Rroxscaffold_1G00029730

Wax ester synthase-like Acyl-CoA acyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000634
Physical Location & Seq
Forward (+)
38580350 .. 38582755
2406 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00029730.1

Sequence Viewer

Length: 1062 bp
ATGTCCCCATTTTCCCCTCTGGAATGTCCCCCTGCATCATACGATATTTACTTTGATGAGTACATAACGAAGATAGCATCAGAAACATTACCTCAAAGCAGGCCATTATGGGAACTTCATCTTTTCAAGTACCCAACAAGTCATGCAGCTGGACAAATGATATTCAAGATCCACCATGCCCTTGGCGATGGCTACTCTCTCATGGGCGCTCTTCTCTCTTGCCTCCAGAATGCTCACAATCCTTCCATTCCTCTGACATTTCCGACACTAAAGAGTGAAAAAAATGAAACTAGCAGTCGATCTCGTGCGTTTGAGATTATACCTAAGATACTTTCTTCCGTTTTCAACGGTGCATGGGATTTCAGTTGGAGCATTTTGAAGGGCACTTGGGTTGAAGATGATCGAACACCAATAAGGTCCGGTGTTGAAGGAGTTGAGTTTCGACCTGTGTCGGTGTCAACCTTGATGCTGCCTATTGAGGAAATTAAACTTATCAAGAACAAGCTTGGAGTGACGATAAATGATGTGATTTCAGGAACAATCTTTATGGGCATCCGAATGTACATTCAAGAGATGAATAGTGAAAAATCAAGTAGCCAAAATTGCACAGCACTGGTGTTGCTGAATACTAGACTTGTTGCGGGTTACAAGTCAGTGCAGGAGATGTTGATCGAAACAAACAAGTCTTGGGGAAACCGATTTGTGTTCTTGCAAGTCTCGGTGCCCAAGTCGAGTGAAGTTTCAAAGCCACTGGATTTTGTGTGGGAAGCACATAATATAATCAAGAGACAGAGAAACTCTTCATCTTGGTACCTCACCACTAGGCTCTGGGATATTTTGAAGAAATTTAGAGGCCCTGAGAATGAGAACTCAAAATATCACCCAAGTCCTCATAAAGACGTCCAAGCAACGACCAGTTGCTTCCATTATGCCTACAGTGCTGTTGTTGCACAAGTTGAGAATCTATTTCTACTATACCTGGAACCAAGTTTTGATCCAATGCAAAAGCAAAGGCAAATTCACAAGCCAGAGCTTCAGCCTGTTCCACCGAAAATAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1298 WES_acyltransf

Protein Analysis

353

Amino Acids

40.26

Weight (kDa)

8.51

Isoelectric Point (pI)

57.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WS_DGAT_cat PF03007 18 - 177 2.8e-11 Wax ester synthase/diacylglycerol acyltransferase catalytic domain
WS_DGAT_C PF06974 230 - 292 4.8e-06 WS/DGAT C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000634)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10290 FvH4_3g28230 FvH4_3g28240
malus_domestica MD05G1036400.v1.1 MD10G1159400.v1.1 MD17G1237100.v1.1 MD17G1237200.v1.1 MD17G1237300.v1.1 MD17G1237600.v1.1 MD17G1238200.v1.1
prunus_persica Prupe.3G140400_v2.0.a1 Prupe.3G140700_v2.0.a1 Prupe.3G141100_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G257800_v2.0.a1 Prupe.4G258100_v2.0.a1 Prupe.7G117300_v2.0.a1 Prupe.7G117400_v2.0.a1 Prupe.7G117600_v2.0.a1 Prupe.8G044700_v2.0.a1
pyrus_communis pycom05g02680 pycom17g24220 pycom17g24240
rosa_chinensis RchiOBHm_Chr1g0350141 RchiOBHm_Chr5g0050931 RchiOBHm_Chr5g0051001 RchiOBHm_Chr5g0051021 RchiOBHm_Chr6g0267491
rosa_laevigata RLG00000013999 RLG00000028502 RLG00000034744 RLG00000034748 RLG00000034753
rosa_multiflora Rmu_sc0000446.1_g000056 Rmu_sc0000782.1_g000006 Rmu_sc0002951.1_g000001 Rmu_sc0003674.1_g000026 Rmu_sc0003756.1_g000004 Rmu_sc0011917.1_g000004 Rmu_sc0012005.1_g000019 Rmu_sc0040739.1_g000001
rosa_roxburghii Rroxscaffold_1G00029730 Rroxscaffold_1G00029740 Rroxscaffold_4G00305060 Rroxscaffold_7G00200710
rosa_rugosa Rorug01G0208400 Rorug05G0265200.1 Rorug05G0265300.1 Rorug05G0265400.1 Rorug05G0265500.1 Rorug05G0294000 Rorug06G0033100 Rorug06G0243300
rosa_samantha Rh1BG192100 Rh1BG192200 Rh1DG220600 Rh5AG270300 Rh5AG337200 Rh5AG337700 Rh5BG346700 Rh5BG347400 Rh5CG374300 Rh5DG359700 Rh5DG360500 Rh6AG154500 Rh6BG155100 Rh6BG362300 Rh6CG150300 Rh6DG140300 Rh6DG356300
rosa_wichuraiana Rw0G018820 Rw1G019350 Rw5G025300 Rw5G031770 Rw5G031820 Rw5G031870 Rw5G031910 Rw6G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 903
Acc65I GGTACC 1 cut(s) 810
AccB1I GGYRCC 2 cut(s) 721, 810
AciI CCGC 1 cut(s) 641
AclWI GGATC 2 cut(s) 163, 989
AcsI RAATTY 2 cut(s) 845, 1017
AcuI CTGAAG 1 cut(s) 1019
AcyI GRCGYC 1 cut(s) 900
AfaI GTAC 4 cut(s) 62, 131, 563, 812
AgsI TTSAA 9 cut(s) 127, 166, 346, 379, 395, 428, 569, 744, 841
AjnI CCWGG 1 cut(s) 978
AluBI AGCT 3 cut(s) 149, 505, 1033
AluI AGCT 3 cut(s) 149, 505, 1033
Alw26I GTCTC 2 cut(s) 721, 781
AlwI GGATC 2 cut(s) 163, 989
AoxI GGCC 2 cut(s) 101, 853
ApeKI GCWGC 2 cut(s) 146, 469
ApoI RAATTY 2 cut(s) 845, 1017
Asp700I GAANNNNTTC 1 cut(s) 799
Asp718I GGTACC 1 cut(s) 810
AspLEI GCGC 1 cut(s) 209
AspS9I GGNCC 2 cut(s) 417, 854
AsuHPI GGTGA 2 cut(s) 808, 872
AvaII GGWCC 1 cut(s) 417
BaeGI GKGCMC 2 cut(s) 386, 726
BanI GGYRCC 2 cut(s) 721, 810
BauI CACGAG 1 cut(s) 303
BbvI GCAGC 2 cut(s) 158, 456
BccI CCATC 1 cut(s) 182
BciT130I CCWGG 1 cut(s) 980
BcoDI GTCTC 2 cut(s) 721, 781
BfaI CTAG 4 cut(s) 291, 630, 822, 1060
BfmI CTRYAG 1 cut(s) 934
BfoI RGCGCY 1 cut(s) 210
BisI GCNGC 2 cut(s) 147, 470
BlsI GCNGC 2 cut(s) 148, 471
Bme1390I CCNGG 1 cut(s) 980
Bme18I GGWCC 1 cut(s) 417
BmgT120I GGNCC 2 cut(s) 417, 854
BmiI GGNNCC 3 cut(s) 723, 812, 984
BmrFI CCNGG 1 cut(s) 980
BmsI GCATC 4 cut(s) 44, 86, 456, 561
BoxI GACNNNNGTC 1 cut(s) 448
BpmI CTGGAG 1 cut(s) 209
BsaBI GATNNNNATC 1 cut(s) 668
BsaHI GRCGYC 1 cut(s) 900
BsaJI CCNNGG 1 cut(s) 181
BsaWI WCCGGW 1 cut(s) 419
Bse1I ACTGG 3 cut(s) 618, 756, 915
Bse8I GATNNNNATC 1 cut(s) 668
BseBI CCWGG 1 cut(s) 980
BseDI CCNNGG 1 cut(s) 181
BseGI GGATG 1 cut(s) 552
BseJI GATNNNNATC 1 cut(s) 668
BseMII CTCAG 1 cut(s) 849
BseNI ACTGG 3 cut(s) 618, 756, 915
BseSI GKGCMC 2 cut(s) 386, 726
BseXI GCAGC 2 cut(s) 158, 456
BsgI GTGCAG 1 cut(s) 677
BshFI GGCC 2 cut(s) 103, 855
BshNI GGYRCC 2 cut(s) 721, 810
BsiSI CCGG 1 cut(s) 420
BslFI GGGAC 1 cut(s) 12
BsmAI GTCTC 2 cut(s) 721, 781
BsmFI GGGAC 1 cut(s) 12
BsmI GAATGC 1 cut(s) 235
BsnI GGCC 2 cut(s) 103, 855
Bsp1286I GDGCHC 2 cut(s) 386, 726
Bsp1407I TGTACA 1 cut(s) 561
Bsp143I GATC 5 cut(s) 168, 299, 400, 669, 994
BspACI CCGC 1 cut(s) 641
BspANI GGCC 2 cut(s) 103, 855
BspCNI CTCAG 1 cut(s) 850
BspLI GGNNCC 3 cut(s) 723, 812, 984
BspPI GGATC 2 cut(s) 163, 989
BspQI GCTCTTC 1 cut(s) 216
BspT107I GGYRCC 2 cut(s) 721, 810
BsrGI TGTACA 1 cut(s) 561
BsrI ACTGG 3 cut(s) 618, 756, 915
BssECI CCNNGG 1 cut(s) 181
BssMI GATC 5 cut(s) 168, 299, 400, 669, 994
BssNI GRCGYC 1 cut(s) 900
BssSI CACGAG 1 cut(s) 303
BssT1I CCWWGG 1 cut(s) 181
Bst2BI CACGAG 1 cut(s) 303
Bst2UI CCWGG 1 cut(s) 980
Bst4CI ACNGT 2 cut(s) 350, 938
Bst6I CTCTTC 2 cut(s) 216, 805
BstACI GRCGYC 1 cut(s) 900
BstAUI TGTACA 1 cut(s) 561
BstC8I GCNNGC 1 cut(s) 101
BstDEI CTNAG 2 cut(s) 324, 858
BstF5I GGATG 1 cut(s) 552
BstH2I RGCGCY 1 cut(s) 210
BstHHI GCGC 1 cut(s) 209
BstKTI GATC 5 cut(s) 171, 302, 403, 672, 997
BstMAI GTCTC 2 cut(s) 721, 781
BstMBI GATC 5 cut(s) 168, 299, 400, 669, 994
BstMWI GCNNNNNNNGC 3 cut(s) 603, 938, 947
BstNI CCWGG 1 cut(s) 980
BstPAI GACNNNNGTC 1 cut(s) 448
BstSCI CCNGG 1 cut(s) 978
BstSFI CTRYAG 1 cut(s) 934
BstSLI GKGCMC 2 cut(s) 386, 726
BstV1I GCAGC 2 cut(s) 158, 456
BstX2I RGATCY 1 cut(s) 168
BstXI CCANNNNNNTGG 1 cut(s) 182
BstYI RGATCY 1 cut(s) 168
BsuRI GGCC 2 cut(s) 103, 855
BtgZI GCGATG 1 cut(s) 201
BtsCI GGATG 1 cut(s) 552
BtsIMutI CAGTG 4 cut(s) 611, 660, 749, 943
Cac8I GCNNGC 1 cut(s) 101
CfoI GCGC 1 cut(s) 209
Cfr13I GGNCC 2 cut(s) 417, 854
Csp6I GTAC 4 cut(s) 61, 130, 562, 811
CviAII CATG 4 cut(s) 143, 176, 202, 354
CviQI GTAC 4 cut(s) 61, 130, 562, 811
DdeI CTNAG 2 cut(s) 324, 858
DpnI GATC 5 cut(s) 170, 301, 402, 671, 996
DpnII GATC 5 cut(s) 168, 299, 400, 669, 994
Eam1104I CTCTTC 2 cut(s) 216, 805
EarI CTCTTC 2 cut(s) 216, 805
Eco130I CCWWGG 1 cut(s) 181
Eco47I GGWCC 1 cut(s) 417
Eco57I CTGAAG 1 cut(s) 1019
EcoO109I RGGNCCY 1 cut(s) 854
EcoRII CCWGG 1 cut(s) 978
EcoT14I CCWWGG 1 cut(s) 181
ErhI CCWWGG 1 cut(s) 181
FaeI CATG 4 cut(s) 146, 179, 205, 357
FaqI GGGAC 1 cut(s) 12
FatI CATG 4 cut(s) 142, 175, 201, 353
FauI CCCGC 1 cut(s) 634
Fnu4HI GCNGC 2 cut(s) 147, 470
FokI GGATG 1 cut(s) 539
Fsp4HI GCNGC 2 cut(s) 147, 470
FspBI CTAG 4 cut(s) 291, 630, 822, 1060
GlaI GCGC 1 cut(s) 208
GluI GCNGC 2 cut(s) 147, 470
GsuI CTGGAG 1 cut(s) 209
HaeII RGCGCY 1 cut(s) 210
HaeIII GGCC 2 cut(s) 103, 855
HapII CCGG 1 cut(s) 420
HhaI GCGC 1 cut(s) 209
Hin1I GRCGYC 1 cut(s) 900
Hin1II CATG 4 cut(s) 146, 179, 205, 357
Hin6I GCGC 1 cut(s) 207
HinP1I GCGC 1 cut(s) 207
HincII GTYRAC 1 cut(s) 459
HindII GTYRAC 1 cut(s) 459
HindIII AAGCTT 1 cut(s) 503
HinfI GANTC 1 cut(s) 961
HpaII CCGG 1 cut(s) 420
HphI GGTGA 2 cut(s) 808, 872
Hpy166II GTNNAC 1 cut(s) 459
Hpy188I TCNGA 4 cut(s) 82, 255, 264, 557
Hpy188III TCNNGA 7 cut(s) 20, 166, 226, 496, 534, 569, 784
Hpy8I GTNNAC 1 cut(s) 459
HpyAV CCTTC 3 cut(s) 252, 373, 422
HpyCH4III ACNGT 2 cut(s) 350, 938
HpyCH4IV ACGT 1 cut(s) 900
HpyCH4V TGCA 8 cut(s) 35, 146, 353, 606, 658, 712, 950, 1003
HpyF10VI GCNNNNNNNGC 3 cut(s) 603, 938, 947
HpyF3I CTNAG 2 cut(s) 324, 858
HpySE526I ACGT 1 cut(s) 900
Hsp92I GRCGYC 1 cut(s) 900
Hsp92II CATG 4 cut(s) 146, 179, 205, 357
HspAI GCGC 1 cut(s) 207
KpnI GGTACC 1 cut(s) 814
Kzo9I GATC 5 cut(s) 168, 299, 400, 669, 994
LguI GCTCTTC 1 cut(s) 216
LmnI GCTCC 1 cut(s) 369
Lsp1109I GCAGC 2 cut(s) 158, 456
LweI GCATC 4 cut(s) 44, 86, 456, 561
MaeI CTAG 4 cut(s) 291, 630, 822, 1060
MaeII ACGT 1 cut(s) 900
MaeIII GTNAC 2 cut(s) 511, 644
MalI GATC 5 cut(s) 170, 301, 402, 671, 996
MboI GATC 5 cut(s) 168, 299, 400, 669, 994
MboII GAAGA 6 cut(s) 82, 203, 327, 407, 792, 853
MflI RGATCY 1 cut(s) 168
MhlI GDGCHC 2 cut(s) 386, 726
MluCI AATT 4 cut(s) 483, 601, 845, 1017
MmeI TCCRAC 2 cut(s) 287, 347
MnlI CCTC 8 cut(s) 27, 102, 233, 261, 472, 824, 845, 900
MroXI GAANNNNTTC 1 cut(s) 799
MseI TTAA 1 cut(s) 486
MslI CAYNNNNRTG 1 cut(s) 557
MspA1I CMGCKG 1 cut(s) 149
MspI CCGG 1 cut(s) 420
MspR9I CCNGG 1 cut(s) 980
Mva1269I GAATGC 1 cut(s) 235
MvaI CCWGG 1 cut(s) 980
MwoI GCNNNNNNNGC 3 cut(s) 603, 938, 947
NdeII GATC 5 cut(s) 168, 299, 400, 669, 994
NlaIII CATG 4 cut(s) 146, 179, 205, 357
NlaIV GGNNCC 3 cut(s) 723, 812, 984
NmuCI GTSAC 1 cut(s) 511
PciSI GCTCTTC 1 cut(s) 216
PctI GAATGC 1 cut(s) 235
PdmI GAANNNNTTC 1 cut(s) 799
PfeI GAWTC 1 cut(s) 961
PkrI GCNGC 2 cut(s) 148, 471
PshAI GACNNNNGTC 1 cut(s) 448
Psp6I CCWGG 1 cut(s) 978
PspGI CCWGG 1 cut(s) 978
PspN4I GGNNCC 3 cut(s) 723, 812, 984
PspPI GGNCC 2 cut(s) 417, 854
PsuI RGATCY 1 cut(s) 168
PvuII CAGCTG 1 cut(s) 149
RsaI GTAC 4 cut(s) 62, 131, 563, 812
RsaNI GTAC 4 cut(s) 61, 130, 562, 811
RseI CAYNNNNRTG 1 cut(s) 557
SapI GCTCTTC 1 cut(s) 216
SaqAI TTAA 1 cut(s) 486
SatI GCNGC 2 cut(s) 147, 470
Sau3AI GATC 5 cut(s) 168, 299, 400, 669, 994
Sau96I GGNCC 2 cut(s) 417, 854
ScrFI CCNGG 1 cut(s) 980
SduI GDGCHC 2 cut(s) 386, 726
SfaNI GCATC 4 cut(s) 44, 86, 456, 561
SfcI CTRYAG 1 cut(s) 934
SinI GGWCC 1 cut(s) 417
SmiMI CAYNNNNRTG 1 cut(s) 557
Sse9I AATT 4 cut(s) 483, 601, 845, 1017
SsiI CCGC 1 cut(s) 641
SspMI CTAG 4 cut(s) 291, 630, 822, 1060
StyD4I CCNGG 1 cut(s) 978
StyI CCWWGG 1 cut(s) 181
TaaI ACNGT 2 cut(s) 350, 938
TaiI ACGT 1 cut(s) 903
TaqI TCGA 5 cut(s) 298, 403, 442, 672, 731
TasI AATT 4 cut(s) 483, 601, 845, 1017
TatI WGTACW 2 cut(s) 60, 561
TfiI GAWTC 1 cut(s) 961
Tru1I TTAA 1 cut(s) 486
Tru9I TTAA 1 cut(s) 486
TscAI CASTG 4 cut(s) 618, 660, 756, 943
TseFI GTSAC 1 cut(s) 511
TseI GCWGC 2 cut(s) 146, 469
Tsp45I GTSAC 1 cut(s) 511
TspDTI ATGAA 4 cut(s) 107, 300, 590, 792
TspGWI ACGGA 1 cut(s) 328
TspRI CASTG 4 cut(s) 618, 660, 756, 943
VpaK11BI GGWCC 1 cut(s) 417
XapI RAATTY 2 cut(s) 845, 1017
XcmI CCANNNNNNNNNTGG 1 cut(s) 179
XmnI GAANNNNTTC 1 cut(s) 799
XspI CTAG 4 cut(s) 291, 630, 822, 1060
ZraI GACGTC 1 cut(s) 901
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.