FvH4_2g16190

Endochitinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000495
Physical Location & Seq
Reverse (-)
14149403 .. 14151409
2007 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g16190.t1

Sequence Viewer

Length: 1044 bp
ATGCTTGGCTTTCAAACTTCAAACCAAATCACAAACAAAATCAATACCATGATGTTGAACTTGGCCTTGTCAATCACTTCTTTTCTATTGCTCTTTAATATCCGAGGAGGCTCAGCAGAGCAATGTGGAAACCAAGCAGGGGGTGCTTTATGCCCGGGAGGGCAATGCTGCAGCAAGTTCGGCTGGTGTGGCACTACGCCTGATTACTGCAGCACCGATTGCCAAAGCCAATGCGGCGGCGGTGGTGGTGGAGGAGGCGGTGGTGACATTAAGGATCTAGTTTCAAAGGACAGCTTTGATAAGATACTCAAGCATAGGGACGACGGTGGTTGCCCTGCCAAAGGGTTTTATACTTATGATGCTTTCATTGCTGCTGCCAAAGCCTTCCCCGCCTTTGCTACAACTGGTGATACTGCCACCCGTAAGAGGGAGATTGCTGCTTTCTTAGCTCAAACTTCTCATGAAACTACTGGGGGATGGGCGACTGCACCAGATGGACCATACGCATGGGGCTATTGTTTTAACAAGGAGAAAAATCCAGGATCTTACTGTGCCTCGGATCCAAATTATCCATGTGCTGCTGGTAAGCAGTATTATGGCCGCGGTCCTATTCAACTTTCTTGGAATTACAACTATGGACAGTGTGGAAAAGCCATAGGAGCGAACTTGCTGAACAACCCGGACCTTGTTGCCACAGACCCTGTGATCTCCTTCAAGACAGCATTCTGGTTCTGGATGACCCCTCAATCACCCAAGCCGTCGTGCCATGATGTTATCACCGGGAAATGGAACCCTTCCGGAGCCGATAAGTCAGCCGGTAGGTCTTCCGGGTATGGTACGATCACAAACATCATCAACGGTGGACTTGAATGTGGTAAGGGCTCGGATGGGAGAGTGGCGGACCGCATAGGGTTTTTCAAGAGGTACTGTGATTTGCTTGGAGTTGGTTATGGTGACAACCTTGACTGCTATAATCAGAAGCCGTTTGGGTCTGGACTCGCTACTTATTATTCACCATCCTCATCAAAAGTGTCCTCGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 ko04016 map00520 map01100 map04016
Pfam Domains
Protein Families
Chitin_bind_1 Glyco_hydro_19

Protein Analysis

348

Amino Acids

36.71

Weight (kDa)

8.23

Isoelectric Point (pI)

33.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chitin_bind_1 PF00187 41 - 78 1.8e-13 Chitin recognition protein
Glyco_hydro_19 PF00182 93 - 323 1.1e-129 Chitinase class I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000495)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12500
fragaria_vesca FvH4_1g10640 FvH4_1g10640 FvH4_1g10640 FvH4_1g10640 FvH4_1g10640 FvH4_1g10641 FvH4_1g10641 FvH4_1g10642 FvH4_1g10643 FvH4_1g10650 FvH4_1g10660 FvH4_1g10660 FvH4_1g10661 FvH4_2g16190
malus_domestica MD02G1120200.v1.1 MD02G1120300.v1.1 MD15G1234400.v1.1
prunus_persica Prupe.7G178500_v2.0.a1 Prupe.8G174900_v2.0.a1
pyrus_communis pycom02g09370 pycom02g09380 pycom02g09390
rosa_chinensis RchiOBHm_Chr2g0097671 RchiOBHm_Chr2g0097691 RchiOBHm_Chr2g0097731 RchiOBHm_Chr2g0097781 RchiOBHm_Chr2g0097801 RchiOBHm_Chr2g0097821 RchiOBHm_Chr6g0279811 RchiOBHm_Chr6g0279831 RchiOBHm_Chr6g0279841
rosa_laevigata RLG00000013112 RLG00000013114 RLG00000016734 RLG00000016736 RLG00000016737 RLG00000016738
rosa_multiflora Rmu_sc0000974.1_g000004 Rmu_sc0000974.1_g000013 Rmu_sc0000974.1_g000019 Rmu_sc0000974.1_g000025 Rmu_sc0002340.1_g000010 Rmu_sc0008442.1_g000003 Rmu_sc0008442.1_g000009 Rmu_sc0020401.1_g000002
rosa_roxburghii Rroxscaffold_2G00140520 Rroxscaffold_2G00144540 Rroxscaffold_2G00144580 Rroxscaffold_2G00144590 Rroxscaffold_7G00188570 Rroxscaffold_7G00188590 Rroxscaffold_7G00188610
rosa_rugosa Rorug02G0068300 Rorug02G0068400 Rorug06G0128600
rosa_samantha Rh2AG114800 Rh2AG114900 Rh2AG115000 Rh2AG115100 Rh2BG117600 Rh2BG117800 Rh2BG117900 Rh2BG118000 Rh2CG119600 Rh2DG118500 Rh2DG118800 Rh2DG119100 Rh2DG119200 Rh2DG119300 Rh5AG369400 Rh5CG403900 Rh6AG236600 Rh6AG236700 Rh6AG236800 Rh6AG237300 Rh6BG241000 Rh6BG241200 Rh6BG241400 Rh6CG243300 Rh6CG243400 Rh6CG243600 Rh6DG234400 Rh6DG234500 Rh7AG282100
rosa_wichuraiana Rw2G008980 Rw2G008990 Rw6G020640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 603
AccIII TCCGGA 1 cut(s) 797
AciI CCGC 9 cut(s) 234, 237, 240, 258, 390, 601, 603, 899, 904
AclWI GGATC 4 cut(s) 282, 550, 554, 567
AcoI YGGCCR 1 cut(s) 598
AfaI GTAC 2 cut(s) 838, 926
AfiI CCNNNNNNNGG 5 cut(s) 139, 341, 426, 427, 786
AgsI TTSAA 8 cut(s) 14, 21, 58, 285, 614, 715, 869, 919
AjnI CCWGG 1 cut(s) 538
AluBI AGCT 2 cut(s) 294, 449
AluI AGCT 2 cut(s) 294, 449
AlwI GGATC 4 cut(s) 282, 550, 554, 567
AlwNI CAGNNNCTG 1 cut(s) 701
Ama87I CYCGRG 1 cut(s) 154
Aor13HI TCCGGA 1 cut(s) 797
AoxI GGCC 2 cut(s) 63, 598
ApeKI GCWGC 7 cut(s) 168, 171, 210, 371, 374, 437, 578
AspS9I GGNCC 4 cut(s) 497, 605, 682, 901
AsuC2I CCSGG 5 cut(s) 155, 156, 680, 781, 829
AsuHPI GGTGA 6 cut(s) 275, 419, 741, 769, 965, 1005
AvaI CYCGRG 1 cut(s) 154
AvaII GGWCC 4 cut(s) 497, 605, 682, 901
BamHI GGATCC 1 cut(s) 559
BanII GRGCYC 1 cut(s) 884
BbsI GAAGAC 1 cut(s) 816
BbvI GCAGC 7 cut(s) 155, 183, 222, 358, 361, 424, 565
BccI CCATC 4 cut(s) 471, 488, 881, 1024
BceAI ACGGC 2 cut(s) 742, 967
BcgI CGANNNNNNTGC 2 cut(s) 160, 194
BciT130I CCWGG 1 cut(s) 540
BcnI CCSGG 5 cut(s) 155, 156, 680, 781, 829
BfaI CTAG 1 cut(s) 278
BfmI CTRYAG 2 cut(s) 169, 208
BglI GCCNNNNNGGC 1 cut(s) 234
BlpI GCTNAGC 1 cut(s) 112
Bme1390I CCNGG 6 cut(s) 155, 156, 540, 680, 781, 829
Bme18I GGWCC 4 cut(s) 497, 605, 682, 901
BmeT110I CYCGRG 1 cut(s) 154
BmgT120I GGNCC 4 cut(s) 497, 605, 682, 901
BmiI GGNNCC 3 cut(s) 561, 791, 802
BmrFI CCNGG 6 cut(s) 155, 156, 540, 680, 781, 829
BmrI ACTGGG 1 cut(s) 480
BmsI GCATC 1 cut(s) 349
BmuI ACTGGG 1 cut(s) 480
BpiI GAAGAC 1 cut(s) 816
Bpu1102I GCTNAGC 1 cut(s) 112
BpuEI CTTGAG 1 cut(s) 293
BpuMI CCSGG 5 cut(s) 155, 156, 680, 781, 829
BsaJI CCNNGG 4 cut(s) 103, 154, 555, 601
BsaWI WCCGGW 1 cut(s) 797
Bsc4I CCNNNNNNNGG 5 cut(s) 139, 341, 426, 427, 786
Bse118I RCCGGY 1 cut(s) 815
Bse1I ACTGG 2 cut(s) 409, 475
Bse3DI GCAATG 3 cut(s) 128, 170, 366
BseAI TCCGGA 1 cut(s) 797
BseBI CCWGG 1 cut(s) 540
BseDI CCNNGG 4 cut(s) 103, 154, 555, 601
BseGI GGATG 4 cut(s) 482, 741, 892, 1016
BseLI CCNNNNNNNGG 5 cut(s) 139, 341, 426, 427, 786
BseMI GCAATG 3 cut(s) 128, 170, 366
BseMII CTCAG 1 cut(s) 126
BseNI ACTGG 2 cut(s) 409, 475
BseRI GAGGAG 2 cut(s) 120, 267
BseXI GCAGC 7 cut(s) 155, 183, 222, 358, 361, 424, 565
BsgI GTGCAG 1 cut(s) 471
Bsh1236I CGCG 1 cut(s) 603
BshFI GGCC 2 cut(s) 65, 600
BsiHKCI CYCGRG 1 cut(s) 154
BsiSI CCGG 6 cut(s) 155, 680, 780, 798, 816, 828
BslFI GGGAC 1 cut(s) 332
BslI CCNNNNNNNGG 5 cut(s) 139, 341, 426, 427, 786
BsmFI GGGAC 1 cut(s) 332
BsmI GAATGC 1 cut(s) 722
BsnI GGCC 2 cut(s) 65, 600
BsoBI CYCGRG 1 cut(s) 154
Bsp1286I GDGCHC 1 cut(s) 884
Bsp13I TCCGGA 1 cut(s) 797
Bsp143I GATC 5 cut(s) 274, 542, 559, 705, 840
Bsp1720I GCTNAGC 1 cut(s) 112
BspACI CCGC 9 cut(s) 234, 237, 240, 258, 390, 601, 603, 899, 904
BspANI GGCC 2 cut(s) 65, 600
BspCNI CTCAG 1 cut(s) 125
BspEI TCCGGA 1 cut(s) 797
BspFNI CGCG 1 cut(s) 603
BspHI TCATGA 1 cut(s) 460
BspLI GGNNCC 3 cut(s) 561, 791, 802
BspMAI CTGCAG 2 cut(s) 173, 212
BspPI GGATC 4 cut(s) 282, 550, 554, 567
BsrDI GCAATG 3 cut(s) 128, 170, 366
BsrFI RCCGGY 1 cut(s) 815
BsrI ACTGG 2 cut(s) 409, 475
BssAI RCCGGY 1 cut(s) 815
BssECI CCNNGG 4 cut(s) 103, 154, 555, 601
BssMI GATC 5 cut(s) 274, 542, 559, 705, 840
Bst2UI CCWGG 1 cut(s) 540
Bst4CI ACNGT 5 cut(s) 326, 551, 642, 860, 929
BstAPI GCANNNNNTGC 2 cut(s) 143, 219
BstDEI CTNAG 2 cut(s) 112, 445
BstDSI CCRYGG 1 cut(s) 601
BstENI CCTNNNNNAGG 1 cut(s) 339
BstF5I GGATG 4 cut(s) 482, 741, 892, 1016
BstFNI CGCG 1 cut(s) 603
BstKTI GATC 5 cut(s) 277, 545, 562, 708, 843
BstMBI GATC 5 cut(s) 274, 542, 559, 705, 840
BstNI CCWGG 1 cut(s) 540
BstSCI CCNGG 6 cut(s) 153, 154, 538, 678, 779, 827
BstSFI CTRYAG 2 cut(s) 169, 208
BstUI CGCG 1 cut(s) 603
BstV1I GCAGC 7 cut(s) 155, 183, 222, 358, 361, 424, 565
BstV2I GAAGAC 1 cut(s) 816
BstX2I RGATCY 3 cut(s) 274, 542, 559
BstXI CCANNNNNNTGG 1 cut(s) 507
BstYI RGATCY 3 cut(s) 274, 542, 559
BsuRI GGCC 2 cut(s) 65, 600
BtgI CCRYGG 1 cut(s) 601
BtsCI GGATG 4 cut(s) 482, 741, 892, 1016
BtsIMutI CAGTG 1 cut(s) 647
CaiI CAGNNNCTG 1 cut(s) 701
CciI TCATGA 1 cut(s) 460
Cfr10I RCCGGY 1 cut(s) 815
Cfr13I GGNCC 4 cut(s) 497, 605, 682, 901
Cfr42I CCGCGG 1 cut(s) 604
Cfr9I CCCGGG 1 cut(s) 154
CpoI CGGWCCG 1 cut(s) 901
Csp6I GTAC 2 cut(s) 837, 925
CspI CGGWCCG 1 cut(s) 901
CviAII CATG 5 cut(s) 49, 461, 507, 573, 767
CviQI GTAC 2 cut(s) 837, 925
DdeI CTNAG 2 cut(s) 112, 445
DpnI GATC 5 cut(s) 276, 544, 561, 707, 842
DpnII GATC 5 cut(s) 274, 542, 559, 705, 840
EaeI YGGCCR 1 cut(s) 598
EciI GGCGGA 1 cut(s) 914
Eco24I GRGCYC 1 cut(s) 884
Eco47I GGWCC 4 cut(s) 497, 605, 682, 901
Eco88I CYCGRG 1 cut(s) 154
EcoNI CCTNNNNNAGG 1 cut(s) 339
EcoRII CCWGG 1 cut(s) 538
EcoT38I GRGCYC 1 cut(s) 884
FaeI CATG 5 cut(s) 52, 464, 510, 576, 770
FalI AAGNNNNNCTT 2 cut(s) 278, 310
FaqI GGGAC 1 cut(s) 332
FatI CATG 5 cut(s) 48, 460, 506, 572, 766
FauI CCCGC 1 cut(s) 397
FokI GGATG 4 cut(s) 489, 748, 899, 1003
FriOI GRGCYC 1 cut(s) 884
FspBI CTAG 1 cut(s) 278
HaeIII GGCC 2 cut(s) 65, 600
HapII CCGG 6 cut(s) 155, 680, 780, 798, 816, 828
Hin1II CATG 5 cut(s) 52, 464, 510, 576, 770
HinfI GANTC 1 cut(s) 996
HpaII CCGG 6 cut(s) 155, 680, 780, 798, 816, 828
HphI GGTGA 6 cut(s) 275, 419, 741, 769, 965, 1005
Hpy166II GTNNAC 1 cut(s) 863
Hpy188I TCNGA 4 cut(s) 104, 559, 886, 978
Hpy188III TCNNGA 6 cut(s) 461, 715, 733, 798, 919, 993
Hpy8I GTNNAC 1 cut(s) 863
Hpy99I CGWCG 2 cut(s) 326, 763
HpyAV CCTTC 3 cut(s) 394, 721, 804
HpyCH4III ACNGT 5 cut(s) 326, 551, 642, 860, 929
HpyCH4V TGCA 3 cut(s) 171, 210, 488
HpyF3I CTNAG 2 cut(s) 112, 445
Hsp92II CATG 5 cut(s) 52, 464, 510, 576, 770
Kpn2I TCCGGA 1 cut(s) 797
KspI CCGCGG 1 cut(s) 604
Kzo9I GATC 5 cut(s) 274, 542, 559, 705, 840
LmnI GCTCC 2 cut(s) 659, 800
Lsp1109I GCAGC 7 cut(s) 155, 183, 222, 358, 361, 424, 565
LweI GCATC 1 cut(s) 349
MaeI CTAG 1 cut(s) 278
MaeIII GTNAC 2 cut(s) 263, 953
MalI GATC 5 cut(s) 276, 544, 561, 707, 842
MboI GATC 5 cut(s) 274, 542, 559, 705, 840
MboII GAAGA 1 cut(s) 816
MflI RGATCY 3 cut(s) 274, 542, 559
MhlI GDGCHC 1 cut(s) 884
MluCI AATT 2 cut(s) 565, 625
MlyI GAGTC 1 cut(s) 990
MroI TCCGGA 1 cut(s) 797
MseI TTAA 3 cut(s) 96, 270, 522
MslI CAYNNNNRTG 1 cut(s) 505
MspA1I CMGCKG 1 cut(s) 603
MspI CCGG 6 cut(s) 155, 680, 780, 798, 816, 828
MspR9I CCNGG 6 cut(s) 155, 156, 540, 680, 781, 829
Mva1269I GAATGC 1 cut(s) 722
MvaI CCWGG 1 cut(s) 540
MvnI CGCG 1 cut(s) 603
NciI CCSGG 5 cut(s) 155, 156, 680, 781, 829
NdeII GATC 5 cut(s) 274, 542, 559, 705, 840
NlaIII CATG 5 cut(s) 52, 464, 510, 576, 770
NlaIV GGNNCC 3 cut(s) 561, 791, 802
NmuCI GTSAC 2 cut(s) 263, 953
PagI TCATGA 1 cut(s) 460
PctI GAATGC 1 cut(s) 722
PfoI TCCNGGA 1 cut(s) 538
PleI GAGTC 1 cut(s) 990
PpsI GAGTC 1 cut(s) 990
Psp6I CCWGG 1 cut(s) 538
PspGI CCWGG 1 cut(s) 538
PspN4I GGNNCC 3 cut(s) 561, 791, 802
PspPI GGNCC 4 cut(s) 497, 605, 682, 901
PstI CTGCAG 2 cut(s) 173, 212
PstNI CAGNNNCTG 1 cut(s) 701
PsuI RGATCY 3 cut(s) 274, 542, 559
RsaI GTAC 2 cut(s) 838, 926
RsaNI GTAC 2 cut(s) 837, 925
RseI CAYNNNNRTG 1 cut(s) 505
Rsr2I CGGWCCG 1 cut(s) 901
RsrII CGGWCCG 1 cut(s) 901
SacII CCGCGG 1 cut(s) 604
SaqAI TTAA 3 cut(s) 96, 270, 522
Sau3AI GATC 5 cut(s) 274, 542, 559, 705, 840
Sau96I GGNCC 4 cut(s) 497, 605, 682, 901
SchI GAGTC 1 cut(s) 990
ScrFI CCNGG 6 cut(s) 155, 156, 540, 680, 781, 829
SduI GDGCHC 1 cut(s) 884
SetI ASST 6 cut(s) 296, 451, 687, 824, 926, 963
SfaNI GCATC 1 cut(s) 349
SfcI CTRYAG 2 cut(s) 169, 208
Sfr303I CCGCGG 1 cut(s) 604
SgrBI CCGCGG 1 cut(s) 604
SinI GGWCC 4 cut(s) 497, 605, 682, 901
SmaI CCCGGG 1 cut(s) 156
SmiMI CAYNNNNRTG 1 cut(s) 505
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
Sse9I AATT 2 cut(s) 565, 625
SsiI CCGC 9 cut(s) 234, 237, 240, 258, 390, 601, 603, 899, 904
SspMI CTAG 1 cut(s) 278
StyD4I CCNGG 6 cut(s) 153, 154, 538, 678, 779, 827
TaaI ACNGT 5 cut(s) 326, 551, 642, 860, 929
TaqI TCGA 1 cut(s) 1037
TasI AATT 2 cut(s) 565, 625
TauI GCSGC 3 cut(s) 237, 240, 603
Tru1I TTAA 3 cut(s) 96, 270, 522
Tru9I TTAA 3 cut(s) 96, 270, 522
TscAI CASTG 1 cut(s) 647
TseFI GTSAC 2 cut(s) 263, 953
TseI GCWGC 7 cut(s) 168, 171, 210, 371, 374, 437, 578
Tsp45I GTSAC 2 cut(s) 263, 953
TspDTI ATGAA 2 cut(s) 355, 477
TspMI CCCGGG 1 cut(s) 154
TspRI CASTG 1 cut(s) 647
VpaK11BI GGWCC 4 cut(s) 497, 605, 682, 901
XagI CCTNNNNNAGG 1 cut(s) 339
XmaI CCCGGG 1 cut(s) 154
XspI CTAG 1 cut(s) 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.