Rroxscaffold_1G00029740

Wax ester synthase-like Acyl-CoA acyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000634
Physical Location & Seq
Forward (+)
38586051 .. 38595365
9315 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00029740.1

Sequence Viewer

Length: 759 bp
ATGGGCAATCTCGACATATATGCAGAGTTGTCTCCACCGCATTTGTACAAAGTACACACTCTTGTGTTTCCAACTACACTCTTCGAGAAAATAACCTCTCTCTCGGAAGCAATCACAAATATGATTGGGCCATTGGAACGAATGAGTCTCGCCAATCACCCCATTGAAGGAATTTACTTCATAGTGACGGGTTCACCTCAGGTTGAACGAGATGTATGTGTGCATCCTAATCCATCTCAAGCTAAACCCAGCCGAAGAGTAAGAATATTAGCTAGAGCAACCCATGTCCAGTCGTCGACCTCCAAGCCATCAACCAAGACAATCCCGCCATATATTCATGCAATGAGTTATATGGGAAAGCTAAGGATTGCTTTGGCAGCTGAAAAAGACTTCATAGATACCAACGTATTGCAAGCATGCATGAAAGATGCATTTCGGGTGATATGTGAAGCTGTTCGTACAGTACTCCCTGCTGCACCGGATTACTATCTCAAAATTGAAAACCCTAGCAAATCGCAAACTCCCATCGCCCTCTCATTTTCGCTTTTCACTCTTGCAGTCTTTGGGCTCTTTGCTAGCCTCCTCGGTCCCTCCCGAGTCACGACGAAGACGACGAAGACGAAGACGACGCCGACGACGAAGACGCCGACGACCGACGACGCTCATCCTCGGTCCTCCCGGGAGCTCATCTCTCGAATCACGACGCCGACGACGCCGACTACGCCGACGACGTCGACGACCGACGACGTCGAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1298 WES_acyltransf

Protein Analysis

252

Amino Acids

27.72

Weight (kDa)

9.1

Isoelectric Point (pI)

43.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WS_DGAT_C PF06974 31 - 67 6.6e-07 WS/DGAT C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000634)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10290 FvH4_3g28230 FvH4_3g28240
malus_domestica MD05G1036400.v1.1 MD10G1159400.v1.1 MD17G1237100.v1.1 MD17G1237200.v1.1 MD17G1237300.v1.1 MD17G1237600.v1.1 MD17G1238200.v1.1
prunus_persica Prupe.3G140400_v2.0.a1 Prupe.3G140700_v2.0.a1 Prupe.3G141100_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G258100_v2.0.a1 Prupe.7G117300_v2.0.a1 Prupe.7G117400_v2.0.a1 Prupe.7G117600_v2.0.a1 Prupe.8G044700_v2.0.a1
pyrus_communis pycom05g02680 pycom17g24220 pycom17g24240
rosa_chinensis RchiOBHm_Chr1g0350141 RchiOBHm_Chr5g0050931 RchiOBHm_Chr5g0051001 RchiOBHm_Chr5g0051021 RchiOBHm_Chr6g0267491
rosa_laevigata RLG00000013999 RLG00000028502 RLG00000034744 RLG00000034748 RLG00000034753
rosa_multiflora Rmu_sc0000446.1_g000056 Rmu_sc0000782.1_g000006 Rmu_sc0002951.1_g000001 Rmu_sc0003674.1_g000026 Rmu_sc0003756.1_g000004 Rmu_sc0011917.1_g000004 Rmu_sc0012005.1_g000019 Rmu_sc0040739.1_g000001
rosa_roxburghii Rroxscaffold_1G00029730 Rroxscaffold_1G00029740 Rroxscaffold_4G00305060 Rroxscaffold_7G00200710
rosa_rugosa Rorug01G0208400 Rorug05G0265200.1 Rorug05G0265300.1 Rorug05G0265400.1 Rorug05G0265500.1 Rorug05G0294000 Rorug06G0033100 Rorug06G0243300
rosa_samantha Rh1BG192100 Rh1BG192200 Rh1DG220600 Rh5AG270300 Rh5AG337200 Rh5AG337700 Rh5BG346700 Rh5BG347400 Rh5CG374300 Rh5DG359700 Rh5DG360500 Rh6AG154500 Rh6BG155100 Rh6BG362300 Rh6CG150300 Rh6DG140300 Rh6DG356300
rosa_wichuraiana Rw0G018820 Rw1G019350 Rw5G025300 Rw5G031770 Rw5G031820 Rw5G031870 Rw5G031910 Rw6G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 734, 750
AccI GTMKAC 2 cut(s) 296, 734
AciI CCGC 2 cut(s) 38, 326
AcsI RAATTY 1 cut(s) 171
AcyI GRCGYC 6 cut(s) 629, 644, 704, 713, 731, 747
AfaI GTAC 4 cut(s) 47, 54, 460, 465
AfiI CCNNNNNNNGG 1 cut(s) 167
AgsI TTSAA 3 cut(s) 167, 206, 500
AleI CACNNNNGTG 1 cut(s) 62
AluBI AGCT 6 cut(s) 242, 272, 361, 380, 452, 685
AluI AGCT 6 cut(s) 242, 272, 361, 380, 452, 685
Alw21I GWGCWC 1 cut(s) 687
Alw26I GTCTC 2 cut(s) 36, 152
Ama87I CYCGRG 2 cut(s) 594, 678
AoxI GGCC 1 cut(s) 128
ApeKI GCWGC 2 cut(s) 377, 473
ApoI RAATTY 1 cut(s) 171
Asp700I GAANNNNTTC 1 cut(s) 453
AspS9I GGNCC 3 cut(s) 128, 587, 672
AsuC2I CCSGG 2 cut(s) 679, 680
AsuHPI GGTGA 3 cut(s) 149, 186, 451
AsuNHI GCTAGC 1 cut(s) 575
AvaI CYCGRG 2 cut(s) 594, 678
AvaII GGWCC 2 cut(s) 587, 672
AxyI CCTNAGG 1 cut(s) 198
BaeI ACNNNNGTAYC 2 cut(s) 390, 423
BanII GRGCYC 2 cut(s) 570, 687
BbsI GAAGAC 4 cut(s) 614, 623, 629, 647
Bbv12I GWGCWC 1 cut(s) 687
BbvI GCAGC 2 cut(s) 389, 460
BccI CCATC 3 cut(s) 241, 316, 533
BcgI CGANNNNNNTGC 1 cut(s) 36
BcnI CCSGG 2 cut(s) 679, 680
BcoDI GTCTC 2 cut(s) 36, 152
BfaI CTAG 3 cut(s) 273, 507, 576
BisI GCNGC 2 cut(s) 378, 474
BlsI GCNGC 2 cut(s) 379, 475
BmcAI AGTACT 1 cut(s) 465
Bme1390I CCNGG 2 cut(s) 679, 680
Bme18I GGWCC 2 cut(s) 587, 672
BmeT110I CYCGRG 2 cut(s) 594, 678
BmgT120I GGNCC 3 cut(s) 128, 587, 672
BmiI GGNNCC 1 cut(s) 589
BmrFI CCNGG 2 cut(s) 679, 680
BmsI GCATC 2 cut(s) 232, 418
BmtI GCTAGC 1 cut(s) 579
BpiI GAAGAC 4 cut(s) 614, 623, 629, 647
BplI GAGNNNNNCTC 2 cut(s) 674, 706
Bpu10I CCTNAGC 1 cut(s) 362
BpuEI CTTGAG 1 cut(s) 222
BpuMI CCSGG 2 cut(s) 679, 680
BsaBI GATNNNNATC 1 cut(s) 486
BsaHI GRCGYC 6 cut(s) 629, 644, 704, 713, 731, 747
BsaJI CCNNGG 3 cut(s) 583, 668, 678
BsaWI WCCGGW 1 cut(s) 478
Bsc4I CCNNNNNNNGG 1 cut(s) 167
Bse1I ACTGG 1 cut(s) 289
Bse21I CCTNAGG 1 cut(s) 198
Bse3DI GCAATG 1 cut(s) 348
Bse8I GATNNNNATC 1 cut(s) 486
BseDI CCNNGG 3 cut(s) 583, 668, 678
BseGI GGATG 2 cut(s) 223, 664
BseJI GATNNNNATC 1 cut(s) 486
BseLI CCNNNNNNNGG 1 cut(s) 167
BseMI GCAATG 1 cut(s) 348
BseMII CTCAG 1 cut(s) 212
BseNI ACTGG 1 cut(s) 289
BseRI GAGGAG 1 cut(s) 572
BseXI GCAGC 2 cut(s) 389, 460
BseYI CCCAGC 1 cut(s) 248
BsgI GTGCAG 1 cut(s) 459
Bsh1285I CGRYCG 2 cut(s) 654, 741
BshFI GGCC 1 cut(s) 130
BsiEI CGRYCG 2 cut(s) 654, 741
BsiHKAI GWGCWC 1 cut(s) 687
BsiHKCI CYCGRG 2 cut(s) 594, 678
BsiSI CCGG 2 cut(s) 479, 679
BslFI GGGAC 1 cut(s) 573
BslI CCNNNNNNNGG 1 cut(s) 167
BsmAI GTCTC 2 cut(s) 36, 152
BsmFI GGGAC 1 cut(s) 573
BsnI GGCC 1 cut(s) 130
BsoBI CYCGRG 2 cut(s) 594, 678
Bsp1286I GDGCHC 2 cut(s) 570, 687
Bsp1407I TGTACA 1 cut(s) 45
BspACI CCGC 2 cut(s) 38, 326
BspANI GGCC 1 cut(s) 130
BspCNI CTCAG 1 cut(s) 211
BspLI GGNNCC 1 cut(s) 589
BspOI GCTAGC 1 cut(s) 579
BsrDI GCAATG 1 cut(s) 348
BsrGI TGTACA 1 cut(s) 45
BsrI ACTGG 1 cut(s) 289
BssECI CCNNGG 3 cut(s) 583, 668, 678
BssNI GRCGYC 6 cut(s) 629, 644, 704, 713, 731, 747
Bst4CI ACNGT 1 cut(s) 463
Bst6I CTCTTC 3 cut(s) 86, 250, 747
BstACI GRCGYC 6 cut(s) 629, 644, 704, 713, 731, 747
BstAUI TGTACA 1 cut(s) 45
BstC8I GCNNGC 3 cut(s) 414, 418, 577
BstDEI CTNAG 2 cut(s) 198, 362
BstF5I GGATG 2 cut(s) 223, 664
BstMAI GTCTC 2 cut(s) 36, 152
BstMCI CGRYCG 2 cut(s) 654, 741
BstMWI GCNNNNNNNGC 3 cut(s) 377, 712, 721
BstNSI RCATGY 1 cut(s) 420
BstSCI CCNGG 2 cut(s) 677, 678
BstV1I GCAGC 2 cut(s) 389, 460
BstV2I GAAGAC 4 cut(s) 614, 623, 629, 647
Bsu36I CCTNAGG 1 cut(s) 198
BsuRI GGCC 1 cut(s) 130
BtgZI GCGATG 1 cut(s) 511
BtsCI GGATG 2 cut(s) 223, 664
Cac8I GCNNGC 3 cut(s) 414, 418, 577
Cfr13I GGNCC 3 cut(s) 128, 587, 672
Cfr9I CCCGGG 1 cut(s) 678
CseI GACGC 5 cut(s) 637, 652, 668, 712, 721
Csp6I GTAC 4 cut(s) 46, 53, 459, 464
CspCI CAANNNNNGTGG 2 cut(s) 24, 59
CviAII CATG 4 cut(s) 284, 338, 417, 421
CviQI GTAC 4 cut(s) 46, 53, 459, 464
DdeI CTNAG 2 cut(s) 198, 362
Eam1104I CTCTTC 3 cut(s) 86, 250, 747
EarI CTCTTC 3 cut(s) 86, 250, 747
Ecl136II GAGCTC 1 cut(s) 685
Eco24I GRGCYC 2 cut(s) 570, 687
Eco47I GGWCC 2 cut(s) 587, 672
Eco53kI GAGCTC 1 cut(s) 685
Eco81I CCTNAGG 1 cut(s) 198
Eco88I CYCGRG 2 cut(s) 594, 678
EcoICRI GAGCTC 1 cut(s) 685
EcoT22I ATGCAT 2 cut(s) 422, 433
EcoT38I GRGCYC 2 cut(s) 570, 687
FaeI CATG 4 cut(s) 287, 341, 420, 424
FalI AAGNNNNNCTT 2 cut(s) 355, 387
FaqI GGGAC 1 cut(s) 573
FatI CATG 4 cut(s) 283, 337, 416, 420
FauI CCCGC 1 cut(s) 333
FblI GTMKAC 2 cut(s) 296, 734
Fnu4HI GCNGC 2 cut(s) 378, 474
FokI GGATG 2 cut(s) 210, 651
FriOI GRGCYC 2 cut(s) 570, 687
Fsp4HI GCNGC 2 cut(s) 378, 474
FspBI CTAG 3 cut(s) 273, 507, 576
GluI GCNGC 2 cut(s) 378, 474
GsaI CCCAGC 1 cut(s) 252
HaeIII GGCC 1 cut(s) 130
HapII CCGG 2 cut(s) 479, 679
HgaI GACGC 5 cut(s) 637, 652, 668, 712, 721
Hin1I GRCGYC 6 cut(s) 629, 644, 704, 713, 731, 747
Hin1II CATG 4 cut(s) 287, 341, 420, 424
HincII GTYRAC 2 cut(s) 297, 735
HindII GTYRAC 2 cut(s) 297, 735
HinfI GANTC 3 cut(s) 145, 597, 696
HpaII CCGG 2 cut(s) 479, 679
HphI GGTGA 3 cut(s) 149, 186, 451
Hpy166II GTNNAC 4 cut(s) 55, 194, 297, 735
Hpy188I TCNGA 1 cut(s) 106
Hpy188III TCNNGA 6 cut(s) 11, 85, 594, 601, 693, 700
Hpy8I GTNNAC 4 cut(s) 55, 194, 297, 735
HpyAV CCTTC 1 cut(s) 161
HpyCH4III ACNGT 1 cut(s) 463
HpyCH4IV ACGT 3 cut(s) 405, 731, 747
HpyCH4V TGCA 8 cut(s) 23, 223, 341, 412, 420, 431, 476, 557
HpyF10VI GCNNNNNNNGC 3 cut(s) 377, 712, 721
HpyF3I CTNAG 2 cut(s) 198, 362
HpySE526I ACGT 3 cut(s) 405, 731, 747
Hsp92I GRCGYC 6 cut(s) 629, 644, 704, 713, 731, 747
Hsp92II CATG 4 cut(s) 287, 341, 420, 424
LmnI GCTCC 1 cut(s) 682
LpnPI CCDG 6 cut(s) 185, 262, 302, 483, 492, 692
Lsp1109I GCAGC 2 cut(s) 389, 460
LweI GCATC 2 cut(s) 232, 418
MaeI CTAG 3 cut(s) 273, 507, 576
MaeII ACGT 3 cut(s) 405, 731, 747
MaeIII GTNAC 2 cut(s) 184, 598
MboII GAAGA 6 cut(s) 73, 267, 619, 628, 634, 652
MhlI GDGCHC 2 cut(s) 570, 687
MluCI AATT 2 cut(s) 171, 495
MlyI GAGTC 2 cut(s) 154, 606
MmeI TCCRAC 1 cut(s) 95
MnlI CCTC 9 cut(s) 106, 207, 310, 542, 590, 593, 601, 678, 685
Mph1103I ATGCAT 2 cut(s) 422, 433
MroXI GAANNNNTTC 1 cut(s) 453
MslI CAYNNNNRTG 2 cut(s) 62, 119
MspA1I CMGCKG 1 cut(s) 380
MspI CCGG 2 cut(s) 479, 679
MspR9I CCNGG 2 cut(s) 679, 680
MwoI GCNNNNNNNGC 3 cut(s) 377, 712, 721
NciI CCSGG 2 cut(s) 679, 680
NheI GCTAGC 1 cut(s) 575
NlaIII CATG 4 cut(s) 287, 341, 420, 424
NlaIV GGNNCC 1 cut(s) 589
NmuCI GTSAC 2 cut(s) 184, 598
NsiI ATGCAT 2 cut(s) 422, 433
NspI RCATGY 1 cut(s) 420
OliI CACNNNNGTG 1 cut(s) 62
PaeI GCATGC 1 cut(s) 420
PcsI WCGNNNNNNNCGW 9 cut(s) 608, 611, 617, 632, 635, 644, 707, 728, 734
PdmI GAANNNNTTC 1 cut(s) 453
PfeI GAWTC 1 cut(s) 696
PflFI GACNNNGTC 2 cut(s) 730, 746
PkrI GCNGC 2 cut(s) 379, 475
PleI GAGTC 2 cut(s) 153, 605
PpsI GAGTC 2 cut(s) 153, 605
Psp124BI GAGCTC 1 cut(s) 687
PspFI CCCAGC 1 cut(s) 248
PspN4I GGNNCC 1 cut(s) 589
PspPI GGNCC 3 cut(s) 128, 587, 672
PsyI GACNNNGTC 2 cut(s) 730, 746
PvuII CAGCTG 1 cut(s) 380
RsaI GTAC 4 cut(s) 47, 54, 460, 465
RsaNI GTAC 4 cut(s) 46, 53, 459, 464
RseI CAYNNNNRTG 2 cut(s) 62, 119
SacI GAGCTC 1 cut(s) 687
SalI GTCGAC 2 cut(s) 295, 733
SatI GCNGC 2 cut(s) 378, 474
Sau96I GGNCC 3 cut(s) 128, 587, 672
ScaI AGTACT 1 cut(s) 465
SchI GAGTC 2 cut(s) 154, 606
ScrFI CCNGG 2 cut(s) 679, 680
SduI GDGCHC 2 cut(s) 570, 687
SfaNI GCATC 2 cut(s) 232, 418
SgrDI CGTCGACG 1 cut(s) 733
SinI GGWCC 2 cut(s) 587, 672
SmaI CCCGGG 1 cut(s) 680
SmiMI CAYNNNNRTG 2 cut(s) 62, 119
SmlI CTYRAG 1 cut(s) 237
SmoI CTYRAG 1 cut(s) 237
SphI GCATGC 1 cut(s) 420
Sse9I AATT 2 cut(s) 171, 495
SsiI CCGC 2 cut(s) 38, 326
SspI AATATT 1 cut(s) 267
SspMI CTAG 3 cut(s) 273, 507, 576
SstI GAGCTC 1 cut(s) 687
StyD4I CCNGG 2 cut(s) 677, 678
TaaI ACNGT 1 cut(s) 463
TaiI ACGT 3 cut(s) 408, 734, 750
TaqI TCGA 6 cut(s) 12, 84, 296, 694, 734, 750
TaqII GACCGA 4 cut(s) 575, 660, 668, 755
TasI AATT 2 cut(s) 171, 495
TatI WGTACW 3 cut(s) 45, 52, 463
TfiI GAWTC 1 cut(s) 696
TseFI GTSAC 2 cut(s) 184, 598
TseI GCWGC 2 cut(s) 377, 473
Tsp45I GTSAC 2 cut(s) 184, 598
TspDTI ATGAA 4 cut(s) 169, 326, 382, 437
TspMI CCCGGG 1 cut(s) 678
Tth111I GACNNNGTC 2 cut(s) 730, 746
VpaK11BI GGWCC 2 cut(s) 587, 672
XapI RAATTY 1 cut(s) 171
XceI RCATGY 1 cut(s) 420
XmaI CCCGGG 1 cut(s) 678
XmiI GTMKAC 2 cut(s) 296, 734
XmnI GAANNNNTTC 1 cut(s) 453
XspI CTAG 3 cut(s) 273, 507, 576
ZraI GACGTC 2 cut(s) 732, 748
ZrmI AGTACT 1 cut(s) 465
Zsp2I ATGCAT 2 cut(s) 422, 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.