Rh5BG346700

O-acyltransferase (WSD1-like)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000634
Physical Location & Seq
Reverse (-)
52965776 .. 52989661
23886 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG346700.1

Sequence Viewer

Length: 1479 bp
ATGGAGTTTGGCGAAGAAGGATTACTACAAGAGCCGGTGAGCCCTGGTGGTCAGTTCATCAACAGCTCTGTCTTATCTCTTGCAATTCTTGCCGTTTTGGAATCACAAGTTCCAATCGACGATTCTCAAACATTTTTTCTTCTGAAAAATGTCTTCCTACCGATCAATCCACGCTTCTCTTCCATCATGGTTGAAGGTGCAGATAAAAAAAATAAGCAGTGGAAGAAGGTTACAGTGAAGCTGGAAGACCACGTCCATGTCCCCATTTTCCCCTCTGGAATGTCATCACCTGCATCATACGATATTTTCTTTGATGAGTATATAACGAAGATAGCATCAGAAACATTCCCACAAAGCAGGCCATTATGGGAACTTCATCTTTTCAAGTACCCAACAAGTCATGCAGCTGGACACATGATATTCAAGATCCACCATGCCCTTGGCGATGGCTACTCTCTCATGGGCGCTCTTCTCTCTTGCCTCCAGAATGCTCACAATCCTTCCATTCCTCTGACATTTCCGACATTAAAGAGTGCAAAAAATGAAACTAGTAGTCGATCTCGTGCGTTTGAGTTTATGCCGAAGATACTTTCTTCCGTTTTCAACGGTGCATGGGATTTCAGTTGGAGCATTTTGAAGGGCACTTGGGTTGAAGATGATCGAACACCAATAAGGTCCGGTGTTGAAGGAGTTGAGTTTCGACCTGTGTCGGTGTCAACCTTGATGCTGCCTATTGAGGAAATTAAACTTATCAAGAACAAGCTTGGAGTGACGATAAATGATGTGATTACAGGAACAATCTTTATGGGCATCCGAATGTACATTCAAGAGATGAATAGTGAAAAATCAAGTAGGCAAAATTGCACAGCACTGGTGTTGCTGAATACTAGACTAGTTGCGGGTTACAAGTCAGTGCAGGAGATGTTGATCGAAACAAATAAGTCTTGGGGAAACCGATTTGTGTTCTTGCAAGTCTCGGTGCCCAAGTCGAGTGAAGTTTCAAAGCCACTGGATTTTGTGTGGGAAGCACGTAATATAATCCAGAGACAGAGAAAATCTTCAGCTTGGTATCTCACCACTAGGCTCTGGGACATTTTGAAGAAATTTAGAGGCGCTGAGGCAGTATCTAAATATATCTATCGTACACTGAAAAACACAAGCATAGTAATCACAAATATGATTGGGCCATTGGAACAAATGAGTCTCGCCAATCACCCCATTGAAGGAATTTACTTCACAGTGACTGGTGCACCTCAGAGAAAATTGAAAAGAGAAATAAACATGTTTAAGGTTGAACGAGATGTATGTGTGCATCCTAACCCATCTCAAGCTAAACCCACCCGAAGAGTAAGAATATTAGCTAGAGCAACCCATGTCCAGCTGTTGACCACCAAGCCATCGACCATGACAATCCCGCCATATATTCATGGTATAAGCTTTGACAACTACAAATATGTCAGCCATTGCCTCAAGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1298 WES_acyltransf

Protein Analysis

492

Amino Acids

55.82

Weight (kDa)

9.48

Isoelectric Point (pI)

43.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WS_DGAT_cat PF03007 66 - 263 7.7e-12 Wax ester synthase/diacylglycerol acyltransferase catalytic domain
WS_DGAT_C PF06974 316 - 420 1.5e-24 WS/DGAT C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000634)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10290 FvH4_3g28230 FvH4_3g28240
malus_domestica MD05G1036400.v1.1 MD10G1159400.v1.1 MD17G1237100.v1.1 MD17G1237200.v1.1 MD17G1237300.v1.1 MD17G1237600.v1.1 MD17G1238200.v1.1
prunus_persica Prupe.3G140400_v2.0.a1 Prupe.3G140700_v2.0.a1 Prupe.3G141100_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G258100_v2.0.a1 Prupe.7G117300_v2.0.a1 Prupe.7G117400_v2.0.a1 Prupe.7G117600_v2.0.a1 Prupe.8G044700_v2.0.a1
pyrus_communis pycom05g02680 pycom17g24220 pycom17g24240
rosa_chinensis RchiOBHm_Chr1g0350141 RchiOBHm_Chr5g0050931 RchiOBHm_Chr5g0051001 RchiOBHm_Chr5g0051021 RchiOBHm_Chr6g0267491
rosa_laevigata RLG00000013999 RLG00000028502 RLG00000034744 RLG00000034748 RLG00000034753
rosa_multiflora Rmu_sc0000446.1_g000056 Rmu_sc0000782.1_g000006 Rmu_sc0002951.1_g000001 Rmu_sc0003674.1_g000026 Rmu_sc0003756.1_g000004 Rmu_sc0011917.1_g000004 Rmu_sc0012005.1_g000019 Rmu_sc0040739.1_g000001
rosa_roxburghii Rroxscaffold_1G00029730 Rroxscaffold_1G00029740 Rroxscaffold_4G00305060 Rroxscaffold_7G00200710
rosa_rugosa Rorug01G0208400 Rorug05G0265200.1 Rorug05G0265300.1 Rorug05G0265400.1 Rorug05G0265500.1 Rorug05G0294000 Rorug06G0033100 Rorug06G0243300
rosa_samantha Rh1BG192100 Rh1BG192200 Rh1DG220600 Rh5AG270300 Rh5AG337200 Rh5AG337700 Rh5BG346700 Rh5BG347400 Rh5CG374300 Rh5DG359700 Rh5DG360500 Rh6AG154500 Rh6BG155100 Rh6BG362300 Rh6CG150300 Rh6DG140300 Rh6DG356300
rosa_wichuraiana Rw0G018820 Rw1G019350 Rw5G025300 Rw5G031770 Rw5G031820 Rw5G031870 Rw5G031910 Rw6G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 298
Acc36I ACCTGC 1 cut(s) 298
AccB1I GGYRCC 1 cut(s) 979
AciI CCGC 2 cut(s) 899, 1415
AclWI GGATC 1 cut(s) 421
AcsI RAATTY 2 cut(s) 1103, 1227
AcuI CTGAAG 1 cut(s) 1044
AfaI GTAC 3 cut(s) 389, 821, 1144
AfiI CCNNNNNNNGG 1 cut(s) 1223
AflIII ACRYGT 1 cut(s) 1281
AhlI ACTAGT 2 cut(s) 548, 892
AjiI CACGTC 1 cut(s) 253
AjnI CCWGG 1 cut(s) 43
AjuI GAANNNNNNNTTGG 2 cut(s) 106, 138
AluBI AGCT 9 cut(s) 66, 241, 407, 763, 1064, 1331, 1361, 1381, 1437
AluI AGCT 9 cut(s) 66, 241, 407, 763, 1064, 1331, 1361, 1381, 1437
Alw21I GWGCWC 1 cut(s) 1252
Alw26I GTCTC 3 cut(s) 979, 1039, 1208
Alw44I GTGCAC 1 cut(s) 1248
AlwI GGATC 1 cut(s) 421
AlwNI CAGNNNCTG 1 cut(s) 1244
AoxI GGCC 2 cut(s) 359, 1184
ApaLI GTGCAC 1 cut(s) 1248
ApeKI GCWGC 2 cut(s) 404, 727
ApoI RAATTY 2 cut(s) 1103, 1227
Asp700I GAANNNNTTC 1 cut(s) 1057
AspLEI GCGC 2 cut(s) 467, 1115
AspS9I GGNCC 2 cut(s) 675, 1184
AsuHPI GGTGA 4 cut(s) 49, 279, 1066, 1205
AvaII GGWCC 1 cut(s) 675
BaeGI GKGCMC 3 cut(s) 644, 984, 1252
BanI GGYRCC 1 cut(s) 979
BanII GRGCYC 1 cut(s) 44
BarI GAAGNNNNNNTAC 3 cut(s) 9, 38, 41
BauI CACGAG 1 cut(s) 561
BbsI GAAGAC 2 cut(s) 145, 252
Bbv12I GWGCWC 1 cut(s) 1252
BbvCI CCTCAGC 1 cut(s) 1116
BbvI GCAGC 2 cut(s) 416, 714
BccI CCATC 4 cut(s) 191, 440, 1330, 1405
BceAI ACGGC 1 cut(s) 77
BciT130I CCWGG 1 cut(s) 45
BcoDI GTCTC 3 cut(s) 979, 1039, 1208
BcuI ACTAGT 2 cut(s) 548, 892
BfaI CTAG 5 cut(s) 549, 888, 893, 1080, 1362
BfoI RGCGCY 2 cut(s) 468, 1116
BfuAI ACCTGC 1 cut(s) 298
BisI GCNGC 2 cut(s) 405, 728
BlsI GCNGC 2 cut(s) 406, 729
Bme1390I CCNGG 1 cut(s) 45
Bme18I GGWCC 1 cut(s) 675
BmgBI CACGTC 1 cut(s) 253
BmgT120I GGNCC 2 cut(s) 675, 1184
BmiI GGNNCC 1 cut(s) 981
BmrFI CCNGG 1 cut(s) 45
BmsI GCATC 5 cut(s) 302, 344, 714, 819, 1321
BoxI GACNNNNGTC 1 cut(s) 706
BpiI GAAGAC 2 cut(s) 145, 252
BpmI CTGGAG 1 cut(s) 467
Bpu10I CCTNAGC 1 cut(s) 1116
BpuEI CTTGAG 2 cut(s) 1311, 1454
BsaAI YACGTR 1 cut(s) 1031
BsaBI GATNNNNATC 1 cut(s) 926
BsaJI CCNNGG 2 cut(s) 43, 439
BsaWI WCCGGW 1 cut(s) 677
Bsc4I CCNNNNNNNGG 1 cut(s) 1223
Bse118I RCCGGY 1 cut(s) 34
Bse1I ACTGG 3 cut(s) 876, 1014, 1249
Bse3DI GCAATG 1 cut(s) 1462
Bse8I GATNNNNATC 1 cut(s) 926
BseBI CCWGG 1 cut(s) 45
BseDI CCNNGG 2 cut(s) 43, 439
BseGI GGATG 2 cut(s) 810, 1312
BseJI GATNNNNATC 1 cut(s) 926
BseLI CCNNNNNNNGG 1 cut(s) 1223
BseMI GCAATG 1 cut(s) 1462
BseMII CTCAG 2 cut(s) 1107, 1268
BseNI ACTGG 3 cut(s) 876, 1014, 1249
BseSI GKGCMC 3 cut(s) 644, 984, 1252
BseXI GCAGC 2 cut(s) 416, 714
BsgI GTGCAG 2 cut(s) 219, 935
BshFI GGCC 2 cut(s) 361, 1186
BshNI GGYRCC 1 cut(s) 979
BsiHKAI GWGCWC 1 cut(s) 1252
BsiSI CCGG 2 cut(s) 35, 678
BslFI GGGAC 2 cut(s) 245, 1103
BslI CCNNNNNNNGG 1 cut(s) 1223
BsmAI GTCTC 3 cut(s) 979, 1039, 1208
BsmFI GGGAC 2 cut(s) 245, 1103
BsmI GAATGC 1 cut(s) 493
BsnI GGCC 2 cut(s) 361, 1186
Bsp1286I GDGCHC 4 cut(s) 44, 644, 984, 1252
Bsp1407I TGTACA 1 cut(s) 819
Bsp143I GATC 5 cut(s) 162, 426, 557, 658, 927
BspACI CCGC 2 cut(s) 899, 1415
BspANI GGCC 2 cut(s) 361, 1186
BspCNI CTCAG 2 cut(s) 1108, 1267
BspLI GGNNCC 1 cut(s) 981
BspMI ACCTGC 1 cut(s) 298
BspPI GGATC 1 cut(s) 421
BspQI GCTCTTC 1 cut(s) 474
BspT107I GGYRCC 1 cut(s) 979
BsrDI GCAATG 1 cut(s) 1462
BsrFI RCCGGY 1 cut(s) 34
BsrGI TGTACA 1 cut(s) 819
BsrI ACTGG 3 cut(s) 876, 1014, 1249
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 2 cut(s) 43, 439
BssMI GATC 5 cut(s) 162, 426, 557, 658, 927
BssSI CACGAG 1 cut(s) 561
BssT1I CCWWGG 1 cut(s) 439
Bst2BI CACGAG 1 cut(s) 561
Bst2UI CCWGG 1 cut(s) 45
Bst4CI ACNGT 3 cut(s) 235, 608, 1240
Bst6I CTCTTC 3 cut(s) 184, 474, 1339
BstAPI GCANNNNNTGC 1 cut(s) 89
BstAUI TGTACA 1 cut(s) 819
BstBAI YACGTR 1 cut(s) 1031
BstC8I GCNNGC 1 cut(s) 359
BstDEI CTNAG 2 cut(s) 1116, 1254
BstF5I GGATG 2 cut(s) 810, 1312
BstH2I RGCGCY 2 cut(s) 468, 1116
BstHHI GCGC 2 cut(s) 467, 1115
BstKTI GATC 5 cut(s) 165, 429, 560, 661, 930
BstMAI GTCTC 3 cut(s) 979, 1039, 1208
BstMBI GATC 5 cut(s) 162, 426, 557, 658, 927
BstMWI GCNNNNNNNGC 1 cut(s) 89
BstNI CCWGG 1 cut(s) 45
BstNSI RCATGY 1 cut(s) 1285
BstPAI GACNNNNGTC 1 cut(s) 706
BstSCI CCNGG 1 cut(s) 43
BstSLI GKGCMC 3 cut(s) 644, 984, 1252
BstV1I GCAGC 2 cut(s) 416, 714
BstV2I GAAGAC 2 cut(s) 145, 252
BstX2I RGATCY 1 cut(s) 426
BstXI CCANNNNNNTGG 1 cut(s) 440
BstYI RGATCY 1 cut(s) 426
BsuRI GGCC 2 cut(s) 361, 1186
BtgZI GCGATG 1 cut(s) 459
BtrI CACGTC 1 cut(s) 253
BtsCI GGATG 2 cut(s) 810, 1312
BtsI GCAGTG 1 cut(s) 224
BtsIMutI CAGTG 7 cut(s) 224, 240, 869, 918, 1007, 1145, 1245
BveI ACCTGC 1 cut(s) 298
Cac8I GCNNGC 1 cut(s) 359
CaiI CAGNNNCTG 1 cut(s) 1244
CfoI GCGC 2 cut(s) 467, 1115
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 2 cut(s) 675, 1184
Csp6I GTAC 3 cut(s) 388, 820, 1143
CviQI GTAC 3 cut(s) 388, 820, 1143
DdeI CTNAG 2 cut(s) 1116, 1254
DpnI GATC 5 cut(s) 164, 428, 559, 660, 929
DpnII GATC 5 cut(s) 162, 426, 557, 658, 927
Eam1104I CTCTTC 3 cut(s) 184, 474, 1339
EarI CTCTTC 3 cut(s) 184, 474, 1339
Eco130I CCWWGG 1 cut(s) 439
Eco24I GRGCYC 1 cut(s) 44
Eco47I GGWCC 1 cut(s) 675
Eco57I CTGAAG 1 cut(s) 1044
EcoRII CCWGG 1 cut(s) 43
EcoT14I CCWWGG 1 cut(s) 439
EcoT38I GRGCYC 1 cut(s) 44
ErhI CCWWGG 1 cut(s) 439
FaqI GGGAC 2 cut(s) 245, 1103
FauI CCCGC 2 cut(s) 892, 1422
Fnu4HI GCNGC 2 cut(s) 405, 728
FokI GGATG 2 cut(s) 797, 1299
FriOI GRGCYC 1 cut(s) 44
Fsp4HI GCNGC 2 cut(s) 405, 728
FspBI CTAG 5 cut(s) 549, 888, 893, 1080, 1362
GlaI GCGC 2 cut(s) 466, 1114
GluI GCNGC 2 cut(s) 405, 728
GsuI CTGGAG 1 cut(s) 467
HaeII RGCGCY 2 cut(s) 468, 1116
HaeIII GGCC 2 cut(s) 361, 1186
HapII CCGG 2 cut(s) 35, 678
HhaI GCGC 2 cut(s) 467, 1115
Hin6I GCGC 2 cut(s) 465, 1113
HinP1I GCGC 2 cut(s) 465, 1113
HincII GTYRAC 2 cut(s) 717, 1386
HindII GTYRAC 2 cut(s) 717, 1386
HindIII AAGCTT 2 cut(s) 761, 1435
HinfI GANTC 3 cut(s) 101, 122, 1201
HpaII CCGG 2 cut(s) 35, 678
HphI GGTGA 4 cut(s) 49, 279, 1066, 1205
Hpy166II GTNNAC 4 cut(s) 717, 1145, 1250, 1386
Hpy188I TCNGA 6 cut(s) 144, 340, 513, 522, 815, 1257
Hpy188III TCNNGA 7 cut(s) 276, 424, 484, 754, 827, 1042, 1471
Hpy8I GTNNAC 4 cut(s) 717, 1145, 1250, 1386
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 7 cut(s) 11, 188, 220, 510, 631, 680, 1217
HpyCH4III ACNGT 3 cut(s) 235, 608, 1240
HpyCH4IV ACGT 2 cut(s) 252, 1030
HpyF10VI GCNNNNNNNGC 1 cut(s) 89
HpyF3I CTNAG 2 cut(s) 1116, 1254
HpySE526I ACGT 2 cut(s) 252, 1030
HspAI GCGC 2 cut(s) 465, 1113
Kzo9I GATC 5 cut(s) 162, 426, 557, 658, 927
LguI GCTCTTC 1 cut(s) 474
LmnI GCTCC 1 cut(s) 627
Lsp1109I GCAGC 2 cut(s) 416, 714
LweI GCATC 5 cut(s) 302, 344, 714, 819, 1321
MaeI CTAG 5 cut(s) 549, 888, 893, 1080, 1362
MaeII ACGT 2 cut(s) 252, 1030
MaeIII GTNAC 4 cut(s) 229, 769, 902, 1240
MalI GATC 5 cut(s) 164, 428, 559, 660, 929
MboI GATC 5 cut(s) 162, 426, 557, 658, 927
MflI RGATCY 1 cut(s) 426
MhlI GDGCHC 4 cut(s) 44, 644, 984, 1252
MluCI AATT 6 cut(s) 84, 741, 859, 1103, 1227, 1262
MlyI GAGTC 1 cut(s) 1210
MmeI TCCRAC 2 cut(s) 545, 605
MnlI CCTC 8 cut(s) 283, 491, 519, 730, 1103, 1111, 1263, 1478
MroXI GAANNNNTTC 1 cut(s) 1057
MseI TTAA 3 cut(s) 527, 744, 1287
MslI CAYNNNNRTG 3 cut(s) 255, 815, 1175
MspA1I CMGCKG 2 cut(s) 407, 1381
MspI CCGG 2 cut(s) 35, 678
MspR9I CCNGG 1 cut(s) 45
Mva1269I GAATGC 1 cut(s) 493
MvaI CCWGG 1 cut(s) 45
MwoI GCNNNNNNNGC 1 cut(s) 89
NdeII GATC 5 cut(s) 162, 426, 557, 658, 927
NlaIV GGNNCC 1 cut(s) 981
NmuCI GTSAC 2 cut(s) 769, 1240
NspI RCATGY 1 cut(s) 1285
PaqCI CACCTGC 1 cut(s) 298
PciI ACATGT 1 cut(s) 1281
PciSI GCTCTTC 1 cut(s) 474
PctI GAATGC 1 cut(s) 493
PdmI GAANNNNTTC 1 cut(s) 1057
PfeI GAWTC 2 cut(s) 101, 122
PflFI GACNNNGTC 1 cut(s) 251
PkrI GCNGC 2 cut(s) 406, 729
PleI GAGTC 1 cut(s) 1209
PpsI GAGTC 1 cut(s) 1209
Ppu21I YACGTR 1 cut(s) 1031
PscI ACATGT 1 cut(s) 1281
PshAI GACNNNNGTC 1 cut(s) 706
Psp6I CCWGG 1 cut(s) 43
PspGI CCWGG 1 cut(s) 43
PspN4I GGNNCC 1 cut(s) 981
PspPI GGNCC 2 cut(s) 675, 1184
PstNI CAGNNNCTG 1 cut(s) 1244
PsuI RGATCY 1 cut(s) 426
PsyI GACNNNGTC 1 cut(s) 251
PvuII CAGCTG 2 cut(s) 407, 1381
RsaI GTAC 3 cut(s) 389, 821, 1144
RsaNI GTAC 3 cut(s) 388, 820, 1143
RseI CAYNNNNRTG 3 cut(s) 255, 815, 1175
SapI GCTCTTC 1 cut(s) 474
SaqAI TTAA 3 cut(s) 527, 744, 1287
SatI GCNGC 2 cut(s) 405, 728
Sau3AI GATC 5 cut(s) 162, 426, 557, 658, 927
Sau96I GGNCC 2 cut(s) 675, 1184
SchI GAGTC 1 cut(s) 1210
ScrFI CCNGG 1 cut(s) 45
SduI GDGCHC 4 cut(s) 44, 644, 984, 1252
SfaNI GCATC 5 cut(s) 302, 344, 714, 819, 1321
SinI GGWCC 1 cut(s) 675
SmiMI CAYNNNNRTG 3 cut(s) 255, 815, 1175
SmlI CTYRAG 2 cut(s) 1326, 1469
SmoI CTYRAG 2 cut(s) 1326, 1469
SpeI ACTAGT 2 cut(s) 548, 892
Sse9I AATT 6 cut(s) 84, 741, 859, 1103, 1227, 1262
SsiI CCGC 2 cut(s) 899, 1415
SspI AATATT 1 cut(s) 1356
SspMI CTAG 5 cut(s) 549, 888, 893, 1080, 1362
StyD4I CCNGG 1 cut(s) 43
StyI CCWWGG 1 cut(s) 439
TaaI ACNGT 3 cut(s) 235, 608, 1240
TaiI ACGT 2 cut(s) 255, 1033
TaqI TCGA 7 cut(s) 117, 556, 661, 700, 930, 989, 1400
TasI AATT 6 cut(s) 84, 741, 859, 1103, 1227, 1262
TatI WGTACW 1 cut(s) 819
TfiI GAWTC 2 cut(s) 101, 122
Tru1I TTAA 3 cut(s) 527, 744, 1287
Tru9I TTAA 3 cut(s) 527, 744, 1287
TscAI CASTG 7 cut(s) 224, 240, 876, 918, 1014, 1152, 1245
TseFI GTSAC 2 cut(s) 769, 1240
TseI GCWGC 2 cut(s) 404, 727
Tsp45I GTSAC 2 cut(s) 769, 1240
TspDTI ATGAA 5 cut(s) 46, 365, 558, 848, 1415
TspGWI ACGGA 1 cut(s) 586
TspRI CASTG 7 cut(s) 224, 240, 876, 918, 1014, 1152, 1245
Tth111I GACNNNGTC 1 cut(s) 251
VneI GTGCAC 1 cut(s) 1248
VpaK11BI GGWCC 1 cut(s) 675
XapI RAATTY 2 cut(s) 1103, 1227
XceI RCATGY 1 cut(s) 1285
XcmI CCANNNNNNNNNTGG 1 cut(s) 437
XmnI GAANNNNTTC 1 cut(s) 1057
XspI CTAG 5 cut(s) 549, 888, 893, 1080, 1362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.