FvH4_3g28240

O-acyltransferase (WSD1-like)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000634
Physical Location & Seq
Reverse (-)
21206711 .. 21209747
3037 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g28240.t1

Sequence Viewer

Length: 1185 bp
ATGGAGTTTGGCGAAGAAGGACTACTACAAGAGCCGGTGAGCCCCGGCTCTCAATTCATCAATAGCTCTGTCTTATCTCTTTCAATTCTTGCCGTTTTGGAATTTCAAGTTCCCATCGACGATTTACAAACCATTTCACTTCTGAAAAATGTCTTCCTGCCCATCAATCCACGCTTCTCTTCCATCATGGTTGAAGGTGTAGATAAACAGAATAAGCAGTGGAAGAGGGTCACAGTGAAGCTGGAAGACCATGTACGTGCCCCCATTTTCCCCGCTGGAATGTCACTTGAATCATATGACACTTGCTTTAACGAGTACATGACGCAGATAGCATCAGAAACATTCCCACAAAGCAAGCCATTATGGGAACTTCATCTTTTCCAGTACCCAACAAGTCACGCAGCTGGACAAATAATATTCAAGATCCACCATGCTCTTGGCGACGGCTACTCTCTCATGGGAGCTCTTCTCTCTCGTCTCCAAAATGCTCAAAATCCATCTCTTCCCTTGACATTTCCTTCATTAAAGGGTGCAAACAATGAAGCTACTAGTCGATCTCGTGTGTTTGAGTTTGTGCCTAAGATATTTTCTGCCGTTTTCAACAGTGCGTGGGATTTCAGTTGGGGCATTTTGAAGAGCACTTGGGTTGAAGATGACCGAACACCAATAAGGTCCGGCGTCGATGGAGTTGAGTTTCGGCCATTGTCCGTGTCCACCTTGATTCTGTCGATTGAGGAAATTAAACTTGTCAAGAGCAATCTCGGAGTGACGATAAATGATGTGATTACAGGAGCAATCTTCCTGGGCCTTCGAATGTACATGCAAAAGATGAATAGTGGAAAATCAAGCAGCGAGAATTGCACGGCACTGGTGTTGCTGAATACTAGACTTACTGCGGGTTACAAGTCAGTGCAGGATATGTTGAACGAAACAAACAAGTCTTGGGGAAACCGATTTGTATTCTTGCACGTCTCTGTGCCCAAGTCCAGTCAAGTTTCAAAGCCGCTGGATTTTGTGTGGGAAGCACAGCGTATAATCAAGAGGCAGAGAAGCTCTTCAGCTTGGTATCTCACCACTAGGCTTTGCGACATTTTGAAGAAACTTAGAGGCCCTGAGGTATTTTACTATATAGAATTACTAATTATTCTTAGCGGTTTATTTATTCCTCCATGTTTCATTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1298 WES_acyltransf

Protein Analysis

395

Amino Acids

44.42

Weight (kDa)

8.24

Isoelectric Point (pI)

46.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WS_DGAT_cat PF03007 62 - 262 8e-10 Wax ester synthase/diacylglycerol acyltransferase catalytic domain
WS_DGAT_C PF06974 315 - 374 2.5e-06 WS/DGAT C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000634)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10290 FvH4_3g28230 FvH4_3g28240
malus_domestica MD05G1036400.v1.1 MD10G1159400.v1.1 MD17G1237100.v1.1 MD17G1237200.v1.1 MD17G1237300.v1.1 MD17G1237600.v1.1 MD17G1238200.v1.1
prunus_persica Prupe.3G140400_v2.0.a1 Prupe.3G140700_v2.0.a1 Prupe.3G141100_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G258100_v2.0.a1 Prupe.7G117300_v2.0.a1 Prupe.7G117400_v2.0.a1 Prupe.7G117600_v2.0.a1 Prupe.8G044700_v2.0.a1
pyrus_communis pycom05g02680 pycom17g24220 pycom17g24240
rosa_chinensis RchiOBHm_Chr1g0350141 RchiOBHm_Chr5g0050931 RchiOBHm_Chr5g0051001 RchiOBHm_Chr5g0051021 RchiOBHm_Chr6g0267491
rosa_laevigata RLG00000013999 RLG00000028502 RLG00000034744 RLG00000034748 RLG00000034753
rosa_multiflora Rmu_sc0000446.1_g000056 Rmu_sc0000782.1_g000006 Rmu_sc0002951.1_g000001 Rmu_sc0003674.1_g000026 Rmu_sc0003756.1_g000004 Rmu_sc0011917.1_g000004 Rmu_sc0012005.1_g000019 Rmu_sc0040739.1_g000001
rosa_roxburghii Rroxscaffold_1G00029730 Rroxscaffold_1G00029740 Rroxscaffold_4G00305060 Rroxscaffold_7G00200710
rosa_rugosa Rorug01G0208400 Rorug05G0265200.1 Rorug05G0265300.1 Rorug05G0265400.1 Rorug05G0265500.1 Rorug05G0294000 Rorug06G0033100 Rorug06G0243300
rosa_samantha Rh1BG192100 Rh1BG192200 Rh1DG220600 Rh5AG270300 Rh5AG337200 Rh5AG337700 Rh5BG346700 Rh5BG347400 Rh5CG374300 Rh5DG359700 Rh5DG360500 Rh6AG154500 Rh6BG155100 Rh6BG362300 Rh6CG150300 Rh6DG140300 Rh6DG356300
rosa_wichuraiana Rw0G018820 Rw1G019350 Rw5G025300 Rw5G031770 Rw5G031820 Rw5G031870 Rw5G031910 Rw6G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 273, 896, 1004, 1152
AclWI GGATC 1 cut(s) 418
AcoI YGGCCR 1 cut(s) 698
AcsI RAATTY 1 cut(s) 101
AcuI CTGAAG 1 cut(s) 1041
AcyI GRCGYC 1 cut(s) 678
AfaI GTAC 4 cut(s) 255, 317, 386, 818
AhlI ACTAGT 1 cut(s) 548
AjiI CACGTC 1 cut(s) 970
AjnI CCWGG 1 cut(s) 801
AluBI AGCT 7 cut(s) 66, 241, 404, 464, 545, 1053, 1061
AluI AGCT 7 cut(s) 66, 241, 404, 464, 545, 1053, 1061
Alw21I GWGCWC 2 cut(s) 466, 641
Alw26I GTCTC 2 cut(s) 482, 976
AlwI GGATC 1 cut(s) 418
AoxI GGCC 3 cut(s) 698, 805, 1108
ApeKI GCWGC 2 cut(s) 401, 849
ApoI RAATTY 1 cut(s) 101
Asp700I GAANNNNTTC 1 cut(s) 1054
AspS9I GGNCC 3 cut(s) 672, 805, 1109
AsuC2I CCSGG 1 cut(s) 45
AsuHPI GGTGA 2 cut(s) 49, 1063
AsuII TTCGAA 1 cut(s) 811
AvaII GGWCC 1 cut(s) 672
AxyI CCTNAGG 1 cut(s) 1113
BaeGI GKGCMC 2 cut(s) 262, 981
BanII GRGCYC 2 cut(s) 44, 466
BarI GAAGNNNNNNTAC 5 cut(s) 9, 38, 41, 237, 269
BauI CACGAG 1 cut(s) 558
BbsI GAAGAC 2 cut(s) 145, 252
Bbv12I GWGCWC 2 cut(s) 466, 641
BbvI GCAGC 2 cut(s) 413, 861
BccI CCATC 5 cut(s) 122, 170, 191, 505, 677
BceAI ACGGC 4 cut(s) 77, 460, 578, 879
BciT130I CCWGG 1 cut(s) 803
BcnI CCSGG 1 cut(s) 45
BcoDI GTCTC 2 cut(s) 482, 976
BcuI ACTAGT 1 cut(s) 548
BfaI CTAG 3 cut(s) 549, 885, 1077
BisI GCNGC 3 cut(s) 402, 850, 1004
BlsI GCNGC 3 cut(s) 403, 851, 1005
Bme1390I CCNGG 2 cut(s) 45, 803
Bme18I GGWCC 1 cut(s) 672
BmgBI CACGTC 1 cut(s) 970
BmgT120I GGNCC 3 cut(s) 672, 805, 1109
BmrFI CCNGG 2 cut(s) 45, 803
BmsI GCATC 1 cut(s) 341
BpiI GAAGAC 2 cut(s) 145, 252
BplI GAGNNNNNCTC 2 cut(s) 453, 485
Bpu14I TTCGAA 1 cut(s) 811
BpuMI CCSGG 1 cut(s) 45
BsaAI YACGTR 1 cut(s) 257
BsaHI GRCGYC 1 cut(s) 678
BsaJI CCNNGG 2 cut(s) 43, 802
Bse118I RCCGGY 1 cut(s) 34
Bse1I ACTGG 3 cut(s) 382, 873, 987
Bse21I CCTNAGG 1 cut(s) 1113
BseBI CCWGG 1 cut(s) 803
BseDI CCNNGG 2 cut(s) 43, 802
BseMII CTCAG 1 cut(s) 1104
BseNI ACTGG 3 cut(s) 382, 873, 987
BseSI GKGCMC 2 cut(s) 262, 981
BseXI GCAGC 2 cut(s) 413, 861
BsgI GTGCAG 1 cut(s) 932
BshFI GGCC 3 cut(s) 700, 807, 1110
BsiHKAI GWGCWC 2 cut(s) 466, 641
BsiSI CCGG 3 cut(s) 35, 45, 675
BsmAI GTCTC 2 cut(s) 482, 976
BsmBI CGTCTC 2 cut(s) 482, 976
BsnI GGCC 3 cut(s) 700, 807, 1110
Bsp119I TTCGAA 1 cut(s) 811
Bsp1286I GDGCHC 5 cut(s) 44, 262, 466, 641, 981
Bsp1407I TGTACA 1 cut(s) 816
Bsp143I GATC 2 cut(s) 423, 554
BspACI CCGC 4 cut(s) 273, 896, 1004, 1152
BspANI GGCC 3 cut(s) 700, 807, 1110
BspCNI CTCAG 1 cut(s) 1105
BspPI GGATC 1 cut(s) 418
BspQI GCTCTTC 3 cut(s) 471, 629, 1060
BspT104I TTCGAA 1 cut(s) 811
BsrFI RCCGGY 1 cut(s) 34
BsrGI TGTACA 1 cut(s) 816
BsrI ACTGG 3 cut(s) 382, 873, 987
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 2 cut(s) 43, 802
BssMI GATC 2 cut(s) 423, 554
BssNI GRCGYC 1 cut(s) 678
BssSI CACGAG 1 cut(s) 558
Bst2BI CACGAG 1 cut(s) 558
Bst2UI CCWGG 1 cut(s) 803
Bst4CI ACNGT 2 cut(s) 235, 605
Bst6I CTCTTC 6 cut(s) 184, 218, 471, 507, 629, 1060
BstACI GRCGYC 1 cut(s) 678
BstAUI TGTACA 1 cut(s) 816
BstBAI YACGTR 1 cut(s) 257
BstBI TTCGAA 1 cut(s) 811
BstC8I GCNNGC 1 cut(s) 356
BstDEI CTNAG 4 cut(s) 579, 1103, 1113, 1148
BstKTI GATC 2 cut(s) 426, 557
BstMAI GTCTC 2 cut(s) 482, 976
BstMBI GATC 2 cut(s) 423, 554
BstMWI GCNNNNNNNGC 1 cut(s) 858
BstNI CCWGG 1 cut(s) 803
BstNSI RCATGY 1 cut(s) 823
BstSCI CCNGG 2 cut(s) 43, 801
BstSLI GKGCMC 2 cut(s) 262, 981
BstV1I GCAGC 2 cut(s) 413, 861
BstV2I GAAGAC 2 cut(s) 145, 252
BstX2I RGATCY 1 cut(s) 423
BstXI CCANNNNNNTGG 1 cut(s) 437
BstYI RGATCY 1 cut(s) 423
Bsu36I CCTNAGG 1 cut(s) 1113
BsuRI GGCC 3 cut(s) 700, 807, 1110
BtrI CACGTC 1 cut(s) 970
BtsI GCAGTG 1 cut(s) 224
BtsIMutI CAGTG 6 cut(s) 224, 240, 610, 866, 915, 1180
Cac8I GCNNGC 1 cut(s) 356
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 3 cut(s) 672, 805, 1109
CseI GACGC 2 cut(s) 331, 667
Csp6I GTAC 4 cut(s) 254, 316, 385, 817
CviAII CATG 7 cut(s) 187, 251, 319, 431, 457, 820, 1170
CviQI GTAC 4 cut(s) 254, 316, 385, 817
DdeI CTNAG 4 cut(s) 579, 1103, 1113, 1148
DpnI GATC 2 cut(s) 425, 556
DpnII GATC 2 cut(s) 423, 554
EaeI YGGCCR 1 cut(s) 698
Eam1104I CTCTTC 6 cut(s) 184, 218, 471, 507, 629, 1060
EarI CTCTTC 6 cut(s) 184, 218, 471, 507, 629, 1060
Ecl136II GAGCTC 1 cut(s) 464
Eco24I GRGCYC 2 cut(s) 44, 466
Eco47I GGWCC 1 cut(s) 672
Eco53kI GAGCTC 1 cut(s) 464
Eco57I CTGAAG 1 cut(s) 1041
Eco81I CCTNAGG 1 cut(s) 1113
EcoICRI GAGCTC 1 cut(s) 464
EcoO109I RGGNCCY 1 cut(s) 1109
EcoRII CCWGG 1 cut(s) 801
EcoT38I GRGCYC 2 cut(s) 44, 466
Esp3I CGTCTC 2 cut(s) 482, 976
FaeI CATG 7 cut(s) 190, 254, 322, 434, 460, 823, 1173
FatI CATG 7 cut(s) 186, 250, 318, 430, 456, 819, 1169
FauI CCCGC 2 cut(s) 280, 889
FauNDI CATATG 1 cut(s) 295
Fnu4HI GCNGC 3 cut(s) 402, 850, 1004
FriOI GRGCYC 2 cut(s) 44, 466
Fsp4HI GCNGC 3 cut(s) 402, 850, 1004
FspBI CTAG 3 cut(s) 549, 885, 1077
GluI GCNGC 3 cut(s) 402, 850, 1004
HaeIII GGCC 3 cut(s) 700, 807, 1110
HapII CCGG 3 cut(s) 35, 45, 675
HgaI GACGC 2 cut(s) 331, 667
Hin1I GRCGYC 1 cut(s) 678
Hin1II CATG 7 cut(s) 190, 254, 322, 434, 460, 823, 1173
HinfI GANTC 2 cut(s) 290, 721
HpaII CCGG 3 cut(s) 35, 45, 675
HphI GGTGA 2 cut(s) 49, 1063
Hpy166II GTNNAC 1 cut(s) 714
Hpy188I TCNGA 3 cut(s) 144, 337, 764
Hpy188III TCNNGA 3 cut(s) 421, 751, 1039
Hpy8I GTNNAC 1 cut(s) 714
Hpy99I CGWCG 3 cut(s) 122, 446, 683
HpyAV CCTTC 4 cut(s) 11, 188, 528, 818
HpyCH4III ACNGT 2 cut(s) 235, 605
HpyCH4IV ACGT 2 cut(s) 256, 969
HpyCH4V TGCA 5 cut(s) 533, 823, 861, 913, 967
HpyF10VI GCNNNNNNNGC 1 cut(s) 858
HpyF3I CTNAG 4 cut(s) 579, 1103, 1113, 1148
HpySE526I ACGT 2 cut(s) 256, 969
Hsp92I GRCGYC 1 cut(s) 678
Hsp92II CATG 7 cut(s) 190, 254, 322, 434, 460, 823, 1173
Kzo9I GATC 2 cut(s) 423, 554
LguI GCTCTTC 3 cut(s) 471, 629, 1060
LmnI GCTCC 2 cut(s) 461, 791
Lsp1109I GCAGC 2 cut(s) 413, 861
LweI GCATC 1 cut(s) 341
MaeI CTAG 3 cut(s) 549, 885, 1077
MaeII ACGT 2 cut(s) 256, 969
MaeIII GTNAC 5 cut(s) 229, 282, 395, 766, 899
MalI GATC 2 cut(s) 425, 556
MboI GATC 2 cut(s) 423, 554
MflI RGATCY 1 cut(s) 423
MhlI GDGCHC 5 cut(s) 44, 262, 466, 641, 981
MluCI AATT 7 cut(s) 53, 84, 101, 738, 856, 1133, 1140
MnlI CCTC 6 cut(s) 219, 727, 1035, 1100, 1108, 1176
MroXI GAANNNNTTC 1 cut(s) 1054
MseI TTAA 3 cut(s) 309, 524, 741
MslI CAYNNNNRTG 1 cut(s) 255
MspA1I CMGCKG 3 cut(s) 275, 404, 1006
MspI CCGG 3 cut(s) 35, 45, 675
MspR9I CCNGG 2 cut(s) 45, 803
MvaI CCWGG 1 cut(s) 803
MwoI GCNNNNNNNGC 1 cut(s) 858
NciI CCSGG 1 cut(s) 45
NdeI CATATG 1 cut(s) 295
NdeII GATC 2 cut(s) 423, 554
NlaIII CATG 7 cut(s) 190, 254, 322, 434, 460, 823, 1173
NmuCI GTSAC 4 cut(s) 229, 282, 395, 766
NspI RCATGY 1 cut(s) 823
NspV TTCGAA 1 cut(s) 811
PciSI GCTCTTC 3 cut(s) 471, 629, 1060
PdmI GAANNNNTTC 1 cut(s) 1054
PfeI GAWTC 2 cut(s) 290, 721
PkrI GCNGC 3 cut(s) 403, 851, 1005
Ppu21I YACGTR 1 cut(s) 257
Psp124BI GAGCTC 1 cut(s) 466
Psp6I CCWGG 1 cut(s) 801
PspGI CCWGG 1 cut(s) 801
PspPI GGNCC 3 cut(s) 672, 805, 1109
PsuI RGATCY 1 cut(s) 423
PvuII CAGCTG 1 cut(s) 404
RsaI GTAC 4 cut(s) 255, 317, 386, 818
RsaNI GTAC 4 cut(s) 254, 316, 385, 817
RseI CAYNNNNRTG 1 cut(s) 255
SacI GAGCTC 1 cut(s) 466
SapI GCTCTTC 3 cut(s) 471, 629, 1060
SaqAI TTAA 3 cut(s) 309, 524, 741
SatI GCNGC 3 cut(s) 402, 850, 1004
Sau3AI GATC 2 cut(s) 423, 554
Sau96I GGNCC 3 cut(s) 672, 805, 1109
ScrFI CCNGG 2 cut(s) 45, 803
SduI GDGCHC 5 cut(s) 44, 262, 466, 641, 981
SfaNI GCATC 1 cut(s) 341
SfuI TTCGAA 1 cut(s) 811
SinI GGWCC 1 cut(s) 672
SmiMI CAYNNNNRTG 1 cut(s) 255
SpeI ACTAGT 1 cut(s) 548
Sse9I AATT 7 cut(s) 53, 84, 101, 738, 856, 1133, 1140
SsiI CCGC 4 cut(s) 273, 896, 1004, 1152
SspI AATATT 1 cut(s) 417
SspMI CTAG 3 cut(s) 549, 885, 1077
SstI GAGCTC 1 cut(s) 466
StyD4I CCNGG 2 cut(s) 43, 801
TaaI ACNGT 2 cut(s) 235, 605
TaiI ACGT 2 cut(s) 259, 972
TaqI TCGA 5 cut(s) 117, 553, 681, 728, 811
TaqII GACCGA 1 cut(s) 672
TasI AATT 7 cut(s) 53, 84, 101, 738, 856, 1133, 1140
TatI WGTACW 2 cut(s) 315, 816
TauI GCSGC 1 cut(s) 1006
TfiI GAWTC 2 cut(s) 290, 721
Tru1I TTAA 3 cut(s) 309, 524, 741
Tru9I TTAA 3 cut(s) 309, 524, 741
TscAI CASTG 5 cut(s) 224, 240, 610, 873, 915
TseFI GTSAC 4 cut(s) 229, 282, 395, 766
TseI GCWGC 2 cut(s) 401, 849
Tsp45I GTSAC 4 cut(s) 229, 282, 395, 766
TspDTI ATGAA 6 cut(s) 46, 362, 510, 555, 845, 1165
TspGWI ACGGA 1 cut(s) 697
TspRI CASTG 5 cut(s) 224, 240, 610, 873, 915
VpaK11BI GGWCC 1 cut(s) 672
XapI RAATTY 1 cut(s) 101
XceI RCATGY 1 cut(s) 823
XcmI CCANNNNNNNNNTGG 1 cut(s) 434
XmnI GAANNNNTTC 1 cut(s) 1054
XspI CTAG 3 cut(s) 549, 885, 1077
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.