O-acyltransferase (WSD1-like)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 122 |
| AccII | CGCG | 1 cut(s) | 761 |
| AciI | CCGC | 2 cut(s) | 344, 759 |
| AclWI | GGATC | 1 cut(s) | 1310 |
| AcsI | RAATTY | 5 cut(s) | 366, 550, 564, 1020, 1088 |
| AcuI | CTGAAG | 1 cut(s) | 858 |
| AfaI | GTAC | 3 cut(s) | 59, 383, 942 |
| AfeI | AGCGCT | 1 cut(s) | 432 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 122, 1208 |
| AgeI | ACCGGT | 1 cut(s) | 1171 |
| AgsI | TTSAA | 9 cut(s) | 149, 230, 242, 418, 583, 667, 1135, 1294, 1330 |
| AluBI | AGCT | 5 cut(s) | 69, 134, 238, 421, 485 |
| AluI | AGCT | 5 cut(s) | 69, 134, 238, 421, 485 |
| Alw21I | GWGCWC | 1 cut(s) | 463 |
| Alw26I | GTCTC | 1 cut(s) | 791 |
| AlwI | GGATC | 1 cut(s) | 1310 |
| Aor51HI | AGCGCT | 1 cut(s) | 432 |
| AoxI | GGCC | 3 cut(s) | 274, 1096, 1243 |
| ApeKI | GCWGC | 5 cut(s) | 34, 398, 532, 1106, 1109 |
| ApoI | RAATTY | 5 cut(s) | 366, 550, 564, 1020, 1088 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1316, 1348 |
| AsiGI | ACCGGT | 1 cut(s) | 1171 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 339 |
| AspLEI | GCGC | 1 cut(s) | 433 |
| AspS9I | GGNCC | 2 cut(s) | 1169, 1243 |
| AsuHPI | GGTGA | 5 cut(s) | 52, 416, 449, 1227, 1291 |
| AvaII | GGWCC | 1 cut(s) | 1169 |
| AxyI | CCTNAGG | 1 cut(s) | 1239 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1108, 1141 |
| BanII | GRGCYC | 1 cut(s) | 47 |
| BbsI | GAAGAC | 2 cut(s) | 249, 1016 |
| Bbv12I | GWGCWC | 1 cut(s) | 463 |
| BbvI | GCAGC | 5 cut(s) | 46, 410, 544, 1118, 1121 |
| BccI | CCATC | 6 cut(s) | 116, 173, 434, 647, 853, 1291 |
| BclI | TGATCA | 2 cut(s) | 625, 888 |
| BcoDI | GTCTC | 1 cut(s) | 791 |
| BfaI | CTAG | 1 cut(s) | 66 |
| BfmI | CTRYAG | 1 cut(s) | 32 |
| BfoI | RGCGCY | 1 cut(s) | 434 |
| BisI | GCNGC | 5 cut(s) | 35, 399, 533, 1107, 1110 |
| BlsI | GCNGC | 5 cut(s) | 36, 400, 534, 1108, 1111 |
| BmcAI | AGTACT | 1 cut(s) | 59 |
| Bme18I | GGWCC | 1 cut(s) | 1169 |
| BmgT120I | GGNCC | 2 cut(s) | 1169, 1243 |
| BmiI | GGNNCC | 2 cut(s) | 114, 920 |
| BmsI | GCATC | 1 cut(s) | 296 |
| BpiI | GAAGAC | 2 cut(s) | 249, 1016 |
| Bsa29I | ATCGAT | 1 cut(s) | 120 |
| BsaAI | YACGTR | 1 cut(s) | 1124 |
| BsaBI | GATNNNNATC | 1 cut(s) | 300 |
| BsaJI | CCNNGG | 5 cut(s) | 173, 536, 772, 911, 1371 |
| BsaWI | WCCGGW | 1 cut(s) | 1171 |
| BsaXI | ACNNNNNCTCC | 1 cut(s) | 26 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 122, 1208 |
| Bse118I | RCCGGY | 3 cut(s) | 37, 401, 1171 |
| Bse1I | ACTGG | 1 cut(s) | 1202 |
| Bse21I | CCTNAGG | 1 cut(s) | 1239 |
| Bse3DI | GCAATG | 1 cut(s) | 336 |
| Bse8I | GATNNNNATC | 1 cut(s) | 300 |
| BseCI | ATCGAT | 1 cut(s) | 120 |
| BseDI | CCNNGG | 5 cut(s) | 173, 536, 772, 911, 1371 |
| BseGI | GGATG | 4 cut(s) | 255, 789, 861, 1198 |
| BseJI | GATNNNNATC | 1 cut(s) | 300 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 122, 1208 |
| BseMI | GCAATG | 1 cut(s) | 336 |
| BseMII | CTCAG | 2 cut(s) | 99, 1230 |
| BseNI | ACTGG | 1 cut(s) | 1202 |
| BseRI | GAGGAG | 1 cut(s) | 172 |
| BseXI | GCAGC | 5 cut(s) | 46, 410, 544, 1118, 1121 |
| BseYI | CCCAGC | 1 cut(s) | 1064 |
| Bsh1236I | CGCG | 1 cut(s) | 761 |
| BshFI | GGCC | 3 cut(s) | 276, 1098, 1245 |
| BshTI | ACCGGT | 1 cut(s) | 1171 |
| BshVI | ATCGAT | 1 cut(s) | 120 |
| BsiHKAI | GWGCWC | 1 cut(s) | 463 |
| BsiSI | CCGG | 3 cut(s) | 38, 402, 1172 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 122, 1208 |
| BsmAI | GTCTC | 1 cut(s) | 791 |
| BsnI | GGCC | 3 cut(s) | 276, 1098, 1245 |
| Bsp1286I | GDGCHC | 2 cut(s) | 47, 463 |
| Bsp143I | GATC | 6 cut(s) | 186, 625, 641, 765, 888, 1315 |
| Bsp19I | CCATGG | 2 cut(s) | 536, 911 |
| BspACI | CCGC | 2 cut(s) | 344, 759 |
| BspANI | GGCC | 3 cut(s) | 276, 1098, 1245 |
| BspCNI | CTCAG | 2 cut(s) | 100, 1231 |
| BspDI | ATCGAT | 1 cut(s) | 120 |
| BspFNI | CGCG | 1 cut(s) | 761 |
| BspLI | GGNNCC | 2 cut(s) | 114, 920 |
| BspMAI | CTGCAG | 1 cut(s) | 36 |
| BspPI | GGATC | 1 cut(s) | 1310 |
| BspQI | GCTCTTC | 1 cut(s) | 468 |
| BsrDI | GCAATG | 1 cut(s) | 336 |
| BsrFI | RCCGGY | 3 cut(s) | 37, 401, 1171 |
| BsrI | ACTGG | 1 cut(s) | 1202 |
| BssAI | RCCGGY | 3 cut(s) | 37, 401, 1171 |
| BssECI | CCNNGG | 5 cut(s) | 173, 536, 772, 911, 1371 |
| BssMI | GATC | 6 cut(s) | 186, 625, 641, 765, 888, 1315 |
| BssT1I | CCWWGG | 3 cut(s) | 536, 772, 911 |
| Bst4CI | ACNGT | 3 cut(s) | 177, 409, 933 |
| Bst6I | CTCTTC | 3 cut(s) | 468, 504, 536 |
| BstBAI | YACGTR | 1 cut(s) | 1124 |
| BstC8I | GCNNGC | 3 cut(s) | 285, 403, 728 |
| BstDEI | CTNAG | 2 cut(s) | 108, 1239 |
| BstDSI | CCRYGG | 3 cut(s) | 173, 536, 911 |
| BstF5I | GGATG | 4 cut(s) | 255, 789, 861, 1198 |
| BstFNI | CGCG | 1 cut(s) | 761 |
| BstH2I | RGCGCY | 1 cut(s) | 434 |
| BstHHI | GCGC | 1 cut(s) | 433 |
| BstKTI | GATC | 6 cut(s) | 189, 628, 644, 768, 891, 1318 |
| BstMAI | GTCTC | 1 cut(s) | 791 |
| BstMBI | GATC | 6 cut(s) | 186, 625, 641, 765, 888, 1315 |
| BstNSI | RCATGY | 1 cut(s) | 941 |
| BstSFI | CTRYAG | 1 cut(s) | 32 |
| BstSNI | TACGTA | 1 cut(s) | 1124 |
| BstUI | CGCG | 1 cut(s) | 761 |
| BstV1I | GCAGC | 5 cut(s) | 46, 410, 544, 1118, 1121 |
| BstV2I | GAAGAC | 2 cut(s) | 249, 1016 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 434 |
| Bsu15I | ATCGAT | 1 cut(s) | 120 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1239 |
| BsuRI | GGCC | 3 cut(s) | 276, 1098, 1245 |
| BsuTUI | ATCGAT | 1 cut(s) | 120 |
| BtgI | CCRYGG | 3 cut(s) | 173, 536, 911 |
| BtgZI | GCGATG | 1 cut(s) | 776 |
| BtsCI | GGATG | 4 cut(s) | 255, 789, 861, 1198 |
| BtsI | GCAGTG | 2 cut(s) | 221, 1059 |
| BtsIMutI | CAGTG | 4 cut(s) | 221, 405, 929, 1059 |
| Cac8I | GCNNGC | 3 cut(s) | 285, 403, 728 |
| CfoI | GCGC | 1 cut(s) | 433 |
| Cfr10I | RCCGGY | 3 cut(s) | 37, 401, 1171 |
| Cfr13I | GGNCC | 2 cut(s) | 1169, 1243 |
| ClaI | ATCGAT | 1 cut(s) | 120 |
| Csp6I | GTAC | 3 cut(s) | 58, 382, 941 |
| CspAI | ACCGGT | 1 cut(s) | 1171 |
| CviQI | GTAC | 3 cut(s) | 58, 382, 941 |
| DdeI | CTNAG | 2 cut(s) | 108, 1239 |
| DpnI | GATC | 6 cut(s) | 188, 627, 643, 767, 890, 1317 |
| DpnII | GATC | 6 cut(s) | 186, 625, 641, 765, 888, 1315 |
| DraI | TTTAAA | 1 cut(s) | 603 |
| Eam1104I | CTCTTC | 3 cut(s) | 468, 504, 536 |
| EarI | CTCTTC | 3 cut(s) | 468, 504, 536 |
| Eco105I | TACGTA | 1 cut(s) | 1124 |
| Eco130I | CCWWGG | 3 cut(s) | 536, 772, 911 |
| Eco24I | GRGCYC | 1 cut(s) | 47 |
| Eco47I | GGWCC | 1 cut(s) | 1169 |
| Eco47III | AGCGCT | 1 cut(s) | 432 |
| Eco57I | CTGAAG | 1 cut(s) | 858 |
| Eco81I | CCTNAGG | 1 cut(s) | 1239 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1243 |
| EcoT14I | CCWWGG | 3 cut(s) | 536, 772, 911 |
| EcoT22I | ATGCAT | 1 cut(s) | 1340 |
| EcoT38I | GRGCYC | 1 cut(s) | 47 |
| ErhI | CCWWGG | 3 cut(s) | 536, 772, 911 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 222, 254, 1027, 1059 |
| FbaI | TGATCA | 2 cut(s) | 625, 888 |
| Fnu4HI | GCNGC | 5 cut(s) | 35, 399, 533, 1107, 1110 |
| FokI | GGATG | 4 cut(s) | 242, 776, 868, 1185 |
| FriOI | GRGCYC | 1 cut(s) | 47 |
| Fsp4HI | GCNGC | 5 cut(s) | 35, 399, 533, 1107, 1110 |
| FspBI | CTAG | 1 cut(s) | 66 |
| GlaI | GCGC | 1 cut(s) | 432 |
| GluI | GCNGC | 5 cut(s) | 35, 399, 533, 1107, 1110 |
| GsaI | CCCAGC | 1 cut(s) | 1068 |
| HaeII | RGCGCY | 1 cut(s) | 434 |
| HaeIII | GGCC | 3 cut(s) | 276, 1098, 1245 |
| HapII | CCGG | 3 cut(s) | 38, 402, 1172 |
| HhaI | GCGC | 1 cut(s) | 433 |
| Hin6I | GCGC | 1 cut(s) | 431 |
| HinP1I | GCGC | 1 cut(s) | 431 |
| HincII | GTYRAC | 2 cut(s) | 253, 960 |
| HindII | GTYRAC | 2 cut(s) | 253, 960 |
| HindIII | AAGCTT | 2 cut(s) | 236, 419 |
| HinfI | GANTC | 3 cut(s) | 88, 708, 1078 |
| HpaI | GTTAAC | 2 cut(s) | 253, 960 |
| HpaII | CCGG | 3 cut(s) | 38, 402, 1172 |
| HphI | GGTGA | 5 cut(s) | 52, 416, 449, 1227, 1291 |
| Hpy166II | GTNNAC | 4 cut(s) | 253, 476, 960, 1235 |
| Hpy188I | TCNGA | 5 cut(s) | 595, 641, 734, 985, 1375 |
| Hpy188III | TCNNGA | 6 cut(s) | 305, 519, 721, 881, 1006, 1294 |
| Hpy8I | GTNNAC | 4 cut(s) | 253, 476, 960, 1235 |
| HpyAV | CCTTC | 7 cut(s) | 20, 195, 279, 504, 516, 520, 1300 |
| HpyCH4III | ACNGT | 3 cut(s) | 177, 409, 933 |
| HpyCH4IV | ACGT | 2 cut(s) | 750, 1123 |
| HpyCH4V | TGCA | 9 cut(s) | 10, 34, 287, 398, 677, 937, 992, 1338, 1390 |
| HpyF3I | CTNAG | 2 cut(s) | 108, 1239 |
| HpySE526I | ACGT | 2 cut(s) | 750, 1123 |
| HspAI | GCGC | 1 cut(s) | 431 |
| KroI | GCCGGC | 1 cut(s) | 401 |
| KroNI | GCCGGC | 1 cut(s) | 403 |
| Ksp22I | TGATCA | 2 cut(s) | 625, 888 |
| KspAI | GTTAAC | 2 cut(s) | 253, 960 |
| Kzo9I | GATC | 6 cut(s) | 186, 625, 641, 765, 888, 1315 |
| LguI | GCTCTTC | 1 cut(s) | 468 |
| LmnI | GCTCC | 1 cut(s) | 1029 |
| Lsp1109I | GCAGC | 5 cut(s) | 46, 410, 544, 1118, 1121 |
| LweI | GCATC | 1 cut(s) | 296 |
| MaeI | CTAG | 1 cut(s) | 66 |
| MaeII | ACGT | 2 cut(s) | 750, 1123 |
| MaeIII | GTNAC | 5 cut(s) | 556, 736, 927, 1124, 1252 |
| MalI | GATC | 6 cut(s) | 188, 627, 643, 767, 890, 1317 |
| MboI | GATC | 6 cut(s) | 186, 625, 641, 765, 888, 1315 |
| MfeI | CAATTG | 2 cut(s) | 672, 834 |
| MhlI | GDGCHC | 2 cut(s) | 47, 463 |
| Mph1103I | ATGCAT | 1 cut(s) | 1340 |
| MroNI | GCCGGC | 1 cut(s) | 401 |
| MroXI | GAANNNNTTC | 1 cut(s) | 339 |
| MseI | TTAA | 7 cut(s) | 252, 602, 812, 959, 1025, 1206, 1399 |
| MslI | CAYNNNNRTG | 1 cut(s) | 854 |
| MspI | CCGG | 3 cut(s) | 38, 402, 1172 |
| MunI | CAATTG | 2 cut(s) | 672, 834 |
| MvnI | CGCG | 1 cut(s) | 761 |
| NaeI | GCCGGC | 1 cut(s) | 403 |
| NcoI | CCATGG | 2 cut(s) | 536, 911 |
| NdeII | GATC | 6 cut(s) | 186, 625, 641, 765, 888, 1315 |
| NgoMIV | GCCGGC | 1 cut(s) | 401 |
| NlaIV | GGNNCC | 2 cut(s) | 114, 920 |
| NmuCI | GTSAC | 3 cut(s) | 556, 736, 927 |
| NsiI | ATGCAT | 1 cut(s) | 1340 |
| NspI | RCATGY | 1 cut(s) | 941 |
| PciSI | GCTCTTC | 1 cut(s) | 468 |
| PdiI | GCCGGC | 1 cut(s) | 403 |
| PdmI | GAANNNNTTC | 1 cut(s) | 339 |
| PfeI | GAWTC | 3 cut(s) | 88, 708, 1078 |
| PflFI | GACNNNGTC | 1 cut(s) | 1001 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 122 |
| PinAI | ACCGGT | 1 cut(s) | 1171 |
| PkrI | GCNGC | 5 cut(s) | 36, 400, 534, 1108, 1111 |
| Ppu21I | YACGTR | 1 cut(s) | 1124 |
| PspFI | CCCAGC | 1 cut(s) | 1064 |
| PspN4I | GGNNCC | 2 cut(s) | 114, 920 |
| PspPI | GGNCC | 2 cut(s) | 1169, 1243 |
| PstI | CTGCAG | 1 cut(s) | 36 |
| PsyI | GACNNNGTC | 1 cut(s) | 1001 |
| RsaI | GTAC | 3 cut(s) | 59, 383, 942 |
| RsaNI | GTAC | 3 cut(s) | 58, 382, 941 |
| RseI | CAYNNNNRTG | 1 cut(s) | 854 |
| SapI | GCTCTTC | 1 cut(s) | 468 |
| SaqAI | TTAA | 7 cut(s) | 252, 602, 812, 959, 1025, 1206, 1399 |
| SatI | GCNGC | 5 cut(s) | 35, 399, 533, 1107, 1110 |
| Sau3AI | GATC | 6 cut(s) | 186, 625, 641, 765, 888, 1315 |
| Sau96I | GGNCC | 2 cut(s) | 1169, 1243 |
| ScaI | AGTACT | 1 cut(s) | 59 |
| SduI | GDGCHC | 2 cut(s) | 47, 463 |
| SfaNI | GCATC | 1 cut(s) | 296 |
| SfcI | CTRYAG | 1 cut(s) | 32 |
| SinI | GGWCC | 1 cut(s) | 1169 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 854 |
| SnaBI | TACGTA | 1 cut(s) | 1124 |
| SsiI | CCGC | 2 cut(s) | 344, 759 |
| SspI | AATATT | 1 cut(s) | 414 |
| SspMI | CTAG | 1 cut(s) | 66 |
| StyI | CCWWGG | 3 cut(s) | 536, 772, 911 |
| TaaI | ACNGT | 3 cut(s) | 177, 409, 933 |
| TaiI | ACGT | 2 cut(s) | 753, 1126 |
| TaqI | TCGA | 6 cut(s) | 86, 120, 185, 781, 1142, 1365 |
| TaqII | GACCGA | 1 cut(s) | 41 |
| TatI | WGTACW | 1 cut(s) | 57 |
| TfiI | GAWTC | 3 cut(s) | 88, 708, 1078 |
| Tru1I | TTAA | 7 cut(s) | 252, 602, 812, 959, 1025, 1206, 1399 |
| Tru9I | TTAA | 7 cut(s) | 252, 602, 812, 959, 1025, 1206, 1399 |
| TscAI | CASTG | 4 cut(s) | 221, 412, 936, 1066 |
| TseFI | GTSAC | 3 cut(s) | 556, 736, 927 |
| TseI | GCWGC | 5 cut(s) | 34, 398, 532, 1106, 1109 |
| Tsp45I | GTSAC | 3 cut(s) | 556, 736, 927 |
| TspDTI | ATGAA | 1 cut(s) | 1043 |
| TspRI | CASTG | 4 cut(s) | 221, 412, 936, 1066 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1001 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 122 |
| VpaK11BI | GGWCC | 1 cut(s) | 1169 |
| XapI | RAATTY | 5 cut(s) | 366, 550, 564, 1020, 1088 |
| XceI | RCATGY | 1 cut(s) | 941 |
| XmnI | GAANNNNTTC | 1 cut(s) | 339 |
| XspI | CTAG | 1 cut(s) | 66 |
| ZrmI | AGTACT | 1 cut(s) | 59 |
| Zsp2I | ATGCAT | 1 cut(s) | 1340 |