Rw5G025300

O-acyltransferase (WSD1-like)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000634
Physical Location & Seq
Forward (+)
36000214 .. 36001680
1467 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G025300.1

Sequence Viewer

Length: 852 bp
ATGCACACACATAGATACACCTACAAGCTGGAATCAACTAAAATGGAGTACTTTGACGAAGGAATACTACAAGAGCCGGTGAGCCCTAGTGGTCAACTCATGAACAGCTCTGTCTTATCTCTTGCAATTCTTGCCGTTTTGGAATCACAAGTTCCAGTCGACGATTCTCAAATTTTTTCAGTTCTTAAAAATGTCTTCCTCCCGATCAATCCACGCTTCTCTTCCATCATGGTTGAATGTGTGGATGAAAAGAATAAGCAGTGGAAGAGGGTTGAAGTGAAGCTGGAAGACCATGTACGTCTTCCCATTTTCCCCTCTGGAAAGTCACTTGAATCATACAATACTTACTTTGATGAGTATATAACAAATATAGCGTCAGAAGCATTCCCCCAAAGTAGGCCATTATGGGAACTTCATCTTTTCAAGTACCCAACAAGTCATGCAGCTGGACAAATAATATTCAAGATCCACCATGCCCTGGGCGATGGCTACTCTCTCATGGGCACTCTTCTCTCTTGTCTCCAAAATGCTCAAAATCCTTCTCTTCCCCTGACATTTCCTTCTCTAAAGGGTGCAAGGAGTGAAACTAGTAGTCGATCTCGTGTGTTTGAGTTTGTGCCTAAGATATTTTCTGCTATTATCAACAGTGCATGGGATTTCAGTTGGAGCATTTTGAAGGGCACTAATTATCTTGAAGATGATCGAACACCAATAAGGTCCGGTGTTGATGGGGTTGAGTTTCAGCCTGTGTCCGTGTCGACCTTGATGCTACCTATTGAGGAAATTAAACTGATCAAGAACAAGCTTGGAGTGGTAGGCTGGTTCGAATATTTTACTTACATACCTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1298 WES_acyltransf

Protein Analysis

283

Amino Acids

32.22

Weight (kDa)

5.83

Isoelectric Point (pI)

53.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WS_DGAT_cat PF03007 72 - 200 1.2e-08 Wax ester synthase/diacylglycerol acyltransferase catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000634)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10290 FvH4_3g28230 FvH4_3g28240
malus_domestica MD05G1036400.v1.1 MD10G1159400.v1.1 MD17G1237100.v1.1 MD17G1237200.v1.1 MD17G1237300.v1.1 MD17G1237600.v1.1 MD17G1238200.v1.1
prunus_persica Prupe.3G140400_v2.0.a1 Prupe.3G140700_v2.0.a1 Prupe.3G141100_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G258100_v2.0.a1 Prupe.7G117300_v2.0.a1 Prupe.7G117400_v2.0.a1 Prupe.7G117600_v2.0.a1 Prupe.8G044700_v2.0.a1
pyrus_communis pycom05g02680 pycom17g24220 pycom17g24240
rosa_chinensis RchiOBHm_Chr1g0350141 RchiOBHm_Chr5g0050931 RchiOBHm_Chr5g0051001 RchiOBHm_Chr5g0051021 RchiOBHm_Chr6g0267491
rosa_laevigata RLG00000013999 RLG00000028502 RLG00000034744 RLG00000034748 RLG00000034753
rosa_multiflora Rmu_sc0000446.1_g000056 Rmu_sc0000782.1_g000006 Rmu_sc0002951.1_g000001 Rmu_sc0003674.1_g000026 Rmu_sc0003756.1_g000004 Rmu_sc0011917.1_g000004 Rmu_sc0012005.1_g000019 Rmu_sc0040739.1_g000001
rosa_roxburghii Rroxscaffold_1G00029730 Rroxscaffold_1G00029740 Rroxscaffold_4G00305060 Rroxscaffold_7G00200710
rosa_rugosa Rorug01G0208400 Rorug05G0265200.1 Rorug05G0265300.1 Rorug05G0265400.1 Rorug05G0265500.1 Rorug05G0294000 Rorug06G0033100 Rorug06G0243300
rosa_samantha Rh1BG192100 Rh1BG192200 Rh1DG220600 Rh5AG270300 Rh5AG337200 Rh5AG337700 Rh5BG346700 Rh5BG347400 Rh5CG374300 Rh5DG359700 Rh5DG360500 Rh6AG154500 Rh6BG155100 Rh6BG362300 Rh6CG150300 Rh6DG140300 Rh6DG356300
rosa_wichuraiana Rw0G018820 Rw1G019350 Rw5G025300 Rw5G031770 Rw5G031820 Rw5G031870 Rw5G031910 Rw6G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 478
AccI GTMKAC 2 cut(s) 159, 758
AclWI GGATC 1 cut(s) 460
AcsI RAATTY 1 cut(s) 171
AfaI GTAC 3 cut(s) 50, 297, 428
AfiI CCNNNNNNNGG 2 cut(s) 396, 478
AgsI TTSAA 7 cut(s) 236, 275, 332, 424, 463, 676, 695
AhlI ACTAGT 1 cut(s) 587
AjnI CCWGG 1 cut(s) 477
AluBI AGCT 5 cut(s) 28, 108, 283, 446, 805
AluI AGCT 5 cut(s) 28, 108, 283, 446, 805
Alw26I GTCTC 1 cut(s) 524
AlwI GGATC 1 cut(s) 460
AoxI GGCC 1 cut(s) 398
ApeKI GCWGC 1 cut(s) 443
ApoI RAATTY 1 cut(s) 171
ArsI GACNNNNNNTTYG 2 cut(s) 359, 391
AspS9I GGNCC 1 cut(s) 717
AsuHPI GGTGA 1 cut(s) 91
AsuII TTCGAA 1 cut(s) 825
AvaII GGWCC 1 cut(s) 717
BaeGI GKGCMC 2 cut(s) 506, 683
BanII GRGCYC 1 cut(s) 86
BarI GAAGNNNNNNTAC 4 cut(s) 51, 83, 279, 311
BauI CACGAG 1 cut(s) 600
BbsI GAAGAC 3 cut(s) 187, 293, 294
BbvI GCAGC 1 cut(s) 455
BccI CCATC 3 cut(s) 233, 479, 722
BceAI ACGGC 1 cut(s) 119
BcgI CGANNNNNNTGC 2 cut(s) 748, 782
BciT130I CCWGG 1 cut(s) 479
BclI TGATCA 1 cut(s) 792
BcoDI GTCTC 1 cut(s) 524
BcuI ACTAGT 1 cut(s) 587
BfaI CTAG 2 cut(s) 87, 588
BisI GCNGC 1 cut(s) 444
BlsI GCNGC 1 cut(s) 445
BmcAI AGTACT 1 cut(s) 50
Bme1390I CCNGG 1 cut(s) 479
Bme18I GGWCC 1 cut(s) 717
BmgT120I GGNCC 1 cut(s) 717
BmrFI CCNGG 1 cut(s) 479
BmsI GCATC 1 cut(s) 756
BpiI GAAGAC 3 cut(s) 187, 293, 294
Bpu14I TTCGAA 1 cut(s) 825
BsaJI CCNNGG 2 cut(s) 477, 478
BsaWI WCCGGW 1 cut(s) 719
Bsc4I CCNNNNNNNGG 2 cut(s) 396, 478
Bse118I RCCGGY 1 cut(s) 76
Bse1I ACTGG 1 cut(s) 155
BseBI CCWGG 1 cut(s) 479
BseDI CCNNGG 2 cut(s) 477, 478
BseGI GGATG 1 cut(s) 250
BseLI CCNNNNNNNGG 2 cut(s) 396, 478
BseNI ACTGG 1 cut(s) 155
BseSI GKGCMC 2 cut(s) 506, 683
BseXI GCAGC 1 cut(s) 455
BshFI GGCC 1 cut(s) 400
BsiSI CCGG 2 cut(s) 77, 720
BslI CCNNNNNNNGG 2 cut(s) 396, 478
BsmAI GTCTC 1 cut(s) 524
BsmI GAATGC 1 cut(s) 383
BsnI GGCC 1 cut(s) 400
Bsp119I TTCGAA 1 cut(s) 825
Bsp1286I GDGCHC 3 cut(s) 86, 506, 683
Bsp143I GATC 5 cut(s) 204, 465, 596, 700, 792
BspANI GGCC 1 cut(s) 400
BspHI TCATGA 1 cut(s) 99
BspPI GGATC 1 cut(s) 460
BspT104I TTCGAA 1 cut(s) 825
BsrFI RCCGGY 1 cut(s) 76
BsrI ACTGG 1 cut(s) 155
BssAI RCCGGY 1 cut(s) 76
BssECI CCNNGG 2 cut(s) 477, 478
BssMI GATC 5 cut(s) 204, 465, 596, 700, 792
BssSI CACGAG 1 cut(s) 600
Bst2BI CACGAG 1 cut(s) 600
Bst2UI CCWGG 1 cut(s) 479
Bst4CI ACNGT 1 cut(s) 647
Bst6I CTCTTC 4 cut(s) 226, 260, 513, 549
BstAPI GCANNNNNTGC 1 cut(s) 131
BstBI TTCGAA 1 cut(s) 825
BstDEI CTNAG 1 cut(s) 621
BstF5I GGATG 1 cut(s) 250
BstKTI GATC 5 cut(s) 207, 468, 599, 703, 795
BstMAI GTCTC 1 cut(s) 524
BstMBI GATC 5 cut(s) 204, 465, 596, 700, 792
BstMWI GCNNNNNNNGC 2 cut(s) 131, 380
BstNI CCWGG 1 cut(s) 479
BstSCI CCNGG 1 cut(s) 477
BstSLI GKGCMC 2 cut(s) 506, 683
BstV1I GCAGC 1 cut(s) 455
BstV2I GAAGAC 3 cut(s) 187, 293, 294
BstX2I RGATCY 1 cut(s) 465
BstYI RGATCY 1 cut(s) 465
BsuRI GGCC 1 cut(s) 400
BtgZI GCGATG 1 cut(s) 498
BtsCI GGATG 1 cut(s) 250
BtsI GCAGTG 1 cut(s) 266
BtsIMutI CAGTG 2 cut(s) 266, 652
CciI TCATGA 1 cut(s) 99
Cfr10I RCCGGY 1 cut(s) 76
Cfr13I GGNCC 1 cut(s) 717
CseI GACGC 1 cut(s) 363
Csp6I GTAC 3 cut(s) 49, 296, 427
CviAII CATG 7 cut(s) 100, 229, 293, 440, 473, 499, 651
CviQI GTAC 3 cut(s) 49, 296, 427
DdeI CTNAG 1 cut(s) 621
DpnI GATC 5 cut(s) 206, 467, 598, 702, 794
DpnII GATC 5 cut(s) 204, 465, 596, 700, 792
Eam1104I CTCTTC 4 cut(s) 226, 260, 513, 549
EarI CTCTTC 4 cut(s) 226, 260, 513, 549
Eco24I GRGCYC 1 cut(s) 86
Eco47I GGWCC 1 cut(s) 717
EcoRII CCWGG 1 cut(s) 477
EcoT38I GRGCYC 1 cut(s) 86
FaeI CATG 7 cut(s) 103, 232, 296, 443, 476, 502, 654
FatI CATG 7 cut(s) 99, 228, 292, 439, 472, 498, 650
FbaI TGATCA 1 cut(s) 792
FblI GTMKAC 2 cut(s) 159, 758
Fnu4HI GCNGC 1 cut(s) 444
FokI GGATG 1 cut(s) 257
FriOI GRGCYC 1 cut(s) 86
Fsp4HI GCNGC 1 cut(s) 444
FspBI CTAG 2 cut(s) 87, 588
GluI GCNGC 1 cut(s) 444
HaeIII GGCC 1 cut(s) 400
HapII CCGG 2 cut(s) 77, 720
HgaI GACGC 1 cut(s) 363
Hin1II CATG 7 cut(s) 103, 232, 296, 443, 476, 502, 654
HincII GTYRAC 3 cut(s) 95, 160, 759
HindII GTYRAC 3 cut(s) 95, 160, 759
HindIII AAGCTT 1 cut(s) 803
HinfI GANTC 4 cut(s) 32, 143, 164, 332
HpaII CCGG 2 cut(s) 77, 720
HphI GGTGA 1 cut(s) 91
Hpy166II GTNNAC 3 cut(s) 95, 160, 759
Hpy188I TCNGA 1 cut(s) 379
Hpy188III TCNNGA 6 cut(s) 100, 202, 318, 463, 692, 796
Hpy8I GTNNAC 3 cut(s) 95, 160, 759
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 4 cut(s) 53, 549, 570, 670
HpyCH4III ACNGT 1 cut(s) 647
HpyCH4IV ACGT 1 cut(s) 298
HpyCH4V TGCA 5 cut(s) 4, 125, 443, 575, 650
HpyF10VI GCNNNNNNNGC 2 cut(s) 131, 380
HpyF3I CTNAG 1 cut(s) 621
HpySE526I ACGT 1 cut(s) 298
Hsp92II CATG 7 cut(s) 103, 232, 296, 443, 476, 502, 654
Ksp22I TGATCA 1 cut(s) 792
Kzo9I GATC 5 cut(s) 204, 465, 596, 700, 792
LmnI GCTCC 1 cut(s) 666
Lsp1109I GCAGC 1 cut(s) 455
LweI GCATC 1 cut(s) 756
MaeI CTAG 2 cut(s) 87, 588
MaeII ACGT 1 cut(s) 298
MaeIII GTNAC 1 cut(s) 324
MalI GATC 5 cut(s) 206, 467, 598, 702, 794
MboI GATC 5 cut(s) 204, 465, 596, 700, 792
MboII GAAGA 8 cut(s) 187, 213, 277, 293, 299, 500, 536, 707
MflI RGATCY 1 cut(s) 465
MhlI GDGCHC 3 cut(s) 86, 506, 683
MluCI AATT 4 cut(s) 126, 171, 685, 783
MmeI TCCRAC 1 cut(s) 644
MnlI CCTC 4 cut(s) 209, 261, 325, 772
MseI TTAA 2 cut(s) 186, 786
MspA1I CMGCKG 1 cut(s) 446
MspI CCGG 2 cut(s) 77, 720
MspR9I CCNGG 1 cut(s) 479
Mva1269I GAATGC 1 cut(s) 383
MvaI CCWGG 1 cut(s) 479
MwoI GCNNNNNNNGC 2 cut(s) 131, 380
NdeII GATC 5 cut(s) 204, 465, 596, 700, 792
NlaIII CATG 7 cut(s) 103, 232, 296, 443, 476, 502, 654
NmuCI GTSAC 1 cut(s) 324
NspV TTCGAA 1 cut(s) 825
PagI TCATGA 1 cut(s) 99
PasI CCCWGGG 1 cut(s) 478
PctI GAATGC 1 cut(s) 383
PfeI GAWTC 4 cut(s) 32, 143, 164, 332
PflMI CCANNNNNTGG 1 cut(s) 478
PkrI GCNGC 1 cut(s) 445
Psp6I CCWGG 1 cut(s) 477
PspGI CCWGG 1 cut(s) 477
PspPI GGNCC 1 cut(s) 717
PsuI RGATCY 1 cut(s) 465
PvuII CAGCTG 1 cut(s) 446
RsaI GTAC 3 cut(s) 50, 297, 428
RsaNI GTAC 3 cut(s) 49, 296, 427
SalI GTCGAC 2 cut(s) 158, 757
SaqAI TTAA 2 cut(s) 186, 786
SatI GCNGC 1 cut(s) 444
Sau3AI GATC 5 cut(s) 204, 465, 596, 700, 792
Sau96I GGNCC 1 cut(s) 717
ScaI AGTACT 1 cut(s) 50
ScrFI CCNGG 1 cut(s) 479
SduI GDGCHC 3 cut(s) 86, 506, 683
SfaNI GCATC 1 cut(s) 756
SfuI TTCGAA 1 cut(s) 825
SinI GGWCC 1 cut(s) 717
SpeI ACTAGT 1 cut(s) 587
Sse9I AATT 4 cut(s) 126, 171, 685, 783
SspI AATATT 2 cut(s) 459, 830
SspMI CTAG 2 cut(s) 87, 588
StyD4I CCNGG 1 cut(s) 477
TaaI ACNGT 1 cut(s) 647
TaiI ACGT 1 cut(s) 301
TaqI TCGA 5 cut(s) 159, 595, 703, 758, 825
TasI AATT 4 cut(s) 126, 171, 685, 783
TatI WGTACW 1 cut(s) 48
TfiI GAWTC 4 cut(s) 32, 143, 164, 332
Tru1I TTAA 2 cut(s) 186, 786
Tru9I TTAA 2 cut(s) 186, 786
TscAI CASTG 2 cut(s) 266, 652
TseFI GTSAC 1 cut(s) 324
TseI GCWGC 1 cut(s) 443
Tsp45I GTSAC 1 cut(s) 324
TspDTI ATGAA 3 cut(s) 116, 261, 404
TspGWI ACGGA 1 cut(s) 742
TspRI CASTG 2 cut(s) 266, 652
Van91I CCANNNNNTGG 1 cut(s) 478
VpaK11BI GGWCC 1 cut(s) 717
XapI RAATTY 1 cut(s) 171
XmiI GTMKAC 2 cut(s) 159, 758
XspI CTAG 2 cut(s) 87, 588
ZrmI AGTACT 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.