RchiOBHm_Chr5g0051021

O-acyltransferase (WSD1-like)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000634
Physical Location & Seq
Reverse (-)
51919173 .. 51920653
1481 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32854

Sequence Viewer

Length: 1131 bp
ATGGAGTTTGGCGAAGGAGGACTACTACAAGAGCCAGTGAGCCCGGGTAGTCAATTAGTCAACAGCTCTGTCTTATCTCTTGCAATTCTTGCCGTTTTGGAATCACAAGTTGCAGTCGACGATTCTCAAACTTTTTTGCTTCTTAAAAATGTCTTCCTCCCGATTAATCCACGCTTCTCTTCCATCATGGTTGAAGGTGTGGATGAAAAGAATAAGCAGTGGAAGAGGGTTGAAGTGAAGCTGGAAGACCATGTGCATGTCCCCATTTTCCCCACTGGAATGTCACTTGAATCATACGATACTTACTTTGATGAGTACATAACGAAGATAGCATCAGAAACATTCCCACAAAGCAGGCCATTGTGGGAACTTCATCTTTTCAAGTACCCAACAAGTCATGCAGCTGGACAGATAATATTCAAGATCCACCATGCCCTTGGCGATGGCTACTCTCTCATGGGTGCTCTTCTCTCTTGTCTCCAAAATGCTCAAAATCCTTCTCTTCCCCTGACATTTCCTTCACTAAAGGGTGCAAAGAATGAAGCTAATAGTCGATCCCGTGTGTTTGAGTTTGTGCCTAAGATATTTTCTGCCGTTATCAACGGTGCATGGGATTCATGTTGGAGCATTTTGACGGGCACTTGGGTTGAAGATGATCGAACACCAATAAGGTCCGGCGTTGATGGGGTTGAGTTTCGACCTTTGTCCGTATCGACCTTGATGCTGCCTATTGAGGAAATTAAACTTATCAAGAACAAGCTTGGAGTGACGATAAATGATGTGATTTCAGGAACAATCTTTCTGGGCATCCGAATGTACATTCAAGAGATGGATAATGAAAAATCAAGTAGCCAGAACTGCACCGCATTGGTGTTGCTGAATAGTAGACTTGCTGCGGGTTACAAGTCAGTGCAGGAGATGTTGATCGAAACAAACAAGTCTTGGGGAAACCGAATTGTGTTCTTGCCCGTCTCGGTGCCCAAGTCAAGTGAAGTTACAAAGCCACTGGATTTTGTGTGGGAAGCACATAATATAATCAAGAGACAGAGAAACTCTTCATCTTGGTACCTCACCACTAGGTTCCTGGATATTTTGAAGAAATTTAGAGGCCCTGAGGTAATTAATTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1298 WES_acyltransf

Protein Analysis

376

Amino Acids

42.28

Weight (kDa)

6.21

Isoelectric Point (pI)

48.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WS_DGAT_cat PF03007 70 - 262 4.6e-10 Wax ester synthase/diacylglycerol acyltransferase catalytic domain
WS_DGAT_C PF06974 315 - 373 9.5e-06 WS/DGAT C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000634)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10290 FvH4_3g28230 FvH4_3g28240
malus_domestica MD05G1036400.v1.1 MD10G1159400.v1.1 MD17G1237100.v1.1 MD17G1237200.v1.1 MD17G1237300.v1.1 MD17G1237600.v1.1 MD17G1238200.v1.1
prunus_persica Prupe.3G140400_v2.0.a1 Prupe.3G140700_v2.0.a1 Prupe.3G141100_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G258100_v2.0.a1 Prupe.7G117300_v2.0.a1 Prupe.7G117400_v2.0.a1 Prupe.7G117600_v2.0.a1 Prupe.8G044700_v2.0.a1
pyrus_communis pycom05g02680 pycom17g24220 pycom17g24240
rosa_chinensis RchiOBHm_Chr1g0350141 RchiOBHm_Chr5g0050931 RchiOBHm_Chr5g0051001 RchiOBHm_Chr5g0051021 RchiOBHm_Chr6g0267491
rosa_laevigata RLG00000013999 RLG00000028502 RLG00000034744 RLG00000034748 RLG00000034753
rosa_multiflora Rmu_sc0000446.1_g000056 Rmu_sc0000782.1_g000006 Rmu_sc0002951.1_g000001 Rmu_sc0003674.1_g000026 Rmu_sc0003756.1_g000004 Rmu_sc0011917.1_g000004 Rmu_sc0012005.1_g000019 Rmu_sc0040739.1_g000001
rosa_roxburghii Rroxscaffold_1G00029730 Rroxscaffold_1G00029740 Rroxscaffold_4G00305060 Rroxscaffold_7G00200710
rosa_rugosa Rorug01G0208400 Rorug05G0265200.1 Rorug05G0265300.1 Rorug05G0265400.1 Rorug05G0265500.1 Rorug05G0294000 Rorug06G0033100 Rorug06G0243300
rosa_samantha Rh1BG192100 Rh1BG192200 Rh1DG220600 Rh5AG270300 Rh5AG337200 Rh5AG337700 Rh5BG346700 Rh5BG347400 Rh5CG374300 Rh5DG359700 Rh5DG360500 Rh6AG154500 Rh6BG155100 Rh6BG362300 Rh6CG150300 Rh6DG140300 Rh6DG356300
rosa_wichuraiana Rw0G018820 Rw1G019350 Rw5G025300 Rw5G031770 Rw5G031820 Rw5G031870 Rw5G031910 Rw6G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1065
AccB1I GGYRCC 2 cut(s) 976, 1065
AccI GTMKAC 2 cut(s) 117, 886
AciI CCGC 2 cut(s) 864, 896
AclWI GGATC 2 cut(s) 418, 549
AcsI RAATTY 1 cut(s) 1100
AfaI GTAC 4 cut(s) 317, 386, 818, 1067
AgsI TTSAA 8 cut(s) 194, 233, 290, 382, 421, 650, 824, 1096
AjnI CCWGG 1 cut(s) 1083
AluBI AGCT 5 cut(s) 66, 241, 404, 545, 760
AluI AGCT 5 cut(s) 66, 241, 404, 545, 760
Alw21I GWGCWC 1 cut(s) 466
Alw26I GTCTC 3 cut(s) 482, 976, 1036
AlwI GGATC 2 cut(s) 418, 549
Ama87I CYCGRG 1 cut(s) 43
AoxI GGCC 2 cut(s) 356, 1108
ApeKI GCWGC 3 cut(s) 401, 724, 893
ApoI RAATTY 1 cut(s) 1100
AseI ATTAAT 2 cut(s) 165, 1122
Asp700I GAANNNNTTC 1 cut(s) 1054
Asp718I GGTACC 1 cut(s) 1065
AspS9I GGNCC 2 cut(s) 672, 1109
AsuC2I CCSGG 2 cut(s) 44, 45
AsuHPI GGTGA 1 cut(s) 1063
AvaI CYCGRG 1 cut(s) 43
AvaII GGWCC 1 cut(s) 672
AxyI CCTNAGG 1 cut(s) 1113
BaeGI GKGCMC 2 cut(s) 641, 981
BanI GGYRCC 2 cut(s) 976, 1065
BanII GRGCYC 1 cut(s) 44
BarI GAAGNNNNNNTAC 1 cut(s) 38
BbsI GAAGAC 2 cut(s) 145, 252
Bbv12I GWGCWC 1 cut(s) 466
BbvI GCAGC 3 cut(s) 413, 711, 880
BccI CCATC 4 cut(s) 191, 437, 677, 823
BceAI ACGGC 2 cut(s) 77, 578
BcgI CGANNNNNNTGC 2 cut(s) 703, 737
BciT130I CCWGG 1 cut(s) 1085
BcnI CCSGG 2 cut(s) 44, 45
BcoDI GTCTC 3 cut(s) 482, 976, 1036
BfaI CTAG 1 cut(s) 1077
BisI GCNGC 3 cut(s) 402, 725, 894
BlsI GCNGC 3 cut(s) 403, 726, 895
Bme1390I CCNGG 3 cut(s) 44, 45, 1085
Bme18I GGWCC 1 cut(s) 672
BmeT110I CYCGRG 1 cut(s) 43
BmgT120I GGNCC 2 cut(s) 672, 1109
BmiI GGNNCC 3 cut(s) 978, 1067, 1082
BmrFI CCNGG 3 cut(s) 44, 45, 1085
BmsI GCATC 3 cut(s) 341, 711, 816
BoxI GACNNNNGTC 1 cut(s) 703
BpiI GAAGAC 2 cut(s) 145, 252
BpuMI CCSGG 2 cut(s) 44, 45
BsaBI GATNNNNATC 1 cut(s) 923
BsaJI CCNNGG 2 cut(s) 43, 436
Bse1I ACTGG 3 cut(s) 35, 280, 1011
Bse21I CCTNAGG 1 cut(s) 1113
Bse8I GATNNNNATC 1 cut(s) 923
BseBI CCWGG 1 cut(s) 1085
BseDI CCNNGG 2 cut(s) 43, 436
BseGI GGATG 2 cut(s) 208, 807
BseJI GATNNNNATC 1 cut(s) 923
BseMII CTCAG 1 cut(s) 1104
BseNI ACTGG 3 cut(s) 35, 280, 1011
BseSI GKGCMC 2 cut(s) 641, 981
BseXI GCAGC 3 cut(s) 413, 711, 880
BsgI GTGCAG 2 cut(s) 844, 932
BshFI GGCC 2 cut(s) 358, 1110
BshNI GGYRCC 2 cut(s) 976, 1065
BsiHKAI GWGCWC 1 cut(s) 466
BsiHKCI CYCGRG 1 cut(s) 43
BsiSI CCGG 2 cut(s) 44, 675
BslFI GGGAC 1 cut(s) 245
BsmAI GTCTC 3 cut(s) 482, 976, 1036
BsmBI CGTCTC 1 cut(s) 976
BsmFI GGGAC 1 cut(s) 245
BsnI GGCC 2 cut(s) 358, 1110
BsoBI CYCGRG 1 cut(s) 43
Bsp1286I GDGCHC 4 cut(s) 44, 466, 641, 981
Bsp1407I TGTACA 1 cut(s) 816
Bsp143I GATC 4 cut(s) 423, 554, 655, 924
BspACI CCGC 2 cut(s) 864, 896
BspANI GGCC 2 cut(s) 358, 1110
BspCNI CTCAG 1 cut(s) 1105
BspLI GGNNCC 3 cut(s) 978, 1067, 1082
BspPI GGATC 2 cut(s) 418, 549
BspQI GCTCTTC 1 cut(s) 471
BspT107I GGYRCC 2 cut(s) 976, 1065
BsrGI TGTACA 1 cut(s) 816
BsrI ACTGG 3 cut(s) 35, 280, 1011
BssECI CCNNGG 2 cut(s) 43, 436
BssMI GATC 4 cut(s) 423, 554, 655, 924
BssT1I CCWWGG 1 cut(s) 436
Bst2UI CCWGG 1 cut(s) 1085
Bst4CI ACNGT 1 cut(s) 605
Bst6I CTCTTC 5 cut(s) 184, 218, 471, 507, 1060
BstAPI GCANNNNNTGC 1 cut(s) 89
BstAUI TGTACA 1 cut(s) 816
BstC8I GCNNGC 1 cut(s) 356
BstDEI CTNAG 2 cut(s) 579, 1113
BstF5I GGATG 2 cut(s) 208, 807
BstKTI GATC 4 cut(s) 426, 557, 658, 927
BstMAI GTCTC 3 cut(s) 482, 976, 1036
BstMBI GATC 4 cut(s) 423, 554, 655, 924
BstMWI GCNNNNNNNGC 2 cut(s) 89, 858
BstNI CCWGG 1 cut(s) 1085
BstNSI RCATGY 1 cut(s) 260
BstPAI GACNNNNGTC 1 cut(s) 703
BstSCI CCNGG 3 cut(s) 42, 43, 1083
BstSLI GKGCMC 2 cut(s) 641, 981
BstV1I GCAGC 3 cut(s) 413, 711, 880
BstV2I GAAGAC 2 cut(s) 145, 252
BstX2I RGATCY 1 cut(s) 423
BstXI CCANNNNNNTGG 1 cut(s) 437
BstYI RGATCY 1 cut(s) 423
Bsu36I CCTNAGG 1 cut(s) 1113
BsuRI GGCC 2 cut(s) 358, 1110
BtgZI GCGATG 1 cut(s) 456
BtsCI GGATG 2 cut(s) 208, 807
BtsI GCAGTG 1 cut(s) 224
BtsIMutI CAGTG 5 cut(s) 42, 224, 273, 915, 1004
Cac8I GCNNGC 1 cut(s) 356
Cfr13I GGNCC 2 cut(s) 672, 1109
Cfr9I CCCGGG 1 cut(s) 43
Csp6I GTAC 4 cut(s) 316, 385, 817, 1066
CviAII CATG 8 cut(s) 187, 251, 257, 398, 431, 457, 609, 618
CviQI GTAC 4 cut(s) 316, 385, 817, 1066
DdeI CTNAG 2 cut(s) 579, 1113
DpnI GATC 4 cut(s) 425, 556, 657, 926
DpnII GATC 4 cut(s) 423, 554, 655, 924
Eam1104I CTCTTC 5 cut(s) 184, 218, 471, 507, 1060
EarI CTCTTC 5 cut(s) 184, 218, 471, 507, 1060
Eco130I CCWWGG 1 cut(s) 436
Eco24I GRGCYC 1 cut(s) 44
Eco47I GGWCC 1 cut(s) 672
Eco81I CCTNAGG 1 cut(s) 1113
Eco88I CYCGRG 1 cut(s) 43
EcoO109I RGGNCCY 1 cut(s) 1109
EcoRII CCWGG 1 cut(s) 1083
EcoT14I CCWWGG 1 cut(s) 436
EcoT38I GRGCYC 1 cut(s) 44
ErhI CCWWGG 1 cut(s) 436
Esp3I CGTCTC 1 cut(s) 976
FaeI CATG 8 cut(s) 190, 254, 260, 401, 434, 460, 612, 621
FaqI GGGAC 1 cut(s) 245
FatI CATG 8 cut(s) 186, 250, 256, 397, 430, 456, 608, 617
FauI CCCGC 1 cut(s) 889
FblI GTMKAC 2 cut(s) 117, 886
Fnu4HI GCNGC 3 cut(s) 402, 725, 894
FokI GGATG 2 cut(s) 215, 794
FriOI GRGCYC 1 cut(s) 44
Fsp4HI GCNGC 3 cut(s) 402, 725, 894
FspBI CTAG 1 cut(s) 1077
GluI GCNGC 3 cut(s) 402, 725, 894
HaeIII GGCC 2 cut(s) 358, 1110
HapII CCGG 2 cut(s) 44, 675
Hin1II CATG 8 cut(s) 190, 254, 260, 401, 434, 460, 612, 621
HincII GTYRAC 2 cut(s) 61, 118
HindII GTYRAC 2 cut(s) 61, 118
HindIII AAGCTT 1 cut(s) 758
HinfI GANTC 4 cut(s) 101, 122, 290, 614
HpaII CCGG 2 cut(s) 44, 675
HphI GGTGA 1 cut(s) 1063
Hpy166II GTNNAC 3 cut(s) 61, 118, 887
Hpy188I TCNGA 2 cut(s) 337, 812
Hpy188III TCNNGA 6 cut(s) 160, 421, 751, 789, 824, 1039
Hpy8I GTNNAC 3 cut(s) 61, 118, 887
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 4 cut(s) 8, 188, 507, 528
HpyCH4III ACNGT 1 cut(s) 605
HpyCH4V TGCA 8 cut(s) 83, 113, 256, 401, 533, 608, 861, 913
HpyF10VI GCNNNNNNNGC 2 cut(s) 89, 858
HpyF3I CTNAG 2 cut(s) 579, 1113
Hsp92II CATG 8 cut(s) 190, 254, 260, 401, 434, 460, 612, 621
KpnI GGTACC 1 cut(s) 1069
Kzo9I GATC 4 cut(s) 423, 554, 655, 924
LguI GCTCTTC 1 cut(s) 471
LmnI GCTCC 1 cut(s) 624
Lsp1109I GCAGC 3 cut(s) 413, 711, 880
LweI GCATC 3 cut(s) 341, 711, 816
MaeI CTAG 1 cut(s) 1077
MaeIII GTNAC 4 cut(s) 282, 766, 899, 994
MalI GATC 4 cut(s) 425, 556, 657, 926
MboI GATC 4 cut(s) 423, 554, 655, 924
MflI RGATCY 1 cut(s) 423
MhlI GDGCHC 4 cut(s) 44, 466, 641, 981
MluCI AATT 7 cut(s) 53, 84, 738, 954, 1100, 1119, 1123
MmeI TCCRAC 1 cut(s) 602
MnlI CCTC 7 cut(s) 11, 167, 219, 727, 1079, 1100, 1108
MroXI GAANNNNTTC 1 cut(s) 1054
MseI TTAA 5 cut(s) 144, 165, 741, 1122, 1129
MslI CAYNNNNRTG 3 cut(s) 255, 278, 812
MspA1I CMGCKG 1 cut(s) 404
MspI CCGG 2 cut(s) 44, 675
MspR9I CCNGG 3 cut(s) 44, 45, 1085
MvaI CCWGG 1 cut(s) 1085
MwoI GCNNNNNNNGC 2 cut(s) 89, 858
NciI CCSGG 2 cut(s) 44, 45
NdeII GATC 4 cut(s) 423, 554, 655, 924
NlaIII CATG 8 cut(s) 190, 254, 260, 401, 434, 460, 612, 621
NlaIV GGNNCC 3 cut(s) 978, 1067, 1082
NmuCI GTSAC 2 cut(s) 282, 766
NspI RCATGY 1 cut(s) 260
PciSI GCTCTTC 1 cut(s) 471
PdmI GAANNNNTTC 1 cut(s) 1054
PfeI GAWTC 4 cut(s) 101, 122, 290, 614
PfoI TCCNGGA 1 cut(s) 1083
PkrI GCNGC 3 cut(s) 403, 726, 895
PshAI GACNNNNGTC 1 cut(s) 703
PshBI ATTAAT 2 cut(s) 165, 1122
Psp6I CCWGG 1 cut(s) 1083
PspGI CCWGG 1 cut(s) 1083
PspN4I GGNNCC 3 cut(s) 978, 1067, 1082
PspPI GGNCC 2 cut(s) 672, 1109
PsuI RGATCY 1 cut(s) 423
PvuII CAGCTG 1 cut(s) 404
RsaI GTAC 4 cut(s) 317, 386, 818, 1067
RsaNI GTAC 4 cut(s) 316, 385, 817, 1066
RseI CAYNNNNRTG 3 cut(s) 255, 278, 812
SalI GTCGAC 1 cut(s) 116
SapI GCTCTTC 1 cut(s) 471
SaqAI TTAA 5 cut(s) 144, 165, 741, 1122, 1129
SatI GCNGC 3 cut(s) 402, 725, 894
Sau3AI GATC 4 cut(s) 423, 554, 655, 924
Sau96I GGNCC 2 cut(s) 672, 1109
ScrFI CCNGG 3 cut(s) 44, 45, 1085
SduI GDGCHC 4 cut(s) 44, 466, 641, 981
SfaNI GCATC 3 cut(s) 341, 711, 816
SinI GGWCC 1 cut(s) 672
SmaI CCCGGG 1 cut(s) 45
SmiMI CAYNNNNRTG 3 cut(s) 255, 278, 812
Sse9I AATT 7 cut(s) 53, 84, 738, 954, 1100, 1119, 1123
SsiI CCGC 2 cut(s) 864, 896
SspI AATATT 1 cut(s) 417
SspMI CTAG 1 cut(s) 1077
StyD4I CCNGG 3 cut(s) 42, 43, 1083
StyI CCWWGG 1 cut(s) 436
TaaI ACNGT 1 cut(s) 605
TaqI TCGA 6 cut(s) 117, 553, 658, 697, 713, 927
TasI AATT 7 cut(s) 53, 84, 738, 954, 1100, 1119, 1123
TatI WGTACW 2 cut(s) 315, 816
TfiI GAWTC 4 cut(s) 101, 122, 290, 614
Tru1I TTAA 5 cut(s) 144, 165, 741, 1122, 1129
Tru9I TTAA 5 cut(s) 144, 165, 741, 1122, 1129
TscAI CASTG 5 cut(s) 42, 224, 280, 915, 1011
TseFI GTSAC 2 cut(s) 282, 766
TseI GCWGC 3 cut(s) 401, 724, 893
Tsp45I GTSAC 2 cut(s) 282, 766
TspDTI ATGAA 6 cut(s) 219, 362, 555, 606, 852, 1047
TspGWI ACGGA 1 cut(s) 697
TspMI CCCGGG 1 cut(s) 43
TspRI CASTG 5 cut(s) 42, 224, 280, 915, 1011
VpaK11BI GGWCC 1 cut(s) 672
VspI ATTAAT 2 cut(s) 165, 1122
XapI RAATTY 1 cut(s) 1100
XceI RCATGY 1 cut(s) 260
XcmI CCANNNNNNNNNTGG 2 cut(s) 434, 1081
XmaI CCCGGG 1 cut(s) 43
XmiI GTMKAC 2 cut(s) 117, 886
XmnI GAANNNNTTC 1 cut(s) 1054
XspI CTAG 1 cut(s) 1077
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.