pycom05g02680

O-acyltransferase (WSD1-like)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000634
Physical Location & Seq
Forward (+)
3165669 .. 3167860
2192 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g02680.2

Sequence Viewer

Length: 1497 bp
ATGGAGCACTTGGAGGAAGATGAAGTAGTAGTACTGGAGCCAGTGAGCCCTACTGCTCAGTACTTCACTAGCTCTGTCCTGTCCGTATCAAATATTGGCGTTATGGAACTCGAAAATCCACTGAGTGAGTCTCAAGCAATCTCATTACTTAAGACTCTCTTCCTCCCCATCAGCCCGCGCTTCTCCTCTATTGTGGTTGAAATCAATGGAAAGAAACGATGGAAAAGGGTTGAAGTGAAGCTTGAAGAGCACATTAGCGTCCCTGTTATTCCTTCTAACTTATCAACTGAATCATATGAAAAGTATCTTGATGGCTACATATCAAAGCTAGCACTAGAAAGAGTGTCACAAGACAAACCCTTATGGGAAATTCATATCCTCAATTACCCAACAAGAAATAATAACGCTGCTTGCAACCTTATATTCAAGCTCCATCACGCCCTCGGTGATGGCTTCTCTCTCATGAGTGCCATTCTTTCTTGTCTACGGAGAGCCGACAACCCTTCTCTTCCCCTAACATTTCCTTCAAGGCAGAGGTCACAGCAAATGAGTGATGAAAGCTTTTTGTCTAGAACTATCTCATCAGTCTTCAACACTCTTTCAGATATTTGGAGGAGCACCGTGGGGGAAGATGATCTATCACCAATTAGGTCTGGCAATGGAATTGAGTTTCAGCCCATTACAGTGGCAACTATGACTTTCTCTCTTGACCAAATCAAATCAATCAAGAGTAAGCTTGGAGTGACGGTAAACGATGTTCTTGCCGGTATGATCTTCCTTGGCACTCGACTATACATGCAAGAGATAAACAGAAGTTCAAGCAAAGCAAGAGGCACGGCAGTGGTACTGCTGAACACGAGAATGATGGGGAAGTATACTTCAATCCAGGAGATGATGAAACCCGACAGCAAGATGCCATGGGGGAATCATTTTACGTTCTTGCATGTACCAATCCCCAATTTATCATTACCCGAAGATCACGATCATGATTTTCCCGCTGATCAATATTCAAATGCCCTTGACTTTGTCTGGGAAGCACAAAAAATAATCACTAGGAAGAGAAGTTCTTTAGCTATTTATCTCACTTGTAGGTTCTTGGAGTTCCTAAACAAATTTGGAGGCCATGAGGTTTGTGATAGAGCAGCAGCCAGATACATCCACAGCACACTGAAGAACACGAGCATGATGATCTCAAATTTGATTGGTCCTGTGGAACAAATGGCTTTAGCTAATAATCCAGTTAAAGGAATGTACTTTCTGGTGTTTGGTTCACCTGAGAAATTATATGACATGGTGTTAATGGTATTGCAGGGGCTTGACGTAACAATAGTAAGTTATATGGGAAAGGTGAGGGTTGCCTTCAAAATGGAAAAGGGCCTCATTGAACCACAAAAGTTCAAGTCATGCATGCAAAATGCATTTGAGATGATACGCAAAGCCTCCGACGAATATCCTATTCCTACGCAGAAAAACTCAAAAACAATAAAACATCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1298 WES_acyltransf

Protein Analysis

499

Amino Acids

56.33

Weight (kDa)

8.12

Isoelectric Point (pI)

51.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000634)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10290 FvH4_3g28230 FvH4_3g28240
malus_domestica MD05G1036400.v1.1 MD10G1159400.v1.1 MD17G1237100.v1.1 MD17G1237200.v1.1 MD17G1237300.v1.1 MD17G1237600.v1.1 MD17G1238200.v1.1
prunus_persica Prupe.3G140400_v2.0.a1 Prupe.3G140700_v2.0.a1 Prupe.3G141100_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G258100_v2.0.a1 Prupe.7G117300_v2.0.a1 Prupe.7G117400_v2.0.a1 Prupe.7G117600_v2.0.a1 Prupe.8G044700_v2.0.a1
pyrus_communis pycom05g02680 pycom17g24220 pycom17g24240
rosa_chinensis RchiOBHm_Chr1g0350141 RchiOBHm_Chr5g0050931 RchiOBHm_Chr5g0051001 RchiOBHm_Chr5g0051021 RchiOBHm_Chr6g0267491
rosa_laevigata RLG00000013999 RLG00000028502 RLG00000034744 RLG00000034748 RLG00000034753
rosa_multiflora Rmu_sc0000446.1_g000056 Rmu_sc0000782.1_g000006 Rmu_sc0002951.1_g000001 Rmu_sc0003674.1_g000026 Rmu_sc0003756.1_g000004 Rmu_sc0011917.1_g000004 Rmu_sc0012005.1_g000019 Rmu_sc0040739.1_g000001
rosa_roxburghii Rroxscaffold_1G00029730 Rroxscaffold_1G00029740 Rroxscaffold_4G00305060 Rroxscaffold_7G00200710
rosa_rugosa Rorug01G0208400 Rorug05G0265200.1 Rorug05G0265300.1 Rorug05G0265400.1 Rorug05G0265500.1 Rorug05G0294000 Rorug06G0033100 Rorug06G0243300
rosa_samantha Rh1BG192100 Rh1BG192200 Rh1DG220600 Rh5AG270300 Rh5AG337200 Rh5AG337700 Rh5BG346700 Rh5BG347400 Rh5CG374300 Rh5DG359700 Rh5DG360500 Rh6AG154500 Rh6BG155100 Rh6BG362300 Rh6CG150300 Rh6DG140300 Rh6DG356300
rosa_wichuraiana Rw0G018820 Rw1G019350 Rw5G025300 Rw5G031770 Rw5G031820 Rw5G031870 Rw5G031910 Rw6G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 484, 875
AccII CGCG 1 cut(s) 178
AciI CCGC 2 cut(s) 176, 996
AcsI RAATTY 3 cut(s) 369, 1112, 1195
AcuI CTGAAG 1 cut(s) 1190
AdeI CACNNNGTG 1 cut(s) 125
AfaI GTAC 5 cut(s) 33, 62, 846, 948, 1253
AfiI CCNNNNNNNGG 1 cut(s) 1244
AflII CTTAAG 1 cut(s) 149
AjnI CCWGG 1 cut(s) 885
AleI CACNNNNGTG 1 cut(s) 839
AloI GAACNNNNNNTCC 2 cut(s) 1048, 1080
AluBI AGCT 8 cut(s) 72, 241, 328, 430, 561, 736, 1073, 1229
AluI AGCT 8 cut(s) 72, 241, 328, 430, 561, 736, 1073, 1229
Alw21I GWGCWC 3 cut(s) 9, 252, 620
Alw26I GTCTC 1 cut(s) 135
AoxI GGCC 2 cut(s) 1120, 1375
ApeKI GCWGC 3 cut(s) 407, 1142, 1145
ApoI RAATTY 3 cut(s) 369, 1112, 1195
AspLEI GCGC 1 cut(s) 180
AspS9I GGNCC 2 cut(s) 1205, 1375
AsuHPI GGTGA 4 cut(s) 458, 633, 1263, 1360
AsuNHI GCTAGC 1 cut(s) 328
AvaII GGWCC 1 cut(s) 1205
BanII GRGCYC 1 cut(s) 50
BarI GAAGNNNNNNTAC 2 cut(s) 15, 47
BauI CACGAG 2 cut(s) 856, 1177
BbsI GAAGAC 1 cut(s) 580
Bbv12I GWGCWC 3 cut(s) 9, 252, 620
BbvI GCAGC 3 cut(s) 394, 1154, 1157
BccI CCATC 6 cut(s) 176, 213, 305, 441, 443, 859
BceAI ACGGC 1 cut(s) 852
BcgI CGANNNNNNTGC 2 cut(s) 743, 777
BciT130I CCWGG 1 cut(s) 887
BclI TGATCA 1 cut(s) 1000
BcoDI GTCTC 1 cut(s) 135
BfaI CTAG 5 cut(s) 69, 329, 335, 570, 1053
BfrI CTTAAG 1 cut(s) 149
BisI GCNGC 3 cut(s) 408, 1143, 1146
BlsI GCNGC 3 cut(s) 409, 1144, 1147
BmcAI AGTACT 2 cut(s) 33, 62
Bme1390I CCNGG 1 cut(s) 887
Bme18I GGWCC 1 cut(s) 1205
BmgT120I GGNCC 2 cut(s) 1205, 1375
BmiI GGNNCC 1 cut(s) 39
BmrFI CCNGG 1 cut(s) 887
BmsI GCATC 1 cut(s) 903
BmtI GCTAGC 1 cut(s) 332
BpiI GAAGAC 1 cut(s) 580
BplI GAGNNNNNCTC 2 cut(s) 115, 147
BpmI CTGGAG 1 cut(s) 56
BpuEI CTTGAG 1 cut(s) 117
BsaBI GATNNNNATC 1 cut(s) 981
BsaJI CCNNGG 4 cut(s) 442, 621, 778, 917
BsaXI ACNNNNNCTCC 2 cut(s) 732, 762
Bsc4I CCNNNNNNNGG 1 cut(s) 1244
Bse118I RCCGGY 1 cut(s) 764
Bse1I ACTGG 3 cut(s) 39, 41, 1238
Bse3DI GCAATG 1 cut(s) 664
Bse8I GATNNNNATC 1 cut(s) 981
BseBI CCWGG 1 cut(s) 887
BseDI CCNNGG 4 cut(s) 442, 621, 778, 917
BseGI GGATG 1 cut(s) 1155
BseJI GATNNNNATC 1 cut(s) 981
BseLI CCNNNNNNNGG 1 cut(s) 1244
BseMI GCAATG 1 cut(s) 664
BseMII CTCAG 3 cut(s) 71, 113, 1266
BseNI ACTGG 3 cut(s) 39, 41, 1238
BseRI GAGGAG 2 cut(s) 175, 628
BseXI GCAGC 3 cut(s) 394, 1154, 1157
Bsh1236I CGCG 1 cut(s) 178
BshFI GGCC 2 cut(s) 1122, 1377
BsiHKAI GWGCWC 3 cut(s) 9, 252, 620
BsiSI CCGG 1 cut(s) 765
BslFI GGGAC 1 cut(s) 245
BslI CCNNNNNNNGG 1 cut(s) 1244
BsmAI GTCTC 1 cut(s) 135
BsmFI GGGAC 1 cut(s) 245
BsnI GGCC 2 cut(s) 1122, 1377
Bsp1286I GDGCHC 4 cut(s) 9, 50, 252, 620
Bsp143I GATC 6 cut(s) 634, 771, 976, 982, 1000, 1188
Bsp19I CCATGG 1 cut(s) 917
BspACI CCGC 2 cut(s) 176, 996
BspANI GGCC 2 cut(s) 1122, 1377
BspCNI CTCAG 3 cut(s) 70, 114, 1267
BspFNI CGCG 1 cut(s) 178
BspHI TCATGA 2 cut(s) 462, 985
BspLI GGNNCC 1 cut(s) 39
BspOI GCTAGC 1 cut(s) 332
BspQI GCTCTTC 1 cut(s) 240
BspTI CTTAAG 1 cut(s) 149
BsrDI GCAATG 1 cut(s) 664
BsrFI RCCGGY 1 cut(s) 764
BsrI ACTGG 3 cut(s) 39, 41, 1238
BssAI RCCGGY 1 cut(s) 764
BssECI CCNNGG 4 cut(s) 442, 621, 778, 917
BssMI GATC 6 cut(s) 634, 771, 976, 982, 1000, 1188
BssNAI GTATAC 1 cut(s) 876
BssSI CACGAG 2 cut(s) 856, 1177
BssT1I CCWWGG 2 cut(s) 778, 917
Bst1107I GTATAC 1 cut(s) 876
Bst2BI CACGAG 2 cut(s) 856, 1177
Bst2UI CCWGG 1 cut(s) 887
Bst4CI ACNGT 3 cut(s) 622, 685, 748
Bst6I CTCTTC 4 cut(s) 164, 240, 513, 1052
BstAFI CTTAAG 1 cut(s) 149
BstC8I GCNNGC 4 cut(s) 176, 330, 412, 1409
BstDEI CTNAG 3 cut(s) 57, 122, 1275
BstDSI CCRYGG 2 cut(s) 621, 917
BstF5I GGATG 1 cut(s) 1155
BstFNI CGCG 1 cut(s) 178
BstHHI GCGC 1 cut(s) 180
BstKTI GATC 6 cut(s) 637, 774, 979, 985, 1003, 1191
BstMAI GTCTC 1 cut(s) 135
BstMBI GATC 6 cut(s) 634, 771, 976, 982, 1000, 1188
BstMWI GCNNNNNNNGC 1 cut(s) 247
BstNI CCWGG 1 cut(s) 887
BstNSI RCATGY 3 cut(s) 799, 947, 1411
BstSCI CCNGG 1 cut(s) 885
BstUI CGCG 1 cut(s) 178
BstV1I GCAGC 3 cut(s) 394, 1154, 1157
BstV2I GAAGAC 1 cut(s) 580
BstXI CCANNNNNNTGG 1 cut(s) 685
BstZ17I GTATAC 1 cut(s) 876
BsuRI GGCC 2 cut(s) 1122, 1377
BtgI CCRYGG 2 cut(s) 621, 917
BtsCI GGATG 1 cut(s) 1155
BtsI GCAGTG 1 cut(s) 846
BtsIMutI CAGTG 5 cut(s) 48, 119, 690, 846, 1166
Cac8I GCNNGC 4 cut(s) 176, 330, 412, 1409
CciI TCATGA 2 cut(s) 462, 985
CfoI GCGC 1 cut(s) 180
Cfr10I RCCGGY 1 cut(s) 764
Cfr13I GGNCC 2 cut(s) 1205, 1375
CseI GACGC 1 cut(s) 247
Csp6I GTAC 5 cut(s) 32, 61, 845, 947, 1252
CviQI GTAC 5 cut(s) 32, 61, 845, 947, 1252
DdeI CTNAG 3 cut(s) 57, 122, 1275
DpnI GATC 6 cut(s) 636, 773, 978, 984, 1002, 1190
DpnII GATC 6 cut(s) 634, 771, 976, 982, 1000, 1188
DraIII CACNNNGTG 1 cut(s) 125
Eam1104I CTCTTC 4 cut(s) 164, 240, 513, 1052
EarI CTCTTC 4 cut(s) 164, 240, 513, 1052
Eco130I CCWWGG 2 cut(s) 778, 917
Eco24I GRGCYC 1 cut(s) 50
Eco47I GGWCC 1 cut(s) 1205
Eco57I CTGAAG 1 cut(s) 1190
EcoO109I RGGNCCY 1 cut(s) 1375
EcoRII CCWGG 1 cut(s) 885
EcoT14I CCWWGG 2 cut(s) 778, 917
EcoT22I ATGCAT 2 cut(s) 1409, 1420
EcoT38I GRGCYC 1 cut(s) 50
ErhI CCWWGG 2 cut(s) 778, 917
FalI AAGNNNNNCTT 4 cut(s) 143, 175, 225, 257
FaqI GGGAC 1 cut(s) 245
FauI CCCGC 2 cut(s) 183, 1003
FauNDI CATATG 1 cut(s) 295
FbaI TGATCA 1 cut(s) 1000
FblI GTMKAC 2 cut(s) 484, 875
Fnu4HI GCNGC 3 cut(s) 408, 1143, 1146
FokI GGATG 1 cut(s) 1142
FriOI GRGCYC 1 cut(s) 50
Fsp4HI GCNGC 3 cut(s) 408, 1143, 1146
FspBI CTAG 5 cut(s) 69, 329, 335, 570, 1053
GlaI GCGC 1 cut(s) 179
GluI GCNGC 3 cut(s) 408, 1143, 1146
GsuI CTGGAG 1 cut(s) 56
HaeIII GGCC 2 cut(s) 1122, 1377
HapII CCGG 1 cut(s) 765
HgaI GACGC 1 cut(s) 247
HhaI GCGC 1 cut(s) 180
Hin6I GCGC 1 cut(s) 178
HinP1I GCGC 1 cut(s) 178
HindIII AAGCTT 3 cut(s) 239, 559, 734
HinfI GANTC 4 cut(s) 128, 154, 290, 925
HpaII CCGG 1 cut(s) 765
HphI GGTGA 4 cut(s) 458, 633, 1263, 1360
Hpy166II GTNNAC 4 cut(s) 485, 751, 876, 1271
Hpy188I TCNGA 2 cut(s) 604, 1444
Hpy188III TCNNGA 7 cut(s) 308, 463, 570, 707, 727, 980, 986
Hpy8I GTNNAC 4 cut(s) 485, 751, 876, 1271
Hpy99I CGWCG 1 cut(s) 1448
HpyAV CCTTC 4 cut(s) 282, 513, 534, 1369
HpyCH4III ACNGT 3 cut(s) 622, 685, 748
HpyCH4IV ACGT 2 cut(s) 935, 1320
HpyCH4V TGCA 7 cut(s) 414, 799, 943, 1309, 1407, 1411, 1418
HpyF10VI GCNNNNNNNGC 1 cut(s) 247
HpyF3I CTNAG 3 cut(s) 57, 122, 1275
HpySE526I ACGT 2 cut(s) 935, 1320
HspAI GCGC 1 cut(s) 178
Ksp22I TGATCA 1 cut(s) 1000
Kzo9I GATC 6 cut(s) 634, 771, 976, 982, 1000, 1188
LguI GCTCTTC 1 cut(s) 240
LmnI GCTCC 4 cut(s) 4, 37, 435, 615
Lsp1109I GCAGC 3 cut(s) 394, 1154, 1157
LweI GCATC 1 cut(s) 903
MaeI CTAG 5 cut(s) 69, 329, 335, 570, 1053
MaeII ACGT 2 cut(s) 935, 1320
MaeIII GTNAC 4 cut(s) 345, 537, 742, 1321
MalI GATC 6 cut(s) 636, 773, 978, 984, 1002, 1190
MboI GATC 6 cut(s) 634, 771, 976, 982, 1000, 1188
MhlI GDGCHC 4 cut(s) 9, 50, 252, 620
MluCI AATT 8 cut(s) 369, 382, 645, 663, 958, 1112, 1195, 1280
MlyI GAGTC 2 cut(s) 137, 148
MmeI TCCRAC 1 cut(s) 1467
Mph1103I ATGCAT 2 cut(s) 1409, 1420
MseI TTAA 4 cut(s) 150, 1242, 1298, 1495
MslI CAYNNNNRTG 5 cut(s) 683, 839, 860, 984, 1181
MspA1I CMGCKG 1 cut(s) 998
MspCI CTTAAG 1 cut(s) 149
MspI CCGG 1 cut(s) 765
MspR9I CCNGG 1 cut(s) 887
MvaI CCWGG 1 cut(s) 887
MvnI CGCG 1 cut(s) 178
MwoI GCNNNNNNNGC 1 cut(s) 247
NcoI CCATGG 1 cut(s) 917
NdeI CATATG 1 cut(s) 295
NdeII GATC 6 cut(s) 634, 771, 976, 982, 1000, 1188
NheI GCTAGC 1 cut(s) 328
NlaIV GGNNCC 1 cut(s) 39
NmuCI GTSAC 3 cut(s) 345, 537, 742
NsiI ATGCAT 2 cut(s) 1409, 1420
NspI RCATGY 3 cut(s) 799, 947, 1411
OliI CACNNNNGTG 1 cut(s) 839
PaeI GCATGC 1 cut(s) 1411
PagI TCATGA 2 cut(s) 462, 985
PciSI GCTCTTC 1 cut(s) 240
PfeI GAWTC 2 cut(s) 290, 925
PflFI GACNNNGTC 1 cut(s) 1025
PfoI TCCNGGA 1 cut(s) 885
PkrI GCNGC 3 cut(s) 409, 1144, 1147
PleI GAGTC 2 cut(s) 136, 148
PpsI GAGTC 2 cut(s) 136, 148
Psp6I CCWGG 1 cut(s) 885
PspGI CCWGG 1 cut(s) 885
PspN4I GGNNCC 1 cut(s) 39
PspPI GGNCC 2 cut(s) 1205, 1375
PsrI GAACNNNNNNTAC 2 cut(s) 741, 773
PsyI GACNNNGTC 1 cut(s) 1025
RsaI GTAC 5 cut(s) 33, 62, 846, 948, 1253
RsaNI GTAC 5 cut(s) 32, 61, 845, 947, 1252
RseI CAYNNNNRTG 5 cut(s) 683, 839, 860, 984, 1181
SapI GCTCTTC 1 cut(s) 240
SaqAI TTAA 4 cut(s) 150, 1242, 1298, 1495
SatI GCNGC 3 cut(s) 408, 1143, 1146
Sau3AI GATC 6 cut(s) 634, 771, 976, 982, 1000, 1188
Sau96I GGNCC 2 cut(s) 1205, 1375
ScaI AGTACT 2 cut(s) 33, 62
SchI GAGTC 2 cut(s) 137, 148
ScrFI CCNGG 1 cut(s) 887
SduI GDGCHC 4 cut(s) 9, 50, 252, 620
SfaNI GCATC 1 cut(s) 903
SinI GGWCC 1 cut(s) 1205
SmiMI CAYNNNNRTG 5 cut(s) 683, 839, 860, 984, 1181
SmlI CTYRAG 2 cut(s) 132, 149
SmoI CTYRAG 2 cut(s) 132, 149
SphI GCATGC 1 cut(s) 1411
Sse9I AATT 8 cut(s) 369, 382, 645, 663, 958, 1112, 1195, 1280
SsiI CCGC 2 cut(s) 176, 996
SspI AATATT 2 cut(s) 94, 1007
SspMI CTAG 5 cut(s) 69, 329, 335, 570, 1053
StyD4I CCNGG 1 cut(s) 885
StyI CCWWGG 2 cut(s) 778, 917
TaaI ACNGT 3 cut(s) 622, 685, 748
TaiI ACGT 2 cut(s) 938, 1323
TaqI TCGA 2 cut(s) 111, 787
TasI AATT 8 cut(s) 369, 382, 645, 663, 958, 1112, 1195, 1280
TatI WGTACW 3 cut(s) 31, 60, 1251
TfiI GAWTC 2 cut(s) 290, 925
Tru1I TTAA 4 cut(s) 150, 1242, 1298, 1495
Tru9I TTAA 4 cut(s) 150, 1242, 1298, 1495
TscAI CASTG 5 cut(s) 48, 126, 690, 846, 1173
TseFI GTSAC 3 cut(s) 345, 537, 742
TseI GCWGC 3 cut(s) 407, 1142, 1145
Tsp45I GTSAC 3 cut(s) 345, 537, 742
TspDTI ATGAA 5 cut(s) 36, 312, 362, 570, 911
TspGWI ACGGA 2 cut(s) 73, 502
TspRI CASTG 5 cut(s) 48, 126, 690, 846, 1173
Tth111I GACNNNGTC 1 cut(s) 1025
Vha464I CTTAAG 1 cut(s) 149
VpaK11BI GGWCC 1 cut(s) 1205
XapI RAATTY 3 cut(s) 369, 1112, 1195
XbaI TCTAGA 1 cut(s) 569
XceI RCATGY 3 cut(s) 799, 947, 1411
XmiI GTMKAC 2 cut(s) 484, 875
XspI CTAG 5 cut(s) 69, 329, 335, 570, 1053
ZrmI AGTACT 2 cut(s) 33, 62
Zsp2I ATGCAT 2 cut(s) 1409, 1420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.