RLG00000029377

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000598
Physical Location & Seq
Reverse (-)
37419876 .. 37431375
11500 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029377

Sequence Viewer

Length: 3954 bp
ATGAAGCGGCAAGAACAGAGGCCTCTCAGCCTTGATGAAGCTTTATCATCTTCTTATGACGATGCTTCTGGTCCTAGGTCAGAAGCCAGAGTTGGGTTAAGTTACGTGCTTGGTCAAGTTATGTGCTTGTCAGCATATATCCAAGCTAGAACATTTTTCGCCTCAGCCGAAAAGTTCTTGAAACCGAAGCCTATTAGCCCTCGTCGCAGTTCTCTTCTGTTCTTCAAATTCGGTTACACTAGTGCAGTGCTGCTGCTAATGAGAGTAAAGTGGACGACAAAGCTCCACTGTCGCAGTTTGTTTAAAAGGTACTTTAGTAGTGGTACTGAGATGGCGGAGGAAGGCGGGAAGAGCTTTGCCAGGAGAGACCATCTCCTAGAGATTGAGGCAAAGGCTCGAAGCTGGTGGGAAGACAAACAAGTTTTCAGGGCCCAGTCTCGTGCAGAGCCGCCCGGCCCAGGGGAGAAGTTCTTTGGAAACTTCCCCTTCCCTTATATGAACGGTTATCTGCATCTCGGACATGCCTTCTCACTGTCGAAGCTGGAGTTTGCTGCAGCCTATCATAGATTGAGAGGCGCAAATGTCCTCTTGCCATTTGCTTTTCACTGTACTGGCATGCCCATCAAAGCCTCTGCCGATAAGCTTATTAGGGAGATTCAACAGTTTGGAGAACCTCCTGTTTTTCCCAAAGAAGTAGAGAAAGGGGACGAGGAAGTGGAAGTGGAAGCAGAGGATGTCAATGCAGGTGAACCTCCAGCCAAGCATAAGGGAAAGAAGTCAAAGGCTGCATCAAAATCAAGCGGCCAAGTGTACCAATGGGAAATTATGCAGAGCTTTGGCCTCTCCAACAGTGACATATCCCAATTTCAGGACCCTTATATGTGGTTGACTTATTTTCCCCCATTGGCTGTGGAGGACCTCAAGGCTTTTGGCCTGGGCTGTGACTGGAGACGTTCCTTTATTACTACAGACATGAACCCTTTTTTCGACGCCTTTGTGAGGTGGCAGGTTAGGAAACTGAAATCCATGGGTAAGATTGTCAAAGATCTCCGTTACACGATATACTCTCCGATGGATGGGCAACCCTGTGCAGATCATGATAGGGCCAGTGGTGAAGGAGTTCAGCCCCAAGAATATACTGTCATCAAAATGGAACTGATGGCACCTTTTCCTTCTAAATTGGGAGTGTTGGAGGGGAGGAAAGTGTTCCTTGCTGCAGCAACACTGAGACCTGAGACCATGTATGGACAAACAAATGCATGGGTATTGCCTGATGGTAAGTATGGTGCTTATGAAATCAATGAAACAGATGTGTTCATTCTTACACAGAGAGCAGCACGTAATCTTGCTTATCAAAAGTACTCTAGGGTCCCAGAGAAACCTACTTGCTTGGTTGAACTGACTGGTTATGATTTGATTGGCCTTCCATTGAAGTCGCCACTAGCAGTGAATCAGATTATTTATGCTCTTCCTATGTTGACTGTCCTAACAGACAAAGGTACTGGGATTGTGACCAGTGTACCTACTGATTCTCCGGATGACTATATGGCCTTGCATGACTTGAAATCAAAACCTGCTTTTAGGGCAAAATTTGGTGTGAAAGATGAGTGGGTACTGCCCTTTGAGATTATCCCAATCATCGATATTCCAGGATATGGAAATAAGGCTGCGGAAAAGGTCTGTGCAGATCTCAAGATCAAAAGCCAGAATGAGAAAGAGAAGCTAGCAGAAGCCAAGAGGTTGACATACTTGAAAGGATTCAATGAAGGAACACTGATTGTGGGGGAATTTACGGGAAGGAAAGTCCAGGAGGTTAAACCCTTGATAAGGAGCAAGCTTATAGAGGCGGGGGAGGCAATATCATACAGTGAACCTGAGAAGCGGGTACTATCACGATCAGGTGATGAATGTGTTGTGGCGCTTACAGATCAATGGTACATCACTTACGGGGAAACTGAATGGAAAAAACTGGCTGAGGAGTGCTTGTCCGGCATGAATTTGTATTCTGATGAGACACGGCATGGATTTGAACACACATTGAGCTGGTTGAATCAGTGGGCTTGTTCACGATCTTTTGGTCTTGGGACTCGCATCCCCTGGGATGAAGAATTCTTAGTTGAGTCATTGTCTGATTCAACTATTTACATGGCTTATTACACCATTGCTCACTTCCTACACAATGGAGACATGTATGGTTCCAGCAAATCTGCAATTAAACCTGAACAAATGACTGATGAGGTCTGGGATTTTATTTTCTGTGATGGCCCATATCCTAAATCATCCGACATCTCATCATCAATTCTGAGTGAGATGAAGCAGGAGTTTGAATATTGGTATCCTTTTGATCTTCGGGTGTCTGGCAAGGATCTAATACAAAATCATTTAACCTTCTGCATCTACAACCATACAGCTATCATGTCCAAGAAGCACTGGCCTCGTGGTTTCAGATGCAATGGGCACATAATGCTCAATGCTCAGAAAATGTCCAAGTCCACAGGAAACTTTAGGACAATTCGCCAGGCAATTGACGAATTTTCTGCTGATGCCACTCGCTTTTCTTTGGCTGATGCTGGAGATGGTGTTGATGATGCTAATTTTGCATTTGACACTGCCAATGCTGCAATCCTGCGGCTTACTAAAGAGATCTCATGGATGGAAGAAGTTTTAGGTGCAAACTCTTCTTTGAGACCAGGACCCCCAACTACTTATGCTGACAGGGTCTTTGCGAATGAGATAAATATTGCTGTGAACAGGACTGAGCAGAATTACAGCGATTACATGTTCCGAGAAGCCCTCAAGACTGGATTTTATGATCTTCAGGCTGCTAGGGATGAGTATCGGTTTTCATGTGGTGCTGGGGGAATGAATCGTGATTTGGTGTGGCGTTTTATGGATGTGCAGACACGTCTTATTACTCCAATCTGCCCACACTACGGAGAATACGTATGGAAGGAACTTTTGAAGAAGGATGGCTTTGTGGTAAACGCAGGATGGCCTTTGGCTGACGCTCCGGACCTAACCCTCCAGAGTGCCAATAAGTATTTGCAGGACTCAATCATTTCAATGAGGAAGCTCCTCCAAAAGCAAGTTTCAAGCTCAAAGAAGGGTAAGAAGGGTACTCCAGTCACAGTGTTGACAGAAGACAATAATCTGACCGGCTTAATCTATGTGAACGAGCACTTTGATGGATGGAAGGCAGAGTGCCTGAAAATCCTCCAAATTAACTTTAACAGAGACAGTCGTATTTTTTCTTCAGACAAGGTGATACAAGAAGAGTTGCAAAAAAGTTCTATTGGTCAGTCGAAAGATTTTAGACAAATCCAGAAGCTTTGTATGCCTTTCATGAAGTTCAAGAAGGATGAGGCAATTGCAATTGGGGCTCAGGCCTTGGATTTGAAGCTACCTTTTGGCGAGATTGAGGTCCTTCAAGAGAACTTGGACTTAATTAAGAGACAACTTGGTCTTCAAGAGGTAGAAATATTGTCTGCTACAAATCCTGATGCACTTTCTAAAGCCGGTTCTCTTGTCAAACTGTTAGAGCAGAATCCTCCAGCACCCGGAAGCCCTACTGCCATCTTCTTGACCTGGGTTTTGAGTTTAGAGAGTGACGGCGACGGCTTGCTCCTCATCGTCTGTCTAGCAGAGGCGCGATCTATCATTGTCGAGCGAGTTTCACAGGTGAGGCAGGGGAACGGCGGTGAGCGAGGATTGACTGACCAGAAGGCCTCTGTTGTTGCGGTGGTGGTTGGTTGTAAGATCGGAGATCCTAATCGGCCTCTCAACTTTGGCCGAGGAGGTGGCGCTGATGCTGCGTTGGAGGGGACGTCCGCCGGAATCTATGGCGGCAGCGGCGATGATTGGCAGAGGCAGTTTCCAGTCTGCTATGGTGTTGTTTTTGGTACCCTTGGGCCTGGGCTGGGGACAAAGTCTTTTGGGCTTGGTCTCAATCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

1318

Amino Acids

147.66

Weight (kDa)

6.42

Isoelectric Point (pI)

41.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1 PF00133 134 - 216 5.4e-08 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1 PF00133 305 - 865 1.2e-16 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g PF09334 773 - 871 2.7e-08 tRNA synthetases class I (M)
tRNA-synt_1e PF01406 779 - 855 1.7e-08 tRNA synthetases class I (C) catalytic domain
Anticodon_1 PF08264 905 - 1029 5.7e-13 Anticodon-binding domain of tRNA ligase
RBD_LARS1 PF24810 1058 - 1134 2.8e-06 Leucine--tRNA ligase, RagD-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000598)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09620
fragaria_vesca FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_3g34581 FvH4_4g33720 FvH4_4g33720
malus_domestica MD08G1244600.v1.1 MD15G1439400.v1.1
prunus_persica Prupe.1G582000_v2.0.a1 Prupe.2G015100_v2.0.a1 Prupe.2G123100_v2.0.a1
pyrus_communis pycom08g21210 pycom08g21220 pycom15g38730
rosa_chinensis RchiOBHm_Chr1g0336121 RchiOBHm_Chr1g0353811 RchiOBHm_Chr1g0373331 RchiOBHm_Chr4g0443191 RchiOBHm_Chr4g0443201 RchiOBHm_Chr4g0443211
rosa_laevigata RLG00000003122 RLG00000005948 RLG00000026826 RLG00000028251 RLG00000028252 RLG00000028253 RLG00000029377
rosa_multiflora Rmu_co8002498.1_g000001 Rmu_co8262279.1_g000001 Rmu_co8314139.1_g000001 Rmu_co8393711.1_g000001 Rmu_co8419167.1_g000001 Rmu_sc0007968.1_g000004 Rmu_sc0018919.1_g000004 Rmu_sc0027447.1_g000001
rosa_roxburghii Rroxscaffold_4G00284260 Rroxscaffold_4G00301490 Rroxscaffold_4G00315860 Rroxscaffold_5G00383790 Rroxscaffold_5G00383800
rosa_rugosa Rorug01G0123100 Rorug01G0238700 Rorug01G0377100 Rorug01G0377100 Rorug01G0377200 Rorug01G0377300 Rorug04G0344700 Rorug05G0347900.1
rosa_samantha Rh1AG143800 Rh1AG250300 Rh1AG386300 Rh1BG113400 Rh1BG220800 Rh1BG220900 Rh1BG350400 Rh1CG135500 Rh1CG233100 Rh1CG363500 Rh1DG150200 Rh1DG247700 Rh1DG247800 Rh1DG381300 Rh4AG399400 Rh4AG399500 Rh4BG411000 Rh4BG411100 Rh4BG411200 Rh4CG426500 Rh4CG426600 Rh4DG406100 Rh4DG406200 Rh4DG406300
rosa_wichuraiana Rw0G015190 Rw1G021850 Rw1G034870 Rw4G034440 Rw4G034450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 734
AasI GACNNNNNNGTC 1 cut(s) 3459
AatII GACGTC 1 cut(s) 3827
Acc36I ACCTGC 3 cut(s) 734, 997, 1582
Acc65I GGTACC 1 cut(s) 3899
AccB1I GGYRCC 2 cut(s) 1162, 3899
AccB7I CCANNNNNTGG 1 cut(s) 1655
AccII CGCG 1 cut(s) 3649
AccIII TCCGGA 2 cut(s) 1534, 3010
AclWI GGATC 2 cut(s) 2373, 3758
AcoI YGGCCR 2 cut(s) 802, 3787
AcsI RAATTY 6 cut(s) 227, 1589, 1787, 1996, 2108, 2531
AcuI CTGAAG 2 cut(s) 2801, 3237
AcyI GRCGYC 2 cut(s) 990, 3824
AflIII ACRYGT 3 cut(s) 2187, 2778, 2903
AhlI ACTAGT 1 cut(s) 239
AjiI CACGTC 1 cut(s) 2906
AjuI GAANNNNNNNTTGG 4 cut(s) 75, 107, 2858, 2890
Alw21I GWGCWC 1 cut(s) 3180
AlwI GGATC 2 cut(s) 2373, 3758
Aor13HI TCCGGA 2 cut(s) 1534, 3010
ApaI GGGCCC 1 cut(s) 433
ApoI RAATTY 6 cut(s) 227, 1589, 1787, 1996, 2108, 2531
ArsI GACNNNNNNTTYG 4 cut(s) 2705, 2737, 3574, 3606
Asp700I GAANNNNTTC 3 cut(s) 1119, 1205, 1757
Asp718I GGTACC 1 cut(s) 3899
AspA2I CCTAGG 1 cut(s) 74
AspLEI GCGC 4 cut(s) 578, 1921, 3649, 3803
AsuC2I CCSGG 2 cut(s) 453, 3558
AsuHPI GGTGA 6 cut(s) 758, 1124, 1913, 3274, 3691, 3710
AsuNHI GCTAGC 1 cut(s) 1723
AvaII GGWCC 7 cut(s) 71, 871, 916, 1369, 2693, 3013, 3421
AvrII CCTAGG 1 cut(s) 74
BaeGI GKGCMC 2 cut(s) 433, 2460
BaeI ACNNNNGTAYC 6 cut(s) 301, 334, 1503, 1536, 3108, 3141
BanI GGYRCC 2 cut(s) 1162, 3899
BanII GRGCYC 2 cut(s) 433, 3382
BarI GAAGNNNNNNTAC 2 cut(s) 3235, 3267
BauI CACGAG 2 cut(s) 438, 2436
BbsI GAAGAC 3 cut(s) 417, 3147, 3455
Bbv12I GWGCWC 1 cut(s) 3180
BbvCI CCTCAGC 2 cut(s) 163, 1974
BceAI ACGGC 4 cut(s) 2033, 3625, 3631, 3709
BcgI CGANNNNNNTGC 4 cut(s) 2417, 2451, 2519, 2553
BciVI GTATCC 1 cut(s) 2346
BcnI CCSGG 2 cut(s) 453, 3558
BcuI ACTAGT 1 cut(s) 239
BfmI CTRYAG 3 cut(s) 552, 966, 1215
BfoI RGCGCY 2 cut(s) 1922, 3804
BfuAI ACCTGC 3 cut(s) 734, 997, 1582
BfuI GTATCC 1 cut(s) 2346
BglII AGATCT 3 cut(s) 1045, 1687, 2643
BlnI CCTAGG 1 cut(s) 74
BmcAI AGTACT 1 cut(s) 1361
Bme18I GGWCC 7 cut(s) 71, 871, 916, 1369, 2693, 3013, 3421
BmgBI CACGTC 1 cut(s) 2906
BmiI GGNNCC 8 cut(s) 431, 873, 1164, 1370, 1371, 2197, 2695, 3901
BmrI ACTGGG 2 cut(s) 427, 1512
BmtI GCTAGC 1 cut(s) 1727
BmuI ACTGGG 2 cut(s) 427, 1512
BpiI GAAGAC 3 cut(s) 417, 3147, 3455
BplI GAGNNNNNCTC 4 cut(s) 357, 389, 2778, 2810
BpmI CTGGAG 7 cut(s) 563, 738, 967, 2592, 3008, 3105, 3534
Bpu10I CCTNAGC 3 cut(s) 163, 1974, 3381
BpuEI CTTGAG 3 cut(s) 905, 1676, 2780
BpuMI CCSGG 2 cut(s) 453, 3558
Bsa29I ATCGAT 1 cut(s) 1641
BsaAI YACGTR 3 cut(s) 106, 1340, 2944
BsaBI GATNNNNATC 2 cut(s) 2835, 3768
BsaHI GRCGYC 2 cut(s) 990, 3824
BsaI GGTCTC 5 cut(s) 360, 1222, 1229, 2680, 3947
BsaWI WCCGGW 2 cut(s) 1534, 3010
Bse118I RCCGGY 2 cut(s) 3155, 3515
Bse3DI GCAATG 2 cut(s) 2160, 2458
Bse8I GATNNNNATC 2 cut(s) 2835, 3768
BseAI TCCGGA 2 cut(s) 1534, 3010
BseCI ATCGAT 1 cut(s) 1641
BseJI GATNNNNATC 2 cut(s) 2835, 3768
BseMI GCAATG 2 cut(s) 2160, 2458
BseRI GAGGAG 4 cut(s) 1991, 3065, 3614, 3807
BseSI GKGCMC 2 cut(s) 433, 2460
BseYI CCCAGC 2 cut(s) 2855, 3916
BsgI GTGCAG 5 cut(s) 264, 462, 1110, 1704, 2918
Bsh1236I CGCG 1 cut(s) 3649
BshNI GGYRCC 2 cut(s) 1162, 3899
BshVI ATCGAT 1 cut(s) 1641
BsiHKAI GWGCWC 1 cut(s) 3180
BsiSI CCGG 8 cut(s) 453, 1535, 1989, 3011, 3156, 3516, 3558, 3831
BslFI GGGAC 5 cut(s) 719, 1355, 2098, 3835, 3934
BsmBI CGTCTC 1 cut(s) 943
BsmFI GGGAC 5 cut(s) 719, 1355, 2098, 3835, 3934
Bso31I GGTCTC 5 cut(s) 360, 1222, 1229, 2680, 3947
Bsp120I GGGCCC 1 cut(s) 429
Bsp1286I GDGCHC 4 cut(s) 433, 2460, 3180, 3382
Bsp13I TCCGGA 2 cut(s) 1534, 3010
Bsp19I CCATGG 1 cut(s) 1026
BspDI ATCGAT 1 cut(s) 1641
BspEI TCCGGA 2 cut(s) 1534, 3010
BspFNI CGCG 1 cut(s) 3649
BspHI TCATGA 2 cut(s) 1096, 3342
BspLI GGNNCC 8 cut(s) 431, 873, 1164, 1370, 1371, 2197, 2695, 3901
BspMAI CTGCAG 2 cut(s) 556, 1219
BspMI ACCTGC 3 cut(s) 734, 997, 1582
BspOI GCTAGC 1 cut(s) 1727
BspPI GGATC 2 cut(s) 2373, 3758
BspQI GCTCTTC 2 cut(s) 344, 1473
BspT107I GGYRCC 2 cut(s) 1162, 3899
BspTNI GGTCTC 5 cut(s) 360, 1222, 1229, 2680, 3947
BsrDI GCAATG 2 cut(s) 2160, 2458
BsrFI RCCGGY 2 cut(s) 3155, 3515
BssAI RCCGGY 2 cut(s) 3155, 3515
BssNI GRCGYC 2 cut(s) 990, 3824
BssSI CACGAG 2 cut(s) 438, 2436
BssT1I CCWWGG 4 cut(s) 74, 1026, 3387, 3904
Bst2BI CACGAG 2 cut(s) 438, 2436
Bst6I CTCTTC 5 cut(s) 219, 344, 1473, 2683, 3267
BstACI GRCGYC 2 cut(s) 990, 3824
BstAPI GCANNNNNTGC 1 cut(s) 2464
BstBAI YACGTR 3 cut(s) 106, 1340, 2944
BstC8I GCNNGC 4 cut(s) 617, 1725, 1835, 3620
BstDSI CCRYGG 1 cut(s) 1026
BstENI CCTNNNNNAGG 2 cut(s) 997, 1825
BstFNI CGCG 1 cut(s) 3649
BstH2I RGCGCY 2 cut(s) 1922, 3804
BstHHI GCGC 4 cut(s) 578, 1921, 3649, 3803
BstNSI RCATGY 4 cut(s) 524, 619, 2191, 2782
BstSFI CTRYAG 3 cut(s) 552, 966, 1215
BstSLI GKGCMC 2 cut(s) 433, 2460
BstSNI TACGTA 1 cut(s) 2944
BstUI CGCG 1 cut(s) 3649
BstV2I GAAGAC 3 cut(s) 417, 3147, 3455
BstX2I RGATCY 5 cut(s) 1045, 1687, 2365, 2643, 3763
BstYI RGATCY 5 cut(s) 1045, 1687, 2365, 2643, 3763
Bsu15I ATCGAT 1 cut(s) 1641
BsuI GTATCC 1 cut(s) 2346
BsuTUI ATCGAT 1 cut(s) 1641
BtgI CCRYGG 1 cut(s) 1026
BtgZI GCGATG 1 cut(s) 3867
BtrI CACGTC 1 cut(s) 2906
BtsI GCAGTG 3 cut(s) 252, 1452, 2607
BveI ACCTGC 3 cut(s) 734, 997, 1582
Cac8I GCNNGC 4 cut(s) 617, 1725, 1835, 3620
CciI TCATGA 2 cut(s) 1096, 3342
CfoI GCGC 4 cut(s) 578, 1921, 3649, 3803
Cfr10I RCCGGY 2 cut(s) 3155, 3515
ClaI ATCGAT 1 cut(s) 1641
CseI GACGC 2 cut(s) 998, 3014
DraI TTTAAA 1 cut(s) 304
DrdI GACNNNNNNGTC 1 cut(s) 3459
DseDI GACNNNNNNGTC 1 cut(s) 3459
EaeI YGGCCR 2 cut(s) 802, 3787
Eam1104I CTCTTC 5 cut(s) 219, 344, 1473, 2683, 3267
EarI CTCTTC 5 cut(s) 219, 344, 1473, 2683, 3267
EciI GGCGGA 2 cut(s) 350, 3817
Eco105I TACGTA 1 cut(s) 2944
Eco130I CCWWGG 4 cut(s) 74, 1026, 3387, 3904
Eco147I AGGCCT 3 cut(s) 22, 3386, 3725
Eco24I GRGCYC 2 cut(s) 433, 3382
Eco31I GGTCTC 5 cut(s) 360, 1222, 1229, 2680, 3947
Eco47I GGWCC 7 cut(s) 71, 871, 916, 1369, 2693, 3013, 3421
Eco57I CTGAAG 2 cut(s) 2801, 3237
EcoNI CCTNNNNNAGG 2 cut(s) 997, 1825
EcoO109I RGGNCCY 6 cut(s) 429, 871, 916, 1369, 2693, 3421
EcoRI GAATTC 1 cut(s) 2108
EcoT14I CCWWGG 4 cut(s) 74, 1026, 3387, 3904
EcoT22I ATGCAT 1 cut(s) 1261
EcoT38I GRGCYC 2 cut(s) 433, 3382
ErhI CCWWGG 4 cut(s) 74, 1026, 3387, 3904
Esp3I CGTCTC 1 cut(s) 943
FalI AAGNNNNNCTT 4 cut(s) 1194, 1226, 2957, 2989
FaqI GGGAC 5 cut(s) 719, 1355, 2098, 3835, 3934
FauI CCCGC 3 cut(s) 338, 1840, 1875
FriOI GRGCYC 2 cut(s) 433, 3382
GlaI GCGC 4 cut(s) 577, 1920, 3648, 3802
GsaI CCCAGC 2 cut(s) 2859, 3920
GsuI CTGGAG 7 cut(s) 563, 738, 967, 2592, 3008, 3105, 3534
HaeII RGCGCY 2 cut(s) 1922, 3804
HapII CCGG 8 cut(s) 453, 1535, 1989, 3011, 3156, 3516, 3558, 3831
HgaI GACGC 2 cut(s) 998, 3014
HhaI GCGC 4 cut(s) 578, 1921, 3649, 3803
Hin1I GRCGYC 2 cut(s) 990, 3824
Hin6I GCGC 4 cut(s) 576, 1919, 3647, 3801
HinP1I GCGC 4 cut(s) 576, 1919, 3647, 3801
HincII GTYRAC 4 cut(s) 888, 1479, 1743, 3135
HindII GTYRAC 4 cut(s) 888, 1479, 1743, 3135
HindIII AAGCTT 4 cut(s) 39, 641, 1835, 3326
HpaII CCGG 8 cut(s) 453, 1535, 1989, 3011, 3156, 3516, 3558, 3831
HphI GGTGA 6 cut(s) 758, 1124, 1913, 3274, 3691, 3710
Hpy99I CGWCG 3 cut(s) 207, 992, 3617
HpyCH4IV ACGT 6 cut(s) 105, 952, 1339, 2905, 2943, 3824
HpySE526I ACGT 6 cut(s) 105, 952, 1339, 2905, 2943, 3824
Hsp92I GRCGYC 2 cut(s) 990, 3824
HspAI GCGC 4 cut(s) 576, 1919, 3647, 3801
KflI GGGWCCC 1 cut(s) 1369
Kpn2I TCCGGA 2 cut(s) 1534, 3010
KpnI GGTACC 1 cut(s) 3903
LguI GCTCTTC 2 cut(s) 344, 1473
LmnI GCTCC 5 cut(s) 288, 1830, 3013, 3078, 3627
MaeII ACGT 6 cut(s) 105, 952, 1339, 2905, 2943, 3824
MaeIII GTNAC 8 cut(s) 101, 233, 851, 941, 1052, 1510, 3124, 3605
MfeI CAATTG 3 cut(s) 2523, 3366, 3372
MflI RGATCY 5 cut(s) 1045, 1687, 2365, 2643, 3763
MhlI GDGCHC 4 cut(s) 433, 2460, 3180, 3382
MlyI GAGTC 3 cut(s) 2080, 2129, 3044
MmeI TCCRAC 4 cut(s) 870, 1170, 2307, 3795
Mph1103I ATGCAT 1 cut(s) 1261
MroI TCCGGA 2 cut(s) 1534, 3010
MroXI GAANNNNTTC 3 cut(s) 1119, 1205, 1757
MslI CAYNNNNRTG 3 cut(s) 1148, 3062, 3183
MspA1I CMGCKG 1 cut(s) 3849
MspI CCGG 8 cut(s) 453, 1535, 1989, 3011, 3156, 3516, 3558, 3831
MunI CAATTG 3 cut(s) 2523, 3366, 3372
MvnI CGCG 1 cut(s) 3649
NciI CCSGG 2 cut(s) 453, 3558
NcoI CCATGG 1 cut(s) 1026
NheI GCTAGC 1 cut(s) 1723
NlaIV GGNNCC 8 cut(s) 431, 873, 1164, 1370, 1371, 2197, 2695, 3901
NmeAIII GCCGAG 1 cut(s) 3815
NmuCI GTSAC 5 cut(s) 851, 941, 1510, 3124, 3605
NsiI ATGCAT 1 cut(s) 1261
NspI RCATGY 4 cut(s) 524, 619, 2191, 2782
PacI TTAATTAA 1 cut(s) 3447
PaeI GCATGC 1 cut(s) 619
PagI TCATGA 2 cut(s) 1096, 3342
PaqCI CACCTGC 1 cut(s) 734
PasI CCCWGGG 2 cut(s) 458, 2097
PceI AGGCCT 3 cut(s) 22, 3386, 3725
PciI ACATGT 2 cut(s) 2187, 2778
PciSI GCTCTTC 2 cut(s) 344, 1473
PcsI WCGNNNNNNNCGW 2 cut(s) 165, 2940
PdmI GAANNNNTTC 3 cut(s) 1119, 1205, 1757
PfeI GAWTC 9 cut(s) 655, 1450, 1529, 1758, 2050, 2132, 2866, 3544, 3834
PflFI GACNNNGTC 2 cut(s) 2717, 3925
PflMI CCANNNNNTGG 1 cut(s) 1655
PfoI TCCNGGA 2 cut(s) 1648, 1806
PleI GAGTC 3 cut(s) 2080, 2128, 3044
PpsI GAGTC 3 cut(s) 2080, 2128, 3044
Ppu21I YACGTR 3 cut(s) 106, 1340, 2944
PpuMI RGGWCCY 5 cut(s) 871, 916, 1369, 2693, 3421
PscI ACATGT 2 cut(s) 2187, 2778
Psp5II RGGWCCY 5 cut(s) 871, 916, 1369, 2693, 3421
PspFI CCCAGC 2 cut(s) 2855, 3916
PspN4I GGNNCC 8 cut(s) 431, 873, 1164, 1370, 1371, 2197, 2695, 3901
PspOMI GGGCCC 1 cut(s) 429
PspPPI RGGWCCY 5 cut(s) 871, 916, 1369, 2693, 3421
PstI CTGCAG 2 cut(s) 556, 1219
PsuI RGATCY 5 cut(s) 1045, 1687, 2365, 2643, 3763
PsyI GACNNNGTC 2 cut(s) 2717, 3925
RseI CAYNNNNRTG 3 cut(s) 1148, 3062, 3183
SapI GCTCTTC 2 cut(s) 344, 1473
ScaI AGTACT 1 cut(s) 1361
SchI GAGTC 3 cut(s) 2080, 2129, 3044
SduI GDGCHC 4 cut(s) 433, 2460, 3180, 3382
SfcI CTRYAG 3 cut(s) 552, 966, 1215
SinI GGWCC 7 cut(s) 71, 871, 916, 1369, 2693, 3013, 3421
SmiMI CAYNNNNRTG 3 cut(s) 1148, 3062, 3183
SmlI CTYRAG 3 cut(s) 920, 1691, 2795
SmoI CTYRAG 3 cut(s) 920, 1691, 2795
SnaBI TACGTA 1 cut(s) 2944
SpeI ACTAGT 1 cut(s) 239
SphI GCATGC 1 cut(s) 619
SseBI AGGCCT 3 cut(s) 22, 3386, 3725
SspI AATATT 3 cut(s) 2330, 2740, 3480
StuI AGGCCT 3 cut(s) 22, 3386, 3725
StyI CCWWGG 4 cut(s) 74, 1026, 3387, 3904
TaiI ACGT 6 cut(s) 108, 955, 1342, 2908, 2946, 3827
TaqI TCGA 6 cut(s) 397, 536, 987, 1641, 3302, 3663
TatI WGTACW 2 cut(s) 608, 1359
TauI GCSGC 6 cut(s) 10, 451, 804, 2632, 3846, 3852
TfiI GAWTC 9 cut(s) 655, 1450, 1529, 1758, 2050, 2132, 2866, 3544, 3834
TseFI GTSAC 5 cut(s) 851, 941, 1510, 3124, 3605
Tsp45I GTSAC 5 cut(s) 851, 941, 1510, 3124, 3605
TspGWI ACGGA 2 cut(s) 1040, 2949
Tth111I GACNNNGTC 2 cut(s) 2717, 3925
Van91I CCANNNNNTGG 1 cut(s) 1655
VpaK11BI GGWCC 7 cut(s) 71, 871, 916, 1369, 2693, 3013, 3421
XagI CCTNNNNNAGG 2 cut(s) 997, 1825
XapI RAATTY 6 cut(s) 227, 1589, 1787, 1996, 2108, 2531
XceI RCATGY 4 cut(s) 524, 619, 2191, 2782
XmaJI CCTAGG 1 cut(s) 74
XmnI GAANNNNTTC 3 cut(s) 1119, 1205, 1757
ZraI GACGTC 1 cut(s) 3825
ZrmI AGTACT 1 cut(s) 1361
Zsp2I ATGCAT 1 cut(s) 1261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.