Rw1G034870

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000598
Physical Location & Seq
Reverse (-)
62736795 .. 62742640
5846 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G034870.1

Sequence Viewer

Length: 3333 bp
ATGAGAATAAAGTGGACTACAAAATTCCACTGTGCCAGTTTGATTAAAAGGTACTTTACTAGTAGTGATATGGCGGAGGAAGGCGGGAAGAGCTTTGCCAGGAGAGACCGGCTTTTAGAGATCGAGACAAAGGCTCGAAACTGGTGGGAGGAGAAACAAGTTTTCAGGGCAGAATCTTGTGAAAAGGCTCCCGGACCGGGGGAGAAGTTCTTTGGAAACTTCCCCTTCCCTTATATGAATGGTTTTCTGCATCTCGGACATGCGTTTTCACTCTCGAAGCTGGAGTTTGCTGCGGCCTATCATAGATTGAGAGGCGCAAATGTGCTCCTGCCTTTTGCTTTTCACTGTACTGGCATGCCCATCAAGGCCTCAGCTGATAAGCTTACTAGGGAGATTCAACAGTTTGGAGAACCTCCTGTTTTTCCCGAAAAAGTGGAGAAGGGGAACGAGGAAGTGGAAGTGGAAGCCGAGGATGTCAATGCAGGTGCACCTCCAGCCAAGCATAAGGGAAAGAAGTCGAAGGCTGCATCAAAATCAGGCGGCGAAGTGTACCAATGGGAAATTATGCGAAGCTTTGGCCTCTCTGACAGTGACATATCCAAATTTCAGGATCCTTATAAGTGGTTGACTTATTTTCCCCCATTGGCAGTGGAGGACCTCAAGGCTTTTGGCCTGGGCTGTGACTGGAGACGTTCCTTTATTACTACAGACATGAATCCTTTTTTCGATGCCTTTGTGAGGTGGCAGGTTAGGAAACTGAAATCCATGGGCAAGATTGTCAAAGATCTCCGTTACACGATATACTCTCCGATGGATGGGCAACCGTGTGCAGATCATGATAGGTCCAGTGGTGAAGGAGTTCAGCCTCAAGAATACACTGTCATCAAAATGGAACTGATGGCACCTTTTCCTCCTAAATTGGGAGTGTTGGAGGGGAGAAAAGTGTTCCTTGCTGCAGCAACACTGAGACCTGAGACCATGTATGGACAAACAAATGCATGGGTATTGCCTGATGGTAAGTATGGTGCCTTTGAAATCAATGAAACAGATGTGTTTATTCTTACTCAGAGAGCAGCACGTAATCTTGCTTATCAAAAGTACTCTAGGGTCCCAGAGAAACCTACTTGCTTGGTTGAACTGACTGGTTATGATTTGATTGGCCTTCCATTGAAGTCACCACTAGCACTGAATGAGATTATTTATGCTCTTCCTATGTTGACTGTCCTAACAGACAAAGGTACTGGGATTGTGACCAGTGTACCTACCGATTCTCCGGATGATTATATGGCCTTGCATGACTTGAAATCAAAACCTGCTTTTAGGGCAAAATATGGTGTGAAAGATGAGTGGGTACTGCCCTTTGAGAATATCCCAATCATTGATATTCCAGGATTTGGAAATAATGCTGCGGAAAAGGTCTGTGCAGAACTCAAGATCAAAAGCCAGAATGAGAAAGAGAAGCTAGCAGAAGCCAAGAGGTTGACATACTTGAAAGGATTCAATGAAGGAACAATGATTTTGGGGGAGTTTAAGGGAAGAAAAGTCCAGGAGGTTAAACCCTTGATAAGGAGCAAGCTTATAGAGGCGGGGGAGGCAATATCATATAGTGAACCTGAGAAGCGAGTACTATCACGATCAGGTGATGAATGTGTTGTTGCGCTTACAGATCAATGGTACATCACATACGGGGAAACTGAATGGAAAAAACTGGCTGAAGAGTGCTTGTCCGGCATGAATTTGTATTCTGATGAGACACGGCATGGATTTGAACACACATTGAGCTGGTTGAATCAGTGGGCTTGTTCACGATCTTTTGGTCTTGGGACTCGCATCCCCTGGGATGAAGAATTCTTAGTTGAGTCATTGTCTGATTCAACTATTTACATGGCTTATTACACCATTGCTCACTTCCTACACAATGGAGACATGTATGGTTCCAGCAAATCTGCAATTAAACCTGAACAAATGACTGATGAGGTCTGGGATTATGTTTTCTGTGATGGCCCATATCCTAAATCATCTGAGATCTCATCATCAATTCTGAGTAAGATGAAGCAGGAGTTTGAATATTGGTATCCTTTTGATCTTCGGGTGTCTGGCAAGGATCTAATACAAAATCATTTGACCTTCTGCATCTACAATCATACAGCTATTATGTCCAAGAAGCACTGGCCTCGTGGTTTCAGATGCAATGGGCACATAATGCTCAATGCTCAGAAAATGTCCAAGTCTACAGGGAACTTTAGAACAATTCGCCAGGCAATTGACGAATTTTCTGCTGATGCCACTCGGTTTTCTCTGGCTGATGCTGGAGATGGTGTTGATGATGCAAATTTTGCATTTGACACTGCCAATGCTGCAATCCTGCGGCTCACTAAAGAGATCTCATGGACAGAAGAAGTTTTGGGTGCAGAGTCTTCTTTGAGGCCAGGCCCCCCAACTACTTATGCTGATAGGGTCTTTGCGAATGAGATAAATATTGCTGTGAACAGGACTGAGCAGAATTACAGCGGTTACATGTTCCGAGAAGCCCTCAAGACTGGATTTTATGATCTTCAAGCTGCTAGGGATGAGTATCGGTTTTCATGTGGTGATGGGGGAATGAACCGTGATTTGGTGTGGCGTTTTATGGATGTGCAGACACGTCTTATTACTCCAATCTGCCCACACTATGGAGAATACGTATGGAAGGAACTTTTGAAGAAGAACGGGTTTGTGGTAAACGCAGGATGGCCTTTGGCTGACGCACCGGACCTAACCCTCCAGAGTGCCAATAAGTATTTGCAGGAATCAATCATTTTAATGAGGAAGCTCCTCCAAAAGCAAGTTTTAGGCTCAAAGAAGGGTAATAAGAAGGGTACTCCAGTTACAGTGTTGACAGAAGACCACAATCTAACTGGCTTAATTTATGTGAATGAGCACTTTGATGGATGGAAGGCAGAGTGCCTGAAAATCCTCCAAATTAACTTTAACAGAGACGGTCGTACTTTTTCTTCAGACAAGGTGATGCAAGAAGCATTGCAAAAAAGTTCTATTGGTCAGTCGAAAGATTTTAGACAAATCCAAAAGCTTTGTATGCCTTTCATGAAATTCAAGAAGGATGAGGCAATTGCAATTGGGGCTCAGGCCTTGGATTTGAAGCTGCCTTTTGGCGAGATTGAGGTCCTGCAAGAGAACTTGGACTTAATTAAGAGACAACTTGGTCTTCAAGAGGTAGAAATATTGTCTGCTACAAATCCTGATGCACTTTCTAAAGCCGGTTCTCTTGTCAAACTGTTAGAGCAGAATCCTCCAGCACCCGGAAGCCCTACTGCCATCTTCTTGACCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

1110

Amino Acids

125.67

Weight (kDa)

6.5

Isoelectric Point (pI)

44.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1 PF00133 47 - 128 3.7e-08 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1 PF00133 216 - 778 4.9e-17 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g PF09334 683 - 784 1.3e-08 tRNA synthetases class I (M)
tRNA-synt_1e PF01406 692 - 768 1.2e-08 tRNA synthetases class I (C) catalytic domain
Anticodon_1 PF08264 818 - 947 1.6e-13 Anticodon-binding domain of tRNA ligase
RBD_LARS1 PF24810 972 - 1048 5.9e-06 Leucine--tRNA ligase, RagD-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000598)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09620
fragaria_vesca FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_3g34581 FvH4_4g33720 FvH4_4g33720
malus_domestica MD08G1244600.v1.1 MD15G1439400.v1.1
prunus_persica Prupe.1G582000_v2.0.a1 Prupe.2G015100_v2.0.a1 Prupe.2G123100_v2.0.a1
pyrus_communis pycom08g21210 pycom08g21220 pycom15g38730
rosa_chinensis RchiOBHm_Chr1g0336121 RchiOBHm_Chr1g0353811 RchiOBHm_Chr1g0373331 RchiOBHm_Chr4g0443191 RchiOBHm_Chr4g0443201 RchiOBHm_Chr4g0443211
rosa_laevigata RLG00000003122 RLG00000005948 RLG00000026826 RLG00000028251 RLG00000028252 RLG00000028253 RLG00000029377
rosa_multiflora Rmu_co8002498.1_g000001 Rmu_co8262279.1_g000001 Rmu_co8314139.1_g000001 Rmu_co8393711.1_g000001 Rmu_co8419167.1_g000001 Rmu_sc0007968.1_g000004 Rmu_sc0018919.1_g000004 Rmu_sc0027447.1_g000001
rosa_roxburghii Rroxscaffold_4G00284260 Rroxscaffold_4G00301490 Rroxscaffold_4G00315860 Rroxscaffold_5G00383790 Rroxscaffold_5G00383800
rosa_rugosa Rorug01G0123100 Rorug01G0238700 Rorug01G0377100 Rorug01G0377100 Rorug01G0377200 Rorug01G0377300 Rorug04G0344700 Rorug05G0347900.1
rosa_samantha Rh1AG143800 Rh1AG250300 Rh1AG386300 Rh1BG113400 Rh1BG220800 Rh1BG220900 Rh1BG350400 Rh1CG135500 Rh1CG233100 Rh1CG363500 Rh1DG150200 Rh1DG247700 Rh1DG247800 Rh1DG381300 Rh4AG399400 Rh4AG399500 Rh4BG411000 Rh4BG411100 Rh4BG411200 Rh4CG426500 Rh4CG426600 Rh4DG406100 Rh4DG406200 Rh4DG406300
rosa_wichuraiana Rw0G015190 Rw1G021850 Rw1G034870 Rw4G034440 Rw4G034450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 618
AarI CACCTGC 1 cut(s) 473
AasI GACNNNNNNGTC 1 cut(s) 3201
Acc36I ACCTGC 3 cut(s) 473, 736, 1321
AccB1I GGYRCC 2 cut(s) 901, 1025
AccB7I CCANNNNNTGG 2 cut(s) 1394, 2672
AccI GTMKAC 1 cut(s) 2231
AccIII TCCGGA 1 cut(s) 1273
AciI CCGC 8 cut(s) 74, 84, 293, 540, 1409, 1586, 2368, 2511
AclWI GGATC 3 cut(s) 605, 618, 2112
AcsI RAATTY 7 cut(s) 23, 602, 1735, 1847, 2270, 2332, 3089
AcuI CTGAAG 2 cut(s) 1734, 2979
AflIII ACRYGT 3 cut(s) 1926, 2517, 2642
AhlI ACTAGT 1 cut(s) 59
AjiI CACGTC 1 cut(s) 2645
AjnI CCWGG 7 cut(s) 98, 672, 1387, 1545, 1835, 2256, 2428
AjuI GAANNNNNNNTTGG 2 cut(s) 2597, 2629
Alw21I GWGCWC 3 cut(s) 327, 490, 2922
Alw26I GTCTC 9 cut(s) 99, 119, 682, 961, 968, 1745, 1917, 2970, 3187
Alw44I GTGCAC 1 cut(s) 486
AlwI GGATC 3 cut(s) 605, 618, 2112
Aor13HI TCCGGA 1 cut(s) 1273
ApaLI GTGCAC 1 cut(s) 486
ApeKI GCWGC 9 cut(s) 290, 524, 953, 956, 1073, 1406, 2357, 2561, 3142
ApoI RAATTY 7 cut(s) 23, 602, 1735, 1847, 2270, 2332, 3089
Asp700I GAANNNNTTC 2 cut(s) 858, 1496
AspLEI GCGC 2 cut(s) 317, 1660
AspS9I GGNCC 8 cut(s) 194, 655, 843, 1108, 2003, 2432, 2752, 3163
AsuC2I CCSGG 3 cut(s) 192, 198, 3300
AsuHPI GGTGA 5 cut(s) 863, 1167, 1652, 2603, 3016
AsuNHI GCTAGC 1 cut(s) 1462
AvaII GGWCC 6 cut(s) 194, 655, 843, 1108, 2752, 3163
BaeGI GKGCMC 2 cut(s) 490, 2199
BaeI ACNNNNGTAYC 4 cut(s) 1242, 1275, 2850, 2883
BamHI GGATCC 1 cut(s) 610
BanI GGYRCC 2 cut(s) 901, 1025
BanII GRGCYC 1 cut(s) 3124
BarI GAAGNNNNNNTAC 2 cut(s) 2977, 3009
BauI CACGAG 1 cut(s) 2175
BbsI GAAGAC 3 cut(s) 2409, 2889, 3197
Bbv12I GWGCWC 3 cut(s) 327, 490, 2922
BbvCI CCTCAGC 1 cut(s) 370
BbvI GCAGC 9 cut(s) 277, 511, 940, 968, 1085, 1393, 2344, 2548, 3129
BceAI ACGGC 1 cut(s) 1772
BcgI CGANNNNNNTGC 4 cut(s) 2156, 2190, 2258, 2292
BciT130I CCWGG 7 cut(s) 100, 674, 1389, 1547, 1837, 2258, 2430
BciVI GTATCC 1 cut(s) 2085
BcnI CCSGG 3 cut(s) 192, 198, 3300
BcoDI GTCTC 9 cut(s) 99, 119, 682, 961, 968, 1745, 1917, 2970, 3187
BcuI ACTAGT 1 cut(s) 59
BfaI CTAG 6 cut(s) 60, 387, 1104, 1181, 1463, 2565
BfmI CTRYAG 3 cut(s) 705, 954, 2232
BfuAI ACCTGC 3 cut(s) 473, 736, 1321
BfuI GTATCC 1 cut(s) 2085
BglII AGATCT 3 cut(s) 784, 2025, 2382
BmcAI AGTACT 2 cut(s) 1100, 1626
Bme18I GGWCC 6 cut(s) 194, 655, 843, 1108, 2752, 3163
BmgBI CACGTC 1 cut(s) 2645
BmgT120I GGNCC 8 cut(s) 194, 655, 843, 1108, 2003, 2432, 2752, 3163
BmiI GGNNCC 8 cut(s) 189, 612, 903, 1027, 1109, 1110, 1936, 2434
BmrI ACTGGG 1 cut(s) 1251
BmtI GCTAGC 1 cut(s) 1466
BmuI ACTGGG 1 cut(s) 1251
BpiI GAAGAC 3 cut(s) 2409, 2889, 3197
BplI GAGNNNNNCTC 2 cut(s) 2517, 2549
BpmI CTGGAG 7 cut(s) 302, 477, 706, 2331, 2747, 2847, 3276
Bpu10I CCTNAGC 2 cut(s) 370, 3123
BpuEI CTTGAG 4 cut(s) 644, 852, 1415, 2519
BpuMI CCSGG 3 cut(s) 192, 198, 3300
BsaAI YACGTR 2 cut(s) 1079, 2683
BsaBI GATNNNNATC 1 cut(s) 2574
BsaI GGTCTC 3 cut(s) 99, 961, 968
BsaJI CCNNGG 7 cut(s) 197, 468, 673, 765, 1835, 1836, 3129
BsaWI WCCGGW 2 cut(s) 1273, 2749
BsaXI ACNNNNNCTCC 6 cut(s) 1255, 1285, 2304, 2334, 2667, 2697
Bse118I RCCGGY 2 cut(s) 108, 3257
Bse3DI GCAATG 3 cut(s) 1899, 2197, 3017
Bse8I GATNNNNATC 1 cut(s) 2574
BseAI TCCGGA 1 cut(s) 1273
BseBI CCWGG 7 cut(s) 100, 674, 1389, 1547, 1837, 2258, 2430
BseDI CCNNGG 7 cut(s) 197, 468, 673, 765, 1835, 1836, 3129
BseJI GATNNNNATC 1 cut(s) 2574
BseMI GCAATG 3 cut(s) 1899, 2197, 3017
BseRI GAGGAG 2 cut(s) 164, 2804
BseSI GKGCMC 2 cut(s) 490, 2199
BseXI GCAGC 9 cut(s) 277, 511, 940, 968, 1085, 1393, 2344, 2548, 3129
BsgI GTGCAG 4 cut(s) 849, 1443, 2430, 2657
Bsh1285I CGRYCG 1 cut(s) 2983
BshNI GGYRCC 2 cut(s) 901, 1025
BsiEI CGRYCG 1 cut(s) 2983
BsiHKAI GWGCWC 3 cut(s) 327, 490, 2922
BsiSI CCGG 8 cut(s) 109, 192, 197, 1274, 1728, 2750, 3258, 3300
BslFI GGGAC 2 cut(s) 1094, 1837
BsmAI GTCTC 9 cut(s) 99, 119, 682, 961, 968, 1745, 1917, 2970, 3187
BsmBI CGTCTC 2 cut(s) 682, 2970
BsmFI GGGAC 2 cut(s) 1094, 1837
Bso31I GGTCTC 3 cut(s) 99, 961, 968
Bsp1286I GDGCHC 5 cut(s) 327, 490, 2199, 2922, 3124
Bsp13I TCCGGA 1 cut(s) 1273
Bsp19I CCATGG 1 cut(s) 765
BspACI CCGC 8 cut(s) 74, 84, 293, 540, 1409, 1586, 2368, 2511
BspEI TCCGGA 1 cut(s) 1273
BspHI TCATGA 2 cut(s) 835, 3084
BspLI GGNNCC 8 cut(s) 189, 612, 903, 1027, 1109, 1110, 1936, 2434
BspMAI CTGCAG 1 cut(s) 958
BspMI ACCTGC 3 cut(s) 473, 736, 1321
BspOI GCTAGC 1 cut(s) 1466
BspPI GGATC 3 cut(s) 605, 618, 2112
BspQI GCTCTTC 2 cut(s) 83, 1212
BspT107I GGYRCC 2 cut(s) 901, 1025
BspTNI GGTCTC 3 cut(s) 99, 961, 968
BsrDI GCAATG 3 cut(s) 1899, 2197, 3017
BsrFI RCCGGY 2 cut(s) 108, 3257
BssAI RCCGGY 2 cut(s) 108, 3257
BssECI CCNNGG 7 cut(s) 197, 468, 673, 765, 1835, 1836, 3129
BssSI CACGAG 1 cut(s) 2175
BssT1I CCWWGG 2 cut(s) 765, 3129
Bst2BI CACGAG 1 cut(s) 2175
Bst2UI CCWGG 7 cut(s) 100, 674, 1389, 1547, 1837, 2258, 2430
Bst6I CTCTTC 3 cut(s) 83, 1212, 1710
BstAPI GCANNNNNTGC 2 cut(s) 2203, 2336
BstBAI YACGTR 2 cut(s) 1079, 2683
BstC8I GCNNGC 3 cut(s) 356, 1464, 1574
BstDSI CCRYGG 1 cut(s) 765
BstENI CCTNNNNNAGG 2 cut(s) 736, 1564
BstHHI GCGC 2 cut(s) 317, 1660
BstMAI GTCTC 9 cut(s) 99, 119, 682, 961, 968, 1745, 1917, 2970, 3187
BstMCI CGRYCG 1 cut(s) 2983
BstNI CCWGG 7 cut(s) 100, 674, 1389, 1547, 1837, 2258, 2430
BstNSI RCATGY 4 cut(s) 263, 358, 1930, 2521
BstSFI CTRYAG 3 cut(s) 705, 954, 2232
BstSLI GKGCMC 2 cut(s) 490, 2199
BstSNI TACGTA 1 cut(s) 2683
BstV1I GCAGC 9 cut(s) 277, 511, 940, 968, 1085, 1393, 2344, 2548, 3129
BstV2I GAAGAC 3 cut(s) 2409, 2889, 3197
BstX2I RGATCY 5 cut(s) 610, 784, 2025, 2104, 2382
BstYI RGATCY 5 cut(s) 610, 784, 2025, 2104, 2382
BsuI GTATCC 1 cut(s) 2085
BtgI CCRYGG 1 cut(s) 765
BtrI CACGTC 1 cut(s) 2645
BtsI GCAGTG 2 cut(s) 654, 2346
BveI ACCTGC 3 cut(s) 473, 736, 1321
Cac8I GCNNGC 3 cut(s) 356, 1464, 1574
CciI TCATGA 2 cut(s) 835, 3084
CfoI GCGC 2 cut(s) 317, 1660
Cfr10I RCCGGY 2 cut(s) 108, 3257
Cfr13I GGNCC 8 cut(s) 194, 655, 843, 1108, 2003, 2432, 2752, 3163
CpoI CGGWCCG 1 cut(s) 194
CseI GACGC 1 cut(s) 2753
CspI CGGWCCG 1 cut(s) 194
DrdI GACNNNNNNGTC 1 cut(s) 3201
DseDI GACNNNNNNGTC 1 cut(s) 3201
Eam1104I CTCTTC 3 cut(s) 83, 1212, 1710
EarI CTCTTC 3 cut(s) 83, 1212, 1710
EciI GGCGGA 1 cut(s) 89
Eco105I TACGTA 1 cut(s) 2683
Eco130I CCWWGG 2 cut(s) 765, 3129
Eco147I AGGCCT 2 cut(s) 368, 3128
Eco24I GRGCYC 1 cut(s) 3124
Eco31I GGTCTC 3 cut(s) 99, 961, 968
Eco47I GGWCC 6 cut(s) 194, 655, 843, 1108, 2752, 3163
Eco57I CTGAAG 2 cut(s) 1734, 2979
EcoNI CCTNNNNNAGG 2 cut(s) 736, 1564
EcoO109I RGGNCCY 4 cut(s) 655, 1108, 2432, 3163
EcoRI GAATTC 1 cut(s) 1847
EcoRII CCWGG 7 cut(s) 98, 672, 1387, 1545, 1835, 2256, 2428
EcoT14I CCWWGG 2 cut(s) 765, 3129
EcoT22I ATGCAT 1 cut(s) 1000
EcoT38I GRGCYC 1 cut(s) 3124
ErhI CCWWGG 2 cut(s) 765, 3129
Esp3I CGTCTC 2 cut(s) 682, 2970
FalI AAGNNNNNCTT 2 cut(s) 933, 965
FaqI GGGAC 2 cut(s) 1094, 1837
FauI CCCGC 2 cut(s) 77, 1579
FblI GTMKAC 1 cut(s) 2231
FriOI GRGCYC 1 cut(s) 3124
FspBI CTAG 6 cut(s) 60, 387, 1104, 1181, 1463, 2565
GlaI GCGC 2 cut(s) 316, 1659
GsuI CTGGAG 7 cut(s) 302, 477, 706, 2331, 2747, 2847, 3276
HapII CCGG 8 cut(s) 109, 192, 197, 1274, 1728, 2750, 3258, 3300
HgaI GACGC 1 cut(s) 2753
HhaI GCGC 2 cut(s) 317, 1660
Hin6I GCGC 2 cut(s) 315, 1658
HinP1I GCGC 2 cut(s) 315, 1658
HincII GTYRAC 4 cut(s) 627, 1218, 1482, 2877
HindII GTYRAC 4 cut(s) 627, 1218, 1482, 2877
HindIII AAGCTT 4 cut(s) 380, 571, 1574, 3068
HpaII CCGG 8 cut(s) 109, 192, 197, 1274, 1728, 2750, 3258, 3300
HphI GGTGA 5 cut(s) 863, 1167, 1652, 2603, 3016
HpyCH4IV ACGT 4 cut(s) 691, 1078, 2644, 2682
HpySE526I ACGT 4 cut(s) 691, 1078, 2644, 2682
HspAI GCGC 2 cut(s) 315, 1658
KflI GGGWCCC 1 cut(s) 1108
Kpn2I TCCGGA 1 cut(s) 1273
LguI GCTCTTC 2 cut(s) 83, 1212
LmnI GCTCC 4 cut(s) 193, 330, 1569, 2817
Lsp1109I GCAGC 9 cut(s) 277, 511, 940, 968, 1085, 1393, 2344, 2548, 3129
MaeI CTAG 6 cut(s) 60, 387, 1104, 1181, 1463, 2565
MaeII ACGT 4 cut(s) 691, 1078, 2644, 2682
MaeIII GTNAC 7 cut(s) 590, 680, 791, 1173, 1249, 2513, 2866
MfeI CAATTG 3 cut(s) 2262, 3108, 3114
MflI RGATCY 5 cut(s) 610, 784, 2025, 2104, 2382
MhlI GDGCHC 5 cut(s) 327, 490, 2199, 2922, 3124
MlyI GAGTC 3 cut(s) 1819, 1868, 2423
MmeI TCCRAC 1 cut(s) 909
Mph1103I ATGCAT 1 cut(s) 1000
MroI TCCGGA 1 cut(s) 1273
MroXI GAANNNNTTC 2 cut(s) 858, 1496
MslI CAYNNNNRTG 3 cut(s) 887, 2801, 2925
MspA1I CMGCKG 2 cut(s) 374, 2511
MspI CCGG 8 cut(s) 109, 192, 197, 1274, 1728, 2750, 3258, 3300
MunI CAATTG 3 cut(s) 2262, 3108, 3114
MvaI CCWGG 7 cut(s) 100, 674, 1389, 1547, 1837, 2258, 2430
NciI CCSGG 3 cut(s) 192, 198, 3300
NcoI CCATGG 1 cut(s) 765
NheI GCTAGC 1 cut(s) 1462
NlaIV GGNNCC 8 cut(s) 189, 612, 903, 1027, 1109, 1110, 1936, 2434
NmeAIII GCCGAG 1 cut(s) 493
NmuCI GTSAC 4 cut(s) 590, 680, 1173, 1249
NsiI ATGCAT 1 cut(s) 1000
NspI RCATGY 4 cut(s) 263, 358, 1930, 2521
PacI TTAATTAA 1 cut(s) 3189
PaeI GCATGC 1 cut(s) 358
PagI TCATGA 2 cut(s) 835, 3084
PaqCI CACCTGC 1 cut(s) 473
PasI CCCWGGG 1 cut(s) 1836
PceI AGGCCT 2 cut(s) 368, 3128
PciI ACATGT 2 cut(s) 1926, 2517
PciSI GCTCTTC 2 cut(s) 83, 1212
PdmI GAANNNNTTC 2 cut(s) 858, 1496
PfeI GAWTC 9 cut(s) 173, 394, 715, 1268, 1497, 1789, 1871, 2789, 3286
PflMI CCANNNNNTGG 2 cut(s) 1394, 2672
PfoI TCCNGGA 3 cut(s) 190, 1387, 1545
PleI GAGTC 3 cut(s) 1819, 1867, 2422
PpsI GAGTC 3 cut(s) 1819, 1867, 2422
Ppu21I YACGTR 2 cut(s) 1079, 2683
PpuMI RGGWCCY 3 cut(s) 655, 1108, 3163
PscI ACATGT 2 cut(s) 1926, 2517
PsiI TTATAA 1 cut(s) 618
Psp5II RGGWCCY 3 cut(s) 655, 1108, 3163
Psp6I CCWGG 7 cut(s) 98, 672, 1387, 1545, 1835, 2256, 2428
PspGI CCWGG 7 cut(s) 98, 672, 1387, 1545, 1835, 2256, 2428
PspN4I GGNNCC 8 cut(s) 189, 612, 903, 1027, 1109, 1110, 1936, 2434
PspPI GGNCC 8 cut(s) 194, 655, 843, 1108, 2003, 2432, 2752, 3163
PspPPI RGGWCCY 3 cut(s) 655, 1108, 3163
PstI CTGCAG 1 cut(s) 958
PsuI RGATCY 5 cut(s) 610, 784, 2025, 2104, 2382
PvuII CAGCTG 1 cut(s) 374
RseI CAYNNNNRTG 3 cut(s) 887, 2801, 2925
Rsr2I CGGWCCG 1 cut(s) 194
RsrII CGGWCCG 1 cut(s) 194
SapI GCTCTTC 2 cut(s) 83, 1212
Sau96I GGNCC 8 cut(s) 194, 655, 843, 1108, 2003, 2432, 2752, 3163
ScaI AGTACT 2 cut(s) 1100, 1626
SchI GAGTC 3 cut(s) 1819, 1868, 2423
SduI GDGCHC 5 cut(s) 327, 490, 2199, 2922, 3124
SfcI CTRYAG 3 cut(s) 705, 954, 2232
SinI GGWCC 6 cut(s) 194, 655, 843, 1108, 2752, 3163
SmiMI CAYNNNNRTG 3 cut(s) 887, 2801, 2925
SmlI CTYRAG 4 cut(s) 659, 867, 1430, 2534
SmoI CTYRAG 4 cut(s) 659, 867, 1430, 2534
SnaBI TACGTA 1 cut(s) 2683
SpeI ACTAGT 1 cut(s) 59
SphI GCATGC 1 cut(s) 358
SseBI AGGCCT 2 cut(s) 368, 3128
SsiI CCGC 8 cut(s) 74, 84, 293, 540, 1409, 1586, 2368, 2511
SspI AATATT 3 cut(s) 2069, 2479, 3222
SspMI CTAG 6 cut(s) 60, 387, 1104, 1181, 1463, 2565
StuI AGGCCT 2 cut(s) 368, 3128
StyI CCWWGG 2 cut(s) 765, 3129
TaiI ACGT 4 cut(s) 694, 1081, 2647, 2685
TaqI TCGA 6 cut(s) 123, 136, 275, 518, 726, 3044
TatI WGTACW 3 cut(s) 347, 1098, 1624
TauI GCSGC 3 cut(s) 296, 543, 2371
TfiI GAWTC 9 cut(s) 173, 394, 715, 1268, 1497, 1789, 1871, 2789, 3286
TseFI GTSAC 4 cut(s) 590, 680, 1173, 1249
TseI GCWGC 9 cut(s) 290, 524, 953, 956, 1073, 1406, 2357, 2561, 3142
Tsp45I GTSAC 4 cut(s) 590, 680, 1173, 1249
TspGWI ACGGA 1 cut(s) 779
Van91I CCANNNNNTGG 2 cut(s) 1394, 2672
VneI GTGCAC 1 cut(s) 486
VpaK11BI GGWCC 6 cut(s) 194, 655, 843, 1108, 2752, 3163
XagI CCTNNNNNAGG 2 cut(s) 736, 1564
XapI RAATTY 7 cut(s) 23, 602, 1735, 1847, 2270, 2332, 3089
XceI RCATGY 4 cut(s) 263, 358, 1930, 2521
XcmI CCANNNNNNNNNTGG 1 cut(s) 2894
XmiI GTMKAC 1 cut(s) 2231
XmnI GAANNNNTTC 2 cut(s) 858, 1496
XspI CTAG 6 cut(s) 60, 387, 1104, 1181, 1463, 2565
ZrmI AGTACT 2 cut(s) 1100, 1626
Zsp2I ATGCAT 1 cut(s) 1000
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.