29, 127, 603, 1318, 1476, 1766, 2187, 2359
AjuI GAANNNNNNNTTGG 2 cut(s) 2528, 2560
Alw21I GWGCWC 1 cut(s) 2850
AlwI GGATC 1 cut(s) 2043
Aor13HI TCCGGA 2 cut(s) 1204, 2680
ApaI GGGCCC 1 cut(s) 103
ApoI RAATTY 7 cut(s) 533, 1259, 1457, 1666, 1778, 2201, 2263
ArsI GACNNNNNNTTYG 2 cut(s) 2375, 2407
Asp700I GAANNNNTTC 3 cut(s) 789, 875, 1427
AspLEI GCGC 2 cut(s) 248, 1591
AsuC2I CCSGG 2 cut(s) 123, 3228
AsuHPI GGTGA 3 cut(s) 794, 1583, 3092
AsuNHI GCTAGC 1 cut(s) 1393
AvaII GGWCC 7 cut(s) 541, 586, 774, 1039, 2363, 2683, 3091
BaeGI GKGCMC 2 cut(s) 103, 2130
BaeI ACNNNNGTAYC 4 cut(s) 1173, 1206, 2778, 2811
BanI GGYRCC 1 cut(s) 832
BanII GRGCYC 2 cut(s) 103, 3052
BarI GAAGNNNNNNTAC 2 cut(s) 2905, 2937
BauI CACGAG 2 cut(s) 108, 2106
BbsI GAAGAC 3 cut(s) 87, 2817, 3125
Bbv12I GWGCWC 1 cut(s) 2850
BbvCI CCTCAGC 1 cut(s) 1644
BceAI ACGGC 3 cut(s) 676, 1703, 1948
BcgI CGANNNNNNTGC 2 cut(s) 2087, 2121
BciT130I CCWGG 8 cut(s) 31, 129, 605, 1320, 1478, 1768, 2189, 2361
BciVI GTATCC 1 cut(s) 2016
BcnI CCSGG 2 cut(s) 123, 3228
BfaI CTAG 5 cut(s) 47, 1035, 1112, 1394, 2496
BfmI CTRYAG 3 cut(s) 222, 636, 885
BfoI RGCGCY 1 cut(s) 1592
BfuAI ACCTGC 2 cut(s) 667, 1252
BfuI GTATCC 1 cut(s) 2016
BglII AGATCT 3 cut(s) 715, 1357, 2313
BmcAI AGTACT 1 cut(s) 1031
Bme18I GGWCC 7 cut(s) 541, 586, 774, 1039, 2363, 2683, 3091
BmgBI CACGTC 1 cut(s) 2576
BmiI GGNNCC 7 cut(s) 101, 543, 834, 1040, 1041, 1867, 2365
BmrI ACTGGG 2 cut(s) 97, 1182
BmsI GCATC 9 cut(s) 190, 467, 1770, 2073, 2108, 2203, 2227, 2248, 3160
BmtI GCTAGC 1 cut(s) 1397
BmuI ACTGGG 2 cut(s) 97, 1182
BpiI GAAGAC 3 cut(s) 87, 2817, 3125
BplI GAGNNNNNCTC 4 cut(s) 27, 59, 2448, 2480
BpmI CTGGAG 7 cut(s) 233, 408, 637, 2262, 2678, 2775, 3204
Bpu10I CCTNAGC 2 cut(s) 1644, 3051
BpuEI CTTGAG 3 cut(s) 575, 1346, 2450
BpuMI CCSGG 2 cut(s) 123, 3228
Bsa29I ATCGAT 2 cut(s) 235, 1311
BsaAI YACGTR 2 cut(s) 1010, 2614
BsaBI GATNNNNATC 1 cut(s) 2505
BsaI GGTCTC 4 cut(s) 30, 892, 899, 2350
BsaJI CCNNGG 7 cut(s) 127, 128, 604, 696, 1766, 1767, 3057
BsaWI WCCGGW 2 cut(s) 1204, 2680
BsaXI ACNNNNNCTCC 8 cut(s) 1186, 1216, 1640, 1670, 2235, 2265, 2598, 2628
Bsc4I CCNNNNNNNGG 7 cut(s) 128, 129, 746, 1325, 1497, 2603, 3227
Bse118I RCCGGY 2 cut(s) 2825, 3185
Bse3DI GCAATG 2 cut(s) 1830, 2128
Bse8I GATNNNNATC 1 cut(s) 2505
BseAI TCCGGA 2 cut(s) 1204, 2680
BseBI CCWGG 8 cut(s) 31, 129, 605, 1320, 1478, 1768, 2189, 2361
BseCI ATCGAT 2 cut(s) 235, 1311
BseDI CCNNGG 7 cut(s) 127, 128, 604, 696, 1766, 1767, 3057
BseJI GATNNNNATC 1 cut(s) 2505
BseLI CCNNNNNNNGG 7 cut(s) 128, 129, 746, 1325, 1497, 2603, 3227
BseMI GCAATG 2 cut(s) 1830, 2128
BseMII CTCAG 8 cut(s) 887, 894, 1536, 1635, 1964, 2159, 2418, 3065
BseRI GAGGAG 2 cut(s) 1661, 2735
BseSI GKGCMC 2 cut(s) 103, 2130
BseYI CCCAGC 1 cut(s) 2525
BsgI GTGCAG 4 cut(s) 132, 780, 1374, 2588
BshNI GGYRCC 1 cut(s) 832
BshVI ATCGAT 2 cut(s) 235, 1311
BsiHKAI GWGCWC 1 cut(s) 2850
BsiSI CCGG 7 cut(s) 123, 1205, 1659, 2681, 2826, 3186, 3228
BslFI GGGAC 2 cut(s) 1025, 1768
BslI CCNNNNNNNGG 7 cut(s) 128, 129, 746, 1325, 1497, 2603, 3227
BsmBI CGTCTC 1 cut(s) 613
BsmFI GGGAC 2 cut(s) 1025, 1768
Bso31I GGTCTC 4 cut(s) 30, 892, 899, 2350
Bsp120I GGGCCC 1 cut(s) 99
Bsp1286I GDGCHC 4 cut(s) 103, 2130, 2850, 3052
Bsp13I TCCGGA 2 cut(s) 1204, 2680
Bsp19I CCATGG 1 cut(s) 696
BspACI CCGC 7 cut(s) 5, 15, 119, 471, 1340, 1552, 2299
BspCNI CTCAG 8 cut(s) 888, 895, 1537, 1636, 1965, 2158, 2419, 3064
BspDI ATCGAT 2 cut(s) 235, 1311
BspEI TCCGGA 2 cut(s) 1204, 2680
BspHI TCATGA 2 cut(s) 766, 3012
BspLI GGNNCC 7 cut(s) 101, 543, 834, 1040, 1041, 1867, 2365
BspMAI CTGCAG 2 cut(s) 226, 889
BspMI ACCTGC 2 cut(s) 667, 1252
BspOI GCTAGC 1 cut(s) 1397
BspPI GGATC 1 cut(s) 2043
BspQI GCTCTTC 1 cut(s) 1143
BspT107I GGYRCC 1 cut(s) 832
BspTNI GGTCTC 4 cut(s) 30, 892, 899, 2350
BsrDI GCAATG 2 cut(s) 1830, 2128
BsrFI RCCGGY 2 cut(s) 2825, 3185
BssAI RCCGGY 2 cut(s) 2825, 3185
BssECI CCNNGG 7 cut(s) 127, 128, 604, 696, 1766, 1767, 3057
BssSI CACGAG 2 cut(s) 108, 2106
BssT1I CCWWGG 2 cut(s) 696, 3057
Bst2BI CACGAG 2 cut(s) 108, 2106
Bst2UI CCWGG 8 cut(s) 31, 129, 605, 1320, 1478, 1768, 2189, 2361
Bst6I CTCTTC 4 cut(s) 1143, 2353, 2937, 3267
BstAPI GCANNNNNTGC 2 cut(s) 2134, 2267
BstBAI YACGTR 2 cut(s) 1010, 2614
BstC8I GCNNGC 4 cut(s) 287, 415, 1395, 1505
BstDEI CTNAG 9 cut(s) 896, 903, 1545, 1644, 1783, 1973, 2145, 2427, 3051
BstDSI CCRYGG 1 cut(s) 696
BstENI CCTNNNNNAGG 1 cut(s) 1495
BstH2I RGCGCY 1 cut(s) 1592
BstHHI GCGC 2 cut(s) 248, 1591
BstMWI GCNNNNNNNGC 9 cut(s) 21, 1253, 1523, 1659, 2134, 2267, 2288, 3004, 3047
BstNI CCWGG 8 cut(s) 31, 129, 605, 1320, 1478, 1768, 2189, 2361
BstNSI RCATGY 4 cut(s) 194, 289, 1861, 2452
BstSFI CTRYAG 3 cut(s) 222, 636, 885
BstSLI GKGCMC 2 cut(s) 103, 2130
BstSNI TACGTA 1 cut(s) 2614
BstV2I GAAGAC 3 cut(s) 87, 2817, 3125
BstX2I RGATCY 4 cut(s) 715, 1357, 2035, 2313
BstXI CCANNNNNNTGG 1 cut(s) 364
BstYI RGATCY 4 cut(s) 715, 1357, 2035, 2313
Bsu15I ATCGAT 2 cut(s) 235, 1311
BsuI GTATCC 1 cut(s) 2016
BsuTUI ATCGAT 2 cut(s) 235, 1311
BtgI CCRYGG 1 cut(s) 696
BtrI CACGTC 1 cut(s) 2576
BtsI GCAGTG 2 cut(s) 1122, 2277
BveI ACCTGC 2 cut(s) 667, 1252
Cac8I GCNNGC 4 cut(s) 287, 415, 1395, 1505
CciI TCATGA 2 cut(s) 766, 3012
CfoI GCGC 2 cut(s) 248, 1591
Cfr10I RCCGGY 2 cut(s) 2825, 3185
ClaI ATCGAT 2 cut(s) 235, 1311
CseI GACGC 1 cut(s) 2684
DdeI CTNAG 9 cut(s) 896, 903, 1545, 1644, 1783, 1973, 2145, 2427, 3051
DrdI GACNNNNNNGTC 1 cut(s) 3129
DseDI GACNNNNNNGTC 1 cut(s) 3129
Eam1104I CTCTTC 4 cut(s) 1143, 2353, 2937, 3267
EarI CTCTTC 4 cut(s) 1143, 2353, 2937, 3267
EciI GGCGGA 1 cut(s) 20
Eco105I TACGTA 1 cut(s) 2614
Eco130I CCWWGG 2 cut(s) 696, 3057
Eco147I AGGCCT 1 cut(s) 3056
Eco24I GRGCYC 2 cut(s) 103, 3052
Eco31I GGTCTC 4 cut(s) 30, 892, 899, 2350
Eco47I GGWCC 7 cut(s) 541, 586, 774, 1039, 2363, 2683, 3091
Eco57I CTGAAG 2 cut(s) 2471, 2907
EcoNI CCTNNNNNAGG 1 cut(s) 1495
EcoO109I RGGNCCY 6 cut(s) 99, 541, 586, 1039, 2363, 3091
EcoRI GAATTC 1 cut(s) 1778
EcoRII CCWGG 8 cut(s) 29, 127, 603, 1318, 1476, 1766, 2187, 2359
EcoT14I CCWWGG 2 cut(s) 696, 3057
EcoT22I ATGCAT 1 cut(s) 931
EcoT38I GRGCYC 2 cut(s) 103, 3052
ErhI CCWWGG 2 cut(s) 696, 3057
Esp3I CGTCTC 1 cut(s) 613
FalI AAGNNNNNCTT 4 cut(s) 864, 896, 2627, 2659
FaqI GGGAC 2 cut(s) 1025, 1768
FauI CCCGC 1 cut(s) 1545
FauNDI CATATG 1 cut(s) 961
FriOI GRGCYC 2 cut(s) 103, 3052
FspBI CTAG 5 cut(s) 47, 1035, 1112, 1394, 2496
GlaI GCGC 2 cut(s) 247, 1590
GsaI CCCAGC 1 cut(s) 2529
GsuI CTGGAG 7 cut(s) 233, 408, 637, 2262, 2678, 2775, 3204
HaeII RGCGCY 1 cut(s) 1592
HapII CCGG 7 cut(s) 123, 1205, 1659, 2681, 2826, 3186, 3228
HgaI GACGC 1 cut(s) 2684
HhaI GCGC 2 cut(s) 248, 1591
Hin6I GCGC 2 cut(s) 246, 1589
HinP1I GCGC 2 cut(s) 246, 1589
HincII GTYRAC 4 cut(s) 558, 1149, 1413, 2805
HindII GTYRAC 4 cut(s) 558, 1149, 1413, 2805
HindIII AAGCTT 3 cut(s) 311, 1505, 2996
HpaII CCGG 7 cut(s) 123, 1205, 1659, 2681, 2826, 3186, 3228
HphI GGTGA 3 cut(s) 794, 1583, 3092
Hpy99I CGWCG 1 cut(s) 662
HpyCH4IV ACGT 4 cut(s) 622, 1009, 2575, 2613
HpyF10VI GCNNNNNNNGC 9 cut(s) 21, 1253, 1523, 1659, 2134, 2267, 2288, 3004, 3047
HpyF3I CTNAG 9 cut(s) 896, 903, 1545, 1644, 1783, 1973, 2145, 2427, 3051
HpySE526I ACGT 4 cut(s) 622, 1009, 2575, 2613
HspAI GCGC 2 cut(s) 246, 1589
KflI GGGWCCC 1 cut(s) 1039
Kpn2I TCCGGA 2 cut(s) 1204, 2680
LguI GCTCTTC 1 cut(s) 1143
LmnI GCTCC 3 cut(s) 1500, 2683, 2748
LweI GCATC 9 cut(s) 190, 467, 1770, 2073, 2108, 2203, 2227, 2248, 3160
MaeI CTAG 5 cut(s) 47, 1035, 1112, 1394, 2496
MaeII ACGT 4 cut(s) 622, 1009, 2575, 2613
MaeIII GTNAC 7 cut(s) 521, 611, 722, 1076, 1180, 1928, 2794
MfeI CAATTG 3 cut(s) 2193, 3036, 3042
MflI RGATCY 4 cut(s) 715, 1357, 2035, 2313
MhlI GDGCHC 4 cut(s) 103, 2130, 2850, 3052
MlyI GAGTC 3 cut(s) 1750, 1799, 2714
MmeI TCCRAC 3 cut(s) 540, 840, 1977
Mph1103I ATGCAT 1 cut(s) 931
MroI TCCGGA 2 cut(s) 1204, 2680
MroXI GAANNNNTTC 3 cut(s) 789, 875, 1427
MseI TTAA 9 cut(s) 1461, 1485, 1884, 2054, 2831, 2892, 2898, 3113, 3117
MslI CAYNNNNRTG 3 cut(s) 818, 2732, 2853
MspA1I CMGCKG 1 cut(s) 2210
MspI CCGG 7 cut(s) 123, 1205, 1659, 2681, 2826, 3186, 3228
MunI CAATTG 3 cut(s) 2193, 3036, 3042
MvaI CCWGG 8 cut(s) 31, 129, 605, 1320, 1478, 1768, 2189, 2361
MwoI GCNNNNNNNGC 9 cut(s) 21, 1253, 1523, 1659, 2134, 2267, 2288, 3004, 3047
NciI CCSGG 2 cut(s) 123, 3228
NcoI CCATGG 1 cut(s) 696
NdeI CATATG 1 cut(s) 961
NheI GCTAGC 1 cut(s) 1393
NlaIV GGNNCC 7 cut(s) 101, 543, 834, 1040, 1041, 1867, 2365
NmuCI GTSAC 5 cut(s) 521, 611, 1180, 1928, 2794
NsiI ATGCAT 1 cut(s) 931
NspI RCATGY 4 cut(s) 194, 289, 1861, 2452
PacI TTAATTAA 1 cut(s) 3117
PaeI GCATGC 1 cut(s) 289
PagI TCATGA 2 cut(s) 766, 3012
PasI CCCWGGG 2 cut(s) 128, 1767
PceI AGGCCT 1 cut(s) 3056
PciI ACATGT 2 cut(s) 1857, 2448
PciSI GCTCTTC 1 cut(s) 1143
PcsI WCGNNNNNNNCGW 1 cut(s) 2610
PdmI GAANNNNTTC 3 cut(s) 789, 875, 1427
PfeI GAWTC 8 cut(s) 325, 1120, 1199, 1428, 1720, 1802, 2536, 3214
PflFI GACNNNGTC 1 cut(s) 2387
PflMI CCANNNNNTGG 1 cut(s) 1325
PfoI TCCNGGA 2 cut(s) 1318, 1476
PleI GAGTC 3 cut(s) 1750, 1798, 2714
PpsI GAGTC 3 cut(s) 1750, 1798, 2714
Ppu21I YACGTR 2 cut(s) 1010, 2614
PpuMI RGGWCCY 5 cut(s) 541, 586, 1039, 2363, 3091
PscI ACATGT 2 cut(s) 1857, 2448
PsiI TTATAA 1 cut(s) 549
Psp5II RGGWCCY 5 cut(s) 541, 586, 1039, 2363, 3091
Psp6I CCWGG 8 cut(s) 29, 127, 603, 1318, 1476, 1766, 2187, 2359
PspFI CCCAGC 1 cut(s) 2525
PspGI CCWGG 8 cut(s) 29, 127, 603, 1318, 1476, 1766, 2187, 2359
PspN4I GGNNCC 7 cut(s) 101, 543, 834, 1040, 1041, 1867, 2365
PspOMI GGGCCC 1 cut(s) 99
PspPPI RGGWCCY 5 cut(s) 541, 586, 1039, 2363, 3091
PstI CTGCAG 2 cut(s) 226, 889
PsuI RGATCY 4 cut(s) 715, 1357, 2035, 2313
PsyI GACNNNGTC 1 cut(s) 2387
PvuII CAGCTG 1 cut(s) 2210
RseI CAYNNNNRTG 3 cut(s) 818, 2732, 2853
SapI GCTCTTC 1 cut(s) 1143
SaqAI TTAA 9 cut(s) 1461, 1485, 1884, 2054, 2831, 2892, 2898, 3113, 3117
ScaI AGTACT 1 cut(s) 1031
SchI GAGTC 3 cut(s) 1750, 1799, 2714
SduI GDGCHC 4 cut(s) 103, 2130, 2850, 3052
SfaNI GCATC 9 cut(s) 190, 467, 1770, 2073, 2108, 2203, 2227, 2248, 3160
SfcI CTRYAG 3 cut(s) 222, 636, 885
SinI GGWCC 7 cut(s) 541, 586, 774, 1039, 2363, 2683, 3091
SmiMI CAYNNNNRTG 3 cut(s) 818, 2732, 2853
SmlI CTYRAG 3 cut(s) 590, 1361, 2465
SmoI CTYRAG 3 cut(s) 590, 1361, 2465
SnaBI TACGTA 1 cut(s) 2614
SphI GCATGC 1 cut(s) 289
SseBI AGGCCT 1 cut(s) 3056
SsiI CCGC 7 cut(s) 5, 15, 119, 471, 1340, 1552, 2299
SspI AATATT 4 cut(s) 2000, 2410, 2711, 3150
SspMI CTAG 5 cut(s) 47, 1035, 1112, 1394, 2496
StuI AGGCCT 1 cut(s) 3056
StyI CCWWGG 2 cut(s) 696, 3057
TaiI ACGT 4 cut(s) 625, 1012, 2578, 2616
TaqI TCGA 6 cut(s) 67, 206, 235, 657, 1311, 2972
TatI WGTACW 2 cut(s) 278, 1029
TauI GCSGC 4 cut(s) 18, 121, 474, 2302
TfiI GAWTC 8 cut(s) 325, 1120, 1199, 1428, 1720, 1802, 2536, 3214
Tru1I TTAA 9 cut(s) 1461, 1485, 1884, 2054, 2831, 2892, 2898, 3113, 3117
Tru9I TTAA 9 cut(s) 1461, 1485, 1884, 2054, 2831, 2892, 2898, 3113, 3117
TseFI GTSAC 5 cut(s) 521, 611, 1180, 1928, 2794
Tsp45I GTSAC 5 cut(s) 521, 611, 1180, 1928, 2794
TspGWI ACGGA 3 cut(s) 710, 1866, 2619
Tth111I GACNNNGTC 1 cut(s) 2387
Van91I CCANNNNNTGG 1 cut(s) 1325
VpaK11BI GGWCC 7 cut(s) 541, 586, 774, 1039, 2363, 2683, 3091
XagI CCTNNNNNAGG 1 cut(s) 1495
XapI RAATTY 7 cut(s) 533, 1259, 1457, 1666, 1778, 2201, 2263
XceI RCATGY 4 cut(s) 194, 289, 1861, 2452
XmnI GAANNNNTTC 3 cut(s) 789, 875, 1427
XspI CTAG 5 cut(s) 47, 1035, 1112, 1394, 2496
ZrmI AGTACT 1 cut(s) 1031
Zsp2I ATGCAT 1 cut(s) 931
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.