RchiOBHm_Chr5g0050931

Wax ester synthase-like Acyl-CoA acyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000634
Physical Location & Seq
Reverse (-)
51761796 .. 51790739
28944 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32845

Sequence Viewer

Length: 1131 bp
ATGTCATCACCTGCATCATACGATATTTTCTTTGATGAGTATATAACGAAGATAGCATCAGAAACATTCCCACAAAGCAGGCCATTATGGGAACTTCATCTTTTCAAGTACCCAACAAGTCATGCAGCTGGACACATGATATTCAAGATCCACCATGCCCTTGGCGATGGCTACTCTCTCATGGGCGCTCTTCTCTCTTGCCTCCAGAATGCTCACAATCCTTCCATTCCTCTGACATTTCCGACATTAAAGAGTGCAAAAAATGAAACTAGTAGTCGATCTCGTGCGTTTGAGTTTATGCCGAAGATACTTTCTTCCGTTTTCAACGGTGCATGGGATTTCAGTTGGAGCATTTTGAAGGGCACTTGGGTTGAAGATGATCGAACACCAATAAGGTCCGGTGTTGAAGGAGTTGAGTTTCGACCTGTGTCGGTGTCAACCTTGATGCTGCCTATTGAGGAAATTAAACTTATCAAGAACAAGCTTGGAGTGACGATAAATGATGTGATTACAGGAACAATCTTTATGGGCATCCGAATGTACATTCAAGAGATGAATAGTGAAAAATCAAGTAGGCAAAATTGCACAGCACTGGTGTTGCTGAATACTAGACTAGTTGCGGGTTACAAGTCAGTGCAGGAGATGTTGATCGAAACAAATAAGTCTTGGGGAAACCGATTTGTGTTCTTGCAAGTCTCGGTGCCCAAGTCGAGTGAAGTTTCAAAGCCACTGGATTTTGTGTGGGAAGCACGTAATATAATCCAGAGACAGAGAAAATCTTCAGCTTGGTATCTCACCACTAGGCTCTGGGACATTTTGAAGAAATTTAGAGGCGCTGAGGCAGTATCTAAATATATCTATCGTACACTGAAAAACACAAGCATAGTAATCACAAATATGATTGGGCCATTGGAACAAATGAGTCTCGCCAATCACCCCATTGAAGGAATTTACTTCACAGTGACTGGTGCACCTCAGTGTCTTTCTGTAACAGTAATGAGTTATATGGGAAAGCTAAGGATTGCTTTGGTAGCTGAAAAAGACTTCATAGATACCAACGTATTGCAAGCATGCATGAAAGATGCATTTCGGGTGATAGGTGAAGCTGTGAATGAATTTCCTATAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1298 WES_acyltransf

Protein Analysis

376

Amino Acids

42.56

Weight (kDa)

9.18

Isoelectric Point (pI)

44.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WS_DGAT_cat PF03007 11 - 170 2.3e-10 Wax ester synthase/diacylglycerol acyltransferase catalytic domain
WS_DGAT_C PF06974 223 - 366 1.1e-37 WS/DGAT C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000634)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10290 FvH4_3g28230 FvH4_3g28240
malus_domestica MD05G1036400.v1.1 MD10G1159400.v1.1 MD17G1237100.v1.1 MD17G1237200.v1.1 MD17G1237300.v1.1 MD17G1237600.v1.1 MD17G1238200.v1.1
prunus_persica Prupe.3G140400_v2.0.a1 Prupe.3G140700_v2.0.a1 Prupe.3G141100_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G258100_v2.0.a1 Prupe.7G117300_v2.0.a1 Prupe.7G117400_v2.0.a1 Prupe.7G117600_v2.0.a1 Prupe.8G044700_v2.0.a1
pyrus_communis pycom05g02680 pycom17g24220 pycom17g24240
rosa_chinensis RchiOBHm_Chr1g0350141 RchiOBHm_Chr5g0050931 RchiOBHm_Chr5g0051001 RchiOBHm_Chr5g0051021 RchiOBHm_Chr6g0267491
rosa_laevigata RLG00000013999 RLG00000028502 RLG00000034744 RLG00000034748 RLG00000034753
rosa_multiflora Rmu_sc0000446.1_g000056 Rmu_sc0000782.1_g000006 Rmu_sc0002951.1_g000001 Rmu_sc0003674.1_g000026 Rmu_sc0003756.1_g000004 Rmu_sc0011917.1_g000004 Rmu_sc0012005.1_g000019 Rmu_sc0040739.1_g000001
rosa_roxburghii Rroxscaffold_1G00029730 Rroxscaffold_1G00029740 Rroxscaffold_4G00305060 Rroxscaffold_7G00200710
rosa_rugosa Rorug01G0208400 Rorug05G0265200.1 Rorug05G0265300.1 Rorug05G0265400.1 Rorug05G0265500.1 Rorug05G0294000 Rorug06G0033100 Rorug06G0243300
rosa_samantha Rh1BG192100 Rh1BG192200 Rh1DG220600 Rh5AG270300 Rh5AG337200 Rh5AG337700 Rh5BG346700 Rh5BG347400 Rh5CG374300 Rh5DG359700 Rh5DG360500 Rh6AG154500 Rh6BG155100 Rh6BG362300 Rh6CG150300 Rh6DG140300 Rh6DG356300
rosa_wichuraiana Rw0G018820 Rw1G019350 Rw5G025300 Rw5G031770 Rw5G031820 Rw5G031870 Rw5G031910 Rw6G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 19
Acc36I ACCTGC 1 cut(s) 19
AccB1I GGYRCC 1 cut(s) 700
AciI CCGC 1 cut(s) 620
AclWI GGATC 1 cut(s) 142
AcsI RAATTY 3 cut(s) 824, 948, 1115
AcuI CTGAAG 1 cut(s) 765
AfaI GTAC 3 cut(s) 110, 542, 865
AfiI CCNNNNNNNGG 1 cut(s) 944
AhlI ACTAGT 2 cut(s) 269, 613
AleI CACNNNNGTG 1 cut(s) 976
AluBI AGCT 6 cut(s) 128, 484, 785, 1015, 1034, 1106
AluI AGCT 6 cut(s) 128, 484, 785, 1015, 1034, 1106
Alw21I GWGCWC 1 cut(s) 973
Alw26I GTCTC 3 cut(s) 700, 760, 929
Alw44I GTGCAC 1 cut(s) 969
AlwI GGATC 1 cut(s) 142
AlwNI CAGNNNCTG 1 cut(s) 965
AoxI GGCC 2 cut(s) 80, 905
ApaLI GTGCAC 1 cut(s) 969
ApeKI GCWGC 2 cut(s) 125, 448
ApoI RAATTY 3 cut(s) 824, 948, 1115
Asp700I GAANNNNTTC 1 cut(s) 778
AspLEI GCGC 2 cut(s) 188, 836
AspS9I GGNCC 2 cut(s) 396, 905
AsuHPI GGTGA 4 cut(s) 787, 926, 1105, 1112
AvaII GGWCC 1 cut(s) 396
BaeGI GKGCMC 3 cut(s) 365, 705, 973
BaeI ACNNNNGTAYC 2 cut(s) 1044, 1077
BanI GGYRCC 1 cut(s) 700
BauI CACGAG 1 cut(s) 282
Bbv12I GWGCWC 1 cut(s) 973
BbvCI CCTCAGC 1 cut(s) 837
BbvI GCAGC 2 cut(s) 137, 435
BccI CCATC 1 cut(s) 161
BcoDI GTCTC 3 cut(s) 700, 760, 929
BcuI ACTAGT 2 cut(s) 269, 613
BfaI CTAG 4 cut(s) 270, 609, 614, 801
BfoI RGCGCY 2 cut(s) 189, 837
BfuAI ACCTGC 1 cut(s) 19
BisI GCNGC 2 cut(s) 126, 449
BlsI GCNGC 2 cut(s) 127, 450
Bme18I GGWCC 1 cut(s) 396
BmgT120I GGNCC 2 cut(s) 396, 905
BmiI GGNNCC 1 cut(s) 702
BmsI GCATC 5 cut(s) 23, 65, 435, 540, 1072
BoxI GACNNNNGTC 1 cut(s) 427
BpmI CTGGAG 1 cut(s) 188
Bpu10I CCTNAGC 2 cut(s) 837, 1016
BsaAI YACGTR 1 cut(s) 752
BsaBI GATNNNNATC 1 cut(s) 647
BsaJI CCNNGG 1 cut(s) 160
BsaWI WCCGGW 1 cut(s) 398
Bsc4I CCNNNNNNNGG 1 cut(s) 944
Bse1I ACTGG 3 cut(s) 597, 735, 970
Bse8I GATNNNNATC 1 cut(s) 647
BseDI CCNNGG 1 cut(s) 160
BseGI GGATG 1 cut(s) 531
BseJI GATNNNNATC 1 cut(s) 647
BseLI CCNNNNNNNGG 1 cut(s) 944
BseMII CTCAG 2 cut(s) 828, 989
BseNI ACTGG 3 cut(s) 597, 735, 970
BseSI GKGCMC 3 cut(s) 365, 705, 973
BseXI GCAGC 2 cut(s) 137, 435
BsgI GTGCAG 1 cut(s) 656
BshFI GGCC 2 cut(s) 82, 907
BshNI GGYRCC 1 cut(s) 700
BsiHKAI GWGCWC 1 cut(s) 973
BsiSI CCGG 1 cut(s) 399
BslFI GGGAC 1 cut(s) 824
BslI CCNNNNNNNGG 1 cut(s) 944
BsmAI GTCTC 3 cut(s) 700, 760, 929
BsmFI GGGAC 1 cut(s) 824
BsmI GAATGC 1 cut(s) 214
BsnI GGCC 2 cut(s) 82, 907
Bsp1286I GDGCHC 3 cut(s) 365, 705, 973
Bsp1407I TGTACA 1 cut(s) 540
Bsp143I GATC 4 cut(s) 147, 278, 379, 648
BspACI CCGC 1 cut(s) 620
BspANI GGCC 2 cut(s) 82, 907
BspCNI CTCAG 2 cut(s) 829, 988
BspLI GGNNCC 1 cut(s) 702
BspMI ACCTGC 1 cut(s) 19
BspPI GGATC 1 cut(s) 142
BspQI GCTCTTC 1 cut(s) 195
BspT107I GGYRCC 1 cut(s) 700
BsrGI TGTACA 1 cut(s) 540
BsrI ACTGG 3 cut(s) 597, 735, 970
BssECI CCNNGG 1 cut(s) 160
BssMI GATC 4 cut(s) 147, 278, 379, 648
BssSI CACGAG 1 cut(s) 282
BssT1I CCWWGG 1 cut(s) 160
Bst2BI CACGAG 1 cut(s) 282
Bst4CI ACNGT 3 cut(s) 329, 961, 994
Bst6I CTCTTC 1 cut(s) 195
BstAUI TGTACA 1 cut(s) 540
BstBAI YACGTR 1 cut(s) 752
BstC8I GCNNGC 3 cut(s) 80, 1068, 1072
BstDEI CTNAG 3 cut(s) 837, 975, 1016
BstF5I GGATG 1 cut(s) 531
BstH2I RGCGCY 2 cut(s) 189, 837
BstHHI GCGC 2 cut(s) 188, 836
BstKTI GATC 4 cut(s) 150, 281, 382, 651
BstMAI GTCTC 3 cut(s) 700, 760, 929
BstMBI GATC 4 cut(s) 147, 278, 379, 648
BstMWI GCNNNNNNNGC 1 cut(s) 1031
BstNSI RCATGY 1 cut(s) 1074
BstPAI GACNNNNGTC 1 cut(s) 427
BstSLI GKGCMC 3 cut(s) 365, 705, 973
BstV1I GCAGC 2 cut(s) 137, 435
BstX2I RGATCY 1 cut(s) 147
BstXI CCANNNNNNTGG 1 cut(s) 161
BstYI RGATCY 1 cut(s) 147
BsuRI GGCC 2 cut(s) 82, 907
BtgZI GCGATG 1 cut(s) 180
BtsCI GGATG 1 cut(s) 531
BtsIMutI CAGTG 6 cut(s) 590, 639, 728, 866, 966, 983
BveI ACCTGC 1 cut(s) 19
Cac8I GCNNGC 3 cut(s) 80, 1068, 1072
CaiI CAGNNNCTG 1 cut(s) 965
CfoI GCGC 2 cut(s) 188, 836
Cfr13I GGNCC 2 cut(s) 396, 905
Csp6I GTAC 3 cut(s) 109, 541, 864
CviAII CATG 7 cut(s) 122, 136, 155, 181, 333, 1071, 1075
CviQI GTAC 3 cut(s) 109, 541, 864
DdeI CTNAG 3 cut(s) 837, 975, 1016
DpnI GATC 4 cut(s) 149, 280, 381, 650
DpnII GATC 4 cut(s) 147, 278, 379, 648
Eam1104I CTCTTC 1 cut(s) 195
EarI CTCTTC 1 cut(s) 195
Eco130I CCWWGG 1 cut(s) 160
Eco47I GGWCC 1 cut(s) 396
Eco57I CTGAAG 1 cut(s) 765
EcoT14I CCWWGG 1 cut(s) 160
EcoT22I ATGCAT 2 cut(s) 1076, 1087
ErhI CCWWGG 1 cut(s) 160
FaeI CATG 7 cut(s) 125, 139, 158, 184, 336, 1074, 1078
FalI AAGNNNNNCTT 2 cut(s) 1009, 1041
FaqI GGGAC 1 cut(s) 824
FatI CATG 7 cut(s) 121, 135, 154, 180, 332, 1070, 1074
FauI CCCGC 1 cut(s) 613
Fnu4HI GCNGC 2 cut(s) 126, 449
FokI GGATG 1 cut(s) 518
Fsp4HI GCNGC 2 cut(s) 126, 449
FspBI CTAG 4 cut(s) 270, 609, 614, 801
GlaI GCGC 2 cut(s) 187, 835
GluI GCNGC 2 cut(s) 126, 449
GsuI CTGGAG 1 cut(s) 188
HaeII RGCGCY 2 cut(s) 189, 837
HaeIII GGCC 2 cut(s) 82, 907
HapII CCGG 1 cut(s) 399
HhaI GCGC 2 cut(s) 188, 836
Hin1II CATG 7 cut(s) 125, 139, 158, 184, 336, 1074, 1078
Hin6I GCGC 2 cut(s) 186, 834
HinP1I GCGC 2 cut(s) 186, 834
HincII GTYRAC 1 cut(s) 438
HindII GTYRAC 1 cut(s) 438
HindIII AAGCTT 1 cut(s) 482
HinfI GANTC 1 cut(s) 922
HpaII CCGG 1 cut(s) 399
HphI GGTGA 4 cut(s) 787, 926, 1105, 1112
Hpy166II GTNNAC 3 cut(s) 438, 866, 971
Hpy188I TCNGA 4 cut(s) 61, 234, 243, 536
Hpy188III TCNNGA 5 cut(s) 145, 205, 475, 548, 763
Hpy8I GTNNAC 3 cut(s) 438, 866, 971
HpyAV CCTTC 4 cut(s) 231, 352, 401, 938
HpyCH4III ACNGT 3 cut(s) 329, 961, 994
HpyCH4IV ACGT 2 cut(s) 751, 1059
HpyF10VI GCNNNNNNNGC 1 cut(s) 1031
HpyF3I CTNAG 3 cut(s) 837, 975, 1016
HpySE526I ACGT 2 cut(s) 751, 1059
Hsp92II CATG 7 cut(s) 125, 139, 158, 184, 336, 1074, 1078
HspAI GCGC 2 cut(s) 186, 834
Kzo9I GATC 4 cut(s) 147, 278, 379, 648
LguI GCTCTTC 1 cut(s) 195
LmnI GCTCC 1 cut(s) 348
Lsp1109I GCAGC 2 cut(s) 137, 435
LweI GCATC 5 cut(s) 23, 65, 435, 540, 1072
MaeI CTAG 4 cut(s) 270, 609, 614, 801
MaeII ACGT 2 cut(s) 751, 1059
MaeIII GTNAC 4 cut(s) 490, 623, 961, 988
MalI GATC 4 cut(s) 149, 280, 381, 650
MboI GATC 4 cut(s) 147, 278, 379, 648
MboII GAAGA 7 cut(s) 61, 182, 306, 316, 386, 771, 832
MflI RGATCY 1 cut(s) 147
MhlI GDGCHC 3 cut(s) 365, 705, 973
MluCI AATT 5 cut(s) 462, 580, 824, 948, 1115
MlyI GAGTC 1 cut(s) 931
MmeI TCCRAC 2 cut(s) 266, 326
MnlI CCTC 6 cut(s) 212, 240, 451, 824, 832, 984
Mph1103I ATGCAT 2 cut(s) 1076, 1087
MroXI GAANNNNTTC 1 cut(s) 778
MseI TTAA 2 cut(s) 248, 465
MslI CAYNNNNRTG 3 cut(s) 536, 896, 976
MspA1I CMGCKG 1 cut(s) 128
MspI CCGG 1 cut(s) 399
Mva1269I GAATGC 1 cut(s) 214
MwoI GCNNNNNNNGC 1 cut(s) 1031
NdeII GATC 4 cut(s) 147, 278, 379, 648
NlaIII CATG 7 cut(s) 125, 139, 158, 184, 336, 1074, 1078
NlaIV GGNNCC 1 cut(s) 702
NmuCI GTSAC 2 cut(s) 490, 961
NsiI ATGCAT 2 cut(s) 1076, 1087
NspI RCATGY 1 cut(s) 1074
OliI CACNNNNGTG 1 cut(s) 976
PaeI GCATGC 1 cut(s) 1074
PaqCI CACCTGC 1 cut(s) 19
PciSI GCTCTTC 1 cut(s) 195
PctI GAATGC 1 cut(s) 214
PdmI GAANNNNTTC 1 cut(s) 778
PkrI GCNGC 2 cut(s) 127, 450
PleI GAGTC 1 cut(s) 930
PpsI GAGTC 1 cut(s) 930
Ppu21I YACGTR 1 cut(s) 752
PshAI GACNNNNGTC 1 cut(s) 427
PspN4I GGNNCC 1 cut(s) 702
PspPI GGNCC 2 cut(s) 396, 905
PstNI CAGNNNCTG 1 cut(s) 965
PsuI RGATCY 1 cut(s) 147
PvuII CAGCTG 1 cut(s) 128
RsaI GTAC 3 cut(s) 110, 542, 865
RsaNI GTAC 3 cut(s) 109, 541, 864
RseI CAYNNNNRTG 3 cut(s) 536, 896, 976
SapI GCTCTTC 1 cut(s) 195
SaqAI TTAA 2 cut(s) 248, 465
SatI GCNGC 2 cut(s) 126, 449
Sau3AI GATC 4 cut(s) 147, 278, 379, 648
Sau96I GGNCC 2 cut(s) 396, 905
SchI GAGTC 1 cut(s) 931
SduI GDGCHC 3 cut(s) 365, 705, 973
SfaNI GCATC 5 cut(s) 23, 65, 435, 540, 1072
SinI GGWCC 1 cut(s) 396
SmiMI CAYNNNNRTG 3 cut(s) 536, 896, 976
SpeI ACTAGT 2 cut(s) 269, 613
SphI GCATGC 1 cut(s) 1074
Sse9I AATT 5 cut(s) 462, 580, 824, 948, 1115
SsiI CCGC 1 cut(s) 620
SspMI CTAG 4 cut(s) 270, 609, 614, 801
StyI CCWWGG 1 cut(s) 160
TaaI ACNGT 3 cut(s) 329, 961, 994
TaiI ACGT 2 cut(s) 754, 1062
TaqI TCGA 5 cut(s) 277, 382, 421, 651, 710
TasI AATT 5 cut(s) 462, 580, 824, 948, 1115
TatI WGTACW 1 cut(s) 540
Tru1I TTAA 2 cut(s) 248, 465
Tru9I TTAA 2 cut(s) 248, 465
TscAI CASTG 6 cut(s) 597, 639, 735, 873, 966, 983
TseFI GTSAC 2 cut(s) 490, 961
TseI GCWGC 2 cut(s) 125, 448
Tsp45I GTSAC 2 cut(s) 490, 961
TspDTI ATGAA 6 cut(s) 86, 279, 569, 1036, 1091, 1128
TspGWI ACGGA 1 cut(s) 307
TspRI CASTG 6 cut(s) 597, 639, 735, 873, 966, 983
VneI GTGCAC 1 cut(s) 969
VpaK11BI GGWCC 1 cut(s) 396
XapI RAATTY 3 cut(s) 824, 948, 1115
XceI RCATGY 1 cut(s) 1074
XcmI CCANNNNNNNNNTGG 1 cut(s) 158
XmnI GAANNNNTTC 1 cut(s) 778
XspI CTAG 4 cut(s) 270, 609, 614, 801
Zsp2I ATGCAT 2 cut(s) 1076, 1087
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.