RchiOBHm_Chr1g0350141

Wax ester synthase-like Acyl-CoA acyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000634
Physical Location & Seq
Forward (+)
43216582 .. 43237844
21263 bp
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UTR
Exon/CDS
Intron
PRQ57602

Sequence Viewer

Length: 1401 bp
ATGGAGTTTGGAGAAGAAGGACTACTACTGGAGCCGGTGAGCCCTGGTGGTCAGTTCTTCAACAGTACTGTCTTATCTCTTGCAATAATTTCCGTTTTGGAATTGGAATCACAATTTTCAATGAACGATTCTCAAACTTTTTCACTTCTTAAAACTGTGTTCCTCCCGATCAATCCACGCTTCTCTTCCATCATGGTTGAAGGTGTGGATGAAAAGAATAAGCAGTGGAAGAGGGTTCAAGTGAAGTTGGAAGACCATGTGCATGTCCCCATTTTCCCCTCTGGAATGTCACTTGAATCATACGATACTTACTTTGATGAGTATATAACGAAGATAGCATCAGAAACATTCCCACAAAGCAGGCCATTGTGGGAACTTCATATTTTCAAGTACCCAACAAGTCATGCAGCTGGACAAATAATATTCAAGATCCACCATGCCCTTGGCGATGGCTACTCTCTCATGGGCGCTCTTCTCTCTTGTCTCCAAAATGCTCACAATCCTTCTCTTCCCCTGACATTTCCTTCACTAAAGGGTGCAAAGAATGAAACTAGTAGTCGATCTCGTGTATTTGAGTTTGTACCTAAGATATTTTCTGCTGTTATCAACGGTGCATGGGATTTCGGTTGGAGCATTTTGAAGGGCACTTGGGTTGAAGATGATCGAACACCAATAAGGTCAGGCGTTGTTGGAGTTGAGTTTCGGCCTTTGTCCACGTCAACCTTGATGCTGTCTATTGAGGAAATCAAACTTATCAAGAACAAGCTTGGGGTGACGATAAATGATGTTATTGCGGGAATAATCTTTCTGGGCACCCGAATGTACATGCAACAGATGAATAGTGAAAAATCAAGTAGCCAGAATTGCACGGCACTCGTGTTGCTGAATACTAGACTTGTTGCGAATTACAAGTCGGTGCAGGAGATGTTGATGGAACCAAGCAAGTCTTCTTGGGGCAACCAATTTGTGTTCTTGCATGTCCCGGTGCCCAAGTCGAGTGAAGTTTCCAAGCCATTGGATTTTGTATGGGAAGCACATAAAATAGTCAAGAGACATAGAAGCTCTTCAGCTTGGTACCTTACTATTAGGCTCTGGGACATTTTGAAGAAATTTAGAGGCCCTGAGGCAGTAGCTGGATACATTCATAACACACTAATGAACTCAAGCATGACAATTACAAATATGATCGGGCCACTGGAACAAATGGCTTTGGCTGGTAACCCTATTAAAGGAATCTACTTTATGGTGGTCGGTTCACCTCAGAGTCTTACTATATCAGCAATAAGTTATATGGGAAAGCTAAGGATTGCCATAGGAGCTGAAAAAGGTCTCGTAGATTCCAACAAATTGCAGGCATGCATGAAAGATGCATTTCGGGTGATATCTGAAGCTGCGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1298 WES_acyltransf

Protein Analysis

466

Amino Acids

52.07

Weight (kDa)

8.53

Isoelectric Point (pI)

46.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WS_DGAT_cat PF03007 67 - 264 2.1e-09 Wax ester synthase/diacylglycerol acyltransferase catalytic domain
WS_DGAT_C PF06974 318 - 461 9.2e-37 WS/DGAT C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000634)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10290 FvH4_3g28230 FvH4_3g28240
malus_domestica MD05G1036400.v1.1 MD10G1159400.v1.1 MD17G1237100.v1.1 MD17G1237200.v1.1 MD17G1237300.v1.1 MD17G1237600.v1.1 MD17G1238200.v1.1
prunus_persica Prupe.3G140400_v2.0.a1 Prupe.3G140700_v2.0.a1 Prupe.3G141100_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G258100_v2.0.a1 Prupe.7G117300_v2.0.a1 Prupe.7G117400_v2.0.a1 Prupe.7G117600_v2.0.a1 Prupe.8G044700_v2.0.a1
pyrus_communis pycom05g02680 pycom17g24220 pycom17g24240
rosa_chinensis RchiOBHm_Chr1g0350141 RchiOBHm_Chr5g0050931 RchiOBHm_Chr5g0051001 RchiOBHm_Chr5g0051021 RchiOBHm_Chr6g0267491
rosa_laevigata RLG00000013999 RLG00000028502 RLG00000034744 RLG00000034748 RLG00000034753
rosa_multiflora Rmu_sc0000446.1_g000056 Rmu_sc0000782.1_g000006 Rmu_sc0002951.1_g000001 Rmu_sc0003674.1_g000026 Rmu_sc0003756.1_g000004 Rmu_sc0011917.1_g000004 Rmu_sc0012005.1_g000019 Rmu_sc0040739.1_g000001
rosa_roxburghii Rroxscaffold_1G00029730 Rroxscaffold_1G00029740 Rroxscaffold_4G00305060 Rroxscaffold_7G00200710
rosa_rugosa Rorug01G0208400 Rorug05G0265200.1 Rorug05G0265300.1 Rorug05G0265400.1 Rorug05G0265500.1 Rorug05G0294000 Rorug06G0033100 Rorug06G0243300
rosa_samantha Rh1BG192100 Rh1BG192200 Rh1DG220600 Rh5AG270300 Rh5AG337200 Rh5AG337700 Rh5BG346700 Rh5BG347400 Rh5CG374300 Rh5DG359700 Rh5DG360500 Rh6AG154500 Rh6BG155100 Rh6BG362300 Rh6CG150300 Rh6DG140300 Rh6DG356300
rosa_wichuraiana Rw0G018820 Rw1G019350 Rw5G025300 Rw5G031770 Rw5G031820 Rw5G031870 Rw5G031910 Rw6G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1074
AccB1I GGYRCC 3 cut(s) 812, 985, 1074
AciI CCGC 1 cut(s) 794
AclWI GGATC 1 cut(s) 424
AcsI RAATTY 1 cut(s) 1109
AcuI CTGAAG 1 cut(s) 1050
AfaI GTAC 5 cut(s) 67, 392, 582, 824, 1076
AfiI CCNNNNNNNGG 1 cut(s) 1229
AhlI ACTAGT 1 cut(s) 551
AjiI CACGTC 1 cut(s) 717
AjnI CCWGG 1 cut(s) 43
AjuI GAANNNNNNNTTGG 2 cut(s) 931, 963
AluBI AGCT 8 cut(s) 410, 766, 1062, 1070, 1133, 1300, 1319, 1391
AluI AGCT 8 cut(s) 410, 766, 1062, 1070, 1133, 1300, 1319, 1391
Alw26I GTCTC 3 cut(s) 488, 1045, 1334
AlwI GGATC 1 cut(s) 424
AlwNI CAGNNNCTG 1 cut(s) 1133
AoxI GGCC 4 cut(s) 362, 704, 1117, 1190
ApeKI GCWGC 2 cut(s) 407, 1391
ApoI RAATTY 1 cut(s) 1109
ArsI GACNNNNNNTTYG 2 cut(s) 896, 928
Asp700I GAANNNNTTC 1 cut(s) 1063
Asp718I GGTACC 1 cut(s) 1074
AspLEI GCGC 1 cut(s) 470
AspS9I GGNCC 2 cut(s) 1118, 1190
AsuC2I CCSGG 1 cut(s) 983
AsuHPI GGTGA 4 cut(s) 49, 784, 1248, 1390
AxyI CCTNAGG 1 cut(s) 1122
BaeGI GKGCMC 3 cut(s) 647, 815, 990
BanI GGYRCC 3 cut(s) 812, 985, 1074
BanII GRGCYC 1 cut(s) 44
BarI GAAGNNNNNNTAC 3 cut(s) 9, 38, 41
BauI CACGAG 2 cut(s) 564, 875
BbsI GAAGAC 2 cut(s) 258, 939
BbvI GCAGC 2 cut(s) 419, 1378
BccI CCATC 3 cut(s) 197, 443, 925
BceAI ACGGC 1 cut(s) 885
BcgI CGANNNNNNTGC 2 cut(s) 856, 890
BciT130I CCWGG 1 cut(s) 45
BciVI GTATCC 1 cut(s) 1130
BcnI CCSGG 1 cut(s) 983
BcoDI GTCTC 3 cut(s) 488, 1045, 1334
BcuI ACTAGT 1 cut(s) 551
BfaI CTAG 2 cut(s) 552, 891
BfoI RGCGCY 1 cut(s) 471
BfuI GTATCC 1 cut(s) 1130
BisI GCNGC 2 cut(s) 408, 1392
BlsI GCNGC 2 cut(s) 409, 1393
BmcAI AGTACT 1 cut(s) 67
Bme1390I CCNGG 2 cut(s) 45, 983
BmgBI CACGTC 1 cut(s) 717
BmgT120I GGNCC 2 cut(s) 1118, 1190
BmiI GGNNCC 5 cut(s) 33, 814, 936, 987, 1076
BmrFI CCNGG 2 cut(s) 45, 983
BmsI GCATC 3 cut(s) 347, 717, 1357
BpiI GAAGAC 2 cut(s) 258, 939
BpmI CTGGAG 1 cut(s) 50
Bpu10I CCTNAGC 1 cut(s) 1301
BpuEI CTTGAG 1 cut(s) 1147
BpuMI CCSGG 1 cut(s) 983
BsaI GGTCTC 1 cut(s) 1334
BsaJI CCNNGG 2 cut(s) 43, 442
Bsc4I CCNNNNNNNGG 1 cut(s) 1229
Bse118I RCCGGY 1 cut(s) 34
Bse1I ACTGG 2 cut(s) 33, 1200
Bse21I CCTNAGG 1 cut(s) 1122
BseBI CCWGG 1 cut(s) 45
BseDI CCNNGG 2 cut(s) 43, 442
BseGI GGATG 1 cut(s) 214
BseLI CCNNNNNNNGG 1 cut(s) 1229
BseMII CTCAG 2 cut(s) 1113, 1274
BseNI ACTGG 2 cut(s) 33, 1200
BseSI GKGCMC 3 cut(s) 647, 815, 990
BseXI GCAGC 2 cut(s) 419, 1378
BsgI GTGCAG 1 cut(s) 938
BshFI GGCC 4 cut(s) 364, 706, 1119, 1192
BshNI GGYRCC 3 cut(s) 812, 985, 1074
BsiSI CCGG 2 cut(s) 35, 983
BslFI GGGAC 3 cut(s) 251, 965, 1109
BslI CCNNNNNNNGG 1 cut(s) 1229
BsmAI GTCTC 3 cut(s) 488, 1045, 1334
BsmFI GGGAC 3 cut(s) 251, 965, 1109
BsnI GGCC 4 cut(s) 364, 706, 1119, 1192
Bso31I GGTCTC 1 cut(s) 1334
Bsp1286I GDGCHC 4 cut(s) 44, 647, 815, 990
Bsp1407I TGTACA 1 cut(s) 822
Bsp143I GATC 5 cut(s) 168, 429, 560, 661, 1185
BspACI CCGC 1 cut(s) 794
BspANI GGCC 4 cut(s) 364, 706, 1119, 1192
BspCNI CTCAG 2 cut(s) 1114, 1273
BspLI GGNNCC 5 cut(s) 33, 814, 936, 987, 1076
BspPI GGATC 1 cut(s) 424
BspQI GCTCTTC 2 cut(s) 477, 1069
BspT107I GGYRCC 3 cut(s) 812, 985, 1074
BspTNI GGTCTC 1 cut(s) 1334
BsrFI RCCGGY 1 cut(s) 34
BsrGI TGTACA 1 cut(s) 822
BsrI ACTGG 2 cut(s) 33, 1200
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 2 cut(s) 43, 442
BssMI GATC 5 cut(s) 168, 429, 560, 661, 1185
BssSI CACGAG 2 cut(s) 564, 875
BssT1I CCWWGG 1 cut(s) 442
Bst2BI CACGAG 2 cut(s) 564, 875
Bst2UI CCWGG 1 cut(s) 45
Bst4CI ACNGT 4 cut(s) 65, 70, 157, 611
Bst6I CTCTTC 5 cut(s) 190, 224, 477, 513, 1069
BstAUI TGTACA 1 cut(s) 822
BstC8I GCNNGC 3 cut(s) 362, 1353, 1357
BstDEI CTNAG 4 cut(s) 585, 1122, 1260, 1301
BstEII GGTNACC 1 cut(s) 1217
BstENI CCTNNNNNAGG 1 cut(s) 1227
BstF5I GGATG 1 cut(s) 214
BstH2I RGCGCY 1 cut(s) 471
BstHHI GCGC 1 cut(s) 470
BstKTI GATC 5 cut(s) 171, 432, 563, 664, 1188
BstMAI GTCTC 3 cut(s) 488, 1045, 1334
BstMBI GATC 5 cut(s) 168, 429, 560, 661, 1185
BstMWI GCNNNNNNNGC 2 cut(s) 864, 1316
BstNI CCWGG 1 cut(s) 45
BstNSI RCATGY 4 cut(s) 266, 829, 980, 1359
BstPI GGTNACC 1 cut(s) 1217
BstSCI CCNGG 2 cut(s) 43, 981
BstSLI GKGCMC 3 cut(s) 647, 815, 990
BstV1I GCAGC 2 cut(s) 419, 1378
BstV2I GAAGAC 2 cut(s) 258, 939
BstX2I RGATCY 1 cut(s) 429
BstXI CCANNNNNNTGG 2 cut(s) 443, 1015
BstYI RGATCY 1 cut(s) 429
Bsu36I CCTNAGG 1 cut(s) 1122
BsuI GTATCC 1 cut(s) 1130
BsuRI GGCC 4 cut(s) 364, 706, 1119, 1192
BtgZI GCGATG 1 cut(s) 462
BtrI CACGTC 1 cut(s) 717
BtsCI GGATG 1 cut(s) 214
BtsI GCAGTG 1 cut(s) 230
BtsIMutI CAGTG 2 cut(s) 230, 1193
Cac8I GCNNGC 3 cut(s) 362, 1353, 1357
CaiI CAGNNNCTG 1 cut(s) 1133
CfoI GCGC 1 cut(s) 470
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 2 cut(s) 1118, 1190
Csp6I GTAC 5 cut(s) 66, 391, 581, 823, 1075
CviQI GTAC 5 cut(s) 66, 391, 581, 823, 1075
DdeI CTNAG 4 cut(s) 585, 1122, 1260, 1301
DpnI GATC 5 cut(s) 170, 431, 562, 663, 1187
DpnII GATC 5 cut(s) 168, 429, 560, 661, 1185
Eam1104I CTCTTC 5 cut(s) 190, 224, 477, 513, 1069
EarI CTCTTC 5 cut(s) 190, 224, 477, 513, 1069
Eco130I CCWWGG 1 cut(s) 442
Eco24I GRGCYC 1 cut(s) 44
Eco31I GGTCTC 1 cut(s) 1334
Eco32I GATATC 1 cut(s) 1383
Eco57I CTGAAG 1 cut(s) 1050
Eco81I CCTNAGG 1 cut(s) 1122
Eco91I GGTNACC 1 cut(s) 1217
EcoNI CCTNNNNNAGG 1 cut(s) 1227
EcoO109I RGGNCCY 1 cut(s) 1118
EcoO65I GGTNACC 1 cut(s) 1217
EcoRII CCWGG 1 cut(s) 43
EcoRV GATATC 1 cut(s) 1383
EcoT14I CCWWGG 1 cut(s) 442
EcoT22I ATGCAT 2 cut(s) 1361, 1372
EcoT38I GRGCYC 1 cut(s) 44
ErhI CCWWGG 1 cut(s) 442
FalI AAGNNNNNCTT 2 cut(s) 931, 963
FaqI GGGAC 3 cut(s) 251, 965, 1109
FauI CCCGC 1 cut(s) 787
Fnu4HI GCNGC 2 cut(s) 408, 1392
FokI GGATG 1 cut(s) 221
FriOI GRGCYC 1 cut(s) 44
Fsp4HI GCNGC 2 cut(s) 408, 1392
FspBI CTAG 2 cut(s) 552, 891
GlaI GCGC 1 cut(s) 469
GluI GCNGC 2 cut(s) 408, 1392
GsuI CTGGAG 1 cut(s) 50
HaeII RGCGCY 1 cut(s) 471
HaeIII GGCC 4 cut(s) 364, 706, 1119, 1192
HapII CCGG 2 cut(s) 35, 983
HhaI GCGC 1 cut(s) 470
Hin6I GCGC 1 cut(s) 468
HinP1I GCGC 1 cut(s) 468
HincII GTYRAC 1 cut(s) 720
HindII GTYRAC 1 cut(s) 720
HindIII AAGCTT 1 cut(s) 764
HinfI GANTC 6 cut(s) 107, 128, 296, 1233, 1264, 1337
HpaII CCGG 2 cut(s) 35, 983
HphI GGTGA 4 cut(s) 49, 784, 1248, 1390
Hpy166II GTNNAC 3 cut(s) 714, 720, 1256
Hpy188I TCNGA 3 cut(s) 343, 1263, 1387
Hpy188III TCNNGA 5 cut(s) 166, 282, 427, 757, 1048
Hpy8I GTNNAC 3 cut(s) 714, 720, 1256
HpyAV CCTTC 5 cut(s) 11, 194, 513, 534, 634
HpyCH4III ACNGT 4 cut(s) 65, 70, 157, 611
HpyCH4IV ACGT 1 cut(s) 716
HpyF10VI GCNNNNNNNGC 2 cut(s) 864, 1316
HpyF3I CTNAG 4 cut(s) 585, 1122, 1260, 1301
HpySE526I ACGT 1 cut(s) 716
HspAI GCGC 1 cut(s) 468
KpnI GGTACC 1 cut(s) 1078
Kzo9I GATC 5 cut(s) 168, 429, 560, 661, 1185
LguI GCTCTTC 2 cut(s) 477, 1069
LmnI GCTCC 3 cut(s) 31, 630, 1316
Lsp1109I GCAGC 2 cut(s) 419, 1378
LweI GCATC 3 cut(s) 347, 717, 1357
MaeI CTAG 2 cut(s) 552, 891
MaeII ACGT 1 cut(s) 716
MaeIII GTNAC 3 cut(s) 288, 772, 1217
MalI GATC 5 cut(s) 170, 431, 562, 663, 1187
MboI GATC 5 cut(s) 168, 429, 560, 661, 1185
MflI RGATCY 1 cut(s) 429
MhlI GDGCHC 4 cut(s) 44, 647, 815, 990
MlyI GAGTC 1 cut(s) 1273
MmeI TCCRAC 4 cut(s) 228, 608, 670, 1365
MnlI CCTC 7 cut(s) 173, 225, 289, 733, 1109, 1117, 1269
Mph1103I ATGCAT 2 cut(s) 1361, 1372
MroXI GAANNNNTTC 1 cut(s) 1063
MseI TTAA 3 cut(s) 150, 1227, 1399
MslI CAYNNNNRTG 3 cut(s) 261, 818, 1154
MspA1I CMGCKG 1 cut(s) 410
MspI CCGG 2 cut(s) 35, 983
MspR9I CCNGG 2 cut(s) 45, 983
MvaI CCWGG 1 cut(s) 45
MwoI GCNNNNNNNGC 2 cut(s) 864, 1316
NciI CCSGG 1 cut(s) 983
NdeII GATC 5 cut(s) 168, 429, 560, 661, 1185
NlaIV GGNNCC 5 cut(s) 33, 814, 936, 987, 1076
NmuCI GTSAC 2 cut(s) 288, 772
NsiI ATGCAT 2 cut(s) 1361, 1372
NspI RCATGY 4 cut(s) 266, 829, 980, 1359
PaeI GCATGC 1 cut(s) 1359
PciSI GCTCTTC 2 cut(s) 477, 1069
PdmI GAANNNNTTC 1 cut(s) 1063
PfeI GAWTC 5 cut(s) 107, 128, 296, 1233, 1337
PkrI GCNGC 2 cut(s) 409, 1393
PleI GAGTC 1 cut(s) 1272
PpsI GAGTC 1 cut(s) 1272
Psp6I CCWGG 1 cut(s) 43
PspEI GGTNACC 1 cut(s) 1217
PspGI CCWGG 1 cut(s) 43
PspN4I GGNNCC 5 cut(s) 33, 814, 936, 987, 1076
PspPI GGNCC 2 cut(s) 1118, 1190
PstNI CAGNNNCTG 1 cut(s) 1133
PsuI RGATCY 1 cut(s) 429
PvuII CAGCTG 1 cut(s) 410
RsaI GTAC 5 cut(s) 67, 392, 582, 824, 1076
RsaNI GTAC 5 cut(s) 66, 391, 581, 823, 1075
RseI CAYNNNNRTG 3 cut(s) 261, 818, 1154
SapI GCTCTTC 2 cut(s) 477, 1069
SaqAI TTAA 3 cut(s) 150, 1227, 1399
SatI GCNGC 2 cut(s) 408, 1392
Sau3AI GATC 5 cut(s) 168, 429, 560, 661, 1185
Sau96I GGNCC 2 cut(s) 1118, 1190
ScaI AGTACT 1 cut(s) 67
SchI GAGTC 1 cut(s) 1273
ScrFI CCNGG 2 cut(s) 45, 983
SduI GDGCHC 4 cut(s) 44, 647, 815, 990
SfaNI GCATC 3 cut(s) 347, 717, 1357
SmiMI CAYNNNNRTG 3 cut(s) 261, 818, 1154
SmlI CTYRAG 1 cut(s) 1162
SmoI CTYRAG 1 cut(s) 1162
SpeI ACTAGT 1 cut(s) 551
SphI GCATGC 1 cut(s) 1359
SsiI CCGC 1 cut(s) 794
SspI AATATT 1 cut(s) 423
SspMI CTAG 2 cut(s) 552, 891
StyD4I CCNGG 2 cut(s) 43, 981
StyI CCWWGG 1 cut(s) 442
TaaI ACNGT 4 cut(s) 65, 70, 157, 611
TaiI ACGT 1 cut(s) 719
TaqI TCGA 3 cut(s) 559, 664, 995
TatI WGTACW 2 cut(s) 65, 822
TfiI GAWTC 5 cut(s) 107, 128, 296, 1233, 1337
Tru1I TTAA 3 cut(s) 150, 1227, 1399
Tru9I TTAA 3 cut(s) 150, 1227, 1399
TscAI CASTG 2 cut(s) 230, 1200
TseFI GTSAC 2 cut(s) 288, 772
TseI GCWGC 2 cut(s) 407, 1391
Tsp45I GTSAC 2 cut(s) 288, 772
TspDTI ATGAA 8 cut(s) 137, 225, 368, 561, 851, 1133, 1172, 1376
TspGWI ACGGA 1 cut(s) 82
TspRI CASTG 2 cut(s) 230, 1200
XagI CCTNNNNNAGG 1 cut(s) 1227
XapI RAATTY 1 cut(s) 1109
XceI RCATGY 4 cut(s) 266, 829, 980, 1359
XcmI CCANNNNNNNNNTGG 1 cut(s) 440
XmnI GAANNNNTTC 1 cut(s) 1063
XspI CTAG 2 cut(s) 552, 891
ZrmI AGTACT 1 cut(s) 67
Zsp2I ATGCAT 2 cut(s) 1361, 1372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.