Rh6BG155100

O-acyltransferase (WSD1-like)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000634
Physical Location & Seq
Forward (+)
25111425 .. 25125791
14367 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG155100.1

Sequence Viewer

Length: 879 bp
ATGGGTGCTCTTCTCTCTTGTTTACAACGAGCTGACAATCCTTCTCTTCCCCTAACCTTCCCGTCAAGAAGGAGGTCGCAGCCATGGAAGAGTGAAAATTTTGTGACTAAAGTTTTTTCTTCTGTCTTCAACACCATATCGGATTTTTTAAATAGCAACTTGGAGGAAGATGATCAAACACCAATCAGATCGGGGAATGATGGAATTGAGTTCAAGCCAATTGCAGTATCAACTATGACGATTTCCCTTGATAGAATCAAACTAATCAAGAGCAGGGTCGGAGTGACGATAAATGACGTTCTTACCGCGATGATCTTCCTTGGCACTCGACTATACATCCAAGAGACAAACAAAAGTTTAAGCAAAGCAAGAGGCACAGCAATTGTATTGCTCAACACAAGGATGATGGGGAATTACACTTCAGTTCAGGAGATGATCAAACCTAATAGCAAGTTGCCATGGGGGAACCATTTGTCACTGTTGCATGTACCAATACCCAAGTTGTTAACGAATGGTGAAGAACAATATTTTTCAGATAATGCACTTGACTTTGTCTGGAAGACACAGAAAATTTTAAGGAGCAAAAGACATTCTTTGGGTATTCATCTCACTGCTGGGTTCTTGGAGATTCTCAACAAATTTGGAGGCCATGAGGCAGCAGCAAAATACATCCGTAACACATTGAAGAAGTCGAGCATGATAATTTCCAATATGATTGGACCGGTGGAACAGATGTCTTTGGCAAATCATCCAGTTAAAGGCTTATACTTTTTGGTCTTTGGTTCACCTGAGGAAGAAAATTCTGATTTTCCGTCACCCAGATCCTTCAATTTTGGAGGGTTTTCATTCATAGCTCACAAGGAACTAGAAAACGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1298 WES_acyltransf

Protein Analysis

292

Amino Acids

32.74

Weight (kDa)

9.45

Isoelectric Point (pI)

42.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WS_DGAT_C PF06974 154 - 264 3.5e-23 WS/DGAT C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000634)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10290 FvH4_3g28230 FvH4_3g28240
malus_domestica MD05G1036400.v1.1 MD10G1159400.v1.1 MD17G1237100.v1.1 MD17G1237200.v1.1 MD17G1237300.v1.1 MD17G1237600.v1.1 MD17G1238200.v1.1
prunus_persica Prupe.3G140400_v2.0.a1 Prupe.3G140700_v2.0.a1 Prupe.3G141100_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G258100_v2.0.a1 Prupe.7G117300_v2.0.a1 Prupe.7G117400_v2.0.a1 Prupe.7G117600_v2.0.a1 Prupe.8G044700_v2.0.a1
pyrus_communis pycom05g02680 pycom17g24220 pycom17g24240
rosa_chinensis RchiOBHm_Chr1g0350141 RchiOBHm_Chr5g0050931 RchiOBHm_Chr5g0051001 RchiOBHm_Chr5g0051021 RchiOBHm_Chr6g0267491
rosa_laevigata RLG00000013999 RLG00000028502 RLG00000034744 RLG00000034748 RLG00000034753
rosa_multiflora Rmu_sc0000446.1_g000056 Rmu_sc0000782.1_g000006 Rmu_sc0002951.1_g000001 Rmu_sc0003674.1_g000026 Rmu_sc0003756.1_g000004 Rmu_sc0011917.1_g000004 Rmu_sc0012005.1_g000019 Rmu_sc0040739.1_g000001
rosa_roxburghii Rroxscaffold_1G00029730 Rroxscaffold_1G00029740 Rroxscaffold_4G00305060 Rroxscaffold_7G00200710
rosa_rugosa Rorug01G0208400 Rorug05G0265200.1 Rorug05G0265300.1 Rorug05G0265400.1 Rorug05G0265500.1 Rorug05G0294000 Rorug06G0033100 Rorug06G0243300
rosa_samantha Rh1BG192100 Rh1BG192200 Rh1DG220600 Rh5AG270300 Rh5AG337200 Rh5AG337700 Rh5BG346700 Rh5BG347400 Rh5CG374300 Rh5DG359700 Rh5DG360500 Rh6AG154500 Rh6BG155100 Rh6BG362300 Rh6CG150300 Rh6DG140300 Rh6DG356300
rosa_wichuraiana Rw0G018820 Rw1G019350 Rw5G025300 Rw5G031770 Rw5G031820 Rw5G031870 Rw5G031910 Rw6G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 308
AciI CCGC 1 cut(s) 306
AclWI GGATC 1 cut(s) 816
AcsI RAATTY 4 cut(s) 97, 570, 638, 799
AcuI CTGAAG 1 cut(s) 405
AfaI GTAC 1 cut(s) 489
AfiI CCNNNNNNNGG 1 cut(s) 758
AgeI ACCGGT 1 cut(s) 721
AgsI TTSAA 4 cut(s) 130, 214, 685, 829
AluBI AGCT 2 cut(s) 32, 854
AluI AGCT 2 cut(s) 32, 854
Alw21I GWGCWC 1 cut(s) 10
Alw26I GTCTC 1 cut(s) 338
AlwI GGATC 1 cut(s) 816
AoxI GGCC 1 cut(s) 646
ApeKI GCWGC 3 cut(s) 79, 656, 659
ApoI RAATTY 4 cut(s) 97, 570, 638, 799
AsiGI ACCGGT 1 cut(s) 721
AspS9I GGNCC 1 cut(s) 719
AsuHPI GGTGA 3 cut(s) 527, 777, 807
AvaII GGWCC 1 cut(s) 719
AxyI CCTNAGG 1 cut(s) 789
BbsI GAAGAC 2 cut(s) 118, 566
Bbv12I GWGCWC 1 cut(s) 10
BbvI GCAGC 3 cut(s) 91, 668, 671
BccI CCATC 2 cut(s) 194, 400
BclI TGATCA 2 cut(s) 172, 435
BcoDI GTCTC 1 cut(s) 338
BfaI CTAG 1 cut(s) 866
BisI GCNGC 3 cut(s) 80, 657, 660
BlsI GCNGC 3 cut(s) 81, 658, 661
Bme18I GGWCC 1 cut(s) 719
BmgT120I GGNCC 1 cut(s) 719
BmiI GGNNCC 1 cut(s) 467
BpiI GAAGAC 2 cut(s) 118, 566
BsaJI CCNNGG 3 cut(s) 83, 319, 458
BsaWI WCCGGW 1 cut(s) 721
Bsc4I CCNNNNNNNGG 1 cut(s) 758
Bse118I RCCGGY 1 cut(s) 721
Bse1I ACTGG 1 cut(s) 752
Bse21I CCTNAGG 1 cut(s) 789
BseDI CCNNGG 3 cut(s) 83, 319, 458
BseGI GGATG 4 cut(s) 336, 408, 669, 748
BseLI CCNNNNNNNGG 1 cut(s) 758
BseMII CTCAG 1 cut(s) 780
BseNI ACTGG 1 cut(s) 752
BseXI GCAGC 3 cut(s) 91, 668, 671
BseYI CCCAGC 1 cut(s) 614
Bsh1236I CGCG 1 cut(s) 308
BshFI GGCC 1 cut(s) 648
BshTI ACCGGT 1 cut(s) 721
BsiHKAI GWGCWC 1 cut(s) 10
BsiSI CCGG 1 cut(s) 722
BslI CCNNNNNNNGG 1 cut(s) 758
BsmAI GTCTC 1 cut(s) 338
BsnI GGCC 1 cut(s) 648
Bsp1286I GDGCHC 1 cut(s) 10
Bsp143I GATC 5 cut(s) 172, 188, 312, 435, 821
Bsp19I CCATGG 2 cut(s) 83, 458
BspACI CCGC 1 cut(s) 306
BspANI GGCC 1 cut(s) 648
BspCNI CTCAG 1 cut(s) 781
BspFNI CGCG 1 cut(s) 308
BspLI GGNNCC 1 cut(s) 467
BspPI GGATC 1 cut(s) 816
BspQI GCTCTTC 1 cut(s) 15
BsrFI RCCGGY 1 cut(s) 721
BsrI ACTGG 1 cut(s) 752
BssAI RCCGGY 1 cut(s) 721
BssECI CCNNGG 3 cut(s) 83, 319, 458
BssMI GATC 5 cut(s) 172, 188, 312, 435, 821
BssT1I CCWWGG 3 cut(s) 83, 319, 458
Bst4CI ACNGT 1 cut(s) 480
Bst6I CTCTTC 3 cut(s) 15, 51, 83
BstDEI CTNAG 1 cut(s) 789
BstDSI CCRYGG 2 cut(s) 83, 458
BstF5I GGATG 4 cut(s) 336, 408, 669, 748
BstFNI CGCG 1 cut(s) 308
BstKTI GATC 5 cut(s) 175, 191, 315, 438, 824
BstMAI GTCTC 1 cut(s) 338
BstMBI GATC 5 cut(s) 172, 188, 312, 435, 821
BstNSI RCATGY 1 cut(s) 488
BstUI CGCG 1 cut(s) 308
BstV1I GCAGC 3 cut(s) 91, 668, 671
BstV2I GAAGAC 2 cut(s) 118, 566
BstX2I RGATCY 1 cut(s) 821
BstYI RGATCY 1 cut(s) 821
Bsu36I CCTNAGG 1 cut(s) 789
BsuRI GGCC 1 cut(s) 648
BtgI CCRYGG 2 cut(s) 83, 458
BtgZI GCGATG 1 cut(s) 323
BtsCI GGATG 4 cut(s) 336, 408, 669, 748
BtsI GCAGTG 1 cut(s) 609
BtsIMutI CAGTG 2 cut(s) 476, 609
Cfr10I RCCGGY 1 cut(s) 721
Cfr13I GGNCC 1 cut(s) 719
Csp6I GTAC 1 cut(s) 488
CspAI ACCGGT 1 cut(s) 721
CviAII CATG 5 cut(s) 84, 459, 485, 650, 697
CviJI RGCY 6 cut(s) 32, 82, 217, 648, 762, 854
CviKI_1 RGCY 6 cut(s) 32, 82, 217, 648, 762, 854
CviQI GTAC 1 cut(s) 488
DdeI CTNAG 1 cut(s) 789
DpnI GATC 5 cut(s) 174, 190, 314, 437, 823
DpnII GATC 5 cut(s) 172, 188, 312, 435, 821
DraI TTTAAA 1 cut(s) 150
Eam1104I CTCTTC 3 cut(s) 15, 51, 83
EarI CTCTTC 3 cut(s) 15, 51, 83
Eco130I CCWWGG 3 cut(s) 83, 319, 458
Eco47I GGWCC 1 cut(s) 719
Eco57I CTGAAG 1 cut(s) 405
Eco81I CCTNAGG 1 cut(s) 789
EcoT14I CCWWGG 3 cut(s) 83, 319, 458
ErhI CCWWGG 3 cut(s) 83, 319, 458
FaeI CATG 5 cut(s) 87, 462, 488, 653, 700
FalI AAGNNNNNCTT 2 cut(s) 577, 609
FatI CATG 5 cut(s) 83, 458, 484, 649, 696
FbaI TGATCA 2 cut(s) 172, 435
Fnu4HI GCNGC 3 cut(s) 80, 657, 660
FokI GGATG 4 cut(s) 323, 415, 656, 735
Fsp4HI GCNGC 3 cut(s) 80, 657, 660
FspBI CTAG 1 cut(s) 866
GluI GCNGC 3 cut(s) 80, 657, 660
GsaI CCCAGC 1 cut(s) 618
HaeIII GGCC 1 cut(s) 648
HapII CCGG 1 cut(s) 722
Hin1II CATG 5 cut(s) 87, 462, 488, 653, 700
HincII GTYRAC 1 cut(s) 507
HindII GTYRAC 1 cut(s) 507
HinfI GANTC 2 cut(s) 255, 628
HpaI GTTAAC 1 cut(s) 507
HpaII CCGG 1 cut(s) 722
HphI GGTGA 3 cut(s) 527, 777, 807
Hpy166II GTNNAC 3 cut(s) 23, 507, 785
Hpy188I TCNGA 5 cut(s) 142, 188, 281, 535, 805
Hpy188III TCNNGA 4 cut(s) 66, 268, 428, 556
Hpy8I GTNNAC 3 cut(s) 23, 507, 785
HpyAV CCTTC 4 cut(s) 51, 63, 67, 835
HpyCH4III ACNGT 1 cut(s) 480
HpyCH4IV ACGT 1 cut(s) 297
HpyCH4V TGCA 3 cut(s) 224, 484, 542
HpyF3I CTNAG 1 cut(s) 789
HpySE526I ACGT 1 cut(s) 297
Hsp92II CATG 5 cut(s) 87, 462, 488, 653, 700
Ksp22I TGATCA 2 cut(s) 172, 435
KspAI GTTAAC 1 cut(s) 507
Kzo9I GATC 5 cut(s) 172, 188, 312, 435, 821
LguI GCTCTTC 1 cut(s) 15
LmnI GCTCC 1 cut(s) 579
LpnPI CCDG 8 cut(s) 259, 413, 541, 600, 735, 765, 801, 832
Lsp1109I GCAGC 3 cut(s) 91, 668, 671
MaeI CTAG 1 cut(s) 866
MaeII ACGT 1 cut(s) 297
MaeIII GTNAC 5 cut(s) 103, 283, 474, 674, 813
MalI GATC 5 cut(s) 174, 190, 314, 437, 823
MboI GATC 5 cut(s) 172, 188, 312, 435, 821
MfeI CAATTG 2 cut(s) 219, 381
MflI RGATCY 1 cut(s) 821
MhlI GDGCHC 1 cut(s) 10
MmeI TCCRAC 1 cut(s) 259
MnlI CCTC 7 cut(s) 66, 157, 365, 638, 646, 784, 830
MseI TTAA 5 cut(s) 149, 359, 506, 575, 756
MslI CAYNNNNRTG 1 cut(s) 401
MspI CCGG 1 cut(s) 722
MunI CAATTG 2 cut(s) 219, 381
MvnI CGCG 1 cut(s) 308
NcoI CCATGG 2 cut(s) 83, 458
NdeII GATC 5 cut(s) 172, 188, 312, 435, 821
NlaIII CATG 5 cut(s) 87, 462, 488, 653, 700
NlaIV GGNNCC 1 cut(s) 467
NmuCI GTSAC 4 cut(s) 103, 283, 474, 813
NspI RCATGY 1 cut(s) 488
PciSI GCTCTTC 1 cut(s) 15
PfeI GAWTC 2 cut(s) 255, 628
PflFI GACNNNGTC 1 cut(s) 551
PinAI ACCGGT 1 cut(s) 721
PkrI GCNGC 3 cut(s) 81, 658, 661
PspFI CCCAGC 1 cut(s) 614
PspN4I GGNNCC 1 cut(s) 467
PspPI GGNCC 1 cut(s) 719
PsuI RGATCY 1 cut(s) 821
PsyI GACNNNGTC 1 cut(s) 551
RsaI GTAC 1 cut(s) 489
RsaNI GTAC 1 cut(s) 488
RseI CAYNNNNRTG 1 cut(s) 401
SapI GCTCTTC 1 cut(s) 15
SaqAI TTAA 5 cut(s) 149, 359, 506, 575, 756
SatI GCNGC 3 cut(s) 80, 657, 660
Sau3AI GATC 5 cut(s) 172, 188, 312, 435, 821
Sau96I GGNCC 1 cut(s) 719
SduI GDGCHC 1 cut(s) 10
SetI ASST 7 cut(s) 34, 59, 77, 300, 445, 790, 856
SinI GGWCC 1 cut(s) 719
SmiMI CAYNNNNRTG 1 cut(s) 401
SsiI CCGC 1 cut(s) 306
SspI AATATT 1 cut(s) 527
SspMI CTAG 1 cut(s) 866
StyI CCWWGG 3 cut(s) 83, 319, 458
TaaI ACNGT 1 cut(s) 480
TaiI ACGT 1 cut(s) 300
TaqI TCGA 2 cut(s) 328, 692
TfiI GAWTC 2 cut(s) 255, 628
Tru1I TTAA 5 cut(s) 149, 359, 506, 575, 756
Tru9I TTAA 5 cut(s) 149, 359, 506, 575, 756
TscAI CASTG 2 cut(s) 483, 616
TseFI GTSAC 4 cut(s) 103, 283, 474, 813
TseI GCWGC 3 cut(s) 79, 656, 659
Tsp45I GTSAC 4 cut(s) 103, 283, 474, 813
TspDTI ATGAA 3 cut(s) 593, 834, 838
TspGWI ACGGA 2 cut(s) 662, 801
TspRI CASTG 2 cut(s) 483, 616
Tth111I GACNNNGTC 1 cut(s) 551
VpaK11BI GGWCC 1 cut(s) 719
XapI RAATTY 4 cut(s) 97, 570, 638, 799
XceI RCATGY 1 cut(s) 488
XspI CTAG 1 cut(s) 866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.