RLG00000034748

O-acyltransferase (WSD1-like)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000634
Physical Location & Seq
Reverse (-)
53624922 .. 53629420
4499 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034748

Sequence Viewer

Length: 1407 bp
ATGGAGCTTGGCGAAGAGGGATTACTACAAGAGCCGGTGAGCCCTGGTGGTCAGTTCATCAACAGCTCTGTCTTATCTCTTGCAATTCTTGCCGTTTTGGAATCACAAGTTCCAATCGACGATTCTCAAACGTTTTTCCTTCTGAAAAATGTCTTCCTCCCTATCAATCCACGCTTCTCTTCCATCATGGTTGAAGGTGTAGATAAAAAGAATAAGCAGTGGAAGAAGGTTATAGTGAAGGTGGAAGACCACGTCCATGTCCCCATTTTCCCCTCTGGAATGTCACCTGCATCATACGATATTTACTTTGATGAGTATATAACGAAGATAGCATCAGAAACATTCCCACAAAGCAGACCATTATGGGAACTTCATCTTTTCAAGTACCCAACAAGTCATGCAGCTGGACAAATGATATTCAAGATCCACCATGCCCTTGGCGATGGCTACTCTCTCATGGGCGCTCTTCTCTCTTGCCTCCAAAATGCTCACAATCCTTCCATTCCCCTGACATTTCCTACATTAAAGAGTGCAAAAAATGAAACTAGTAGTCGATCTGGTGCGTTTGAGTTTATGCCGAAGATACTTTCTTCCATTTTCAACGGTGCATGGGATTTCAGTTGGAGCATTTTGAAGGGCACTTGGGTTGAAGATGATCGAACACCAATAAGGTCCGGTGTTGAAGGAGTTGAGTTTCGACCTGTGTCGGTGTCAACCTTGATGCTGCCTATTGAGGAAATTAAACTTATCAAGAACAAGCTTGGAGTGACAATAAATGATGTGATTACAGGAACAATCTTTATGGGCATCCGAATGTACATTCAAGAGATGAATAGTGAAAAATCAAGTAGCCAAAATTGCACAGCACTGGTGTTGCTGAATACTAGACTTGTTGCGGGTTACAAGTCAGTGCAGGAGATGTTGATCGAAACAAACAAGTCTTGGGGAAACCGATTTGTGTTCTTGCAAGTCTCGGTGCCCAAGTCGAGTGAAGTTTCAAAGCCACTGGATTTTGTGTGGGAAGCACATAATATAATCAAGAGACAGAGAAAATCTTCAGCTTGGTACCTCACCACTAGGCTCTGGGACATTTTGAAGAAATTTAGAGGCCCTGAGGATGAACTCGGTGACCATGACGGCGCCGAGCCGGATTCCGAAGAGCTCCTGCACTTGTCTCGCGTTTTTCATGGACTGTTTTGCGAGGACGGCGATTTTGATCCAGAAACGGTTGGCGAAGGTGAGGACCATGGAGTTGGGGCTGGCGTCATTGTTGGTGGCGCCGGAGACGAGCTTCCGCGCCGGATAGCTTTTCCAAACCAGATTCTGATTCCTCCGCGCTGTCTCTCTCCGCCGCGCCGTAGTCGAGGCTTTGGAGACGAGCTTCCTCTCCGTAGCCGAAGTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1298 WES_acyltransf

Protein Analysis

469

Amino Acids

52.37

Weight (kDa)

5.86

Isoelectric Point (pI)

53.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WS_DGAT_cat PF03007 103 - 262 6.4e-10 Wax ester synthase/diacylglycerol acyltransferase catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000634)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10290 FvH4_3g28230 FvH4_3g28240
malus_domestica MD05G1036400.v1.1 MD10G1159400.v1.1 MD17G1237100.v1.1 MD17G1237200.v1.1 MD17G1237300.v1.1 MD17G1237600.v1.1 MD17G1238200.v1.1
prunus_persica Prupe.3G140400_v2.0.a1 Prupe.3G140700_v2.0.a1 Prupe.3G141100_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G258100_v2.0.a1 Prupe.7G117300_v2.0.a1 Prupe.7G117400_v2.0.a1 Prupe.7G117600_v2.0.a1 Prupe.8G044700_v2.0.a1
pyrus_communis pycom05g02680 pycom17g24220 pycom17g24240
rosa_chinensis RchiOBHm_Chr1g0350141 RchiOBHm_Chr5g0050931 RchiOBHm_Chr5g0051001 RchiOBHm_Chr5g0051021 RchiOBHm_Chr6g0267491
rosa_laevigata RLG00000013999 RLG00000028502 RLG00000034744 RLG00000034748 RLG00000034753
rosa_multiflora Rmu_sc0000446.1_g000056 Rmu_sc0000782.1_g000006 Rmu_sc0002951.1_g000001 Rmu_sc0003674.1_g000026 Rmu_sc0003756.1_g000004 Rmu_sc0011917.1_g000004 Rmu_sc0012005.1_g000019 Rmu_sc0040739.1_g000001
rosa_roxburghii Rroxscaffold_1G00029730 Rroxscaffold_1G00029740 Rroxscaffold_4G00305060 Rroxscaffold_7G00200710
rosa_rugosa Rorug01G0208400 Rorug05G0265200.1 Rorug05G0265300.1 Rorug05G0265400.1 Rorug05G0265500.1 Rorug05G0294000 Rorug06G0033100 Rorug06G0243300
rosa_samantha Rh1BG192100 Rh1BG192200 Rh1DG220600 Rh5AG270300 Rh5AG337200 Rh5AG337700 Rh5BG346700 Rh5BG347400 Rh5CG374300 Rh5DG359700 Rh5DG360500 Rh6AG154500 Rh6BG155100 Rh6BG362300 Rh6CG150300 Rh6DG140300 Rh6DG356300
rosa_wichuraiana Rw0G018820 Rw1G019350 Rw5G025300 Rw5G031770 Rw5G031820 Rw5G031870 Rw5G031910 Rw6G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 295
Acc36I ACCTGC 1 cut(s) 295
Acc65I GGTACC 1 cut(s) 1065
AccB1I GGYRCC 4 cut(s) 976, 1065, 1139, 1277
AccII CGCG 4 cut(s) 1179, 1297, 1336, 1354
AciI CCGC 5 cut(s) 896, 1295, 1334, 1349, 1352
AclI AACGTT 1 cut(s) 131
AclWI GGATC 2 cut(s) 418, 1211
AcsI RAATTY 1 cut(s) 1100
AcuI CTGAAG 1 cut(s) 1041
AcyI GRCGYC 3 cut(s) 1140, 1263, 1278
AfaI GTAC 3 cut(s) 386, 818, 1067
AhlI ACTAGT 1 cut(s) 545
AjiI CACGTC 1 cut(s) 253
AjnI CCWGG 1 cut(s) 43
AjuI GAANNNNNNNTTGG 2 cut(s) 106, 138
AluBI AGCT 9 cut(s) 7, 66, 404, 760, 1061, 1162, 1291, 1307, 1381
AluI AGCT 9 cut(s) 7, 66, 404, 760, 1061, 1162, 1291, 1307, 1381
Alw21I GWGCWC 1 cut(s) 1164
Alw26I GTCTC 6 cut(s) 976, 1036, 1179, 1278, 1346, 1368
AlwI GGATC 2 cut(s) 418, 1211
AlwNI CAGNNNCTG 1 cut(s) 1324
AoxI GGCC 1 cut(s) 1108
ApeKI GCWGC 2 cut(s) 401, 724
ApoI RAATTY 1 cut(s) 1100
ArsI GACNNNNNNTTYG 2 cut(s) 1196, 1228
Asp700I GAANNNNTTC 1 cut(s) 1054
Asp718I GGTACC 1 cut(s) 1065
AspLEI GCGC 6 cut(s) 464, 1142, 1280, 1299, 1338, 1356
AspS9I GGNCC 3 cut(s) 672, 1109, 1243
AsuHPI GGTGA 5 cut(s) 49, 276, 1063, 1139, 1250
AvaII GGWCC 2 cut(s) 672, 1243
AxyI CCTNAGG 1 cut(s) 1113
BaeGI GKGCMC 2 cut(s) 641, 981
BanI GGYRCC 4 cut(s) 976, 1065, 1139, 1277
BanII GRGCYC 2 cut(s) 44, 1164
BarI GAAGNNNNNNTAC 1 cut(s) 38
BbsI GAAGAC 2 cut(s) 145, 252
Bbv12I GWGCWC 1 cut(s) 1164
BbvI GCAGC 2 cut(s) 413, 711
BccI CCATC 2 cut(s) 191, 437
BceAI ACGGC 4 cut(s) 77, 1153, 1222, 1341
BcgI CGANNNNNNTGC 2 cut(s) 1157, 1191
BciT130I CCWGG 1 cut(s) 45
BcoDI GTCTC 6 cut(s) 976, 1036, 1179, 1278, 1346, 1368
BcuI ACTAGT 1 cut(s) 545
BfaI CTAG 3 cut(s) 546, 885, 1077
BfoI RGCGCY 3 cut(s) 465, 1143, 1281
BfuAI ACCTGC 1 cut(s) 295
BisI GCNGC 3 cut(s) 402, 725, 1352
BlsI GCNGC 3 cut(s) 403, 726, 1353
Bme1390I CCNGG 1 cut(s) 45
Bme18I GGWCC 2 cut(s) 672, 1243
BmgBI CACGTC 1 cut(s) 253
BmgT120I GGNCC 3 cut(s) 672, 1109, 1243
BmiI GGNNCC 4 cut(s) 978, 1067, 1141, 1279
BmrFI CCNGG 1 cut(s) 45
BmsI GCATC 4 cut(s) 299, 341, 711, 816
BoxI GACNNNNGTC 1 cut(s) 703
BpiI GAAGAC 2 cut(s) 145, 252
BsaBI GATNNNNATC 2 cut(s) 923, 1215
BsaHI GRCGYC 3 cut(s) 1140, 1263, 1278
BsaJI CCNNGG 3 cut(s) 43, 436, 1246
BsaWI WCCGGW 1 cut(s) 674
Bse118I RCCGGY 1 cut(s) 34
Bse1I ACTGG 2 cut(s) 873, 1011
Bse21I CCTNAGG 1 cut(s) 1113
Bse8I GATNNNNATC 2 cut(s) 923, 1215
BseBI CCWGG 1 cut(s) 45
BseDI CCNNGG 3 cut(s) 43, 436, 1246
BseGI GGATG 2 cut(s) 807, 1123
BseJI GATNNNNATC 2 cut(s) 923, 1215
BseMII CTCAG 1 cut(s) 1104
BseNI ACTGG 2 cut(s) 873, 1011
BseSI GKGCMC 2 cut(s) 641, 981
BseXI GCAGC 2 cut(s) 413, 711
BsgI GTGCAG 2 cut(s) 932, 1151
Bsh1236I CGCG 4 cut(s) 1179, 1297, 1336, 1354
BshFI GGCC 1 cut(s) 1110
BshNI GGYRCC 4 cut(s) 976, 1065, 1139, 1277
BsiHKAI GWGCWC 1 cut(s) 1164
BsiSI CCGG 5 cut(s) 35, 675, 1148, 1281, 1300
BslFI GGGAC 2 cut(s) 245, 1100
BsmAI GTCTC 6 cut(s) 976, 1036, 1179, 1278, 1346, 1368
BsmBI CGTCTC 2 cut(s) 1278, 1368
BsmFI GGGAC 2 cut(s) 245, 1100
BsnI GGCC 1 cut(s) 1110
Bsp1286I GDGCHC 4 cut(s) 44, 641, 981, 1164
Bsp1407I TGTACA 1 cut(s) 816
Bsp143I GATC 5 cut(s) 423, 554, 655, 924, 1216
Bsp19I CCATGG 1 cut(s) 1246
BspACI CCGC 5 cut(s) 896, 1295, 1334, 1349, 1352
BspANI GGCC 1 cut(s) 1110
BspCNI CTCAG 1 cut(s) 1105
BspFNI CGCG 4 cut(s) 1179, 1297, 1336, 1354
BspLI GGNNCC 4 cut(s) 978, 1067, 1141, 1279
BspMI ACCTGC 1 cut(s) 295
BspPI GGATC 2 cut(s) 418, 1211
BspQI GCTCTTC 2 cut(s) 471, 1152
BspT107I GGYRCC 4 cut(s) 976, 1065, 1139, 1277
BsrFI RCCGGY 1 cut(s) 34
BsrGI TGTACA 1 cut(s) 816
BsrI ACTGG 2 cut(s) 873, 1011
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 3 cut(s) 43, 436, 1246
BssMI GATC 5 cut(s) 423, 554, 655, 924, 1216
BssNI GRCGYC 3 cut(s) 1140, 1263, 1278
BssT1I CCWWGG 2 cut(s) 436, 1246
Bst2UI CCWGG 1 cut(s) 45
Bst4CI ACNGT 3 cut(s) 605, 1194, 1228
Bst6I CTCTTC 4 cut(s) 9, 184, 471, 1152
BstACI GRCGYC 3 cut(s) 1140, 1263, 1278
BstAPI GCANNNNNTGC 1 cut(s) 89
BstAUI TGTACA 1 cut(s) 816
BstC8I GCNNGC 1 cut(s) 1261
BstDEI CTNAG 1 cut(s) 1113
BstDSI CCRYGG 1 cut(s) 1246
BstEII GGTNACC 1 cut(s) 1127
BstF5I GGATG 2 cut(s) 807, 1123
BstFNI CGCG 4 cut(s) 1179, 1297, 1336, 1354
BstH2I RGCGCY 3 cut(s) 465, 1143, 1281
BstHHI GCGC 6 cut(s) 464, 1142, 1280, 1299, 1338, 1356
BstKTI GATC 5 cut(s) 426, 557, 658, 927, 1219
BstMAI GTCTC 6 cut(s) 976, 1036, 1179, 1278, 1346, 1368
BstMBI GATC 5 cut(s) 423, 554, 655, 924, 1216
BstMWI GCNNNNNNNGC 3 cut(s) 89, 858, 1206
BstNI CCWGG 1 cut(s) 45
BstPAI GACNNNNGTC 1 cut(s) 703
BstPI GGTNACC 1 cut(s) 1127
BstSCI CCNGG 1 cut(s) 43
BstSLI GKGCMC 2 cut(s) 641, 981
BstUI CGCG 4 cut(s) 1179, 1297, 1336, 1354
BstV1I GCAGC 2 cut(s) 413, 711
BstV2I GAAGAC 2 cut(s) 145, 252
BstX2I RGATCY 1 cut(s) 423
BstXI CCANNNNNNTGG 2 cut(s) 437, 1253
BstYI RGATCY 1 cut(s) 423
Bsu36I CCTNAGG 1 cut(s) 1113
BsuRI GGCC 1 cut(s) 1110
BtgI CCRYGG 1 cut(s) 1246
BtgZI GCGATG 1 cut(s) 456
BtrI CACGTC 1 cut(s) 253
BtsCI GGATG 2 cut(s) 807, 1123
BtsI GCAGTG 1 cut(s) 224
BtsIMutI CAGTG 5 cut(s) 224, 866, 915, 1004, 1402
BveI ACCTGC 1 cut(s) 295
Cac8I GCNNGC 1 cut(s) 1261
CaiI CAGNNNCTG 1 cut(s) 1324
CfoI GCGC 6 cut(s) 464, 1142, 1280, 1299, 1338, 1356
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 3 cut(s) 672, 1109, 1243
CseI GACGC 1 cut(s) 1252
Csp6I GTAC 3 cut(s) 385, 817, 1066
CviAII CATG 9 cut(s) 187, 257, 398, 431, 457, 609, 1133, 1187, 1247
CviQI GTAC 3 cut(s) 385, 817, 1066
DdeI CTNAG 1 cut(s) 1113
DinI GGCGCC 2 cut(s) 1141, 1279
DpnI GATC 5 cut(s) 425, 556, 657, 926, 1218
DpnII GATC 5 cut(s) 423, 554, 655, 924, 1216
Eam1104I CTCTTC 4 cut(s) 9, 184, 471, 1152
EarI CTCTTC 4 cut(s) 9, 184, 471, 1152
EciI GGCGGA 1 cut(s) 1338
Ecl136II GAGCTC 1 cut(s) 1162
Eco130I CCWWGG 2 cut(s) 436, 1246
Eco24I GRGCYC 2 cut(s) 44, 1164
Eco47I GGWCC 2 cut(s) 672, 1243
Eco53kI GAGCTC 1 cut(s) 1162
Eco57I CTGAAG 1 cut(s) 1041
Eco81I CCTNAGG 1 cut(s) 1113
Eco91I GGTNACC 1 cut(s) 1127
EcoICRI GAGCTC 1 cut(s) 1162
EcoO109I RGGNCCY 1 cut(s) 1109
EcoO65I GGTNACC 1 cut(s) 1127
EcoRII CCWGG 1 cut(s) 43
EcoT14I CCWWGG 2 cut(s) 436, 1246
EcoT38I GRGCYC 2 cut(s) 44, 1164
EgeI GGCGCC 2 cut(s) 1141, 1279
EheI GGCGCC 2 cut(s) 1141, 1279
ErhI CCWWGG 2 cut(s) 436, 1246
Esp3I CGTCTC 2 cut(s) 1278, 1368
FaeI CATG 9 cut(s) 190, 260, 401, 434, 460, 612, 1136, 1190, 1250
FaqI GGGAC 2 cut(s) 245, 1100
FatI CATG 9 cut(s) 186, 256, 397, 430, 456, 608, 1132, 1186, 1246
FauI CCCGC 1 cut(s) 889
Fnu4HI GCNGC 3 cut(s) 402, 725, 1352
FokI GGATG 2 cut(s) 794, 1130
FriOI GRGCYC 2 cut(s) 44, 1164
Fsp4HI GCNGC 3 cut(s) 402, 725, 1352
FspBI CTAG 3 cut(s) 546, 885, 1077
GlaI GCGC 6 cut(s) 463, 1141, 1279, 1298, 1337, 1355
GluI GCNGC 3 cut(s) 402, 725, 1352
HaeII RGCGCY 3 cut(s) 465, 1143, 1281
HaeIII GGCC 1 cut(s) 1110
HapII CCGG 5 cut(s) 35, 675, 1148, 1281, 1300
HgaI GACGC 1 cut(s) 1252
HhaI GCGC 6 cut(s) 464, 1142, 1280, 1299, 1338, 1356
Hin1I GRCGYC 3 cut(s) 1140, 1263, 1278
Hin1II CATG 9 cut(s) 190, 260, 401, 434, 460, 612, 1136, 1190, 1250
Hin6I GCGC 6 cut(s) 462, 1140, 1278, 1297, 1336, 1354
HinP1I GCGC 6 cut(s) 462, 1140, 1278, 1297, 1336, 1354
HincII GTYRAC 1 cut(s) 714
HindII GTYRAC 1 cut(s) 714
HindIII AAGCTT 1 cut(s) 758
HinfI GANTC 5 cut(s) 101, 122, 1151, 1321, 1327
HpaII CCGG 5 cut(s) 35, 675, 1148, 1281, 1300
HphI GGTGA 5 cut(s) 49, 276, 1063, 1139, 1250
Hpy166II GTNNAC 1 cut(s) 714
Hpy188I TCNGA 5 cut(s) 144, 337, 812, 1156, 1326
Hpy188III TCNNGA 6 cut(s) 276, 421, 751, 824, 1039, 1220
Hpy8I GTNNAC 1 cut(s) 714
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 8 cut(s) 149, 188, 220, 232, 507, 628, 677, 1229
HpyCH4III ACNGT 3 cut(s) 605, 1194, 1228
HpyCH4IV ACGT 2 cut(s) 131, 252
HpyCH4V TGCA 9 cut(s) 83, 290, 401, 533, 608, 861, 913, 967, 1168
HpyF10VI GCNNNNNNNGC 3 cut(s) 89, 858, 1206
HpyF3I CTNAG 1 cut(s) 1113
HpySE526I ACGT 2 cut(s) 131, 252
Hsp92I GRCGYC 3 cut(s) 1140, 1263, 1278
Hsp92II CATG 9 cut(s) 190, 260, 401, 434, 460, 612, 1136, 1190, 1250
HspAI GCGC 6 cut(s) 462, 1140, 1278, 1297, 1336, 1354
KasI GGCGCC 2 cut(s) 1139, 1277
KpnI GGTACC 1 cut(s) 1069
Kzo9I GATC 5 cut(s) 423, 554, 655, 924, 1216
LguI GCTCTTC 2 cut(s) 471, 1152
LmnI GCTCC 3 cut(s) 4, 624, 1167
Lsp1109I GCAGC 2 cut(s) 413, 711
LweI GCATC 4 cut(s) 299, 341, 711, 816
MaeI CTAG 3 cut(s) 546, 885, 1077
MaeII ACGT 2 cut(s) 131, 252
MaeIII GTNAC 5 cut(s) 282, 766, 899, 1127, 1400
MalI GATC 5 cut(s) 425, 556, 657, 926, 1218
MboI GATC 5 cut(s) 423, 554, 655, 924, 1216
MflI RGATCY 1 cut(s) 423
MhlI GDGCHC 4 cut(s) 44, 641, 981, 1164
MluCI AATT 4 cut(s) 84, 738, 856, 1100
Mly113I GGCGCC 2 cut(s) 1140, 1278
MmeI TCCRAC 1 cut(s) 602
MroXI GAANNNNTTC 1 cut(s) 1054
MseI TTAA 2 cut(s) 524, 741
MslI CAYNNNNRTG 2 cut(s) 255, 812
MspA1I CMGCKG 1 cut(s) 404
MspI CCGG 5 cut(s) 35, 675, 1148, 1281, 1300
MspR9I CCNGG 1 cut(s) 45
MvaI CCWGG 1 cut(s) 45
MvnI CGCG 4 cut(s) 1179, 1297, 1336, 1354
MwoI GCNNNNNNNGC 3 cut(s) 89, 858, 1206
NarI GGCGCC 2 cut(s) 1140, 1278
NcoI CCATGG 1 cut(s) 1246
NdeII GATC 5 cut(s) 423, 554, 655, 924, 1216
NlaIII CATG 9 cut(s) 190, 260, 401, 434, 460, 612, 1136, 1190, 1250
NlaIV GGNNCC 4 cut(s) 978, 1067, 1141, 1279
NmeAIII GCCGAG 1 cut(s) 1168
NmuCI GTSAC 4 cut(s) 282, 766, 1127, 1400
PaqCI CACCTGC 1 cut(s) 295
PciSI GCTCTTC 2 cut(s) 471, 1152
PdmI GAANNNNTTC 1 cut(s) 1054
PfeI GAWTC 5 cut(s) 101, 122, 1151, 1321, 1327
PflFI GACNNNGTC 1 cut(s) 251
PkrI GCNGC 3 cut(s) 403, 726, 1353
PluTI GGCGCC 2 cut(s) 1143, 1281
PshAI GACNNNNGTC 1 cut(s) 703
Psp124BI GAGCTC 1 cut(s) 1164
Psp1406I AACGTT 1 cut(s) 131
Psp6I CCWGG 1 cut(s) 43
PspEI GGTNACC 1 cut(s) 1127
PspGI CCWGG 1 cut(s) 43
PspN4I GGNNCC 4 cut(s) 978, 1067, 1141, 1279
PspPI GGNCC 3 cut(s) 672, 1109, 1243
PstNI CAGNNNCTG 1 cut(s) 1324
PsuI RGATCY 1 cut(s) 423
PsyI GACNNNGTC 1 cut(s) 251
PvuII CAGCTG 1 cut(s) 404
RsaI GTAC 3 cut(s) 386, 818, 1067
RsaNI GTAC 3 cut(s) 385, 817, 1066
RseI CAYNNNNRTG 2 cut(s) 255, 812
SacI GAGCTC 1 cut(s) 1164
SapI GCTCTTC 2 cut(s) 471, 1152
SaqAI TTAA 2 cut(s) 524, 741
SatI GCNGC 3 cut(s) 402, 725, 1352
Sau3AI GATC 5 cut(s) 423, 554, 655, 924, 1216
Sau96I GGNCC 3 cut(s) 672, 1109, 1243
ScrFI CCNGG 1 cut(s) 45
SduI GDGCHC 4 cut(s) 44, 641, 981, 1164
SfaNI GCATC 4 cut(s) 299, 341, 711, 816
SfoI GGCGCC 2 cut(s) 1141, 1279
SinI GGWCC 2 cut(s) 672, 1243
SmiMI CAYNNNNRTG 2 cut(s) 255, 812
SpeI ACTAGT 1 cut(s) 545
Sse9I AATT 4 cut(s) 84, 738, 856, 1100
SsiI CCGC 5 cut(s) 896, 1295, 1334, 1349, 1352
SspDI GGCGCC 2 cut(s) 1139, 1277
SspMI CTAG 3 cut(s) 546, 885, 1077
SstI GAGCTC 1 cut(s) 1164
StyD4I CCNGG 1 cut(s) 43
StyI CCWWGG 2 cut(s) 436, 1246
TaaI ACNGT 3 cut(s) 605, 1194, 1228
TaiI ACGT 2 cut(s) 134, 255
TaqI TCGA 7 cut(s) 117, 553, 658, 697, 927, 986, 1363
TasI AATT 4 cut(s) 84, 738, 856, 1100
TatI WGTACW 1 cut(s) 816
TauI GCSGC 1 cut(s) 1354
TfiI GAWTC 5 cut(s) 101, 122, 1151, 1321, 1327
Tru1I TTAA 2 cut(s) 524, 741
Tru9I TTAA 2 cut(s) 524, 741
TscAI CASTG 4 cut(s) 224, 873, 915, 1011
TseFI GTSAC 4 cut(s) 282, 766, 1127, 1400
TseI GCWGC 2 cut(s) 401, 724
Tsp45I GTSAC 4 cut(s) 282, 766, 1127, 1400
TspDTI ATGAA 6 cut(s) 46, 362, 555, 845, 1134, 1175
TspGWI ACGGA 1 cut(s) 1379
TspRI CASTG 4 cut(s) 224, 873, 915, 1011
Tth111I GACNNNGTC 1 cut(s) 251
VpaK11BI GGWCC 2 cut(s) 672, 1243
XapI RAATTY 1 cut(s) 1100
XcmI CCANNNNNNNNNTGG 1 cut(s) 434
XmnI GAANNNNTTC 1 cut(s) 1054
XspI CTAG 3 cut(s) 546, 885, 1077
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.