MD17G1237600.v1.1

O-acyltransferase (WSD1-like)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000634
Physical Location & Seq
Forward (+)
28689260 .. 28694091
4832 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1237600.v1.1.491

Sequence Viewer

Length: 1299 bp
ATGGAGGTTTGGGAAGAGGAACTGGAGCCAGTGAGCCCTAATGGCCACTATTTCAACAGCTCTGTTTTATCGGTATCAGTTCTTGCTGTTTTGGAATATGAAATTCCAATTGATGACTCTCAAACTTTGTCATTGCTCGAGAATGTGTTCCTGCCCATCAGCCCACGCTTCTCCTCTATAATGATTGAAAAAAATGGAAAGAAACAATGGAAAAAGGTTGAAGTGAAACTTGAAGACCATGTTAAAACTCCAATCTTCCCATCCAATATGTCGAATGAATCATATGACGAGTATTTTGATGACTATATCTCAAGCATAGCTACAAAAAGATTTCCACAAGACAAACCACTGTGGGAAGTTCATGTTATAAAGTACCCAAATAGCAATGCAGCTGGTAATGTAATATTCAAGTTGCACCATGCATTAGGTGATGGCTACTCTCTCATGGGCGCTCTTCTCTCCTGTCTACAAAGGGCTGACAATCCTTCTCTTCCACTAACTTTTCCTTCACGGCTGCCATCAGAAACGAAGAGTGAAAATGTTGTGACCAAAACTATCTCATCCTTGTTGAGCACCATATCCGATTTTTGGTGGGGCATTTCAAAGAGCTTCAGGGGAGAAGATGATCCATCACCAATAAAATCCTCGAATCATGGACTTGAGTTCGCGCCAATTACAATATCAACGATTACGATCTCCCTTGATCGAATCAAATTAATTAAGAGCAGGCTCGGAGTGACGATAAATGACATTCTTACTGGGATGGTCTTTCTTGGCTCTCGGCTATATATGCAAGAGATGAACCAAAGCTCAAGGGAAGTGCATAGCACAGCATTAGTTTTACTAAATACAAGGTTCATGGCGAATTATGCGTCGACTGAAGAAATGATCAAACCCAACAGCAAGAGCCCATGGGGAAATCGTTTTGCATTCTTGCATGTTCCCATTCCCAAGTTGACTGAATTCTCAGATGTGCTTGACTTCGTGTGGAATGCACAAAAAATAATCAAGCAGAAAAGAAGTTCTTTAGCTATTTATTTCACTAGTGGGCTATTGGAGATGCTGAACAAGTTTGGAGGCCACGAGGCAGCATCAAGATACGTCCATAGCACATTAAAGAACTCGAGCATGATGATCTCAAGTATGATTGGCCCGGTGCAAAAAATGTCTTTGGCAAACCAACCAATCAAAGGGTTGTACTTTTTGGTAGTTGGGACACCTGAGGTAAACCGAATAATTTTTTTGACAAAGTTTAAAAAACTAAAGGAAATGAAAGTTAATTATTGGTTTATTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1298 WES_acyltransf

Protein Analysis

433

Amino Acids

48.96

Weight (kDa)

8.61

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WS_DGAT_cat PF03007 68 - 251 1.3e-11 Wax ester synthase/diacylglycerol acyltransferase catalytic domain
WS_DGAT_C PF06974 305 - 410 9e-26 WS/DGAT C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000634)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10290 FvH4_3g28230 FvH4_3g28240
malus_domestica MD05G1036400.v1.1 MD10G1159400.v1.1 MD17G1237100.v1.1 MD17G1237200.v1.1 MD17G1237300.v1.1 MD17G1237600.v1.1 MD17G1238200.v1.1
prunus_persica Prupe.3G140400_v2.0.a1 Prupe.3G140700_v2.0.a1 Prupe.3G141100_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G258100_v2.0.a1 Prupe.7G117300_v2.0.a1 Prupe.7G117400_v2.0.a1 Prupe.7G117600_v2.0.a1 Prupe.8G044700_v2.0.a1
pyrus_communis pycom05g02680 pycom17g24220 pycom17g24240
rosa_chinensis RchiOBHm_Chr1g0350141 RchiOBHm_Chr5g0050931 RchiOBHm_Chr5g0051001 RchiOBHm_Chr5g0051021 RchiOBHm_Chr6g0267491
rosa_laevigata RLG00000013999 RLG00000028502 RLG00000034744 RLG00000034748 RLG00000034753
rosa_multiflora Rmu_sc0000446.1_g000056 Rmu_sc0000782.1_g000006 Rmu_sc0002951.1_g000001 Rmu_sc0003674.1_g000026 Rmu_sc0003756.1_g000004 Rmu_sc0011917.1_g000004 Rmu_sc0012005.1_g000019 Rmu_sc0040739.1_g000001
rosa_roxburghii Rroxscaffold_1G00029730 Rroxscaffold_1G00029740 Rroxscaffold_4G00305060 Rroxscaffold_7G00200710
rosa_rugosa Rorug01G0208400 Rorug05G0265200.1 Rorug05G0265300.1 Rorug05G0265400.1 Rorug05G0265500.1 Rorug05G0294000 Rorug06G0033100 Rorug06G0243300
rosa_samantha Rh1BG192100 Rh1BG192200 Rh1DG220600 Rh5AG270300 Rh5AG337200 Rh5AG337700 Rh5BG346700 Rh5BG347400 Rh5CG374300 Rh5DG359700 Rh5DG360500 Rh6AG154500 Rh6BG155100 Rh6BG362300 Rh6CG150300 Rh6DG140300 Rh6DG356300
rosa_wichuraiana Rw0G018820 Rw1G019350 Rw5G025300 Rw5G031770 Rw5G031820 Rw5G031870 Rw5G031910 Rw6G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 368
AccI GTMKAC 2 cut(s) 466, 875
AccII CGCG 1 cut(s) 668
AclWI GGATC 1 cut(s) 620
AcoI YGGCCR 1 cut(s) 43
AcsI RAATTY 2 cut(s) 102, 962
AcuI CTGAAG 2 cut(s) 595, 900
AfaI GTAC 2 cut(s) 374, 1199
AfiI CCNNNNNNNGG 2 cut(s) 588, 1190
AgsI TTSAA 6 cut(s) 55, 188, 221, 233, 409, 603
AhlI ACTAGT 1 cut(s) 1043
AluBI AGCT 6 cut(s) 60, 320, 392, 609, 810, 1031
AluI AGCT 6 cut(s) 60, 320, 392, 609, 810, 1031
Alw21I GWGCWC 1 cut(s) 575
AlwI GGATC 1 cut(s) 620
Ama87I CYCGRG 2 cut(s) 137, 1123
AoxI GGCC 3 cut(s) 43, 1078, 1150
ApeKI GCWGC 3 cut(s) 389, 514, 1088
ApoI RAATTY 2 cut(s) 102, 962
ArsI GACNNNNNNTTYG 4 cut(s) 278, 310, 857, 889
AseI ATTAAT 1 cut(s) 716
Asp700I GAANNNNTTC 1 cut(s) 146
AspLEI GCGC 2 cut(s) 452, 670
AspS9I GGNCC 1 cut(s) 1151
AsuC2I CCSGG 1 cut(s) 1154
AsuHPI GGTGA 2 cut(s) 440, 624
AvaI CYCGRG 2 cut(s) 137, 1123
AxyI CCTNAGG 1 cut(s) 1221
BalI TGGCCA 1 cut(s) 45
BanII GRGCYC 2 cut(s) 38, 911
BauI CACGAG 1 cut(s) 1082
BbsI GAAGAC 1 cut(s) 240
Bbv12I GWGCWC 1 cut(s) 575
BbvI GCAGC 3 cut(s) 401, 501, 1100
BccI CCATC 6 cut(s) 164, 268, 425, 526, 637, 757
BceAI ACGGC 1 cut(s) 527
BclI TGATCA 1 cut(s) 888
BcnI CCSGG 1 cut(s) 1154
BcuI ACTAGT 1 cut(s) 1043
BfaI CTAG 1 cut(s) 1044
BfoI RGCGCY 1 cut(s) 453
BglI GCCNNNNNGGC 1 cut(s) 42
BisI GCNGC 3 cut(s) 390, 515, 1089
BlsI GCNGC 3 cut(s) 391, 516, 1090
Bme1390I CCNGG 1 cut(s) 1154
BmeT110I CYCGRG 2 cut(s) 137, 1123
BmgT120I GGNCC 1 cut(s) 1151
BmiI GGNNCC 1 cut(s) 27
BmrFI CCNGG 1 cut(s) 1154
BmrI ACTGGG 1 cut(s) 768
BmsI GCATC 2 cut(s) 1050, 1100
BmuI ACTGGG 1 cut(s) 768
BpiI GAAGAC 1 cut(s) 240
BpmI CTGGAG 1 cut(s) 44
BpuEI CTTGAG 4 cut(s) 295, 680, 796, 1123
BpuMI CCSGG 1 cut(s) 1154
BsaBI GATNNNNATC 1 cut(s) 692
BsaJI CCNNGG 1 cut(s) 911
Bsc4I CCNNNNNNNGG 2 cut(s) 588, 1190
Bse1I ACTGG 3 cut(s) 27, 29, 763
Bse21I CCTNAGG 1 cut(s) 1221
Bse3DI GCAATG 2 cut(s) 131, 391
Bse8I GATNNNNATC 1 cut(s) 692
BseDI CCNNGG 1 cut(s) 911
BseGI GGATG 3 cut(s) 260, 560, 768
BseJI GATNNNNATC 1 cut(s) 692
BseLI CCNNNNNNNGG 2 cut(s) 588, 1190
BseMI GCAATG 2 cut(s) 131, 391
BseMII CTCAG 2 cut(s) 981, 1212
BseNI ACTGG 3 cut(s) 27, 29, 763
BseRI GAGGAG 1 cut(s) 163
BseXI GCAGC 3 cut(s) 401, 501, 1100
Bsh1236I CGCG 1 cut(s) 668
BshFI GGCC 3 cut(s) 45, 1080, 1152
BsiHKAI GWGCWC 1 cut(s) 575
BsiHKCI CYCGRG 2 cut(s) 137, 1123
BsiSI CCGG 1 cut(s) 1154
BslFI GGGAC 1 cut(s) 1228
BslI CCNNNNNNNGG 2 cut(s) 588, 1190
BsmFI GGGAC 1 cut(s) 1228
BsmI GAATGC 2 cut(s) 929, 997
BsnI GGCC 3 cut(s) 45, 1080, 1152
BsoBI CYCGRG 2 cut(s) 137, 1123
Bsp1286I GDGCHC 3 cut(s) 38, 575, 911
Bsp143I GATC 5 cut(s) 625, 693, 703, 888, 1134
Bsp19I CCATGG 1 cut(s) 911
BspANI GGCC 3 cut(s) 45, 1080, 1152
BspCNI CTCAG 2 cut(s) 980, 1213
BspFNI CGCG 1 cut(s) 668
BspLI GGNNCC 1 cut(s) 27
BspPI GGATC 1 cut(s) 620
BspQI GCTCTTC 1 cut(s) 459
BsrDI GCAATG 2 cut(s) 131, 391
BsrI ACTGG 3 cut(s) 27, 29, 763
BssECI CCNNGG 1 cut(s) 911
BssMI GATC 5 cut(s) 625, 693, 703, 888, 1134
BssSI CACGAG 1 cut(s) 1082
BssT1I CCWWGG 1 cut(s) 911
Bst2BI CACGAG 1 cut(s) 1082
Bst4CI ACNGT 1 cut(s) 351
Bst6I CTCTTC 4 cut(s) 9, 459, 495, 524
BstC8I GCNNGC 1 cut(s) 728
BstDEI CTNAG 2 cut(s) 967, 1221
BstDSI CCRYGG 1 cut(s) 911
BstF5I GGATG 3 cut(s) 260, 560, 768
BstFNI CGCG 1 cut(s) 668
BstH2I RGCGCY 1 cut(s) 453
BstHHI GCGC 2 cut(s) 452, 670
BstKTI GATC 5 cut(s) 628, 696, 706, 891, 1137
BstMBI GATC 5 cut(s) 625, 693, 703, 888, 1134
BstMWI GCNNNNNNNGC 3 cut(s) 42, 790, 869
BstNSI RCATGY 1 cut(s) 941
BstSCI CCNGG 1 cut(s) 1152
BstUI CGCG 1 cut(s) 668
BstV1I GCAGC 3 cut(s) 401, 501, 1100
BstV2I GAAGAC 1 cut(s) 240
Bsu36I CCTNAGG 1 cut(s) 1221
BsuRI GGCC 3 cut(s) 45, 1080, 1152
BtgI CCRYGG 1 cut(s) 911
BtsCI GGATG 3 cut(s) 260, 560, 768
BtsIMutI CAGTG 2 cut(s) 36, 347
Cac8I GCNNGC 1 cut(s) 728
CfoI GCGC 2 cut(s) 452, 670
Cfr13I GGNCC 1 cut(s) 1151
CseI GACGC 1 cut(s) 861
Csp6I GTAC 2 cut(s) 373, 1198
CviAII CATG 9 cut(s) 239, 362, 419, 445, 653, 859, 912, 938, 1129
CviQI GTAC 2 cut(s) 373, 1198
DdeI CTNAG 2 cut(s) 967, 1221
DpnI GATC 5 cut(s) 627, 695, 705, 890, 1136
DpnII GATC 5 cut(s) 625, 693, 703, 888, 1134
DraI TTTAAA 1 cut(s) 1255
EaeI YGGCCR 1 cut(s) 43
Eam1104I CTCTTC 4 cut(s) 9, 459, 495, 524
EarI CTCTTC 4 cut(s) 9, 459, 495, 524
Eco130I CCWWGG 1 cut(s) 911
Eco24I GRGCYC 2 cut(s) 38, 911
Eco57I CTGAAG 2 cut(s) 595, 900
Eco81I CCTNAGG 1 cut(s) 1221
Eco88I CYCGRG 2 cut(s) 137, 1123
EcoRI GAATTC 1 cut(s) 962
EcoT14I CCWWGG 1 cut(s) 911
EcoT22I ATGCAT 1 cut(s) 424
EcoT38I GRGCYC 2 cut(s) 38, 911
ErhI CCWWGG 1 cut(s) 911
FaeI CATG 9 cut(s) 242, 365, 422, 448, 656, 862, 915, 941, 1132
FalI AAGNNNNNCTT 4 cut(s) 213, 245, 1009, 1041
FaqI GGGAC 1 cut(s) 1228
FatI CATG 9 cut(s) 238, 361, 418, 444, 652, 858, 911, 937, 1128
FauNDI CATATG 1 cut(s) 283
FbaI TGATCA 1 cut(s) 888
FblI GTMKAC 2 cut(s) 466, 875
Fnu4HI GCNGC 3 cut(s) 390, 515, 1089
FokI GGATG 3 cut(s) 247, 547, 775
FriOI GRGCYC 2 cut(s) 38, 911
Fsp4HI GCNGC 3 cut(s) 390, 515, 1089
FspBI CTAG 1 cut(s) 1044
GlaI GCGC 2 cut(s) 451, 669
GluI GCNGC 3 cut(s) 390, 515, 1089
GsuI CTGGAG 1 cut(s) 44
HaeII RGCGCY 1 cut(s) 453
HaeIII GGCC 3 cut(s) 45, 1080, 1152
HapII CCGG 1 cut(s) 1154
HgaI GACGC 1 cut(s) 861
HhaI GCGC 2 cut(s) 452, 670
Hin1II CATG 9 cut(s) 242, 365, 422, 448, 656, 862, 915, 941, 1132
Hin6I GCGC 2 cut(s) 450, 668
HinP1I GCGC 2 cut(s) 450, 668
HincII GTYRAC 2 cut(s) 876, 957
HindII GTYRAC 2 cut(s) 876, 957
HinfI GANTC 4 cut(s) 116, 278, 649, 708
HpaII CCGG 1 cut(s) 1154
HphI GGTGA 2 cut(s) 440, 624
Hpy166II GTNNAC 4 cut(s) 467, 876, 957, 1228
Hpy188I TCNGA 4 cut(s) 523, 583, 734, 970
Hpy188III TCNNGA 2 cut(s) 139, 1095
Hpy8I GTNNAC 4 cut(s) 467, 876, 957, 1228
Hpy99I CGWCG 1 cut(s) 877
HpyAV CCTTC 2 cut(s) 495, 516
HpyCH4III ACNGT 1 cut(s) 351
HpyCH4IV ACGT 1 cut(s) 1101
HpyCH4V TGCA 9 cut(s) 389, 415, 422, 793, 823, 929, 937, 995, 1159
HpyF10VI GCNNNNNNNGC 3 cut(s) 42, 790, 869
HpyF3I CTNAG 2 cut(s) 967, 1221
HpySE526I ACGT 1 cut(s) 1101
Hsp92II CATG 9 cut(s) 242, 365, 422, 448, 656, 862, 915, 941, 1132
HspAI GCGC 2 cut(s) 450, 668
Ksp22I TGATCA 1 cut(s) 888
Kzo9I GATC 5 cut(s) 625, 693, 703, 888, 1134
LguI GCTCTTC 1 cut(s) 459
LmnI GCTCC 1 cut(s) 25
Lsp1109I GCAGC 3 cut(s) 401, 501, 1100
LweI GCATC 2 cut(s) 1050, 1100
MaeI CTAG 1 cut(s) 1044
MaeII ACGT 1 cut(s) 1101
MaeIII GTNAC 2 cut(s) 544, 736
MalI GATC 5 cut(s) 627, 695, 705, 890, 1136
MboI GATC 5 cut(s) 625, 693, 703, 888, 1134
MboII GAAGA 8 cut(s) 26, 245, 247, 446, 482, 541, 632, 893
MfeI CAATTG 1 cut(s) 108
MhlI GDGCHC 3 cut(s) 38, 575, 911
MlsI TGGCCA 1 cut(s) 45
MluCI AATT 9 cut(s) 102, 108, 672, 713, 717, 865, 962, 1236, 1279
MluNI TGGCCA 1 cut(s) 45
MlyI GAGTC 1 cut(s) 110
MnlI CCTC 6 cut(s) 10, 184, 655, 1070, 1078, 1216
Mox20I TGGCCA 1 cut(s) 45
Mph1103I ATGCAT 1 cut(s) 424
MroXI GAANNNNTTC 1 cut(s) 146
MscI TGGCCA 1 cut(s) 45
MseI TTAA 7 cut(s) 243, 716, 720, 1115, 1254, 1278, 1297
Msp20I TGGCCA 1 cut(s) 45
MspA1I CMGCKG 1 cut(s) 392
MspI CCGG 1 cut(s) 1154
MspR9I CCNGG 1 cut(s) 1154
MunI CAATTG 1 cut(s) 108
Mva1269I GAATGC 2 cut(s) 929, 997
MvnI CGCG 1 cut(s) 668
MwoI GCNNNNNNNGC 3 cut(s) 42, 790, 869
NciI CCSGG 1 cut(s) 1154
NcoI CCATGG 1 cut(s) 911
NdeI CATATG 1 cut(s) 283
NdeII GATC 5 cut(s) 625, 693, 703, 888, 1134
NlaIII CATG 9 cut(s) 242, 365, 422, 448, 656, 862, 915, 941, 1132
NlaIV GGNNCC 1 cut(s) 27
NmeAIII GCCGAG 1 cut(s) 760
NmuCI GTSAC 2 cut(s) 544, 736
NsiI ATGCAT 1 cut(s) 424
NspI RCATGY 1 cut(s) 941
PacI TTAATTAA 1 cut(s) 720
PaeR7I CTCGAG 2 cut(s) 137, 1123
PciSI GCTCTTC 1 cut(s) 459
PctI GAATGC 2 cut(s) 929, 997
PdmI GAANNNNTTC 1 cut(s) 146
PfeI GAWTC 3 cut(s) 278, 649, 708
PkrI GCNGC 3 cut(s) 391, 516, 1090
PleI GAGTC 1 cut(s) 110
PpsI GAGTC 1 cut(s) 110
PshBI ATTAAT 1 cut(s) 716
PsiI TTATAA 1 cut(s) 368
PspN4I GGNNCC 1 cut(s) 27
PspPI GGNCC 1 cut(s) 1151
PspXI VCTCGAGB 1 cut(s) 1123
PvuII CAGCTG 1 cut(s) 392
RsaI GTAC 2 cut(s) 374, 1199
RsaNI GTAC 2 cut(s) 373, 1198
SalI GTCGAC 1 cut(s) 874
SapI GCTCTTC 1 cut(s) 459
SaqAI TTAA 7 cut(s) 243, 716, 720, 1115, 1254, 1278, 1297
SatI GCNGC 3 cut(s) 390, 515, 1089
Sau3AI GATC 5 cut(s) 625, 693, 703, 888, 1134
Sau96I GGNCC 1 cut(s) 1151
SchI GAGTC 1 cut(s) 110
ScrFI CCNGG 1 cut(s) 1154
SduI GDGCHC 3 cut(s) 38, 575, 911
SfaNI GCATC 2 cut(s) 1050, 1100
Sfr274I CTCGAG 2 cut(s) 137, 1123
SlaI CTCGAG 2 cut(s) 137, 1123
SmlI CTYRAG 6 cut(s) 137, 310, 659, 811, 1123, 1138
SmoI CTYRAG 6 cut(s) 137, 310, 659, 811, 1123, 1138
SpeI ACTAGT 1 cut(s) 1043
Sse9I AATT 9 cut(s) 102, 108, 672, 713, 717, 865, 962, 1236, 1279
SspI AATATT 1 cut(s) 405
SspMI CTAG 1 cut(s) 1044
StyD4I CCNGG 1 cut(s) 1152
StyI CCWWGG 1 cut(s) 911
TaaI ACNGT 1 cut(s) 351
TaiI ACGT 1 cut(s) 1104
TaqI TCGA 6 cut(s) 138, 272, 647, 706, 875, 1124
TasI AATT 9 cut(s) 102, 108, 672, 713, 717, 865, 962, 1236, 1279
TatI WGTACW 1 cut(s) 1197
TfiI GAWTC 3 cut(s) 278, 649, 708
Tru1I TTAA 7 cut(s) 243, 716, 720, 1115, 1254, 1278, 1297
Tru9I TTAA 7 cut(s) 243, 716, 720, 1115, 1254, 1278, 1297
TscAI CASTG 2 cut(s) 36, 354
TseFI GTSAC 2 cut(s) 544, 736
TseI GCWGC 3 cut(s) 389, 514, 1088
Tsp45I GTSAC 2 cut(s) 544, 736
TspDTI ATGAA 6 cut(s) 114, 291, 350, 815, 847, 1286
TspRI CASTG 2 cut(s) 36, 354
VspI ATTAAT 1 cut(s) 716
XapI RAATTY 2 cut(s) 102, 962
XceI RCATGY 1 cut(s) 941
XhoI CTCGAG 2 cut(s) 137, 1123
XmiI GTMKAC 2 cut(s) 466, 875
XmnI GAANNNNTTC 1 cut(s) 146
XspI CTAG 1 cut(s) 1044
Zsp2I ATGCAT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.