Rw4G034440

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000598
Physical Location & Seq
Reverse (-)
61512606 .. 61516713
4108 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G034440.1

Sequence Viewer

Length: 2295 bp
ATGTGTTCATCGATTCGGATTCTCTCTTACTTCCACACTTTTGATCTTATTGTTAATGTCATCTTTCTGGTGTTTGATGGTTTGTTTAATTTGACAAACAATTTTACTCAAAAGGTATCCAAAGGTGATAACATTCTCCTAGAGATTAAGATTCTCCTAGAGATTGAAGCAGAGGTTCGAGAATGGTGGGAAGAGAAAGGCGTTTTTGTCAATGCTGATCAATCTCCTGACAACCCTCACTATCCTAACGATGAACTGGGGGATGATCGGAAGAAGTGGTTTGAAAACTATCTATTTGGTGGTTTATCGCGACGGGCATTCCTGATATCTAAGCTAGAGTGTTGTGCAGCTTATCATAGATTAAGAGATGACAATGTGCCATGGCAAATCAAAGCCTCAGGCGCAGAGGAACTTCCGGAGAAGATTATAAATCTGTTGGTTGGAGAACCACCAGTATTTCCAACAGTTTGGAGAGCTGCCTATTCTACTGATGCCATGAGACTCTCCTTAGCTTGTTCTTGTGATGGTGATGATTCTCCGAGAATTTTATTTAATACTGCAAATTCTGCTATCCGAAAGCTCACTAAAGAAATGTCATGGATTAGTGAACAACTAGCTTATATGGCTGCTGCTGCAGACTCTTCTTCTTTTGTGAGAAGAATTAGAAGAGATGGCCCCCCCAGTACTTTTGCGGACAAGGTGTTTGCGAATGAGATAAATATTGCTGTGCATAACAGTGATAACTATTACCGGGCTTGCATGTTTCAAGAAGCCCTCATTTCCGGCTTTCTTCAACTTCAGGCTGCCAGGGACTCGTATAGAATTTCATGTGGTCCTCATGGCATGAACCATGATTTGGTGCTGCGCTATATTGATGTGCATACACGTCTAATTGCTCCAATCTGTCCACACTATGCTGAATATGTCTGGCGGGAATATTTAAAAAAGGAGGGGTTTGTGGTAATTAAGTCAGGCTGGCCTGCCGCTGATGCCCCGGATTTGATACTCCAAAGTGCCAATAAGTATTTGCAAGATCTAATTGAACTTATGAGGGAGCTTGAAAAGAAGTTTCGAGCTTTAAATGATGGTAATTATGATGTGACAGAAGAAGAAACGAAGGCAGTAGGTTTGATATATGTTAAGGAGGAAATTGATGGATGGGAGGGTAAGTGCTTGAGGATACTTCAAGATAACTTTGACAGCGAGACAAACACCTTTTCTGCTCAGGACGAGGACATGCTAGACGCATTAATATCGTACATATATGGGGATTTTACCATTCGTCAGGATATAGATTATAGGCAAACTGAACAGCTCTGTATGTCTTTTCTGAAGTTGAAGAAGGATGAAGCACTTAATTTTGGGGCTGATGCCTTAGAGTTGAAGCTACCATTTGGAGAGATTGAGGTCGTTCAAGAAAACTTGGACTTGATTAAGAGAGAAATTGGCCTTCATGAGGTTAAGGTACAAGTTTTCTCTGGTACCACCGGTCTTGAAGATTTTACACTGGATAGTCCTCCATCCCCTGGAAGGCCAGCTGCTGCCTTGGTGAGCCGTGACTTCTATGAGAAAATGGAACGTTCTGCTCCTGGAGTATGGGAACTGAAACGTCGTCTTCAGTTATCAGATAAAGTTCTTACTCCCTCGTCTCGTCCTCCCTCTGAGCTATTGTACCAGAAGAAAATGGAACATCCGTTTAAGTTGTTATTCCAGGAGAAAACGGAACATTCTGACCTTGGAGAACAGGAACCGAATGATGAACAATCCCCAGATAATATTCTTGCTCCATTATATCATCCTCCCTATGAGCTATTGTTTCAGGGCTCTTTCAAAAAGGCCAAACGTACTGCTTCTGCCGAGGACAAATGGCTCCTCGTGAACTTGCAATCGACTAAGGAATTCAGCTCACATCTGATTAATCAAGACACCTGGGCTAATGAAGCTGTCTCGAAGATCATCAGCACTAATTTTATCTTCTGGCAGGCGTATGATGATTCAAATGAAGTCAAGAAGATTTGCAGATTCTACAACTTGGTATATACACCTGTAGTGCTTATCATTGACCCCATAAATGGTGAGAATATGCGTTCGTGGTATGGAATGGTTCAACCAGAACGTCTGCTGGAGGATCTGTTAGTACCATTTTTGGAAAATAGCCCCAAGGATCACCATCATCCAGGAGAAGAAAGTTCTCAGCCTCAGCCAGAGGAAAGTGAAGATGATTCGGTTGGTGACAAAACTGATGAAGAAGATGAGGAATTGCAGTGGGCATTGGAAGCTTCCATGAAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

764

Amino Acids

87.7

Weight (kDa)

4.81

Isoelectric Point (pI)

57.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anticodon_1 PF08264 232 - 357 8.6e-11 Anticodon-binding domain of tRNA ligase
Thioredoxin_7 PF13899 609 - 689 1.3e-12 Thioredoxin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000598)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09620
fragaria_vesca FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_3g34581 FvH4_4g33720 FvH4_4g33720
malus_domestica MD08G1244600.v1.1 MD15G1439400.v1.1
prunus_persica Prupe.1G582000_v2.0.a1 Prupe.2G015100_v2.0.a1 Prupe.2G123100_v2.0.a1
pyrus_communis pycom08g21210 pycom08g21220 pycom15g38730
rosa_chinensis RchiOBHm_Chr1g0336121 RchiOBHm_Chr1g0353811 RchiOBHm_Chr1g0373331 RchiOBHm_Chr4g0443191 RchiOBHm_Chr4g0443201 RchiOBHm_Chr4g0443211
rosa_laevigata RLG00000003122 RLG00000005948 RLG00000026826 RLG00000028251 RLG00000028252 RLG00000028253 RLG00000029377
rosa_multiflora Rmu_co8002498.1_g000001 Rmu_co8262279.1_g000001 Rmu_co8314139.1_g000001 Rmu_co8393711.1_g000001 Rmu_co8419167.1_g000001 Rmu_sc0007968.1_g000004 Rmu_sc0018919.1_g000004 Rmu_sc0027447.1_g000001
rosa_roxburghii Rroxscaffold_4G00284260 Rroxscaffold_4G00301490 Rroxscaffold_4G00315860 Rroxscaffold_5G00383790 Rroxscaffold_5G00383800
rosa_rugosa Rorug01G0123100 Rorug01G0238700 Rorug01G0377100 Rorug01G0377100 Rorug01G0377200 Rorug01G0377300 Rorug04G0344700 Rorug05G0347900.1
rosa_samantha Rh1AG143800 Rh1AG250300 Rh1AG386300 Rh1BG113400 Rh1BG220800 Rh1BG220900 Rh1BG350400 Rh1CG135500 Rh1CG233100 Rh1CG363500 Rh1DG150200 Rh1DG247700 Rh1DG247800 Rh1DG381300 Rh4AG399400 Rh4AG399500 Rh4BG411000 Rh4BG411100 Rh4BG411200 Rh4CG426500 Rh4CG426600 Rh4DG406100 Rh4DG406200 Rh4DG406300
rosa_wichuraiana Rw0G015190 Rw1G021850 Rw1G034870 Rw4G034440 Rw4G034450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 428
Acc65I GGTACC 1 cut(s) 1481
AccB1I GGYRCC 1 cut(s) 1481
AccB7I CCANNNNNTGG 2 cut(s) 856, 1526
AccII CGCG 1 cut(s) 310
AccIII TCCGGA 1 cut(s) 415
AciI CCGC 3 cut(s) 692, 931, 984
AclI AACGTT 1 cut(s) 1579
AclWI GGATC 2 cut(s) 2136, 2172
AcsI RAATTY 4 cut(s) 543, 562, 822, 1898
AcuI CTGAAG 3 cut(s) 782, 1352, 1601
AfaI GTAC 7 cut(s) 685, 1259, 1467, 1483, 1673, 1846, 2139
AfiI CCNNNNNNNGG 5 cut(s) 856, 1456, 1526, 1530, 2072
AflIII ACRYGT 1 cut(s) 884
AgeI ACCGGT 1 cut(s) 1487
AjiI CACGTC 1 cut(s) 887
AjnI CCWGG 6 cut(s) 806, 1525, 1588, 1710, 1928, 2176
AjuI GAANNNNNNNTTGG 2 cut(s) 839, 871
Alw26I GTCTC 4 cut(s) 493, 1199, 1653, 1951
AlwI GGATC 2 cut(s) 2136, 2172
AlwNI CAGNNNCTG 1 cut(s) 1541
Aor13HI TCCGGA 1 cut(s) 415
AoxI GGCC 5 cut(s) 673, 977, 1447, 1532, 1836
ApeKI GCWGC 9 cut(s) 347, 476, 626, 629, 632, 803, 862, 1538, 1541
ApoI RAATTY 4 cut(s) 543, 562, 822, 1898
ArsI GACNNNNNNTTYG 2 cut(s) 686, 718
AseI ATTAAT 2 cut(s) 1250, 1917
AsiGI ACCGGT 1 cut(s) 1487
Asp718I GGTACC 1 cut(s) 1481
AspLEI GCGC 2 cut(s) 404, 867
AspS9I GGNCC 2 cut(s) 674, 833
AsuC2I CCSGG 2 cut(s) 752, 995
AsuHPI GGTGA 6 cut(s) 137, 539, 1561, 2087, 2159, 2243
AvaII GGWCC 1 cut(s) 833
AxyI CCTNAGG 1 cut(s) 397
BanI GGYRCC 1 cut(s) 1481
BanII GRGCYC 1 cut(s) 1826
BauI CACGAG 1 cut(s) 1874
BbsI GAAGAC 1 cut(s) 1607
BbvCI CCTCAGC 1 cut(s) 2199
BbvI GCAGC 9 cut(s) 359, 463, 613, 616, 619, 790, 849, 1525, 1528
BccI CCATC 8 cut(s) 71, 518, 665, 1079, 1148, 1152, 1528, 2178
BceAI ACGGC 1 cut(s) 1539
BcgI CGANNNNNNTGC 2 cut(s) 1236, 1270
BciT130I CCWGG 6 cut(s) 808, 1527, 1590, 1712, 1930, 2178
BciVI GTATCC 2 cut(s) 127, 1173
BclI TGATCA 1 cut(s) 217
BcnI CCSGG 2 cut(s) 752, 995
BcoDI GTCTC 4 cut(s) 493, 1199, 1653, 1951
BfaI CTAG 5 cut(s) 140, 158, 335, 614, 1241
BfmI CTRYAG 2 cut(s) 633, 2046
BfuI GTATCC 2 cut(s) 127, 1173
BglII AGATCT 1 cut(s) 1033
BmcAI AGTACT 1 cut(s) 685
Bme1390I CCNGG 8 cut(s) 752, 808, 995, 1527, 1590, 1712, 1930, 2178
Bme18I GGWCC 1 cut(s) 833
BmgBI CACGTC 1 cut(s) 887
BmgT120I GGNCC 2 cut(s) 674, 833
BmiI GGNNCC 4 cut(s) 676, 1483, 1749, 1871
BmrFI CCNGG 8 cut(s) 752, 808, 995, 1527, 1590, 1712, 1930, 2178
BmrI ACTGGG 2 cut(s) 266, 675
BmsI GCATC 3 cut(s) 481, 979, 1360
BmuI ACTGGG 2 cut(s) 266, 675
BpiI GAAGAC 1 cut(s) 1607
BpmI CTGGAG 2 cut(s) 1611, 2144
Bpu10I CCTNAGC 3 cut(s) 508, 1224, 2199
BpuEI CTTGAG 1 cut(s) 1195
BpuMI CCSGG 2 cut(s) 752, 995
Bsa29I ATCGAT 1 cut(s) 11
BsaBI GATNNNNATC 1 cut(s) 2169
BsaJI CCNNGG 9 cut(s) 380, 807, 993, 1525, 1545, 1735, 1857, 1929, 2160
BsaWI WCCGGW 2 cut(s) 415, 1487
BsaXI ACNNNNNCTCC 4 cut(s) 1154, 1184, 2117, 2147
Bsc4I CCNNNNNNNGG 5 cut(s) 856, 1456, 1526, 1530, 2072
Bse118I RCCGGY 1 cut(s) 1487
Bse1I ACTGG 4 cut(s) 261, 452, 681, 1512
Bse21I CCTNAGG 1 cut(s) 397
Bse8I GATNNNNATC 1 cut(s) 2169
BseAI TCCGGA 1 cut(s) 415
BseBI CCWGG 6 cut(s) 808, 1527, 1590, 1712, 1930, 2178
BseCI ATCGAT 1 cut(s) 11
BseDI CCNNGG 9 cut(s) 380, 807, 993, 1525, 1545, 1735, 1857, 1929, 2160
BseGI GGATG 7 cut(s) 268, 1163, 1351, 1520, 1690, 1795, 2173
BseJI GATNNNNATC 1 cut(s) 2169
BseLI CCNNNNNNNGG 5 cut(s) 856, 1456, 1526, 1530, 2072
BseMII CTCAG 5 cut(s) 411, 1238, 1653, 2207, 2213
BseNI ACTGG 4 cut(s) 261, 452, 681, 1512
BseRI GAGGAG 1 cut(s) 1862
BseXI GCAGC 9 cut(s) 359, 463, 613, 616, 619, 790, 849, 1525, 1528
BsgI GTGCAG 1 cut(s) 366
Bsh1236I CGCG 1 cut(s) 310
BshFI GGCC 5 cut(s) 675, 979, 1449, 1534, 1838
BshNI GGYRCC 1 cut(s) 1481
BshTI ACCGGT 1 cut(s) 1487
BshVI ATCGAT 1 cut(s) 11
BsiSI CCGG 5 cut(s) 416, 751, 783, 995, 1488
BslFI GGGAC 1 cut(s) 824
BslI CCNNNNNNNGG 5 cut(s) 856, 1456, 1526, 1530, 2072
BsmAI GTCTC 4 cut(s) 493, 1199, 1653, 1951
BsmBI CGTCTC 1 cut(s) 1653
BsmFI GGGAC 1 cut(s) 824
BsmI GAATGC 1 cut(s) 317
BsnI GGCC 5 cut(s) 675, 979, 1449, 1534, 1838
Bsp1286I GDGCHC 1 cut(s) 1826
Bsp13I TCCGGA 1 cut(s) 415
Bsp143I GATC 7 cut(s) 43, 217, 265, 1033, 1953, 2128, 2164
Bsp19I CCATGG 1 cut(s) 380
Bsp68I TCGCGA 1 cut(s) 310
BspACI CCGC 3 cut(s) 692, 931, 984
BspANI GGCC 5 cut(s) 675, 979, 1449, 1534, 1838
BspCNI CTCAG 5 cut(s) 410, 1237, 1654, 2206, 2212
BspDI ATCGAT 1 cut(s) 11
BspEI TCCGGA 1 cut(s) 415
BspFNI CGCG 1 cut(s) 310
BspHI TCATGA 1 cut(s) 1453
BspLI GGNNCC 4 cut(s) 676, 1483, 1749, 1871
BspMAI CTGCAG 1 cut(s) 637
BspPI GGATC 2 cut(s) 2136, 2172
BspT107I GGYRCC 1 cut(s) 1481
BsrFI RCCGGY 1 cut(s) 1487
BsrI ACTGG 4 cut(s) 261, 452, 681, 1512
BssAI RCCGGY 1 cut(s) 1487
BssECI CCNNGG 9 cut(s) 380, 807, 993, 1525, 1545, 1735, 1857, 1929, 2160
BssMI GATC 7 cut(s) 43, 217, 265, 1033, 1953, 2128, 2164
BssSI CACGAG 1 cut(s) 1874
BssT1I CCWWGG 4 cut(s) 380, 1545, 1735, 2160
Bst2BI CACGAG 1 cut(s) 1874
Bst2UI CCWGG 6 cut(s) 808, 1527, 1590, 1712, 1930, 2178
Bst4CI ACNGT 2 cut(s) 466, 737
Bst6I CTCTTC 3 cut(s) 186, 646, 661
BstAPI GCANNNNNTGC 1 cut(s) 566
BstC8I GCNNGC 5 cut(s) 757, 977, 981, 1536, 1983
BstDEI CTNAG 9 cut(s) 330, 397, 508, 1224, 1375, 1662, 1893, 2193, 2199
BstDSI CCRYGG 1 cut(s) 380
BstENI CCTNNNNNAGG 1 cut(s) 1454
BstF5I GGATG 7 cut(s) 268, 1163, 1351, 1520, 1690, 1795, 2173
BstFNI CGCG 1 cut(s) 310
BstHHI GCGC 2 cut(s) 404, 867
BstKTI GATC 7 cut(s) 46, 220, 268, 1036, 1956, 2131, 2167
BstMAI GTCTC 4 cut(s) 493, 1199, 1653, 1951
BstMBI GATC 7 cut(s) 43, 217, 265, 1033, 1953, 2128, 2164
BstMWI GCNNNNNNNGC 7 cut(s) 401, 566, 623, 632, 989, 1940, 2276
BstNI CCWGG 6 cut(s) 808, 1527, 1590, 1712, 1930, 2178
BstNSI RCATGY 2 cut(s) 763, 1240
BstSCI CCNGG 8 cut(s) 750, 806, 993, 1525, 1588, 1710, 1928, 2176
BstSFI CTRYAG 2 cut(s) 633, 2046
BstUI CGCG 1 cut(s) 310
BstV1I GCAGC 9 cut(s) 359, 463, 613, 616, 619, 790, 849, 1525, 1528
BstV2I GAAGAC 1 cut(s) 1607
BstX2I RGATCY 2 cut(s) 1033, 2128
BstXI CCANNNNNNTGG 1 cut(s) 468
BstYI RGATCY 2 cut(s) 1033, 2128
Bsu15I ATCGAT 1 cut(s) 11
Bsu36I CCTNAGG 1 cut(s) 397
BsuI GTATCC 2 cut(s) 127, 1173
BsuRI GGCC 5 cut(s) 675, 979, 1449, 1534, 1838
BsuTUI ATCGAT 1 cut(s) 11
BtgI CCRYGG 1 cut(s) 380
BtrI CACGTC 1 cut(s) 887
BtsCI GGATG 7 cut(s) 268, 1163, 1351, 1520, 1690, 1795, 2173
BtsI GCAGTG 1 cut(s) 2270
BtsIMutI CAGTG 3 cut(s) 742, 1505, 2270
BtuMI TCGCGA 1 cut(s) 310
Cac8I GCNNGC 5 cut(s) 757, 977, 981, 1536, 1983
CaiI CAGNNNCTG 1 cut(s) 1541
CciI TCATGA 1 cut(s) 1453
CfoI GCGC 2 cut(s) 404, 867
Cfr10I RCCGGY 1 cut(s) 1487
Cfr13I GGNCC 2 cut(s) 674, 833
ClaI ATCGAT 1 cut(s) 11
CseI GACGC 1 cut(s) 1253
Csp6I GTAC 7 cut(s) 684, 1258, 1466, 1482, 1672, 1845, 2138
CspAI ACCGGT 1 cut(s) 1487
CspCI CAANNNNNGTGG 2 cut(s) 1474, 1509
CviQI GTAC 7 cut(s) 684, 1258, 1466, 1482, 1672, 1845, 2138
DdeI CTNAG 9 cut(s) 330, 397, 508, 1224, 1375, 1662, 1893, 2193, 2199
DpnI GATC 7 cut(s) 45, 219, 267, 1035, 1955, 2130, 2166
DpnII GATC 7 cut(s) 43, 217, 265, 1033, 1953, 2128, 2164
DraI TTTAAA 2 cut(s) 942, 1080
Eam1104I CTCTTC 3 cut(s) 186, 646, 661
EarI CTCTTC 3 cut(s) 186, 646, 661
Eco130I CCWWGG 4 cut(s) 380, 1545, 1735, 2160
Eco24I GRGCYC 1 cut(s) 1826
Eco32I GATATC 1 cut(s) 327
Eco47I GGWCC 1 cut(s) 833
Eco57I CTGAAG 3 cut(s) 782, 1352, 1601
Eco81I CCTNAGG 1 cut(s) 397
EcoNI CCTNNNNNAGG 1 cut(s) 1454
EcoRI GAATTC 1 cut(s) 1898
EcoRII CCWGG 6 cut(s) 806, 1525, 1588, 1710, 1928, 2176
EcoRV GATATC 1 cut(s) 327
EcoT14I CCWWGG 4 cut(s) 380, 1545, 1735, 2160
EcoT38I GRGCYC 1 cut(s) 1826
ErhI CCWWGG 4 cut(s) 380, 1545, 1735, 2160
Esp3I CGTCTC 1 cut(s) 1653
FaqI GGGAC 1 cut(s) 824
FauI CCCGC 1 cut(s) 924
FbaI TGATCA 1 cut(s) 217
FokI GGATG 7 cut(s) 275, 1170, 1358, 1507, 1677, 1782, 2160
FriOI GRGCYC 1 cut(s) 1826
FspBI CTAG 5 cut(s) 140, 158, 335, 614, 1241
GlaI GCGC 2 cut(s) 403, 866
GsuI CTGGAG 2 cut(s) 1611, 2144
HaeIII GGCC 5 cut(s) 675, 979, 1449, 1534, 1838
HapII CCGG 5 cut(s) 416, 751, 783, 995, 1488
HgaI GACGC 1 cut(s) 1253
HhaI GCGC 2 cut(s) 404, 867
Hin6I GCGC 2 cut(s) 402, 865
HinP1I GCGC 2 cut(s) 402, 865
HindIII AAGCTT 1 cut(s) 2277
HpaII CCGG 5 cut(s) 416, 751, 783, 995, 1488
HphI GGTGA 6 cut(s) 137, 539, 1561, 2087, 2159, 2243
Hpy166II GTNNAC 3 cut(s) 608, 908, 1879
Hpy188I TCNGA 9 cut(s) 18, 270, 540, 575, 1332, 1627, 1663, 1732, 1914
Hpy8I GTNNAC 3 cut(s) 608, 908, 1879
Hpy99I CGWCG 2 cut(s) 315, 1614
HpyAV CCTTC 4 cut(s) 1111, 1336, 1460, 1524
HpyCH4III ACNGT 2 cut(s) 466, 737
HpyCH4IV ACGT 5 cut(s) 886, 1579, 1609, 1843, 2116
HpyF10VI GCNNNNNNNGC 7 cut(s) 401, 566, 623, 632, 989, 1940, 2276
HpyF3I CTNAG 9 cut(s) 330, 397, 508, 1224, 1375, 1662, 1893, 2193, 2199
HpySE526I ACGT 5 cut(s) 886, 1579, 1609, 1843, 2116
HspAI GCGC 2 cut(s) 402, 865
Kpn2I TCCGGA 1 cut(s) 415
KpnI GGTACC 1 cut(s) 1485
Ksp22I TGATCA 1 cut(s) 217
Kzo9I GATC 7 cut(s) 43, 217, 265, 1033, 1953, 2128, 2164
LmnI GCTCC 5 cut(s) 901, 1054, 1591, 1789, 1875
Lsp1109I GCAGC 9 cut(s) 359, 463, 613, 616, 619, 790, 849, 1525, 1528
LweI GCATC 3 cut(s) 481, 979, 1360
MaeI CTAG 5 cut(s) 140, 158, 335, 614, 1241
MaeII ACGT 5 cut(s) 886, 1579, 1609, 1843, 2116
MaeIII GTNAC 3 cut(s) 1099, 1556, 2231
MalI GATC 7 cut(s) 45, 219, 267, 1035, 1955, 2130, 2166
MboI GATC 7 cut(s) 43, 217, 265, 1033, 1953, 2128, 2164
MflI RGATCY 2 cut(s) 1033, 2128
MhlI GDGCHC 1 cut(s) 1826
MlyI GAGTC 3 cut(s) 495, 632, 806
MmeI TCCRAC 2 cut(s) 421, 485
MroI TCCGGA 1 cut(s) 415
MslI CAYNNNNRTG 1 cut(s) 735
MspA1I CMGCKG 2 cut(s) 986, 1538
MspI CCGG 5 cut(s) 416, 751, 783, 995, 1488
MspR9I CCNGG 8 cut(s) 752, 808, 995, 1527, 1590, 1712, 1930, 2178
Mva1269I GAATGC 1 cut(s) 317
MvaI CCWGG 6 cut(s) 808, 1527, 1590, 1712, 1930, 2178
MvnI CGCG 1 cut(s) 310
MwoI GCNNNNNNNGC 7 cut(s) 401, 566, 623, 632, 989, 1940, 2276
NciI CCSGG 2 cut(s) 752, 995
NcoI CCATGG 1 cut(s) 380
NdeII GATC 7 cut(s) 43, 217, 265, 1033, 1953, 2128, 2164
NlaIV GGNNCC 4 cut(s) 676, 1483, 1749, 1871
NmeAIII GCCGAG 1 cut(s) 1882
NmuCI GTSAC 3 cut(s) 1099, 1556, 2231
NruI TCGCGA 1 cut(s) 310
NspI RCATGY 2 cut(s) 763, 1240
PagI TCATGA 1 cut(s) 1453
PctI GAATGC 1 cut(s) 317
PfeI GAWTC 7 cut(s) 13, 19, 151, 533, 1994, 2022, 2222
PflMI CCANNNNNTGG 2 cut(s) 856, 1526
PfoI TCCNGGA 3 cut(s) 1588, 1710, 2176
PinAI ACCGGT 1 cut(s) 1487
PleI GAGTC 3 cut(s) 495, 632, 806
PpsI GAGTC 3 cut(s) 495, 632, 806
PshBI ATTAAT 2 cut(s) 1250, 1917
PsiI TTATAA 1 cut(s) 428
Psp1406I AACGTT 1 cut(s) 1579
Psp6I CCWGG 6 cut(s) 806, 1525, 1588, 1710, 1928, 2176
PspGI CCWGG 6 cut(s) 806, 1525, 1588, 1710, 1928, 2176
PspN4I GGNNCC 4 cut(s) 676, 1483, 1749, 1871
PspPI GGNCC 2 cut(s) 674, 833
PstI CTGCAG 1 cut(s) 637
PstNI CAGNNNCTG 1 cut(s) 1541
PsuI RGATCY 2 cut(s) 1033, 2128
PvuII CAGCTG 1 cut(s) 1538
RruI TCGCGA 1 cut(s) 310
RsaI GTAC 7 cut(s) 685, 1259, 1467, 1483, 1673, 1846, 2139
RsaNI GTAC 7 cut(s) 684, 1258, 1466, 1482, 1672, 1845, 2138
RseI CAYNNNNRTG 1 cut(s) 735
Sau3AI GATC 7 cut(s) 43, 217, 265, 1033, 1953, 2128, 2164
Sau96I GGNCC 2 cut(s) 674, 833
ScaI AGTACT 1 cut(s) 685
SchI GAGTC 3 cut(s) 495, 632, 806
ScrFI CCNGG 8 cut(s) 752, 808, 995, 1527, 1590, 1712, 1930, 2178
SduI GDGCHC 1 cut(s) 1826
SfaNI GCATC 3 cut(s) 481, 979, 1360
SfcI CTRYAG 2 cut(s) 633, 2046
SinI GGWCC 1 cut(s) 833
SmiMI CAYNNNNRTG 1 cut(s) 735
SmlI CTYRAG 1 cut(s) 1174
SmoI CTYRAG 1 cut(s) 1174
SsiI CCGC 3 cut(s) 692, 931, 984
SspI AATATT 3 cut(s) 721, 938, 1777
SspMI CTAG 5 cut(s) 140, 158, 335, 614, 1241
StyD4I CCNGG 8 cut(s) 750, 806, 993, 1525, 1588, 1710, 1928, 2176
StyI CCWWGG 4 cut(s) 380, 1545, 1735, 2160
TaaI ACNGT 2 cut(s) 466, 737
TaiI ACGT 5 cut(s) 889, 1582, 1612, 1846, 2119
TaqI TCGA 5 cut(s) 11, 178, 1072, 1889, 1949
TatI WGTACW 1 cut(s) 683
TauI GCSGC 1 cut(s) 986
TfiI GAWTC 7 cut(s) 13, 19, 151, 533, 1994, 2022, 2222
TscAI CASTG 3 cut(s) 742, 1512, 2270
TseFI GTSAC 3 cut(s) 1099, 1556, 2231
TseI GCWGC 9 cut(s) 347, 476, 626, 629, 632, 803, 862, 1538, 1541
Tsp45I GTSAC 3 cut(s) 1099, 1556, 2231
TspDTI ATGAA 9 cut(s) 267, 816, 860, 1362, 1442, 1773, 1953, 2016, 2259
TspGWI ACGGA 2 cut(s) 1683, 1736
TspRI CASTG 3 cut(s) 742, 1512, 2270
Van91I CCANNNNNTGG 2 cut(s) 856, 1526
VpaK11BI GGWCC 1 cut(s) 833
VspI ATTAAT 2 cut(s) 1250, 1917
XagI CCTNNNNNAGG 1 cut(s) 1454
XapI RAATTY 4 cut(s) 543, 562, 822, 1898
XceI RCATGY 2 cut(s) 763, 1240
XspI CTAG 5 cut(s) 140, 158, 335, 614, 1241
ZrmI AGTACT 1 cut(s) 685
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.