Rroxscaffold_7G00200710

O-acyltransferase (WSD1-like)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000634
Physical Location & Seq
Reverse (-)
48069891 .. 48074260
4370 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00200710.1

Sequence Viewer

Length: 1635 bp
ATGGGAACGATCGGCCCGTGCATCGCCGGAAGTCGTTTTCCGCCGGTCCGATCGGAGGAACCGGAGCGCCTTCGATCCGGCTCGCAGCTGCGGCGGGGCAAGGAAACACCACAGGGAGCTTGGGCGGGGCTGTGCGAATCCGGAGGGGGCCGGTCCCGTGCGGAGGAGGCGGAGGGCGGCGAAAATTCGGGTCGGTTCGAGCTTGACCGCCGAAGAAGGACTGCAGCCGGTCCTCTCGGTCAGTACTTCACTAGCTCTGTGTTATCGGTATCGAATCTTGGTGTTTTGGAAACTGAGGTTCCAATCGTTGAGCTTCCAGCTATGTCGTTGCTTCAAAATGTGTTCCTCCCCATCAGCCCACGGTTCTCCTCGATCATGGTTGAGAGAAGGGGAAAGAAGCAGTGGAAAAGGGTTGAAGTGAAGCTTGAAGACCATGTTAACATCCCTATTTTCCCTTCTGGCCTATCGCCTGCATCATACGATAAGTATCTTGATGACTACATATCCAAGTTAGCAATGGAAAGACTTCCACAACACAAACCTCTCTGGGAAATTCACATTATCAAGTACCCAACTACCAATGCAGCCGGCACTGTAATATTCAAGCTTCACCAAGCGCTTGGTGATGGCTTCTCTCTCATGGGTGCTCTTCTCTCTTGTTTACAACGAGCTGACAATCCTTCTCTTCCCCTAACCTTCCCGTCAAGAAGGAGGTCGCAGCCATGGAAGAGTGAAAATTTTGTGACTAAAGTTTTTTCTTCTGTCTTCAACACCATATCGGATTTTTTAAATAGCAACTTGGAGGAAGATGATCAAACACCAATCAGATCGGGGAATGATGGAATTGAGTTCAAGCCAATTGCAGTATCAACTATGACGATTTCCCTTGATAGAATCAAACTAATCAAGAGCAGGCTCGGAGTGACGATAAATGACGTTCTTACCGCGATGATCTTCCTTGGTACTCGACTATACATCCAAGAGACGAACAAAAGTTTAAGCAAAGCAAGAGGCACAGCAATTGTATTGCTCAACACAAGGATGATGGGGAATTACACCTCAGTTCAGGAGATGATCAAACCTAATAGCAAGTTGCCATGGGGGAACCATTTGGCACTGTTGCATGTACCAATACCCAAGTTGTTAACGAATAGTGAAGAACAATTTTCAGATAATGCGCTTGACTTTGTCTGGAAGGCACATAAAATTATAAGGAGCAAAAGACATTCTTTGGGTATTCATCTCACTGCTGGGTTCTTGGAGATTCTCAACAAATTTGGAGGCCATGAGGCAGCAGCAAGATACATCCGTAACACATTGAAGAAGTCAAGCATGATAATTTCCAATATGATTGGACCGGTGGAACAGGTGTCTTTGGCAAATCATCCAGTTAAAGGCTTATACTTTTTGGTCTTTGGTTCACCTGAGGGCCTTGATGTAACAATAGTAAGTTATATGGGAAAGGTGAGGATTGCCTTCAAGATGGAAAAGGGTCTTATTGATCCACCAAAGTTCAAGTCATGCATGGAAAAGTCTTATGAGATGATACTGAAGCCTCGGAAAAAACTGCTGCAAAGTTTTCGTGGCAACCATGAACCTGACTTGGACTTCTTGTTTTCTGCCGATTTGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1298 WES_acyltransf

Protein Analysis

544

Amino Acids

60.83

Weight (kDa)

9.57

Isoelectric Point (pI)

48.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WS_DGAT_cat PF03007 134 - 313 3.9e-10 Wax ester synthase/diacylglycerol acyltransferase catalytic domain
WS_DGAT_C PF06974 367 - 516 7.7e-36 WS/DGAT C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000634)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10290 FvH4_3g28230 FvH4_3g28240
malus_domestica MD05G1036400.v1.1 MD10G1159400.v1.1 MD17G1237100.v1.1 MD17G1237200.v1.1 MD17G1237300.v1.1 MD17G1237600.v1.1 MD17G1238200.v1.1
prunus_persica Prupe.3G140400_v2.0.a1 Prupe.3G140700_v2.0.a1 Prupe.3G141100_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G258100_v2.0.a1 Prupe.7G117300_v2.0.a1 Prupe.7G117400_v2.0.a1 Prupe.7G117600_v2.0.a1 Prupe.8G044700_v2.0.a1
pyrus_communis pycom05g02680 pycom17g24220 pycom17g24240
rosa_chinensis RchiOBHm_Chr1g0350141 RchiOBHm_Chr5g0050931 RchiOBHm_Chr5g0051001 RchiOBHm_Chr5g0051021 RchiOBHm_Chr6g0267491
rosa_laevigata RLG00000013999 RLG00000028502 RLG00000034744 RLG00000034748 RLG00000034753
rosa_multiflora Rmu_sc0000446.1_g000056 Rmu_sc0000782.1_g000006 Rmu_sc0002951.1_g000001 Rmu_sc0003674.1_g000026 Rmu_sc0003756.1_g000004 Rmu_sc0011917.1_g000004 Rmu_sc0012005.1_g000019 Rmu_sc0040739.1_g000001
rosa_roxburghii Rroxscaffold_1G00029730 Rroxscaffold_1G00029740 Rroxscaffold_4G00305060 Rroxscaffold_7G00200710
rosa_rugosa Rorug01G0208400 Rorug05G0265200.1 Rorug05G0265300.1 Rorug05G0265400.1 Rorug05G0265500.1 Rorug05G0294000 Rorug06G0033100 Rorug06G0243300
rosa_samantha Rh1BG192100 Rh1BG192200 Rh1DG220600 Rh5AG270300 Rh5AG337200 Rh5AG337700 Rh5BG346700 Rh5BG347400 Rh5CG374300 Rh5DG359700 Rh5DG360500 Rh6AG154500 Rh6BG155100 Rh6BG362300 Rh6CG150300 Rh6DG140300 Rh6DG356300
rosa_wichuraiana Rw0G018820 Rw1G019350 Rw5G025300 Rw5G031770 Rw5G031820 Rw5G031870 Rw5G031910 Rw6G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1211
AccII CGCG 1 cut(s) 947
AccIII TCCGGA 1 cut(s) 140
AciI CCGC 9 cut(s) 41, 91, 94, 125, 161, 170, 177, 208, 945
AclWI GGATC 2 cut(s) 69, 1496
AcsI RAATTY 4 cut(s) 184, 552, 736, 1274
AcuI CTGAAG 1 cut(s) 1571
AfaI GTAC 4 cut(s) 245, 569, 964, 1128
AfeI AGCGCT 1 cut(s) 618
AfiI CCNNNNNNNGG 3 cut(s) 55, 163, 1394
AgeI ACCGGT 1 cut(s) 1357
AgsI TTSAA 9 cut(s) 335, 416, 428, 604, 769, 853, 1321, 1480, 1516
AluBI AGCT 9 cut(s) 88, 119, 202, 255, 313, 320, 424, 607, 671
AluI AGCT 9 cut(s) 88, 119, 202, 255, 313, 320, 424, 607, 671
Alw21I GWGCWC 1 cut(s) 649
Alw26I GTCTC 1 cut(s) 977
AlwI GGATC 2 cut(s) 69, 1496
Aor13HI TCCGGA 1 cut(s) 140
Aor51HI AGCGCT 1 cut(s) 618
AoxI GGCC 5 cut(s) 13, 148, 460, 1282, 1429
ApeKI GCWGC 8 cut(s) 85, 88, 224, 584, 718, 1292, 1295, 1570
ApoI RAATTY 4 cut(s) 184, 552, 736, 1274
ArsI GACNNNNNNTTYG 2 cut(s) 1502, 1534
AsiGI ACCGGT 1 cut(s) 1357
Asp700I GAANNNNTTC 1 cut(s) 525
AspLEI GCGC 3 cut(s) 69, 619, 1180
AspS9I GGNCC 7 cut(s) 14, 46, 148, 153, 230, 1355, 1429
AsuHPI GGTGA 4 cut(s) 602, 635, 1413, 1477
AvaII GGWCC 4 cut(s) 46, 153, 230, 1355
AxyI CCTNAGG 1 cut(s) 1425
BaeI ACNNNNGTAYC 2 cut(s) 1294, 1327
BbsI GAAGAC 2 cut(s) 435, 757
Bbv12I GWGCWC 1 cut(s) 649
BbvI GCAGC 8 cut(s) 75, 97, 236, 596, 730, 1304, 1307, 1557
BccI CCATC 5 cut(s) 359, 620, 833, 1039, 1477
BcgI CGANNNNNNTGC 2 cut(s) 1562, 1596
BclI TGATCA 2 cut(s) 811, 1074
BcoDI GTCTC 1 cut(s) 977
BfaI CTAG 1 cut(s) 252
BfmI CTRYAG 1 cut(s) 222
BfoI RGCGCY 2 cut(s) 70, 620
BmcAI AGTACT 1 cut(s) 245
Bme18I GGWCC 4 cut(s) 46, 153, 230, 1355
BmgT120I GGNCC 7 cut(s) 14, 46, 148, 153, 230, 1355, 1429
BmiI GGNNCC 5 cut(s) 60, 149, 155, 300, 1106
BmsI GCATC 2 cut(s) 30, 482
BpiI GAAGAC 2 cut(s) 435, 757
BsaBI GATNNNNATC 1 cut(s) 486
BsaJI CCNNGG 5 cut(s) 359, 722, 958, 1097, 1556
BsaWI WCCGGW 3 cut(s) 61, 140, 1357
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 163, 1394
Bse118I RCCGGY 5 cut(s) 43, 150, 227, 587, 1357
Bse1I ACTGG 1 cut(s) 1388
Bse21I CCTNAGG 1 cut(s) 1425
Bse3DI GCAATG 1 cut(s) 522
Bse8I GATNNNNATC 1 cut(s) 486
BseAI TCCGGA 1 cut(s) 140
BseDI CCNNGG 5 cut(s) 359, 722, 958, 1097, 1556
BseGI GGATG 5 cut(s) 441, 975, 1047, 1305, 1384
BseJI GATNNNNATC 1 cut(s) 486
BseLI CCNNNNNNNGG 3 cut(s) 55, 163, 1394
BseMI GCAATG 1 cut(s) 522
BseMII CTCAG 3 cut(s) 285, 1074, 1416
BseNI ACTGG 1 cut(s) 1388
BseRI GAGGAG 2 cut(s) 179, 358
BseXI GCAGC 8 cut(s) 75, 97, 236, 596, 730, 1304, 1307, 1557
BseYI CCCAGC 1 cut(s) 1250
Bsh1236I CGCG 1 cut(s) 947
Bsh1285I CGRYCG 2 cut(s) 12, 53
BshFI GGCC 5 cut(s) 15, 150, 462, 1284, 1431
BshTI ACCGGT 1 cut(s) 1357
BsiEI CGRYCG 2 cut(s) 12, 53
BsiHKAI GWGCWC 1 cut(s) 649
BsiSI CCGG 9 cut(s) 27, 44, 62, 78, 141, 151, 228, 588, 1358
BslFI GGGAC 1 cut(s) 139
BslI CCNNNNNNNGG 3 cut(s) 55, 163, 1394
BsmAI GTCTC 1 cut(s) 977
BsmBI CGTCTC 1 cut(s) 977
BsmFI GGGAC 1 cut(s) 139
BsnI GGCC 5 cut(s) 15, 150, 462, 1284, 1431
Bsp1286I GDGCHC 1 cut(s) 649
Bsp13I TCCGGA 1 cut(s) 140
Bsp143I GATC 9 cut(s) 9, 50, 74, 372, 811, 827, 951, 1074, 1501
Bsp19I CCATGG 2 cut(s) 722, 1097
BspACI CCGC 9 cut(s) 41, 91, 94, 125, 161, 170, 177, 208, 945
BspANI GGCC 5 cut(s) 15, 150, 462, 1284, 1431
BspCNI CTCAG 3 cut(s) 286, 1073, 1417
BspEI TCCGGA 1 cut(s) 140
BspFNI CGCG 1 cut(s) 947
BspLI GGNNCC 5 cut(s) 60, 149, 155, 300, 1106
BspMAI CTGCAG 1 cut(s) 226
BspPI GGATC 2 cut(s) 69, 1496
BspQI GCTCTTC 1 cut(s) 654
BsrDI GCAATG 1 cut(s) 522
BsrFI RCCGGY 5 cut(s) 43, 150, 227, 587, 1357
BsrI ACTGG 1 cut(s) 1388
BssAI RCCGGY 5 cut(s) 43, 150, 227, 587, 1357
BssECI CCNNGG 5 cut(s) 359, 722, 958, 1097, 1556
BssMI GATC 9 cut(s) 9, 50, 74, 372, 811, 827, 951, 1074, 1501
BssT1I CCWWGG 3 cut(s) 722, 958, 1097
Bst4CI ACNGT 3 cut(s) 363, 595, 1119
Bst6I CTCTTC 3 cut(s) 654, 690, 722
BstC8I GCNNGC 4 cut(s) 83, 471, 589, 914
BstDEI CTNAG 3 cut(s) 294, 1060, 1425
BstDSI CCRYGG 3 cut(s) 359, 722, 1097
BstF5I GGATG 5 cut(s) 441, 975, 1047, 1305, 1384
BstFNI CGCG 1 cut(s) 947
BstH2I RGCGCY 2 cut(s) 70, 620
BstHHI GCGC 3 cut(s) 69, 619, 1180
BstKTI GATC 9 cut(s) 12, 53, 77, 375, 814, 830, 954, 1077, 1504
BstMAI GTCTC 1 cut(s) 977
BstMBI GATC 9 cut(s) 9, 50, 74, 372, 811, 827, 951, 1074, 1501
BstMCI CGRYCG 2 cut(s) 12, 53
BstMWI GCNNNNNNNGC 2 cut(s) 91, 167
BstNSI RCATGY 1 cut(s) 1127
BstSFI CTRYAG 1 cut(s) 222
BstUI CGCG 1 cut(s) 947
BstV1I GCAGC 8 cut(s) 75, 97, 236, 596, 730, 1304, 1307, 1557
BstV2I GAAGAC 2 cut(s) 435, 757
BstXI CCANNNNNNTGG 1 cut(s) 620
Bsu36I CCTNAGG 1 cut(s) 1425
BsuRI GGCC 5 cut(s) 15, 150, 462, 1284, 1431
BtgI CCRYGG 3 cut(s) 359, 722, 1097
BtgZI GCGATG 2 cut(s) 7, 962
BtsCI GGATG 5 cut(s) 441, 975, 1047, 1305, 1384
BtsI GCAGTG 2 cut(s) 407, 1245
BtsIMutI CAGTG 4 cut(s) 407, 591, 1115, 1245
Cac8I GCNNGC 4 cut(s) 83, 471, 589, 914
CfoI GCGC 3 cut(s) 69, 619, 1180
Cfr10I RCCGGY 5 cut(s) 43, 150, 227, 587, 1357
Cfr13I GGNCC 7 cut(s) 14, 46, 148, 153, 230, 1355, 1429
CpoI CGGWCCG 1 cut(s) 46
Csp6I GTAC 4 cut(s) 244, 568, 963, 1127
CspAI ACCGGT 1 cut(s) 1357
CspI CGGWCCG 1 cut(s) 46
CviQI GTAC 4 cut(s) 244, 568, 963, 1127
DdeI CTNAG 3 cut(s) 294, 1060, 1425
DpnI GATC 9 cut(s) 11, 52, 76, 374, 813, 829, 953, 1076, 1503
DpnII GATC 9 cut(s) 9, 50, 74, 372, 811, 827, 951, 1074, 1501
DraI TTTAAA 1 cut(s) 789
Eam1104I CTCTTC 3 cut(s) 654, 690, 722
EarI CTCTTC 3 cut(s) 654, 690, 722
EciI GGCGGA 2 cut(s) 30, 185
Eco130I CCWWGG 3 cut(s) 722, 958, 1097
Eco47I GGWCC 4 cut(s) 46, 153, 230, 1355
Eco47III AGCGCT 1 cut(s) 618
Eco57I CTGAAG 1 cut(s) 1571
Eco81I CCTNAGG 1 cut(s) 1425
EcoO109I RGGNCCY 1 cut(s) 1429
EcoT14I CCWWGG 3 cut(s) 722, 958, 1097
EcoT22I ATGCAT 1 cut(s) 1526
ErhI CCWWGG 3 cut(s) 722, 958, 1097
Esp3I CGTCTC 1 cut(s) 977
FalI AAGNNNNNCTT 4 cut(s) 408, 440, 1213, 1245
FaqI GGGAC 1 cut(s) 139
FauI CCCGC 2 cut(s) 87, 118
FbaI TGATCA 2 cut(s) 811, 1074
FokI GGATG 5 cut(s) 428, 962, 1054, 1292, 1371
FspBI CTAG 1 cut(s) 252
GlaI GCGC 3 cut(s) 68, 618, 1179
GsaI CCCAGC 1 cut(s) 1254
HaeII RGCGCY 2 cut(s) 70, 620
HaeIII GGCC 5 cut(s) 15, 150, 462, 1284, 1431
HapII CCGG 9 cut(s) 27, 44, 62, 78, 141, 151, 228, 588, 1358
HhaI GCGC 3 cut(s) 69, 619, 1180
Hin6I GCGC 3 cut(s) 67, 617, 1178
HinP1I GCGC 3 cut(s) 67, 617, 1178
HincII GTYRAC 2 cut(s) 439, 1146
HindII GTYRAC 2 cut(s) 439, 1146
HindIII AAGCTT 2 cut(s) 422, 605
HinfI GANTC 4 cut(s) 137, 274, 894, 1264
HpaI GTTAAC 2 cut(s) 439, 1146
HpaII CCGG 9 cut(s) 27, 44, 62, 78, 141, 151, 228, 588, 1358
HphI GGTGA 4 cut(s) 602, 635, 1413, 1477
Hpy166II GTNNAC 4 cut(s) 439, 662, 1146, 1421
Hpy188I TCNGA 7 cut(s) 50, 55, 781, 827, 920, 1171, 1560
Hpy188III TCNNGA 7 cut(s) 141, 491, 705, 907, 1067, 1192, 1480
Hpy8I GTNNAC 4 cut(s) 439, 662, 1146, 1421
HpyAV CCTTC 9 cut(s) 80, 210, 381, 465, 690, 702, 706, 1189, 1486
HpyCH4III ACNGT 3 cut(s) 363, 595, 1119
HpyCH4IV ACGT 1 cut(s) 936
HpyCH4V TGCA 8 cut(s) 21, 224, 473, 584, 863, 1123, 1524, 1573
HpyF10VI GCNNNNNNNGC 2 cut(s) 91, 167
HpyF3I CTNAG 3 cut(s) 294, 1060, 1425
HpySE526I ACGT 1 cut(s) 936
HspAI GCGC 3 cut(s) 67, 617, 1178
Kpn2I TCCGGA 1 cut(s) 140
KroI GCCGGC 1 cut(s) 587
KroNI GCCGGC 1 cut(s) 589
Ksp22I TGATCA 2 cut(s) 811, 1074
KspAI GTTAAC 2 cut(s) 439, 1146
Kzo9I GATC 9 cut(s) 9, 50, 74, 372, 811, 827, 951, 1074, 1501
LguI GCTCTTC 1 cut(s) 654
LmnI GCTCC 3 cut(s) 64, 116, 1215
Lsp1109I GCAGC 8 cut(s) 75, 97, 236, 596, 730, 1304, 1307, 1557
LweI GCATC 2 cut(s) 30, 482
MaeI CTAG 1 cut(s) 252
MaeII ACGT 1 cut(s) 936
MaeIII GTNAC 4 cut(s) 742, 922, 1310, 1438
MalI GATC 9 cut(s) 11, 52, 76, 374, 813, 829, 953, 1076, 1503
MboI GATC 9 cut(s) 9, 50, 74, 372, 811, 827, 951, 1074, 1501
MfeI CAATTG 2 cut(s) 858, 1020
MhlI GDGCHC 1 cut(s) 649
Mph1103I ATGCAT 1 cut(s) 1526
MroI TCCGGA 1 cut(s) 140
MroNI GCCGGC 1 cut(s) 587
MroXI GAANNNNTTC 1 cut(s) 525
MseI TTAA 5 cut(s) 438, 788, 998, 1145, 1392
MslI CAYNNNNRTG 1 cut(s) 1040
MspA1I CMGCKG 1 cut(s) 88
MspI CCGG 9 cut(s) 27, 44, 62, 78, 141, 151, 228, 588, 1358
MunI CAATTG 2 cut(s) 858, 1020
MvnI CGCG 1 cut(s) 947
MwoI GCNNNNNNNGC 2 cut(s) 91, 167
NaeI GCCGGC 1 cut(s) 589
NcoI CCATGG 2 cut(s) 722, 1097
NdeII GATC 9 cut(s) 9, 50, 74, 372, 811, 827, 951, 1074, 1501
NgoMIV GCCGGC 1 cut(s) 587
NlaIV GGNNCC 5 cut(s) 60, 149, 155, 300, 1106
NmuCI GTSAC 2 cut(s) 742, 922
NsiI ATGCAT 1 cut(s) 1526
NspI RCATGY 1 cut(s) 1127
PciSI GCTCTTC 1 cut(s) 654
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PdiI GCCGGC 1 cut(s) 589
PdmI GAANNNNTTC 1 cut(s) 525
PfeI GAWTC 4 cut(s) 137, 274, 894, 1264
PflFI GACNNNGTC 1 cut(s) 1187
PinAI ACCGGT 1 cut(s) 1357
Ple19I CGATCG 2 cut(s) 12, 53
PsiI TTATAA 1 cut(s) 1211
PspFI CCCAGC 1 cut(s) 1250
PspN4I GGNNCC 5 cut(s) 60, 149, 155, 300, 1106
PspPI GGNCC 7 cut(s) 14, 46, 148, 153, 230, 1355, 1429
PstI CTGCAG 1 cut(s) 226
PsyI GACNNNGTC 1 cut(s) 1187
PvuI CGATCG 2 cut(s) 12, 53
PvuII CAGCTG 1 cut(s) 88
RsaI GTAC 4 cut(s) 245, 569, 964, 1128
RsaNI GTAC 4 cut(s) 244, 568, 963, 1127
RseI CAYNNNNRTG 1 cut(s) 1040
Rsr2I CGGWCCG 1 cut(s) 46
RsrII CGGWCCG 1 cut(s) 46
SapI GCTCTTC 1 cut(s) 654
SaqAI TTAA 5 cut(s) 438, 788, 998, 1145, 1392
Sau3AI GATC 9 cut(s) 9, 50, 74, 372, 811, 827, 951, 1074, 1501
Sau96I GGNCC 7 cut(s) 14, 46, 148, 153, 230, 1355, 1429
ScaI AGTACT 1 cut(s) 245
SduI GDGCHC 1 cut(s) 649
SfaNI GCATC 2 cut(s) 30, 482
SfcI CTRYAG 1 cut(s) 222
SinI GGWCC 4 cut(s) 46, 153, 230, 1355
SmiMI CAYNNNNRTG 1 cut(s) 1040
SsiI CCGC 9 cut(s) 41, 91, 94, 125, 161, 170, 177, 208, 945
SspI AATATT 1 cut(s) 600
SspMI CTAG 1 cut(s) 252
StyI CCWWGG 3 cut(s) 722, 958, 1097
TaaI ACNGT 3 cut(s) 363, 595, 1119
TaiI ACGT 1 cut(s) 939
TaqI TCGA 5 cut(s) 73, 198, 272, 371, 967
TaqII GACCGA 1 cut(s) 227
TatI WGTACW 1 cut(s) 243
TauI GCSGC 2 cut(s) 94, 180
TfiI GAWTC 4 cut(s) 137, 274, 894, 1264
Tru1I TTAA 5 cut(s) 438, 788, 998, 1145, 1392
Tru9I TTAA 5 cut(s) 438, 788, 998, 1145, 1392
TscAI CASTG 4 cut(s) 407, 598, 1122, 1252
TseFI GTSAC 2 cut(s) 742, 922
TseI GCWGC 8 cut(s) 85, 88, 224, 584, 718, 1292, 1295, 1570
Tsp45I GTSAC 2 cut(s) 742, 922
TspDTI ATGAA 2 cut(s) 1229, 1608
TspGWI ACGGA 1 cut(s) 1298
TspRI CASTG 4 cut(s) 407, 598, 1122, 1252
Tth111I GACNNNGTC 1 cut(s) 1187
VpaK11BI GGWCC 4 cut(s) 46, 153, 230, 1355
XapI RAATTY 4 cut(s) 184, 552, 736, 1274
XceI RCATGY 1 cut(s) 1127
XcmI CCANNNNNNNNNTGG 2 cut(s) 117, 514
XmnI GAANNNNTTC 1 cut(s) 525
XspI CTAG 1 cut(s) 252
ZrmI AGTACT 1 cut(s) 245
Zsp2I ATGCAT 1 cut(s) 1526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.