FvH4_2g10290

O-acyltransferase (WSD1-like)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000634
Physical Location & Seq
Forward (+)
9122272 .. 9124842
2571 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g10290.t1

Sequence Viewer

Length: 1398 bp
ATGGAGTTTGAAGAAGAAGGACTGCAGCCAGTGAGCCCTCTCGGTCAGTACTTCGCTAACCGTGTGTTATCGGTATCAAATCTCGGTGTTTTGGAAATTGAGGTTCCAATCCATGAGCTTCCAGTTATGTCGTTGATTACAAATGTGTTCCTCCCCATCAGCCCTCGCTTCTCCTCTATGATGGTTGAGACACGGGGAAAGAAGCAATGGAAAAGGGTTGAAGTGGAGCTTGAAGACCATGTTTACATCCCGATTTTCCCTTCCGGCTTATCGCTGGCATCATACGATCGGTATCTTGATGACTATTTATCAAAGCTAGCAGTTGAAAGACTTCCAGAACACAGGCCTCTGTGGGAGATTCACATCATCAAGTACCCAACTACCAGTGCAGCCGGTACTATAATATTCAAGCTTCACCAAGCCCTTGGTGATGGCTTCTCTCTCATGGGTGCTCTTCTTTCCTGTCTACAAAGAGCTGAGAATCCTTCCCTTCCTCTAACCTTCCCGTCAAGAAAGAGGTCATCGCAACCGAGGAATAAGAATGGAAACTTTGTCACTAAAGTTTTCTCGTCCGTCTACAACACCATATCGGATATTTTAAACAGCAACATGGAGGAAGATGATCAAACACCAATCAGATCGGGGAATGATGGAATTGAGTTCAAGCCAATTGCAGTATCGACTATGACGATTTCTCTTGATCGACTCAAACTAATCAAGAGCAGGCTCGGAGTGACGATAAATGATGTTCTTACCGCGATGATCTTCCTGGGGACTCGACTATACATGCAAGAGATTAACAAAAGTTCCAGTGAAGCAAGGGGGACAGCAGTTGTTTTGCTCAACACAAGGATGCTGGGGAATTTTACCTCAGTTGAGGAGATGAACAAACCTAACAGCAAGTTACCATGGGGGAATCATATCTCACTCTTGCATGTACCAATACCCAAGTTATTAACGAACAGTGAAGAACAATTTCCAGATAATGCGCTTGACTTTGTCTGGAAGGCACATAAAATTATAAGGAGCAAAAGACATTCTTGGGGTATTTATCTCACAGCTGGATTCTTGGAGATTCTCAACAAATTTGGAGGCCATGAGGCGGCAGCAAAATACATCCGTAAATCATTGAAGAATTCGAGCATGATAATTTCAAATATGATTGGACCTGTGGAACAAATGTCTTTGGCAAATCATCCGATTAAAGGCTTATATTTTTTGGTGTTTGGTTCACCTGAGGGCCTTGATGTAACAATAGTAAGTTACATGGGAAAGGTGAGGATTGCCTTCAAGATGGAAAAGGGTCTTATTGATCCACCAAAGTTCAAGTCATGCATGGAAAAGTCCTTTGAAATGATACTCAAAGCCTCGGAGAACAATGCCGTGCAGAACAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1298 WES_acyltransf

Protein Analysis

466

Amino Acids

52.34

Weight (kDa)

9.0

Isoelectric Point (pI)

46.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WS_DGAT_cat PF03007 67 - 250 1.3e-11 Wax ester synthase/diacylglycerol acyltransferase catalytic domain
WS_DGAT_C PF06974 304 - 453 4.8e-37 WS/DGAT C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000634)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10290 FvH4_3g28230 FvH4_3g28240
malus_domestica MD05G1036400.v1.1 MD10G1159400.v1.1 MD17G1237100.v1.1 MD17G1237200.v1.1 MD17G1237300.v1.1 MD17G1237600.v1.1 MD17G1238200.v1.1
prunus_persica Prupe.3G140400_v2.0.a1 Prupe.3G140700_v2.0.a1 Prupe.3G141100_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G258100_v2.0.a1 Prupe.7G117300_v2.0.a1 Prupe.7G117400_v2.0.a1 Prupe.7G117600_v2.0.a1 Prupe.8G044700_v2.0.a1
pyrus_communis pycom05g02680 pycom17g24220 pycom17g24240
rosa_chinensis RchiOBHm_Chr1g0350141 RchiOBHm_Chr5g0050931 RchiOBHm_Chr5g0051001 RchiOBHm_Chr5g0051021 RchiOBHm_Chr6g0267491
rosa_laevigata RLG00000013999 RLG00000028502 RLG00000034744 RLG00000034748 RLG00000034753
rosa_multiflora Rmu_sc0000446.1_g000056 Rmu_sc0000782.1_g000006 Rmu_sc0002951.1_g000001 Rmu_sc0003674.1_g000026 Rmu_sc0003756.1_g000004 Rmu_sc0011917.1_g000004 Rmu_sc0012005.1_g000019 Rmu_sc0040739.1_g000001
rosa_roxburghii Rroxscaffold_1G00029730 Rroxscaffold_1G00029740 Rroxscaffold_4G00305060 Rroxscaffold_7G00200710
rosa_rugosa Rorug01G0208400 Rorug05G0265200.1 Rorug05G0265300.1 Rorug05G0265400.1 Rorug05G0265500.1 Rorug05G0294000 Rorug06G0033100 Rorug06G0243300
rosa_samantha Rh1BG192100 Rh1BG192200 Rh1DG220600 Rh5AG270300 Rh5AG337200 Rh5AG337700 Rh5BG346700 Rh5BG347400 Rh5CG374300 Rh5DG359700 Rh5DG360500 Rh6AG154500 Rh6BG155100 Rh6BG362300 Rh6CG150300 Rh6DG140300 Rh6DG356300
rosa_wichuraiana Rw0G018820 Rw1G019350 Rw5G025300 Rw5G031770 Rw5G031820 Rw5G031870 Rw5G031910 Rw6G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1022
AccI GTMKAC 2 cut(s) 466, 576
AccII CGCG 1 cut(s) 758
AciI CCGC 2 cut(s) 756, 1103
AclWI GGATC 1 cut(s) 1307
AcsI RAATTY 3 cut(s) 862, 1085, 1135
AfaI GTAC 4 cut(s) 50, 374, 397, 939
AfiI CCNNNNNNNGG 2 cut(s) 1102, 1205
AjnI CCWGG 1 cut(s) 768
AluBI AGCT 6 cut(s) 118, 229, 316, 412, 476, 1061
AluI AGCT 6 cut(s) 118, 229, 316, 412, 476, 1061
Alw21I GWGCWC 1 cut(s) 454
Alw26I GTCTC 1 cut(s) 182
AlwI GGATC 1 cut(s) 1307
AoxI GGCC 3 cut(s) 344, 1093, 1240
ApeKI GCWGC 3 cut(s) 25, 389, 1106
ApoI RAATTY 3 cut(s) 862, 1085, 1135
ArsI GACNNNNNNTTYG 2 cut(s) 1313, 1345
Asp700I GAANNNNTTC 2 cut(s) 330, 975
AspLEI GCGC 1 cut(s) 991
AspS9I GGNCC 2 cut(s) 1166, 1240
AsuHPI GGTGA 4 cut(s) 407, 440, 1224, 1288
AsuNHI GCTAGC 1 cut(s) 316
AvaII GGWCC 1 cut(s) 1166
AxyI CCTNAGG 1 cut(s) 1236
BanII GRGCYC 1 cut(s) 38
BbsI GAAGAC 1 cut(s) 240
Bbv12I GWGCWC 1 cut(s) 454
BbvI GCAGC 3 cut(s) 37, 401, 1118
BccI CCATC 5 cut(s) 164, 175, 425, 644, 1288
BceAI ACGGC 1 cut(s) 1367
BciT130I CCWGG 1 cut(s) 770
BclI TGATCA 1 cut(s) 622
BcoDI GTCTC 1 cut(s) 182
BfaI CTAG 1 cut(s) 317
BfmI CTRYAG 1 cut(s) 23
BisI GCNGC 4 cut(s) 26, 390, 1104, 1107
BlsI GCNGC 4 cut(s) 27, 391, 1105, 1108
BmcAI AGTACT 1 cut(s) 50
Bme1390I CCNGG 1 cut(s) 770
Bme18I GGWCC 1 cut(s) 1166
BmgT120I GGNCC 2 cut(s) 1166, 1240
BmiI GGNNCC 1 cut(s) 105
BmrFI CCNGG 1 cut(s) 770
BmsI GCATC 2 cut(s) 287, 843
BmtI GCTAGC 1 cut(s) 320
BpiI GAAGAC 1 cut(s) 240
BsaBI GATNNNNATC 2 cut(s) 291, 362
BsaJI CCNNGG 5 cut(s) 424, 530, 769, 908, 1368
Bsc4I CCNNNNNNNGG 2 cut(s) 1102, 1205
Bse118I RCCGGY 1 cut(s) 392
Bse1I ACTGG 4 cut(s) 29, 122, 384, 810
Bse21I CCTNAGG 1 cut(s) 1236
Bse3DI GCAATG 1 cut(s) 212
Bse8I GATNNNNATC 2 cut(s) 291, 362
BseBI CCWGG 1 cut(s) 770
BseDI CCNNGG 5 cut(s) 424, 530, 769, 908, 1368
BseGI GGATG 4 cut(s) 246, 858, 1116, 1195
BseJI GATNNNNATC 2 cut(s) 291, 362
BseLI CCNNNNNNNGG 2 cut(s) 1102, 1205
BseMI GCAATG 1 cut(s) 212
BseMII CTCAG 3 cut(s) 468, 885, 1227
BseNI ACTGG 4 cut(s) 29, 122, 384, 810
BseRI GAGGAG 2 cut(s) 163, 893
BseXI GCAGC 3 cut(s) 37, 401, 1118
BseYI CCCAGC 1 cut(s) 856
BsgI GTGCAG 1 cut(s) 408
Bsh1236I CGCG 1 cut(s) 758
Bsh1285I CGRYCG 1 cut(s) 289
BshFI GGCC 3 cut(s) 346, 1095, 1242
BsiEI CGRYCG 1 cut(s) 289
BsiHKAI GWGCWC 1 cut(s) 454
BsiSI CCGG 2 cut(s) 264, 393
BslFI GGGAC 2 cut(s) 787, 838
BslI CCNNNNNNNGG 2 cut(s) 1102, 1205
BsmAI GTCTC 1 cut(s) 182
BsmFI GGGAC 2 cut(s) 787, 838
BsnI GGCC 3 cut(s) 346, 1095, 1242
Bsp1286I GDGCHC 2 cut(s) 38, 454
Bsp143I GATC 6 cut(s) 286, 622, 638, 700, 762, 1312
Bsp19I CCATGG 1 cut(s) 908
BspACI CCGC 2 cut(s) 756, 1103
BspANI GGCC 3 cut(s) 346, 1095, 1242
BspCNI CTCAG 3 cut(s) 469, 884, 1228
BspFNI CGCG 1 cut(s) 758
BspLI GGNNCC 1 cut(s) 105
BspMAI CTGCAG 1 cut(s) 27
BspOI GCTAGC 1 cut(s) 320
BspPI GGATC 1 cut(s) 1307
BspQI GCTCTTC 1 cut(s) 459
BsrDI GCAATG 1 cut(s) 212
BsrFI RCCGGY 1 cut(s) 392
BsrI ACTGG 4 cut(s) 29, 122, 384, 810
BssAI RCCGGY 1 cut(s) 392
BssECI CCNNGG 5 cut(s) 424, 530, 769, 908, 1368
BssMI GATC 6 cut(s) 286, 622, 638, 700, 762, 1312
BssT1I CCWWGG 2 cut(s) 424, 908
Bst2UI CCWGG 1 cut(s) 770
Bst4CI ACNGT 2 cut(s) 62, 965
Bst6I CTCTTC 1 cut(s) 459
BstC8I GCNNGC 3 cut(s) 276, 318, 725
BstDEI CTNAG 3 cut(s) 477, 871, 1236
BstDSI CCRYGG 1 cut(s) 908
BstF5I GGATG 4 cut(s) 246, 858, 1116, 1195
BstFNI CGCG 1 cut(s) 758
BstHHI GCGC 1 cut(s) 991
BstKTI GATC 6 cut(s) 289, 625, 641, 703, 765, 1315
BstMAI GTCTC 1 cut(s) 182
BstMBI GATC 6 cut(s) 286, 622, 638, 700, 762, 1312
BstMCI CGRYCG 1 cut(s) 289
BstNI CCWGG 1 cut(s) 770
BstNSI RCATGY 2 cut(s) 790, 938
BstSCI CCNGG 1 cut(s) 768
BstSFI CTRYAG 1 cut(s) 23
BstUI CGCG 1 cut(s) 758
BstV1I GCAGC 3 cut(s) 37, 401, 1118
BstV2I GAAGAC 1 cut(s) 240
BstXI CCANNNNNNTGG 1 cut(s) 425
Bsu36I CCTNAGG 1 cut(s) 1236
BsuRI GGCC 3 cut(s) 346, 1095, 1242
BtgI CCRYGG 1 cut(s) 908
BtgZI GCGATG 2 cut(s) 507, 773
BtsCI GGATG 4 cut(s) 246, 858, 1116, 1195
BtsIMutI CAGTG 4 cut(s) 36, 391, 817, 970
Cac8I GCNNGC 3 cut(s) 276, 318, 725
CfoI GCGC 1 cut(s) 991
Cfr10I RCCGGY 1 cut(s) 392
Cfr13I GGNCC 2 cut(s) 1166, 1240
Csp6I GTAC 4 cut(s) 49, 373, 396, 938
CviQI GTAC 4 cut(s) 49, 373, 396, 938
DdeI CTNAG 3 cut(s) 477, 871, 1236
DpnI GATC 6 cut(s) 288, 624, 640, 702, 764, 1314
DpnII GATC 6 cut(s) 286, 622, 638, 700, 762, 1312
DraI TTTAAA 1 cut(s) 600
Eam1104I CTCTTC 1 cut(s) 459
EarI CTCTTC 1 cut(s) 459
Eco130I CCWWGG 2 cut(s) 424, 908
Eco147I AGGCCT 1 cut(s) 346
Eco24I GRGCYC 1 cut(s) 38
Eco47I GGWCC 1 cut(s) 1166
Eco81I CCTNAGG 1 cut(s) 1236
EcoO109I RGGNCCY 1 cut(s) 1240
EcoRI GAATTC 1 cut(s) 1135
EcoRII CCWGG 1 cut(s) 768
EcoT14I CCWWGG 2 cut(s) 424, 908
EcoT22I ATGCAT 1 cut(s) 1337
EcoT38I GRGCYC 1 cut(s) 38
ErhI CCWWGG 2 cut(s) 424, 908
FalI AAGNNNNNCTT 4 cut(s) 213, 245, 1024, 1056
FaqI GGGAC 2 cut(s) 787, 838
FbaI TGATCA 1 cut(s) 622
FblI GTMKAC 2 cut(s) 466, 576
Fnu4HI GCNGC 4 cut(s) 26, 390, 1104, 1107
FokI GGATG 4 cut(s) 233, 865, 1103, 1182
FriOI GRGCYC 1 cut(s) 38
Fsp4HI GCNGC 4 cut(s) 26, 390, 1104, 1107
FspBI CTAG 1 cut(s) 317
GlaI GCGC 1 cut(s) 990
GluI GCNGC 4 cut(s) 26, 390, 1104, 1107
GsaI CCCAGC 1 cut(s) 860
HaeIII GGCC 3 cut(s) 346, 1095, 1242
HapII CCGG 2 cut(s) 264, 393
HhaI GCGC 1 cut(s) 991
Hin6I GCGC 1 cut(s) 989
HinP1I GCGC 1 cut(s) 989
HindIII AAGCTT 1 cut(s) 410
HinfI GANTC 7 cut(s) 358, 481, 705, 775, 916, 1065, 1075
HpaII CCGG 2 cut(s) 264, 393
HphI GGTGA 4 cut(s) 407, 440, 1224, 1288
Hpy166II GTNNAC 4 cut(s) 244, 467, 577, 1232
Hpy188I TCNGA 5 cut(s) 592, 638, 731, 1200, 1372
Hpy188III TCNNGA 9 cut(s) 250, 296, 335, 510, 698, 718, 980, 1003, 1291
Hpy8I GTNNAC 4 cut(s) 244, 467, 577, 1232
HpyAV CCTTC 7 cut(s) 11, 270, 495, 500, 511, 1000, 1297
HpyCH4III ACNGT 2 cut(s) 62, 965
HpyCH4V TGCA 7 cut(s) 25, 389, 674, 790, 934, 1335, 1387
HpyF3I CTNAG 3 cut(s) 477, 871, 1236
HspAI GCGC 1 cut(s) 989
Ksp22I TGATCA 1 cut(s) 622
Kzo9I GATC 6 cut(s) 286, 622, 638, 700, 762, 1312
LguI GCTCTTC 1 cut(s) 459
LmnI GCTCC 2 cut(s) 226, 1026
Lsp1109I GCAGC 3 cut(s) 37, 401, 1118
LweI GCATC 2 cut(s) 287, 843
MaeI CTAG 1 cut(s) 317
MaeIII GTNAC 5 cut(s) 553, 733, 903, 1249, 1262
MalI GATC 6 cut(s) 288, 624, 640, 702, 764, 1314
MboI GATC 6 cut(s) 286, 622, 638, 700, 762, 1312
MboII GAAGA 8 cut(s) 23, 26, 245, 446, 629, 757, 980, 1144
MfeI CAATTG 1 cut(s) 669
MhlI GDGCHC 2 cut(s) 38, 454
MluCI AATT 9 cut(s) 96, 654, 669, 862, 974, 1017, 1085, 1135, 1149
MlyI GAGTC 2 cut(s) 699, 769
Mph1103I ATGCAT 1 cut(s) 1337
MroXI GAANNNNTTC 2 cut(s) 330, 975
MseI TTAA 4 cut(s) 599, 798, 956, 1203
MslI CAYNNNNRTG 1 cut(s) 851
MspA1I CMGCKG 1 cut(s) 1061
MspI CCGG 2 cut(s) 264, 393
MspR9I CCNGG 1 cut(s) 770
MunI CAATTG 1 cut(s) 669
MvaI CCWGG 1 cut(s) 770
MvnI CGCG 1 cut(s) 758
NcoI CCATGG 1 cut(s) 908
NdeII GATC 6 cut(s) 286, 622, 638, 700, 762, 1312
NheI GCTAGC 1 cut(s) 316
NlaIV GGNNCC 1 cut(s) 105
NmuCI GTSAC 2 cut(s) 553, 733
NsiI ATGCAT 1 cut(s) 1337
NspI RCATGY 2 cut(s) 790, 938
PceI AGGCCT 1 cut(s) 346
PciSI GCTCTTC 1 cut(s) 459
PcsI WCGNNNNNNNCGW 1 cut(s) 686
PdmI GAANNNNTTC 2 cut(s) 330, 975
PfeI GAWTC 5 cut(s) 358, 481, 916, 1065, 1075
PflFI GACNNNGTC 1 cut(s) 998
PkrI GCNGC 4 cut(s) 27, 391, 1105, 1108
Ple19I CGATCG 1 cut(s) 289
PleI GAGTC 2 cut(s) 699, 769
PpsI GAGTC 2 cut(s) 699, 769
PsiI TTATAA 1 cut(s) 1022
Psp6I CCWGG 1 cut(s) 768
PspFI CCCAGC 1 cut(s) 856
PspGI CCWGG 1 cut(s) 768
PspN4I GGNNCC 1 cut(s) 105
PspPI GGNCC 2 cut(s) 1166, 1240
PstI CTGCAG 1 cut(s) 27
PsyI GACNNNGTC 1 cut(s) 998
PvuI CGATCG 1 cut(s) 289
PvuII CAGCTG 1 cut(s) 1061
RsaI GTAC 4 cut(s) 50, 374, 397, 939
RsaNI GTAC 4 cut(s) 49, 373, 396, 938
RseI CAYNNNNRTG 1 cut(s) 851
SapI GCTCTTC 1 cut(s) 459
SaqAI TTAA 4 cut(s) 599, 798, 956, 1203
SatI GCNGC 4 cut(s) 26, 390, 1104, 1107
Sau3AI GATC 6 cut(s) 286, 622, 638, 700, 762, 1312
Sau96I GGNCC 2 cut(s) 1166, 1240
ScaI AGTACT 1 cut(s) 50
SchI GAGTC 2 cut(s) 699, 769
ScrFI CCNGG 1 cut(s) 770
SduI GDGCHC 2 cut(s) 38, 454
SfaNI GCATC 2 cut(s) 287, 843
SfcI CTRYAG 1 cut(s) 23
SinI GGWCC 1 cut(s) 1166
SmiMI CAYNNNNRTG 1 cut(s) 851
Sse9I AATT 9 cut(s) 96, 654, 669, 862, 974, 1017, 1085, 1135, 1149
SseBI AGGCCT 1 cut(s) 346
SsiI CCGC 2 cut(s) 756, 1103
SspI AATATT 1 cut(s) 405
SspMI CTAG 1 cut(s) 317
StuI AGGCCT 1 cut(s) 346
StyD4I CCNGG 1 cut(s) 768
StyI CCWWGG 2 cut(s) 424, 908
TaaI ACNGT 2 cut(s) 62, 965
TaqI TCGA 4 cut(s) 680, 703, 778, 1139
TaqII GACCGA 1 cut(s) 32
TasI AATT 9 cut(s) 96, 654, 669, 862, 974, 1017, 1085, 1135, 1149
TatI WGTACW 1 cut(s) 48
TauI GCSGC 1 cut(s) 1106
TfiI GAWTC 5 cut(s) 358, 481, 916, 1065, 1075
Tru1I TTAA 4 cut(s) 599, 798, 956, 1203
Tru9I TTAA 4 cut(s) 599, 798, 956, 1203
TscAI CASTG 4 cut(s) 36, 391, 817, 970
TseFI GTSAC 2 cut(s) 553, 733
TseI GCWGC 3 cut(s) 25, 389, 1106
Tsp45I GTSAC 2 cut(s) 553, 733
TspDTI ATGAA 1 cut(s) 899
TspGWI ACGGA 2 cut(s) 562, 1109
TspRI CASTG 4 cut(s) 36, 391, 817, 970
Tth111I GACNNNGTC 1 cut(s) 998
VpaK11BI GGWCC 1 cut(s) 1166
XapI RAATTY 3 cut(s) 862, 1085, 1135
XceI RCATGY 2 cut(s) 790, 938
XmiI GTMKAC 2 cut(s) 466, 576
XmnI GAANNNNTTC 2 cut(s) 330, 975
XspI CTAG 1 cut(s) 317
ZrmI AGTACT 1 cut(s) 50
Zsp2I ATGCAT 1 cut(s) 1337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.