RLG00000026826

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000598
Physical Location & Seq
Reverse (-)
6311393 .. 6316354
4962 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026826

Sequence Viewer

Length: 3261 bp
ATGGCGGAGGAAGGCGGGAAGAGCTTTGCCAGGAGAGACAGGCTTCTGGAGATTGAGACAATGGCTCGAAAATGGTGGGAGGAGAAACAAGTTTTCAGGGCAGAGTCTTGTGAGAAGCCTCCCGGACCAGGGGAGAAGTTCTTTGGAAACTTCCCCTTCCCTTATATGAATGGTTCTCTGCATCTCGGACATGCTTTTTCACTCTCGAAGCTGGAGTTTGCTGCGGCCTTTCATAGATTGAGAGGCGCAAATGTGCTCCTGCCTTTTGCTTTTCACTGTACTGGCATGCCCATCAAAGCCTCAGCTGATAAGCTTACTAGGGAGATTCAACAGTTTGGAGAACCTCCTGTTTTTCCCAAAAAGGAGGAGAAGGGGAATGAGGAAGTGGAAGTGGAAGCCGAGGATGTCAATGCAGGTGCACCTCCAGCCAAGCATAAGGGAAAGAAGTCGAAGGCTGCATCAAAATCAAGCGGCGAAGTGTACCAATGGGAAATTATGCGGAGCTTTGGCCTCTCAGACAGTGACATATCCAAATTTCAGGATCCTTATAAGTGGTTGACTTATTTTCCCCCATTGGCAGTGGAGGACCTCAAGGCTTTTGGCCTGGGCTGTGACTGGAGACGTTCCTTTATTACTACAGACATGAATCCTTTTTTCGACGCCTTTGTGAGGTGGCAGGTTAGGAAACTGAAATCCATGGGCAAGATTGTTAAAGATCTCCGTTACACGATATACTCTCCGTTGGATGGGCAACCGTGTGCAGATCATGATAGGTCCAGTGGTGAAGGAGTTCAGCCTCAAGAATACACTGTCATCAAAATGGAACTGATGGCACCTTTTCCTTCTAAATTGGGAGTGTTGGAGGGAAGGAAAGTGTTCCTTGCTGCAGCAACACTGAGACCTGAGACCATGTATGGACAAACTAATGCATGGGTATTGCCTGATGGTAAGTATGGTGCCTTTGAAATCAATGAAACAGATGTGTTTATTCTTACTCAGAGAGCAGCACGTAATCTTGCTTATCAAAAGTACTCTAGGGTCCCAGAGAAACCTACTTGCTTGGTTGAACTGACTGGTTATGATTTGATTGGCCTTCCATTGAAGTCGCCACTAGCACTGAATGAGATTATTTATGCTCTTCCTATGTTGACTGTCCTAACAGACAAAGGTACTGGGATTGTGACCAGTGTACCTACTGATTCTCCAGATGATTATATGGCCTTGCATGACTTGAAATCAAAACCTGCTTTTAGGGCAAAATATGGTGTGAAAGATGAGTGGGTACTACCCTTTGAGAATATCCCAATCATTGATATTCCAGGATTTGGAAATAATGCTGCGGAAAAGGTCTGTGCAGATCTCAAGATCAAAAGCCAGAATGAGAAAGAGAAGCTAGCAGAAGCCAAGAGGTTGACATACTTGAAAGGATTCAATGAAGGAACACTGATTGTGGGGGAGTTTAAGGGAAGGAAAGTCCAGGAGGTTAAACCCTTGATAAGGAGCAAGCTTATAGAGGCGGGGGAGGCAATATCATATAGTGAGCCTGAGAAGCGAGTACTATCAAGATCTGGTGATGAATGTGTTGTTGCGCTTACAGATCAATGGTACATCACATACGGGGAAACTGAATGGAAAAAATTGGCGGAAGAGTGCTTGTCCGGCATGAGTTTGTATTCTGATGAGACACGGCATGGATTTGAACACACATTGAGCTGGTTGAATCAGTGGGCTTGTTCACGTTCTTTTGGTCTTGGGACTCGCATCCCCTGGGATGAAGAATTCTTAGTTGAGTCATTGTCTGATTCAACTATTTACATGGCTTATTACACCATTGCTCACTTCCTACACAATGGAGACATGTATGGTTCCAGCAAATCTGCAATTAAACCTGAACAAATGACTGATGAGGTCTGGGATTTTGTTTTCTGTGATGGCCCATATCCTAAATCATCTGAGATCTCATCATCAATTCTGAGTAAGATGAAGCAGGAGTTTGAATATTGGTATCCTTTTGATCTTCGGGTGTCTGGCAAGGATCTAATACAAAATCATTTAACCTTCTGCATCTACAACCATACAGCTATTATGTCCAAGAAGCACTGGCCTCGTGGTTTCAGATGCAATGGGCACATAATGCTCAATGCTCAGAAAATGTCCAAGTCTACAGGGAACTTTAGGACAATTCGCCAGGCAATTAAGGAATTTTCTGCTGATGCCACTCGCTTTTCTTTGGCTGATGCTGGAGATGGTGTTGATGATGCAAATTTTGCATTTGACACTGCCAATGCTGCAATCCTGCGGCTCACTAAAGAGATCTCATGGACGGAAGAAGTTTTGGGTGCAGACTCTTCTTTGAGACCAGGCCCCCCAACTACTTATGCTGATAGGGTCTTTGCGAATGAGATAAATATTGCTGTGAACAGGACTGAGCAGAATTACAGTGGTTATATGTTCCGAGAAGCTCTCAAGACTGGATTTTATGATCTTCAAGCTGCTAGGGATGAGTATCGGTTTTCGTGTGGTGATGGTGGAATGAACCGTGATTTGTTGTGGCGTTTTATGGATGTGCAGACACGTCTTATTACTCCAATCTGCCCACACTATGGAGAATATGTATGGAGGGAACTCTTGAAGAAGGACGGGTTTGTGGTAAACGCAGGATGGCCTTTGGCTGATGCTCCGGACCTAACCCTCCAGAGTGCCAATAAGTATTTGCAGGACTCAATCATTTTAATGAGGAAGCTCCTCCAAAAGCAAGTTTTAGGCTCAAAGAAGGGTAATAAGAAGGGTACTCCAGTTACAGCGTTGACAGAAGACCACAATCTAACTGGCTTAATCTATGTGAATGAGCACTTTGATGGGTGGAAGGCAGAGTGCCTGAAAATCCTCCAAATTAACTTTAACAGGGAGGGTTGTACTTTTTCTTCAGACACGGTGATACAAGAAGCATTGCAAAAAAGTTCTATTGGTCAGTCGAAAGATTTTAGACAAATCCAAAAGCTTTGTATGCCTTTCATGAAATTCAAGAAGGATGAAGCAATTGCAATTGGGGCTCAGGCCTTGGATTTGAAGCTACCTTTTGGCGAGATTGAGGTCCTTCAAGAGAACTTGGACTTAATTAAGAGACAGCTTGGTCTTCAAGAGGTAGAAATATTGTCTGCTACAAATCCTGATGCACTTTCTAAAGCCGGTTCTCTTGTCAAACTGTTAGAGCAGAATCCTCCAGCACCCGGAAGCCCTACTGCCATCTTCTTGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

1087

Amino Acids

122.59

Weight (kDa)

6.16

Isoelectric Point (pI)

45.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1 PF00133 24 - 104 4.3e-08 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1 PF00133 193 - 755 1.3e-16 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g PF09334 662 - 761 2.5e-08 tRNA synthetases class I (M)
tRNA-synt_1e PF01406 669 - 745 3.2e-09 tRNA synthetases class I (C) catalytic domain
Anticodon_1 PF08264 795 - 924 1.5e-13 Anticodon-binding domain of tRNA ligase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000598)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09620
fragaria_vesca FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_3g34581 FvH4_4g33720 FvH4_4g33720
malus_domestica MD08G1244600.v1.1 MD15G1439400.v1.1
prunus_persica Prupe.1G582000_v2.0.a1 Prupe.2G015100_v2.0.a1 Prupe.2G123100_v2.0.a1
pyrus_communis pycom08g21210 pycom08g21220 pycom15g38730
rosa_chinensis RchiOBHm_Chr1g0336121 RchiOBHm_Chr1g0353811 RchiOBHm_Chr1g0373331 RchiOBHm_Chr4g0443191 RchiOBHm_Chr4g0443201 RchiOBHm_Chr4g0443211
rosa_laevigata RLG00000003122 RLG00000005948 RLG00000026826 RLG00000028251 RLG00000028252 RLG00000028253 RLG00000029377
rosa_multiflora Rmu_co8002498.1_g000001 Rmu_co8262279.1_g000001 Rmu_co8314139.1_g000001 Rmu_co8393711.1_g000001 Rmu_co8419167.1_g000001 Rmu_sc0007968.1_g000004 Rmu_sc0018919.1_g000004 Rmu_sc0027447.1_g000001
rosa_roxburghii Rroxscaffold_4G00284260 Rroxscaffold_4G00301490 Rroxscaffold_4G00315860 Rroxscaffold_5G00383790 Rroxscaffold_5G00383800
rosa_rugosa Rorug01G0123100 Rorug01G0238700 Rorug01G0377100 Rorug01G0377100 Rorug01G0377200 Rorug01G0377300 Rorug04G0344700 Rorug05G0347900.1
rosa_samantha Rh1AG143800 Rh1AG250300 Rh1AG386300 Rh1BG113400 Rh1BG220800 Rh1BG220900 Rh1BG350400 Rh1CG135500 Rh1CG233100 Rh1CG363500 Rh1DG150200 Rh1DG247700 Rh1DG247800 Rh1DG381300 Rh4AG399400 Rh4AG399500 Rh4BG411000 Rh4BG411100 Rh4BG411200 Rh4CG426500 Rh4CG426600 Rh4DG406100 Rh4DG406200 Rh4DG406300
rosa_wichuraiana Rw0G015190 Rw1G021850 Rw1G034870 Rw4G034440 Rw4G034450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 549
AarI CACCTGC 1 cut(s) 404
AasI GACNNNNNNGTC 1 cut(s) 3132
Acc36I ACCTGC 3 cut(s) 404, 667, 1252
AccB1I GGYRCC 2 cut(s) 832, 956
AccB7I CCANNNNNTGG 2 cut(s) 1325, 2603
AccI GTMKAC 1 cut(s) 2162
AccIII TCCGGA 1 cut(s) 2680
AciI CCGC 9 cut(s) 5, 15, 224, 471, 499, 1340, 1517, 1643, 2299
AclWI GGATC 3 cut(s) 536, 549, 2043
AcsI RAATTY 5 cut(s) 533, 1778, 2201, 2263, 3020
AcuI CTGAAG 1 cut(s) 2910
AcyI GRCGYC 1 cut(s) 660
AfiI CCNNNNNNNGG 8 cut(s) 128, 129, 669, 746, 1325, 1497, 2603, 3230
AflIII ACRYGT 2 cut(s) 1857, 2573
AjiI CACGTC 1 cut(s) 2576
AjnI CCWGG 8 cut(s) 29, 127, 603, 1318, 1476, 1766, 2187, 2359
Alw21I GWGCWC 3 cut(s) 258, 421, 2853
Alw26I GTCTC 9 cut(s) 30, 50, 613, 892, 899, 1676, 1848, 2350, 3118
Alw44I GTGCAC 1 cut(s) 417
AlwI GGATC 3 cut(s) 536, 549, 2043
Aor13HI TCCGGA 1 cut(s) 2680
ApaLI GTGCAC 1 cut(s) 417
ApeKI GCWGC 8 cut(s) 221, 455, 884, 887, 1004, 1337, 2288, 2492
ApoI RAATTY 5 cut(s) 533, 1778, 2201, 2263, 3020
Asp700I GAANNNNTTC 3 cut(s) 789, 875, 1427
AspLEI GCGC 2 cut(s) 248, 1591
AspS9I GGNCC 8 cut(s) 125, 586, 774, 1039, 1934, 2363, 2683, 3094
AsuC2I CCSGG 2 cut(s) 123, 3231
AsuHPI GGTGA 4 cut(s) 794, 1583, 2534, 2947
AsuNHI GCTAGC 1 cut(s) 1393
AvaII GGWCC 6 cut(s) 125, 586, 774, 1039, 2683, 3094
BaeGI GKGCMC 2 cut(s) 421, 2130
BaeI ACNNNNGTAYC 4 cut(s) 1173, 1206, 2781, 2814
BamHI GGATCC 1 cut(s) 541
BanI GGYRCC 2 cut(s) 832, 956
BanII GRGCYC 1 cut(s) 3055
BarI GAAGNNNNNNTAC 2 cut(s) 2908, 2940
BauI CACGAG 1 cut(s) 2106
BbsI GAAGAC 2 cut(s) 2820, 3128
Bbv12I GWGCWC 3 cut(s) 258, 421, 2853
BbvCI CCTCAGC 1 cut(s) 301
BbvI GCAGC 8 cut(s) 208, 442, 871, 899, 1016, 1324, 2275, 2479
BceAI ACGGC 1 cut(s) 1703
BcgI CGANNNNNNTGC 2 cut(s) 2087, 2121
BciT130I CCWGG 8 cut(s) 31, 129, 605, 1320, 1478, 1768, 2189, 2361
BciVI GTATCC 1 cut(s) 2016
BcnI CCSGG 2 cut(s) 123, 3231
BcoDI GTCTC 9 cut(s) 30, 50, 613, 892, 899, 1676, 1848, 2350, 3118
BfaI CTAG 5 cut(s) 318, 1035, 1112, 1394, 2496
BfmI CTRYAG 3 cut(s) 636, 885, 2163
BfuAI ACCTGC 3 cut(s) 404, 667, 1252
BfuI GTATCC 1 cut(s) 2016
BglII AGATCT 5 cut(s) 715, 1357, 1565, 1956, 2313
BmcAI AGTACT 2 cut(s) 1031, 1557
Bme18I GGWCC 6 cut(s) 125, 586, 774, 1039, 2683, 3094
BmgBI CACGTC 1 cut(s) 2576
BmgT120I GGNCC 8 cut(s) 125, 586, 774, 1039, 1934, 2363, 2683, 3094
BmiI GGNNCC 7 cut(s) 543, 834, 958, 1040, 1041, 1867, 2365
BmrI ACTGGG 1 cut(s) 1182
BmtI GCTAGC 1 cut(s) 1397
BmuI ACTGGG 1 cut(s) 1182
BpiI GAAGAC 2 cut(s) 2820, 3128
BplI GAGNNNNNCTC 2 cut(s) 2448, 2480
BpmI CTGGAG 9 cut(s) 68, 233, 408, 637, 1188, 2262, 2678, 2778, 3207
Bpu10I CCTNAGC 2 cut(s) 301, 3054
BpuEI CTTGAG 4 cut(s) 575, 783, 1346, 2450
BpuMI CCSGG 2 cut(s) 123, 3231
BsaAI YACGTR 1 cut(s) 1010
BsaBI GATNNNNATC 1 cut(s) 2505
BsaHI GRCGYC 1 cut(s) 660
BsaI GGTCTC 3 cut(s) 892, 899, 2350
BsaJI CCNNGG 7 cut(s) 128, 399, 604, 696, 1766, 1767, 3060
BsaWI WCCGGW 1 cut(s) 2680
BsaXI ACNNNNNCTCC 6 cut(s) 1186, 1216, 2235, 2265, 2598, 2628
Bsc4I CCNNNNNNNGG 8 cut(s) 128, 129, 669, 746, 1325, 1497, 2603, 3230
Bse118I RCCGGY 1 cut(s) 3188
Bse3DI GCAATG 3 cut(s) 1830, 2128, 2948
Bse8I GATNNNNATC 1 cut(s) 2505
BseAI TCCGGA 1 cut(s) 2680
BseBI CCWGG 8 cut(s) 31, 129, 605, 1320, 1478, 1768, 2189, 2361
BseDI CCNNGG 7 cut(s) 128, 399, 604, 696, 1766, 1767, 3060
BseGI GGATG 8 cut(s) 409, 751, 1761, 1777, 2506, 2569, 2666, 3037
BseJI GATNNNNATC 1 cut(s) 2505
BseLI CCNNNNNNNGG 8 cut(s) 128, 129, 669, 746, 1325, 1497, 2603, 3230
BseMI GCAATG 3 cut(s) 1830, 2128, 2948
BseRI GAGGAG 3 cut(s) 95, 380, 2735
BseSI GKGCMC 2 cut(s) 421, 2130
BseXI GCAGC 8 cut(s) 208, 442, 871, 899, 1016, 1324, 2275, 2479
BsgI GTGCAG 4 cut(s) 780, 1374, 2361, 2588
BshNI GGYRCC 2 cut(s) 832, 956
BsiHKAI GWGCWC 3 cut(s) 258, 421, 2853
BsiSI CCGG 5 cut(s) 123, 1659, 2681, 3189, 3231
BslFI GGGAC 2 cut(s) 1025, 1768
BslI CCNNNNNNNGG 8 cut(s) 128, 129, 669, 746, 1325, 1497, 2603, 3230
BsmAI GTCTC 9 cut(s) 30, 50, 613, 892, 899, 1676, 1848, 2350, 3118
BsmBI CGTCTC 1 cut(s) 613
BsmFI GGGAC 2 cut(s) 1025, 1768
Bso31I GGTCTC 3 cut(s) 892, 899, 2350
Bsp1286I GDGCHC 5 cut(s) 258, 421, 2130, 2853, 3055
Bsp13I TCCGGA 1 cut(s) 2680
Bsp19I CCATGG 1 cut(s) 696
BspACI CCGC 9 cut(s) 5, 15, 224, 471, 499, 1340, 1517, 1643, 2299
BspEI TCCGGA 1 cut(s) 2680
BspHI TCATGA 2 cut(s) 766, 3015
BspLI GGNNCC 7 cut(s) 543, 834, 958, 1040, 1041, 1867, 2365
BspMAI CTGCAG 1 cut(s) 889
BspMI ACCTGC 3 cut(s) 404, 667, 1252
BspOI GCTAGC 1 cut(s) 1397
BspPI GGATC 3 cut(s) 536, 549, 2043
BspQI GCTCTTC 2 cut(s) 14, 1143
BspT107I GGYRCC 2 cut(s) 832, 956
BspTNI GGTCTC 3 cut(s) 892, 899, 2350
BsrDI GCAATG 3 cut(s) 1830, 2128, 2948
BsrFI RCCGGY 1 cut(s) 3188
BssAI RCCGGY 1 cut(s) 3188
BssECI CCNNGG 7 cut(s) 128, 399, 604, 696, 1766, 1767, 3060
BssNI GRCGYC 1 cut(s) 660
BssSI CACGAG 1 cut(s) 2106
BssT1I CCWWGG 2 cut(s) 696, 3060
Bst2BI CACGAG 1 cut(s) 2106
Bst2UI CCWGG 8 cut(s) 31, 129, 605, 1320, 1478, 1768, 2189, 2361
Bst6I CTCTTC 4 cut(s) 14, 1143, 1641, 2353
BstACI GRCGYC 1 cut(s) 660
BstAPI GCANNNNNTGC 2 cut(s) 2134, 2267
BstBAI YACGTR 1 cut(s) 1010
BstC8I GCNNGC 3 cut(s) 287, 1395, 1505
BstDSI CCRYGG 1 cut(s) 696
BstENI CCTNNNNNAGG 2 cut(s) 667, 1495
BstF5I GGATG 8 cut(s) 409, 751, 1761, 1777, 2506, 2569, 2666, 3037
BstHHI GCGC 2 cut(s) 248, 1591
BstMAI GTCTC 9 cut(s) 30, 50, 613, 892, 899, 1676, 1848, 2350, 3118
BstNI CCWGG 8 cut(s) 31, 129, 605, 1320, 1478, 1768, 2189, 2361
BstNSI RCATGY 3 cut(s) 194, 289, 1861
BstSFI CTRYAG 3 cut(s) 636, 885, 2163
BstSLI GKGCMC 2 cut(s) 421, 2130
BstV1I GCAGC 8 cut(s) 208, 442, 871, 899, 1016, 1324, 2275, 2479
BstV2I GAAGAC 2 cut(s) 2820, 3128
BstX2I RGATCY 7 cut(s) 541, 715, 1357, 1565, 1956, 2035, 2313
BstYI RGATCY 7 cut(s) 541, 715, 1357, 1565, 1956, 2035, 2313
BsuI GTATCC 1 cut(s) 2016
BtgI CCRYGG 1 cut(s) 696
BtrI CACGTC 1 cut(s) 2576
BtsCI GGATG 8 cut(s) 409, 751, 1761, 1777, 2506, 2569, 2666, 3037
BtsI GCAGTG 2 cut(s) 585, 2277
BveI ACCTGC 3 cut(s) 404, 667, 1252
Cac8I GCNNGC 3 cut(s) 287, 1395, 1505
CciI TCATGA 2 cut(s) 766, 3015
CfoI GCGC 2 cut(s) 248, 1591
Cfr10I RCCGGY 1 cut(s) 3188
Cfr13I GGNCC 8 cut(s) 125, 586, 774, 1039, 1934, 2363, 2683, 3094
CseI GACGC 1 cut(s) 668
DrdI GACNNNNNNGTC 1 cut(s) 3132
DseDI GACNNNNNNGTC 1 cut(s) 3132
Eam1104I CTCTTC 4 cut(s) 14, 1143, 1641, 2353
EarI CTCTTC 4 cut(s) 14, 1143, 1641, 2353
EciI GGCGGA 2 cut(s) 20, 1658
Eco130I CCWWGG 2 cut(s) 696, 3060
Eco147I AGGCCT 1 cut(s) 3059
Eco24I GRGCYC 1 cut(s) 3055
Eco31I GGTCTC 3 cut(s) 892, 899, 2350
Eco47I GGWCC 6 cut(s) 125, 586, 774, 1039, 2683, 3094
Eco57I CTGAAG 1 cut(s) 2910
EcoNI CCTNNNNNAGG 2 cut(s) 667, 1495
EcoO109I RGGNCCY 4 cut(s) 586, 1039, 2363, 3094
EcoRI GAATTC 1 cut(s) 1778
EcoRII CCWGG 8 cut(s) 29, 127, 603, 1318, 1476, 1766, 2187, 2359
EcoT14I CCWWGG 2 cut(s) 696, 3060
EcoT22I ATGCAT 1 cut(s) 931
EcoT38I GRGCYC 1 cut(s) 3055
ErhI CCWWGG 2 cut(s) 696, 3060
Esp3I CGTCTC 1 cut(s) 613
FalI AAGNNNNNCTT 2 cut(s) 864, 896
FaqI GGGAC 2 cut(s) 1025, 1768
FauI CCCGC 2 cut(s) 8, 1510
FblI GTMKAC 1 cut(s) 2162
FokI GGATG 8 cut(s) 416, 758, 1748, 1784, 2513, 2576, 2673, 3044
FriOI GRGCYC 1 cut(s) 3055
FspBI CTAG 5 cut(s) 318, 1035, 1112, 1394, 2496
GlaI GCGC 2 cut(s) 247, 1590
GsuI CTGGAG 9 cut(s) 68, 233, 408, 637, 1188, 2262, 2678, 2778, 3207
HapII CCGG 5 cut(s) 123, 1659, 2681, 3189, 3231
HgaI GACGC 1 cut(s) 668
HhaI GCGC 2 cut(s) 248, 1591
Hin1I GRCGYC 1 cut(s) 660
Hin6I GCGC 2 cut(s) 246, 1589
HinP1I GCGC 2 cut(s) 246, 1589
HincII GTYRAC 4 cut(s) 558, 1149, 1413, 2808
HindII GTYRAC 4 cut(s) 558, 1149, 1413, 2808
HindIII AAGCTT 3 cut(s) 311, 1505, 2999
HpaII CCGG 5 cut(s) 123, 1659, 2681, 3189, 3231
HphI GGTGA 4 cut(s) 794, 1583, 2534, 2947
Hpy99I CGWCG 1 cut(s) 662
HpyCH4IV ACGT 4 cut(s) 622, 1009, 1738, 2575
HpySE526I ACGT 4 cut(s) 622, 1009, 1738, 2575
Hsp92I GRCGYC 1 cut(s) 660
HspAI GCGC 2 cut(s) 246, 1589
KflI GGGWCCC 1 cut(s) 1039
Kpn2I TCCGGA 1 cut(s) 2680
LguI GCTCTTC 2 cut(s) 14, 1143
LmnI GCTCC 5 cut(s) 261, 501, 1500, 2683, 2748
Lsp1109I GCAGC 8 cut(s) 208, 442, 871, 899, 1016, 1324, 2275, 2479
MaeI CTAG 5 cut(s) 318, 1035, 1112, 1394, 2496
MaeII ACGT 4 cut(s) 622, 1009, 1738, 2575
MaeIII GTNAC 5 cut(s) 521, 611, 722, 1180, 2797
MfeI CAATTG 2 cut(s) 3039, 3045
MflI RGATCY 7 cut(s) 541, 715, 1357, 1565, 1956, 2035, 2313
MhlI GDGCHC 5 cut(s) 258, 421, 2130, 2853, 3055
MlyI GAGTC 5 cut(s) 113, 1750, 1799, 2339, 2714
MmeI TCCRAC 2 cut(s) 723, 840
Mph1103I ATGCAT 1 cut(s) 931
MroI TCCGGA 1 cut(s) 2680
MroXI GAANNNNTTC 3 cut(s) 789, 875, 1427
MslI CAYNNNNRTG 3 cut(s) 818, 2732, 2856
MspA1I CMGCKG 1 cut(s) 305
MspI CCGG 5 cut(s) 123, 1659, 2681, 3189, 3231
MunI CAATTG 2 cut(s) 3039, 3045
MvaI CCWGG 8 cut(s) 31, 129, 605, 1320, 1478, 1768, 2189, 2361
NciI CCSGG 2 cut(s) 123, 3231
NcoI CCATGG 1 cut(s) 696
NheI GCTAGC 1 cut(s) 1393
NlaIV GGNNCC 7 cut(s) 543, 834, 958, 1040, 1041, 1867, 2365
NmeAIII GCCGAG 1 cut(s) 424
NmuCI GTSAC 3 cut(s) 521, 611, 1180
NsiI ATGCAT 1 cut(s) 931
NspI RCATGY 3 cut(s) 194, 289, 1861
PacI TTAATTAA 1 cut(s) 3120
PaeI GCATGC 1 cut(s) 289
PagI TCATGA 2 cut(s) 766, 3015
PaqCI CACCTGC 1 cut(s) 404
PasI CCCWGGG 1 cut(s) 1767
PceI AGGCCT 1 cut(s) 3059
PciI ACATGT 1 cut(s) 1857
PciSI GCTCTTC 2 cut(s) 14, 1143
PdmI GAANNNNTTC 3 cut(s) 789, 875, 1427
PfeI GAWTC 7 cut(s) 325, 646, 1199, 1428, 1720, 1802, 3217
PflMI CCANNNNNTGG 2 cut(s) 1325, 2603
PfoI TCCNGGA 3 cut(s) 121, 1318, 1476
PleI GAGTC 5 cut(s) 112, 1750, 1798, 2339, 2714
PpsI GAGTC 5 cut(s) 112, 1750, 1798, 2339, 2714
Ppu21I YACGTR 1 cut(s) 1010
PpuMI RGGWCCY 3 cut(s) 586, 1039, 3094
PscI ACATGT 1 cut(s) 1857
PsiI TTATAA 1 cut(s) 549
Psp5II RGGWCCY 3 cut(s) 586, 1039, 3094
Psp6I CCWGG 8 cut(s) 29, 127, 603, 1318, 1476, 1766, 2187, 2359
PspGI CCWGG 8 cut(s) 29, 127, 603, 1318, 1476, 1766, 2187, 2359
PspN4I GGNNCC 7 cut(s) 543, 834, 958, 1040, 1041, 1867, 2365
PspPI GGNCC 8 cut(s) 125, 586, 774, 1039, 1934, 2363, 2683, 3094
PspPPI RGGWCCY 3 cut(s) 586, 1039, 3094
PstI CTGCAG 1 cut(s) 889
PsuI RGATCY 7 cut(s) 541, 715, 1357, 1565, 1956, 2035, 2313
PvuII CAGCTG 1 cut(s) 305
RseI CAYNNNNRTG 3 cut(s) 818, 2732, 2856
SapI GCTCTTC 2 cut(s) 14, 1143
Sau96I GGNCC 8 cut(s) 125, 586, 774, 1039, 1934, 2363, 2683, 3094
ScaI AGTACT 2 cut(s) 1031, 1557
SchI GAGTC 5 cut(s) 113, 1750, 1799, 2339, 2714
SduI GDGCHC 5 cut(s) 258, 421, 2130, 2853, 3055
SfcI CTRYAG 3 cut(s) 636, 885, 2163
SinI GGWCC 6 cut(s) 125, 586, 774, 1039, 2683, 3094
SmiMI CAYNNNNRTG 3 cut(s) 818, 2732, 2856
SmlI CTYRAG 4 cut(s) 590, 798, 1361, 2465
SmoI CTYRAG 4 cut(s) 590, 798, 1361, 2465
SphI GCATGC 1 cut(s) 289
SseBI AGGCCT 1 cut(s) 3059
SsiI CCGC 9 cut(s) 5, 15, 224, 471, 499, 1340, 1517, 1643, 2299
SspI AATATT 3 cut(s) 2000, 2410, 3153
SspMI CTAG 5 cut(s) 318, 1035, 1112, 1394, 2496
StuI AGGCCT 1 cut(s) 3059
StyI CCWWGG 2 cut(s) 696, 3060
TaiI ACGT 4 cut(s) 625, 1012, 1741, 2578
TaqI TCGA 5 cut(s) 67, 206, 449, 657, 2975
TatI WGTACW 4 cut(s) 278, 1029, 1555, 2915
TauI GCSGC 3 cut(s) 227, 474, 2302
TfiI GAWTC 7 cut(s) 325, 646, 1199, 1428, 1720, 1802, 3217
TseFI GTSAC 3 cut(s) 521, 611, 1180
TseI GCWGC 8 cut(s) 221, 455, 884, 887, 1004, 1337, 2288, 2492
Tsp45I GTSAC 3 cut(s) 521, 611, 1180
TspGWI ACGGA 3 cut(s) 710, 729, 2339
Van91I CCANNNNNTGG 2 cut(s) 1325, 2603
VneI GTGCAC 1 cut(s) 417
VpaK11BI GGWCC 6 cut(s) 125, 586, 774, 1039, 2683, 3094
XagI CCTNNNNNAGG 2 cut(s) 667, 1495
XapI RAATTY 5 cut(s) 533, 1778, 2201, 2263, 3020
XceI RCATGY 3 cut(s) 194, 289, 1861
XcmI CCANNNNNNNNNTGG 1 cut(s) 2825
XmiI GTMKAC 1 cut(s) 2162
XmnI GAANNNNTTC 3 cut(s) 789, 875, 1427
XspI CTAG 5 cut(s) 318, 1035, 1112, 1394, 2496
ZrmI AGTACT 2 cut(s) 1031, 1557
Zsp2I ATGCAT 1 cut(s) 931
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.