MD05G1036400.v1.1

O-acyltransferase (WSD1-like)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000634
Physical Location & Seq
Forward (+)
5823990 .. 5826305
2316 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1036400.v1.1.491

Sequence Viewer

Length: 1452 bp
ATGGAGCACTTGGAGGAAGATGAAGTAGTAGTACTGGAGCCAGTGAGCCCTACTGCTCAGTACTTCACTAGCTCTGTCCTGTCCGTATCAAATATTGGCGTTATGGAACTCGAAAATCCACTGAGTGAGTCTCAAGCAATCTCATTACTGAAGACTCTCTTCCTCCCCATCAGCCCGCGCTTCTCCTCTATTGTGGTTGTAATCAATGGAAAGAAACGGTGGAAAAGGGTTGAAGTGAAGCCTGAAGAGCACATTAGCGTCCCTATTATTCCTTCTAACTTATCAACTGAATCATATGAAAAGTATCTTGATGGCTACATATCAAAGCTAGCAGTAGAAAGATTATCAGAAGACAAACCCTTATGGGAACTTCATATCCTCAATTACCCAACAAGAAATAATAACGCTGCTTGCAACCTTATATTCAAGCTCCATCATGCCCTCGGTGATGGCTTCTCTCTCATGAGTGCCATTCTTTCTTGTCTACGGAGAGCCGACAACCCTTCTCTTCCCCTAACATTTCCTTCAAGGCAGAGGTCACAGCAAACGAGTGATGAAAGCTTTGTGTCTAGAACTATCTCATCAGTCTTCAACACTCTTTCAGATATTTGGAGGAGCACCGTGGGGGAAGATGATCTATCACCAATTAGGTCTGGCAATGGAATTGAGTTTCAGCCCATTACATTGGCAACTATGACTTTCTCTCTTGACCAAATCAAATCAATCAAGAGTAAGCTTGGAGTGACGGTAAACGATGTTCTTGCCGGTATGATCTTCCTTGGCACTCGACTATACATGCAAGAGATAAACAGAAGTTCAAGCAAAGCAAGAGGCACGGCAGTGGTACTGCTGAACACGAGAATGATGGGGAAGTATACTTCAATCCAGGAGATGATGAAACCCGACAGCAAGATGCCATGGGGGAATCATTTTACGTTCTTGCATGTACCAATCCCCAATTTATCATTACCCGATGATCACGATCATGATTTTCCCGCTGATCAATATTCAAATGCCCTTGACTTTGTCTGGGAAGCACAAAAAATAATTACTAGGAAGAGAAGTTCTTTAGCTATTTATCTCACTTGTAGGTTCTTGGAGTTCCTCAACAAATTTGGAGGCCATGAGGCAGCAGCCAGATACATCCACAGCACACTGAAGAACACGAGCATGATGATCTCAAATTTGATTGGCCCTGTGGAACAAATGGCTTTAGCTAATAATCCAGTTAACGGCATGTACTTTCTGGTGTTTGGTTCACCTGAGGGGCTTGACGTAACAATAGTAAGTTATATGGGAAAGGTGAGGGTTGCCTTCAAAATGGAAAAGGGCCTCATTGAACCACAAAAGTTCAAGTCATGCATGCAAAATGCATTTGAGATGATACGCAAAGCCTCCGACGAATATCCTATTCCTATGCAGAAAAACTCAAAAACAATAAAACATCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1298 WES_acyltransf

Protein Analysis

484

Amino Acids

54.46

Weight (kDa)

7.71

Isoelectric Point (pI)

48.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WS_DGAT_cat PF03007 91 - 253 8.7e-11 Wax ester synthase/diacylglycerol acyltransferase catalytic domain
WS_DGAT_C PF06974 307 - 462 6.2e-36 WS/DGAT C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000634)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10290 FvH4_3g28230 FvH4_3g28240
malus_domestica MD05G1036400.v1.1 MD10G1159400.v1.1 MD17G1237100.v1.1 MD17G1237200.v1.1 MD17G1237300.v1.1 MD17G1237600.v1.1 MD17G1238200.v1.1
prunus_persica Prupe.3G140400_v2.0.a1 Prupe.3G140700_v2.0.a1 Prupe.3G141100_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G258100_v2.0.a1 Prupe.7G117300_v2.0.a1 Prupe.7G117400_v2.0.a1 Prupe.7G117600_v2.0.a1 Prupe.8G044700_v2.0.a1
pyrus_communis pycom05g02680 pycom17g24220 pycom17g24240
rosa_chinensis RchiOBHm_Chr1g0350141 RchiOBHm_Chr5g0050931 RchiOBHm_Chr5g0051001 RchiOBHm_Chr5g0051021 RchiOBHm_Chr6g0267491
rosa_laevigata RLG00000013999 RLG00000028502 RLG00000034744 RLG00000034748 RLG00000034753
rosa_multiflora Rmu_sc0000446.1_g000056 Rmu_sc0000782.1_g000006 Rmu_sc0002951.1_g000001 Rmu_sc0003674.1_g000026 Rmu_sc0003756.1_g000004 Rmu_sc0011917.1_g000004 Rmu_sc0012005.1_g000019 Rmu_sc0040739.1_g000001
rosa_roxburghii Rroxscaffold_1G00029730 Rroxscaffold_1G00029740 Rroxscaffold_4G00305060 Rroxscaffold_7G00200710
rosa_rugosa Rorug01G0208400 Rorug05G0265200.1 Rorug05G0265300.1 Rorug05G0265400.1 Rorug05G0265500.1 Rorug05G0294000 Rorug06G0033100 Rorug06G0243300
rosa_samantha Rh1BG192100 Rh1BG192200 Rh1DG220600 Rh5AG270300 Rh5AG337200 Rh5AG337700 Rh5BG346700 Rh5BG347400 Rh5CG374300 Rh5DG359700 Rh5DG360500 Rh6AG154500 Rh6BG155100 Rh6BG362300 Rh6CG150300 Rh6DG140300 Rh6DG356300
rosa_wichuraiana Rw0G018820 Rw1G019350 Rw5G025300 Rw5G031770 Rw5G031820 Rw5G031870 Rw5G031910 Rw6G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 484, 875
AccII CGCG 1 cut(s) 178
AciI CCGC 2 cut(s) 176, 996
AcsI RAATTY 2 cut(s) 1112, 1183
AcuI CTGAAG 3 cut(s) 170, 264, 1178
AdeI CACNNNGTG 1 cut(s) 125
AfaI GTAC 5 cut(s) 33, 62, 846, 948, 1241
AfiI CCNNNNNNNGG 1 cut(s) 1232
AjnI CCWGG 1 cut(s) 885
AleI CACNNNNGTG 1 cut(s) 839
AloI GAACNNNNNNTCC 4 cut(s) 360, 392, 1048, 1080
AluBI AGCT 7 cut(s) 72, 328, 430, 561, 736, 1073, 1217
AluI AGCT 7 cut(s) 72, 328, 430, 561, 736, 1073, 1217
Alw21I GWGCWC 3 cut(s) 9, 252, 620
Alw26I GTCTC 1 cut(s) 135
AoxI GGCC 3 cut(s) 1120, 1192, 1330
ApeKI GCWGC 3 cut(s) 407, 1130, 1133
ApoI RAATTY 2 cut(s) 1112, 1183
AspLEI GCGC 1 cut(s) 180
AspS9I GGNCC 2 cut(s) 1193, 1330
AsuHPI GGTGA 4 cut(s) 458, 633, 1251, 1315
AsuNHI GCTAGC 1 cut(s) 328
AxyI CCTNAGG 1 cut(s) 1263
BanII GRGCYC 1 cut(s) 50
BarI GAAGNNNNNNTAC 2 cut(s) 15, 47
BauI CACGAG 2 cut(s) 856, 1165
BbsI GAAGAC 3 cut(s) 158, 357, 580
Bbv12I GWGCWC 3 cut(s) 9, 252, 620
BbvI GCAGC 3 cut(s) 394, 1142, 1145
BccI CCATC 5 cut(s) 176, 305, 441, 443, 859
BceAI ACGGC 2 cut(s) 852, 1249
BcgI CGANNNNNNTGC 2 cut(s) 743, 777
BciT130I CCWGG 1 cut(s) 887
BclI TGATCA 2 cut(s) 976, 1000
BcoDI GTCTC 1 cut(s) 135
BfaI CTAG 4 cut(s) 69, 329, 570, 1053
BisI GCNGC 3 cut(s) 408, 1131, 1134
BlsI GCNGC 3 cut(s) 409, 1132, 1135
BmcAI AGTACT 2 cut(s) 33, 62
Bme1390I CCNGG 1 cut(s) 887
BmgT120I GGNCC 2 cut(s) 1193, 1330
BmiI GGNNCC 1 cut(s) 39
BmrFI CCNGG 1 cut(s) 887
BmsI GCATC 1 cut(s) 903
BmtI GCTAGC 1 cut(s) 332
BpiI GAAGAC 3 cut(s) 158, 357, 580
BplI GAGNNNNNCTC 2 cut(s) 115, 147
BpmI CTGGAG 1 cut(s) 56
BpuEI CTTGAG 1 cut(s) 117
BsaBI GATNNNNATC 1 cut(s) 981
BsaJI CCNNGG 4 cut(s) 442, 621, 778, 917
BsaXI ACNNNNNCTCC 2 cut(s) 732, 762
Bsc4I CCNNNNNNNGG 1 cut(s) 1232
Bse118I RCCGGY 1 cut(s) 764
Bse1I ACTGG 3 cut(s) 39, 41, 1226
Bse21I CCTNAGG 1 cut(s) 1263
Bse3DI GCAATG 1 cut(s) 664
Bse8I GATNNNNATC 1 cut(s) 981
BseBI CCWGG 1 cut(s) 887
BseDI CCNNGG 4 cut(s) 442, 621, 778, 917
BseGI GGATG 1 cut(s) 1143
BseJI GATNNNNATC 1 cut(s) 981
BseLI CCNNNNNNNGG 1 cut(s) 1232
BseMI GCAATG 1 cut(s) 664
BseMII CTCAG 3 cut(s) 71, 113, 1254
BseNI ACTGG 3 cut(s) 39, 41, 1226
BseRI GAGGAG 2 cut(s) 175, 628
BseXI GCAGC 3 cut(s) 394, 1142, 1145
Bsh1236I CGCG 1 cut(s) 178
BshFI GGCC 3 cut(s) 1122, 1194, 1332
BsiHKAI GWGCWC 3 cut(s) 9, 252, 620
BsiSI CCGG 1 cut(s) 765
BslFI GGGAC 1 cut(s) 245
BslI CCNNNNNNNGG 1 cut(s) 1232
BsmAI GTCTC 1 cut(s) 135
BsmFI GGGAC 1 cut(s) 245
BsnI GGCC 3 cut(s) 1122, 1194, 1332
Bsp1286I GDGCHC 4 cut(s) 9, 50, 252, 620
Bsp143I GATC 6 cut(s) 634, 771, 976, 982, 1000, 1176
Bsp19I CCATGG 1 cut(s) 917
BspACI CCGC 2 cut(s) 176, 996
BspANI GGCC 3 cut(s) 1122, 1194, 1332
BspCNI CTCAG 3 cut(s) 70, 114, 1255
BspFNI CGCG 1 cut(s) 178
BspHI TCATGA 2 cut(s) 462, 985
BspLI GGNNCC 1 cut(s) 39
BspOI GCTAGC 1 cut(s) 332
BspQI GCTCTTC 1 cut(s) 240
BsrDI GCAATG 1 cut(s) 664
BsrFI RCCGGY 1 cut(s) 764
BsrI ACTGG 3 cut(s) 39, 41, 1226
BssAI RCCGGY 1 cut(s) 764
BssECI CCNNGG 4 cut(s) 442, 621, 778, 917
BssMI GATC 6 cut(s) 634, 771, 976, 982, 1000, 1176
BssNAI GTATAC 1 cut(s) 876
BssSI CACGAG 2 cut(s) 856, 1165
BssT1I CCWWGG 2 cut(s) 778, 917
Bst1107I GTATAC 1 cut(s) 876
Bst2BI CACGAG 2 cut(s) 856, 1165
Bst2UI CCWGG 1 cut(s) 887
Bst4CI ACNGT 3 cut(s) 219, 622, 748
Bst6I CTCTTC 4 cut(s) 164, 240, 513, 1052
BstC8I GCNNGC 4 cut(s) 176, 330, 412, 1364
BstDEI CTNAG 3 cut(s) 57, 122, 1263
BstDSI CCRYGG 2 cut(s) 621, 917
BstF5I GGATG 1 cut(s) 1143
BstFNI CGCG 1 cut(s) 178
BstHHI GCGC 1 cut(s) 180
BstKTI GATC 6 cut(s) 637, 774, 979, 985, 1003, 1179
BstMAI GTCTC 1 cut(s) 135
BstMBI GATC 6 cut(s) 634, 771, 976, 982, 1000, 1176
BstMWI GCNNNNNNNGC 1 cut(s) 247
BstNI CCWGG 1 cut(s) 887
BstNSI RCATGY 4 cut(s) 799, 947, 1240, 1366
BstSCI CCNGG 1 cut(s) 885
BstUI CGCG 1 cut(s) 178
BstV1I GCAGC 3 cut(s) 394, 1142, 1145
BstV2I GAAGAC 3 cut(s) 158, 357, 580
BstXI CCANNNNNNTGG 1 cut(s) 685
BstZ17I GTATAC 1 cut(s) 876
Bsu36I CCTNAGG 1 cut(s) 1263
BsuRI GGCC 3 cut(s) 1122, 1194, 1332
BtgI CCRYGG 2 cut(s) 621, 917
BtsCI GGATG 1 cut(s) 1143
BtsI GCAGTG 1 cut(s) 846
BtsIMutI CAGTG 4 cut(s) 48, 119, 846, 1154
Cac8I GCNNGC 4 cut(s) 176, 330, 412, 1364
CciI TCATGA 2 cut(s) 462, 985
CfoI GCGC 1 cut(s) 180
Cfr10I RCCGGY 1 cut(s) 764
Cfr13I GGNCC 2 cut(s) 1193, 1330
CseI GACGC 1 cut(s) 247
Csp6I GTAC 5 cut(s) 32, 61, 845, 947, 1240
CviQI GTAC 5 cut(s) 32, 61, 845, 947, 1240
DdeI CTNAG 3 cut(s) 57, 122, 1263
DpnI GATC 6 cut(s) 636, 773, 978, 984, 1002, 1178
DpnII GATC 6 cut(s) 634, 771, 976, 982, 1000, 1176
DraIII CACNNNGTG 1 cut(s) 125
Eam1104I CTCTTC 4 cut(s) 164, 240, 513, 1052
EarI CTCTTC 4 cut(s) 164, 240, 513, 1052
Eco130I CCWWGG 2 cut(s) 778, 917
Eco24I GRGCYC 1 cut(s) 50
Eco57I CTGAAG 3 cut(s) 170, 264, 1178
Eco81I CCTNAGG 1 cut(s) 1263
EcoO109I RGGNCCY 1 cut(s) 1330
EcoRII CCWGG 1 cut(s) 885
EcoT14I CCWWGG 2 cut(s) 778, 917
EcoT22I ATGCAT 2 cut(s) 1364, 1375
EcoT38I GRGCYC 1 cut(s) 50
ErhI CCWWGG 2 cut(s) 778, 917
FalI AAGNNNNNCTT 2 cut(s) 143, 175
FaqI GGGAC 1 cut(s) 245
FauI CCCGC 2 cut(s) 183, 1003
FauNDI CATATG 1 cut(s) 295
FbaI TGATCA 2 cut(s) 976, 1000
FblI GTMKAC 2 cut(s) 484, 875
Fnu4HI GCNGC 3 cut(s) 408, 1131, 1134
FokI GGATG 1 cut(s) 1130
FriOI GRGCYC 1 cut(s) 50
Fsp4HI GCNGC 3 cut(s) 408, 1131, 1134
FspBI CTAG 4 cut(s) 69, 329, 570, 1053
GlaI GCGC 1 cut(s) 179
GluI GCNGC 3 cut(s) 408, 1131, 1134
GsuI CTGGAG 1 cut(s) 56
HaeIII GGCC 3 cut(s) 1122, 1194, 1332
HapII CCGG 1 cut(s) 765
HgaI GACGC 1 cut(s) 247
HhaI GCGC 1 cut(s) 180
Hin6I GCGC 1 cut(s) 178
HinP1I GCGC 1 cut(s) 178
HincII GTYRAC 1 cut(s) 1231
HindII GTYRAC 1 cut(s) 1231
HindIII AAGCTT 2 cut(s) 559, 734
HinfI GANTC 4 cut(s) 128, 154, 290, 925
HpaI GTTAAC 1 cut(s) 1231
HpaII CCGG 1 cut(s) 765
HphI GGTGA 4 cut(s) 458, 633, 1251, 1315
Hpy166II GTNNAC 5 cut(s) 485, 751, 876, 1231, 1259
Hpy188I TCNGA 3 cut(s) 349, 604, 1399
Hpy188III TCNNGA 7 cut(s) 308, 463, 570, 707, 727, 980, 986
Hpy8I GTNNAC 5 cut(s) 485, 751, 876, 1231, 1259
Hpy99I CGWCG 1 cut(s) 1403
HpyAV CCTTC 4 cut(s) 282, 513, 534, 1324
HpyCH4III ACNGT 3 cut(s) 219, 622, 748
HpyCH4IV ACGT 2 cut(s) 935, 1275
HpyCH4V TGCA 7 cut(s) 414, 799, 943, 1362, 1366, 1373, 1420
HpyF10VI GCNNNNNNNGC 1 cut(s) 247
HpyF3I CTNAG 3 cut(s) 57, 122, 1263
HpySE526I ACGT 2 cut(s) 935, 1275
HspAI GCGC 1 cut(s) 178
Ksp22I TGATCA 2 cut(s) 976, 1000
KspAI GTTAAC 1 cut(s) 1231
Kzo9I GATC 6 cut(s) 634, 771, 976, 982, 1000, 1176
LguI GCTCTTC 1 cut(s) 240
LmnI GCTCC 4 cut(s) 4, 37, 435, 615
Lsp1109I GCAGC 3 cut(s) 394, 1142, 1145
LweI GCATC 1 cut(s) 903
MaeI CTAG 4 cut(s) 69, 329, 570, 1053
MaeII ACGT 2 cut(s) 935, 1275
MaeIII GTNAC 3 cut(s) 537, 742, 1276
MalI GATC 6 cut(s) 636, 773, 978, 984, 1002, 1178
MboI GATC 6 cut(s) 634, 771, 976, 982, 1000, 1176
MhlI GDGCHC 4 cut(s) 9, 50, 252, 620
MluCI AATT 7 cut(s) 382, 645, 663, 958, 1047, 1112, 1183
MlyI GAGTC 2 cut(s) 137, 148
MmeI TCCRAC 1 cut(s) 1422
Mph1103I ATGCAT 2 cut(s) 1364, 1375
MseI TTAA 2 cut(s) 1230, 1450
MslI CAYNNNNRTG 4 cut(s) 839, 860, 984, 1169
MspA1I CMGCKG 1 cut(s) 998
MspI CCGG 1 cut(s) 765
MspR9I CCNGG 1 cut(s) 887
MvaI CCWGG 1 cut(s) 887
MvnI CGCG 1 cut(s) 178
MwoI GCNNNNNNNGC 1 cut(s) 247
NcoI CCATGG 1 cut(s) 917
NdeI CATATG 1 cut(s) 295
NdeII GATC 6 cut(s) 634, 771, 976, 982, 1000, 1176
NheI GCTAGC 1 cut(s) 328
NlaIV GGNNCC 1 cut(s) 39
NmuCI GTSAC 2 cut(s) 537, 742
NsiI ATGCAT 2 cut(s) 1364, 1375
NspI RCATGY 4 cut(s) 799, 947, 1240, 1366
OliI CACNNNNGTG 1 cut(s) 839
PaeI GCATGC 1 cut(s) 1366
PagI TCATGA 2 cut(s) 462, 985
PciSI GCTCTTC 1 cut(s) 240
PfeI GAWTC 2 cut(s) 290, 925
PflFI GACNNNGTC 1 cut(s) 1025
PfoI TCCNGGA 1 cut(s) 885
PkrI GCNGC 3 cut(s) 409, 1132, 1135
PleI GAGTC 2 cut(s) 136, 148
PpsI GAGTC 2 cut(s) 136, 148
Psp6I CCWGG 1 cut(s) 885
PspGI CCWGG 1 cut(s) 885
PspN4I GGNNCC 1 cut(s) 39
PspPI GGNCC 2 cut(s) 1193, 1330
PsrI GAACNNNNNNTAC 2 cut(s) 741, 773
PsyI GACNNNGTC 1 cut(s) 1025
RsaI GTAC 5 cut(s) 33, 62, 846, 948, 1241
RsaNI GTAC 5 cut(s) 32, 61, 845, 947, 1240
RseI CAYNNNNRTG 4 cut(s) 839, 860, 984, 1169
SapI GCTCTTC 1 cut(s) 240
SaqAI TTAA 2 cut(s) 1230, 1450
SatI GCNGC 3 cut(s) 408, 1131, 1134
Sau3AI GATC 6 cut(s) 634, 771, 976, 982, 1000, 1176
Sau96I GGNCC 2 cut(s) 1193, 1330
ScaI AGTACT 2 cut(s) 33, 62
SchI GAGTC 2 cut(s) 137, 148
ScrFI CCNGG 1 cut(s) 887
SduI GDGCHC 4 cut(s) 9, 50, 252, 620
SfaNI GCATC 1 cut(s) 903
SmiMI CAYNNNNRTG 4 cut(s) 839, 860, 984, 1169
SmlI CTYRAG 1 cut(s) 132
SmoI CTYRAG 1 cut(s) 132
SphI GCATGC 1 cut(s) 1366
Sse9I AATT 7 cut(s) 382, 645, 663, 958, 1047, 1112, 1183
SsiI CCGC 2 cut(s) 176, 996
SspI AATATT 2 cut(s) 94, 1007
SspMI CTAG 4 cut(s) 69, 329, 570, 1053
StyD4I CCNGG 1 cut(s) 885
StyI CCWWGG 2 cut(s) 778, 917
TaaI ACNGT 3 cut(s) 219, 622, 748
TaiI ACGT 2 cut(s) 938, 1278
TaqI TCGA 2 cut(s) 111, 787
TasI AATT 7 cut(s) 382, 645, 663, 958, 1047, 1112, 1183
TatI WGTACW 3 cut(s) 31, 60, 1239
TfiI GAWTC 2 cut(s) 290, 925
Tru1I TTAA 2 cut(s) 1230, 1450
Tru9I TTAA 2 cut(s) 1230, 1450
TscAI CASTG 4 cut(s) 48, 126, 846, 1161
TseFI GTSAC 2 cut(s) 537, 742
TseI GCWGC 3 cut(s) 407, 1130, 1133
Tsp45I GTSAC 2 cut(s) 537, 742
TspDTI ATGAA 5 cut(s) 36, 312, 362, 570, 911
TspGWI ACGGA 2 cut(s) 73, 502
TspRI CASTG 4 cut(s) 48, 126, 846, 1161
Tth111I GACNNNGTC 1 cut(s) 1025
XapI RAATTY 2 cut(s) 1112, 1183
XbaI TCTAGA 1 cut(s) 569
XceI RCATGY 4 cut(s) 799, 947, 1240, 1366
XmiI GTMKAC 2 cut(s) 484, 875
XspI CTAG 4 cut(s) 69, 329, 570, 1053
ZrmI AGTACT 2 cut(s) 33, 62
Zsp2I ATGCAT 2 cut(s) 1364, 1375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.