Rroxscaffold_4G00284260

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000598
Physical Location & Seq
Reverse (-)
5895749 .. 5900946
5198 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00284260.1

Sequence Viewer

Length: 3330 bp
ATGAGAATAAAGTGGACTACAAAATTCCACTGTGCCAGTTTGATTAAAAGGTACTTTACTAGTAGTGATATGGCGGAGGAAGGCGGGAAGAGCTTTGCCAGGAGAGACCGGCTTTTAGAGATCGAGGCAAAGGCTCGAAACTGGTGGGAGGAGAAGCAAGTTTTCAAGGCAGAGTCTTGTGAGAAGCCTCCTGGACCGGGGGAGAAGTTCTTTGGAAACTTCCCCTTCCCTTATATGAATGGTTTTCTGCATCTCGGACATGCTTTTTCACTCTCAAAGCTGGAGTTTGCTGCGGCCTATCATAGATTGAGAGGCGCAAATGTGCTCCTGCCTTTTGCTTTTCACTGTACTGGCATGCCCATCAAGGCCTCCGCCGATAAGCTTACTAGGGAGATTCAACAGTTTGGAGAACCTCCTGTTTTTCCCAAAAAGGAGGAGAAGGGGAATGAGGAAGTGGAAGTGGAAGCCGAGGATGTCAATGCAGGTGCACCTCCAGCCAAGCATAAGGGAAAGAAGTCGAAGGCTGCATCAAAATCAACCGGCGAAGTGTACCAATGGGAAATTATGCGAAGCTTTGGCCTCTCCGACAGTGACATATCCAAATTTCAGGATCCTTATAAGTGGTTGACTTATTTTCCCCCATTGGCAGTGGAGGACCTCAAGGCTTTTGGCCTGGGCTGTGACTGGAGACGTTCCTTTATTACTACAGACATGAATCCTTTTTTCGACGCCTTTGTGAGGTGGCAGGTTAGGAAACTGAAATCCATGGGTAAGATTGTCAAAGATCTCCGTTACACGATATACTCTCCTATGGATGGGCAACCGTGTGCAGATCATGATAGGTCCAGTGGTGAAGGAGTTCAGCCTCAAGAATACACCGTCATCAAAATGGAACTGATGGCACCTTTTCCTCCTAAATTGGGAGTGTTGGAGGGGAGAAAAGTGTTCCTTGCTGCAGCAACACTGAGACCTGAGACCATGTATGGACAAACAAATGCATGGGTATTGCCTGATGGTAAGTATGGTGCCTTTGAAATCAATGAAACAGATGTGTATATTCTTACTCAGAGAGCAGCACGTAATCTTGCTTATCAAAAGTACTCTAGGGTCCCAGAGAAACCTACTTGCTTGGTTGAACTCACTGGTTATGATTTGATTGGCCTTCCATTGAAGTCACCACTATCACTGAATGAGATTATTTATGCTCTTCCTATGTTGACTGTCCTAACAGACAAAGGTACTGGGATTGTGACCAGTGTACCTACTGATTCTCCAGATGATTATATGGCCTTGCATGACTTGAAATCAAAACCTGCTTTTAGGGCAAAATATGGTGTGAAAGATGAGTGGGTACTGCCCTTTGAGAATATCCCAATCATTGATATTCCAGGATTTGGAAATAATGCTGCAGAAAAGGTCTGTGCAGATCTCAAGATCAAAAGCCAGAATGAGAAAGAGAAGCTAGCAGAAGCCAAGAGGTTGACATACTTGAAAGGATTCAATGAAGGAACAATGATTTTGGGGGAGTTTAAGGGAAGGAAAGTCCAGGAGGTTAAACCCTTGATAAGGAGCAAGCTTATAGAGGCAGGAGAGGCAATATCATATAGTGAACCTGAGAAGCGAGTACAATCACGATCCGGTGATGAATGTGTTGTTGCGCTTACAGATCAATGGTACATCACATACGGGGAAACTGAATGGAAAAAACTGGCTGAAGAGTGCTTGTCCGGCATGAATTTGTATTCTGATGAGACACGGCATGGATTTGAACACACATTGAGCTGGTTGAATCAGTGGGCTTGTTCACGATCTTTTGGTCTTGGGACTCGCATCCCCTGGGATGAAGAATTCTTAGTTGAGTCATTGTCTGATTCAACTATTTACATGGCTTATTACACCATTGCTCACTTCCTACACAATGGAGACATGTATGGTTCCAGCAAATCTGCAATTAAACCTGAACAAATGACTGATGAGGTCTGGGATTATGTTTTCTGTGATGGCCCATATCCTAAATCATCTGAGATCTCATCATCAATTCTGAGTAAGATGAAGCAGGAGTTTGAATATTGGTATCCTTTTGATCTTCGGGTGTCTGGCAAGGATCTAATACAAAATCATTTAACCTTCTGCATCTACAATCATACAGCTATTATGTCCAAGAAGCACTGGCCTCGTGGTTTCAGATGCAATGGGCACATAATGCTCAATGCTCAGAAAATGTCCAAGTCTACAGGGAACTTTAGGACAATTCGCCAGGCAATTGAGGAATTTTCTGCTGATGCCACTCGCTTTTCTTTGGCTGATGCTGGTGATGGTGTTGATGATGCAAATTTTGCATTTGACACTGCCAATGCTGCAATCCTGCGGCTCACTAAAGAGATCTCATGGACAGAAGAAGTTTTGGGTGCAGACTCTTCTTTGAGACCAGGCCCCCCAACTACTTATGCTGATAGGGTCTTTGCGAATGAGATAAATATTGCTGTGAATAGGACTGAGCAGAATTACAGCAGTTACATGTTCCGAGAAGCCCTCAAGACTGGATTTTATGATCTTCAAGCTGCTAGGGATGAGTACCGGTTTTCATGTGGTGATGGGGGAATGAACCGTGATTTGGTGTGGCGTTTTATGGATGTGCAGACACGTCTTATTACTCCAATCTGCCCACACTATGGAGAATACGTATGGAGGGAACTTTTGAAGAAGGACGGGTTTGTGGTAAACGCAGGATGGCCTTTGGCTGACTCTCCGGACCTAACCCTCCAGAGTGCCAATAAGTATTTGCAGGACTCAATCATTTTAATGAGGAAGCTCCTCCAAAAGCAAGTTTTAGGCTCAAAGAAGGGTAATAAGAAGGGTACTCCAGTTACAGCGTTGACAGAAGACCACAATCTAACTGGCTTAATCTATGTGAATGAGCACTTTGATGGATGGAAGGCAGAGTGCCTGAAAATCCTCCAAATTAACTTTGACAGAGACAGTTGTACTTTTTCTTCAGACAAGGTGATGCAAGAAGCATTGCAAAAAAGTTCTATTGGTCAGTCGAAAGATTTTAGACAAATCCAAAAGCTTTGTATGCCTTTCATGAAATTCAAGAAGGATGAGGCAATTGCAATTGGGGCTCAGGCCTTGGATTTGAAGCTGCCTTTTGGCGAGATTGAGGTCCTTCAAGAGAACTTGGACTTAATTAAGAGACAACTTGGTCTTCAAGAGGTAGAAATATTGTCTGCTACAAATCCTGATGCACTTTCTAAAGCCGGTTCTCTTGTCAAACTGTTAGAGCAGAATCCTCCAGCACCAGGAAGCCCTACTGCCATCTTCTTGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

1109

Amino Acids

125.65

Weight (kDa)

6.33

Isoelectric Point (pI)

46.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1 PF00133 47 - 127 2.7e-08 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1 PF00133 216 - 778 6.2e-17 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g PF09334 683 - 784 1.2e-08 tRNA synthetases class I (M)
tRNA-synt_1e PF01406 692 - 768 9.3e-09 tRNA synthetases class I (C) catalytic domain
Anticodon_1 PF08264 818 - 947 2.2e-13 Anticodon-binding domain of tRNA ligase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000598)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09620
fragaria_vesca FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_3g34581 FvH4_4g33720 FvH4_4g33720
malus_domestica MD08G1244600.v1.1 MD15G1439400.v1.1
prunus_persica Prupe.1G582000_v2.0.a1 Prupe.2G015100_v2.0.a1 Prupe.2G123100_v2.0.a1
pyrus_communis pycom08g21210 pycom08g21220 pycom15g38730
rosa_chinensis RchiOBHm_Chr1g0336121 RchiOBHm_Chr1g0353811 RchiOBHm_Chr1g0373331 RchiOBHm_Chr4g0443191 RchiOBHm_Chr4g0443201 RchiOBHm_Chr4g0443211
rosa_laevigata RLG00000003122 RLG00000005948 RLG00000026826 RLG00000028251 RLG00000028252 RLG00000028253 RLG00000029377
rosa_multiflora Rmu_co8002498.1_g000001 Rmu_co8262279.1_g000001 Rmu_co8314139.1_g000001 Rmu_co8393711.1_g000001 Rmu_co8419167.1_g000001 Rmu_sc0007968.1_g000004 Rmu_sc0018919.1_g000004 Rmu_sc0027447.1_g000001
rosa_roxburghii Rroxscaffold_4G00284260 Rroxscaffold_4G00301490 Rroxscaffold_4G00315860 Rroxscaffold_5G00383790 Rroxscaffold_5G00383800
rosa_rugosa Rorug01G0123100 Rorug01G0238700 Rorug01G0377100 Rorug01G0377100 Rorug01G0377200 Rorug01G0377300 Rorug04G0344700 Rorug05G0347900.1
rosa_samantha Rh1AG143800 Rh1AG250300 Rh1AG386300 Rh1BG113400 Rh1BG220800 Rh1BG220900 Rh1BG350400 Rh1CG135500 Rh1CG233100 Rh1CG363500 Rh1DG150200 Rh1DG247700 Rh1DG247800 Rh1DG381300 Rh4AG399400 Rh4AG399500 Rh4BG411000 Rh4BG411100 Rh4BG411200 Rh4CG426500 Rh4CG426600 Rh4DG406100 Rh4DG406200 Rh4DG406300
rosa_wichuraiana Rw0G015190 Rw1G021850 Rw1G034870 Rw4G034440 Rw4G034450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 618
AarI CACCTGC 1 cut(s) 473
AasI GACNNNNNNGTC 1 cut(s) 3201
Acc36I ACCTGC 3 cut(s) 473, 736, 1321
AccB1I GGYRCC 2 cut(s) 901, 1025
AccB7I CCANNNNNTGG 2 cut(s) 1394, 2672
AccI GTMKAC 1 cut(s) 2231
AccIII TCCGGA 1 cut(s) 2749
AciI CCGC 5 cut(s) 74, 84, 293, 372, 2368
AclWI GGATC 4 cut(s) 605, 618, 1629, 2112
AcsI RAATTY 7 cut(s) 23, 602, 1735, 1847, 2270, 2332, 3089
AcuI CTGAAG 2 cut(s) 1734, 2979
AcyI GRCGYC 1 cut(s) 729
AfiI CCNNNNNNNGG 9 cut(s) 197, 738, 815, 920, 1394, 1566, 2614, 2672, 3299
AflIII ACRYGT 3 cut(s) 1926, 2517, 2642
AgeI ACCGGT 1 cut(s) 2577
AhlI ACTAGT 1 cut(s) 59
AjiI CACGTC 1 cut(s) 2645
AjnI CCWGG 9 cut(s) 98, 190, 672, 1387, 1545, 1835, 2256, 2428, 3298
AjuI GAANNNNNNNTTGG 2 cut(s) 2597, 2629
Alw21I GWGCWC 3 cut(s) 327, 490, 2922
Alw26I GTCTC 9 cut(s) 99, 682, 961, 968, 1745, 1917, 2419, 2970, 3187
Alw44I GTGCAC 1 cut(s) 486
AlwI GGATC 4 cut(s) 605, 618, 1629, 2112
Aor13HI TCCGGA 1 cut(s) 2749
ApaLI GTGCAC 1 cut(s) 486
ApeKI GCWGC 9 cut(s) 290, 524, 953, 956, 1073, 1406, 2357, 2561, 3142
ApoI RAATTY 7 cut(s) 23, 602, 1735, 1847, 2270, 2332, 3089
AsiGI ACCGGT 1 cut(s) 2577
Asp700I GAANNNNTTC 2 cut(s) 858, 1496
AspLEI GCGC 2 cut(s) 317, 1660
AspS9I GGNCC 8 cut(s) 194, 655, 843, 1108, 2003, 2432, 2752, 3163
AsuC2I CCSGG 1 cut(s) 198
AsuHPI GGTGA 6 cut(s) 863, 1167, 1652, 2324, 2603, 3016
AsuNHI GCTAGC 1 cut(s) 1462
AvaII GGWCC 6 cut(s) 194, 655, 843, 1108, 2752, 3163
BaeGI GKGCMC 2 cut(s) 490, 2199
BaeI ACNNNNGTAYC 4 cut(s) 1242, 1275, 2850, 2883
BamHI GGATCC 1 cut(s) 610
BanI GGYRCC 2 cut(s) 901, 1025
BanII GRGCYC 1 cut(s) 3124
BarI GAAGNNNNNNTAC 2 cut(s) 2977, 3009
BauI CACGAG 1 cut(s) 2175
BbsI GAAGAC 2 cut(s) 2889, 3197
Bbv12I GWGCWC 3 cut(s) 327, 490, 2922
BbvI GCAGC 9 cut(s) 277, 511, 940, 968, 1085, 1393, 2344, 2548, 3129
BceAI ACGGC 1 cut(s) 1772
BcgI CGANNNNNNTGC 2 cut(s) 2156, 2190
BciT130I CCWGG 9 cut(s) 100, 192, 674, 1389, 1547, 1837, 2258, 2430, 3300
BciVI GTATCC 1 cut(s) 2085
BcnI CCSGG 1 cut(s) 198
BcoDI GTCTC 9 cut(s) 99, 682, 961, 968, 1745, 1917, 2419, 2970, 3187
BcuI ACTAGT 1 cut(s) 59
BfaI CTAG 5 cut(s) 60, 387, 1104, 1463, 2565
BfmI CTRYAG 4 cut(s) 705, 954, 1407, 2232
BfuAI ACCTGC 3 cut(s) 473, 736, 1321
BfuI GTATCC 1 cut(s) 2085
BglII AGATCT 4 cut(s) 784, 1426, 2025, 2382
BmcAI AGTACT 1 cut(s) 1100
Bme18I GGWCC 6 cut(s) 194, 655, 843, 1108, 2752, 3163
BmgBI CACGTC 1 cut(s) 2645
BmgT120I GGNCC 8 cut(s) 194, 655, 843, 1108, 2003, 2432, 2752, 3163
BmiI GGNNCC 7 cut(s) 612, 903, 1027, 1109, 1110, 1936, 2434
BmrI ACTGGG 1 cut(s) 1251
BmtI GCTAGC 1 cut(s) 1466
BmuI ACTGGG 1 cut(s) 1251
BpiI GAAGAC 2 cut(s) 2889, 3197
BplI GAGNNNNNCTC 2 cut(s) 2517, 2549
BpmI CTGGAG 7 cut(s) 302, 477, 706, 1257, 2747, 2847, 3276
Bpu10I CCTNAGC 1 cut(s) 3123
BpuEI CTTGAG 4 cut(s) 644, 852, 1415, 2519
BpuMI CCSGG 1 cut(s) 198
BsaAI YACGTR 2 cut(s) 1079, 2683
BsaHI GRCGYC 1 cut(s) 729
BsaI GGTCTC 4 cut(s) 99, 961, 968, 2419
BsaJI CCNNGG 7 cut(s) 197, 468, 673, 765, 1835, 1836, 3129
BsaWI WCCGGW 3 cut(s) 1637, 2577, 2749
BsaXI ACNNNNNCTCC 4 cut(s) 1255, 1285, 2667, 2697
Bsc4I CCNNNNNNNGG 9 cut(s) 197, 738, 815, 920, 1394, 1566, 2614, 2672, 3299
Bse118I RCCGGY 4 cut(s) 108, 539, 2577, 3257
Bse3DI GCAATG 3 cut(s) 1899, 2197, 3017
BseAI TCCGGA 1 cut(s) 2749
BseBI CCWGG 9 cut(s) 100, 192, 674, 1389, 1547, 1837, 2258, 2430, 3300
BseDI CCNNGG 7 cut(s) 197, 468, 673, 765, 1835, 1836, 3129
BseGI GGATG 9 cut(s) 478, 820, 1830, 1846, 2575, 2638, 2735, 2936, 3106
BseLI CCNNNNNNNGG 9 cut(s) 197, 738, 815, 920, 1394, 1566, 2614, 2672, 3299
BseMI GCAATG 3 cut(s) 1899, 2197, 3017
BseMII CTCAG 9 cut(s) 956, 963, 1079, 1605, 2013, 2033, 2228, 2487, 3137
BseRI GAGGAG 3 cut(s) 164, 449, 2804
BseSI GKGCMC 2 cut(s) 490, 2199
BseXI GCAGC 9 cut(s) 277, 511, 940, 968, 1085, 1393, 2344, 2548, 3129
BsgI GTGCAG 4 cut(s) 849, 1443, 2430, 2657
BshNI GGYRCC 2 cut(s) 901, 1025
BshTI ACCGGT 1 cut(s) 2577
BsiHKAI GWGCWC 3 cut(s) 327, 490, 2922
BsiSI CCGG 8 cut(s) 109, 197, 540, 1638, 1728, 2578, 2750, 3258
BslFI GGGAC 2 cut(s) 1094, 1837
BslI CCNNNNNNNGG 9 cut(s) 197, 738, 815, 920, 1394, 1566, 2614, 2672, 3299
BsmAI GTCTC 9 cut(s) 99, 682, 961, 968, 1745, 1917, 2419, 2970, 3187
BsmBI CGTCTC 1 cut(s) 682
BsmFI GGGAC 2 cut(s) 1094, 1837
Bso31I GGTCTC 4 cut(s) 99, 961, 968, 2419
Bsp1286I GDGCHC 5 cut(s) 327, 490, 2199, 2922, 3124
Bsp13I TCCGGA 1 cut(s) 2749
Bsp19I CCATGG 1 cut(s) 765
BspACI CCGC 5 cut(s) 74, 84, 293, 372, 2368
BspCNI CTCAG 9 cut(s) 957, 964, 1078, 1606, 2014, 2034, 2227, 2488, 3136
BspEI TCCGGA 1 cut(s) 2749
BspHI TCATGA 2 cut(s) 835, 3084
BspLI GGNNCC 7 cut(s) 612, 903, 1027, 1109, 1110, 1936, 2434
BspMAI CTGCAG 2 cut(s) 958, 1411
BspMI ACCTGC 3 cut(s) 473, 736, 1321
BspOI GCTAGC 1 cut(s) 1466
BspPI GGATC 4 cut(s) 605, 618, 1629, 2112
BspQI GCTCTTC 2 cut(s) 83, 1212
BspT107I GGYRCC 2 cut(s) 901, 1025
BspTNI GGTCTC 4 cut(s) 99, 961, 968, 2419
BsrDI GCAATG 3 cut(s) 1899, 2197, 3017
BsrFI RCCGGY 4 cut(s) 108, 539, 2577, 3257
BssAI RCCGGY 4 cut(s) 108, 539, 2577, 3257
BssECI CCNNGG 7 cut(s) 197, 468, 673, 765, 1835, 1836, 3129
BssNI GRCGYC 1 cut(s) 729
BssSI CACGAG 1 cut(s) 2175
BssT1I CCWWGG 2 cut(s) 765, 3129
Bst2BI CACGAG 1 cut(s) 2175
Bst2UI CCWGG 9 cut(s) 100, 192, 674, 1389, 1547, 1837, 2258, 2430, 3300
Bst6I CTCTTC 4 cut(s) 83, 1212, 1710, 2422
BstACI GRCGYC 1 cut(s) 729
BstAPI GCANNNNNTGC 2 cut(s) 2203, 2336
BstBAI YACGTR 2 cut(s) 1079, 2683
BstC8I GCNNGC 3 cut(s) 356, 1464, 1574
BstDSI CCRYGG 1 cut(s) 765
BstENI CCTNNNNNAGG 2 cut(s) 736, 1564
BstF5I GGATG 9 cut(s) 478, 820, 1830, 1846, 2575, 2638, 2735, 2936, 3106
BstHHI GCGC 2 cut(s) 317, 1660
BstMAI GTCTC 9 cut(s) 99, 682, 961, 968, 1745, 1917, 2419, 2970, 3187
BstNI CCWGG 9 cut(s) 100, 192, 674, 1389, 1547, 1837, 2258, 2430, 3300
BstNSI RCATGY 4 cut(s) 263, 358, 1930, 2521
BstSFI CTRYAG 4 cut(s) 705, 954, 1407, 2232
BstSLI GKGCMC 2 cut(s) 490, 2199
BstSNI TACGTA 1 cut(s) 2683
BstV1I GCAGC 9 cut(s) 277, 511, 940, 968, 1085, 1393, 2344, 2548, 3129
BstV2I GAAGAC 2 cut(s) 2889, 3197
BstX2I RGATCY 6 cut(s) 610, 784, 1426, 2025, 2104, 2382
BstYI RGATCY 6 cut(s) 610, 784, 1426, 2025, 2104, 2382
BsuI GTATCC 1 cut(s) 2085
BtgI CCRYGG 1 cut(s) 765
BtrI CACGTC 1 cut(s) 2645
BtsCI GGATG 9 cut(s) 478, 820, 1830, 1846, 2575, 2638, 2735, 2936, 3106
BtsI GCAGTG 2 cut(s) 654, 2346
BveI ACCTGC 3 cut(s) 473, 736, 1321
Cac8I GCNNGC 3 cut(s) 356, 1464, 1574
CciI TCATGA 2 cut(s) 835, 3084
CfoI GCGC 2 cut(s) 317, 1660
Cfr10I RCCGGY 4 cut(s) 108, 539, 2577, 3257
Cfr13I GGNCC 8 cut(s) 194, 655, 843, 1108, 2003, 2432, 2752, 3163
CseI GACGC 1 cut(s) 737
CspAI ACCGGT 1 cut(s) 2577
DrdI GACNNNNNNGTC 1 cut(s) 3201
DseDI GACNNNNNNGTC 1 cut(s) 3201
Eam1104I CTCTTC 4 cut(s) 83, 1212, 1710, 2422
EarI CTCTTC 4 cut(s) 83, 1212, 1710, 2422
EciI GGCGGA 2 cut(s) 89, 361
Eco105I TACGTA 1 cut(s) 2683
Eco130I CCWWGG 2 cut(s) 765, 3129
Eco147I AGGCCT 2 cut(s) 368, 3128
Eco24I GRGCYC 1 cut(s) 3124
Eco31I GGTCTC 4 cut(s) 99, 961, 968, 2419
Eco47I GGWCC 6 cut(s) 194, 655, 843, 1108, 2752, 3163
Eco57I CTGAAG 2 cut(s) 1734, 2979
EcoNI CCTNNNNNAGG 2 cut(s) 736, 1564
EcoO109I RGGNCCY 4 cut(s) 655, 1108, 2432, 3163
EcoRI GAATTC 1 cut(s) 1847
EcoRII CCWGG 9 cut(s) 98, 190, 672, 1387, 1545, 1835, 2256, 2428, 3298
EcoT14I CCWWGG 2 cut(s) 765, 3129
EcoT22I ATGCAT 1 cut(s) 1000
EcoT38I GRGCYC 1 cut(s) 3124
ErhI CCWWGG 2 cut(s) 765, 3129
Esp3I CGTCTC 1 cut(s) 682
FalI AAGNNNNNCTT 2 cut(s) 933, 965
FaqI GGGAC 2 cut(s) 1094, 1837
FauI CCCGC 1 cut(s) 77
FblI GTMKAC 1 cut(s) 2231
FokI GGATG 9 cut(s) 485, 827, 1817, 1853, 2582, 2645, 2742, 2943, 3113
FriOI GRGCYC 1 cut(s) 3124
FspBI CTAG 5 cut(s) 60, 387, 1104, 1463, 2565
GlaI GCGC 2 cut(s) 316, 1659
GsuI CTGGAG 7 cut(s) 302, 477, 706, 1257, 2747, 2847, 3276
HapII CCGG 8 cut(s) 109, 197, 540, 1638, 1728, 2578, 2750, 3258
HgaI GACGC 1 cut(s) 737
HhaI GCGC 2 cut(s) 317, 1660
Hin1I GRCGYC 1 cut(s) 729
Hin6I GCGC 2 cut(s) 315, 1658
HinP1I GCGC 2 cut(s) 315, 1658
HincII GTYRAC 4 cut(s) 627, 1218, 1482, 2877
HindII GTYRAC 4 cut(s) 627, 1218, 1482, 2877
HindIII AAGCTT 4 cut(s) 380, 571, 1574, 3068
HpaII CCGG 8 cut(s) 109, 197, 540, 1638, 1728, 2578, 2750, 3258
HphI GGTGA 6 cut(s) 863, 1167, 1652, 2324, 2603, 3016
Hpy99I CGWCG 1 cut(s) 731
HpyCH4IV ACGT 4 cut(s) 691, 1078, 2644, 2682
HpySE526I ACGT 4 cut(s) 691, 1078, 2644, 2682
Hsp92I GRCGYC 1 cut(s) 729
HspAI GCGC 2 cut(s) 315, 1658
KflI GGGWCCC 1 cut(s) 1108
Kpn2I TCCGGA 1 cut(s) 2749
LguI GCTCTTC 2 cut(s) 83, 1212
LmnI GCTCC 3 cut(s) 330, 1569, 2817
Lsp1109I GCAGC 9 cut(s) 277, 511, 940, 968, 1085, 1393, 2344, 2548, 3129
MaeI CTAG 5 cut(s) 60, 387, 1104, 1463, 2565
MaeII ACGT 4 cut(s) 691, 1078, 2644, 2682
MaeIII GTNAC 7 cut(s) 590, 680, 791, 1173, 1249, 2513, 2866
MfeI CAATTG 3 cut(s) 2262, 3108, 3114
MflI RGATCY 6 cut(s) 610, 784, 1426, 2025, 2104, 2382
MhlI GDGCHC 5 cut(s) 327, 490, 2199, 2922, 3124
MlyI GAGTC 6 cut(s) 182, 1819, 1868, 2408, 2738, 2783
MmeI TCCRAC 2 cut(s) 609, 909
Mph1103I ATGCAT 1 cut(s) 1000
MroI TCCGGA 1 cut(s) 2749
MroXI GAANNNNTTC 2 cut(s) 858, 1496
MslI CAYNNNNRTG 3 cut(s) 887, 2801, 2925
MspI CCGG 8 cut(s) 109, 197, 540, 1638, 1728, 2578, 2750, 3258
MunI CAATTG 3 cut(s) 2262, 3108, 3114
MvaI CCWGG 9 cut(s) 100, 192, 674, 1389, 1547, 1837, 2258, 2430, 3300
NciI CCSGG 1 cut(s) 198
NcoI CCATGG 1 cut(s) 765
NheI GCTAGC 1 cut(s) 1462
NlaIV GGNNCC 7 cut(s) 612, 903, 1027, 1109, 1110, 1936, 2434
NmeAIII GCCGAG 1 cut(s) 493
NmuCI GTSAC 4 cut(s) 590, 680, 1173, 1249
NsiI ATGCAT 1 cut(s) 1000
NspI RCATGY 4 cut(s) 263, 358, 1930, 2521
PacI TTAATTAA 1 cut(s) 3189
PaeI GCATGC 1 cut(s) 358
PagI TCATGA 2 cut(s) 835, 3084
PaqCI CACCTGC 1 cut(s) 473
PasI CCCWGGG 1 cut(s) 1836
PceI AGGCCT 2 cut(s) 368, 3128
PciI ACATGT 2 cut(s) 1926, 2517
PciSI GCTCTTC 2 cut(s) 83, 1212
PdmI GAANNNNTTC 2 cut(s) 858, 1496
PfeI GAWTC 7 cut(s) 394, 715, 1268, 1497, 1789, 1871, 3286
PflMI CCANNNNNTGG 2 cut(s) 1394, 2672
PfoI TCCNGGA 3 cut(s) 190, 1387, 1545
PinAI ACCGGT 1 cut(s) 2577
PleI GAGTC 6 cut(s) 181, 1819, 1867, 2408, 2738, 2783
PpsI GAGTC 6 cut(s) 181, 1819, 1867, 2408, 2738, 2783
Ppu21I YACGTR 2 cut(s) 1079, 2683
PpuMI RGGWCCY 3 cut(s) 655, 1108, 3163
PscI ACATGT 2 cut(s) 1926, 2517
PsiI TTATAA 1 cut(s) 618
Psp5II RGGWCCY 3 cut(s) 655, 1108, 3163
Psp6I CCWGG 9 cut(s) 98, 190, 672, 1387, 1545, 1835, 2256, 2428, 3298
PspGI CCWGG 9 cut(s) 98, 190, 672, 1387, 1545, 1835, 2256, 2428, 3298
PspN4I GGNNCC 7 cut(s) 612, 903, 1027, 1109, 1110, 1936, 2434
PspPI GGNCC 8 cut(s) 194, 655, 843, 1108, 2003, 2432, 2752, 3163
PspPPI RGGWCCY 3 cut(s) 655, 1108, 3163
PstI CTGCAG 2 cut(s) 958, 1411
PsuI RGATCY 6 cut(s) 610, 784, 1426, 2025, 2104, 2382
RseI CAYNNNNRTG 3 cut(s) 887, 2801, 2925
SapI GCTCTTC 2 cut(s) 83, 1212
Sau96I GGNCC 8 cut(s) 194, 655, 843, 1108, 2003, 2432, 2752, 3163
ScaI AGTACT 1 cut(s) 1100
SchI GAGTC 6 cut(s) 182, 1819, 1868, 2408, 2738, 2783
SduI GDGCHC 5 cut(s) 327, 490, 2199, 2922, 3124
SfcI CTRYAG 4 cut(s) 705, 954, 1407, 2232
SinI GGWCC 6 cut(s) 194, 655, 843, 1108, 2752, 3163
SmiMI CAYNNNNRTG 3 cut(s) 887, 2801, 2925
SmlI CTYRAG 4 cut(s) 659, 867, 1430, 2534
SmoI CTYRAG 4 cut(s) 659, 867, 1430, 2534
SnaBI TACGTA 1 cut(s) 2683
SpeI ACTAGT 1 cut(s) 59
SphI GCATGC 1 cut(s) 358
SseBI AGGCCT 2 cut(s) 368, 3128
SsiI CCGC 5 cut(s) 74, 84, 293, 372, 2368
SspI AATATT 3 cut(s) 2069, 2479, 3222
SspMI CTAG 5 cut(s) 60, 387, 1104, 1463, 2565
StuI AGGCCT 2 cut(s) 368, 3128
StyI CCWWGG 2 cut(s) 765, 3129
TaiI ACGT 4 cut(s) 694, 1081, 2647, 2685
TaqI TCGA 5 cut(s) 123, 136, 518, 726, 3044
TatI WGTACW 4 cut(s) 347, 1098, 1624, 2984
TauI GCSGC 2 cut(s) 296, 2371
TfiI GAWTC 7 cut(s) 394, 715, 1268, 1497, 1789, 1871, 3286
TseFI GTSAC 4 cut(s) 590, 680, 1173, 1249
TseI GCWGC 9 cut(s) 290, 524, 953, 956, 1073, 1406, 2357, 2561, 3142
Tsp45I GTSAC 4 cut(s) 590, 680, 1173, 1249
TspGWI ACGGA 1 cut(s) 779
Van91I CCANNNNNTGG 2 cut(s) 1394, 2672
VneI GTGCAC 1 cut(s) 486
VpaK11BI GGWCC 6 cut(s) 194, 655, 843, 1108, 2752, 3163
XagI CCTNNNNNAGG 2 cut(s) 736, 1564
XapI RAATTY 7 cut(s) 23, 602, 1735, 1847, 2270, 2332, 3089
XceI RCATGY 4 cut(s) 263, 358, 1930, 2521
XcmI CCANNNNNNNNNTGG 1 cut(s) 2894
XmiI GTMKAC 1 cut(s) 2231
XmnI GAANNNNTTC 2 cut(s) 858, 1496
XspI CTAG 5 cut(s) 60, 387, 1104, 1463, 2565
ZrmI AGTACT 1 cut(s) 1100
Zsp2I ATGCAT 1 cut(s) 1000
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.