Rw4G034450

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000598
Physical Location & Seq
Reverse (-)
61516910 .. 61522148
5239 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G034450.1

Sequence Viewer

Length: 2481 bp
ATGCCCATCAAAAGTATTGGCCTGTCTGCCATTGATATATCAGAACTCCAGAATCCATCGAACTGGTTGAGCTGCTTCCCTCGAGTTGCAGTGAAAGAGCTCAAGGCATTTGGCTTGGGCTGTGACTGGAGGCGCTCCTTTATTACCACAGACATGAACCCATACTTTGACAAGTTTGTGAGATGGCAGATGAGGAAACTAAAATCTATGGGTAAGATTGTGAAGGATGTCCGTTACACAATCTACTCTCCTTTGGATGGCCAGCCATGTGCAGATCATGATAGGGAAACTGGTGAAGGAGTTCGGCCCCAAGAGTACACCATCATCAAAATGGAGGTGGTGGCACCTTTTCCTCCTAAACTGGGAGTGTTGAAGGGAAAGAAAGTCTTCCTTGCTGCCGCAACATTGAGACCTGAGACTTCTTCTGGTCAAACAAATGCATGGGTGTTGCCTGATGGCAAGTATGGAGCCTTTGAAATCAATGAAACAGATGTCTTTATTCTTACGCAGAGAGCAGCACTTAATCTTGCCTATCAGAAGTACTCTAGGGTTCCAGAAAAGCCTTCTTGCTTGGTTGAGATGACTGGCTATGATTTGATTGGCCTTCCATTGAAGTCTCCACTTGCACTCAATCAGATCGTTTATGCCCTTCCTATGTTGACCGTCCTAACAGACAAAGGTACTGGGATATTGACTAGTGTACCTAGTGATGCTCCTGATGATTATATGGCCTTGGAAGATTTAAAATTGAAACCAGCTTTTAGAGAAAAATATGGTGTGAAGGATGAATGGGTCATGCCCTTTGATATCATTCCAATAATCAAAATTCCGGAATATGGAGACAAGGCGGCTAAAAAGGTTGTGGAAGATCTCAAAATCAAAAGTCAGAATGAGATAGAGAAGCTAGCAGAAGCCAAGAGGTTGACATCCTTGAAAGGTTTAACTGAAGGAAAACTGCTTGTGGGAGAATTCAAAGAAAGGATAGTCCAGGAGGCAAAACCATTGATAAGGAGCAGGCTAATAGAGGCAAGTGATGCAATCATGTATAGCGAGCCTGAAAAGCTGTGGGCTTGTTCTCGATCTTTTGGTCTTGGTACACGCATTCCCTGGGATGAAGAATACTTGGTCGAGCCGTTATCCGATTCAACTCTTTACATGGCTTATTACACCGTTGCTCACCTGTTACACAATGGAGACATGTATGGCAGCTCCAACAGCACATCTGCAGTAAGAGCTGAACAAATGACCGATGAGGTTTGGGATTTTATTTTCTGTGATGGCTCGTATCCACAATCATCTGATATCACACCATCAATTCTTAACAAGATGAAGCAGGAGTTTGAATATTGGTATCCTTTTGATCTTGAGGTGTCTGGTAAGGATTTAATCCAGAATCATACCTTCTGCATTTACAACCATACTGCACTTCTGCGCAAGAAGCACTGGCCTAGTGCGTTTAGATGCAATGGGCACCTGATGCTCAAATCCACGAAGAATGCTAATGGTGCTGGAAATTTTATATCATTGAGACACGCAATTGATCACTATTCTGCTGATGCCACGCGGTTTGCTTTGGCTGATATTAGTGATGGTATTGACGATGCATATTTTTCATTTGAAACTGCAAATGCGGCCATCATACGTCTCACTAAGGAGATAGATTGGATTGATCGGATGAAAGATAAAGATTTGGGTAAAGACTCTTCTCTGAAAATAGGTTCCCCTTGTACCTTCTCTGACCGGGTCTTTCTGAATGAAATAAATATTGCTGTGAAGAGGACTGAGCAAAATTACCAGGGTTGCATGTTCAGGGAAGCCCTAAAGACTGGCTTTTACGATCTTCAAGCTGCCAGGGATTGGTACAGGATTTCATGTGGTGCTACTAATGCAATGAACCACGATTTGGTGTGGCGTTTTATAGATGTGCAGACCCGTCTTATTGCTCCAATTTGTCCCCACTATGCAGAACATGTCTGGAGGGAACTTTTAGAGAGGGAAGGATTTGCAGTAAATGCAGGCTGGCCCGCAGCTGATGCTCCAGATCTAACACTCCAGAGTGCGAATAAGTATTTGCAAGACTCAATTGATTCAATAAGGAAGCAGCATCTATCAACTTCAAAGAAAACCAATATGGGTGCTTCAGCTACTTCTTTGACAGAAGCGTTGATATATGTTAAGGAGAAATATGACGGATGGAAAGCAGAATGCTTAAAAATACTTCAACATGACTTAAATAGAGATACTCTCACTTCTGCTCAAGAGGACAGGGAGATATTTGAGGCATTGAAGGCTAGTTCTTTTGGTCAAGATGATGATTTTAGCCAAAAGAACTGTATGGAATTTTTGAAGTTCAAGAAGGAGAAGGCAGTTGCACTTGGAGTTCAGGCATTGGACTTGAAGCTACCTTTCGGAGAGATTGAAGTCCTTGAACAGAACTTGGACTTGATTAAAAAACAAATACATGGTCTTGAAGAGGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

826

Amino Acids

93.66

Weight (kDa)

5.82

Isoelectric Point (pI)

41.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1g PF09334 444 - 543 6.5e-06 tRNA synthetases class I (M)
Anticodon_1 PF08264 580 - 700 1.9e-16 Anticodon-binding domain of tRNA ligase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000598)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09620
fragaria_vesca FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_3g34581 FvH4_4g33720 FvH4_4g33720
malus_domestica MD08G1244600.v1.1 MD15G1439400.v1.1
prunus_persica Prupe.1G582000_v2.0.a1 Prupe.2G015100_v2.0.a1 Prupe.2G123100_v2.0.a1
pyrus_communis pycom08g21210 pycom08g21220 pycom15g38730
rosa_chinensis RchiOBHm_Chr1g0336121 RchiOBHm_Chr1g0353811 RchiOBHm_Chr1g0373331 RchiOBHm_Chr4g0443191 RchiOBHm_Chr4g0443201 RchiOBHm_Chr4g0443211
rosa_laevigata RLG00000003122 RLG00000005948 RLG00000026826 RLG00000028251 RLG00000028252 RLG00000028253 RLG00000029377
rosa_multiflora Rmu_co8002498.1_g000001 Rmu_co8262279.1_g000001 Rmu_co8314139.1_g000001 Rmu_co8393711.1_g000001 Rmu_co8419167.1_g000001 Rmu_sc0007968.1_g000004 Rmu_sc0018919.1_g000004 Rmu_sc0027447.1_g000001
rosa_roxburghii Rroxscaffold_4G00284260 Rroxscaffold_4G00301490 Rroxscaffold_4G00315860 Rroxscaffold_5G00383790 Rroxscaffold_5G00383800
rosa_rugosa Rorug01G0123100 Rorug01G0238700 Rorug01G0377100 Rorug01G0377100 Rorug01G0377200 Rorug01G0377300 Rorug04G0344700 Rorug05G0347900.1
rosa_samantha Rh1AG143800 Rh1AG250300 Rh1AG386300 Rh1BG113400 Rh1BG220800 Rh1BG220900 Rh1BG350400 Rh1CG135500 Rh1CG233100 Rh1CG363500 Rh1DG150200 Rh1DG247700 Rh1DG247800 Rh1DG381300 Rh4AG399400 Rh4AG399500 Rh4BG411000 Rh4BG411100 Rh4BG411200 Rh4CG426500 Rh4CG426600 Rh4DG406100 Rh4DG406200 Rh4DG406300
rosa_wichuraiana Rw0G015190 Rw1G021850 Rw1G034870 Rw4G034440 Rw4G034450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1433
AccB1I GGYRCC 2 cut(s) 343, 1470
AccB7I CCANNNNNTGG 2 cut(s) 1857, 1903
AccII CGCG 1 cut(s) 1564
AccIII TCCGGA 1 cut(s) 829
AciI CCGC 5 cut(s) 399, 848, 1564, 1631, 2025
AcoI YGGCCR 2 cut(s) 259, 1632
AcsI RAATTY 4 cut(s) 825, 968, 1513, 2339
AcuI CTGAAG 2 cut(s) 966, 2124
AfaI GTAC 7 cut(s) 317, 542, 682, 702, 1096, 1729, 1862
AfiI CCNNNNNNNGG 5 cut(s) 257, 362, 836, 1857, 1903
AflIII ACRYGT 2 cut(s) 1197, 1969
AhlI ACTAGT 1 cut(s) 695
AjnI CCWGG 4 cut(s) 987, 1106, 1794, 1850
AjuI GAANNNNNNNTTGG 2 cut(s) 1886, 1918
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 7 cut(s) 403, 410, 621, 834, 1188, 1522, 1649
Ama87I CYCGRG 1 cut(s) 81
Aor13HI TCCGGA 1 cut(s) 829
AoxI GGCC 8 cut(s) 19, 259, 305, 601, 729, 1445, 1632, 2021
ApeKI GCWGC 7 cut(s) 72, 395, 515, 1206, 1847, 2027, 2101
ApoI RAATTY 4 cut(s) 825, 968, 1513, 2339
ArsI GACNNNNNNTTYG 2 cut(s) 2449, 2481
Asp700I GAANNNNTTC 2 cut(s) 300, 386
AspLEI GCGC 2 cut(s) 135, 1434
AspS9I GGNCC 2 cut(s) 306, 2022
AsuC2I CCSGG 1 cut(s) 1742
AsuHPI GGTGA 2 cut(s) 305, 1169
AsuNHI GCTAGC 1 cut(s) 904
AvaI CYCGRG 1 cut(s) 81
BaeGI GKGCMC 1 cut(s) 1473
BaeI ACNNNNGTAYC 2 cut(s) 684, 717
BalI TGGCCA 1 cut(s) 261
BanI GGYRCC 2 cut(s) 343, 1470
BanII GRGCYC 1 cut(s) 102
BarI GAAGNNNNNNTAC 2 cut(s) 1687, 1719
BbsI GAAGAC 1 cut(s) 379
Bbv12I GWGCWC 1 cut(s) 102
BbvI GCAGC 7 cut(s) 59, 382, 527, 1218, 1834, 2039, 2113
BceAI ACGGC 1 cut(s) 1117
BciT130I CCWGG 4 cut(s) 989, 1108, 1796, 1852
BciVI GTATCC 2 cut(s) 1296, 1362
BclI TGATCA 1 cut(s) 1540
BcnI CCSGG 1 cut(s) 1742
BcoDI GTCTC 7 cut(s) 403, 410, 621, 834, 1188, 1522, 1649
BcuI ACTAGT 1 cut(s) 695
BfaI CTAG 6 cut(s) 546, 696, 705, 905, 1449, 2292
BfmI CTRYAG 1 cut(s) 1224
BfoI RGCGCY 1 cut(s) 136
BfuI GTATCC 2 cut(s) 1296, 1362
BglII AGATCT 2 cut(s) 868, 2041
BmcAI AGTACT 1 cut(s) 542
Bme1390I CCNGG 5 cut(s) 989, 1108, 1742, 1796, 1852
BmeT110I CYCGRG 1 cut(s) 81
BmgT120I GGNCC 2 cut(s) 306, 2022
BmiI GGNNCC 6 cut(s) 308, 345, 469, 552, 1472, 1720
BmrFI CCNGG 5 cut(s) 989, 1108, 1742, 1796, 1852
BmrI ACTGGG 2 cut(s) 371, 693
BmsI GCATC 8 cut(s) 700, 1024, 1451, 1467, 1546, 1591, 2023, 2113
BmtI GCTAGC 1 cut(s) 908
BmuI ACTGGG 2 cut(s) 371, 693
BpiI GAAGAC 1 cut(s) 379
BplI GAGNNNNNCTC 2 cut(s) 2229, 2261
BpmI CTGGAG 5 cut(s) 32, 148, 1996, 2022, 2036
BpuEI CTTGAG 3 cut(s) 86, 1385, 2241
BpuMI CCSGG 1 cut(s) 1742
BsaI GGTCTC 1 cut(s) 403
BsaJI CCNNGG 5 cut(s) 732, 1106, 1107, 1795, 1851
BsaWI WCCGGW 1 cut(s) 829
Bsc4I CCNNNNNNNGG 5 cut(s) 257, 362, 836, 1857, 1903
Bse1I ACTGG 8 cut(s) 68, 131, 295, 366, 589, 688, 1448, 1831
Bse3DI GCAATG 2 cut(s) 1471, 1896
BseAI TCCGGA 1 cut(s) 829
BseBI CCWGG 4 cut(s) 989, 1108, 1796, 1852
BseDI CCNNGG 5 cut(s) 732, 1106, 1107, 1795, 1851
BseGI GGATG 7 cut(s) 232, 262, 790, 926, 1117, 1680, 2198
BseLI CCNNNNNNNGG 5 cut(s) 257, 362, 836, 1857, 1903
BseMI GCAATG 2 cut(s) 1471, 1896
BseMII CTCAG 2 cut(s) 405, 1773
BseNI ACTGG 8 cut(s) 68, 131, 295, 366, 589, 688, 1448, 1831
BseSI GKGCMC 1 cut(s) 1473
BseXI GCAGC 7 cut(s) 59, 382, 527, 1218, 1834, 2039, 2113
BsgI GTGCAG 3 cut(s) 291, 1407, 1946
Bsh1236I CGCG 1 cut(s) 1564
BshFI GGCC 8 cut(s) 21, 261, 307, 603, 731, 1447, 1634, 2023
BshNI GGYRCC 2 cut(s) 343, 1470
BsiHKAI GWGCWC 1 cut(s) 102
BsiHKCI CYCGRG 1 cut(s) 81
BsiSI CCGG 2 cut(s) 830, 1741
BslFI GGGAC 1 cut(s) 1938
BslI CCNNNNNNNGG 5 cut(s) 257, 362, 836, 1857, 1903
BsmAI GTCTC 7 cut(s) 403, 410, 621, 834, 1188, 1522, 1649
BsmBI CGTCTC 1 cut(s) 1649
BsmFI GGGAC 1 cut(s) 1938
BsmI GAATGC 3 cut(s) 1101, 1501, 2210
BsnI GGCC 8 cut(s) 21, 261, 307, 603, 731, 1447, 1634, 2023
Bso31I GGTCTC 1 cut(s) 403
BsoBI CYCGRG 1 cut(s) 81
Bsp1286I GDGCHC 2 cut(s) 102, 1473
Bsp13I TCCGGA 1 cut(s) 829
Bsp143I GATC 9 cut(s) 274, 636, 868, 1079, 1360, 1540, 1669, 1837, 2041
BspACI CCGC 5 cut(s) 399, 848, 1564, 1631, 2025
BspANI GGCC 8 cut(s) 21, 261, 307, 603, 731, 1447, 1634, 2023
BspCNI CTCAG 2 cut(s) 406, 1774
BspEI TCCGGA 1 cut(s) 829
BspFNI CGCG 1 cut(s) 1564
BspHI TCATGA 1 cut(s) 277
BspLI GGNNCC 6 cut(s) 308, 345, 469, 552, 1472, 1720
BspMAI CTGCAG 1 cut(s) 1228
BspOI GCTAGC 1 cut(s) 908
BspT107I GGYRCC 2 cut(s) 343, 1470
BspTNI GGTCTC 1 cut(s) 403
BsrDI GCAATG 2 cut(s) 1471, 1896
BsrI ACTGG 8 cut(s) 68, 131, 295, 366, 589, 688, 1448, 1831
BssECI CCNNGG 5 cut(s) 732, 1106, 1107, 1795, 1851
BssMI GATC 9 cut(s) 274, 636, 868, 1079, 1360, 1540, 1669, 1837, 2041
BssT1I CCWWGG 1 cut(s) 732
Bst2UI CCWGG 4 cut(s) 989, 1108, 1796, 1852
Bst4CI ACNGT 3 cut(s) 664, 1171, 2333
Bst6I CTCTTC 3 cut(s) 1708, 1769, 2466
BstAPI GCANNNNNTGC 4 cut(s) 1034, 1477, 2012, 2033
BstC8I GCNNGC 7 cut(s) 263, 906, 1016, 1052, 2017, 2021, 2025
BstDEI CTNAG 3 cut(s) 414, 1650, 1782
BstF5I GGATG 7 cut(s) 232, 262, 790, 926, 1117, 1680, 2198
BstFNI CGCG 1 cut(s) 1564
BstH2I RGCGCY 1 cut(s) 136
BstHHI GCGC 2 cut(s) 135, 1434
BstKTI GATC 9 cut(s) 277, 639, 871, 1082, 1363, 1543, 1672, 1840, 2044
BstMAI GTCTC 7 cut(s) 403, 410, 621, 834, 1188, 1522, 1649
BstMBI GATC 9 cut(s) 274, 636, 868, 1079, 1360, 1540, 1669, 1837, 2041
BstNI CCWGG 4 cut(s) 989, 1108, 1796, 1852
BstNSI RCATGY 3 cut(s) 1201, 1807, 1973
BstSCI CCNGG 5 cut(s) 987, 1106, 1740, 1794, 1850
BstSFI CTRYAG 1 cut(s) 1224
BstSLI GKGCMC 1 cut(s) 1473
BstUI CGCG 1 cut(s) 1564
BstV1I GCAGC 7 cut(s) 59, 382, 527, 1218, 1834, 2039, 2113
BstV2I GAAGAC 1 cut(s) 379
BstX2I RGATCY 2 cut(s) 868, 2041
BstXI CCANNNNNNTGG 1 cut(s) 63
BstYI RGATCY 2 cut(s) 868, 2041
BsuI GTATCC 2 cut(s) 1296, 1362
BsuRI GGCC 8 cut(s) 21, 261, 307, 603, 731, 1447, 1634, 2023
BtsCI GGATG 7 cut(s) 232, 262, 790, 926, 1117, 1680, 2198
BtsI GCAGTG 1 cut(s) 96
BtsIMutI CAGTG 2 cut(s) 96, 1441
Cac8I GCNNGC 7 cut(s) 263, 906, 1016, 1052, 2017, 2021, 2025
CciI TCATGA 1 cut(s) 277
CfoI GCGC 2 cut(s) 135, 1434
Cfr13I GGNCC 2 cut(s) 306, 2022
Csp6I GTAC 7 cut(s) 316, 541, 681, 701, 1095, 1728, 1861
CspCI CAANNNNNGTGG 2 cut(s) 1549, 1584
CviQI GTAC 7 cut(s) 316, 541, 681, 701, 1095, 1728, 1861
DdeI CTNAG 3 cut(s) 414, 1650, 1782
DpnI GATC 9 cut(s) 276, 638, 870, 1081, 1362, 1542, 1671, 1839, 2043
DpnII GATC 9 cut(s) 274, 636, 868, 1079, 1360, 1540, 1669, 1837, 2041
DraI TTTAAA 1 cut(s) 744
EaeI YGGCCR 2 cut(s) 259, 1632
Eam1104I CTCTTC 3 cut(s) 1708, 1769, 2466
EarI CTCTTC 3 cut(s) 1708, 1769, 2466
Ecl136II GAGCTC 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 732
Eco24I GRGCYC 1 cut(s) 102
Eco31I GGTCTC 1 cut(s) 403
Eco32I GATATC 2 cut(s) 808, 1303
Eco53kI GAGCTC 1 cut(s) 100
Eco57I CTGAAG 2 cut(s) 966, 2124
Eco88I CYCGRG 1 cut(s) 81
EcoICRI GAGCTC 1 cut(s) 100
EcoRI GAATTC 1 cut(s) 968
EcoRII CCWGG 4 cut(s) 987, 1106, 1794, 1850
EcoRV GATATC 2 cut(s) 808, 1303
EcoT14I CCWWGG 1 cut(s) 732
EcoT22I ATGCAT 2 cut(s) 442, 1606
EcoT38I GRGCYC 1 cut(s) 102
ErhI CCWWGG 1 cut(s) 732
Esp3I CGTCTC 1 cut(s) 1649
FalI AAGNNNNNCTT 6 cut(s) 371, 403, 375, 407, 1814, 1846
FaqI GGGAC 1 cut(s) 1938
FauI CCCGC 1 cut(s) 2032
FbaI TGATCA 1 cut(s) 1540
FokI GGATG 7 cut(s) 239, 269, 797, 913, 1124, 1687, 2205
FriOI GRGCYC 1 cut(s) 102
FspBI CTAG 6 cut(s) 546, 696, 705, 905, 1449, 2292
FspI TGCGCA 1 cut(s) 1433
GlaI GCGC 2 cut(s) 134, 1433
GsuI CTGGAG 5 cut(s) 32, 148, 1996, 2022, 2036
HaeII RGCGCY 1 cut(s) 136
HaeIII GGCC 8 cut(s) 21, 261, 307, 603, 731, 1447, 1634, 2023
HapII CCGG 2 cut(s) 830, 1741
HhaI GCGC 2 cut(s) 135, 1434
Hin6I GCGC 2 cut(s) 133, 1432
HinP1I GCGC 2 cut(s) 133, 1432
HincII GTYRAC 2 cut(s) 660, 924
HindII GTYRAC 2 cut(s) 660, 924
HinfI GANTC 6 cut(s) 52, 1142, 1393, 1700, 2078, 2087
HpaII CCGG 2 cut(s) 830, 1741
HphI GGTGA 2 cut(s) 305, 1169
Hpy166II GTNNAC 5 cut(s) 318, 660, 701, 924, 1097
Hpy8I GTNNAC 5 cut(s) 318, 660, 701, 924, 1097
HpyCH4III ACNGT 3 cut(s) 664, 1171, 2333
HpyCH4IV ACGT 1 cut(s) 1642
HpyF3I CTNAG 3 cut(s) 414, 1650, 1782
HpySE526I ACGT 1 cut(s) 1642
HspAI GCGC 2 cut(s) 133, 1432
Kpn2I TCCGGA 1 cut(s) 829
Ksp22I TGATCA 1 cut(s) 1540
Kzo9I GATC 9 cut(s) 274, 636, 868, 1079, 1360, 1540, 1669, 1837, 2041
LmnI GCTCC 7 cut(s) 140, 467, 718, 1011, 1214, 1948, 2041
Lsp1109I GCAGC 7 cut(s) 59, 382, 527, 1218, 1834, 2039, 2113
LweI GCATC 8 cut(s) 700, 1024, 1451, 1467, 1546, 1591, 2023, 2113
MaeI CTAG 6 cut(s) 546, 696, 705, 905, 1449, 2292
MaeII ACGT 1 cut(s) 1642
MaeIII GTNAC 3 cut(s) 122, 233, 1182
MalI GATC 9 cut(s) 276, 638, 870, 1081, 1362, 1542, 1671, 1839, 2043
MboI GATC 9 cut(s) 274, 636, 868, 1079, 1360, 1540, 1669, 1837, 2041
MboII GAAGA 9 cut(s) 379, 414, 749, 878, 1127, 1504, 1695, 1786, 1832
MfeI CAATTG 2 cut(s) 1536, 2082
MflI RGATCY 2 cut(s) 868, 2041
MhlI GDGCHC 2 cut(s) 102, 1473
MlsI TGGCCA 1 cut(s) 261
MluNI TGGCCA 1 cut(s) 261
MlyI GAGTC 2 cut(s) 1694, 2072
MmeI TCCRAC 1 cut(s) 1236
Mox20I TGGCCA 1 cut(s) 261
Mph1103I ATGCAT 2 cut(s) 442, 1606
MroI TCCGGA 1 cut(s) 829
MroXI GAANNNNTTC 2 cut(s) 300, 386
MscI TGGCCA 1 cut(s) 261
MseI TTAA 9 cut(s) 522, 743, 941, 1320, 1385, 2175, 2210, 2231, 2448
MslI CAYNNNNRTG 2 cut(s) 152, 329
Msp20I TGGCCA 1 cut(s) 261
MspA1I CMGCKG 1 cut(s) 2030
MspI CCGG 2 cut(s) 830, 1741
MspR9I CCNGG 5 cut(s) 989, 1108, 1742, 1796, 1852
MunI CAATTG 2 cut(s) 1536, 2082
Mva1269I GAATGC 3 cut(s) 1101, 1501, 2210
MvaI CCWGG 4 cut(s) 989, 1108, 1796, 1852
MvnI CGCG 1 cut(s) 1564
NciI CCSGG 1 cut(s) 1742
NdeII GATC 9 cut(s) 274, 636, 868, 1079, 1360, 1540, 1669, 1837, 2041
NheI GCTAGC 1 cut(s) 904
NlaIV GGNNCC 6 cut(s) 308, 345, 469, 552, 1472, 1720
NmuCI GTSAC 1 cut(s) 122
NsbI TGCGCA 1 cut(s) 1433
NsiI ATGCAT 2 cut(s) 442, 1606
NspI RCATGY 3 cut(s) 1201, 1807, 1973
PaeR7I CTCGAG 1 cut(s) 81
PagI TCATGA 1 cut(s) 277
PasI CCCWGGG 1 cut(s) 1107
PciI ACATGT 2 cut(s) 1197, 1969
PctI GAATGC 3 cut(s) 1101, 1501, 2210
PdmI GAANNNNTTC 2 cut(s) 300, 386
PfeI GAWTC 4 cut(s) 52, 1142, 1393, 2087
PflFI GACNNNGTC 1 cut(s) 1742
PflMI CCANNNNNTGG 2 cut(s) 1857, 1903
PfoI TCCNGGA 1 cut(s) 987
PleI GAGTC 2 cut(s) 1694, 2072
PpsI GAGTC 2 cut(s) 1694, 2072
PscI ACATGT 2 cut(s) 1197, 1969
Psp124BI GAGCTC 1 cut(s) 102
Psp6I CCWGG 4 cut(s) 987, 1106, 1794, 1850
PspGI CCWGG 4 cut(s) 987, 1106, 1794, 1850
PspN4I GGNNCC 6 cut(s) 308, 345, 469, 552, 1472, 1720
PspPI GGNCC 2 cut(s) 306, 2022
PspXI VCTCGAGB 1 cut(s) 81
PstI CTGCAG 1 cut(s) 1228
PsuI RGATCY 2 cut(s) 868, 2041
PsyI GACNNNGTC 1 cut(s) 1742
PvuII CAGCTG 1 cut(s) 2030
RsaI GTAC 7 cut(s) 317, 542, 682, 702, 1096, 1729, 1862
RsaNI GTAC 7 cut(s) 316, 541, 681, 701, 1095, 1728, 1861
RseI CAYNNNNRTG 2 cut(s) 152, 329
SacI GAGCTC 1 cut(s) 102
SaqAI TTAA 9 cut(s) 522, 743, 941, 1320, 1385, 2175, 2210, 2231, 2448
Sau3AI GATC 9 cut(s) 274, 636, 868, 1079, 1360, 1540, 1669, 1837, 2041
Sau96I GGNCC 2 cut(s) 306, 2022
ScaI AGTACT 1 cut(s) 542
SchI GAGTC 2 cut(s) 1694, 2072
ScrFI CCNGG 5 cut(s) 989, 1108, 1742, 1796, 1852
SduI GDGCHC 2 cut(s) 102, 1473
SfaNI GCATC 8 cut(s) 700, 1024, 1451, 1467, 1546, 1591, 2023, 2113
SfcI CTRYAG 1 cut(s) 1224
Sfr274I CTCGAG 1 cut(s) 81
SlaI CTCGAG 1 cut(s) 81
SmiMI CAYNNNNRTG 2 cut(s) 152, 329
SmlI CTYRAG 4 cut(s) 81, 101, 1364, 2256
SmoI CTYRAG 4 cut(s) 81, 101, 1364, 2256
SpeI ACTAGT 1 cut(s) 695
SsiI CCGC 5 cut(s) 399, 848, 1564, 1631, 2025
SspI AATATT 2 cut(s) 1346, 1765
SspMI CTAG 6 cut(s) 546, 696, 705, 905, 1449, 2292
SstI GAGCTC 1 cut(s) 102
StyD4I CCNGG 5 cut(s) 987, 1106, 1740, 1794, 1850
StyI CCWWGG 1 cut(s) 732
TaaI ACNGT 3 cut(s) 664, 1171, 2333
TaiI ACGT 1 cut(s) 1645
TaqI TCGA 4 cut(s) 59, 82, 1078, 1128
TaqII GACCGA 1 cut(s) 1262
TatI WGTACW 2 cut(s) 315, 540
TauI GCSGC 3 cut(s) 401, 851, 1634
TfiI GAWTC 4 cut(s) 52, 1142, 1393, 2087
Tru1I TTAA 9 cut(s) 522, 743, 941, 1320, 1385, 2175, 2210, 2231, 2448
Tru9I TTAA 9 cut(s) 522, 743, 941, 1320, 1385, 2175, 2210, 2231, 2448
TscAI CASTG 2 cut(s) 96, 1448
TseFI GTSAC 1 cut(s) 122
TseI GCWGC 7 cut(s) 72, 395, 515, 1206, 1847, 2027, 2101
Tsp45I GTSAC 1 cut(s) 122
TspGWI ACGGA 2 cut(s) 221, 2205
TspRI CASTG 2 cut(s) 96, 1448
Tth111I GACNNNGTC 1 cut(s) 1742
Van91I CCANNNNNTGG 2 cut(s) 1857, 1903
XapI RAATTY 4 cut(s) 825, 968, 1513, 2339
XceI RCATGY 3 cut(s) 1201, 1807, 1973
XcmI CCANNNNNNNNNTGG 1 cut(s) 328
XhoI CTCGAG 1 cut(s) 81
XmnI GAANNNNTTC 2 cut(s) 300, 386
XspI CTAG 6 cut(s) 546, 696, 705, 905, 1449, 2292
ZrmI AGTACT 1 cut(s) 542
Zsp2I ATGCAT 2 cut(s) 442, 1606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.