Rh5AG337200

O-acyltransferase (WSD1-like)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000634
Physical Location & Seq
Reverse (-)
54290823 .. 54308440
17618 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG337200.1

Sequence Viewer

Length: 1410 bp
ATGGAGTTTGGCGAAGAAGGATTACTACAAGAGCCGGTGAGCCCTGGTGGTCAGTTCATCAACAGCTCTGTCTTATCTCTTGCAATTCTGGCCGTTTTGGAATCACAAGTTCCAATCGACGATTCTCAAACATTTTTTCTTCTGAAAAATGTCTTCCTCCCGATCAATCCACGCTTCTCTTCCATCATGGTTGAAGGTGCAGATAAAAAGAATAAGCAGTGGAAGAAGGTTACAGTGAAGCTGGAAGACCACGTCCATGTCCCCATTTTCCCCTCTGGAATATCATCACCTGCATCATACGATATTTACTTTGATGAGTATATAACGAAGATAGCATCAGAAACATTCCCACAAAGCAGGCCATTATGGGAACTTCATCTTTTCAAGTACCCAACAAGTCATGCAGCTGGACACATGATATTCAAGATCCACCATGCCCTTGGCGATGGCTACTCTCTCATGGGCGCTCTTCTCTCTTGCCTCCAGAATGCTCACAATCCTTCCATTCCGCTGACATTTCCGACATTAAAGAGTGCAAAAAATGAAACTAGTAGTCGATCTCGTGCGTTTGAGTTTATGCCGAAGATACTTTCTTCCGTTTTCAACGGTGCATGGGATTTCAGTTGGAGCATTTTGAAGGGCACTTGGGTTGAAGATGATCGAACACCAATAAGGTCCGGTGTTGAAGGAGTTGAGTTTCGACCTGTGTCGGTGTCAACCTTGATGCTGCCTATTGAGGAAATTAAACTTATCAAGAACAAGCTTGGAGTGACGATAAATGATGTGATTACAGGAACAATCTTTATGGGCATCCGAATGTACATTCAAGAGATGAATAGTGAAAAATCAAGTAGCCAAAATTGCACAGCACTGGTGTTGCTGAATACTAGACTAGTTGCGGGTTACAAGTCAGTGCAGGAGATGTTGATCGAAACAAATAAGTCTTGGGGAAACCGATTTGTGTTCTTGCAAGTCTCGGTGCCCAAGTCGAGTGAAGTTTCAAAGCCACTGGATTTTGTGTGGGAAGCACGCAATATAATCCAGAGACAGAGAAAATCTTCAGCTTGGTATCTCACCACTAGGCTCTGGGACATTTTGAAGAAATTTAGAGGCGCTGAGGCAGTATCTAAATATATCTATAGTACACTGAAAAACACAAGCATAGGAATCACAAATATGATTGGGCCATTGGAACAAATGAGTCTCGCCAATCACCCCATTGAAGGAATTTACTTCATAGTGACTGGTTCACCTCAGTGTCTTTCTGTAACAGCAATGAGTTATATGGGAAAGCTAAGGATTGCTTTGGTAGCTGAAAAAGACTTCATAGATACCAACGTATTGCAAGCATGCATGAAAGATGCATTTCGGGTGATAGGTGAAGCTGTGAATGAATTTCCTATAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1298 WES_acyltransf

Protein Analysis

469

Amino Acids

52.67

Weight (kDa)

8.45

Isoelectric Point (pI)

45.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WS_DGAT_cat PF03007 64 - 263 1.5e-11 Wax ester synthase/diacylglycerol acyltransferase catalytic domain
WS_DGAT_C PF06974 316 - 459 3e-35 WS/DGAT C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000634)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10290 FvH4_3g28230 FvH4_3g28240
malus_domestica MD05G1036400.v1.1 MD10G1159400.v1.1 MD17G1237100.v1.1 MD17G1237200.v1.1 MD17G1237300.v1.1 MD17G1237600.v1.1 MD17G1238200.v1.1
prunus_persica Prupe.3G140400_v2.0.a1 Prupe.3G140700_v2.0.a1 Prupe.3G141100_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G258100_v2.0.a1 Prupe.7G117300_v2.0.a1 Prupe.7G117400_v2.0.a1 Prupe.7G117600_v2.0.a1 Prupe.8G044700_v2.0.a1
pyrus_communis pycom05g02680 pycom17g24220 pycom17g24240
rosa_chinensis RchiOBHm_Chr1g0350141 RchiOBHm_Chr5g0050931 RchiOBHm_Chr5g0051001 RchiOBHm_Chr5g0051021 RchiOBHm_Chr6g0267491
rosa_laevigata RLG00000013999 RLG00000028502 RLG00000034744 RLG00000034748 RLG00000034753
rosa_multiflora Rmu_sc0000446.1_g000056 Rmu_sc0000782.1_g000006 Rmu_sc0002951.1_g000001 Rmu_sc0003674.1_g000026 Rmu_sc0003756.1_g000004 Rmu_sc0011917.1_g000004 Rmu_sc0012005.1_g000019 Rmu_sc0040739.1_g000001
rosa_roxburghii Rroxscaffold_1G00029730 Rroxscaffold_1G00029740 Rroxscaffold_4G00305060 Rroxscaffold_7G00200710
rosa_rugosa Rorug01G0208400 Rorug05G0265200.1 Rorug05G0265300.1 Rorug05G0265400.1 Rorug05G0265500.1 Rorug05G0294000 Rorug06G0033100 Rorug06G0243300
rosa_samantha Rh1BG192100 Rh1BG192200 Rh1DG220600 Rh5AG270300 Rh5AG337200 Rh5AG337700 Rh5BG346700 Rh5BG347400 Rh5CG374300 Rh5DG359700 Rh5DG360500 Rh6AG154500 Rh6BG155100 Rh6BG362300 Rh6CG150300 Rh6DG140300 Rh6DG356300
rosa_wichuraiana Rw0G018820 Rw1G019350 Rw5G025300 Rw5G031770 Rw5G031820 Rw5G031870 Rw5G031910 Rw6G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 298
Acc36I ACCTGC 1 cut(s) 298
AccB1I GGYRCC 1 cut(s) 979
AciI CCGC 2 cut(s) 509, 899
AclWI GGATC 1 cut(s) 421
AcoI YGGCCR 1 cut(s) 90
AcsI RAATTY 3 cut(s) 1103, 1227, 1394
AcuI CTGAAG 1 cut(s) 1044
AfaI GTAC 3 cut(s) 389, 821, 1144
AfiI CCNNNNNNNGG 1 cut(s) 1223
AhlI ACTAGT 2 cut(s) 548, 892
AjiI CACGTC 1 cut(s) 253
AjnI CCWGG 1 cut(s) 43
AjuI GAANNNNNNNTTGG 2 cut(s) 106, 138
AleI CACNNNNGTG 1 cut(s) 1255
AluBI AGCT 8 cut(s) 66, 241, 407, 763, 1064, 1294, 1313, 1385
AluI AGCT 8 cut(s) 66, 241, 407, 763, 1064, 1294, 1313, 1385
Alw26I GTCTC 3 cut(s) 979, 1039, 1208
AlwI GGATC 1 cut(s) 421
AoxI GGCC 3 cut(s) 90, 359, 1184
ApeKI GCWGC 2 cut(s) 404, 727
ApoI RAATTY 3 cut(s) 1103, 1227, 1394
Asp700I GAANNNNTTC 1 cut(s) 1057
AspLEI GCGC 2 cut(s) 467, 1115
AspS9I GGNCC 2 cut(s) 675, 1184
AsuHPI GGTGA 7 cut(s) 49, 279, 1066, 1205, 1242, 1384, 1391
AvaII GGWCC 1 cut(s) 675
BaeGI GKGCMC 2 cut(s) 644, 984
BaeI ACNNNNGTAYC 2 cut(s) 1323, 1356
BanI GGYRCC 1 cut(s) 979
BanII GRGCYC 1 cut(s) 44
BarI GAAGNNNNNNTAC 3 cut(s) 9, 38, 41
BauI CACGAG 1 cut(s) 561
BbsI GAAGAC 2 cut(s) 145, 252
BbvCI CCTCAGC 1 cut(s) 1116
BbvI GCAGC 2 cut(s) 416, 714
BccI CCATC 2 cut(s) 191, 440
BceAI ACGGC 1 cut(s) 77
BciT130I CCWGG 1 cut(s) 45
BcoDI GTCTC 3 cut(s) 979, 1039, 1208
BcuI ACTAGT 2 cut(s) 548, 892
BfaI CTAG 4 cut(s) 549, 888, 893, 1080
BfmI CTRYAG 1 cut(s) 1138
BfoI RGCGCY 2 cut(s) 468, 1116
BfuAI ACCTGC 1 cut(s) 298
BisI GCNGC 2 cut(s) 405, 728
BlsI GCNGC 2 cut(s) 406, 729
Bme1390I CCNGG 1 cut(s) 45
Bme18I GGWCC 1 cut(s) 675
BmgBI CACGTC 1 cut(s) 253
BmgT120I GGNCC 2 cut(s) 675, 1184
BmiI GGNNCC 1 cut(s) 981
BmrFI CCNGG 1 cut(s) 45
BmsI GCATC 5 cut(s) 302, 344, 714, 819, 1351
BoxI GACNNNNGTC 1 cut(s) 706
BpiI GAAGAC 2 cut(s) 145, 252
BpmI CTGGAG 1 cut(s) 467
Bpu10I CCTNAGC 2 cut(s) 1116, 1295
BsaBI GATNNNNATC 1 cut(s) 926
BsaJI CCNNGG 2 cut(s) 43, 439
BsaWI WCCGGW 1 cut(s) 677
Bsc4I CCNNNNNNNGG 1 cut(s) 1223
Bse118I RCCGGY 1 cut(s) 34
Bse1I ACTGG 3 cut(s) 876, 1014, 1249
Bse3DI GCAATG 1 cut(s) 1281
Bse8I GATNNNNATC 1 cut(s) 926
BseBI CCWGG 1 cut(s) 45
BseDI CCNNGG 2 cut(s) 43, 439
BseGI GGATG 1 cut(s) 810
BseJI GATNNNNATC 1 cut(s) 926
BseLI CCNNNNNNNGG 1 cut(s) 1223
BseMI GCAATG 1 cut(s) 1281
BseMII CTCAG 2 cut(s) 1107, 1268
BseNI ACTGG 3 cut(s) 876, 1014, 1249
BseSI GKGCMC 2 cut(s) 644, 984
BseXI GCAGC 2 cut(s) 416, 714
BsgI GTGCAG 2 cut(s) 219, 935
BshFI GGCC 3 cut(s) 92, 361, 1186
BshNI GGYRCC 1 cut(s) 979
BsiSI CCGG 2 cut(s) 35, 678
BslFI GGGAC 2 cut(s) 245, 1103
BslI CCNNNNNNNGG 1 cut(s) 1223
BsmAI GTCTC 3 cut(s) 979, 1039, 1208
BsmFI GGGAC 2 cut(s) 245, 1103
BsmI GAATGC 1 cut(s) 493
BsnI GGCC 3 cut(s) 92, 361, 1186
Bsp1286I GDGCHC 3 cut(s) 44, 644, 984
Bsp1407I TGTACA 1 cut(s) 819
Bsp143I GATC 5 cut(s) 162, 426, 557, 658, 927
BspACI CCGC 2 cut(s) 509, 899
BspANI GGCC 3 cut(s) 92, 361, 1186
BspCNI CTCAG 2 cut(s) 1108, 1267
BspLI GGNNCC 1 cut(s) 981
BspMI ACCTGC 1 cut(s) 298
BspPI GGATC 1 cut(s) 421
BspQI GCTCTTC 1 cut(s) 474
BspT107I GGYRCC 1 cut(s) 979
BsrDI GCAATG 1 cut(s) 1281
BsrFI RCCGGY 1 cut(s) 34
BsrGI TGTACA 1 cut(s) 819
BsrI ACTGG 3 cut(s) 876, 1014, 1249
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 2 cut(s) 43, 439
BssMI GATC 5 cut(s) 162, 426, 557, 658, 927
BssSI CACGAG 1 cut(s) 561
BssT1I CCWWGG 1 cut(s) 439
Bst2BI CACGAG 1 cut(s) 561
Bst2UI CCWGG 1 cut(s) 45
Bst4CI ACNGT 2 cut(s) 235, 608
Bst6I CTCTTC 2 cut(s) 184, 474
BstAUI TGTACA 1 cut(s) 819
BstC8I GCNNGC 4 cut(s) 359, 1030, 1347, 1351
BstDEI CTNAG 3 cut(s) 1116, 1254, 1295
BstF5I GGATG 1 cut(s) 810
BstH2I RGCGCY 2 cut(s) 468, 1116
BstHHI GCGC 2 cut(s) 467, 1115
BstKTI GATC 5 cut(s) 165, 429, 560, 661, 930
BstMAI GTCTC 3 cut(s) 979, 1039, 1208
BstMBI GATC 5 cut(s) 162, 426, 557, 658, 927
BstMWI GCNNNNNNNGC 3 cut(s) 89, 861, 1310
BstNI CCWGG 1 cut(s) 45
BstNSI RCATGY 1 cut(s) 1353
BstPAI GACNNNNGTC 1 cut(s) 706
BstSCI CCNGG 1 cut(s) 43
BstSFI CTRYAG 1 cut(s) 1138
BstSLI GKGCMC 2 cut(s) 644, 984
BstV1I GCAGC 2 cut(s) 416, 714
BstV2I GAAGAC 2 cut(s) 145, 252
BstX2I RGATCY 1 cut(s) 426
BstXI CCANNNNNNTGG 1 cut(s) 440
BstYI RGATCY 1 cut(s) 426
BsuRI GGCC 3 cut(s) 92, 361, 1186
BtgZI GCGATG 1 cut(s) 459
BtrI CACGTC 1 cut(s) 253
BtsCI GGATG 1 cut(s) 810
BtsI GCAGTG 1 cut(s) 224
BtsIMutI CAGTG 7 cut(s) 224, 240, 869, 918, 1007, 1145, 1262
BveI ACCTGC 1 cut(s) 298
Cac8I GCNNGC 4 cut(s) 359, 1030, 1347, 1351
CfoI GCGC 2 cut(s) 467, 1115
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 2 cut(s) 675, 1184
Csp6I GTAC 3 cut(s) 388, 820, 1143
CviAII CATG 9 cut(s) 187, 257, 401, 415, 434, 460, 612, 1350, 1354
CviQI GTAC 3 cut(s) 388, 820, 1143
DdeI CTNAG 3 cut(s) 1116, 1254, 1295
DpnI GATC 5 cut(s) 164, 428, 559, 660, 929
DpnII GATC 5 cut(s) 162, 426, 557, 658, 927
EaeI YGGCCR 1 cut(s) 90
Eam1104I CTCTTC 2 cut(s) 184, 474
EarI CTCTTC 2 cut(s) 184, 474
Eco130I CCWWGG 1 cut(s) 439
Eco24I GRGCYC 1 cut(s) 44
Eco47I GGWCC 1 cut(s) 675
Eco57I CTGAAG 1 cut(s) 1044
EcoRII CCWGG 1 cut(s) 43
EcoT14I CCWWGG 1 cut(s) 439
EcoT22I ATGCAT 2 cut(s) 1355, 1366
EcoT38I GRGCYC 1 cut(s) 44
ErhI CCWWGG 1 cut(s) 439
FaeI CATG 9 cut(s) 190, 260, 404, 418, 437, 463, 615, 1353, 1357
FalI AAGNNNNNCTT 2 cut(s) 1288, 1320
FaqI GGGAC 2 cut(s) 245, 1103
FatI CATG 9 cut(s) 186, 256, 400, 414, 433, 459, 611, 1349, 1353
FauI CCCGC 1 cut(s) 892
Fnu4HI GCNGC 2 cut(s) 405, 728
FokI GGATG 1 cut(s) 797
FriOI GRGCYC 1 cut(s) 44
Fsp4HI GCNGC 2 cut(s) 405, 728
FspBI CTAG 4 cut(s) 549, 888, 893, 1080
GlaI GCGC 2 cut(s) 466, 1114
GluI GCNGC 2 cut(s) 405, 728
GsuI CTGGAG 1 cut(s) 467
HaeII RGCGCY 2 cut(s) 468, 1116
HaeIII GGCC 3 cut(s) 92, 361, 1186
HapII CCGG 2 cut(s) 35, 678
HhaI GCGC 2 cut(s) 467, 1115
Hin1II CATG 9 cut(s) 190, 260, 404, 418, 437, 463, 615, 1353, 1357
Hin6I GCGC 2 cut(s) 465, 1113
HinP1I GCGC 2 cut(s) 465, 1113
HincII GTYRAC 1 cut(s) 717
HindII GTYRAC 1 cut(s) 717
HindIII AAGCTT 1 cut(s) 761
HinfI GANTC 4 cut(s) 101, 122, 1167, 1201
HpaII CCGG 2 cut(s) 35, 678
HphI GGTGA 7 cut(s) 49, 279, 1066, 1205, 1242, 1384, 1391
Hpy166II GTNNAC 3 cut(s) 717, 1145, 1250
Hpy188I TCNGA 4 cut(s) 144, 340, 522, 815
Hpy188III TCNNGA 7 cut(s) 160, 276, 424, 484, 754, 827, 1042
Hpy8I GTNNAC 3 cut(s) 717, 1145, 1250
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 7 cut(s) 11, 188, 220, 510, 631, 680, 1217
HpyCH4III ACNGT 2 cut(s) 235, 608
HpyCH4IV ACGT 2 cut(s) 252, 1338
HpyF10VI GCNNNNNNNGC 3 cut(s) 89, 861, 1310
HpyF3I CTNAG 3 cut(s) 1116, 1254, 1295
HpySE526I ACGT 2 cut(s) 252, 1338
Hsp92II CATG 9 cut(s) 190, 260, 404, 418, 437, 463, 615, 1353, 1357
HspAI GCGC 2 cut(s) 465, 1113
Kzo9I GATC 5 cut(s) 162, 426, 557, 658, 927
LguI GCTCTTC 1 cut(s) 474
LmnI GCTCC 1 cut(s) 627
Lsp1109I GCAGC 2 cut(s) 416, 714
LweI GCATC 5 cut(s) 302, 344, 714, 819, 1351
MaeI CTAG 4 cut(s) 549, 888, 893, 1080
MaeII ACGT 2 cut(s) 252, 1338
MaeIII GTNAC 5 cut(s) 229, 769, 902, 1240, 1267
MalI GATC 5 cut(s) 164, 428, 559, 660, 929
MboI GATC 5 cut(s) 162, 426, 557, 658, 927
MflI RGATCY 1 cut(s) 426
MhlI GDGCHC 3 cut(s) 44, 644, 984
MluCI AATT 6 cut(s) 84, 741, 859, 1103, 1227, 1394
MlyI GAGTC 1 cut(s) 1210
MmeI TCCRAC 2 cut(s) 545, 605
MnlI CCTC 7 cut(s) 167, 283, 491, 730, 1103, 1111, 1263
Mph1103I ATGCAT 2 cut(s) 1355, 1366
MroXI GAANNNNTTC 1 cut(s) 1057
MseI TTAA 2 cut(s) 527, 744
MslI CAYNNNNRTG 4 cut(s) 255, 815, 1175, 1255
MspA1I CMGCKG 2 cut(s) 407, 511
MspI CCGG 2 cut(s) 35, 678
MspR9I CCNGG 1 cut(s) 45
Mva1269I GAATGC 1 cut(s) 493
MvaI CCWGG 1 cut(s) 45
MwoI GCNNNNNNNGC 3 cut(s) 89, 861, 1310
NdeII GATC 5 cut(s) 162, 426, 557, 658, 927
NlaIII CATG 9 cut(s) 190, 260, 404, 418, 437, 463, 615, 1353, 1357
NlaIV GGNNCC 1 cut(s) 981
NmuCI GTSAC 2 cut(s) 769, 1240
NsiI ATGCAT 2 cut(s) 1355, 1366
NspI RCATGY 1 cut(s) 1353
OliI CACNNNNGTG 1 cut(s) 1255
PaeI GCATGC 1 cut(s) 1353
PaqCI CACCTGC 1 cut(s) 298
PciSI GCTCTTC 1 cut(s) 474
PctI GAATGC 1 cut(s) 493
PdmI GAANNNNTTC 1 cut(s) 1057
PfeI GAWTC 3 cut(s) 101, 122, 1167
PflFI GACNNNGTC 1 cut(s) 251
PkrI GCNGC 2 cut(s) 406, 729
PleI GAGTC 1 cut(s) 1209
PpsI GAGTC 1 cut(s) 1209
PshAI GACNNNNGTC 1 cut(s) 706
Psp6I CCWGG 1 cut(s) 43
PspGI CCWGG 1 cut(s) 43
PspN4I GGNNCC 1 cut(s) 981
PspPI GGNCC 2 cut(s) 675, 1184
PsuI RGATCY 1 cut(s) 426
PsyI GACNNNGTC 1 cut(s) 251
PvuII CAGCTG 1 cut(s) 407
RsaI GTAC 3 cut(s) 389, 821, 1144
RsaNI GTAC 3 cut(s) 388, 820, 1143
RseI CAYNNNNRTG 4 cut(s) 255, 815, 1175, 1255
SapI GCTCTTC 1 cut(s) 474
SaqAI TTAA 2 cut(s) 527, 744
SatI GCNGC 2 cut(s) 405, 728
Sau3AI GATC 5 cut(s) 162, 426, 557, 658, 927
Sau96I GGNCC 2 cut(s) 675, 1184
SchI GAGTC 1 cut(s) 1210
ScrFI CCNGG 1 cut(s) 45
SduI GDGCHC 3 cut(s) 44, 644, 984
SfaNI GCATC 5 cut(s) 302, 344, 714, 819, 1351
SfcI CTRYAG 1 cut(s) 1138
SinI GGWCC 1 cut(s) 675
SmiMI CAYNNNNRTG 4 cut(s) 255, 815, 1175, 1255
SpeI ACTAGT 2 cut(s) 548, 892
SphI GCATGC 1 cut(s) 1353
Sse9I AATT 6 cut(s) 84, 741, 859, 1103, 1227, 1394
SsiI CCGC 2 cut(s) 509, 899
SspMI CTAG 4 cut(s) 549, 888, 893, 1080
StyD4I CCNGG 1 cut(s) 43
StyI CCWWGG 1 cut(s) 439
TaaI ACNGT 2 cut(s) 235, 608
TaiI ACGT 2 cut(s) 255, 1341
TaqI TCGA 6 cut(s) 117, 556, 661, 700, 930, 989
TasI AATT 6 cut(s) 84, 741, 859, 1103, 1227, 1394
TatI WGTACW 2 cut(s) 819, 1142
TfiI GAWTC 3 cut(s) 101, 122, 1167
Tru1I TTAA 2 cut(s) 527, 744
Tru9I TTAA 2 cut(s) 527, 744
TscAI CASTG 7 cut(s) 224, 240, 876, 918, 1014, 1152, 1262
TseFI GTSAC 2 cut(s) 769, 1240
TseI GCWGC 2 cut(s) 404, 727
Tsp45I GTSAC 2 cut(s) 769, 1240
TspDTI ATGAA 8 cut(s) 46, 365, 558, 848, 1225, 1315, 1370, 1407
TspGWI ACGGA 1 cut(s) 586
TspRI CASTG 7 cut(s) 224, 240, 876, 918, 1014, 1152, 1262
Tth111I GACNNNGTC 1 cut(s) 251
VpaK11BI GGWCC 1 cut(s) 675
XapI RAATTY 3 cut(s) 1103, 1227, 1394
XceI RCATGY 1 cut(s) 1353
XcmI CCANNNNNNNNNTGG 1 cut(s) 437
XmnI GAANNNNTTC 1 cut(s) 1057
XspI CTAG 4 cut(s) 549, 888, 893, 1080
Zsp2I ATGCAT 2 cut(s) 1355, 1366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.