Rw5G031770

Wax ester synthase-like Acyl-CoA acyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000634
Physical Location & Seq
Reverse (-)
52741677 .. 52750082
8406 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G031770.1

Sequence Viewer

Length: 1329 bp
ATGGAGTTTGGAGAAGAAGGACTACTATTAGAGCCGGTGAGCCCTGGTGGTCAATTCTTCAACAGCACTGTCTTATCTCTTGCAATTATTTCTGTTTTGGAAATACAAGTTCCAATCAACGATTCTCAAACTTTTTCACTTCTTAAAAATGTCTTCCTCCCGATCAATCCACGCTTCTCTTCCATCATGGTTGAAGGTGTGGATGAAAACAATAAGCAGTGGAAGAGGGTTCAAGTGAATCTGGAAGACCATGTACATGTCCCCATTTTCCCATCCGGAATGTCACTTGAATCATATGATACTTACTTTGATGAATATATAACGAGATATCATGCAGCTGGACAACTAATATTCAAGATCCATCATGCCCTTGGCGATGGCTACTCTCTCATGGGCGCTCTTCTCTCTTGTCTCCAAAATGCTCACAATCCTTCTCTTCCTCTGACATTTCCTTCACTAAAGGGTGCAAAGAGTGAAACTAGTAGTCGATCTCGTGTGTTTGAGTTTGTGCCTAAGATACTTTCTGCCGTTATCAACGGTGCATGGGATTTTAGTTGGAGCATTTTAAAGGGCACTTGGGTTGAAGATGATCGAACACCAATAAGGTCCGGCGTTGATGGAGTTGAGTTTCAGCCTGTGTCCATATCGACCTTGATGCTGTCTATTGAGGAAATCAAACTTATCAAGAACAAGCTTGGAGTGACGATAAATGATGTTATTGCGGGAACAATCTTTCTGGGCACCCGAATGTACATGCAACAGATGAATACTGAAAAATCAAGTAGCCAGAATTGCACGGCATTGGTGTTGCTGAATACTAGACTTGCTGCGGGTTACAAGTCAGTGCAGGAAATGTTGATGGAACCAAGCAAGTCTTCTTGGGGCAACCGATTTGTGTTCTTGCATGTCCCGGTGCCCAAGTCGAGTGAAGTTTCCAAGCCATTGGATTTTGTATGGGAAGCACAGAAGATAGTCAAGAGACATAGAAGCTCTTCAGCTTGGTACCTCACTATTAGGCTCTGGGACATTTTGAAGAAATTTAGAGGCGCTGAGGTTGCAGCTGGATACATTCATAGCACACTCATGAACTCAAGCATGGCAATCACAAATATGATTGGGCCAGTAGAACAAATGGCTTTGGCTGATCACCCCATTAAAGGAGTCTACTTTATGGTGGTCGGTTCACCTCAGAGTCTTACTATAACAGCAATGAGTTATATGGGAAAGCTAAGGATTGCCATGGGAGCTGAAAAAGGCTTTATAGATTCCAACAAATTGCAAGCATGCATGAAAGATGCATTTCGGTTGATATCTGAAGCTGCGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1298 WES_acyltransf

Protein Analysis

442

Amino Acids

49.22

Weight (kDa)

7.16

Isoelectric Point (pI)

46.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WS_DGAT_C PF06974 294 - 437 1e-36 WS/DGAT C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000634)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10290 FvH4_3g28230 FvH4_3g28240
malus_domestica MD05G1036400.v1.1 MD10G1159400.v1.1 MD17G1237100.v1.1 MD17G1237200.v1.1 MD17G1237300.v1.1 MD17G1237600.v1.1 MD17G1238200.v1.1
prunus_persica Prupe.3G140400_v2.0.a1 Prupe.3G140700_v2.0.a1 Prupe.3G141100_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G258100_v2.0.a1 Prupe.7G117300_v2.0.a1 Prupe.7G117400_v2.0.a1 Prupe.7G117600_v2.0.a1 Prupe.8G044700_v2.0.a1
pyrus_communis pycom05g02680 pycom17g24220 pycom17g24240
rosa_chinensis RchiOBHm_Chr1g0350141 RchiOBHm_Chr5g0050931 RchiOBHm_Chr5g0051001 RchiOBHm_Chr5g0051021 RchiOBHm_Chr6g0267491
rosa_laevigata RLG00000013999 RLG00000028502 RLG00000034744 RLG00000034748 RLG00000034753
rosa_multiflora Rmu_sc0000446.1_g000056 Rmu_sc0000782.1_g000006 Rmu_sc0002951.1_g000001 Rmu_sc0003674.1_g000026 Rmu_sc0003756.1_g000004 Rmu_sc0011917.1_g000004 Rmu_sc0012005.1_g000019 Rmu_sc0040739.1_g000001
rosa_roxburghii Rroxscaffold_1G00029730 Rroxscaffold_1G00029740 Rroxscaffold_4G00305060 Rroxscaffold_7G00200710
rosa_rugosa Rorug01G0208400 Rorug05G0265200.1 Rorug05G0265300.1 Rorug05G0265400.1 Rorug05G0265500.1 Rorug05G0294000 Rorug06G0033100 Rorug06G0243300
rosa_samantha Rh1BG192100 Rh1BG192200 Rh1DG220600 Rh5AG270300 Rh5AG337200 Rh5AG337700 Rh5BG346700 Rh5BG347400 Rh5CG374300 Rh5DG359700 Rh5DG360500 Rh6AG154500 Rh6BG155100 Rh6BG362300 Rh6CG150300 Rh6DG140300 Rh6DG356300
rosa_wichuraiana Rw0G018820 Rw1G019350 Rw5G025300 Rw5G031770 Rw5G031820 Rw5G031870 Rw5G031910 Rw6G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1002
AccB1I GGYRCC 3 cut(s) 740, 913, 1002
AccI GTMKAC 1 cut(s) 1164
AccIII TCCGGA 1 cut(s) 275
AciI CCGC 2 cut(s) 722, 830
AclWI GGATC 1 cut(s) 352
AcsI RAATTY 1 cut(s) 1037
AcuI CTGAAG 1 cut(s) 978
AfaI GTAC 3 cut(s) 255, 752, 1004
AfiI CCNNNNNNNGG 1 cut(s) 1157
AflIII ACRYGT 1 cut(s) 256
AgsI TTSAA 7 cut(s) 61, 194, 233, 290, 355, 584, 1033
AhlI ACTAGT 1 cut(s) 479
AjnI CCWGG 1 cut(s) 43
AjuI GAANNNNNNNTTGG 4 cut(s) 106, 138, 859, 891
AluBI AGCT 8 cut(s) 338, 694, 990, 998, 1061, 1228, 1247, 1319
AluI AGCT 8 cut(s) 338, 694, 990, 998, 1061, 1228, 1247, 1319
Alw26I GTCTC 2 cut(s) 416, 973
AlwI GGATC 1 cut(s) 352
Aor13HI TCCGGA 1 cut(s) 275
AoxI GGCC 1 cut(s) 1118
ApeKI GCWGC 4 cut(s) 335, 827, 1058, 1319
ApoI RAATTY 1 cut(s) 1037
Asp700I GAANNNNTTC 1 cut(s) 991
Asp718I GGTACC 1 cut(s) 1002
AspLEI GCGC 2 cut(s) 398, 1049
AspS9I GGNCC 2 cut(s) 606, 1118
AsuC2I CCSGG 1 cut(s) 911
AsuHPI GGTGA 3 cut(s) 49, 1139, 1176
AvaII GGWCC 1 cut(s) 606
BaeGI GKGCMC 3 cut(s) 575, 743, 918
BanI GGYRCC 3 cut(s) 740, 913, 1002
BanII GRGCYC 1 cut(s) 44
BarI GAAGNNNNNNTAC 3 cut(s) 38, 237, 269
BauI CACGAG 1 cut(s) 492
BbsI GAAGAC 3 cut(s) 145, 252, 867
BbvCI CCTCAGC 1 cut(s) 1050
BbvI GCAGC 4 cut(s) 347, 814, 1070, 1306
BccI CCATC 6 cut(s) 191, 280, 369, 371, 611, 853
BceAI ACGGC 2 cut(s) 512, 813
BcgI CGANNNNNNTGC 2 cut(s) 637, 671
BciT130I CCWGG 1 cut(s) 45
BciVI GTATCC 1 cut(s) 1058
BclI TGATCA 1 cut(s) 1144
BcnI CCSGG 1 cut(s) 911
BcoDI GTCTC 2 cut(s) 416, 973
BcuI ACTAGT 1 cut(s) 479
BfaI CTAG 2 cut(s) 480, 819
BfoI RGCGCY 2 cut(s) 399, 1050
BfuI GTATCC 1 cut(s) 1058
BisI GCNGC 4 cut(s) 336, 828, 1059, 1320
BlsI GCNGC 4 cut(s) 337, 829, 1060, 1321
Bme1390I CCNGG 2 cut(s) 45, 911
Bme18I GGWCC 1 cut(s) 606
BmgT120I GGNCC 2 cut(s) 606, 1118
BmiI GGNNCC 4 cut(s) 742, 864, 915, 1004
BmrFI CCNGG 2 cut(s) 45, 911
BmsI GCATC 2 cut(s) 645, 1285
BpiI GAAGAC 3 cut(s) 145, 252, 867
Bpu10I CCTNAGC 2 cut(s) 1050, 1229
BpuEI CTTGAG 1 cut(s) 1075
BpuMI CCSGG 1 cut(s) 911
BsaJI CCNNGG 3 cut(s) 43, 370, 1239
BsaWI WCCGGW 1 cut(s) 275
Bsc4I CCNNNNNNNGG 1 cut(s) 1157
Bse118I RCCGGY 1 cut(s) 34
Bse1I ACTGG 1 cut(s) 1121
Bse3DI GCAATG 1 cut(s) 1215
BseAI TCCGGA 1 cut(s) 275
BseBI CCWGG 1 cut(s) 45
BseDI CCNNGG 3 cut(s) 43, 370, 1239
BseGI GGATG 2 cut(s) 208, 272
BseLI CCNNNNNNNGG 1 cut(s) 1157
BseMI GCAATG 1 cut(s) 1215
BseMII CTCAG 2 cut(s) 1041, 1202
BseNI ACTGG 1 cut(s) 1121
BseSI GKGCMC 3 cut(s) 575, 743, 918
BseXI GCAGC 4 cut(s) 347, 814, 1070, 1306
BsgI GTGCAG 1 cut(s) 866
BshFI GGCC 1 cut(s) 1120
BshNI GGYRCC 3 cut(s) 740, 913, 1002
BsiSI CCGG 4 cut(s) 35, 276, 609, 911
BslFI GGGAC 3 cut(s) 245, 893, 1037
BslI CCNNNNNNNGG 1 cut(s) 1157
BsmAI GTCTC 2 cut(s) 416, 973
BsmFI GGGAC 3 cut(s) 245, 893, 1037
BsnI GGCC 1 cut(s) 1120
Bsp1286I GDGCHC 4 cut(s) 44, 575, 743, 918
Bsp13I TCCGGA 1 cut(s) 275
Bsp1407I TGTACA 2 cut(s) 253, 750
Bsp143I GATC 5 cut(s) 162, 357, 488, 589, 1144
Bsp19I CCATGG 1 cut(s) 1239
BspACI CCGC 2 cut(s) 722, 830
BspANI GGCC 1 cut(s) 1120
BspCNI CTCAG 2 cut(s) 1042, 1201
BspEI TCCGGA 1 cut(s) 275
BspHI TCATGA 1 cut(s) 1083
BspLI GGNNCC 4 cut(s) 742, 864, 915, 1004
BspPI GGATC 1 cut(s) 352
BspQI GCTCTTC 2 cut(s) 405, 997
BspT107I GGYRCC 3 cut(s) 740, 913, 1002
BsrDI GCAATG 1 cut(s) 1215
BsrFI RCCGGY 1 cut(s) 34
BsrGI TGTACA 2 cut(s) 253, 750
BsrI ACTGG 1 cut(s) 1121
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 3 cut(s) 43, 370, 1239
BssMI GATC 5 cut(s) 162, 357, 488, 589, 1144
BssSI CACGAG 1 cut(s) 492
BssT1I CCWWGG 2 cut(s) 370, 1239
Bst2BI CACGAG 1 cut(s) 492
Bst2UI CCWGG 1 cut(s) 45
Bst4CI ACNGT 2 cut(s) 70, 539
Bst6I CTCTTC 5 cut(s) 184, 218, 405, 441, 997
BstAUI TGTACA 2 cut(s) 253, 750
BstC8I GCNNGC 2 cut(s) 1281, 1285
BstDEI CTNAG 4 cut(s) 513, 1050, 1188, 1229
BstDSI CCRYGG 1 cut(s) 1239
BstF5I GGATG 2 cut(s) 208, 272
BstH2I RGCGCY 2 cut(s) 399, 1050
BstHHI GCGC 2 cut(s) 398, 1049
BstKTI GATC 5 cut(s) 165, 360, 491, 592, 1147
BstMAI GTCTC 2 cut(s) 416, 973
BstMBI GATC 5 cut(s) 162, 357, 488, 589, 1144
BstMWI GCNNNNNNNGC 3 cut(s) 792, 1055, 1244
BstNI CCWGG 1 cut(s) 45
BstNSI RCATGY 4 cut(s) 260, 757, 908, 1287
BstSCI CCNGG 2 cut(s) 43, 909
BstSLI GKGCMC 3 cut(s) 575, 743, 918
BstV1I GCAGC 4 cut(s) 347, 814, 1070, 1306
BstV2I GAAGAC 3 cut(s) 145, 252, 867
BstX2I RGATCY 1 cut(s) 357
BstXI CCANNNNNNTGG 1 cut(s) 943
BstYI RGATCY 1 cut(s) 357
BsuI GTATCC 1 cut(s) 1058
BsuRI GGCC 1 cut(s) 1120
BtgI CCRYGG 1 cut(s) 1239
BtgZI GCGATG 1 cut(s) 390
BtsCI GGATG 2 cut(s) 208, 272
BtsI GCAGTG 1 cut(s) 224
BtsIMutI CAGTG 3 cut(s) 66, 224, 849
Cac8I GCNNGC 2 cut(s) 1281, 1285
CciI TCATGA 1 cut(s) 1083
CfoI GCGC 2 cut(s) 398, 1049
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 2 cut(s) 606, 1118
Csp6I GTAC 3 cut(s) 254, 751, 1003
CviQI GTAC 3 cut(s) 254, 751, 1003
DdeI CTNAG 4 cut(s) 513, 1050, 1188, 1229
DpnI GATC 5 cut(s) 164, 359, 490, 591, 1146
DpnII GATC 5 cut(s) 162, 357, 488, 589, 1144
DraI TTTAAA 1 cut(s) 567
Eam1104I CTCTTC 5 cut(s) 184, 218, 405, 441, 997
EarI CTCTTC 5 cut(s) 184, 218, 405, 441, 997
Eco130I CCWWGG 2 cut(s) 370, 1239
Eco24I GRGCYC 1 cut(s) 44
Eco32I GATATC 2 cut(s) 329, 1311
Eco47I GGWCC 1 cut(s) 606
Eco57I CTGAAG 1 cut(s) 978
EcoRII CCWGG 1 cut(s) 43
EcoRV GATATC 2 cut(s) 329, 1311
EcoT14I CCWWGG 2 cut(s) 370, 1239
EcoT22I ATGCAT 2 cut(s) 1289, 1300
EcoT38I GRGCYC 1 cut(s) 44
ErhI CCWWGG 2 cut(s) 370, 1239
FalI AAGNNNNNCTT 2 cut(s) 859, 891
FaqI GGGAC 3 cut(s) 245, 893, 1037
FauI CCCGC 2 cut(s) 715, 823
FauNDI CATATG 1 cut(s) 295
FbaI TGATCA 1 cut(s) 1144
FblI GTMKAC 1 cut(s) 1164
Fnu4HI GCNGC 4 cut(s) 336, 828, 1059, 1320
FokI GGATG 2 cut(s) 215, 259
FriOI GRGCYC 1 cut(s) 44
Fsp4HI GCNGC 4 cut(s) 336, 828, 1059, 1320
FspBI CTAG 2 cut(s) 480, 819
GlaI GCGC 2 cut(s) 397, 1048
GluI GCNGC 4 cut(s) 336, 828, 1059, 1320
HaeII RGCGCY 2 cut(s) 399, 1050
HaeIII GGCC 1 cut(s) 1120
HapII CCGG 4 cut(s) 35, 276, 609, 911
HhaI GCGC 2 cut(s) 398, 1049
Hin6I GCGC 2 cut(s) 396, 1047
HinP1I GCGC 2 cut(s) 396, 1047
HindIII AAGCTT 1 cut(s) 692
HinfI GANTC 6 cut(s) 122, 238, 290, 1161, 1192, 1265
HpaII CCGG 4 cut(s) 35, 276, 609, 911
HphI GGTGA 3 cut(s) 49, 1139, 1176
Hpy166II GTNNAC 2 cut(s) 1165, 1184
Hpy188I TCNGA 3 cut(s) 444, 1191, 1315
Hpy188III TCNNGA 7 cut(s) 160, 242, 276, 355, 685, 976, 1084
Hpy8I GTNNAC 2 cut(s) 1165, 1184
HpyAV CCTTC 4 cut(s) 11, 188, 441, 462
HpyCH4III ACNGT 2 cut(s) 70, 539
HpyF10VI GCNNNNNNNGC 3 cut(s) 792, 1055, 1244
HpyF3I CTNAG 4 cut(s) 513, 1050, 1188, 1229
HspAI GCGC 2 cut(s) 396, 1047
Kpn2I TCCGGA 1 cut(s) 275
KpnI GGTACC 1 cut(s) 1006
Ksp22I TGATCA 1 cut(s) 1144
Kzo9I GATC 5 cut(s) 162, 357, 488, 589, 1144
LguI GCTCTTC 2 cut(s) 405, 997
LmnI GCTCC 2 cut(s) 558, 1244
Lsp1109I GCAGC 4 cut(s) 347, 814, 1070, 1306
LweI GCATC 2 cut(s) 645, 1285
MaeI CTAG 2 cut(s) 480, 819
MaeIII GTNAC 3 cut(s) 282, 700, 833
MalI GATC 5 cut(s) 164, 359, 490, 591, 1146
MboI GATC 5 cut(s) 162, 357, 488, 589, 1144
MflI RGATCY 1 cut(s) 357
MhlI GDGCHC 4 cut(s) 44, 575, 743, 918
MluCI AATT 6 cut(s) 53, 84, 790, 1037, 1274, 1324
MlyI GAGTC 2 cut(s) 1170, 1201
MmeI TCCRAC 2 cut(s) 536, 1293
MnlI CCTC 8 cut(s) 167, 219, 450, 661, 1016, 1037, 1045, 1197
Mph1103I ATGCAT 2 cut(s) 1289, 1300
MroI TCCGGA 1 cut(s) 275
MroXI GAANNNNTTC 1 cut(s) 991
MseI TTAA 4 cut(s) 144, 566, 1155, 1327
MslI CAYNNNNRTG 4 cut(s) 255, 746, 1082, 1109
MspA1I CMGCKG 2 cut(s) 338, 1061
MspI CCGG 4 cut(s) 35, 276, 609, 911
MspR9I CCNGG 2 cut(s) 45, 911
MvaI CCWGG 1 cut(s) 45
MwoI GCNNNNNNNGC 3 cut(s) 792, 1055, 1244
NciI CCSGG 1 cut(s) 911
NcoI CCATGG 1 cut(s) 1239
NdeI CATATG 1 cut(s) 295
NdeII GATC 5 cut(s) 162, 357, 488, 589, 1144
NlaIV GGNNCC 4 cut(s) 742, 864, 915, 1004
NmuCI GTSAC 2 cut(s) 282, 700
NsiI ATGCAT 2 cut(s) 1289, 1300
NspI RCATGY 4 cut(s) 260, 757, 908, 1287
PaeI GCATGC 1 cut(s) 1287
PagI TCATGA 1 cut(s) 1083
PciI ACATGT 1 cut(s) 256
PciSI GCTCTTC 2 cut(s) 405, 997
PdmI GAANNNNTTC 1 cut(s) 991
PfeI GAWTC 4 cut(s) 122, 238, 290, 1265
PkrI GCNGC 4 cut(s) 337, 829, 1060, 1321
PleI GAGTC 2 cut(s) 1169, 1200
PpsI GAGTC 2 cut(s) 1169, 1200
PscI ACATGT 1 cut(s) 256
Psp6I CCWGG 1 cut(s) 43
PspGI CCWGG 1 cut(s) 43
PspN4I GGNNCC 4 cut(s) 742, 864, 915, 1004
PspPI GGNCC 2 cut(s) 606, 1118
PsuI RGATCY 1 cut(s) 357
PvuII CAGCTG 2 cut(s) 338, 1061
RsaI GTAC 3 cut(s) 255, 752, 1004
RsaNI GTAC 3 cut(s) 254, 751, 1003
RseI CAYNNNNRTG 4 cut(s) 255, 746, 1082, 1109
SapI GCTCTTC 2 cut(s) 405, 997
SaqAI TTAA 4 cut(s) 144, 566, 1155, 1327
SatI GCNGC 4 cut(s) 336, 828, 1059, 1320
Sau3AI GATC 5 cut(s) 162, 357, 488, 589, 1144
Sau96I GGNCC 2 cut(s) 606, 1118
SchI GAGTC 2 cut(s) 1170, 1201
ScrFI CCNGG 2 cut(s) 45, 911
SduI GDGCHC 4 cut(s) 44, 575, 743, 918
SfaNI GCATC 2 cut(s) 645, 1285
SinI GGWCC 1 cut(s) 606
SmiMI CAYNNNNRTG 4 cut(s) 255, 746, 1082, 1109
SmlI CTYRAG 1 cut(s) 1090
SmoI CTYRAG 1 cut(s) 1090
SpeI ACTAGT 1 cut(s) 479
SphI GCATGC 1 cut(s) 1287
Sse9I AATT 6 cut(s) 53, 84, 790, 1037, 1274, 1324
SsiI CCGC 2 cut(s) 722, 830
SspI AATATT 1 cut(s) 351
SspMI CTAG 2 cut(s) 480, 819
StyD4I CCNGG 2 cut(s) 43, 909
StyI CCWWGG 2 cut(s) 370, 1239
TaaI ACNGT 2 cut(s) 70, 539
TaqI TCGA 4 cut(s) 487, 592, 647, 923
TasI AATT 6 cut(s) 53, 84, 790, 1037, 1274, 1324
TatI WGTACW 2 cut(s) 253, 750
TfiI GAWTC 4 cut(s) 122, 238, 290, 1265
Tru1I TTAA 4 cut(s) 144, 566, 1155, 1327
Tru9I TTAA 4 cut(s) 144, 566, 1155, 1327
TscAI CASTG 3 cut(s) 73, 224, 849
TseFI GTSAC 2 cut(s) 282, 700
TseI GCWGC 4 cut(s) 335, 827, 1058, 1319
Tsp45I GTSAC 2 cut(s) 282, 700
TspDTI ATGAA 6 cut(s) 219, 327, 779, 1061, 1100, 1304
TspRI CASTG 3 cut(s) 73, 224, 849
VpaK11BI GGWCC 1 cut(s) 606
XapI RAATTY 1 cut(s) 1037
XceI RCATGY 4 cut(s) 260, 757, 908, 1287
XcmI CCANNNNNNNNNTGG 1 cut(s) 368
XmiI GTMKAC 1 cut(s) 1164
XmnI GAANNNNTTC 1 cut(s) 991
XspI CTAG 2 cut(s) 480, 819
Zsp2I ATGCAT 2 cut(s) 1289, 1300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.