MD17G1238200.v1.1

O-acyltransferase (WSD1-like)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000634
Physical Location & Seq
Forward (+)
28745671 .. 28748593
2923 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1238200.v1.1.491

Sequence Viewer

Length: 849 bp
ATGACCAAAACTTTCTCATCCTTGTTGAACACCATATCCGATATTTGGTGGGGCATTTCAAAGAGCATCACTGGGGAAGATGATCTATCACCAATAAAATCCTCGAACAATGAACTTGAGTTCACGCCAATTACAGTATCAACTGTGACGATCTCTCTTGATCACATCAGATTAATTAAGAACAGGCTTGAAGTGACGATAAATGACGTCCTTACCGGGATGGTCTTTCTTGGCTCTCGACTATATATGCAAGAGATGAACCAAAGCTCAAGGGAAGCCCATAGCACTGCATTGGTTTTACTAAATACGAGGATCATGGGGAATTATACTTCGATTGAAGAAATGGTCAAACCCGACAGCAAGAGCCCATGGGGAAATCATTTTGCATTCTTGCAGGTTCCCATTCCCAAGTTGACTGAATTCTCAAATGCGCTTAACTTCGTCTGGGAGGCTCAAAAATTTATCAAGATGAAAAGAAATTCTTTAGCTATTTATTTCACTAGTGGGGTATTGAAGATGTTGAACATGTTTGAAGGCCATGAGGCAGCATCAAGATACATCCGTAGAACATTAAAGAACTCGAGCATGGCATTCACAAATATGATTGGGCCCGTGGAACAAATGTCTTTGGCAAACCAACCAATAAAAGGATTATACTTTATGTTAGTCGGATCACCTCAGGATCTTGACATATCAGTCCTAAGTTATATGGGAAAGGTGAGGCTTACCTTAACAATGAAAAAAGGCACCATAGATCCACAAAAACTGAAGTTGTGCATGGAAAACGCCCATGATATGATATTCAATGCCTCGGACAAATATCCTGTGGAAAATAGCACTGAAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1298 WES_acyltransf

Protein Analysis

283

Amino Acids

31.94

Weight (kDa)

8.81

Isoelectric Point (pI)

34.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WS_DGAT_C PF06974 124 - 267 1.7e-36 WS/DGAT C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000634)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10290 FvH4_3g28230 FvH4_3g28240
malus_domestica MD05G1036400.v1.1 MD10G1159400.v1.1 MD17G1237100.v1.1 MD17G1237200.v1.1 MD17G1237300.v1.1 MD17G1237600.v1.1 MD17G1238200.v1.1
prunus_persica Prupe.3G140400_v2.0.a1 Prupe.3G140700_v2.0.a1 Prupe.3G141100_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G258100_v2.0.a1 Prupe.7G117300_v2.0.a1 Prupe.7G117400_v2.0.a1 Prupe.7G117600_v2.0.a1 Prupe.8G044700_v2.0.a1
pyrus_communis pycom05g02680 pycom17g24220 pycom17g24240
rosa_chinensis RchiOBHm_Chr1g0350141 RchiOBHm_Chr5g0050931 RchiOBHm_Chr5g0051001 RchiOBHm_Chr5g0051021 RchiOBHm_Chr6g0267491
rosa_laevigata RLG00000013999 RLG00000028502 RLG00000034744 RLG00000034748 RLG00000034753
rosa_multiflora Rmu_sc0000446.1_g000056 Rmu_sc0000782.1_g000006 Rmu_sc0002951.1_g000001 Rmu_sc0003674.1_g000026 Rmu_sc0003756.1_g000004 Rmu_sc0011917.1_g000004 Rmu_sc0012005.1_g000019 Rmu_sc0040739.1_g000001
rosa_roxburghii Rroxscaffold_1G00029730 Rroxscaffold_1G00029740 Rroxscaffold_4G00305060 Rroxscaffold_7G00200710
rosa_rugosa Rorug01G0208400 Rorug05G0265200.1 Rorug05G0265300.1 Rorug05G0265400.1 Rorug05G0265500.1 Rorug05G0294000 Rorug06G0033100 Rorug06G0243300
rosa_samantha Rh1BG192100 Rh1BG192200 Rh1DG220600 Rh5AG270300 Rh5AG337200 Rh5AG337700 Rh5BG346700 Rh5BG347400 Rh5CG374300 Rh5DG359700 Rh5DG360500 Rh6AG154500 Rh6BG155100 Rh6BG362300 Rh6CG150300 Rh6DG140300 Rh6DG356300
rosa_wichuraiana Rw0G018820 Rw1G019350 Rw5G025300 Rw5G031770 Rw5G031820 Rw5G031870 Rw5G031910 Rw6G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 695
AatII GACGTC 1 cut(s) 210
Acc36I ACCTGC 1 cut(s) 385
AccB1I GGYRCC 1 cut(s) 746
AclWI GGATC 4 cut(s) 320, 679, 690, 749
AcsI RAATTY 3 cut(s) 419, 458, 478
AcuI CTGAAG 1 cut(s) 788
AcyI GRCGYC 1 cut(s) 207
AfiI CCNNNNNNNGG 2 cut(s) 45, 647
AflIII ACRYGT 1 cut(s) 525
AgsI TTSAA 8 cut(s) 28, 60, 191, 338, 514, 523, 533, 805
AhlI ACTAGT 1 cut(s) 500
AloI GAACNNNNNNTCC 2 cut(s) 20, 52
AluBI AGCT 2 cut(s) 267, 488
AluI AGCT 2 cut(s) 267, 488
AlwI GGATC 4 cut(s) 320, 679, 690, 749
Ama87I CYCGRG 1 cut(s) 580
AoxI GGCC 2 cut(s) 535, 608
ApaI GGGCCC 1 cut(s) 612
ApeKI GCWGC 1 cut(s) 545
ApoI RAATTY 3 cut(s) 419, 458, 478
AseI ATTAAT 1 cut(s) 173
AspLEI GCGC 1 cut(s) 433
AspS9I GGNCC 2 cut(s) 608, 609
AsuC2I CCSGG 1 cut(s) 217
AsuHPI GGTGA 3 cut(s) 81, 666, 730
AvaI CYCGRG 1 cut(s) 580
AxyI CCTNAGG 1 cut(s) 678
BaeGI GKGCMC 1 cut(s) 612
BaeI ACNNNNGTAYC 2 cut(s) 547, 580
BanI GGYRCC 1 cut(s) 746
BanII GRGCYC 2 cut(s) 368, 612
BbvI GCAGC 1 cut(s) 557
BccI CCATC 1 cut(s) 214
BclI TGATCA 1 cut(s) 160
BcnI CCSGG 1 cut(s) 217
BcuI ACTAGT 1 cut(s) 500
BfaI CTAG 1 cut(s) 501
BfuAI ACCTGC 1 cut(s) 385
BisI GCNGC 1 cut(s) 546
BlsI GCNGC 1 cut(s) 547
Bme1390I CCNGG 1 cut(s) 217
BmeT110I CYCGRG 1 cut(s) 580
BmgT120I GGNCC 2 cut(s) 608, 609
BmiI GGNNCC 3 cut(s) 399, 610, 748
BmrFI CCNGG 1 cut(s) 217
BmrI ACTGGG 1 cut(s) 81
BmsI GCATC 2 cut(s) 75, 557
BmuI ACTGGG 1 cut(s) 81
BpuEI CTTGAG 2 cut(s) 137, 253
BpuMI CCSGG 1 cut(s) 217
BsaHI GRCGYC 1 cut(s) 207
BsaJI CCNNGG 3 cut(s) 368, 612, 810
Bsc4I CCNNNNNNNGG 2 cut(s) 45, 647
Bse1I ACTGG 1 cut(s) 76
Bse21I CCTNAGG 1 cut(s) 678
BseDI CCNNGG 3 cut(s) 368, 612, 810
BseGI GGATG 3 cut(s) 17, 225, 558
BseLI CCNNNNNNNGG 2 cut(s) 45, 647
BseMII CTCAG 1 cut(s) 692
BseNI ACTGG 1 cut(s) 76
BseSI GKGCMC 1 cut(s) 612
BseXI GCAGC 1 cut(s) 557
BshFI GGCC 2 cut(s) 537, 610
BshNI GGYRCC 1 cut(s) 746
BsiHKCI CYCGRG 1 cut(s) 580
BsiSI CCGG 1 cut(s) 216
BslI CCNNNNNNNGG 2 cut(s) 45, 647
BsmI GAATGC 2 cut(s) 386, 590
BsnI GGCC 2 cut(s) 537, 610
BsoBI CYCGRG 1 cut(s) 580
Bsp120I GGGCCC 1 cut(s) 608
Bsp1286I GDGCHC 2 cut(s) 368, 612
Bsp143I GATC 7 cut(s) 82, 150, 160, 312, 671, 682, 754
Bsp19I CCATGG 1 cut(s) 368
BspANI GGCC 2 cut(s) 537, 610
BspCNI CTCAG 1 cut(s) 691
BspLI GGNNCC 3 cut(s) 399, 610, 748
BspMI ACCTGC 1 cut(s) 385
BspPI GGATC 4 cut(s) 320, 679, 690, 749
BspT107I GGYRCC 1 cut(s) 746
BsrI ACTGG 1 cut(s) 76
BssECI CCNNGG 3 cut(s) 368, 612, 810
BssMI GATC 7 cut(s) 82, 150, 160, 312, 671, 682, 754
BssNI GRCGYC 1 cut(s) 207
BssT1I CCWWGG 1 cut(s) 368
Bst4CI ACNGT 2 cut(s) 136, 145
BstACI GRCGYC 1 cut(s) 207
BstDEI CTNAG 2 cut(s) 678, 701
BstDSI CCRYGG 2 cut(s) 368, 612
BstF5I GGATG 3 cut(s) 17, 225, 558
BstHHI GCGC 1 cut(s) 433
BstKTI GATC 7 cut(s) 85, 153, 163, 315, 674, 685, 757
BstMBI GATC 7 cut(s) 82, 150, 160, 312, 671, 682, 754
BstNSI RCATGY 1 cut(s) 529
BstSCI CCNGG 1 cut(s) 215
BstSLI GKGCMC 1 cut(s) 612
BstV1I GCAGC 1 cut(s) 557
BstX2I RGATCY 2 cut(s) 682, 754
BstYI RGATCY 2 cut(s) 682, 754
Bsu36I CCTNAGG 1 cut(s) 678
BsuRI GGCC 2 cut(s) 537, 610
BtgI CCRYGG 2 cut(s) 368, 612
BtsCI GGATG 3 cut(s) 17, 225, 558
BtsI GCAGTG 1 cut(s) 285
BtsIMutI CAGTG 3 cut(s) 69, 285, 837
BveI ACCTGC 1 cut(s) 385
CfoI GCGC 1 cut(s) 433
Cfr13I GGNCC 2 cut(s) 608, 609
CviAII CATG 7 cut(s) 316, 369, 526, 539, 586, 778, 791
DdeI CTNAG 2 cut(s) 678, 701
DpnI GATC 7 cut(s) 84, 152, 162, 314, 673, 684, 756
DpnII GATC 7 cut(s) 82, 150, 160, 312, 671, 682, 754
DrdI GACNNNNNNGTC 1 cut(s) 695
DseDI GACNNNNNNGTC 1 cut(s) 695
Eco130I CCWWGG 1 cut(s) 368
Eco24I GRGCYC 2 cut(s) 368, 612
Eco57I CTGAAG 1 cut(s) 788
Eco81I CCTNAGG 1 cut(s) 678
Eco88I CYCGRG 1 cut(s) 580
EcoRI GAATTC 1 cut(s) 419
EcoT14I CCWWGG 1 cut(s) 368
EcoT38I GRGCYC 2 cut(s) 368, 612
ErhI CCWWGG 1 cut(s) 368
FaeI CATG 7 cut(s) 319, 372, 529, 542, 589, 781, 794
FalI AAGNNNNNCTT 2 cut(s) 466, 498
FatI CATG 7 cut(s) 315, 368, 525, 538, 585, 777, 790
FbaI TGATCA 1 cut(s) 160
Fnu4HI GCNGC 1 cut(s) 546
FokI GGATG 3 cut(s) 4, 232, 545
FriOI GRGCYC 2 cut(s) 368, 612
Fsp4HI GCNGC 1 cut(s) 546
FspBI CTAG 1 cut(s) 501
GlaI GCGC 1 cut(s) 432
GluI GCNGC 1 cut(s) 546
HaeIII GGCC 2 cut(s) 537, 610
HapII CCGG 1 cut(s) 216
HhaI GCGC 1 cut(s) 433
Hin1I GRCGYC 1 cut(s) 207
Hin1II CATG 7 cut(s) 319, 372, 529, 542, 589, 781, 794
Hin6I GCGC 1 cut(s) 431
HinP1I GCGC 1 cut(s) 431
HincII GTYRAC 1 cut(s) 414
HindII GTYRAC 1 cut(s) 414
HpaII CCGG 1 cut(s) 216
HphI GGTGA 3 cut(s) 81, 666, 730
Hpy166II GTNNAC 2 cut(s) 123, 414
Hpy188I TCNGA 4 cut(s) 40, 170, 671, 814
Hpy188III TCNNGA 6 cut(s) 158, 237, 466, 552, 680, 686
Hpy8I GTNNAC 2 cut(s) 123, 414
HpyAV CCTTC 1 cut(s) 527
HpyCH4III ACNGT 2 cut(s) 136, 145
HpyCH4IV ACGT 1 cut(s) 207
HpyCH4V TGCA 5 cut(s) 250, 290, 386, 394, 777
HpyF3I CTNAG 2 cut(s) 678, 701
HpySE526I ACGT 1 cut(s) 207
Hsp92I GRCGYC 1 cut(s) 207
Hsp92II CATG 7 cut(s) 319, 372, 529, 542, 589, 781, 794
HspAI GCGC 1 cut(s) 431
Ksp22I TGATCA 1 cut(s) 160
Kzo9I GATC 7 cut(s) 82, 150, 160, 312, 671, 682, 754
LpnPI CCDG 7 cut(s) 57, 169, 229, 380, 430, 665, 837
Lsp1109I GCAGC 1 cut(s) 557
LweI GCATC 2 cut(s) 75, 557
MaeI CTAG 1 cut(s) 501
MaeII ACGT 1 cut(s) 207
MaeIII GTNAC 2 cut(s) 145, 193
MalI GATC 7 cut(s) 84, 152, 162, 314, 673, 684, 756
MboI GATC 7 cut(s) 82, 150, 160, 312, 671, 682, 754
MboII GAAGA 3 cut(s) 89, 350, 526
MflI RGATCY 2 cut(s) 682, 754
MhlI GDGCHC 2 cut(s) 368, 612
MluCI AATT 6 cut(s) 129, 174, 322, 419, 458, 478
MmeI TCCRAC 1 cut(s) 649
MnlI CCTC 7 cut(s) 112, 303, 442, 535, 687, 714, 820
MseI TTAA 5 cut(s) 173, 177, 435, 572, 731
MslI CAYNNNNRTG 1 cut(s) 599
MspI CCGG 1 cut(s) 216
MspR9I CCNGG 1 cut(s) 217
Mva1269I GAATGC 2 cut(s) 386, 590
NciI CCSGG 1 cut(s) 217
NcoI CCATGG 1 cut(s) 368
NdeII GATC 7 cut(s) 82, 150, 160, 312, 671, 682, 754
NlaIII CATG 7 cut(s) 319, 372, 529, 542, 589, 781, 794
NlaIV GGNNCC 3 cut(s) 399, 610, 748
NmuCI GTSAC 2 cut(s) 145, 193
NspI RCATGY 1 cut(s) 529
PacI TTAATTAA 1 cut(s) 177
PaeR7I CTCGAG 1 cut(s) 580
PciI ACATGT 1 cut(s) 525
PctI GAATGC 2 cut(s) 386, 590
PkrI GCNGC 1 cut(s) 547
PscI ACATGT 1 cut(s) 525
PshBI ATTAAT 1 cut(s) 173
PspN4I GGNNCC 3 cut(s) 399, 610, 748
PspOMI GGGCCC 1 cut(s) 608
PspPI GGNCC 2 cut(s) 608, 609
PspXI VCTCGAGB 1 cut(s) 580
PsuI RGATCY 2 cut(s) 682, 754
RseI CAYNNNNRTG 1 cut(s) 599
SaqAI TTAA 5 cut(s) 173, 177, 435, 572, 731
SatI GCNGC 1 cut(s) 546
Sau3AI GATC 7 cut(s) 82, 150, 160, 312, 671, 682, 754
Sau96I GGNCC 2 cut(s) 608, 609
ScrFI CCNGG 1 cut(s) 217
SduI GDGCHC 2 cut(s) 368, 612
SetI ASST 7 cut(s) 210, 269, 399, 490, 679, 720, 731
SfaNI GCATC 2 cut(s) 75, 557
Sfr274I CTCGAG 1 cut(s) 580
SlaI CTCGAG 1 cut(s) 580
SmiMI CAYNNNNRTG 1 cut(s) 599
SmlI CTYRAG 3 cut(s) 116, 268, 580
SmoI CTYRAG 3 cut(s) 116, 268, 580
SpeI ACTAGT 1 cut(s) 500
Sse9I AATT 6 cut(s) 129, 174, 322, 419, 458, 478
SspMI CTAG 1 cut(s) 501
StyD4I CCNGG 1 cut(s) 215
StyI CCWWGG 1 cut(s) 368
TaaI ACNGT 2 cut(s) 136, 145
TaiI ACGT 1 cut(s) 210
TaqI TCGA 4 cut(s) 104, 238, 332, 581
TasI AATT 6 cut(s) 129, 174, 322, 419, 458, 478
Tru1I TTAA 5 cut(s) 173, 177, 435, 572, 731
Tru9I TTAA 5 cut(s) 173, 177, 435, 572, 731
TscAI CASTG 3 cut(s) 76, 292, 844
TseFI GTSAC 2 cut(s) 145, 193
TseI GCWGC 1 cut(s) 545
Tsp45I GTSAC 2 cut(s) 145, 193
TspDTI ATGAA 4 cut(s) 126, 272, 485, 752
TspGWI ACGGA 1 cut(s) 551
TspRI CASTG 3 cut(s) 76, 292, 844
VspI ATTAAT 1 cut(s) 173
XapI RAATTY 3 cut(s) 419, 458, 478
XceI RCATGY 1 cut(s) 529
XhoI CTCGAG 1 cut(s) 580
XspI CTAG 1 cut(s) 501
ZraI GACGTC 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.