Wax ester synthase-like Acyl-CoA acyltransferase domain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 1065 |
| AccB1I | GGYRCC | 1 cut(s) | 1065 |
| AccI | GTMKAC | 3 cut(s) | 117, 713, 886 |
| AciI | CCGC | 2 cut(s) | 864, 896 |
| AclWI | GGATC | 2 cut(s) | 418, 549 |
| AcsI | RAATTY | 3 cut(s) | 1100, 1224, 1391 |
| AfaI | GTAC | 3 cut(s) | 386, 818, 1067 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 1220, 1368 |
| AgeI | ACCGGT | 1 cut(s) | 1241 |
| AleI | CACNNNNGTG | 1 cut(s) | 1252 |
| AluBI | AGCT | 9 cut(s) | 66, 241, 404, 545, 724, 760, 1291, 1310, 1382 |
| AluI | AGCT | 9 cut(s) | 66, 241, 404, 545, 724, 760, 1291, 1310, 1382 |
| Alw21I | GWGCWC | 2 cut(s) | 466, 1249 |
| Alw26I | GTCTC | 3 cut(s) | 482, 976, 1036 |
| Alw44I | GTGCAC | 1 cut(s) | 1245 |
| AlwI | GGATC | 2 cut(s) | 418, 549 |
| Ama87I | CYCGRG | 1 cut(s) | 43 |
| AoxI | GGCC | 2 cut(s) | 356, 1108 |
| ApaLI | GTGCAC | 1 cut(s) | 1245 |
| ApeKI | GCWGC | 4 cut(s) | 401, 724, 893, 1382 |
| ApoI | RAATTY | 3 cut(s) | 1100, 1224, 1391 |
| AseI | ATTAAT | 1 cut(s) | 165 |
| AsiGI | ACCGGT | 1 cut(s) | 1241 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1054 |
| Asp718I | GGTACC | 1 cut(s) | 1065 |
| AspS9I | GGNCC | 3 cut(s) | 672, 1109, 1181 |
| AsuC2I | CCSGG | 2 cut(s) | 44, 45 |
| AsuHPI | GGTGA | 3 cut(s) | 49, 1063, 1202 |
| AvaI | CYCGRG | 1 cut(s) | 43 |
| AvaII | GGWCC | 2 cut(s) | 672, 1181 |
| AxyI | CCTNAGG | 1 cut(s) | 1113 |
| BaeGI | GKGCMC | 2 cut(s) | 981, 1249 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1320, 1353 |
| BanI | GGYRCC | 1 cut(s) | 1065 |
| BanII | GRGCYC | 1 cut(s) | 44 |
| BarI | GAAGNNNNNNTAC | 1 cut(s) | 38 |
| BbsI | GAAGAC | 2 cut(s) | 145, 252 |
| Bbv12I | GWGCWC | 2 cut(s) | 466, 1249 |
| BbvI | GCAGC | 4 cut(s) | 413, 711, 880, 1369 |
| BccI | CCATC | 4 cut(s) | 191, 437, 677, 823 |
| BceAI | ACGGC | 2 cut(s) | 77, 578 |
| BcnI | CCSGG | 2 cut(s) | 44, 45 |
| BcoDI | GTCTC | 3 cut(s) | 482, 976, 1036 |
| BfaI | CTAG | 1 cut(s) | 1077 |
| BisI | GCNGC | 4 cut(s) | 402, 725, 894, 1383 |
| BlsI | GCNGC | 4 cut(s) | 403, 726, 895, 1384 |
| Bme1390I | CCNGG | 2 cut(s) | 44, 45 |
| Bme18I | GGWCC | 2 cut(s) | 672, 1181 |
| BmeT110I | CYCGRG | 1 cut(s) | 43 |
| BmgT120I | GGNCC | 3 cut(s) | 672, 1109, 1181 |
| BmiI | GGNNCC | 1 cut(s) | 1067 |
| BmrFI | CCNGG | 2 cut(s) | 44, 45 |
| BmsI | GCATC | 3 cut(s) | 341, 816, 1348 |
| BoxI | GACNNNNGTC | 1 cut(s) | 703 |
| BpiI | GAAGAC | 2 cut(s) | 145, 252 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1292 |
| BpuEI | CTTGAG | 1 cut(s) | 1138 |
| BpuMI | CCSGG | 2 cut(s) | 44, 45 |
| BsaBI | GATNNNNATC | 1 cut(s) | 923 |
| BsaJI | CCNNGG | 2 cut(s) | 43, 436 |
| BsaWI | WCCGGW | 1 cut(s) | 1241 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 1220, 1368 |
| Bse118I | RCCGGY | 2 cut(s) | 34, 1241 |
| Bse1I | ACTGG | 2 cut(s) | 113, 1011 |
| Bse21I | CCTNAGG | 1 cut(s) | 1113 |
| Bse3DI | GCAATG | 1 cut(s) | 1278 |
| Bse8I | GATNNNNATC | 1 cut(s) | 923 |
| BseDI | CCNNGG | 2 cut(s) | 43, 436 |
| BseGI | GGATG | 2 cut(s) | 208, 807 |
| BseJI | GATNNNNATC | 1 cut(s) | 923 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 1220, 1368 |
| BseMI | GCAATG | 1 cut(s) | 1278 |
| BseMII | CTCAG | 3 cut(s) | 987, 1104, 1265 |
| BseNI | ACTGG | 2 cut(s) | 113, 1011 |
| BseSI | GKGCMC | 2 cut(s) | 981, 1249 |
| BseXI | GCAGC | 4 cut(s) | 413, 711, 880, 1369 |
| BsgI | GTGCAG | 1 cut(s) | 932 |
| BshFI | GGCC | 2 cut(s) | 358, 1110 |
| BshNI | GGYRCC | 1 cut(s) | 1065 |
| BshTI | ACCGGT | 1 cut(s) | 1241 |
| BsiHKAI | GWGCWC | 2 cut(s) | 466, 1249 |
| BsiHKCI | CYCGRG | 1 cut(s) | 43 |
| BsiSI | CCGG | 4 cut(s) | 35, 44, 675, 1242 |
| BslFI | GGGAC | 1 cut(s) | 245 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 1220, 1368 |
| BsmAI | GTCTC | 3 cut(s) | 482, 976, 1036 |
| BsmBI | CGTCTC | 1 cut(s) | 976 |
| BsmFI | GGGAC | 1 cut(s) | 245 |
| BsnI | GGCC | 2 cut(s) | 358, 1110 |
| BsoBI | CYCGRG | 1 cut(s) | 43 |
| Bsp1286I | GDGCHC | 4 cut(s) | 44, 466, 981, 1249 |
| Bsp1407I | TGTACA | 1 cut(s) | 816 |
| Bsp143I | GATC | 4 cut(s) | 423, 554, 655, 924 |
| BspACI | CCGC | 2 cut(s) | 864, 896 |
| BspANI | GGCC | 2 cut(s) | 358, 1110 |
| BspCNI | CTCAG | 3 cut(s) | 986, 1105, 1264 |
| BspLI | GGNNCC | 1 cut(s) | 1067 |
| BspPI | GGATC | 2 cut(s) | 418, 549 |
| BspQI | GCTCTTC | 1 cut(s) | 471 |
| BspT107I | GGYRCC | 1 cut(s) | 1065 |
| BsrDI | GCAATG | 1 cut(s) | 1278 |
| BsrFI | RCCGGY | 2 cut(s) | 34, 1241 |
| BsrGI | TGTACA | 1 cut(s) | 816 |
| BsrI | ACTGG | 2 cut(s) | 113, 1011 |
| BssAI | RCCGGY | 2 cut(s) | 34, 1241 |
| BssECI | CCNNGG | 2 cut(s) | 43, 436 |
| BssMI | GATC | 4 cut(s) | 423, 554, 655, 924 |
| BssT1I | CCWWGG | 1 cut(s) | 436 |
| Bst4CI | ACNGT | 1 cut(s) | 605 |
| Bst6I | CTCTTC | 5 cut(s) | 184, 218, 471, 507, 1060 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 89 |
| BstAUI | TGTACA | 1 cut(s) | 816 |
| BstC8I | GCNNGC | 3 cut(s) | 356, 1344, 1348 |
| BstDEI | CTNAG | 5 cut(s) | 579, 973, 1113, 1251, 1292 |
| BstF5I | GGATG | 2 cut(s) | 208, 807 |
| BstKTI | GATC | 4 cut(s) | 426, 557, 658, 927 |
| BstMAI | GTCTC | 3 cut(s) | 482, 976, 1036 |
| BstMBI | GATC | 4 cut(s) | 423, 554, 655, 924 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 89, 858, 1268, 1307 |
| BstNSI | RCATGY | 2 cut(s) | 260, 1350 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 703 |
| BstSCI | CCNGG | 2 cut(s) | 42, 43 |
| BstSLI | GKGCMC | 2 cut(s) | 981, 1249 |
| BstV1I | GCAGC | 4 cut(s) | 413, 711, 880, 1369 |
| BstV2I | GAAGAC | 2 cut(s) | 145, 252 |
| BstX2I | RGATCY | 1 cut(s) | 423 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 437 |
| BstYI | RGATCY | 1 cut(s) | 423 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1113 |
| BsuRI | GGCC | 2 cut(s) | 358, 1110 |
| BtgZI | GCGATG | 1 cut(s) | 456 |
| BtsCI | GGATG | 2 cut(s) | 208, 807 |
| BtsI | GCAGTG | 1 cut(s) | 224 |
| BtsIMutI | CAGTG | 6 cut(s) | 224, 915, 981, 1004, 1142, 1259 |
| Cac8I | GCNNGC | 3 cut(s) | 356, 1344, 1348 |
| Cfr10I | RCCGGY | 2 cut(s) | 34, 1241 |
| Cfr13I | GGNCC | 3 cut(s) | 672, 1109, 1181 |
| Cfr9I | CCCGGG | 1 cut(s) | 43 |
| Csp6I | GTAC | 3 cut(s) | 385, 817, 1066 |
| CspAI | ACCGGT | 1 cut(s) | 1241 |
| CviQI | GTAC | 3 cut(s) | 385, 817, 1066 |
| DdeI | CTNAG | 5 cut(s) | 579, 973, 1113, 1251, 1292 |
| DpnI | GATC | 4 cut(s) | 425, 556, 657, 926 |
| DpnII | GATC | 4 cut(s) | 423, 554, 655, 924 |
| Eam1104I | CTCTTC | 5 cut(s) | 184, 218, 471, 507, 1060 |
| EarI | CTCTTC | 5 cut(s) | 184, 218, 471, 507, 1060 |
| Eco130I | CCWWGG | 1 cut(s) | 436 |
| Eco24I | GRGCYC | 1 cut(s) | 44 |
| Eco47I | GGWCC | 2 cut(s) | 672, 1181 |
| Eco81I | CCTNAGG | 1 cut(s) | 1113 |
| Eco88I | CYCGRG | 1 cut(s) | 43 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1109 |
| EcoT14I | CCWWGG | 1 cut(s) | 436 |
| EcoT22I | ATGCAT | 1 cut(s) | 1352 |
| EcoT38I | GRGCYC | 1 cut(s) | 44 |
| ErhI | CCWWGG | 1 cut(s) | 436 |
| Esp3I | CGTCTC | 1 cut(s) | 976 |
| FaqI | GGGAC | 1 cut(s) | 245 |
| FauI | CCCGC | 1 cut(s) | 889 |
| FblI | GTMKAC | 3 cut(s) | 117, 713, 886 |
| Fnu4HI | GCNGC | 4 cut(s) | 402, 725, 894, 1383 |
| FokI | GGATG | 2 cut(s) | 215, 794 |
| FriOI | GRGCYC | 1 cut(s) | 44 |
| Fsp4HI | GCNGC | 4 cut(s) | 402, 725, 894, 1383 |
| FspBI | CTAG | 1 cut(s) | 1077 |
| GluI | GCNGC | 4 cut(s) | 402, 725, 894, 1383 |
| HaeIII | GGCC | 2 cut(s) | 358, 1110 |
| HapII | CCGG | 4 cut(s) | 35, 44, 675, 1242 |
| HincII | GTYRAC | 2 cut(s) | 118, 714 |
| HindII | GTYRAC | 2 cut(s) | 118, 714 |
| HindIII | AAGCTT | 1 cut(s) | 758 |
| HinfI | GANTC | 5 cut(s) | 101, 122, 290, 614, 1164 |
| HpaII | CCGG | 4 cut(s) | 35, 44, 675, 1242 |
| HphI | GGTGA | 3 cut(s) | 49, 1063, 1202 |
| Hpy166II | GTNNAC | 4 cut(s) | 118, 714, 887, 1247 |
| Hpy188I | TCNGA | 2 cut(s) | 337, 812 |
| Hpy188III | TCNNGA | 7 cut(s) | 160, 276, 421, 751, 789, 824, 1039 |
| Hpy8I | GTNNAC | 4 cut(s) | 118, 714, 887, 1247 |
| Hpy99I | CGWCG | 1 cut(s) | 122 |
| HpyAV | CCTTC | 5 cut(s) | 8, 188, 507, 528, 628 |
| HpyCH4III | ACNGT | 1 cut(s) | 605 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 89, 858, 1268, 1307 |
| HpyF3I | CTNAG | 5 cut(s) | 579, 973, 1113, 1251, 1292 |
| KpnI | GGTACC | 1 cut(s) | 1069 |
| Kzo9I | GATC | 4 cut(s) | 423, 554, 655, 924 |
| LguI | GCTCTTC | 1 cut(s) | 471 |
| LmnI | GCTCC | 3 cut(s) | 144, 624, 1307 |
| Lsp1109I | GCAGC | 4 cut(s) | 413, 711, 880, 1369 |
| LweI | GCATC | 3 cut(s) | 341, 816, 1348 |
| MaeI | CTAG | 1 cut(s) | 1077 |
| MaeIII | GTNAC | 5 cut(s) | 282, 766, 899, 994, 1237 |
| MalI | GATC | 4 cut(s) | 425, 556, 657, 926 |
| MboI | GATC | 4 cut(s) | 423, 554, 655, 924 |
| MflI | RGATCY | 1 cut(s) | 423 |
| MhlI | GDGCHC | 4 cut(s) | 44, 466, 981, 1249 |
| MluCI | AATT | 8 cut(s) | 53, 84, 738, 856, 954, 1100, 1224, 1391 |
| MmeI | TCCRAC | 1 cut(s) | 602 |
| MnlI | CCTC | 9 cut(s) | 11, 167, 219, 283, 727, 1079, 1100, 1108, 1260 |
| Mph1103I | ATGCAT | 1 cut(s) | 1352 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1054 |
| MseI | TTAA | 4 cut(s) | 144, 165, 741, 1218 |
| MslI | CAYNNNNRTG | 4 cut(s) | 255, 812, 1172, 1252 |
| MspA1I | CMGCKG | 1 cut(s) | 404 |
| MspI | CCGG | 4 cut(s) | 35, 44, 675, 1242 |
| MspR9I | CCNGG | 2 cut(s) | 44, 45 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 89, 858, 1268, 1307 |
| NciI | CCSGG | 2 cut(s) | 44, 45 |
| NdeII | GATC | 4 cut(s) | 423, 554, 655, 924 |
| NlaIV | GGNNCC | 1 cut(s) | 1067 |
| NmuCI | GTSAC | 3 cut(s) | 282, 766, 1237 |
| NsiI | ATGCAT | 1 cut(s) | 1352 |
| NspI | RCATGY | 2 cut(s) | 260, 1350 |
| OliI | CACNNNNGTG | 1 cut(s) | 1252 |
| PaeI | GCATGC | 1 cut(s) | 1350 |
| PciSI | GCTCTTC | 1 cut(s) | 471 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1054 |
| PfeI | GAWTC | 5 cut(s) | 101, 122, 290, 614, 1164 |
| PinAI | ACCGGT | 1 cut(s) | 1241 |
| PkrI | GCNGC | 4 cut(s) | 403, 726, 895, 1384 |
| PshAI | GACNNNNGTC | 1 cut(s) | 703 |
| PshBI | ATTAAT | 1 cut(s) | 165 |
| PspN4I | GGNNCC | 1 cut(s) | 1067 |
| PspPI | GGNCC | 3 cut(s) | 672, 1109, 1181 |
| PsuI | RGATCY | 1 cut(s) | 423 |
| PvuII | CAGCTG | 1 cut(s) | 404 |
| RsaI | GTAC | 3 cut(s) | 386, 818, 1067 |
| RsaNI | GTAC | 3 cut(s) | 385, 817, 1066 |
| RseI | CAYNNNNRTG | 4 cut(s) | 255, 812, 1172, 1252 |
| SalI | GTCGAC | 2 cut(s) | 116, 712 |
| SapI | GCTCTTC | 1 cut(s) | 471 |
| SaqAI | TTAA | 4 cut(s) | 144, 165, 741, 1218 |
| SatI | GCNGC | 4 cut(s) | 402, 725, 894, 1383 |
| Sau3AI | GATC | 4 cut(s) | 423, 554, 655, 924 |
| Sau96I | GGNCC | 3 cut(s) | 672, 1109, 1181 |
| ScrFI | CCNGG | 2 cut(s) | 44, 45 |
| SduI | GDGCHC | 4 cut(s) | 44, 466, 981, 1249 |
| SfaNI | GCATC | 3 cut(s) | 341, 816, 1348 |
| SinI | GGWCC | 2 cut(s) | 672, 1181 |
| SmaI | CCCGGG | 1 cut(s) | 45 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 255, 812, 1172, 1252 |
| SmlI | CTYRAG | 1 cut(s) | 1153 |
| SmoI | CTYRAG | 1 cut(s) | 1153 |
| SphI | GCATGC | 1 cut(s) | 1350 |
| Sse9I | AATT | 8 cut(s) | 53, 84, 738, 856, 954, 1100, 1224, 1391 |
| SsiI | CCGC | 2 cut(s) | 864, 896 |
| SspI | AATATT | 1 cut(s) | 417 |
| SspMI | CTAG | 1 cut(s) | 1077 |
| StyD4I | CCNGG | 2 cut(s) | 42, 43 |
| StyI | CCWWGG | 1 cut(s) | 436 |
| TaaI | ACNGT | 1 cut(s) | 605 |
| TaqI | TCGA | 6 cut(s) | 117, 553, 658, 697, 713, 927 |
| TasI | AATT | 8 cut(s) | 53, 84, 738, 856, 954, 1100, 1224, 1391 |
| TatI | WGTACW | 1 cut(s) | 816 |
| TfiI | GAWTC | 5 cut(s) | 101, 122, 290, 614, 1164 |
| Tru1I | TTAA | 4 cut(s) | 144, 165, 741, 1218 |
| Tru9I | TTAA | 4 cut(s) | 144, 165, 741, 1218 |
| TscAI | CASTG | 6 cut(s) | 224, 915, 981, 1011, 1149, 1259 |
| TseFI | GTSAC | 3 cut(s) | 282, 766, 1237 |
| TseI | GCWGC | 4 cut(s) | 401, 724, 893, 1382 |
| Tsp45I | GTSAC | 3 cut(s) | 282, 766, 1237 |
| TspDTI | ATGAA | 8 cut(s) | 219, 362, 555, 606, 852, 1047, 1312, 1404 |
| TspGWI | ACGGA | 1 cut(s) | 697 |
| TspMI | CCCGGG | 1 cut(s) | 43 |
| TspRI | CASTG | 6 cut(s) | 224, 915, 981, 1011, 1149, 1259 |
| VneI | GTGCAC | 1 cut(s) | 1245 |
| VpaK11BI | GGWCC | 2 cut(s) | 672, 1181 |
| VspI | ATTAAT | 1 cut(s) | 165 |
| XapI | RAATTY | 3 cut(s) | 1100, 1224, 1391 |
| XceI | RCATGY | 2 cut(s) | 260, 1350 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 434, 1081 |
| XmaI | CCCGGG | 1 cut(s) | 43 |
| XmiI | GTMKAC | 3 cut(s) | 117, 713, 886 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1054 |
| XspI | CTAG | 1 cut(s) | 1077 |
| Zsp2I | ATGCAT | 1 cut(s) | 1352 |