Rw0G018820

O-acyltransferase (WSD1-like)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00847
OG0000634
Physical Location & Seq
Reverse (-)
195444 .. 205367
9924 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G018820.1

Sequence Viewer

Length: 1344 bp
ATGGAGTCTGGAGAAGAAGGACTACTACTGGAGCCGGTGAGCCCTGGTGGTCAATTCTTCAACAGCACTGTCTTATCTCTTGCAATTATATCCGTTTTGGAATCACAAGTTCCAATAAAAGATTCTCAAACTTTTTCACTTCACAAAAATGTGTTCCTCCCGATCAATCCACGCTTCTCTTTCCATCATGTAACGCAGGTTGAAGGTGCGGATGAAAAGAATAAGCAGTGGAAGAGGGTTCAAGTGAAGCTGGAAGACCATGTGTATGTCCCCATTTTCCCCTCTAGAATGTCATTTGAATCATACGATACTTACTTTGATGAGTATATATATAACGAAGATAGCATCAGAAACAACCTGAAGGGTCTGAACCCCACGGATGTCAAATCCTGGTTCCGCCCCTGGTGGGAACTTCATCTTTTCAAGTACCCAACAAGTCATGCAGCTGGACAAATAATATTCAAGATCCACCATGGCCTTGGCGATGGCTACTCTCTTATGGGCGCTCATCTCTCTTGTCTCCAAAATGCTCACAATCCTTCTCTTCCCCTGACATTTCCTTCACTAAAGGGTGCAAAGAATGAAACTAGTAGTCTGTCTCGTGTGTTTGAGTTTGTGCCTAAGATACTTTCTGCCGTTATCAACGTTGCATGGGATTTCAGTTGGAGCATTTTGAAGGGCACTTGGGTTGAAGATGATCGAACACCAACAAGGTCCGGCGTTGTTGGAGTTGAGTTTCGGCCTGTGTCCATGTCGACCTTGATGCTGTCTATTGAAGAAATCAAAATTATTAAGAACAAGCTTGGGGTGACGATAAATGATGTTATTGCGGGAATAACCTTTCTGGGCACCCGAATGTACATACAACGGATGAATAGTGAAAAATCAAGTAGCCAGAATTGCACGGCACTCGTGTTGCTAAATACTAGACTTGCTGCGGGTTACAAGTCGGTGCAGGAGATGTTGATGGAACCAAGCAAGTCTTCTTGGGGCAACCGATTTGTGTTCTTGCATGTCTCGGTGCCCAAGTCGAGTGAAGTTTCCAAGCCATTGGATTTTGTATGGGAAGCACAGAAGATAGTCAAGAGACATAGAAGCTCTTCAGCTTGCACACTCATGAACTCAAGCATGGCAATCACAAATATGATTGGGCCACTGGAACAAATGGCTTTAGCTGATCACCCCATTAAAGGAATCTACTTTATGGTGGTCGGTTCACCTCAGGAACAAAGACTAATAGTATGTTCCATGATAGTTTTACATTATACAAGCACTAACCGGAAGAAAAGCAACAACAACATAATGATTATTTTGGGAAGAAAAAAAAATCAAACTGAAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1298 WES_acyltransf

Protein Analysis

447

Amino Acids

50.54

Weight (kDa)

9.04

Isoelectric Point (pI)

41.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WS_DGAT_C PF06974 372 - 410 1.2e-08 WS/DGAT C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000634)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10290 FvH4_3g28230 FvH4_3g28240
malus_domestica MD05G1036400.v1.1 MD10G1159400.v1.1 MD17G1237100.v1.1 MD17G1237200.v1.1 MD17G1237300.v1.1 MD17G1237600.v1.1 MD17G1238200.v1.1
prunus_persica Prupe.3G140400_v2.0.a1 Prupe.3G140700_v2.0.a1 Prupe.3G141100_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G258100_v2.0.a1 Prupe.7G117300_v2.0.a1 Prupe.7G117400_v2.0.a1 Prupe.7G117600_v2.0.a1 Prupe.8G044700_v2.0.a1
pyrus_communis pycom05g02680 pycom17g24220 pycom17g24240
rosa_chinensis RchiOBHm_Chr1g0350141 RchiOBHm_Chr5g0050931 RchiOBHm_Chr5g0051001 RchiOBHm_Chr5g0051021 RchiOBHm_Chr6g0267491
rosa_laevigata RLG00000013999 RLG00000028502 RLG00000034744 RLG00000034748 RLG00000034753
rosa_multiflora Rmu_sc0000446.1_g000056 Rmu_sc0000782.1_g000006 Rmu_sc0002951.1_g000001 Rmu_sc0003674.1_g000026 Rmu_sc0003756.1_g000004 Rmu_sc0011917.1_g000004 Rmu_sc0012005.1_g000019 Rmu_sc0040739.1_g000001
rosa_roxburghii Rroxscaffold_1G00029730 Rroxscaffold_1G00029740 Rroxscaffold_4G00305060 Rroxscaffold_7G00200710
rosa_rugosa Rorug01G0208400 Rorug05G0265200.1 Rorug05G0265300.1 Rorug05G0265400.1 Rorug05G0265500.1 Rorug05G0294000 Rorug06G0033100 Rorug06G0243300
rosa_samantha Rh1BG192100 Rh1BG192200 Rh1DG220600 Rh5AG270300 Rh5AG337200 Rh5AG337700 Rh5BG346700 Rh5BG347400 Rh5CG374300 Rh5DG359700 Rh5DG360500 Rh6AG154500 Rh6BG155100 Rh6BG362300 Rh6CG150300 Rh6DG140300 Rh6DG356300
rosa_wichuraiana Rw0G018820 Rw1G019350 Rw5G025300 Rw5G031770 Rw5G031820 Rw5G031870 Rw5G031910 Rw6G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 187
AccB1I GGYRCC 2 cut(s) 848, 1021
AccI GTMKAC 1 cut(s) 755
AciI CCGC 4 cut(s) 209, 397, 830, 938
AclI AACGTT 1 cut(s) 645
AclWI GGATC 1 cut(s) 460
AcuI CTGAAG 2 cut(s) 380, 1086
AfaI GTAC 2 cut(s) 428, 860
AfiI CCNNNNNNNGG 2 cut(s) 406, 1190
AgsI TTSAA 9 cut(s) 61, 203, 242, 299, 424, 463, 676, 692, 776
AhlI ACTAGT 1 cut(s) 587
AjnI CCWGG 3 cut(s) 43, 389, 401
AjuI GAANNNNNNNTTGG 4 cut(s) 106, 138, 967, 999
AluBI AGCT 6 cut(s) 250, 446, 802, 1098, 1106, 1175
AluI AGCT 6 cut(s) 250, 446, 802, 1098, 1106, 1175
Alw26I GTCTC 4 cut(s) 524, 603, 1021, 1081
AlwI GGATC 1 cut(s) 460
AoxI GGCC 3 cut(s) 475, 740, 1151
ApeKI GCWGC 2 cut(s) 443, 935
Asp700I GAANNNNTTC 1 cut(s) 1099
AspLEI GCGC 1 cut(s) 506
AspS9I GGNCC 2 cut(s) 714, 1151
AsuHPI GGTGA 4 cut(s) 49, 820, 1172, 1209
AvaII GGWCC 1 cut(s) 714
AxyI CCTNAGG 1 cut(s) 1221
BaeGI GKGCMC 3 cut(s) 683, 851, 1026
BanI GGYRCC 2 cut(s) 848, 1021
BanII GRGCYC 1 cut(s) 44
BarI GAAGNNNNNNTAC 3 cut(s) 9, 38, 41
BauI CACGAG 2 cut(s) 600, 911
BbsI GAAGAC 2 cut(s) 261, 975
BbvI GCAGC 2 cut(s) 455, 922
BccI CCATC 3 cut(s) 192, 479, 961
BceAI ACGGC 2 cut(s) 620, 921
BcgI CGANNNNNNTGC 4 cut(s) 745, 779, 892, 926
BciT130I CCWGG 3 cut(s) 45, 391, 403
BclI TGATCA 1 cut(s) 1177
BcoDI GTCTC 4 cut(s) 524, 603, 1021, 1081
BcuI ACTAGT 1 cut(s) 587
BfaI CTAG 3 cut(s) 285, 588, 927
BfoI RGCGCY 1 cut(s) 507
BfuAI ACCTGC 1 cut(s) 187
BisI GCNGC 2 cut(s) 444, 936
BlsI GCNGC 2 cut(s) 445, 937
Bme1390I CCNGG 3 cut(s) 45, 391, 403
Bme18I GGWCC 1 cut(s) 714
BmgT120I GGNCC 2 cut(s) 714, 1151
BmiI GGNNCC 5 cut(s) 33, 395, 850, 972, 1023
BmrFI CCNGG 3 cut(s) 45, 391, 403
BmsI GCATC 2 cut(s) 354, 753
BpiI GAAGAC 2 cut(s) 261, 975
BpmI CTGGAG 2 cut(s) 30, 50
BpuEI CTTGAG 1 cut(s) 1108
BsaJI CCNNGG 5 cut(s) 43, 375, 401, 472, 478
BsaWI WCCGGW 1 cut(s) 1278
Bsc4I CCNNNNNNNGG 2 cut(s) 406, 1190
Bse118I RCCGGY 1 cut(s) 34
Bse1I ACTGG 2 cut(s) 33, 1161
Bse21I CCTNAGG 1 cut(s) 1221
BseBI CCWGG 3 cut(s) 45, 391, 403
BseDI CCNNGG 5 cut(s) 43, 375, 401, 472, 478
BseGI GGATG 3 cut(s) 217, 385, 876
BseLI CCNNNNNNNGG 2 cut(s) 406, 1190
BseMII CTCAG 1 cut(s) 1235
BseNI ACTGG 2 cut(s) 33, 1161
BseSI GKGCMC 3 cut(s) 683, 851, 1026
BseXI GCAGC 2 cut(s) 455, 922
BsgI GTGCAG 1 cut(s) 974
BshFI GGCC 3 cut(s) 477, 742, 1153
BshNI GGYRCC 2 cut(s) 848, 1021
BsiSI CCGG 3 cut(s) 35, 717, 1279
BslFI GGGAC 1 cut(s) 254
BslI CCNNNNNNNGG 2 cut(s) 406, 1190
BsmAI GTCTC 4 cut(s) 524, 603, 1021, 1081
BsmFI GGGAC 1 cut(s) 254
BsnI GGCC 3 cut(s) 477, 742, 1153
Bsp1286I GDGCHC 4 cut(s) 44, 683, 851, 1026
Bsp1407I TGTACA 1 cut(s) 858
Bsp143I GATC 4 cut(s) 162, 465, 697, 1177
Bsp19I CCATGG 1 cut(s) 472
BspACI CCGC 4 cut(s) 209, 397, 830, 938
BspANI GGCC 3 cut(s) 477, 742, 1153
BspCNI CTCAG 1 cut(s) 1234
BspHI TCATGA 1 cut(s) 1116
BspLI GGNNCC 5 cut(s) 33, 395, 850, 972, 1023
BspMI ACCTGC 1 cut(s) 187
BspPI GGATC 1 cut(s) 460
BspQI GCTCTTC 1 cut(s) 1105
BspT107I GGYRCC 2 cut(s) 848, 1021
BsrFI RCCGGY 1 cut(s) 34
BsrGI TGTACA 1 cut(s) 858
BsrI ACTGG 2 cut(s) 33, 1161
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 5 cut(s) 43, 375, 401, 472, 478
BssMI GATC 4 cut(s) 162, 465, 697, 1177
BssSI CACGAG 2 cut(s) 600, 911
BssT1I CCWWGG 2 cut(s) 472, 478
Bst2BI CACGAG 2 cut(s) 600, 911
Bst2UI CCWGG 3 cut(s) 45, 391, 403
Bst4CI ACNGT 1 cut(s) 70
Bst6I CTCTTC 3 cut(s) 227, 549, 1105
BstAUI TGTACA 1 cut(s) 858
BstC8I GCNNGC 1 cut(s) 1108
BstDEI CTNAG 2 cut(s) 621, 1221
BstDSI CCRYGG 2 cut(s) 375, 472
BstF5I GGATG 3 cut(s) 217, 385, 876
BstH2I RGCGCY 1 cut(s) 507
BstHHI GCGC 1 cut(s) 506
BstKTI GATC 4 cut(s) 165, 468, 700, 1180
BstMAI GTCTC 4 cut(s) 524, 603, 1021, 1081
BstMBI GATC 4 cut(s) 162, 465, 697, 1177
BstMWI GCNNNNNNNGC 1 cut(s) 900
BstNI CCWGG 3 cut(s) 45, 391, 403
BstNSI RCATGY 1 cut(s) 1016
BstSCI CCNGG 3 cut(s) 43, 389, 401
BstSLI GKGCMC 3 cut(s) 683, 851, 1026
BstV1I GCAGC 2 cut(s) 455, 922
BstV2I GAAGAC 2 cut(s) 261, 975
BstX2I RGATCY 1 cut(s) 465
BstXI CCANNNNNNTGG 2 cut(s) 479, 1051
BstYI RGATCY 1 cut(s) 465
Bsu36I CCTNAGG 1 cut(s) 1221
BsuRI GGCC 3 cut(s) 477, 742, 1153
BtgI CCRYGG 2 cut(s) 375, 472
BtgZI GCGATG 1 cut(s) 498
BtsCI GGATG 3 cut(s) 217, 385, 876
BtsI GCAGTG 1 cut(s) 233
BtsIMutI CAGTG 3 cut(s) 66, 233, 1154
BveI ACCTGC 1 cut(s) 187
Cac8I GCNNGC 1 cut(s) 1108
CciI TCATGA 1 cut(s) 1116
CfoI GCGC 1 cut(s) 506
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 2 cut(s) 714, 1151
Csp6I GTAC 2 cut(s) 427, 859
CviQI GTAC 2 cut(s) 427, 859
DdeI CTNAG 2 cut(s) 621, 1221
DpnI GATC 4 cut(s) 164, 467, 699, 1179
DpnII GATC 4 cut(s) 162, 465, 697, 1177
Eam1104I CTCTTC 3 cut(s) 227, 549, 1105
EarI CTCTTC 3 cut(s) 227, 549, 1105
EciI GGCGGA 1 cut(s) 386
Eco130I CCWWGG 2 cut(s) 472, 478
Eco24I GRGCYC 1 cut(s) 44
Eco47I GGWCC 1 cut(s) 714
Eco57I CTGAAG 2 cut(s) 380, 1086
Eco81I CCTNAGG 1 cut(s) 1221
EcoRII CCWGG 3 cut(s) 43, 389, 401
EcoT14I CCWWGG 2 cut(s) 472, 478
EcoT38I GRGCYC 1 cut(s) 44
ErhI CCWWGG 2 cut(s) 472, 478
FalI AAGNNNNNCTT 2 cut(s) 967, 999
FaqI GGGAC 1 cut(s) 254
FauI CCCGC 2 cut(s) 823, 931
FbaI TGATCA 1 cut(s) 1177
FblI GTMKAC 1 cut(s) 755
Fnu4HI GCNGC 2 cut(s) 444, 936
FokI GGATG 3 cut(s) 224, 392, 883
FriOI GRGCYC 1 cut(s) 44
Fsp4HI GCNGC 2 cut(s) 444, 936
FspBI CTAG 3 cut(s) 285, 588, 927
GlaI GCGC 1 cut(s) 505
GluI GCNGC 2 cut(s) 444, 936
GsuI CTGGAG 2 cut(s) 30, 50
HaeII RGCGCY 1 cut(s) 507
HaeIII GGCC 3 cut(s) 477, 742, 1153
HapII CCGG 3 cut(s) 35, 717, 1279
HhaI GCGC 1 cut(s) 506
Hin6I GCGC 1 cut(s) 504
HinP1I GCGC 1 cut(s) 504
HincII GTYRAC 1 cut(s) 756
HindII GTYRAC 1 cut(s) 756
HindIII AAGCTT 1 cut(s) 800
HinfI GANTC 5 cut(s) 5, 101, 122, 299, 1194
HpaII CCGG 3 cut(s) 35, 717, 1279
HphI GGTGA 4 cut(s) 49, 820, 1172, 1209
Hpy166II GTNNAC 2 cut(s) 756, 1217
Hpy188I TCNGA 2 cut(s) 350, 369
Hpy188III TCNNGA 7 cut(s) 9, 160, 285, 463, 1084, 1117, 1223
Hpy8I GTNNAC 2 cut(s) 756, 1217
HpyAV CCTTC 6 cut(s) 11, 197, 355, 549, 570, 670
HpyCH4III ACNGT 1 cut(s) 70
HpyCH4IV ACGT 1 cut(s) 645
HpyCH4V TGCA 8 cut(s) 83, 443, 575, 650, 903, 955, 1012, 1110
HpyF10VI GCNNNNNNNGC 1 cut(s) 900
HpyF3I CTNAG 2 cut(s) 621, 1221
HpySE526I ACGT 1 cut(s) 645
HspAI GCGC 1 cut(s) 504
Ksp22I TGATCA 1 cut(s) 1177
Kzo9I GATC 4 cut(s) 162, 465, 697, 1177
LguI GCTCTTC 1 cut(s) 1105
LmnI GCTCC 2 cut(s) 31, 666
Lsp1109I GCAGC 2 cut(s) 455, 922
LweI GCATC 2 cut(s) 354, 753
MaeI CTAG 3 cut(s) 285, 588, 927
MaeII ACGT 1 cut(s) 645
MaeIII GTNAC 3 cut(s) 190, 808, 941
MalI GATC 4 cut(s) 164, 467, 699, 1179
MboI GATC 4 cut(s) 162, 465, 697, 1177
MflI RGATCY 1 cut(s) 465
MhlI GDGCHC 4 cut(s) 44, 683, 851, 1026
MluCI AATT 5 cut(s) 53, 84, 786, 898, 1339
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 2 cut(s) 644, 706
MnlI CCTC 4 cut(s) 167, 228, 292, 1230
MroXI GAANNNNTTC 1 cut(s) 1099
MseI TTAA 3 cut(s) 792, 1188, 1342
MslI CAYNNNNRTG 5 cut(s) 147, 264, 854, 1115, 1142
MspA1I CMGCKG 1 cut(s) 446
MspI CCGG 3 cut(s) 35, 717, 1279
MspR9I CCNGG 3 cut(s) 45, 391, 403
MvaI CCWGG 3 cut(s) 45, 391, 403
MwoI GCNNNNNNNGC 1 cut(s) 900
NcoI CCATGG 1 cut(s) 472
NdeII GATC 4 cut(s) 162, 465, 697, 1177
NlaIV GGNNCC 5 cut(s) 33, 395, 850, 972, 1023
NmuCI GTSAC 1 cut(s) 808
NspI RCATGY 1 cut(s) 1016
PagI TCATGA 1 cut(s) 1116
PciSI GCTCTTC 1 cut(s) 1105
PdmI GAANNNNTTC 1 cut(s) 1099
PfeI GAWTC 4 cut(s) 101, 122, 299, 1194
PkrI GCNGC 2 cut(s) 445, 937
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
Psp1406I AACGTT 1 cut(s) 645
Psp6I CCWGG 3 cut(s) 43, 389, 401
PspGI CCWGG 3 cut(s) 43, 389, 401
PspN4I GGNNCC 5 cut(s) 33, 395, 850, 972, 1023
PspPI GGNCC 2 cut(s) 714, 1151
PsuI RGATCY 1 cut(s) 465
PvuII CAGCTG 1 cut(s) 446
RsaI GTAC 2 cut(s) 428, 860
RsaNI GTAC 2 cut(s) 427, 859
RseI CAYNNNNRTG 5 cut(s) 147, 264, 854, 1115, 1142
SalI GTCGAC 1 cut(s) 754
SapI GCTCTTC 1 cut(s) 1105
SaqAI TTAA 3 cut(s) 792, 1188, 1342
SatI GCNGC 2 cut(s) 444, 936
Sau3AI GATC 4 cut(s) 162, 465, 697, 1177
Sau96I GGNCC 2 cut(s) 714, 1151
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 3 cut(s) 45, 391, 403
SduI GDGCHC 4 cut(s) 44, 683, 851, 1026
SfaNI GCATC 2 cut(s) 354, 753
SinI GGWCC 1 cut(s) 714
SmiMI CAYNNNNRTG 5 cut(s) 147, 264, 854, 1115, 1142
SmlI CTYRAG 1 cut(s) 1123
SmoI CTYRAG 1 cut(s) 1123
SpeI ACTAGT 1 cut(s) 587
Sse9I AATT 5 cut(s) 53, 84, 786, 898, 1339
SsiI CCGC 4 cut(s) 209, 397, 830, 938
SspI AATATT 1 cut(s) 459
SspMI CTAG 3 cut(s) 285, 588, 927
StyD4I CCNGG 3 cut(s) 43, 389, 401
StyI CCWWGG 2 cut(s) 472, 478
TaaI ACNGT 1 cut(s) 70
TaiI ACGT 1 cut(s) 648
TaqI TCGA 3 cut(s) 700, 755, 1031
TasI AATT 5 cut(s) 53, 84, 786, 898, 1339
TatI WGTACW 1 cut(s) 858
TfiI GAWTC 4 cut(s) 101, 122, 299, 1194
Tru1I TTAA 3 cut(s) 792, 1188, 1342
Tru9I TTAA 3 cut(s) 792, 1188, 1342
TscAI CASTG 3 cut(s) 73, 233, 1161
TseFI GTSAC 1 cut(s) 808
TseI GCWGC 2 cut(s) 443, 935
Tsp45I GTSAC 1 cut(s) 808
TspDTI ATGAA 5 cut(s) 228, 404, 597, 887, 1133
TspGWI ACGGA 3 cut(s) 82, 392, 883
TspRI CASTG 3 cut(s) 73, 233, 1161
VpaK11BI GGWCC 1 cut(s) 714
XbaI TCTAGA 1 cut(s) 284
XceI RCATGY 1 cut(s) 1016
XcmI CCANNNNNNNNNTGG 1 cut(s) 476
XmiI GTMKAC 1 cut(s) 755
XmnI GAANNNNTTC 1 cut(s) 1099
XspI CTAG 3 cut(s) 285, 588, 927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.