Rh4CG426600

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000598
Physical Location & Seq
Reverse (-)
66914224 .. 66914889
666 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG426600.1

Sequence Viewer

Length: 666 bp
ATGGCAGTCGAAGGTGGGAAGAAATCTAGGAGAGATCGTCTCAAAGAGATTGAGGAGAAGGCACAAAAGTTGTGGGAAGAAAATGGTGTTTTCAGGGCTGAATCTTGTGAAAAGCCACCTGAACCAGGGGAGAAGTTCTTTGGGAACTTCCCTCTTCCTTACATGAATGGTTTTTTGCATGTAGGTCATGCATTCTCACTCTCGAAGCTAGAGTTTTCTGCTCGTTTTCATAGGTTAAGAGGTGCTAATGTGCTGCTCCCTGTTGGTTTCCACCTCACTGGCATGCCCATCAAAAGTATTGGCCTGTCTGCCATTGATATATCAGAACTCCAGAATCCATCGAACTGGTTGAGCTGCTTCCCTCGAGTTGCAGTGAAAGAGCTCAAGGCATTTGGCTTGGGCTGTGACTGGAGGCGCTCCTTTATTACCACAGACATGAACCCATACTTTGACAAGTTTGTGAGATGGCAGATGAGGAAACTAAAATCTATGGGTAAGATTGTGAAGGATGTCCGTTACACAATCTACTCTCCTTTGGATGGCCAGCCATGTGCAGATCATGTTAGGGAAACTGGTGAAGGAGTTCGGCCCCAAGAGTACACCATCATCAAAATGGAGGTGGTGGCACCTTTTCCTCCTAAACTGGGAGTGTTGAAGGGAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

221

Amino Acids

25.19

Weight (kDa)

9.49

Isoelectric Point (pI)

43.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1 PF00133 22 - 178 8.6e-12 tRNA synthetases class I (I, L, M and V)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000598)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09620
fragaria_vesca FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_3g34581 FvH4_4g33720 FvH4_4g33720
malus_domestica MD08G1244600.v1.1 MD15G1439400.v1.1
prunus_persica Prupe.1G582000_v2.0.a1 Prupe.2G015100_v2.0.a1 Prupe.2G123100_v2.0.a1
pyrus_communis pycom08g21210 pycom08g21220 pycom15g38730
rosa_chinensis RchiOBHm_Chr1g0336121 RchiOBHm_Chr1g0353811 RchiOBHm_Chr1g0373331 RchiOBHm_Chr4g0443191 RchiOBHm_Chr4g0443201 RchiOBHm_Chr4g0443211
rosa_laevigata RLG00000003122 RLG00000005948 RLG00000026826 RLG00000028251 RLG00000028252 RLG00000028253 RLG00000029377
rosa_multiflora Rmu_co8002498.1_g000001 Rmu_co8262279.1_g000001 Rmu_co8314139.1_g000001 Rmu_co8393711.1_g000001 Rmu_co8419167.1_g000001 Rmu_sc0007968.1_g000004 Rmu_sc0018919.1_g000004 Rmu_sc0027447.1_g000001
rosa_roxburghii Rroxscaffold_4G00284260 Rroxscaffold_4G00301490 Rroxscaffold_4G00315860 Rroxscaffold_5G00383790 Rroxscaffold_5G00383800
rosa_rugosa Rorug01G0123100 Rorug01G0238700 Rorug01G0377100 Rorug01G0377100 Rorug01G0377200 Rorug01G0377300 Rorug04G0344700 Rorug05G0347900.1
rosa_samantha Rh1AG143800 Rh1AG250300 Rh1AG386300 Rh1BG113400 Rh1BG220800 Rh1BG220900 Rh1BG350400 Rh1CG135500 Rh1CG233100 Rh1CG363500 Rh1DG150200 Rh1DG247700 Rh1DG247800 Rh1DG381300 Rh4AG399400 Rh4AG399500 Rh4BG411000 Rh4BG411100 Rh4BG411200 Rh4CG426500 Rh4CG426600 Rh4DG406100 Rh4DG406200 Rh4DG406300
rosa_wichuraiana Rw0G015190 Rw1G021850 Rw1G034870 Rw4G034440 Rw4G034450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 625
AcoI YGGCCR 1 cut(s) 541
AfaI GTAC 1 cut(s) 599
AfiI CCNNNNNNNGG 3 cut(s) 125, 539, 644
AgsI TTSAA 1 cut(s) 655
AjnI CCWGG 1 cut(s) 124
AluBI AGCT 3 cut(s) 208, 354, 382
AluI AGCT 3 cut(s) 208, 354, 382
Alw21I GWGCWC 1 cut(s) 384
Alw26I GTCTC 1 cut(s) 44
Ama87I CYCGRG 1 cut(s) 363
AoxI GGCC 3 cut(s) 301, 541, 587
ApeKI GCWGC 2 cut(s) 253, 354
Asp700I GAANNNNTTC 1 cut(s) 582
AspLEI GCGC 1 cut(s) 417
AspS9I GGNCC 1 cut(s) 588
AsuHPI GGTGA 1 cut(s) 587
AvaI CYCGRG 1 cut(s) 363
BalI TGGCCA 1 cut(s) 543
BanI GGYRCC 1 cut(s) 625
BanII GRGCYC 1 cut(s) 384
Bbv12I GWGCWC 1 cut(s) 384
BbvI GCAGC 2 cut(s) 240, 341
BccI CCATC 5 cut(s) 296, 346, 459, 533, 611
BciT130I CCWGG 1 cut(s) 126
BcoDI GTCTC 1 cut(s) 44
BfaI CTAG 2 cut(s) 27, 209
BfoI RGCGCY 1 cut(s) 418
BisI GCNGC 2 cut(s) 254, 355
BlsI GCNGC 2 cut(s) 255, 356
Bme1390I CCNGG 1 cut(s) 126
BmeT110I CYCGRG 1 cut(s) 363
BmgT120I GGNCC 1 cut(s) 588
BmiI GGNNCC 2 cut(s) 590, 627
BmrFI CCNGG 1 cut(s) 126
BmrI ACTGGG 1 cut(s) 653
BmuI ACTGGG 1 cut(s) 653
BplI GAGNNNNNCTC 2 cut(s) 24, 56
BpmI CTGGAG 2 cut(s) 314, 430
BpuEI CTTGAG 1 cut(s) 368
BsaJI CCNNGG 1 cut(s) 125
BsaXI ACNNNNNCTCC 2 cut(s) 22, 52
Bsc4I CCNNNNNNNGG 3 cut(s) 125, 539, 644
Bse1I ACTGG 5 cut(s) 283, 350, 413, 577, 648
BseBI CCWGG 1 cut(s) 126
BseDI CCNNGG 1 cut(s) 125
BseGI GGATG 2 cut(s) 514, 544
BseLI CCNNNNNNNGG 3 cut(s) 125, 539, 644
BseNI ACTGG 5 cut(s) 283, 350, 413, 577, 648
BseRI GAGGAG 1 cut(s) 68
BseXI GCAGC 2 cut(s) 240, 341
BsgI GTGCAG 1 cut(s) 573
BshFI GGCC 3 cut(s) 303, 543, 589
BshNI GGYRCC 1 cut(s) 625
BsiHKAI GWGCWC 1 cut(s) 384
BsiHKCI CYCGRG 1 cut(s) 363
BslI CCNNNNNNNGG 3 cut(s) 125, 539, 644
BsmAI GTCTC 1 cut(s) 44
BsmBI CGTCTC 1 cut(s) 44
BsmI GAATGC 1 cut(s) 191
BsnI GGCC 3 cut(s) 303, 543, 589
BsoBI CYCGRG 1 cut(s) 363
Bsp1286I GDGCHC 1 cut(s) 384
Bsp143I GATC 2 cut(s) 34, 556
BspANI GGCC 3 cut(s) 303, 543, 589
BspLI GGNNCC 2 cut(s) 590, 627
BspT107I GGYRCC 1 cut(s) 625
BsrI ACTGG 5 cut(s) 283, 350, 413, 577, 648
BssECI CCNNGG 1 cut(s) 125
BssMI GATC 2 cut(s) 34, 556
Bst2UI CCWGG 1 cut(s) 126
Bst6I CTCTTC 1 cut(s) 159
BstC8I GCNNGC 2 cut(s) 284, 545
BstENI CCTNNNNNAGG 1 cut(s) 123
BstF5I GGATG 2 cut(s) 514, 544
BstH2I RGCGCY 1 cut(s) 418
BstHHI GCGC 1 cut(s) 417
BstKTI GATC 2 cut(s) 37, 559
BstMAI GTCTC 1 cut(s) 44
BstMBI GATC 2 cut(s) 34, 556
BstNI CCWGG 1 cut(s) 126
BstNSI RCATGY 2 cut(s) 182, 286
BstSCI CCNGG 1 cut(s) 124
BstV1I GCAGC 2 cut(s) 240, 341
BstXI CCANNNNNNTGG 2 cut(s) 278, 345
BsuRI GGCC 3 cut(s) 303, 543, 589
BtsCI GGATG 2 cut(s) 514, 544
BtsI GCAGTG 1 cut(s) 378
BtsIMutI CAGTG 2 cut(s) 276, 378
Cac8I GCNNGC 2 cut(s) 284, 545
CfoI GCGC 1 cut(s) 417
Cfr13I GGNCC 1 cut(s) 588
Csp6I GTAC 1 cut(s) 598
CspCI CAANNNNNGTGG 2 cut(s) 53, 88
CviAII CATG 7 cut(s) 163, 179, 188, 283, 436, 549, 560
CviQI GTAC 1 cut(s) 598
DpnI GATC 2 cut(s) 36, 558
DpnII GATC 2 cut(s) 34, 556
EaeI YGGCCR 1 cut(s) 541
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
Ecl136II GAGCTC 1 cut(s) 382
Eco24I GRGCYC 1 cut(s) 384
Eco53kI GAGCTC 1 cut(s) 382
Eco88I CYCGRG 1 cut(s) 363
EcoICRI GAGCTC 1 cut(s) 382
EcoNI CCTNNNNNAGG 1 cut(s) 123
EcoRII CCWGG 1 cut(s) 124
EcoT22I ATGCAT 1 cut(s) 193
EcoT38I GRGCYC 1 cut(s) 384
Esp3I CGTCTC 1 cut(s) 44
FaeI CATG 7 cut(s) 166, 182, 191, 286, 439, 552, 563
FatI CATG 7 cut(s) 162, 178, 187, 282, 435, 548, 559
Fnu4HI GCNGC 2 cut(s) 254, 355
FokI GGATG 2 cut(s) 521, 551
FriOI GRGCYC 1 cut(s) 384
Fsp4HI GCNGC 2 cut(s) 254, 355
FspBI CTAG 2 cut(s) 27, 209
GlaI GCGC 1 cut(s) 416
GluI GCNGC 2 cut(s) 254, 355
GsuI CTGGAG 2 cut(s) 314, 430
HaeII RGCGCY 1 cut(s) 418
HaeIII GGCC 3 cut(s) 303, 543, 589
HhaI GCGC 1 cut(s) 417
Hin1II CATG 7 cut(s) 166, 182, 191, 286, 439, 552, 563
Hin6I GCGC 1 cut(s) 415
HinP1I GCGC 1 cut(s) 415
HinfI GANTC 2 cut(s) 101, 334
HphI GGTGA 1 cut(s) 587
Hpy166II GTNNAC 1 cut(s) 600
Hpy188I TCNGA 1 cut(s) 325
Hpy188III TCNNGA 2 cut(s) 202, 331
Hpy8I GTNNAC 1 cut(s) 600
HpyAV CCTTC 5 cut(s) 5, 52, 499, 572, 649
HpyCH4V TGCA 4 cut(s) 178, 191, 371, 554
Hsp92II CATG 7 cut(s) 166, 182, 191, 286, 439, 552, 563
HspAI GCGC 1 cut(s) 415
Kzo9I GATC 2 cut(s) 34, 556
LmnI GCTCC 2 cut(s) 261, 422
Lsp1109I GCAGC 2 cut(s) 240, 341
MaeI CTAG 2 cut(s) 27, 209
MaeIII GTNAC 2 cut(s) 404, 515
MalI GATC 2 cut(s) 36, 558
MboI GATC 2 cut(s) 34, 556
MboII GAAGA 3 cut(s) 31, 89, 146
MhlI GDGCHC 1 cut(s) 384
MlsI TGGCCA 1 cut(s) 543
MluNI TGGCCA 1 cut(s) 543
MnlI CCTC 9 cut(s) 46, 162, 233, 284, 372, 405, 468, 610, 645
Mox20I TGGCCA 1 cut(s) 543
Mph1103I ATGCAT 1 cut(s) 193
MroXI GAANNNNTTC 1 cut(s) 582
MscI TGGCCA 1 cut(s) 543
MseI TTAA 1 cut(s) 236
MslI CAYNNNNRTG 3 cut(s) 281, 434, 611
Msp20I TGGCCA 1 cut(s) 543
MspR9I CCNGG 1 cut(s) 126
Mva1269I GAATGC 1 cut(s) 191
MvaI CCWGG 1 cut(s) 126
NdeII GATC 2 cut(s) 34, 556
NlaIII CATG 7 cut(s) 166, 182, 191, 286, 439, 552, 563
NlaIV GGNNCC 2 cut(s) 590, 627
NmuCI GTSAC 1 cut(s) 404
NsiI ATGCAT 1 cut(s) 193
NspI RCATGY 2 cut(s) 182, 286
PaeI GCATGC 1 cut(s) 286
PaeR7I CTCGAG 1 cut(s) 363
PctI GAATGC 1 cut(s) 191
PdmI GAANNNNTTC 1 cut(s) 582
PfeI GAWTC 2 cut(s) 101, 334
PkrI GCNGC 2 cut(s) 255, 356
Psp124BI GAGCTC 1 cut(s) 384
Psp6I CCWGG 1 cut(s) 124
PspGI CCWGG 1 cut(s) 124
PspN4I GGNNCC 2 cut(s) 590, 627
PspPI GGNCC 1 cut(s) 588
PspXI VCTCGAGB 1 cut(s) 363
RsaI GTAC 1 cut(s) 599
RsaNI GTAC 1 cut(s) 598
RseI CAYNNNNRTG 3 cut(s) 281, 434, 611
SacI GAGCTC 1 cut(s) 384
SaqAI TTAA 1 cut(s) 236
SatI GCNGC 2 cut(s) 254, 355
Sau3AI GATC 2 cut(s) 34, 556
Sau96I GGNCC 1 cut(s) 588
ScrFI CCNGG 1 cut(s) 126
SduI GDGCHC 1 cut(s) 384
Sfr274I CTCGAG 1 cut(s) 363
SlaI CTCGAG 1 cut(s) 363
SmiMI CAYNNNNRTG 3 cut(s) 281, 434, 611
SmlI CTYRAG 2 cut(s) 363, 383
SmoI CTYRAG 2 cut(s) 363, 383
SphI GCATGC 1 cut(s) 286
SspMI CTAG 2 cut(s) 27, 209
SstI GAGCTC 1 cut(s) 384
StyD4I CCNGG 1 cut(s) 124
TaqI TCGA 4 cut(s) 9, 203, 341, 364
TatI WGTACW 1 cut(s) 597
TfiI GAWTC 2 cut(s) 101, 334
Tru1I TTAA 1 cut(s) 236
Tru9I TTAA 1 cut(s) 236
TscAI CASTG 2 cut(s) 283, 378
TseFI GTSAC 1 cut(s) 404
TseI GCWGC 2 cut(s) 253, 354
Tsp45I GTSAC 1 cut(s) 404
TspDTI ATGAA 3 cut(s) 179, 218, 452
TspGWI ACGGA 1 cut(s) 503
TspRI CASTG 2 cut(s) 283, 378
XagI CCTNNNNNAGG 1 cut(s) 123
XceI RCATGY 2 cut(s) 182, 286
XcmI CCANNNNNNNNNTGG 1 cut(s) 610
XhoI CTCGAG 1 cut(s) 363
XmnI GAANNNNTTC 1 cut(s) 582
XspI CTAG 2 cut(s) 27, 209
Zsp2I ATGCAT 1 cut(s) 193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.