Rw5G031820

O-acyltransferase (WSD1-like)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000634
Physical Location & Seq
Reverse (-)
53009047 .. 53031865
22819 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G031820.1

Sequence Viewer

Length: 1350 bp
ATGGTGTTTGGCGAAGGAGGACTACCACAAGAGCCGGTGAGCCCGGGAAGTCAATTACTCAACAGCTCAGTCTTATCTCTTGCAATTCTTGCCGTTTTGGAATCACAAGTTCCAGTCGACGATTCTCAAACTTTTTTGCTCCTTAAAAATGTCTTCCTCCCGATTAATCCACGCTTCTCTTCCATCATGGTTGAAGGTGTCGATGAAAAGAATAAGCAGTGGAAGAGGGTTGAAGTGAAGCTGGAAGACCATGTGCATTATATAACGAAGATAGCATCAGAAACATTCCCACAAAGCAGGCCATTGTGGGAACTTCATCTTTTCAAGTACCCAACAAGTCATGCGGCTGGACAGATAATATTCAAGATACACCATGCCCTTGGCGATGGCTACTCTCTCATGGGTGCCCTTCTCTCTTGTCTCCAAAATGCTCAAAATCCTTCTCTTCCCCTGACATTTCCTTCACTAAAGGGTGCAAAGAATGAAACTAATAGTCGATCTCGTGTGTTTGAGTTTGTGCCTAAGATATTTTCTGCCGTTATCAACGGTGCATGGGATTTATGTTGGAGCATTTTGAAGGGCACTTGGGTTGAAGATGATCGAACACCAATAAGGTCCGGTGTTGATGGAGTTGAGTTTCGACCTTTGTCCGTATCGACCTTGATGCTGCCTGTTGAGGAAATTAAACTTATCAAGAACAAGCTTGGAGTGACGATAAATGATGTGATTTCAGGAACAATCTTTCTGAGCATCCGAATGTACATTCAAGAGATGGATAATGAAAAATCAAGTAGCCAGAATTGCACCGCATTGGTGTTGCTGAATAGTAGACTTGCTGCGGGTTACAAGTCAGTGCAGGAGATGTTGATCGAAACAAACAAGTCTTGGGGAAACCGAATTGTGTTCTTGCCCGTCTCGGTGCCCAAGTCAAGTGAAGTTACAAAGCCACTGGATTTTGTGTGGGAAGCACGTAATATAATCAAGAGACAGAGAAACTCTTCATCTTGGTACCTCACCACTAGGCTCTTGGATATTTTGAAGAAATTTAGAGGCCCTGAGGTAGTATCTAAATATATCTATAATACACTCAAAAACTCAAGCACAGGAATCACAAATATGATTGGACCATTGGAACAAATGAGTATCGCCAATCACCCCATTAAAGGAATTTACTTTATAGTGACCGGTACACCTCAGTGTCTTGCTATAACAGCAATGAGTTATATGGGAAAGCTAAGGATTGCCTTCGGAGCTGAAAAAGGCTTCATAGATACCAACGTATTGCAGGCATGCATGAAAGATGCCTTTCGGGTGATATGTGAAGCTGCGAATGAATTTCCTATCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1298 WES_acyltransf

Protein Analysis

449

Amino Acids

50.18

Weight (kDa)

8.94

Isoelectric Point (pI)

43.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WS_DGAT_cat PF03007 87 - 243 9.4e-09 Wax ester synthase/diacylglycerol acyltransferase catalytic domain
WS_DGAT_C PF06974 296 - 439 6.9e-35 WS/DGAT C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000634)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10290 FvH4_3g28230 FvH4_3g28240
malus_domestica MD05G1036400.v1.1 MD10G1159400.v1.1 MD17G1237100.v1.1 MD17G1237200.v1.1 MD17G1237300.v1.1 MD17G1237600.v1.1 MD17G1238200.v1.1
prunus_persica Prupe.3G140400_v2.0.a1 Prupe.3G140700_v2.0.a1 Prupe.3G141100_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G258100_v2.0.a1 Prupe.7G117300_v2.0.a1 Prupe.7G117400_v2.0.a1 Prupe.7G117600_v2.0.a1 Prupe.8G044700_v2.0.a1
pyrus_communis pycom05g02680 pycom17g24220 pycom17g24240
rosa_chinensis RchiOBHm_Chr1g0350141 RchiOBHm_Chr5g0050931 RchiOBHm_Chr5g0051001 RchiOBHm_Chr5g0051021 RchiOBHm_Chr6g0267491
rosa_laevigata RLG00000013999 RLG00000028502 RLG00000034744 RLG00000034748 RLG00000034753
rosa_multiflora Rmu_sc0000446.1_g000056 Rmu_sc0000782.1_g000006 Rmu_sc0002951.1_g000001 Rmu_sc0003674.1_g000026 Rmu_sc0003756.1_g000004 Rmu_sc0011917.1_g000004 Rmu_sc0012005.1_g000019 Rmu_sc0040739.1_g000001
rosa_roxburghii Rroxscaffold_1G00029730 Rroxscaffold_1G00029740 Rroxscaffold_4G00305060 Rroxscaffold_7G00200710
rosa_rugosa Rorug01G0208400 Rorug05G0265200.1 Rorug05G0265300.1 Rorug05G0265400.1 Rorug05G0265500.1 Rorug05G0294000 Rorug06G0033100 Rorug06G0243300
rosa_samantha Rh1BG192100 Rh1BG192200 Rh1DG220600 Rh5AG270300 Rh5AG337200 Rh5AG337700 Rh5BG346700 Rh5BG347400 Rh5CG374300 Rh5DG359700 Rh5DG360500 Rh6AG154500 Rh6BG155100 Rh6BG362300 Rh6CG150300 Rh6DG140300 Rh6DG356300
rosa_wichuraiana Rw0G018820 Rw1G019350 Rw5G025300 Rw5G031770 Rw5G031820 Rw5G031870 Rw5G031910 Rw6G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1008
AccB1I GGYRCC 3 cut(s) 404, 919, 1008
AccI GTMKAC 2 cut(s) 117, 829
AciI CCGC 3 cut(s) 344, 807, 839
AcsI RAATTY 3 cut(s) 1043, 1167, 1334
AfaI GTAC 4 cut(s) 329, 761, 1010, 1189
AfiI CCNNNNNNNGG 1 cut(s) 1163
AgeI ACCGGT 1 cut(s) 1184
AgsI TTSAA 8 cut(s) 194, 233, 325, 364, 577, 593, 767, 1039
AleI CACNNNNGTG 1 cut(s) 1195
AluBI AGCT 6 cut(s) 66, 241, 703, 1234, 1253, 1325
AluI AGCT 6 cut(s) 66, 241, 703, 1234, 1253, 1325
Alw26I GTCTC 3 cut(s) 425, 919, 979
Ama87I CYCGRG 1 cut(s) 43
AoxI GGCC 2 cut(s) 299, 1051
ApeKI GCWGC 3 cut(s) 667, 836, 1325
ApoI RAATTY 3 cut(s) 1043, 1167, 1334
AseI ATTAAT 1 cut(s) 165
AsiGI ACCGGT 1 cut(s) 1184
Asp700I GAANNNNTTC 1 cut(s) 997
Asp718I GGTACC 1 cut(s) 1008
AspS9I GGNCC 3 cut(s) 615, 1052, 1124
AsuC2I CCSGG 2 cut(s) 44, 45
AsuHPI GGTGA 4 cut(s) 49, 1006, 1145, 1324
AvaI CYCGRG 1 cut(s) 43
AvaII GGWCC 2 cut(s) 615, 1124
AxyI CCTNAGG 1 cut(s) 1056
BaeGI GKGCMC 3 cut(s) 409, 584, 924
BaeI ACNNNNGTAYC 2 cut(s) 1263, 1296
BanI GGYRCC 3 cut(s) 404, 919, 1008
BanII GRGCYC 1 cut(s) 44
BarI GAAGNNNNNNTAC 1 cut(s) 38
BauI CACGAG 1 cut(s) 501
BbsI GAAGAC 2 cut(s) 145, 252
BbvI GCAGC 3 cut(s) 654, 823, 1312
BccI CCATC 4 cut(s) 191, 380, 620, 766
BceAI ACGGC 2 cut(s) 77, 521
BcgI CGANNNNNNTGC 2 cut(s) 646, 680
BcnI CCSGG 2 cut(s) 44, 45
BcoDI GTCTC 3 cut(s) 425, 919, 979
BfaI CTAG 1 cut(s) 1020
BisI GCNGC 4 cut(s) 345, 668, 837, 1326
BlsI GCNGC 4 cut(s) 346, 669, 838, 1327
Bme1390I CCNGG 2 cut(s) 44, 45
Bme18I GGWCC 2 cut(s) 615, 1124
BmeT110I CYCGRG 1 cut(s) 43
BmgT120I GGNCC 3 cut(s) 615, 1052, 1124
BmiI GGNNCC 3 cut(s) 406, 921, 1010
BmrFI CCNGG 2 cut(s) 44, 45
BmsI GCATC 4 cut(s) 284, 654, 759, 1291
BoxI GACNNNNGTC 1 cut(s) 646
BpiI GAAGAC 2 cut(s) 145, 252
Bpu10I CCTNAGC 1 cut(s) 1235
BpuEI CTTGAG 1 cut(s) 1081
BpuMI CCSGG 2 cut(s) 44, 45
BsaAI YACGTR 1 cut(s) 971
BsaBI GATNNNNATC 1 cut(s) 866
BsaJI CCNNGG 2 cut(s) 43, 379
BsaWI WCCGGW 2 cut(s) 617, 1184
Bsc4I CCNNNNNNNGG 1 cut(s) 1163
Bse118I RCCGGY 2 cut(s) 34, 1184
Bse1I ACTGG 2 cut(s) 113, 954
Bse21I CCTNAGG 1 cut(s) 1056
Bse3DI GCAATG 1 cut(s) 1221
Bse8I GATNNNNATC 1 cut(s) 866
BseDI CCNNGG 2 cut(s) 43, 379
BseGI GGATG 1 cut(s) 750
BseJI GATNNNNATC 1 cut(s) 866
BseLI CCNNNNNNNGG 1 cut(s) 1163
BseMI GCAATG 1 cut(s) 1221
BseMII CTCAG 4 cut(s) 81, 737, 1047, 1208
BseNI ACTGG 2 cut(s) 113, 954
BseSI GKGCMC 3 cut(s) 409, 584, 924
BseXI GCAGC 3 cut(s) 654, 823, 1312
BsgI GTGCAG 1 cut(s) 875
BshFI GGCC 2 cut(s) 301, 1053
BshNI GGYRCC 3 cut(s) 404, 919, 1008
BshTI ACCGGT 1 cut(s) 1184
BsiHKCI CYCGRG 1 cut(s) 43
BsiSI CCGG 4 cut(s) 35, 44, 618, 1185
BslI CCNNNNNNNGG 1 cut(s) 1163
BsmAI GTCTC 3 cut(s) 425, 919, 979
BsmBI CGTCTC 1 cut(s) 919
BsnI GGCC 2 cut(s) 301, 1053
BsoBI CYCGRG 1 cut(s) 43
Bsp1286I GDGCHC 4 cut(s) 44, 409, 584, 924
Bsp1407I TGTACA 1 cut(s) 759
Bsp143I GATC 3 cut(s) 497, 598, 867
BspACI CCGC 3 cut(s) 344, 807, 839
BspANI GGCC 2 cut(s) 301, 1053
BspCNI CTCAG 4 cut(s) 80, 738, 1048, 1207
BspLI GGNNCC 3 cut(s) 406, 921, 1010
BspT107I GGYRCC 3 cut(s) 404, 919, 1008
BsrDI GCAATG 1 cut(s) 1221
BsrFI RCCGGY 2 cut(s) 34, 1184
BsrGI TGTACA 1 cut(s) 759
BsrI ACTGG 2 cut(s) 113, 954
BssAI RCCGGY 2 cut(s) 34, 1184
BssECI CCNNGG 2 cut(s) 43, 379
BssMI GATC 3 cut(s) 497, 598, 867
BssSI CACGAG 1 cut(s) 501
BssT1I CCWWGG 1 cut(s) 379
Bst2BI CACGAG 1 cut(s) 501
Bst4CI ACNGT 1 cut(s) 548
Bst6I CTCTTC 4 cut(s) 184, 218, 450, 1003
BstAPI GCANNNNNTGC 1 cut(s) 89
BstAUI TGTACA 1 cut(s) 759
BstBAI YACGTR 1 cut(s) 971
BstC8I GCNNGC 3 cut(s) 299, 1287, 1291
BstDEI CTNAG 6 cut(s) 67, 522, 746, 1056, 1194, 1235
BstF5I GGATG 1 cut(s) 750
BstKTI GATC 3 cut(s) 500, 601, 870
BstMAI GTCTC 3 cut(s) 425, 919, 979
BstMBI GATC 3 cut(s) 497, 598, 867
BstMWI GCNNNNNNNGC 4 cut(s) 89, 801, 1211, 1250
BstNSI RCATGY 1 cut(s) 1293
BstPAI GACNNNNGTC 1 cut(s) 646
BstSCI CCNGG 2 cut(s) 42, 43
BstSLI GKGCMC 3 cut(s) 409, 584, 924
BstV1I GCAGC 3 cut(s) 654, 823, 1312
BstV2I GAAGAC 2 cut(s) 145, 252
BstXI CCANNNNNNTGG 1 cut(s) 380
Bsu36I CCTNAGG 1 cut(s) 1056
BsuRI GGCC 2 cut(s) 301, 1053
BtgZI GCGATG 1 cut(s) 399
BtsCI GGATG 1 cut(s) 750
BtsI GCAGTG 1 cut(s) 224
BtsIMutI CAGTG 4 cut(s) 224, 858, 947, 1202
Cac8I GCNNGC 3 cut(s) 299, 1287, 1291
Cfr10I RCCGGY 2 cut(s) 34, 1184
Cfr13I GGNCC 3 cut(s) 615, 1052, 1124
Cfr9I CCCGGG 1 cut(s) 43
Csp6I GTAC 4 cut(s) 328, 760, 1009, 1188
CspAI ACCGGT 1 cut(s) 1184
CviAII CATG 8 cut(s) 187, 251, 341, 374, 400, 552, 1290, 1294
CviQI GTAC 4 cut(s) 328, 760, 1009, 1188
DdeI CTNAG 6 cut(s) 67, 522, 746, 1056, 1194, 1235
DpnI GATC 3 cut(s) 499, 600, 869
DpnII GATC 3 cut(s) 497, 598, 867
Eam1104I CTCTTC 4 cut(s) 184, 218, 450, 1003
EarI CTCTTC 4 cut(s) 184, 218, 450, 1003
Eco130I CCWWGG 1 cut(s) 379
Eco24I GRGCYC 1 cut(s) 44
Eco47I GGWCC 2 cut(s) 615, 1124
Eco81I CCTNAGG 1 cut(s) 1056
Eco88I CYCGRG 1 cut(s) 43
EcoO109I RGGNCCY 1 cut(s) 1052
EcoT14I CCWWGG 1 cut(s) 379
EcoT22I ATGCAT 1 cut(s) 1295
EcoT38I GRGCYC 1 cut(s) 44
ErhI CCWWGG 1 cut(s) 379
Esp3I CGTCTC 1 cut(s) 919
FaeI CATG 8 cut(s) 190, 254, 344, 377, 403, 555, 1293, 1297
FatI CATG 8 cut(s) 186, 250, 340, 373, 399, 551, 1289, 1293
FauI CCCGC 1 cut(s) 832
FblI GTMKAC 2 cut(s) 117, 829
Fnu4HI GCNGC 4 cut(s) 345, 668, 837, 1326
FokI GGATG 1 cut(s) 737
FriOI GRGCYC 1 cut(s) 44
Fsp4HI GCNGC 4 cut(s) 345, 668, 837, 1326
FspBI CTAG 1 cut(s) 1020
GluI GCNGC 4 cut(s) 345, 668, 837, 1326
HaeIII GGCC 2 cut(s) 301, 1053
HapII CCGG 4 cut(s) 35, 44, 618, 1185
Hin1II CATG 8 cut(s) 190, 254, 344, 377, 403, 555, 1293, 1297
HincII GTYRAC 1 cut(s) 118
HindII GTYRAC 1 cut(s) 118
HindIII AAGCTT 1 cut(s) 701
HinfI GANTC 3 cut(s) 101, 122, 1107
HpaII CCGG 4 cut(s) 35, 44, 618, 1185
HphI GGTGA 4 cut(s) 49, 1006, 1145, 1324
Hpy166II GTNNAC 3 cut(s) 118, 830, 1190
Hpy188I TCNGA 4 cut(s) 280, 747, 755, 1250
Hpy188III TCNNGA 6 cut(s) 160, 364, 694, 732, 767, 982
Hpy8I GTNNAC 3 cut(s) 118, 830, 1190
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 7 cut(s) 8, 188, 419, 450, 471, 571, 1255
HpyCH4III ACNGT 1 cut(s) 548
HpyCH4IV ACGT 2 cut(s) 970, 1278
HpyCH4V TGCA 8 cut(s) 83, 256, 476, 551, 804, 856, 1285, 1293
HpyF10VI GCNNNNNNNGC 4 cut(s) 89, 801, 1211, 1250
HpyF3I CTNAG 6 cut(s) 67, 522, 746, 1056, 1194, 1235
HpySE526I ACGT 2 cut(s) 970, 1278
Hsp92II CATG 8 cut(s) 190, 254, 344, 377, 403, 555, 1293, 1297
KpnI GGTACC 1 cut(s) 1012
Kzo9I GATC 3 cut(s) 497, 598, 867
LmnI GCTCC 3 cut(s) 144, 567, 1250
Lsp1109I GCAGC 3 cut(s) 654, 823, 1312
LweI GCATC 4 cut(s) 284, 654, 759, 1291
MaeI CTAG 1 cut(s) 1020
MaeII ACGT 2 cut(s) 970, 1278
MaeIII GTNAC 4 cut(s) 709, 842, 937, 1180
MalI GATC 3 cut(s) 499, 600, 869
MboI GATC 3 cut(s) 497, 598, 867
MboII GAAGA 9 cut(s) 145, 171, 235, 257, 280, 437, 605, 990, 1051
MhlI GDGCHC 4 cut(s) 44, 409, 584, 924
MluCI AATT 8 cut(s) 53, 84, 681, 799, 897, 1043, 1167, 1334
MmeI TCCRAC 1 cut(s) 545
MnlI CCTC 8 cut(s) 11, 167, 219, 670, 1022, 1043, 1051, 1203
Mph1103I ATGCAT 1 cut(s) 1295
MroXI GAANNNNTTC 1 cut(s) 997
MseI TTAA 4 cut(s) 144, 165, 684, 1161
MslI CAYNNNNRTG 3 cut(s) 755, 1115, 1195
MspI CCGG 4 cut(s) 35, 44, 618, 1185
MspR9I CCNGG 2 cut(s) 44, 45
MwoI GCNNNNNNNGC 4 cut(s) 89, 801, 1211, 1250
NciI CCSGG 2 cut(s) 44, 45
NdeII GATC 3 cut(s) 497, 598, 867
NlaIII CATG 8 cut(s) 190, 254, 344, 377, 403, 555, 1293, 1297
NlaIV GGNNCC 3 cut(s) 406, 921, 1010
NmuCI GTSAC 2 cut(s) 709, 1180
NsiI ATGCAT 1 cut(s) 1295
NspI RCATGY 1 cut(s) 1293
OliI CACNNNNGTG 1 cut(s) 1195
PaeI GCATGC 1 cut(s) 1293
PdmI GAANNNNTTC 1 cut(s) 997
PfeI GAWTC 3 cut(s) 101, 122, 1107
PinAI ACCGGT 1 cut(s) 1184
PkrI GCNGC 4 cut(s) 346, 669, 838, 1327
Ppu21I YACGTR 1 cut(s) 971
PshAI GACNNNNGTC 1 cut(s) 646
PshBI ATTAAT 1 cut(s) 165
PspN4I GGNNCC 3 cut(s) 406, 921, 1010
PspPI GGNCC 3 cut(s) 615, 1052, 1124
RsaI GTAC 4 cut(s) 329, 761, 1010, 1189
RsaNI GTAC 4 cut(s) 328, 760, 1009, 1188
RseI CAYNNNNRTG 3 cut(s) 755, 1115, 1195
SalI GTCGAC 1 cut(s) 116
SaqAI TTAA 4 cut(s) 144, 165, 684, 1161
SatI GCNGC 4 cut(s) 345, 668, 837, 1326
Sau3AI GATC 3 cut(s) 497, 598, 867
Sau96I GGNCC 3 cut(s) 615, 1052, 1124
ScrFI CCNGG 2 cut(s) 44, 45
SduI GDGCHC 4 cut(s) 44, 409, 584, 924
SfaNI GCATC 4 cut(s) 284, 654, 759, 1291
SinI GGWCC 2 cut(s) 615, 1124
SmaI CCCGGG 1 cut(s) 45
SmiMI CAYNNNNRTG 3 cut(s) 755, 1115, 1195
SmlI CTYRAG 1 cut(s) 1096
SmoI CTYRAG 1 cut(s) 1096
SphI GCATGC 1 cut(s) 1293
Sse9I AATT 8 cut(s) 53, 84, 681, 799, 897, 1043, 1167, 1334
SsiI CCGC 3 cut(s) 344, 807, 839
SspI AATATT 1 cut(s) 360
SspMI CTAG 1 cut(s) 1020
StyD4I CCNGG 2 cut(s) 42, 43
StyI CCWWGG 1 cut(s) 379
TaaI ACNGT 1 cut(s) 548
TaiI ACGT 2 cut(s) 973, 1281
TaqI TCGA 7 cut(s) 117, 201, 496, 601, 640, 656, 870
TasI AATT 8 cut(s) 53, 84, 681, 799, 897, 1043, 1167, 1334
TatI WGTACW 1 cut(s) 759
TauI GCSGC 1 cut(s) 347
TfiI GAWTC 3 cut(s) 101, 122, 1107
Tru1I TTAA 4 cut(s) 144, 165, 684, 1161
Tru9I TTAA 4 cut(s) 144, 165, 684, 1161
TscAI CASTG 4 cut(s) 224, 858, 954, 1202
TseFI GTSAC 2 cut(s) 709, 1180
TseI GCWGC 3 cut(s) 667, 836, 1325
Tsp45I GTSAC 2 cut(s) 709, 1180
TspDTI ATGAA 8 cut(s) 219, 305, 498, 795, 990, 1255, 1310, 1347
TspGWI ACGGA 1 cut(s) 640
TspMI CCCGGG 1 cut(s) 43
TspRI CASTG 4 cut(s) 224, 858, 954, 1202
VpaK11BI GGWCC 2 cut(s) 615, 1124
VspI ATTAAT 1 cut(s) 165
XapI RAATTY 3 cut(s) 1043, 1167, 1334
XceI RCATGY 1 cut(s) 1293
XcmI CCANNNNNNNNNTGG 1 cut(s) 1024
XmaI CCCGGG 1 cut(s) 43
XmiI GTMKAC 2 cut(s) 117, 829
XmnI GAANNNNTTC 1 cut(s) 997
XspI CTAG 1 cut(s) 1020
Zsp2I ATGCAT 1 cut(s) 1295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.