Rmu_sc0003674.1_g000026

Wax ester synthase-like Acyl-CoA acyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003674.1
OG0000634
Physical Location & Seq
Forward (+)
150861 .. 152544
1684 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003674.1_g000026.1.cds

Sequence Viewer

Length: 1134 bp
atggagtttggagaagaaggattactactggagccggtgagccctggtggtcaattcttcaacagtactgctttatctcttgcaattatttccgttatggaatcacaagtttcaattaacgatactcaaactttttcacttcttaaaaatgtgttcctcccgatcaatccacgcttctcttccatcatggttgaaggtgtggatggaaagaataagcagtggaagagggttcaagtgaagttggaagaccacgtgcatgtccccattttcccctctggaatgtcacttgaatcatacgatacttactttgatgagtatatgacgaagatagcatcagaaacattcccacaaagcaggccattgtgggaacttcatcttttcaagtacccaacaagtcatgcagctggacaaataatattcaagatccaccatgcccttggcgatggctactctctcatgggcgctcttctctcttgtctccaaaatgctcagaatccttctcttcccctgacatttccttcactaaagggtgcaaagaatgaaactagtagtcgatctcgtgtatttaagtttgtgcctaagatattttctgctgttatcaacggtgcatgggatttcggttggagcattttgaagggcacttgggttgaagatgatcgaacaccaataaggtcaggcgttgttggagttgagtttcggcctgtgtccacgtcaaccttgatgctgtctattgaggaaatcaaacttatcaagaacaaccttggggtgacgataaatgatgttattgcgggaataatctttctaggcacccgaatgtacatgcaacagatgaatagtgaaaaatcaagtagccagaattgcacagcactcgtgttgctgaacactagacttgttgcgaattacaagtcggtgcaggagatgttgatggaaccaagcaagtcttcttggggcaaccaatttgtgttcttgcatgtcccggtgcccaagtcgagtgaagtttccaagccattggattttgtatgggaagcacataaaatagtcaagagacatagaagctcttcagcttggtaccttactattaggctctgggacattttgaagaaatttagaggccctgaggtaactaattattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1298 WES_acyltransf

Protein Analysis

377

Amino Acids

42.54

Weight (kDa)

8.85

Isoelectric Point (pI)

44.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000634)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10290 FvH4_3g28230 FvH4_3g28240
malus_domestica MD05G1036400.v1.1 MD10G1159400.v1.1 MD17G1237100.v1.1 MD17G1237200.v1.1 MD17G1237300.v1.1 MD17G1237600.v1.1 MD17G1238200.v1.1
prunus_persica Prupe.3G140400_v2.0.a1 Prupe.3G140700_v2.0.a1 Prupe.3G141100_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G258100_v2.0.a1 Prupe.7G117300_v2.0.a1 Prupe.7G117400_v2.0.a1 Prupe.7G117600_v2.0.a1 Prupe.8G044700_v2.0.a1
pyrus_communis pycom05g02680 pycom17g24220 pycom17g24240
rosa_chinensis RchiOBHm_Chr1g0350141 RchiOBHm_Chr5g0050931 RchiOBHm_Chr5g0051001 RchiOBHm_Chr5g0051021 RchiOBHm_Chr6g0267491
rosa_laevigata RLG00000013999 RLG00000028502 RLG00000034744 RLG00000034748 RLG00000034753
rosa_multiflora Rmu_sc0000446.1_g000056 Rmu_sc0000782.1_g000006 Rmu_sc0002951.1_g000001 Rmu_sc0003674.1_g000026 Rmu_sc0003756.1_g000004 Rmu_sc0011917.1_g000004 Rmu_sc0012005.1_g000019 Rmu_sc0040739.1_g000001
rosa_roxburghii Rroxscaffold_1G00029730 Rroxscaffold_1G00029740 Rroxscaffold_4G00305060 Rroxscaffold_7G00200710
rosa_rugosa Rorug01G0208400 Rorug05G0265200.1 Rorug05G0265300.1 Rorug05G0265400.1 Rorug05G0265500.1 Rorug05G0294000 Rorug06G0033100 Rorug06G0243300
rosa_samantha Rh1BG192100 Rh1BG192200 Rh1DG220600 Rh5AG270300 Rh5AG337200 Rh5AG337700 Rh5BG346700 Rh5BG347400 Rh5CG374300 Rh5DG359700 Rh5DG360500 Rh6AG154500 Rh6BG155100 Rh6BG362300 Rh6CG150300 Rh6DG140300 Rh6DG356300
rosa_wichuraiana Rw0G018820 Rw1G019350 Rw5G025300 Rw5G031770 Rw5G031820 Rw5G031870 Rw5G031910 Rw6G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1068
AccB1I GGYRCC 3 cut(s) 806, 979, 1068
AciI CCGC 1 cut(s) 788
AclWI GGATC 1 cut(s) 418
AcsI RAATTY 1 cut(s) 1103
AcuI CTGAAG 1 cut(s) 1044
AcvI CACGTG 1 cut(s) 253
AfaI GTAC 4 cut(s) 67, 386, 818, 1070
AhlI ACTAGT 1 cut(s) 545
AjiI CACGTC 1 cut(s) 711
AjnI CCWGG 1 cut(s) 43
AjuI GAANNNNNNNTTGG 2 cut(s) 925, 957
AluBI AGCT 3 cut(s) 404, 1056, 1064
AluI AGCT 3 cut(s) 404, 1056, 1064
Alw26I GTCTC 2 cut(s) 482, 1039
AlwI GGATC 1 cut(s) 418
AoxI GGCC 3 cut(s) 356, 698, 1111
ApeKI GCWGC 1 cut(s) 401
ApoI RAATTY 1 cut(s) 1103
ArsI GACNNNNNNTTYG 2 cut(s) 890, 922
Asp700I GAANNNNTTC 1 cut(s) 1057
Asp718I GGTACC 1 cut(s) 1068
AspLEI GCGC 1 cut(s) 464
AspS9I GGNCC 1 cut(s) 1112
AsuC2I CCSGG 1 cut(s) 977
AsuHPI GGTGA 2 cut(s) 49, 778
AxyI CCTNAGG 1 cut(s) 1116
BaeGI GKGCMC 2 cut(s) 641, 984
BanI GGYRCC 3 cut(s) 806, 979, 1068
BanII GRGCYC 1 cut(s) 44
BarI GAAGNNNNNNTAC 3 cut(s) 9, 38, 41
BauI CACGAG 2 cut(s) 558, 869
BbrPI CACGTG 1 cut(s) 253
BbsI GAAGAC 2 cut(s) 252, 933
BbvI GCAGC 1 cut(s) 413
BccI CCATC 4 cut(s) 191, 197, 437, 919
BcgI CGANNNNNNTGC 2 cut(s) 850, 884
BciT130I CCWGG 1 cut(s) 45
BcnI CCSGG 1 cut(s) 977
BcoDI GTCTC 2 cut(s) 482, 1039
BcuI ACTAGT 1 cut(s) 545
BfaI CTAG 3 cut(s) 546, 803, 885
BfoI RGCGCY 1 cut(s) 465
BisI GCNGC 1 cut(s) 402
BlsI GCNGC 1 cut(s) 403
BmcAI AGTACT 1 cut(s) 67
Bme1390I CCNGG 2 cut(s) 45, 977
BmgBI CACGTC 1 cut(s) 711
BmgT120I GGNCC 1 cut(s) 1112
BmiI GGNNCC 5 cut(s) 33, 808, 930, 981, 1070
BmrFI CCNGG 2 cut(s) 45, 977
BmsI GCATC 2 cut(s) 341, 711
BpiI GAAGAC 2 cut(s) 252, 933
BpmI CTGGAG 1 cut(s) 50
BpuMI CCSGG 1 cut(s) 977
BsaAI YACGTR 1 cut(s) 253
BsaJI CCNNGG 3 cut(s) 43, 436, 760
Bse118I RCCGGY 1 cut(s) 34
Bse1I ACTGG 1 cut(s) 33
Bse21I CCTNAGG 1 cut(s) 1116
BseBI CCWGG 1 cut(s) 45
BseDI CCNNGG 3 cut(s) 43, 436, 760
BseGI GGATG 1 cut(s) 208
BseMII CTCAG 2 cut(s) 503, 1107
BseNI ACTGG 1 cut(s) 33
BseSI GKGCMC 2 cut(s) 641, 984
BseXI GCAGC 1 cut(s) 413
BsgI GTGCAG 1 cut(s) 932
BshFI GGCC 3 cut(s) 358, 700, 1113
BshNI GGYRCC 3 cut(s) 806, 979, 1068
BsiSI CCGG 2 cut(s) 35, 977
BslFI GGGAC 3 cut(s) 245, 959, 1103
BsmAI GTCTC 2 cut(s) 482, 1039
BsmFI GGGAC 3 cut(s) 245, 959, 1103
BsnI GGCC 3 cut(s) 358, 700, 1113
Bsp1286I GDGCHC 3 cut(s) 44, 641, 984
Bsp1407I TGTACA 1 cut(s) 816
Bsp143I GATC 4 cut(s) 162, 423, 554, 655
BspACI CCGC 1 cut(s) 788
BspANI GGCC 3 cut(s) 358, 700, 1113
BspCNI CTCAG 2 cut(s) 502, 1108
BspLI GGNNCC 5 cut(s) 33, 808, 930, 981, 1070
BspPI GGATC 1 cut(s) 418
BspQI GCTCTTC 2 cut(s) 471, 1063
BspT107I GGYRCC 3 cut(s) 806, 979, 1068
BsrFI RCCGGY 1 cut(s) 34
BsrGI TGTACA 1 cut(s) 816
BsrI ACTGG 1 cut(s) 33
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 3 cut(s) 43, 436, 760
BssMI GATC 4 cut(s) 162, 423, 554, 655
BssSI CACGAG 2 cut(s) 558, 869
BssT1I CCWWGG 2 cut(s) 436, 760
Bst2BI CACGAG 2 cut(s) 558, 869
Bst2UI CCWGG 1 cut(s) 45
Bst4CI ACNGT 2 cut(s) 65, 605
Bst6I CTCTTC 5 cut(s) 184, 218, 471, 507, 1063
BstAUI TGTACA 1 cut(s) 816
BstBAI YACGTR 1 cut(s) 253
BstC8I GCNNGC 1 cut(s) 356
BstDEI CTNAG 3 cut(s) 489, 579, 1116
BstF5I GGATG 1 cut(s) 208
BstH2I RGCGCY 1 cut(s) 465
BstHHI GCGC 1 cut(s) 464
BstKTI GATC 4 cut(s) 165, 426, 557, 658
BstMAI GTCTC 2 cut(s) 482, 1039
BstMBI GATC 4 cut(s) 162, 423, 554, 655
BstMWI GCNNNNNNNGC 1 cut(s) 858
BstNI CCWGG 1 cut(s) 45
BstNSI RCATGY 3 cut(s) 260, 823, 974
BstSCI CCNGG 2 cut(s) 43, 975
BstSLI GKGCMC 2 cut(s) 641, 984
BstV1I GCAGC 1 cut(s) 413
BstV2I GAAGAC 2 cut(s) 252, 933
BstX2I RGATCY 1 cut(s) 423
BstXI CCANNNNNNTGG 2 cut(s) 437, 1009
BstYI RGATCY 1 cut(s) 423
Bsu36I CCTNAGG 1 cut(s) 1116
BsuRI GGCC 3 cut(s) 358, 700, 1113
BtgZI GCGATG 1 cut(s) 456
BtrI CACGTC 1 cut(s) 711
BtsCI GGATG 1 cut(s) 208
BtsI GCAGTG 1 cut(s) 224
BtsIMutI CAGTG 1 cut(s) 224
Cac8I GCNNGC 1 cut(s) 356
CfoI GCGC 1 cut(s) 464
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 1 cut(s) 1112
Csp6I GTAC 4 cut(s) 66, 385, 817, 1069
CviAII CATG 8 cut(s) 187, 257, 398, 431, 457, 609, 820, 971
CviQI GTAC 4 cut(s) 66, 385, 817, 1069
DdeI CTNAG 3 cut(s) 489, 579, 1116
DpnI GATC 4 cut(s) 164, 425, 556, 657
DpnII GATC 4 cut(s) 162, 423, 554, 655
Eam1104I CTCTTC 5 cut(s) 184, 218, 471, 507, 1063
EarI CTCTTC 5 cut(s) 184, 218, 471, 507, 1063
Eco130I CCWWGG 2 cut(s) 436, 760
Eco24I GRGCYC 1 cut(s) 44
Eco57I CTGAAG 1 cut(s) 1044
Eco72I CACGTG 1 cut(s) 253
Eco81I CCTNAGG 1 cut(s) 1116
EcoO109I RGGNCCY 1 cut(s) 1112
EcoRII CCWGG 1 cut(s) 43
EcoT14I CCWWGG 2 cut(s) 436, 760
EcoT38I GRGCYC 1 cut(s) 44
ErhI CCWWGG 2 cut(s) 436, 760
FaeI CATG 8 cut(s) 190, 260, 401, 434, 460, 612, 823, 974
FalI AAGNNNNNCTT 2 cut(s) 925, 957
FaqI GGGAC 3 cut(s) 245, 959, 1103
FatI CATG 8 cut(s) 186, 256, 397, 430, 456, 608, 819, 970
FauI CCCGC 1 cut(s) 781
Fnu4HI GCNGC 1 cut(s) 402
FokI GGATG 1 cut(s) 215
FriOI GRGCYC 1 cut(s) 44
Fsp4HI GCNGC 1 cut(s) 402
FspBI CTAG 3 cut(s) 546, 803, 885
GlaI GCGC 1 cut(s) 463
GluI GCNGC 1 cut(s) 402
GsuI CTGGAG 1 cut(s) 50
HaeII RGCGCY 1 cut(s) 465
HaeIII GGCC 3 cut(s) 358, 700, 1113
HapII CCGG 2 cut(s) 35, 977
HhaI GCGC 1 cut(s) 464
Hin1II CATG 8 cut(s) 190, 260, 401, 434, 460, 612, 823, 974
Hin6I GCGC 1 cut(s) 462
HinP1I GCGC 1 cut(s) 462
HincII GTYRAC 1 cut(s) 714
HindII GTYRAC 1 cut(s) 714
HinfI GANTC 3 cut(s) 101, 290, 493
HpaII CCGG 2 cut(s) 35, 977
HphI GGTGA 2 cut(s) 49, 778
Hpy166II GTNNAC 2 cut(s) 708, 714
Hpy188I TCNGA 2 cut(s) 337, 492
Hpy188III TCNNGA 5 cut(s) 160, 276, 421, 751, 1042
Hpy8I GTNNAC 2 cut(s) 708, 714
HpyAV CCTTC 5 cut(s) 11, 188, 507, 528, 628
HpyCH4III ACNGT 2 cut(s) 65, 605
HpyCH4IV ACGT 2 cut(s) 252, 710
HpyCH4V TGCA 9 cut(s) 83, 256, 401, 533, 608, 823, 861, 913, 970
HpyF10VI GCNNNNNNNGC 1 cut(s) 858
HpyF3I CTNAG 3 cut(s) 489, 579, 1116
HpySE526I ACGT 2 cut(s) 252, 710
Hsp92II CATG 8 cut(s) 190, 260, 401, 434, 460, 612, 823, 974
HspAI GCGC 1 cut(s) 462
KpnI GGTACC 1 cut(s) 1072
Kzo9I GATC 4 cut(s) 162, 423, 554, 655
LguI GCTCTTC 2 cut(s) 471, 1063
LmnI GCTCC 2 cut(s) 31, 624
Lsp1109I GCAGC 1 cut(s) 413
LweI GCATC 2 cut(s) 341, 711
MaeI CTAG 3 cut(s) 546, 803, 885
MaeII ACGT 2 cut(s) 252, 710
MaeIII GTNAC 3 cut(s) 282, 766, 1120
MalI GATC 4 cut(s) 164, 425, 556, 657
MboI GATC 4 cut(s) 162, 423, 554, 655
MflI RGATCY 1 cut(s) 423
MhlI GDGCHC 3 cut(s) 44, 641, 984
MluCI AATT 8 cut(s) 53, 84, 114, 856, 898, 956, 1103, 1126
MmeI TCCRAC 3 cut(s) 222, 602, 664
MnlI CCTC 6 cut(s) 167, 219, 283, 727, 1103, 1111
MroXI GAANNNNTTC 1 cut(s) 1057
MseI TTAA 4 cut(s) 117, 144, 567, 1132
MslI CAYNNNNRTG 2 cut(s) 255, 812
MspA1I CMGCKG 1 cut(s) 404
MspI CCGG 2 cut(s) 35, 977
MspR9I CCNGG 2 cut(s) 45, 977
MvaI CCWGG 1 cut(s) 45
MwoI GCNNNNNNNGC 1 cut(s) 858
NciI CCSGG 1 cut(s) 977
NdeII GATC 4 cut(s) 162, 423, 554, 655
NlaIII CATG 8 cut(s) 190, 260, 401, 434, 460, 612, 823, 974
NlaIV GGNNCC 5 cut(s) 33, 808, 930, 981, 1070
NmuCI GTSAC 2 cut(s) 282, 766
NspI RCATGY 3 cut(s) 260, 823, 974
PciSI GCTCTTC 2 cut(s) 471, 1063
PdmI GAANNNNTTC 1 cut(s) 1057
PfeI GAWTC 3 cut(s) 101, 290, 493
PkrI GCNGC 1 cut(s) 403
PmaCI CACGTG 1 cut(s) 253
PmlI CACGTG 1 cut(s) 253
Ppu21I YACGTR 1 cut(s) 253
Psp6I CCWGG 1 cut(s) 43
PspCI CACGTG 1 cut(s) 253
PspGI CCWGG 1 cut(s) 43
PspN4I GGNNCC 5 cut(s) 33, 808, 930, 981, 1070
PspPI GGNCC 1 cut(s) 1112
PsuI RGATCY 1 cut(s) 423
PvuII CAGCTG 1 cut(s) 404
RsaI GTAC 4 cut(s) 67, 386, 818, 1070
RsaNI GTAC 4 cut(s) 66, 385, 817, 1069
RseI CAYNNNNRTG 2 cut(s) 255, 812
SapI GCTCTTC 2 cut(s) 471, 1063
SaqAI TTAA 4 cut(s) 117, 144, 567, 1132
SatI GCNGC 1 cut(s) 402
Sau3AI GATC 4 cut(s) 162, 423, 554, 655
Sau96I GGNCC 1 cut(s) 1112
ScaI AGTACT 1 cut(s) 67
ScrFI CCNGG 2 cut(s) 45, 977
SduI GDGCHC 3 cut(s) 44, 641, 984
SfaNI GCATC 2 cut(s) 341, 711
SmiMI CAYNNNNRTG 2 cut(s) 255, 812
SpeI ACTAGT 1 cut(s) 545
Sse9I AATT 8 cut(s) 53, 84, 114, 856, 898, 956, 1103, 1126
SsiI CCGC 1 cut(s) 788
SspI AATATT 1 cut(s) 417
SspMI CTAG 3 cut(s) 546, 803, 885
StyD4I CCNGG 2 cut(s) 43, 975
StyI CCWWGG 2 cut(s) 436, 760
TaaI ACNGT 2 cut(s) 65, 605
TaiI ACGT 2 cut(s) 255, 713
TaqI TCGA 3 cut(s) 553, 658, 989
TasI AATT 8 cut(s) 53, 84, 114, 856, 898, 956, 1103, 1126
TatI WGTACW 2 cut(s) 65, 816
TfiI GAWTC 3 cut(s) 101, 290, 493
Tru1I TTAA 4 cut(s) 117, 144, 567, 1132
Tru9I TTAA 4 cut(s) 117, 144, 567, 1132
TscAI CASTG 1 cut(s) 224
TseFI GTSAC 2 cut(s) 282, 766
TseI GCWGC 1 cut(s) 401
Tsp45I GTSAC 2 cut(s) 282, 766
TspDTI ATGAA 3 cut(s) 362, 555, 845
TspGWI ACGGA 1 cut(s) 82
TspRI CASTG 1 cut(s) 224
XapI RAATTY 1 cut(s) 1103
XceI RCATGY 3 cut(s) 260, 823, 974
XcmI CCANNNNNNNNNTGG 1 cut(s) 434
XmnI GAANNNNTTC 1 cut(s) 1057
XspI CTAG 3 cut(s) 546, 803, 885
ZrmI AGTACT 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.