Rroxscaffold_4G00305060

O-acyltransferase (WSD1-like)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000634
Physical Location & Seq
Reverse (-)
25718134 .. 25737948
19815 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00305060.1

Sequence Viewer

Length: 1476 bp
ATGTTCCAAGGAAGACCGGAAATATATTATACAGAGACGGCAAAACATAGCCCGGCGCGGCTAGTATCAAATAATATGGAGTTTGGAGAAGAAGGACTACTACTGGAGCCGGTGAGCCCTGCTGGTCAATTCCTCAACAGTTCTGTCTTATCTCTTGCAATTATTTCCGTTTTGGAATTGGAATCACAATTTTCAATTAACGATTCTCAAACTTTTTCACTTCTTAAAAATGTGTTCCTCCCGATCAATCCACGCTTCTCTTCCATCATGGTTGAAGGTGTGGATGAAAAGAATAAGCAGTGGAAGAGGGTTCAAGTGAAGTTGGAAGACCATGTGCATGTCCCCATTTTCCCCTCTGGAATGTCACTTGAATCATACGATACTTACTTTGATGAGTATATAACGAAGATAGCATCAGAAACATTCCCACAAAGCAGGCCATTGTGGGAACTTCATATTTTCAAGTACCCAACAAGTCATGCAGCTGGACAAATAATATTCAAGATCCACCATGCCCTTGGCGATGGCTACTCTCTCATGGGCGCTCTTCTCTCTTGTCTCCAAAATGCACACAATCCTTCTCTTCCCCTGACATTTCCTTCACTAAAGGGTGCAAAGAATGAAACTAGTAGTCGATCTCGTGTATTTGAGTTTGTACCTAAGATATTTTCTGCTGTTATCAACGGTGCATGGGATTTCGGTTGGAGCATTTTGAAGGGCACTTGGGTTGAAGATGATCGAACACCAATACGGTCAGGCGTTGTTGGAGTTGAGTTTCGGCCTGTGTCCATGTCAACCTTGATGCTGTCTATTGAGGAAATCAAACTTATCAAGAACAAGCTTGGGGTGACGATAAATGATGTTATTGCGGGAACAATCTTTCTAGGCACCCGAATGTACATGCAACAGATGAATAGTGAAAAATCAAGTAGCCAGAATTGCACGGCACTCGTGTTGCTGAATACTAGACTTGTTGCGAATTACAAGTCGGTGCAGGAGATGTTGATGGAACCAAGCAAGTCTTCTTGGGGCAACCAATTTGTGTTCTTGCATGTCCCGGTGCCCAAGTCGAGTGAAGTTTCCAAGCCATTGGATTTTGTATGGGAAGCACAGAAGATAGTCAAAAGACATAGAAGCTCTTCAGCTTGGTACCTTACTATTAGGCTCTGGGACATGTTGAAGAGATTTAGAGGCCCTGAGACAGTAGCTGGATACATTCATAGCACACTAATGAACTCAAGCATGACAATCACAAATATGATCGGGCCACTGGAACAAATGGCTGTGGCTGATCACCCCATTAAAGGAATCTACTTTATGGTGGTCGGTTCACCTCAGAGTCTTACTATAACAGCAATGAGTTATATGGGAAAGCTAAGGATTGCCATAGGAGCTGAAAAAGGTTTCATAGATTCCAACAAATTGCAGGCATGCATGGAAGATGCATTTCGGGTGATATCTGAGGCTGCGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1298 WES_acyltransf

Protein Analysis

491

Amino Acids

55.12

Weight (kDa)

7.74

Isoelectric Point (pI)

50.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WS_DGAT_cat PF03007 94 - 289 3.4e-09 Wax ester synthase/diacylglycerol acyltransferase catalytic domain
WS_DGAT_C PF06974 343 - 486 1e-36 WS/DGAT C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000634)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10290 FvH4_3g28230 FvH4_3g28240
malus_domestica MD05G1036400.v1.1 MD10G1159400.v1.1 MD17G1237100.v1.1 MD17G1237200.v1.1 MD17G1237300.v1.1 MD17G1237600.v1.1 MD17G1238200.v1.1
prunus_persica Prupe.3G140400_v2.0.a1 Prupe.3G140700_v2.0.a1 Prupe.3G141100_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G258100_v2.0.a1 Prupe.7G117300_v2.0.a1 Prupe.7G117400_v2.0.a1 Prupe.7G117600_v2.0.a1 Prupe.8G044700_v2.0.a1
pyrus_communis pycom05g02680 pycom17g24220 pycom17g24240
rosa_chinensis RchiOBHm_Chr1g0350141 RchiOBHm_Chr5g0050931 RchiOBHm_Chr5g0051001 RchiOBHm_Chr5g0051021 RchiOBHm_Chr6g0267491
rosa_laevigata RLG00000013999 RLG00000028502 RLG00000034744 RLG00000034748 RLG00000034753
rosa_multiflora Rmu_sc0000446.1_g000056 Rmu_sc0000782.1_g000006 Rmu_sc0002951.1_g000001 Rmu_sc0003674.1_g000026 Rmu_sc0003756.1_g000004 Rmu_sc0011917.1_g000004 Rmu_sc0012005.1_g000019 Rmu_sc0040739.1_g000001
rosa_roxburghii Rroxscaffold_1G00029730 Rroxscaffold_1G00029740 Rroxscaffold_4G00305060 Rroxscaffold_7G00200710
rosa_rugosa Rorug01G0208400 Rorug05G0265200.1 Rorug05G0265300.1 Rorug05G0265400.1 Rorug05G0265500.1 Rorug05G0294000 Rorug06G0033100 Rorug06G0243300
rosa_samantha Rh1BG192100 Rh1BG192200 Rh1DG220600 Rh5AG270300 Rh5AG337200 Rh5AG337700 Rh5BG346700 Rh5BG347400 Rh5CG374300 Rh5DG359700 Rh5DG360500 Rh6AG154500 Rh6BG155100 Rh6BG362300 Rh6CG150300 Rh6DG140300 Rh6DG356300
rosa_wichuraiana Rw0G018820 Rw1G019350 Rw5G025300 Rw5G031770 Rw5G031820 Rw5G031870 Rw5G031910 Rw6G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1149
AccB1I GGYRCC 3 cut(s) 887, 1060, 1149
AccII CGCG 1 cut(s) 58
AciI CCGC 2 cut(s) 58, 869
AclWI GGATC 1 cut(s) 499
AcuI CTGAAG 1 cut(s) 1125
AfaI GTAC 4 cut(s) 467, 657, 899, 1151
AfiI CCNNNNNNNGG 1 cut(s) 1304
AflIII ACRYGT 1 cut(s) 1173
AgsI TTSAA 9 cut(s) 195, 275, 314, 371, 463, 502, 715, 731, 1180
AhlI ACTAGT 1 cut(s) 626
AjuI GAANNNNNNNTTGG 2 cut(s) 1006, 1038
AluBI AGCT 7 cut(s) 485, 841, 1137, 1145, 1208, 1375, 1394
AluI AGCT 7 cut(s) 485, 841, 1137, 1145, 1208, 1375, 1394
Alw26I GTCTC 3 cut(s) 29, 563, 1193
AlwI GGATC 1 cut(s) 499
AlwNI CAGNNNCTG 1 cut(s) 1208
AoxI GGCC 4 cut(s) 437, 779, 1192, 1265
ApeKI GCWGC 2 cut(s) 482, 1466
ArsI GACNNNNNNTTYG 2 cut(s) 971, 1003
Asp700I GAANNNNTTC 1 cut(s) 1138
Asp718I GGTACC 1 cut(s) 1149
AspLEI GCGC 2 cut(s) 58, 545
AspS9I GGNCC 2 cut(s) 1193, 1265
AsuC2I CCSGG 2 cut(s) 53, 1058
AsuHPI GGTGA 5 cut(s) 124, 859, 1286, 1323, 1465
BaeGI GKGCMC 2 cut(s) 722, 1065
BanI GGYRCC 3 cut(s) 887, 1060, 1149
BanII GRGCYC 1 cut(s) 119
BarI GAAGNNNNNNTAC 4 cut(s) 81, 84, 113, 116
BauI CACGAG 2 cut(s) 639, 950
BbsI GAAGAC 3 cut(s) 19, 333, 1014
BbvI GCAGC 2 cut(s) 494, 1453
BccI CCATC 3 cut(s) 272, 518, 1000
BceAI ACGGC 2 cut(s) 54, 960
BcgI CGANNNNNNTGC 2 cut(s) 931, 965
BciVI GTATCC 1 cut(s) 1205
BclI TGATCA 1 cut(s) 1291
BcnI CCSGG 2 cut(s) 53, 1058
BcoDI GTCTC 3 cut(s) 29, 563, 1193
BcuI ACTAGT 1 cut(s) 626
BfaI CTAG 4 cut(s) 62, 627, 884, 966
BfoI RGCGCY 1 cut(s) 546
BfuI GTATCC 1 cut(s) 1205
BisI GCNGC 3 cut(s) 59, 483, 1467
BlsI GCNGC 3 cut(s) 60, 484, 1468
Bme1390I CCNGG 2 cut(s) 53, 1058
BmgT120I GGNCC 2 cut(s) 1193, 1265
BmiI GGNNCC 5 cut(s) 108, 889, 1011, 1062, 1151
BmrFI CCNGG 2 cut(s) 53, 1058
BmsI GCATC 3 cut(s) 422, 792, 1432
BpiI GAAGAC 3 cut(s) 19, 333, 1014
BpmI CTGGAG 1 cut(s) 125
Bpu10I CCTNAGC 1 cut(s) 1376
BpuEI CTTGAG 1 cut(s) 1222
BpuMI CCSGG 2 cut(s) 53, 1058
BsaJI CCNNGG 2 cut(s) 7, 517
BsaWI WCCGGW 1 cut(s) 16
Bsc4I CCNNNNNNNGG 1 cut(s) 1304
Bse118I RCCGGY 1 cut(s) 109
Bse1I ACTGG 2 cut(s) 108, 1275
Bse3DI GCAATG 1 cut(s) 1362
BseDI CCNNGG 2 cut(s) 7, 517
BseGI GGATG 1 cut(s) 289
BseLI CCNNNNNNNGG 1 cut(s) 1304
BseMI GCAATG 1 cut(s) 1362
BseMII CTCAG 3 cut(s) 1188, 1349, 1452
BseNI ACTGG 2 cut(s) 108, 1275
BseSI GKGCMC 2 cut(s) 722, 1065
BseXI GCAGC 2 cut(s) 494, 1453
BsgI GTGCAG 1 cut(s) 1013
Bsh1236I CGCG 1 cut(s) 58
BshFI GGCC 4 cut(s) 439, 781, 1194, 1267
BshNI GGYRCC 3 cut(s) 887, 1060, 1149
BsiSI CCGG 4 cut(s) 17, 53, 110, 1058
BslFI GGGAC 3 cut(s) 326, 1040, 1184
BslI CCNNNNNNNGG 1 cut(s) 1304
BsmAI GTCTC 3 cut(s) 29, 563, 1193
BsmBI CGTCTC 1 cut(s) 29
BsmFI GGGAC 3 cut(s) 326, 1040, 1184
BsnI GGCC 4 cut(s) 439, 781, 1194, 1267
Bsp1286I GDGCHC 3 cut(s) 119, 722, 1065
Bsp1407I TGTACA 1 cut(s) 897
Bsp143I GATC 6 cut(s) 243, 504, 635, 736, 1260, 1291
BspACI CCGC 2 cut(s) 58, 869
BspANI GGCC 4 cut(s) 439, 781, 1194, 1267
BspCNI CTCAG 3 cut(s) 1189, 1348, 1453
BspFNI CGCG 1 cut(s) 58
BspLI GGNNCC 5 cut(s) 108, 889, 1011, 1062, 1151
BspPI GGATC 1 cut(s) 499
BspQI GCTCTTC 2 cut(s) 552, 1144
BspT107I GGYRCC 3 cut(s) 887, 1060, 1149
BsrDI GCAATG 1 cut(s) 1362
BsrFI RCCGGY 1 cut(s) 109
BsrGI TGTACA 1 cut(s) 897
BsrI ACTGG 2 cut(s) 108, 1275
BssAI RCCGGY 1 cut(s) 109
BssECI CCNNGG 2 cut(s) 7, 517
BssMI GATC 6 cut(s) 243, 504, 635, 736, 1260, 1291
BssSI CACGAG 2 cut(s) 639, 950
BssT1I CCWWGG 2 cut(s) 7, 517
Bst2BI CACGAG 2 cut(s) 639, 950
Bst4CI ACNGT 4 cut(s) 140, 686, 753, 1204
Bst6I CTCTTC 6 cut(s) 265, 299, 552, 588, 1144, 1175
BstAUI TGTACA 1 cut(s) 897
BstC8I GCNNGC 3 cut(s) 437, 1428, 1432
BstDEI CTNAG 5 cut(s) 660, 1197, 1335, 1376, 1461
BstF5I GGATG 1 cut(s) 289
BstFNI CGCG 1 cut(s) 58
BstH2I RGCGCY 1 cut(s) 546
BstHHI GCGC 2 cut(s) 58, 545
BstKTI GATC 6 cut(s) 246, 507, 638, 739, 1263, 1294
BstMAI GTCTC 3 cut(s) 29, 563, 1193
BstMBI GATC 6 cut(s) 243, 504, 635, 736, 1260, 1291
BstMWI GCNNNNNNNGC 2 cut(s) 939, 1391
BstNSI RCATGY 5 cut(s) 341, 904, 1055, 1177, 1434
BstSCI CCNGG 2 cut(s) 51, 1056
BstSLI GKGCMC 2 cut(s) 722, 1065
BstUI CGCG 1 cut(s) 58
BstV1I GCAGC 2 cut(s) 494, 1453
BstV2I GAAGAC 3 cut(s) 19, 333, 1014
BstX2I RGATCY 1 cut(s) 504
BstXI CCANNNNNNTGG 2 cut(s) 518, 1090
BstYI RGATCY 1 cut(s) 504
BsuI GTATCC 1 cut(s) 1205
BsuRI GGCC 4 cut(s) 439, 781, 1194, 1267
BtgZI GCGATG 1 cut(s) 537
BtsCI GGATG 1 cut(s) 289
BtsI GCAGTG 1 cut(s) 305
BtsIMutI CAGTG 2 cut(s) 305, 1268
Cac8I GCNNGC 3 cut(s) 437, 1428, 1432
CaiI CAGNNNCTG 1 cut(s) 1208
CfoI GCGC 2 cut(s) 58, 545
Cfr10I RCCGGY 1 cut(s) 109
Cfr13I GGNCC 2 cut(s) 1193, 1265
Csp6I GTAC 4 cut(s) 466, 656, 898, 1150
CviQI GTAC 4 cut(s) 466, 656, 898, 1150
DdeI CTNAG 5 cut(s) 660, 1197, 1335, 1376, 1461
DpnI GATC 6 cut(s) 245, 506, 637, 738, 1262, 1293
DpnII GATC 6 cut(s) 243, 504, 635, 736, 1260, 1291
Eam1104I CTCTTC 6 cut(s) 265, 299, 552, 588, 1144, 1175
EarI CTCTTC 6 cut(s) 265, 299, 552, 588, 1144, 1175
Eco130I CCWWGG 2 cut(s) 7, 517
Eco24I GRGCYC 1 cut(s) 119
Eco32I GATATC 1 cut(s) 1458
Eco57I CTGAAG 1 cut(s) 1125
EcoO109I RGGNCCY 1 cut(s) 1193
EcoRV GATATC 1 cut(s) 1458
EcoT14I CCWWGG 2 cut(s) 7, 517
EcoT22I ATGCAT 2 cut(s) 1436, 1447
EcoT38I GRGCYC 1 cut(s) 119
ErhI CCWWGG 2 cut(s) 7, 517
Esp3I CGTCTC 1 cut(s) 29
FalI AAGNNNNNCTT 2 cut(s) 1006, 1038
FaqI GGGAC 3 cut(s) 326, 1040, 1184
FauI CCCGC 1 cut(s) 862
FbaI TGATCA 1 cut(s) 1291
Fnu4HI GCNGC 3 cut(s) 59, 483, 1467
FokI GGATG 1 cut(s) 296
FriOI GRGCYC 1 cut(s) 119
Fsp4HI GCNGC 3 cut(s) 59, 483, 1467
FspBI CTAG 4 cut(s) 62, 627, 884, 966
GlaI GCGC 2 cut(s) 57, 544
GluI GCNGC 3 cut(s) 59, 483, 1467
GsuI CTGGAG 1 cut(s) 125
HaeII RGCGCY 1 cut(s) 546
HaeIII GGCC 4 cut(s) 439, 781, 1194, 1267
HapII CCGG 4 cut(s) 17, 53, 110, 1058
HhaI GCGC 2 cut(s) 58, 545
Hin6I GCGC 2 cut(s) 56, 543
HinP1I GCGC 2 cut(s) 56, 543
HincII GTYRAC 1 cut(s) 795
HindII GTYRAC 1 cut(s) 795
HindIII AAGCTT 1 cut(s) 839
HinfI GANTC 6 cut(s) 182, 203, 371, 1308, 1339, 1412
HpaII CCGG 4 cut(s) 17, 53, 110, 1058
HphI GGTGA 5 cut(s) 124, 859, 1286, 1323, 1465
Hpy166II GTNNAC 2 cut(s) 795, 1331
Hpy188I TCNGA 3 cut(s) 418, 1338, 1462
Hpy188III TCNNGA 4 cut(s) 241, 357, 502, 832
Hpy8I GTNNAC 2 cut(s) 795, 1331
HpyAV CCTTC 5 cut(s) 86, 269, 588, 609, 709
HpyCH4III ACNGT 4 cut(s) 140, 686, 753, 1204
HpyF10VI GCNNNNNNNGC 2 cut(s) 939, 1391
HpyF3I CTNAG 5 cut(s) 660, 1197, 1335, 1376, 1461
HspAI GCGC 2 cut(s) 56, 543
KpnI GGTACC 1 cut(s) 1153
Ksp22I TGATCA 1 cut(s) 1291
Kzo9I GATC 6 cut(s) 243, 504, 635, 736, 1260, 1291
LguI GCTCTTC 2 cut(s) 552, 1144
LmnI GCTCC 3 cut(s) 106, 705, 1391
Lsp1109I GCAGC 2 cut(s) 494, 1453
LweI GCATC 3 cut(s) 422, 792, 1432
MaeI CTAG 4 cut(s) 62, 627, 884, 966
MaeIII GTNAC 2 cut(s) 363, 847
MalI GATC 6 cut(s) 245, 506, 637, 738, 1262, 1293
MboI GATC 6 cut(s) 243, 504, 635, 736, 1260, 1291
MflI RGATCY 1 cut(s) 504
MhlI GDGCHC 3 cut(s) 119, 722, 1065
MluCI AATT 9 cut(s) 128, 159, 176, 188, 195, 937, 979, 1037, 1421
MlyI GAGTC 1 cut(s) 1348
MmeI TCCRAC 4 cut(s) 303, 683, 745, 1440
MnlI CCTC 8 cut(s) 143, 248, 300, 364, 808, 1184, 1344, 1456
Mph1103I ATGCAT 2 cut(s) 1436, 1447
MroXI GAANNNNTTC 1 cut(s) 1138
MseI TTAA 4 cut(s) 198, 225, 1302, 1474
MslI CAYNNNNRTG 4 cut(s) 336, 893, 1229, 1256
MspA1I CMGCKG 1 cut(s) 485
MspI CCGG 4 cut(s) 17, 53, 110, 1058
MspR9I CCNGG 2 cut(s) 53, 1058
MvnI CGCG 1 cut(s) 58
MwoI GCNNNNNNNGC 2 cut(s) 939, 1391
NciI CCSGG 2 cut(s) 53, 1058
NdeII GATC 6 cut(s) 243, 504, 635, 736, 1260, 1291
NlaIV GGNNCC 5 cut(s) 108, 889, 1011, 1062, 1151
NmuCI GTSAC 2 cut(s) 363, 847
NsiI ATGCAT 2 cut(s) 1436, 1447
NspI RCATGY 5 cut(s) 341, 904, 1055, 1177, 1434
PaeI GCATGC 1 cut(s) 1434
PciI ACATGT 1 cut(s) 1173
PciSI GCTCTTC 2 cut(s) 552, 1144
PdmI GAANNNNTTC 1 cut(s) 1138
PfeI GAWTC 5 cut(s) 182, 203, 371, 1308, 1412
PkrI GCNGC 3 cut(s) 60, 484, 1468
PleI GAGTC 1 cut(s) 1347
PpsI GAGTC 1 cut(s) 1347
PscI ACATGT 1 cut(s) 1173
PspN4I GGNNCC 5 cut(s) 108, 889, 1011, 1062, 1151
PspPI GGNCC 2 cut(s) 1193, 1265
PstNI CAGNNNCTG 1 cut(s) 1208
PsuI RGATCY 1 cut(s) 504
PvuII CAGCTG 1 cut(s) 485
RsaI GTAC 4 cut(s) 467, 657, 899, 1151
RsaNI GTAC 4 cut(s) 466, 656, 898, 1150
RseI CAYNNNNRTG 4 cut(s) 336, 893, 1229, 1256
SapI GCTCTTC 2 cut(s) 552, 1144
SaqAI TTAA 4 cut(s) 198, 225, 1302, 1474
SatI GCNGC 3 cut(s) 59, 483, 1467
Sau3AI GATC 6 cut(s) 243, 504, 635, 736, 1260, 1291
Sau96I GGNCC 2 cut(s) 1193, 1265
SchI GAGTC 1 cut(s) 1348
ScrFI CCNGG 2 cut(s) 53, 1058
SduI GDGCHC 3 cut(s) 119, 722, 1065
SfaNI GCATC 3 cut(s) 422, 792, 1432
SmiMI CAYNNNNRTG 4 cut(s) 336, 893, 1229, 1256
SmlI CTYRAG 1 cut(s) 1237
SmoI CTYRAG 1 cut(s) 1237
SpeI ACTAGT 1 cut(s) 626
SphI GCATGC 1 cut(s) 1434
Sse9I AATT 9 cut(s) 128, 159, 176, 188, 195, 937, 979, 1037, 1421
SsiI CCGC 2 cut(s) 58, 869
SspI AATATT 1 cut(s) 498
SspMI CTAG 4 cut(s) 62, 627, 884, 966
StyD4I CCNGG 2 cut(s) 51, 1056
StyI CCWWGG 2 cut(s) 7, 517
TaaI ACNGT 4 cut(s) 140, 686, 753, 1204
TaqI TCGA 3 cut(s) 634, 739, 1070
TasI AATT 9 cut(s) 128, 159, 176, 188, 195, 937, 979, 1037, 1421
TatI WGTACW 1 cut(s) 897
TauI GCSGC 1 cut(s) 61
TfiI GAWTC 5 cut(s) 182, 203, 371, 1308, 1412
Tru1I TTAA 4 cut(s) 198, 225, 1302, 1474
Tru9I TTAA 4 cut(s) 198, 225, 1302, 1474
TscAI CASTG 2 cut(s) 305, 1275
TseFI GTSAC 2 cut(s) 363, 847
TseI GCWGC 2 cut(s) 482, 1466
Tsp45I GTSAC 2 cut(s) 363, 847
TspDTI ATGAA 7 cut(s) 300, 443, 636, 926, 1208, 1247, 1396
TspGWI ACGGA 1 cut(s) 157
TspRI CASTG 2 cut(s) 305, 1275
XceI RCATGY 5 cut(s) 341, 904, 1055, 1177, 1434
XcmI CCANNNNNNNNNTGG 1 cut(s) 515
XmnI GAANNNNTTC 1 cut(s) 1138
XspI CTAG 4 cut(s) 62, 627, 884, 966
Zsp2I ATGCAT 2 cut(s) 1436, 1447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.