MD17G1237100.v1.1

O-acyltransferase (WSD1-like)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000634
Physical Location & Seq
Forward (+)
28603689 .. 28608973
5285 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1237100.v1.1.491

Sequence Viewer

Length: 1404 bp
ATGGAGATCGGGGGAGAGGGACTAGATCCAGTGAGCCCTACTGGCCACTACTTCAATAGCTCCGTTTTGTCGATATCGATTCTTGGTGTTTTAGAATTTGAAATTCCAATCGATGACTCTCAAACTCTATCATTGCTTGAGAATGTGTTTCTCCCCATCAACTCACGCTTCTCCTCTATAATGATTGAAAATAATGGAAAGAAGCAATGGAAAAAGGTTGAAGTGAAGCTTGAAGACCATGTTAAAAGTCCAATCTTCCCTTCCAACTTGTCAAATGAATCATATGACAAGTATTTTGATGACTATATCTCAAACATAGCAACAACAAGATTTCCAGAAGACAAACCACTGTGGGAAATTCATGTTATAAAGTACCCAAATAGCAATGCAGCTGGTAATGTAATATTCAAGTTGCACCATGCGTTAGGTGATGGCTACTCTCTCATGGGCGCTCTTATCTCCTGTCTACGAAGGGCTGACAATCCATCGCTTCCACTAACTTTTCCTTCACGACTGCCATCGAAACCGAAGAGTGAAAATGTTATGACCACAACTTTCTCATGCTTGTTGAACACCATATCCGATTCTTGGTGGGGCATTTCAAAGAGCATCATGGGGGAAGATGATCGATCACCAATAAAATCCTCGAACAATGGACTTGAGTTCGCGCCAATTACAGTATCTACTATGACGATCTCTCTTGATCTAATCAGATTAATTAAGAGCAGGCTTGGAGTGACAATAAATGATGTTCTTACTGGGGTGGTCTTTCTCGGCGCTCGACTATATATGCAAGAGATGAACAAAAGCTCAAGGGAAGCACATAGCACATCATTGGTTTTACTAAATACAAGGATCATGGCGAATTATACGTCGATTGAAGAAATGATCAAACCCGACAGCAAGAGCCCATGGGGAAATCGTTTTACATTCTTGCAAGTTCCTATTCCCAAGTTGACTGAATTCTCAAATGTGCTTGACTTCGTTTGGAAGGCACAAAAAATAATCAATCAGAAAAGAAATTCTTTTACTATTTATTTCATTAGTGGGCTATTGGAGATGCTGAACAAGTTTGGAGGCCATGAGGCAGCATCAAGATACATCCGTAGCACAATAAAGAACTCGAGCATGGTGATCACAAATATGATTGGCCCGCTGGAACAAATGTCTTTGGCAAACCATCCAATTAAAGGCTTATACTTTCTGGTAGTTGGGACTCCTCAGGACCTTGACATATCAATCCTAAGTTATATGGGAAAGGTGAAGCTTGCCTTCACAATGAAAAAAGGCATCATAGATCCACAAAAGTTGAAGTTGTGCATGGAAAACGCCCTCGATATGATATTCAACGCCTCAGACAGAAATTATATGCAAAATAGTACTGAAGAACAGTCACACGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1298 WES_acyltransf

Protein Analysis

468

Amino Acids

52.49

Weight (kDa)

7.13

Isoelectric Point (pI)

44.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WS_DGAT_cat PF03007 68 - 251 1.5e-09 Wax ester synthase/diacylglycerol acyltransferase catalytic domain
WS_DGAT_C PF06974 305 - 448 2e-39 WS/DGAT C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000634)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10290 FvH4_3g28230 FvH4_3g28240
malus_domestica MD05G1036400.v1.1 MD10G1159400.v1.1 MD17G1237100.v1.1 MD17G1237200.v1.1 MD17G1237300.v1.1 MD17G1237600.v1.1 MD17G1238200.v1.1
prunus_persica Prupe.3G140400_v2.0.a1 Prupe.3G140700_v2.0.a1 Prupe.3G141100_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G258100_v2.0.a1 Prupe.7G117300_v2.0.a1 Prupe.7G117400_v2.0.a1 Prupe.7G117600_v2.0.a1 Prupe.8G044700_v2.0.a1
pyrus_communis pycom05g02680 pycom17g24220 pycom17g24240
rosa_chinensis RchiOBHm_Chr1g0350141 RchiOBHm_Chr5g0050931 RchiOBHm_Chr5g0051001 RchiOBHm_Chr5g0051021 RchiOBHm_Chr6g0267491
rosa_laevigata RLG00000013999 RLG00000028502 RLG00000034744 RLG00000034748 RLG00000034753
rosa_multiflora Rmu_sc0000446.1_g000056 Rmu_sc0000782.1_g000006 Rmu_sc0002951.1_g000001 Rmu_sc0003674.1_g000026 Rmu_sc0003756.1_g000004 Rmu_sc0011917.1_g000004 Rmu_sc0012005.1_g000019 Rmu_sc0040739.1_g000001
rosa_roxburghii Rroxscaffold_1G00029730 Rroxscaffold_1G00029740 Rroxscaffold_4G00305060 Rroxscaffold_7G00200710
rosa_rugosa Rorug01G0208400 Rorug05G0265200.1 Rorug05G0265300.1 Rorug05G0265400.1 Rorug05G0265500.1 Rorug05G0294000 Rorug06G0033100 Rorug06G0243300
rosa_samantha Rh1BG192100 Rh1BG192200 Rh1DG220600 Rh5AG270300 Rh5AG337200 Rh5AG337700 Rh5BG346700 Rh5BG347400 Rh5CG374300 Rh5DG359700 Rh5DG360500 Rh6AG154500 Rh6BG155100 Rh6BG362300 Rh6CG150300 Rh6DG140300 Rh6DG356300
rosa_wichuraiana Rw0G018820 Rw1G019350 Rw5G025300 Rw5G031770 Rw5G031820 Rw5G031870 Rw5G031910 Rw6G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 368
AccI GTMKAC 1 cut(s) 466
AccII CGCG 1 cut(s) 668
AciI CCGC 1 cut(s) 1154
AclWI GGATC 3 cut(s) 20, 863, 1292
AcoI YGGCCR 1 cut(s) 43
AcsI RAATTY 5 cut(s) 95, 102, 357, 962, 1021
AcuI CTGAAG 1 cut(s) 1404
AfaI GTAC 2 cut(s) 374, 1381
AfiI CCNNNNNNNGG 2 cut(s) 588, 1190
AflIII ACRYGT 1 cut(s) 1396
AjuI GAANNNNNNNTTGG 2 cut(s) 244, 276
AloI GAACNNNNNNTCC 2 cut(s) 563, 595
AluBI AGCT 5 cut(s) 60, 229, 392, 810, 1267
AluI AGCT 5 cut(s) 60, 229, 392, 810, 1267
AlwI GGATC 3 cut(s) 20, 863, 1292
Ama87I CYCGRG 1 cut(s) 1123
AoxI GGCC 3 cut(s) 43, 1078, 1150
ApeKI GCWGC 2 cut(s) 389, 1088
ApoI RAATTY 5 cut(s) 95, 102, 357, 962, 1021
ArsI GACNNNNNNTTYG 4 cut(s) 278, 310, 857, 889
AseI ATTAAT 1 cut(s) 716
AspLEI GCGC 3 cut(s) 452, 670, 779
AspS9I GGNCC 2 cut(s) 1151, 1225
AsuHPI GGTGA 4 cut(s) 440, 624, 1144, 1273
AvaI CYCGRG 1 cut(s) 1123
AvaII GGWCC 1 cut(s) 1225
AxyI CCTNAGG 1 cut(s) 1221
BaeI ACNNNNGTAYC 2 cut(s) 1090, 1123
BalI TGGCCA 1 cut(s) 45
BanII GRGCYC 2 cut(s) 38, 911
BbsI GAAGAC 2 cut(s) 240, 345
BbvI GCAGC 2 cut(s) 401, 1100
BccI CCATC 5 cut(s) 164, 425, 493, 526, 1188
BclI TGATCA 2 cut(s) 888, 1134
BfaI CTAG 1 cut(s) 23
BfoI RGCGCY 2 cut(s) 453, 780
BglI GCCNNNNNGGC 1 cut(s) 42
BisI GCNGC 2 cut(s) 390, 1089
BlsI GCNGC 2 cut(s) 391, 1090
BmcAI AGTACT 1 cut(s) 1381
Bme18I GGWCC 1 cut(s) 1225
BmeT110I CYCGRG 1 cut(s) 1123
BmgT120I GGNCC 2 cut(s) 1151, 1225
BmrI ACTGGG 1 cut(s) 768
BmsI GCATC 4 cut(s) 618, 1050, 1100, 1299
BmuI ACTGGG 1 cut(s) 768
BpiI GAAGAC 2 cut(s) 240, 345
BpuEI CTTGAG 3 cut(s) 158, 680, 796
Bsa29I ATCGAT 3 cut(s) 77, 111, 628
BsaJI CCNNGG 1 cut(s) 911
Bsc4I CCNNNNNNNGG 2 cut(s) 588, 1190
Bse1I ACTGG 3 cut(s) 29, 46, 763
Bse21I CCTNAGG 1 cut(s) 1221
Bse3DI GCAATG 3 cut(s) 131, 212, 391
BseCI ATCGAT 3 cut(s) 77, 111, 628
BseDI CCNNGG 1 cut(s) 911
BseGI GGATG 2 cut(s) 1101, 1180
BseLI CCNNNNNNNGG 2 cut(s) 588, 1190
BseMI GCAATG 3 cut(s) 131, 212, 391
BseMII CTCAG 2 cut(s) 1235, 1368
BseNI ACTGG 3 cut(s) 29, 46, 763
BseRI GAGGAG 2 cut(s) 163, 1209
BseXI GCAGC 2 cut(s) 401, 1100
Bsh1236I CGCG 1 cut(s) 668
BshFI GGCC 3 cut(s) 45, 1080, 1152
BshVI ATCGAT 3 cut(s) 77, 111, 628
BsiHKCI CYCGRG 1 cut(s) 1123
BslFI GGGAC 2 cut(s) 33, 1228
BslI CCNNNNNNNGG 2 cut(s) 588, 1190
BsmFI GGGAC 2 cut(s) 33, 1228
BsnI GGCC 3 cut(s) 45, 1080, 1152
BsoBI CYCGRG 1 cut(s) 1123
Bsp1286I GDGCHC 2 cut(s) 38, 911
Bsp19I CCATGG 1 cut(s) 911
BspACI CCGC 1 cut(s) 1154
BspANI GGCC 3 cut(s) 45, 1080, 1152
BspCNI CTCAG 2 cut(s) 1234, 1367
BspDI ATCGAT 3 cut(s) 77, 111, 628
BspFNI CGCG 1 cut(s) 668
BspPI GGATC 3 cut(s) 20, 863, 1292
BsrDI GCAATG 3 cut(s) 131, 212, 391
BsrI ACTGG 3 cut(s) 29, 46, 763
BssECI CCNNGG 1 cut(s) 911
BssT1I CCWWGG 1 cut(s) 911
Bst4CI ACNGT 3 cut(s) 351, 679, 1392
Bst6I CTCTTC 1 cut(s) 524
BstC8I GCNNGC 3 cut(s) 728, 1154, 1269
BstDEI CTNAG 3 cut(s) 1221, 1244, 1354
BstDSI CCRYGG 1 cut(s) 911
BstF5I GGATG 2 cut(s) 1101, 1180
BstFNI CGCG 1 cut(s) 668
BstH2I RGCGCY 2 cut(s) 453, 780
BstHHI GCGC 3 cut(s) 452, 670, 779
BstMWI GCNNNNNNNGC 1 cut(s) 42
BstUI CGCG 1 cut(s) 668
BstV1I GCAGC 2 cut(s) 401, 1100
BstV2I GAAGAC 2 cut(s) 240, 345
BstX2I RGATCY 2 cut(s) 25, 1297
BstYI RGATCY 2 cut(s) 25, 1297
Bsu15I ATCGAT 3 cut(s) 77, 111, 628
Bsu36I CCTNAGG 1 cut(s) 1221
BsuRI GGCC 3 cut(s) 45, 1080, 1152
BsuTUI ATCGAT 3 cut(s) 77, 111, 628
BtgI CCRYGG 1 cut(s) 911
BtgZI GCGATG 1 cut(s) 471
BtsCI GGATG 2 cut(s) 1101, 1180
BtsIMutI CAGTG 2 cut(s) 36, 347
Cac8I GCNNGC 3 cut(s) 728, 1154, 1269
CfoI GCGC 3 cut(s) 452, 670, 779
Cfr13I GGNCC 2 cut(s) 1151, 1225
ClaI ATCGAT 3 cut(s) 77, 111, 628
Csp6I GTAC 2 cut(s) 373, 1380
CspCI CAANNNNNGTGG 2 cut(s) 1290, 1325
CviQI GTAC 2 cut(s) 373, 1380
DdeI CTNAG 3 cut(s) 1221, 1244, 1354
EaeI YGGCCR 1 cut(s) 43
Eam1104I CTCTTC 1 cut(s) 524
EarI CTCTTC 1 cut(s) 524
Eco130I CCWWGG 1 cut(s) 911
Eco24I GRGCYC 2 cut(s) 38, 911
Eco32I GATATC 1 cut(s) 75
Eco47I GGWCC 1 cut(s) 1225
Eco57I CTGAAG 1 cut(s) 1404
Eco81I CCTNAGG 1 cut(s) 1221
Eco88I CYCGRG 1 cut(s) 1123
EcoO109I RGGNCCY 1 cut(s) 1225
EcoRI GAATTC 1 cut(s) 962
EcoRV GATATC 1 cut(s) 75
EcoT14I CCWWGG 1 cut(s) 911
EcoT38I GRGCYC 2 cut(s) 38, 911
ErhI CCWWGG 1 cut(s) 911
FalI AAGNNNNNCTT 6 cut(s) 213, 245, 1009, 1041, 1256, 1288
FaqI GGGAC 2 cut(s) 33, 1228
FauI CCCGC 1 cut(s) 1161
FauNDI CATATG 1 cut(s) 283
FbaI TGATCA 2 cut(s) 888, 1134
FblI GTMKAC 1 cut(s) 466
Fnu4HI GCNGC 2 cut(s) 390, 1089
FokI GGATG 2 cut(s) 1088, 1167
FriOI GRGCYC 2 cut(s) 38, 911
Fsp4HI GCNGC 2 cut(s) 390, 1089
FspBI CTAG 1 cut(s) 23
GlaI GCGC 3 cut(s) 451, 669, 778
GluI GCNGC 2 cut(s) 390, 1089
HaeII RGCGCY 2 cut(s) 453, 780
HaeIII GGCC 3 cut(s) 45, 1080, 1152
HhaI GCGC 3 cut(s) 452, 670, 779
Hin6I GCGC 3 cut(s) 450, 668, 777
HinP1I GCGC 3 cut(s) 450, 668, 777
HincII GTYRAC 1 cut(s) 957
HindII GTYRAC 1 cut(s) 957
HindIII AAGCTT 2 cut(s) 227, 1265
HinfI GANTC 5 cut(s) 79, 116, 278, 584, 1216
HphI GGTGA 4 cut(s) 440, 624, 1144, 1273
Hpy166II GTNNAC 2 cut(s) 467, 957
Hpy188I TCNGA 4 cut(s) 583, 713, 1014, 1357
Hpy188III TCNNGA 5 cut(s) 335, 510, 701, 1095, 1223
Hpy8I GTNNAC 2 cut(s) 467, 957
Hpy99I CGWCG 1 cut(s) 877
HpyAV CCTTC 5 cut(s) 270, 465, 516, 985, 1282
HpyCH4III ACNGT 3 cut(s) 351, 679, 1392
HpyCH4IV ACGT 2 cut(s) 872, 1398
HpyCH4V TGCA 6 cut(s) 389, 415, 793, 937, 1320, 1372
HpyF10VI GCNNNNNNNGC 1 cut(s) 42
HpyF3I CTNAG 3 cut(s) 1221, 1244, 1354
HpySE526I ACGT 2 cut(s) 872, 1398
HspAI GCGC 3 cut(s) 450, 668, 777
Ksp22I TGATCA 2 cut(s) 888, 1134
LmnI GCTCC 1 cut(s) 65
Lsp1109I GCAGC 2 cut(s) 401, 1100
LweI GCATC 4 cut(s) 618, 1050, 1100, 1299
MaeI CTAG 1 cut(s) 23
MaeII ACGT 2 cut(s) 872, 1398
MaeIII GTNAC 2 cut(s) 736, 1392
MboII GAAGA 7 cut(s) 245, 247, 350, 541, 632, 893, 1397
MflI RGATCY 2 cut(s) 25, 1297
MhlI GDGCHC 2 cut(s) 38, 911
MlsI TGGCCA 1 cut(s) 45
MluNI TGGCCA 1 cut(s) 45
MlyI GAGTC 2 cut(s) 110, 1210
MmeI TCCRAC 1 cut(s) 288
MnlI CCTC 8 cut(s) 10, 184, 655, 1070, 1078, 1230, 1343, 1363
Mox20I TGGCCA 1 cut(s) 45
MscI TGGCCA 1 cut(s) 45
MseI TTAA 4 cut(s) 243, 716, 720, 1188
MslI CAYNNNNRTG 1 cut(s) 1142
Msp20I TGGCCA 1 cut(s) 45
MspA1I CMGCKG 2 cut(s) 392, 1156
MvnI CGCG 1 cut(s) 668
MwoI GCNNNNNNNGC 1 cut(s) 42
NcoI CCATGG 1 cut(s) 911
NdeI CATATG 1 cut(s) 283
NmeAIII GCCGAG 1 cut(s) 753
NmuCI GTSAC 2 cut(s) 736, 1392
PacI TTAATTAA 1 cut(s) 720
PaeR7I CTCGAG 1 cut(s) 1123
PfeI GAWTC 3 cut(s) 79, 278, 584
PkrI GCNGC 2 cut(s) 391, 1090
PleI GAGTC 2 cut(s) 110, 1210
PpsI GAGTC 2 cut(s) 110, 1210
PpuMI RGGWCCY 1 cut(s) 1225
PshBI ATTAAT 1 cut(s) 716
PsiI TTATAA 1 cut(s) 368
Psp5II RGGWCCY 1 cut(s) 1225
PspPI GGNCC 2 cut(s) 1151, 1225
PspPPI RGGWCCY 1 cut(s) 1225
PspXI VCTCGAGB 1 cut(s) 1123
PsuI RGATCY 2 cut(s) 25, 1297
PvuII CAGCTG 1 cut(s) 392
RsaI GTAC 2 cut(s) 374, 1381
RsaNI GTAC 2 cut(s) 373, 1380
RseI CAYNNNNRTG 1 cut(s) 1142
SaqAI TTAA 4 cut(s) 243, 716, 720, 1188
SatI GCNGC 2 cut(s) 390, 1089
Sau96I GGNCC 2 cut(s) 1151, 1225
ScaI AGTACT 1 cut(s) 1381
SchI GAGTC 2 cut(s) 110, 1210
SduI GDGCHC 2 cut(s) 38, 911
SfaNI GCATC 4 cut(s) 618, 1050, 1100, 1299
Sfr274I CTCGAG 1 cut(s) 1123
SinI GGWCC 1 cut(s) 1225
SlaI CTCGAG 1 cut(s) 1123
SmiMI CAYNNNNRTG 1 cut(s) 1142
SmlI CTYRAG 4 cut(s) 137, 659, 811, 1123
SmoI CTYRAG 4 cut(s) 137, 659, 811, 1123
SsiI CCGC 1 cut(s) 1154
SspI AATATT 1 cut(s) 405
SspMI CTAG 1 cut(s) 23
StyI CCWWGG 1 cut(s) 911
TaaI ACNGT 3 cut(s) 351, 679, 1392
TaiI ACGT 2 cut(s) 875, 1401
TatI WGTACW 1 cut(s) 1379
TfiI GAWTC 3 cut(s) 79, 278, 584
Tru1I TTAA 4 cut(s) 243, 716, 720, 1188
Tru9I TTAA 4 cut(s) 243, 716, 720, 1188
TscAI CASTG 2 cut(s) 36, 354
TseFI GTSAC 2 cut(s) 736, 1392
TseI GCWGC 2 cut(s) 389, 1088
Tsp45I GTSAC 2 cut(s) 736, 1392
TspDTI ATGAA 5 cut(s) 291, 350, 815, 1030, 1295
TspGWI ACGGA 2 cut(s) 52, 1094
TspRI CASTG 2 cut(s) 36, 354
VpaK11BI GGWCC 1 cut(s) 1225
VspI ATTAAT 1 cut(s) 716
XapI RAATTY 5 cut(s) 95, 102, 357, 962, 1021
XhoI CTCGAG 1 cut(s) 1123
XmiI GTMKAC 1 cut(s) 466
XspI CTAG 1 cut(s) 23
ZrmI AGTACT 1 cut(s) 1381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.