MD17G1237200.v1.1

O-acyltransferase (WSD1-like)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000634
Physical Location & Seq
Forward (+)
28620938 .. 28626925
5988 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1237200.v1.1.491

Sequence Viewer

Length: 1404 bp
ATGGAGGTTTGGGAAGAGGAACTGGAGCCAGTGAGCCCTAATGGCCACTATTTCAACAGCTCTGTTTTATCGATATCTGTTCTTGGTGTTTTGGAATTTGAAATTCCAATTGAGGACTCTCAAACTTTGTCATTGCTCGAGAATGTGTTCCTGCCCATCAACCCACGCTTCTCCTCTATAATGATTGAAAACAATGGAAAGAAACAATGGAAAAGGGTTGAAGTGAAGCTTGAAGACCATGTTAAAACTCCAATTTTCCCTTCCAATTTGTCGAATGAATCTTATGACGAGTATTTTGATGACTATGTCTCAAGCATAGCTACTAAAAGATTTCCACAAGACAAACCACTGTGGGAAGTTCATGTTATAAAGTACCCAAATAGTAATGCAGCTGGTAATGTAATATTCAAGTTGCACCATGCATTAGGTGATGGCTACTCTCTCATGGGCGCTCTTCTCTCCTGCCTACAAAGGGCTGACAATCCTTCTCTTCCACTAACTTTTCCTTCACTGCTGCCATCAGAAACGAAGAGTGAAAATATTATGACCAAAACTATCTCATCCTTGTTGAGCACCGTATCAGATTTTTGGTGGGGCATTTCAAAGAGCTTCAAGGGGGAAGATGATCCATCACCAATAAAATCCTCGAAAAATGGACTTGAGTTCGCGCCAATTACAATATCAACGATTACGATCTCCCTTGATCGAATCAGATTAATTAAGAGCAAGCTCGGAGTGACGATAAATGGTGTTCTTACTGGGATGGTCTTTCTTGGCTCCCGGCTATATATGCAAGAGATGAACCAAAGCTCAAGGGAAGCGCATAGCACAGCATTGGTTTTACTAAATACAAGGATCATGGCGAATTATACGTCGATTGAAGAAATGGTCAAACCCGATAGCAAGAGTCCATGGGGAAACCATTTTACATTTGTGCATGTTCCCATTCCCAAGTTGACTGAATTCTCAAATGTGCTTGACTACGTTTGGAAGACACATAAAATAATCAAGCAGAAAAGAAATTCTTCAGCTATTTATTTCACTAGTGGGCTATTGAAGATGCTGAACAAGTTTGGAGGCCCCGAGGCAGCATCAAGATACGTCTGTAGCACATTAAAGAACTCGAGCGTGGTGGTCACAAGTATGATTGGCCCGGTGGAAAAAATGTCTTTGGCAAACCAACCAATCAAGGGGTTTTACTTTTTGACAGTTGGGACACCTGAGGAACTTGACATATCAATCGTAAGTTATATGGGAAGGGTGAAGCTTGCCTTCACAGTGAAAAAAGGCATCATAGATCCACAAAAGTTGAAGTTGTGCATGGAAAACGCCCTCAATATGATATCCAACGCCGCAGACAAAAATTCTATGGAAAATAGCACTGAAGAACAGTCACACATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1298 WES_acyltransf

Protein Analysis

468

Amino Acids

52.31

Weight (kDa)

6.58

Isoelectric Point (pI)

45.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WS_DGAT_cat PF03007 68 - 251 1.1e-10 Wax ester synthase/diacylglycerol acyltransferase catalytic domain
WS_DGAT_C PF06974 305 - 448 3.3e-37 WS/DGAT C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000634)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10290 FvH4_3g28230 FvH4_3g28240
malus_domestica MD05G1036400.v1.1 MD10G1159400.v1.1 MD17G1237100.v1.1 MD17G1237200.v1.1 MD17G1237300.v1.1 MD17G1237600.v1.1 MD17G1238200.v1.1
prunus_persica Prupe.3G140400_v2.0.a1 Prupe.3G140700_v2.0.a1 Prupe.3G141100_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G258100_v2.0.a1 Prupe.7G117300_v2.0.a1 Prupe.7G117400_v2.0.a1 Prupe.7G117600_v2.0.a1 Prupe.8G044700_v2.0.a1
pyrus_communis pycom05g02680 pycom17g24220 pycom17g24240
rosa_chinensis RchiOBHm_Chr1g0350141 RchiOBHm_Chr5g0050931 RchiOBHm_Chr5g0051001 RchiOBHm_Chr5g0051021 RchiOBHm_Chr6g0267491
rosa_laevigata RLG00000013999 RLG00000028502 RLG00000034744 RLG00000034748 RLG00000034753
rosa_multiflora Rmu_sc0000446.1_g000056 Rmu_sc0000782.1_g000006 Rmu_sc0002951.1_g000001 Rmu_sc0003674.1_g000026 Rmu_sc0003756.1_g000004 Rmu_sc0011917.1_g000004 Rmu_sc0012005.1_g000019 Rmu_sc0040739.1_g000001
rosa_roxburghii Rroxscaffold_1G00029730 Rroxscaffold_1G00029740 Rroxscaffold_4G00305060 Rroxscaffold_7G00200710
rosa_rugosa Rorug01G0208400 Rorug05G0265200.1 Rorug05G0265300.1 Rorug05G0265400.1 Rorug05G0265500.1 Rorug05G0294000 Rorug06G0033100 Rorug06G0243300
rosa_samantha Rh1BG192100 Rh1BG192200 Rh1DG220600 Rh5AG270300 Rh5AG337200 Rh5AG337700 Rh5BG346700 Rh5BG347400 Rh5CG374300 Rh5DG359700 Rh5DG360500 Rh6AG154500 Rh6BG155100 Rh6BG362300 Rh6CG150300 Rh6DG140300 Rh6DG356300
rosa_wichuraiana Rw0G018820 Rw1G019350 Rw5G025300 Rw5G031770 Rw5G031820 Rw5G031870 Rw5G031910 Rw6G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 368
AccII CGCG 1 cut(s) 668
AciI CCGC 1 cut(s) 1353
AclWI GGATC 3 cut(s) 620, 863, 1292
AcoI YGGCCR 1 cut(s) 43
AcsI RAATTY 5 cut(s) 95, 102, 962, 1021, 1363
AcuI CTGAAG 2 cut(s) 1011, 1404
AfaI GTAC 1 cut(s) 374
AfiI CCNNNNNNNGG 2 cut(s) 472, 1190
AhlI ACTAGT 1 cut(s) 1043
AjuI GAANNNNNNNTTGG 2 cut(s) 244, 276
AluBI AGCT 9 cut(s) 60, 229, 320, 392, 609, 730, 810, 1031, 1267
AluI AGCT 9 cut(s) 60, 229, 320, 392, 609, 730, 810, 1031, 1267
Alw21I GWGCWC 1 cut(s) 575
Alw26I GTCTC 1 cut(s) 313
AlwI GGATC 3 cut(s) 620, 863, 1292
Ama87I CYCGRG 3 cut(s) 137, 1082, 1123
AoxI GGCC 3 cut(s) 43, 1078, 1150
ApeKI GCWGC 3 cut(s) 389, 514, 1088
ApoI RAATTY 5 cut(s) 95, 102, 962, 1021, 1363
ArsI GACNNNNNNTTYG 4 cut(s) 278, 310, 857, 889
AseI ATTAAT 1 cut(s) 716
Asp700I GAANNNNTTC 2 cut(s) 146, 1024
AspLEI GCGC 3 cut(s) 452, 670, 823
AspS9I GGNCC 2 cut(s) 1079, 1151
AsuC2I CCSGG 2 cut(s) 781, 1154
AsuHPI GGTGA 3 cut(s) 440, 624, 1273
AvaI CYCGRG 3 cut(s) 137, 1082, 1123
AxyI CCTNAGG 1 cut(s) 1221
BaeI ACNNNNGTAYC 2 cut(s) 1090, 1123
BalI TGGCCA 1 cut(s) 45
BanII GRGCYC 1 cut(s) 38
BbsI GAAGAC 2 cut(s) 240, 998
Bbv12I GWGCWC 1 cut(s) 575
BbvI GCAGC 3 cut(s) 401, 501, 1100
BccI CCATC 5 cut(s) 164, 425, 526, 637, 757
BcnI CCSGG 2 cut(s) 781, 1154
BcoDI GTCTC 1 cut(s) 313
BcuI ACTAGT 1 cut(s) 1043
BfaI CTAG 1 cut(s) 1044
BfmI CTRYAG 1 cut(s) 1105
BfoI RGCGCY 1 cut(s) 453
BglI GCCNNNNNGGC 1 cut(s) 42
BisI GCNGC 4 cut(s) 390, 515, 1089, 1353
BlsI GCNGC 4 cut(s) 391, 516, 1090, 1354
Bme1390I CCNGG 2 cut(s) 781, 1154
BmeT110I CYCGRG 3 cut(s) 137, 1082, 1123
BmgT120I GGNCC 2 cut(s) 1079, 1151
BmiI GGNNCC 3 cut(s) 27, 778, 1081
BmrFI CCNGG 2 cut(s) 781, 1154
BmrI ACTGGG 1 cut(s) 768
BmsI GCATC 3 cut(s) 1050, 1100, 1299
BmuI ACTGGG 1 cut(s) 768
BpiI GAAGAC 2 cut(s) 240, 998
BpmI CTGGAG 1 cut(s) 44
BpuEI CTTGAG 3 cut(s) 295, 680, 796
BpuMI CCSGG 2 cut(s) 781, 1154
Bsa29I ATCGAT 1 cut(s) 71
BsaBI GATNNNNATC 1 cut(s) 692
BsaJI CCNNGG 2 cut(s) 911, 1083
Bsc4I CCNNNNNNNGG 2 cut(s) 472, 1190
Bse1I ACTGG 3 cut(s) 27, 29, 763
Bse21I CCTNAGG 1 cut(s) 1221
Bse3DI GCAATG 1 cut(s) 131
Bse8I GATNNNNATC 1 cut(s) 692
BseCI ATCGAT 1 cut(s) 71
BseDI CCNNGG 2 cut(s) 911, 1083
BseGI GGATG 2 cut(s) 560, 768
BseJI GATNNNNATC 1 cut(s) 692
BseLI CCNNNNNNNGG 2 cut(s) 472, 1190
BseMI GCAATG 1 cut(s) 131
BseMII CTCAG 1 cut(s) 1212
BseNI ACTGG 3 cut(s) 27, 29, 763
BseRI GAGGAG 1 cut(s) 163
BseXI GCAGC 3 cut(s) 401, 501, 1100
Bsh1236I CGCG 1 cut(s) 668
BshFI GGCC 3 cut(s) 45, 1080, 1152
BshVI ATCGAT 1 cut(s) 71
BsiHKAI GWGCWC 1 cut(s) 575
BsiHKCI CYCGRG 3 cut(s) 137, 1082, 1123
BsiSI CCGG 2 cut(s) 781, 1154
BslFI GGGAC 1 cut(s) 1228
BslI CCNNNNNNNGG 2 cut(s) 472, 1190
BsmAI GTCTC 1 cut(s) 313
BsmFI GGGAC 1 cut(s) 1228
BsnI GGCC 3 cut(s) 45, 1080, 1152
BsoBI CYCGRG 3 cut(s) 137, 1082, 1123
Bsp1286I GDGCHC 2 cut(s) 38, 575
Bsp143I GATC 5 cut(s) 625, 693, 703, 855, 1297
Bsp19I CCATGG 1 cut(s) 911
BspACI CCGC 1 cut(s) 1353
BspANI GGCC 3 cut(s) 45, 1080, 1152
BspCNI CTCAG 1 cut(s) 1213
BspDI ATCGAT 1 cut(s) 71
BspFNI CGCG 1 cut(s) 668
BspLI GGNNCC 3 cut(s) 27, 778, 1081
BspPI GGATC 3 cut(s) 620, 863, 1292
BspQI GCTCTTC 1 cut(s) 459
BsrDI GCAATG 1 cut(s) 131
BsrI ACTGG 3 cut(s) 27, 29, 763
BssECI CCNNGG 2 cut(s) 911, 1083
BssMI GATC 5 cut(s) 625, 693, 703, 855, 1297
BssT1I CCWWGG 1 cut(s) 911
Bst4CI ACNGT 5 cut(s) 351, 577, 1210, 1279, 1392
Bst6I CTCTTC 4 cut(s) 9, 459, 495, 524
BstC8I GCNNGC 2 cut(s) 728, 1269
BstDEI CTNAG 1 cut(s) 1221
BstDSI CCRYGG 1 cut(s) 911
BstF5I GGATG 2 cut(s) 560, 768
BstFNI CGCG 1 cut(s) 668
BstH2I RGCGCY 1 cut(s) 453
BstHHI GCGC 3 cut(s) 452, 670, 823
BstKTI GATC 5 cut(s) 628, 696, 706, 858, 1300
BstMAI GTCTC 1 cut(s) 313
BstMBI GATC 5 cut(s) 625, 693, 703, 855, 1297
BstMWI GCNNNNNNNGC 2 cut(s) 42, 790
BstNSI RCATGY 1 cut(s) 941
BstSCI CCNGG 2 cut(s) 779, 1152
BstSFI CTRYAG 1 cut(s) 1105
BstUI CGCG 1 cut(s) 668
BstV1I GCAGC 3 cut(s) 401, 501, 1100
BstV2I GAAGAC 2 cut(s) 240, 998
BstX2I RGATCY 1 cut(s) 1297
BstYI RGATCY 1 cut(s) 1297
Bsu15I ATCGAT 1 cut(s) 71
Bsu36I CCTNAGG 1 cut(s) 1221
BsuRI GGCC 3 cut(s) 45, 1080, 1152
BsuTUI ATCGAT 1 cut(s) 71
BtgI CCRYGG 1 cut(s) 911
BtsCI GGATG 2 cut(s) 560, 768
BtsI GCAGTG 1 cut(s) 509
BtsIMutI CAGTG 5 cut(s) 36, 347, 509, 1284, 1380
Cac8I GCNNGC 2 cut(s) 728, 1269
CfoI GCGC 3 cut(s) 452, 670, 823
Cfr13I GGNCC 2 cut(s) 1079, 1151
ClaI ATCGAT 1 cut(s) 71
Csp6I GTAC 1 cut(s) 373
CspCI CAANNNNNGTGG 2 cut(s) 1290, 1325
CviAII CATG 8 cut(s) 239, 362, 419, 445, 859, 912, 938, 1321
CviQI GTAC 1 cut(s) 373
DdeI CTNAG 1 cut(s) 1221
DpnI GATC 5 cut(s) 627, 695, 705, 857, 1299
DpnII GATC 5 cut(s) 625, 693, 703, 855, 1297
EaeI YGGCCR 1 cut(s) 43
Eam1104I CTCTTC 4 cut(s) 9, 459, 495, 524
EarI CTCTTC 4 cut(s) 9, 459, 495, 524
Eco130I CCWWGG 1 cut(s) 911
Eco24I GRGCYC 1 cut(s) 38
Eco32I GATATC 2 cut(s) 75, 1344
Eco57I CTGAAG 2 cut(s) 1011, 1404
Eco81I CCTNAGG 1 cut(s) 1221
Eco88I CYCGRG 3 cut(s) 137, 1082, 1123
EcoO109I RGGNCCY 1 cut(s) 1079
EcoRI GAATTC 1 cut(s) 962
EcoRV GATATC 2 cut(s) 75, 1344
EcoT14I CCWWGG 1 cut(s) 911
EcoT22I ATGCAT 1 cut(s) 424
EcoT38I GRGCYC 1 cut(s) 38
ErhI CCWWGG 1 cut(s) 911
FaeI CATG 8 cut(s) 242, 365, 422, 448, 862, 915, 941, 1324
FalI AAGNNNNNCTT 6 cut(s) 213, 245, 1009, 1041, 1256, 1288
FaqI GGGAC 1 cut(s) 1228
FatI CATG 8 cut(s) 238, 361, 418, 444, 858, 911, 937, 1320
Fnu4HI GCNGC 4 cut(s) 390, 515, 1089, 1353
FokI GGATG 2 cut(s) 547, 775
FriOI GRGCYC 1 cut(s) 38
Fsp4HI GCNGC 4 cut(s) 390, 515, 1089, 1353
FspBI CTAG 1 cut(s) 1044
GlaI GCGC 3 cut(s) 451, 669, 822
GluI GCNGC 4 cut(s) 390, 515, 1089, 1353
GsuI CTGGAG 1 cut(s) 44
HaeII RGCGCY 1 cut(s) 453
HaeIII GGCC 3 cut(s) 45, 1080, 1152
HapII CCGG 2 cut(s) 781, 1154
HhaI GCGC 3 cut(s) 452, 670, 823
Hin1II CATG 8 cut(s) 242, 365, 422, 448, 862, 915, 941, 1324
Hin6I GCGC 3 cut(s) 450, 668, 821
HinP1I GCGC 3 cut(s) 450, 668, 821
HincII GTYRAC 1 cut(s) 957
HindII GTYRAC 1 cut(s) 957
HindIII AAGCTT 2 cut(s) 227, 1265
HinfI GANTC 4 cut(s) 116, 278, 708, 907
HpaII CCGG 2 cut(s) 781, 1154
HphI GGTGA 3 cut(s) 440, 624, 1273
Hpy166II GTNNAC 1 cut(s) 957
Hpy188I TCNGA 4 cut(s) 523, 583, 713, 734
Hpy188III TCNNGA 2 cut(s) 139, 1095
Hpy8I GTNNAC 1 cut(s) 957
Hpy99I CGWCG 1 cut(s) 877
HpyAV CCTTC 5 cut(s) 270, 495, 516, 1251, 1282
HpyCH4III ACNGT 5 cut(s) 351, 577, 1210, 1279, 1392
HpyCH4IV ACGT 3 cut(s) 872, 984, 1101
HpyCH4V TGCA 6 cut(s) 389, 415, 422, 793, 937, 1320
HpyF10VI GCNNNNNNNGC 2 cut(s) 42, 790
HpyF3I CTNAG 1 cut(s) 1221
HpySE526I ACGT 3 cut(s) 872, 984, 1101
Hsp92II CATG 8 cut(s) 242, 365, 422, 448, 862, 915, 941, 1324
HspAI GCGC 3 cut(s) 450, 668, 821
Kzo9I GATC 5 cut(s) 625, 693, 703, 855, 1297
LguI GCTCTTC 1 cut(s) 459
LmnI GCTCC 2 cut(s) 25, 782
LpnPI CCDG 9 cut(s) 8, 42, 164, 378, 475, 744, 794, 1167, 1233
Lsp1109I GCAGC 3 cut(s) 401, 501, 1100
LweI GCATC 3 cut(s) 1050, 1100, 1299
MaeI CTAG 1 cut(s) 1044
MaeII ACGT 3 cut(s) 872, 984, 1101
MaeIII GTNAC 3 cut(s) 736, 1135, 1392
MalI GATC 5 cut(s) 627, 695, 705, 857, 1299
MboI GATC 5 cut(s) 625, 693, 703, 855, 1297
MfeI CAATTG 1 cut(s) 108
MflI RGATCY 1 cut(s) 1297
MhlI GDGCHC 2 cut(s) 38, 575
MlsI TGGCCA 1 cut(s) 45
MluNI TGGCCA 1 cut(s) 45
MlyI GAGTC 2 cut(s) 110, 916
MmeI TCCRAC 1 cut(s) 1371
MnlI CCTC 8 cut(s) 10, 106, 184, 655, 1070, 1078, 1216, 1343
Mox20I TGGCCA 1 cut(s) 45
Mph1103I ATGCAT 1 cut(s) 424
MroXI GAANNNNTTC 2 cut(s) 146, 1024
MscI TGGCCA 1 cut(s) 45
MseI TTAA 4 cut(s) 243, 716, 720, 1115
MslI CAYNNNNRTG 1 cut(s) 1142
Msp20I TGGCCA 1 cut(s) 45
MspA1I CMGCKG 1 cut(s) 392
MspI CCGG 2 cut(s) 781, 1154
MspR9I CCNGG 2 cut(s) 781, 1154
MunI CAATTG 1 cut(s) 108
MvnI CGCG 1 cut(s) 668
MwoI GCNNNNNNNGC 2 cut(s) 42, 790
NciI CCSGG 2 cut(s) 781, 1154
NcoI CCATGG 1 cut(s) 911
NdeII GATC 5 cut(s) 625, 693, 703, 855, 1297
NlaIII CATG 8 cut(s) 242, 365, 422, 448, 862, 915, 941, 1324
NlaIV GGNNCC 3 cut(s) 27, 778, 1081
NmuCI GTSAC 3 cut(s) 736, 1135, 1392
NsiI ATGCAT 1 cut(s) 424
NspI RCATGY 1 cut(s) 941
PacI TTAATTAA 1 cut(s) 720
PaeR7I CTCGAG 2 cut(s) 137, 1123
PciSI GCTCTTC 1 cut(s) 459
PdmI GAANNNNTTC 2 cut(s) 146, 1024
PfeI GAWTC 2 cut(s) 278, 708
PflFI GACNNNGTC 1 cut(s) 305
PkrI GCNGC 4 cut(s) 391, 516, 1090, 1354
PleI GAGTC 2 cut(s) 110, 915
PpsI GAGTC 2 cut(s) 110, 915
PshBI ATTAAT 1 cut(s) 716
PsiI TTATAA 1 cut(s) 368
PspN4I GGNNCC 3 cut(s) 27, 778, 1081
PspPI GGNCC 2 cut(s) 1079, 1151
PspXI VCTCGAGB 1 cut(s) 1123
PsuI RGATCY 1 cut(s) 1297
PsyI GACNNNGTC 1 cut(s) 305
PvuII CAGCTG 1 cut(s) 392
RsaI GTAC 1 cut(s) 374
RsaNI GTAC 1 cut(s) 373
RseI CAYNNNNRTG 1 cut(s) 1142
SapI GCTCTTC 1 cut(s) 459
SaqAI TTAA 4 cut(s) 243, 716, 720, 1115
SatI GCNGC 4 cut(s) 390, 515, 1089, 1353
Sau3AI GATC 5 cut(s) 625, 693, 703, 855, 1297
Sau96I GGNCC 2 cut(s) 1079, 1151
SchI GAGTC 2 cut(s) 110, 916
ScrFI CCNGG 2 cut(s) 781, 1154
SduI GDGCHC 2 cut(s) 38, 575
SfaNI GCATC 3 cut(s) 1050, 1100, 1299
SfcI CTRYAG 1 cut(s) 1105
Sfr274I CTCGAG 2 cut(s) 137, 1123
SlaI CTCGAG 2 cut(s) 137, 1123
SmiMI CAYNNNNRTG 1 cut(s) 1142
SmlI CTYRAG 5 cut(s) 137, 310, 659, 811, 1123
SmoI CTYRAG 5 cut(s) 137, 310, 659, 811, 1123
SpeI ACTAGT 1 cut(s) 1043
SsiI CCGC 1 cut(s) 1353
SspI AATATT 2 cut(s) 405, 541
SspMI CTAG 1 cut(s) 1044
StyD4I CCNGG 2 cut(s) 779, 1152
StyI CCWWGG 1 cut(s) 911
TaaI ACNGT 5 cut(s) 351, 577, 1210, 1279, 1392
TaiI ACGT 3 cut(s) 875, 987, 1104
TaqI TCGA 7 cut(s) 71, 138, 272, 647, 706, 875, 1124
TauI GCSGC 1 cut(s) 1355
TfiI GAWTC 2 cut(s) 278, 708
Tru1I TTAA 4 cut(s) 243, 716, 720, 1115
Tru9I TTAA 4 cut(s) 243, 716, 720, 1115
TscAI CASTG 5 cut(s) 36, 354, 516, 1284, 1387
TseFI GTSAC 3 cut(s) 736, 1135, 1392
TseI GCWGC 3 cut(s) 389, 514, 1088
Tsp45I GTSAC 3 cut(s) 736, 1135, 1392
TspDTI ATGAA 3 cut(s) 291, 350, 815
TspRI CASTG 5 cut(s) 36, 354, 516, 1284, 1387
Tth111I GACNNNGTC 1 cut(s) 305
VspI ATTAAT 1 cut(s) 716
XapI RAATTY 5 cut(s) 95, 102, 962, 1021, 1363
XceI RCATGY 1 cut(s) 941
XhoI CTCGAG 2 cut(s) 137, 1123
XmnI GAANNNNTTC 2 cut(s) 146, 1024
XspI CTAG 1 cut(s) 1044
Zsp2I ATGCAT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.